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https://www.readbyqxmd.com/read/29441719/azd1208-a-pan-pim-kinase-inhibitor-inhibits-adipogenesis-and-induces-lipolysis-in-3t3-l1-adipocytes
#1
Yu-Kyoung Park, Brice Wilfried Obiang-Obounou, Kyung-Bok Lee, Jong-Soon Choi, Byeong-Churl Jang
The proviral integration moloney murine leukaemia virus (Pim) kinases, consisting of Pim-1, Pim-2 and Pim-3, are involved in the control of cell growth, metabolism and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. AZD1208 is a pan-Pim kinase inhibitor and is known for its anti-cancer activity. In this study, we investigated the effect of AZD1208 on adipogenesis and lipolysis in 3T3-L1 cells, a murine preadipocyte cell line. AZD1208 markedly suppressed lipid accumulation and reduced triglyceride contents in differentiating 3T3-L1 cells, suggesting the drug's anti-adipogenic effect...
February 14, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29414921/microrna-125a-5p-affects-adipocytes-proliferation-differentiation-and-fatty-acid-composition-of-porcine-intramuscular-fat
#2
Jingjing Du, Yan Xu, Peiwen Zhang, Xue Zhao, Mailin Gan, Qiang Li, Jideng Ma, Guoqing Tang, Yanzhi Jiang, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipogenesis. Previously, microRNA-125a-5p (miR-125a-5p) was identified as an important regulator of adipogenesis. In the present study, we found that the expression of miR-125a-5p is dynamically regulated during porcine intramuscular preadipocytes differentiation and that its expression levels in different porcine muscle tissues were negatively involved with IMF content...
February 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29410257/plasticizers-used-in-food-contact-materials-affect-adipogenesis-in-3t3-l1-cells
#3
Valentina Pomatto, Erika Cottone, Paolo Cocci, Matteo Mozzicafreddo, Gilberto Mosconi, Erik Russel Nelson, Francesco Alessandro Palermo, Patrizia Bovolin
Recent studies suggest that exposure to some plasticizers, such as Bisphenol A (BPA), play a role in endocrine/metabolic dispruption and can affect lipid accumulation in adipocytes. Here, we investigated the adipogenic activity and nuclear receptor interactions of four plasticizers approved for the manufacturing of food-contact materials (FCMs) and currently considered safer alternatives. Differentiating 3T3-L1 mouse preadipocytes were exposed to scalar concentrations (0.01-25 μM) of DiNP (Di-iso-nonyl-phthalate), DiDP (Di-iso-decyl-phthalate), DEGDB (Diethylene glycol dibenzoate), or TMCP (Tri-m-cresyl phosphate)...
February 2, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29396442/nonthermal-plasma-treated-solution-inhibits-adipocyte-differentiation-and-lipogenesis-in-3t3-l1-preadipocytes-via-er-stress-signal-suppression
#4
Sung Un Kang, Haeng Jun Kim, Dae Ho Kim, Chang Hak Han, Yun Sang Lee, Chul-Ho Kim
The accumulation and differentiation of adipocytes contribute to the development of obesity and metabolic diseases. It is well-known that interactions of transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and endoplasmic reticulum (ER) stress are required for adipogenesis. Recently, use of nonthermal atmospheric plasma (NTP) is expanding from the biomedical field into various other fields. In this study, we investigated whether nonthermal plasma-treated solution (NTS) has an inhibitory effect on adipogenesis and elucidated its mechanisms...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29393878/microrna-125a-5p-mediates-3t3-l1-preadipocyte-proliferation-and-differentiation
#5
Yan Xu, Jingjing Du, Peiwen Zhang, Xue Zhao, Qiang Li, Anan Jiang, Dongmei Jiang, Guoqing Tang, Yanzhi Jiang, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Excessive accumulation of adipose tissue is a main cause of obesity or overweight, which is significantly involved in increasing the risk of diseases. Recently, numerous studies have proved that microRNAs (miRNAs) play important roles in adipogenesis by negatively regulating gene expression at posttranscriptional levels. In this study, we showed that miR-125a-5p was expressed at lower levels in the adipose tissues of high-fat diet (HFD)-fed mice than the normal chow (NCW)-fed mice. MiR-125a-5p expression were strongly up-regulated by nearly five-fold, when 3T3-L1 preadipocyte were induced and differentiated into mature adipocytes...
February 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29371997/inhibition-of-adipocyte-differentiation-by-anthocyanins-isolated-from-the-fruit-of-vitis-coignetiae-pulliat-is-associated-with-the-activation-of-ampk-signaling-pathway
#6
Min Ho Han, Hong Jae Kim, Jin-Woo Jeong, Cheol Park, Byung Woo Kim, Yung Hyun Choi
Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production...
January 2018: Toxicological Research
https://www.readbyqxmd.com/read/29358130/ononitol-monohydrate-enhances-prdm16-ucp-1-expression-mitochondrial-biogenesis-and-insulin-sensitivity-via-stat6-and-ltb4r-in-maturing-adipocytes
#7
P Subash-Babu, Ali A Alshatwi
Ononitol monohydrate (OMH), a glycoside was originally isolated from Cassia tora (Linn.). Glycosides regulate lipid metabolism but scientific validation desired. Hence, we aimed to evaluate the effect of OMH on enhancing mitochondrial potential, mitochondrial biogenesis, upregulate the expression of brown adipogenesis specific genes in maturing adipocytes. In addition, we observed the inter-relation between adipocyte and T-lymphocyte; whether, OMH treated adipocyte-condition medium stimulate T-cell chemokine linked with insulin resistance...
January 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29344656/liraglutide-suppresses-proliferation-and-induces-adipogenic-differentiation-of-3t3-l1-cells-via-the-hippo-yap-signaling-pathway
#8
Yongmei Li, Jianying Du, Endong Zhu, Juanjuan Zhang, Jie Han, Wei Zhao, Bei Sun, Derun Tian
Liraglutide, as a glucagon-like peptide‑1 analogue, is used to treat type 2 diabetes mellitus and obesity. Previous findings have demonstrated the effects of liraglutide on adipogenesis; however, the underlying mechanism involved in this process remains to be elucidated. In the present study, to certify the effect of liraglutide on adipogenesis and explore the possible underlying mechanism involved in this process, preadipocyte 3T3‑L1 cells were cultured in adipocyte‑inducing medium and treated with liraglutide...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29339180/leptin-blocks-the-inhibitory-effect-of-vitamin-d-on-adipogenesis-and-cell-proliferation-in-3t3-l1-adipocytes
#9
Jessica Lopes Nobre, Patricia Cristina Lisboa, Janaine Cavalcanti Carvalho, Mariana Renovato Martins, Simone Vargas, Cristina Barja-Fidalgo, Egberto Gaspar de Moura, Elaine de Oliveira
Recently, we demonstrated high serum leptin and 25(OH)D (calcidiol) in obese animals, with high C/EBPβ and PPARγ expression in adipose tissue. Since the role of vitamin D in adipogenesis remains controversial and hyperleptinemia is found in obesity, we asked if leptin could interfere in vitamin D action on adipocytes. Here, we studied the direct effect of these two hormones upon 3T3L1 preadipocytes incubated with or without 1,25(OH)2D (100 nM, 24 h) and with leptin (10-7M, 4 h later). RT-PCR (VDR and Cyp27b1/1α-hydroxylase), western blotting (VDR, Cyp27b1/1α-hydroxylase, ObR-b, C/EBPβ, PPARγ and Bax content), a cell proliferation assay and an Annexin V-FITC binding assay were performed...
January 12, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29334664/methyl-dehydroabietate-counters-high-fat-diet-induced-insulin-resistance-and-hepatic-steatosis-by-modulating-peroxisome-proliferator-activated-receptor-signaling-in-mice
#10
Hiroki Yoshioka, Yuki Mizuno, Tomohiro Yamaguchi, Yoshimi Ichimaru, Koichi Takeya, Yukio Hitotsuyanagi, Tsunemasa Nonogaki, Yutaka Aoyagi
The aim of this study was to investigate the therapeutic effects of methyl dehydroabietate (mDA) on adipocyte differentiation in 3T3-L1 preadipocytes and obesity characteristics induced by high-fat diet (HFD) in mice. Adipocyte differentiation in 3T3-L1 cells was evaluated after 14 days of incubation with mDA. mDA enhanced adipocyte differentiation in 3T3-L1 cells. For the in vivo evaluation, five-week-old male C57BL/6J mice were fed HFD or normal CE-2 diet (control) for eight weeks. During the experimental period, mice were administered mDA (50 mg/kg, p...
January 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29295544/bee-venom-suppresses-the-differentiation-of-preadipocytes-and-high-fat-diet-induced-obesity-by-inhibiting-adipogenesis
#11
Se-Yun Cheon, Kyung-Sook Chung, Seong-Soo Roh, Yun-Yeop Cha, Hyo-Jin An
Bee venom (BV) has been widely used in the treatment of certain immune-related diseases. It has been used for pain relief and in the treatment of chronic inflammatory diseases. Despite its extensive use, there is little documented evidence to demonstrate its medicinal utility against obesity. In this study, we demonstrated the inhibitory effects of BV on adipocyte differentiation in 3T3-L1 cells and on a high fat diet (HFD)-induced obesity mouse model through the inhibition of adipogenesis. BV inhibited lipid accumulation, visualized by Oil Red O staining, without cytotoxicity in the 3T3-L1 cells...
December 24, 2017: Toxins
https://www.readbyqxmd.com/read/29274819/proliferation-metabolic-activity-and-adipogenic-differentiation-of-human-preadipocytes-exposed-to-two-surfactants-in-vitro
#12
Tim Ruhl, Gabriele Storti, Norbert Pallua
Fat grafting is a pivotal technique for tissue repair. Adipose stromal cells, including preadipocytes, play a major role in the regenerative effects attributed to fat grafting. But the benefits are impaired by the low survival of the graft due to mechanical stress during harvesting, hypoxia and nutrient deprivation. Non-ionic surfactant molecules demonstrated their efficacy in preventing and repairing mechanical damage on the cellular membrane, but it is poorly understood if and how they affect cellular viability, proliferation and differentiation...
December 21, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29239820/all-trans-retinoic-acid-induces-mitochondria-mediated-apoptosis-of-human-adipose-derived-stem-cells-and-affects-the-balance-of-the-adipogenic-differentiation
#13
Laynna de Carvalho Schweich, Edwin José Torres de Oliveira, João Renato Pesarini, Larissa Corrêa Hermeto, Melissa Camassola, Nance Beyer Nardi, Themis Maria Milan Brochado, Andréia Conceição Milan Brochado Antoniolli-Silva, Rodrigo Juliano Oliveira
The all-trans-retinoic acid (ATRA) is the most active form of vitamin A that helps to regulate the proliferation, differentiation and apoptosis of several types of cells, mainly the adipocytes, and causes weight loss through the reduction of adipogenesis and lipogenesis. In this present study we demonstrated that ATRA concentrations of 20.75, 50 and 100 μM decreased the cell viability in vitro of human adipose-derived stem cells (ADSCs), and in ADSCs during adipogenic differentiation. The cells cycle assessment showed that ATRA increased the cell frequency in Sub-G1 at 4...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29202114/dihydrosanguinarine-enhances-glucose-uptake-in-mouse-3t3-l1-cells
#14
Yit-Lai Chow, Yuko Iwata, Fumihiko Sato
Recently, more studies have aimed at identifying selective peroxisome proliferator-activated receptor gamma (PPARγ) modulators that transactivate the expression of PPARγ-dependent genes as partial agonists to improve diabetic symptoms with fewer side effects compared to classic PPARγ agonists such as thiazolidinediones. We found that dihydrosanguinarine (DHS) treatment induced preadipocyte differentiation and lipid droplet accumulation in 3T3-L1 cells, but this effect is weaker than that elicited by the full PPARγ agonist troglitazone...
October 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/29181403/gosb-inhibits-triacylglycerol-synthesis-and-promotes-cell-survival-in-mouse-mammary-epithelial-cells
#15
Gaoxiao Xu, Saixing Duan, Jianye Hou, Zhongxin Wei, Guangwei Zhao
It has been demonstrated that the activator protein related transcription factor Finkel-Biskis-Jinkins murine osteosarcoma B (GosB) is involved in preadipocyte differentiation and triacylglycerol synthesis. However, the role of GosB in regulating the synthesis of milk fatty acid in mouse mammary glands remains unclear. This research uncovered potentially new roles of GosB in suppressing milk fatty acid synthesis. Results revealed that GosB had the highest expression in lung tissue and showed a higher expression level during nonlactation than during lactation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29171111/knockdown-of-ubiquitin-d-inhibits-adipogenesis-during-the-differentiation-of-porcine-intramuscular-and-subcutaneous-preadipocytes
#16
Chen Zhao, Xiangping Yao, Xiaochang Chen, Wenjing Wu, Fengxue Xi, Gongshe Yang, Taiyong Yu
OBJECTIVES: Intramuscular fat (IMF) has a significant influence on porcine meat quality. Ubiquitin D (UBD) is involved in the management of diverse intracellular processes. However, its physiological functions in adipose cell differentiation and proliferation are still poorly defined. MATERIALS AND METHODS: Intramuscular and subcutaneous preadipocytes were isolated from the longissimus dorsi and neck subcutaneous deposits of Chinese native Guanzhong Black piglets (3-5 days old), respectively...
November 23, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29168191/genetic-variants-in-il15-promoter-affect-transcription-activity-and-intramuscular-fat-deposition-in-longissimus-dorsi-muscle-of-pigs
#17
D He, Z Jiang, Y Tian, H Han, M Xia, W Wei, L Zhang, J Chen
Intramuscular fat (IMF) content is a key aspect of pork quality. Elucidation of intramuscular adipocyte regulation mechanisms is important for improving IMF content. Intramuscular adipocytes are dispersed among muscle fibers, so they are inclined to be affected by muscle-derived factors. Interleukin-15 is a major muscle-secreted factor. In this study, the genetic and physiological impacts of IL15 on adipogenesis is investigated. The promoter region of IL15 was scanned by comparative sequencing using two DNA pools of high- and low-IMF individuals...
November 22, 2017: Animal Genetics
https://www.readbyqxmd.com/read/29165364/the-rosiglitazone-like-effects-of-vitexilactone-a-constituent-from-vitex-trifolia-l-in-3t3-l1-preadipocytes
#18
Atsuyoshi Nishina, Masaya Itagaki, Daisuke Sato, Hirokazu Kimura, Yasuaki Hirai, Nyunt Phay, Makoto Makishima
The increased number of patients with type 2 diabetes (T2D) has become a worldwide problem, and insulin sensitizers such as thiazolidinediones (TZDs) are used as therapeutic agents. We found that extracts of Vitex trifolia L. (V. trifolia), a medicinal plant from Myanmar, induced adipogenesis similar to rosiglitazone (ROS), which is a TZD, in 3T3-L1 preadipocytes. In the present study, we attempted to isolate from V. trifolia those compounds that showed ROS-like effects. Among the extracts of hexane, ethyl acetate, and methanol obtained from V...
November 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29158522/mir-17-5p-and-mir-20a-promote-chicken-cell-proliferation-at-least-in-part-by-upregulation-of-c-myc-via-map3k2-targeting
#19
Xiaofei Zhang, He Song, Shupei Qiao, Jing Liu, Tianyu Xing, Xiaohong Yan, Hui Li, Ning Wang
The miR-17-92 cluster has been well studied in mammals but less extensively studied in birds. Here, we demonstrated that miR-17-92 cluster overexpression promoted the proliferation of DF1 cells and immortalized chicken preadipocytes (ICPA-1), and miR-17-5p and miR-20a, members of the miR-17-92 cluster, targeted MAP3K2. Further analysis showed that MAP3K2 overexpression reduced the proliferation of DF1 and ICPA-1 cells and attenuated the promotive effect of the miR-17-92 cluster on cell proliferation. Downstream gene expression analysis of the MAPK signalling pathway showed that MAP3K2 overexpression decreased c-Myc expression; in contrast, MAP3K2 knockdown using RNA interference and miR-17-92 cluster overexpression increased c-Myc expression...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29152131/microrna-130b-attenuates-dexamethasone-induced-increase-of-lipid-accumulation-in-porcine-preadipocytes-by-suppressing-ppar-%C3%AE-expression
#20
Shifeng Pan, Yixin Cui, Xuan Dong, Tangjie Zhang, Hua Xing
In this study, two experiments were conducted to determine the role of miR-130b in dexamethasone (DEX)-induced lipid accumulation. Porcine preadipocytes were treated with 10(-6) M DEX for 48 h to investigate effects of DEX in lipid accumulation. Next, in order to illustrate the regulatory role of miR-130b on lipid accumulation induced by DEX, miRNA scrambled control (miR-SC), miR-130b overexpression plasmid and miR-130b inhibitor were respectively transfected into porcine preadipocytes at 24 h before DEX treatment for 48 h (miR-SC-DEX, miR-130b-DEX and miR-130b-inhibitor-DEX)...
October 20, 2017: Oncotarget
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