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https://www.readbyqxmd.com/read/29033290/nonvascularized-cartilage-grafts-versus-vascularized-cartilage-flaps-comparison-of-cartilage-quality-6%C3%A2-months-after-transfer-and-isolated-from-surrounding%C3%A2-bone-ingrowth
#1
James P Higgins, Farzad Borumandi, Heinz K Bürger, Mehmet Emre Benlidayı, Anna Vasilyeva, Leman Sencar, Sait Polat, Alexander J Gaggl
PURPOSE: Subchondral perfusion of osteochondral grafts has been shown to be important in preventing long-term cartilage degeneration. In carpal reconstruction, subchondral perfusion from the graft bed is limited. This study's purpose was to compare the histological characteristics of cartilage in osteochondral grafts supported by synovial imbibition alone to cartilage of vascularized osteochondral flaps that have both synovial and vascular pedicle perfusion. METHODS: Two adjacent osteochondral segments were harvested on the medial femoral trochlea in domestic 6- to 8-month-old pigs...
October 12, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29022110/monitoring-bone-tissue-engineered-bte-constructs-based-on-the-shifting-metabolism-of-differentiating-stem-cells
#2
Aaron D Simmons, Vassilios I Sikavitsas
Ever-increasing demand for bone grafts necessitates the realization of clinical implementation of bone tissue engineered constructs. The predominant hurdle to implementation remains to be securing FDA approval, based on the lack of viable methods for the rigorous monitoring of said constructs. The study presented herein details a method for such monitoring based on the shifting metabolism of mesenchymal stem cells (MSCs) as they differentiate into osteoblasts. To that end, rat MSCs seeded on 85% porous spunbonded poly(L-lactic acid) scaffolds were cultured in flow perfusion bioreactors with baseline or osteoinductive media, and levels of key physio-metabolic markers (oxygen, glucose, osteoprotegerin, and osteocalcin) were monitored throughout culture...
October 11, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28989148/-effect-of-growth-hormone-on-osteopontin-expression-during-orthodontic-tooth-movement-in-rats
#3
Hualong Ju, Xiuying Cai
To investigate the effect of growth hormone on osteopontin expression during orthodontic tooth movement in rats.
 Methods: Forty male Wistar rats (2 weeks, average weight 200 g) were randomly divided into a control group and an experimental group (n=20 per group). The experimental group received subcutaneous injections of growth hormone at a dose of 0.15 U/(kg.d), and the control group received equivalent volumes of saline. A nickel-titanium spring was fixed between the maxillary incisors and the left upper first molar with a force of 0...
September 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28978662/fractalkine-induces-angiogenic-potential-in-cx3cr1-expressing-monocytes
#4
Youngrok Park, Joon Lee, Jae-Yong Kwak, Kyoungmi Noh, Eunjung Yim, Hyun-Kyung Kim, Young June Kim, Hal E Broxmeyer, Jeong-A Kim
We report the unique role of CX3CL1 (or fractalkine) on CD11b(+) myelomonocytic cells expressing CX3CR1, the only known receptor for CX3CL1, in promoting blood perfusion recovery. In a mouse ischemic hind-limb model, CD11b(+)CX3CR1(+) cells migrated to ischemic femoral muscles through CX3CL1-mediated chemotaxis. CD11b(+)CX3CR1(+) macrophages isolated from ischemic tissues [tissue (T)-CD11b(+)CX3CR1(+)] of muscle exert a proangiogenic effect through platelet factor-4 (CXCL4; PF-4) production. PF-4 does not promote angiogenesis by itself but, instead, increases VEGF-mediated angiogenesis...
October 4, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28978523/recombinant-sclerostin-antagonises-effects-of-ex-vivo-mechanical-loading-in-trabecular-bone-and-increases-osteocyte-lacunar-size
#5
Masakazu Kogawa, Kamarul A Khalid, Asiri R Wijenayaka, Renee T Ormsby, Andreas Evdokiou, Paul H Anderson, David M Findlay, Gerald J Atkins
Sclerostin (SCL) has emerged as an important regulator of bone mass. We have shown that SCL can act by targeting late osteoblasts/osteocytes to inhibit bone mineralisation and to upregulate osteocyte expression of catabolic factors, resulting in osteocytic osteolysis. Here we sought to examine the effect of exogenous sclerostin on osteocytes in trabecular bone mechanically loaded ex vivo. Bovine trabecular bone cores, with bone marrow removed, were inserted into individual chambers and subjected to daily episodes of dynamic loading...
October 4, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28976370/-what-is-aging-and-cardiovascular-aging
#6
Z Lale Koldaş
Aging is one of the main risk factors for cardiovascular disease (CVD) and results in a progressive functional decline of organs and the vasculature. According to WHO data today, 8.5 percent of people worldwide (617 million) are aged 65 and over, this percentage is projected to jump to nearly 17 percent of the world's population by 2050 (1.6 billion). Turkey's 65-and- over population rise over the last 5 years about 17,1% and it is estimated that the percentage of this elderly population will rise from 8.5% (2016 data) to 20,8% in the year 2050...
September 2017: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
https://www.readbyqxmd.com/read/28955160/signal-changes-in-standing-magnetic-resonance-imaging-of-osseous-injury-at-the-origin-of-the-suspensory-ligament-in-four-thoroughbred-racehorses-under-tiludronic-acid-treatment
#7
Fumiaki Mizobe, Motoi Nomura, Tomohiro Kato, Yasuo Nambo, Kazutaka Yamada
Problems associated with the proximal metacarpal region, such as an osseous injury associated with tearing of Sharpey's fibers or an avulsion fracture of the origin of the suspensory ligament (OISL), are important causes of lameness in racehorses. In the present study, four Thoroughbred racehorses (age range, 2-4 years) were diagnosed as having forelimb OISL and assessed over time by using standing magnetic resonance imaging (sMRI). At the first sMRI examination, all horses had 3 characteristic findings, including low signal intensity within the trabecular bone of the third metacarpus on T1-weighted images, intermediate-to-high signal intensity surrounded by a hypointense rim on T2*-weighted images, and high signal intensity on fat-suppressed images...
2017: Journal of Equine Science
https://www.readbyqxmd.com/read/28942121/ex-vivo-construction-of-human-primary-3d-networked-osteocytes
#8
Qiaoling Sun, Saba Choudhary, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Y Lee
A human bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via the biomimetic assembly of primary human osteoblastic cells with 20-25μm microbeads and subsequent microfluidic perfusion culture. The biomimetic assembly: (1) enabled 3D-constructed cells to form cellular network via processes with an average cell-to-cell distance of 20-25μm, and (2) inhibited cell proliferation within the interstitial confine between the microbeads while the confined cells produced extracellular matrix (ECM) to form a mechanically integrated structure...
September 21, 2017: Bone
https://www.readbyqxmd.com/read/28917708/type-2-diabetes-mellitus-balb-c-mice-are-more-susceptible-to-granulomatous-amoebic-encephalitis-immunohistochemical-study
#9
Maritza Omaña-Molina, Raquel Sanchez-Rocha, Dolores Hernandez-Martinez, Miriam Romero Grijalva, Citlaltepetl Salinas-Lara, Miriam Rodriguez-Sosa, Imelda Juarez-Avelar, Lizbeth Salazar-Villatoro, Arturo Gonzalez-Robles, Adolfo Rene Mendez-Cruz, Patricia Aley-Medina, Jesus Espinosa-Villanueva, Ismael Castelan-Ramirez, Jacob Lorenzo-Morales
Granulomatous amoebic encephalitis (GAE) is a chronic, difficult to resolve infection caused by amphizoic amoebae of the genus Acanthamoeba, which in most cases occurs in immunosuppressed persons or with chronic diseases such as diabetes. In this study, we describe the early events of A. culbertsoni infection of GAE in diabetic mice model. Diabetes was induced in male BALB/c mice, with a dose of streptozotocin (130 mg/kg). Healthy and diabetic mice were inoculated via intranasal with 1 × 10(6) trophozoites of A...
September 13, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28899758/chronic-global-analysis-of-vascular-permeability-and-cerebral-blood-flow-after-bone-marrow-stromal-cell-treatment-of-traumatic-brain-injury-in-the-rat-a-long-term-mri-study
#10
Lian Li, Michael Chopp, Guangliang Ding, Qingjiang Li, Asim Mahmood, Quan Jiang
Vascular permeability and hemodynamic alteration in response to the transplantation of human bone marrow stromal cells (hMSCs) after traumatic brain injury (TBI) were longitudinally investigated in non directly injured and normal-appearing cerebral tissue using magnetic resonance imaging (MRI). Male Wistar rats (300-350g, n=30) subjected to controlled cortical impact TBI were intravenously injected with 1ml of saline (at 6-h or 1-week post-injury, n=5/group) or with hMSCs in suspension (∼3×10(6) hMSCs, at 6-h or 1-week post-injury, n=10/group)...
November 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28898758/modular-flow-chamber-for-engineering-bone-marrow-architecture-and-function
#11
Christian A Di Buduo, Paolo M Soprano, Lorenzo Tozzi, Stefania Marconi, Ferdinando Auricchio, David L Kaplan, Alessandra Balduini
The bone marrow is a soft, spongy, gelatinous tissue found in the hollow cavities of flat and long bones that support hematopoiesis in order to maintain the physiologic turnover of all blood cells. Silk fibroin, derived from Bombyx mori silkworm cocoons, is a promising biomaterial for bone marrow engineering, because of its tunable architecture and mechanical properties, the capacity of incorporating labile compounds without loss of bioactivity and demonstrated ability to support blood cell formation. In this study, we developed a bone marrow scaffold consisting of a modular flow chamber made of polydimethylsiloxane, holding a silk sponge, prepared with salt leaching methods and functionalized with extracellular matrix components...
November 2017: Biomaterials
https://www.readbyqxmd.com/read/28898756/bioreactor-culture-duration-of-engineered-constructs-influences-bone-formation-by-mesenchymal-stem-cells
#12
Debika Mitra, Jacklyn Whitehead, Osamu W Yasui, J Kent Leach
Perfusion culture of mesenchymal stem cells (MSCs) seeded in biomaterial scaffolds provides nutrients for cell survival, enhances extracellular matrix deposition, and increases osteogenic cell differentiation. However, there is no consensus on the appropriate perfusion duration of cellular constructs in vitro to boost their bone forming capacity in vivo. We investigated this phenomenon by culturing human MSCs in macroporous composite scaffolds in a direct perfusion bioreactor and compared their response to scaffolds in continuous dynamic culture conditions on an XYZ shaker...
November 2017: Biomaterials
https://www.readbyqxmd.com/read/28894668/mucosal-perfusion-preservation-by-a-novel-shapeable-tissue-expander-for-oral-reconstruction
#13
Daria Barwinska, John Garner, Darrell D Davidson, Todd G Cook, George J Eckert, Sunil S Tholpady, Keith L March, Kinam Park, Clark T Barco
BACKGROUND: There are few methods for expanding oral mucosa, and these often cause complications such as tissue necrosis and expander eruption. This study examines mucosal blood perfusion following insertion of a novel shapeable hydrogel tissue expander (HTE). The canine model used subgingival insertion of HTE following tooth extraction and alveolar bone reduction. The primary goal of this study was to gain understanding of epithelial perfusion and reparative responses of gingival mucosa during HTE expansion...
August 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28893630/engineered-axially-vascularized-osteogenic-grafts-from-human-adipose-derived-cells-to-treat-avascular-necrosis-of-bone-in-a-rat-model
#14
Tarek Ismail, Rik Osinga, Atanas Todorov, Alexander Haumer, Laurent A Tchang, Christian Epple, Nima Allafi, Nadia Menzi, René D Largo, Alexandre Kaempfen, Ivan Martin, Dirk J Schaefer, Arnaud Scherberich
BACKGROUND: Avascular necrosis of bone (AVN) leads to sclerosis and collapse of bone and joints. The standard of care, vascularized bone grafts, is limited by donor site morbidity and restricted availability. The aim of this study was to generate and test engineered, axially vascularized SVF cells-based bone substitutes in a rat model of AVN. METHODS: SVF cells were isolated from lipoaspirates and cultured onto porous hydroxyapatite scaffolds within a perfusion-based bioreactor system for 5 days...
September 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28881093/expansion-of-bone-marrow-mesenchymal-stromal-cells-in-perfused-3d-ceramic-scaffolds-enhances-in-vivo-bone-formation
#15
Allison I Hoch, Ralph Duhr, Nunzia Di Maggio, Arne Mehrkens, Marcel Jakob, David Wendt
Bone marrow-derived mesenchymal stromal cells (BMSC), when expanded directly within 3D ceramic scaffolds in perfusion bioreactors, more reproducibly form bone when implanted in vivo as compared to conventional expansion on 2D polystyrene dishes/flasks. Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, we aimed to decouple the effects of specific parameters among these two model systems. We assessed the effects of the: 1) 3D scaffold vs...
September 7, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28872256/deformation-strain-is-the-main-physical-driver-for-skeletal-precursors-to-undergo-osteogenesis-in-earlier-stages-of-osteogenic-cell-maturation
#16
Ram-Kumar Ramani-Mohan, Ivo Schwedhelm, Anna Finne-Wistrand, Melanie Krug, Thomas Schwarz, Franz Jakob, Heike Walles, Jan Hansmann
Mesenchymal stem cells play a major role during bone remodeling and are thus of high interest for tissue engineering and regenerative medicine applications. Mechanical stimuli, i.e. deformation strain and interstitial fluid-flow-induced shear stress, promote osteogenic lineage commitment. However, the predominant physical stimulus that drives early osteogenic cell maturation is not clearly identified. The evaluation of each stimulus is challenging, as deformation and fluid-flow-induced shear stress interdepend...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28869710/influence-of-hydrodynamic-pressure-on-the-proliferation-and-osteogenic-differentiation-of-bone-mesenchymal-stromal-cells-seeded-on-polyurethane-scaffolds
#17
Xiangyu Tang, Songsong Teng, Chaoxu Liu, Michael Jagodzinski
Hydraulic pressure has recently been introduced as an effective stimulation in the field of tissue engineering. In the present study, a polymer scaffold consisting of polyurethane (PU)-based 1, 4-butanediisocyanate (BDI) was fabricated. A self-designed bioreactor was employed to produce perfusion and hydrodynamic pressure stimulations. The viability, proliferation and osteogenic differentiation of the rat bone mesenchymal stromal cell (rBMSC) growing in the polymer scaffold were investigated after hydrodynamic pressure stimulation...
September 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28868021/a-case-of-recurrent-breast-cancer-identified-by-pulmonary-tumor-thrombotic-microangiopathy
#18
Tomomi Abe, Ippei Fukada, Taro Shiga, Hidetomo Morizono, Koichi Ikebata, Tomoko Shibayama, Kokoro Kobayashi, Takuji Iwase, Shinji Ohno, Yoshinori Ito
Pulmonary tumor thrombotic microangiopathy (PTTM) is a rare, cancer-related, pulmonary complication that causes hypoxia and pulmonary hypertension. We report on a 42-year-old woman who was diagnosed with recurrent breast cancer that was detected due to the presence of PTTM. Eleven months after surgery for heterochronous bilateral cancer of the left breast, she developed progressive dyspnea but computerized tomography showed no pulmonary thromboembolism, and a transthoracic echocardiography revealed mild pulmonary hypertension...
May 2017: Case Reports in Oncology
https://www.readbyqxmd.com/read/28830269/adhesion-proliferation-and-osteogenic-differentiation-of-human-mscs-cultured-under-perfusion-with-a-marine-oxygen-carrier-on-an-allogenic-bone-substitute
#19
Fiona Le Pape, Gaëlle Richard, Emmanuelle Porchet, Sophie Sourice, Frédéric Dubrana, Claude Férec, Valérie Polard, Richard Pace, Pierre Weiss, Franck Zal, Pascal Delépine, Elisabeth Leize
Tissue engineering strategies have been developed to optimize osseointegration in dental implant surgery. One of the major problems is the non-homogeneous spatial cell distribution in the scaffold, as well as subsequent matrix production. Insufficient nutrient and oxygen supplies inside the scaffold are factors in this phenomenon. To mediate this gradient formation, we have implemented a perfusion culture method to seed human bone marrow mesenchymal stem cells (MSCs) into three-dimensional (3-D)-allogenic bone scaffolds in combination with a marine haemoglobin, HEMOXCell(®), for oxygen delivery...
August 22, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28816358/trpc3-mediated-ca-2-signals-as-a-promising-strategy-to-boost-therapeutic-angiogenesis-in-failing-hearts-the-role-of-autologous-endothelial-colony-forming-cells
#20
REVIEW
Francesco Moccia, Angela Lucariello, Germano Guerra
Endothelial progenitor cells (EPCs) are a sub-population of bone marrow-derived mononuclear cells that are released in circulation to restore damaged endothelium during its physiological turnover or rescue blood perfusion after an ischemic insult. Additionally, they may be mobilized from perivascular niches located within larger arteries' wall in response to hypoxic conditions. For this reason, EPCs have been regarded as an effective tool to promote revascularization and functional recovery of ischemic hearts, but clinical application failed to exploit the full potential of patients-derived cells...
August 17, 2017: Journal of Cellular Physiology
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