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preadipocyte AND proliferation

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https://www.readbyqxmd.com/read/29239820/all-trans-retinoic-acid-induces-mitochondria-mediated-apoptosis-of-human-adipose-derived-stem-cells-and-affects-the-balance-of-the-adipogenic-differentiation
#1
Laynna de Carvalho Schweich, Edwin José Torres de Oliveira, João Renato Pesarini, Larissa Corrêa Hermeto, Melissa Camassola, Nance Beyer Nardi, Themis Maria Milan Brochado, Andréia Conceição Milan Brochado Antoniolli-Silva, Rodrigo Juliano Oliveira
The all-trans-retinoic acid (ATRA) is the most active form of vitamin A that helps to regulate the proliferation, differentiation and apoptosis of several types of cells, mainly the adipocytes, and causes weight loss through the reduction of adipogenesis and lipogenesis. In this present study we demonstrated that ATRA concentrations of 20.75, 50 and 100 μM decreased the cell viability in vitro of human adipose-derived stem cells (ADSCs), and in ADSCs during adipogenic differentiation. The cells cycle assessment showed that ATRA increased the cell frequency in Sub-G1 at 4...
November 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29202114/dihydrosanguinarine-enhances-glucose-uptake-in-mouse-3t3-l1-cells
#2
Yit-Lai Chow, Yuko Iwata, Fumihiko Sato
Recently, more studies have aimed at identifying selective peroxisome proliferator-activated receptor gamma (PPARγ) modulators that transactivate the expression of PPARγ-dependent genes as partial agonists to improve diabetic symptoms with fewer side effects compared to classic PPARγ agonists such as thiazolidinediones. We found that dihydrosanguinarine (DHS) treatment induced preadipocyte differentiation and lipid droplet accumulation in 3T3-L1 cells, but this effect is weaker than that elicited by the full PPARγ agonist troglitazone...
October 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/29181403/gosb-inhibits-triacylglycerol-synthesis-and-promotes-cell-survival-in-mouse-mammary-epithelial-cells
#3
Gaoxiao Xu, Saixing Duan, Jianye Hou, Zhongxin Wei, Guangwei Zhao
It has been demonstrated that the activator protein related transcription factor Finkel-Biskis-Jinkins murine osteosarcoma B (GosB) is involved in preadipocyte differentiation and triacylglycerol synthesis. However, the role of GosB in regulating the synthesis of milk fatty acid in mouse mammary glands remains unclear. This research uncovered potentially new roles of GosB in suppressing milk fatty acid synthesis. Results revealed that GosB had the highest expression in lung tissue and showed a higher expression level during nonlactation than during lactation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29171111/knockdown-of-ubiquitin-d-inhibits-adipogenesis-during-the-differentiation-of-porcine-intramuscular-and-subcutaneous-preadipocytes
#4
Chen Zhao, Xiangping Yao, Xiaochang Chen, Wenjing Wu, Fengxue Xi, Gongshe Yang, Taiyong Yu
OBJECTIVES: Intramuscular fat (IMF) has a significant influence on porcine meat quality. Ubiquitin D (UBD) is involved in the management of diverse intracellular processes. However, its physiological functions in adipose cell differentiation and proliferation are still poorly defined. MATERIALS AND METHODS: Intramuscular and subcutaneous preadipocytes were isolated from the longissimus dorsi and neck subcutaneous deposits of Chinese native Guanzhong Black piglets (3-5 days old), respectively...
November 23, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29168191/genetic-variants-in-il15-promoter-affect-transcription-activity-and-intramuscular-fat-deposition-in-longissimus-dorsi-muscle-of-pigs
#5
D He, Z Jiang, Y Tian, H Han, M Xia, W Wei, L Zhang, J Chen
Intramuscular fat (IMF) content is a key aspect of pork quality. Elucidation of intramuscular adipocyte regulation mechanisms is important for improving IMF content. Intramuscular adipocytes are dispersed among muscle fibers, so they are inclined to be affected by muscle-derived factors. Interleukin-15 is a major muscle-secreted factor. In this study, the genetic and physiological impacts of IL15 on adipogenesis is investigated. The promoter region of IL15 was scanned by comparative sequencing using two DNA pools of high- and low-IMF individuals...
November 22, 2017: Animal Genetics
https://www.readbyqxmd.com/read/29165364/the-rosiglitazone-like-effects-of-vitexilactone-a-constituent-from-vitex-trifolia-l-in-3t3-l1-preadipocytes
#6
Atsuyoshi Nishina, Masaya Itagaki, Daisuke Sato, Hirokazu Kimura, Yasuaki Hirai, Nyunt Phay, Makoto Makishima
The increased number of patients with type 2 diabetes (T2D) has become a worldwide problem, and insulin sensitizers such as thiazolidinediones (TZDs) are used as therapeutic agents. We found that extracts of Vitex trifolia L. (V. trifolia), a medicinal plant from Myanmar, induced adipogenesis similar to rosiglitazone (ROS), which is a TZD, in 3T3-L1 preadipocytes. In the present study, we attempted to isolate from V. trifolia those compounds that showed ROS-like effects. Among the extracts of hexane, ethyl acetate, and methanol obtained from V...
November 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29158522/mir-17-5p-and-mir-20a-promote-chicken-cell-proliferation-at-least-in-part-by-upregulation-of-c-myc-via-map3k2-targeting
#7
Xiaofei Zhang, He Song, Shupei Qiao, Jing Liu, Tianyu Xing, Xiaohong Yan, Hui Li, Ning Wang
The miR-17-92 cluster has been well studied in mammals but less extensively studied in birds. Here, we demonstrated that miR-17-92 cluster overexpression promoted the proliferation of DF1 cells and immortalized chicken preadipocytes (ICPA-1), and miR-17-5p and miR-20a, members of the miR-17-92 cluster, targeted MAP3K2. Further analysis showed that MAP3K2 overexpression reduced the proliferation of DF1 and ICPA-1 cells and attenuated the promotive effect of the miR-17-92 cluster on cell proliferation. Downstream gene expression analysis of the MAPK signalling pathway showed that MAP3K2 overexpression decreased c-Myc expression; in contrast, MAP3K2 knockdown using RNA interference and miR-17-92 cluster overexpression increased c-Myc expression...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29152131/microrna-130b-attenuates-dexamethasone-induced-increase-of-lipid-accumulation-in-porcine-preadipocytes-by-suppressing-ppar-%C3%AE-expression
#8
Shifeng Pan, Yixin Cui, Xuan Dong, Tangjie Zhang, Hua Xing
In this study, two experiments were conducted to determine the role of miR-130b in dexamethasone (DEX)-induced lipid accumulation. Porcine preadipocytes were treated with 10(-6) M DEX for 48 h to investigate effects of DEX in lipid accumulation. Next, in order to illustrate the regulatory role of miR-130b on lipid accumulation induced by DEX, miRNA scrambled control (miR-SC), miR-130b overexpression plasmid and miR-130b inhibitor were respectively transfected into porcine preadipocytes at 24 h before DEX treatment for 48 h (miR-SC-DEX, miR-130b-DEX and miR-130b-inhibitor-DEX)...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137982/adipose-tissue-complement-factor-b-promotes-adipocyte-maturation
#9
Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Akiko Yamashita, Mitsudai Tsuruta, Shoichiro Nagasaka, Ataru Taniguchi, Mitsuo Fukushima, Naoya Watanabe, Fusanori Nishimura
OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29118419/melatonin-promotes-triacylglycerol-accumulation-via-mt2-receptor-during-differentiation-in-bovine-intramuscular-preadipocytes
#10
Wucai Yang, Keqiong Tang, Yaning Wang, Yingying Zhang, Linsen Zan
Melatonin (N-acetyl-5-methoxytryptamine) is a derivative of tryptophan which is produced and secreted mainly by the pineal gland and regulates a variety of important central and peripheral actions. To examine the potential effects of melatonin on the proliferation and differentiation of bovine intramuscular preadipocytes (BIPs), BIPs were incubated with different concentrations of melatonin. Melatonin supplementation at 1 mM significantly increased peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein (C/EBP) β, and C/EBPα expression and promoted the differentiation of BIPs into adipocytes with large lipid droplets and high cellular triacylglycerol (TAG) levels...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115635/a-potential-role-for-hepcidin-in-obesity-driven-colorectal-tumourigenesis
#11
Elisabeth Phillips, Richard D Horniblow, Vikki Poole, Matthew Bedford, Douglas G Ward, Amanda J Kirkham, Jeremy Tomlinson, Tariq H Iqbal, Chris Tselepis
The obesity epidemic is associated with increases in the incidence of several types of cancer, including colorectal cancer, and is associated with poor outcomes for patients. Adipose tissue is considered biologically active and represents a plausible link between cancer and obesity due to the many factors that it secretes. In the present study, human adipose tissue was cultured in vitro and predifferentiated adipocyte secretome [preadipocyte (PAS)] and differentiated adipocyte secretome (DAS) were collected...
October 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29115433/adiponectin-promotes-preadipocyte-differentiation-via-the-ppar%C3%AE-pathway
#12
Wenkai Yang, Chunli Yang, Jun Luo, Yutao Wei, Wenying Wang, Yingmei Zhong
According to the results of a preliminary study, it was hypothesized that the effects of adiponectin (APN) on the improvement of atherosclerosis may be associated with adipocyte differentiation and peroxisome proliferator‑activated receptor γ (PPARγ). The present study simulated the inflammatory environment of epicardial adipose tissue by stimulating mature adipocytes with lipopolysaccharide (LPS); subsequently, the differentiation of 3T3‑L1 preadipocytes was observed. 3T3‑L1 preadipocytes were infected with an adenovirus containing the human adiponectin gene apM1 (Ad‑apM1) and were co‑cultured with mature adipocytes stimulated with LPS...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29094436/low-level-perfluorooctanoic-acid-enhances-3%C3%A2-t3-l1-preadipocyte-differentiation-via-altering-peroxisome-proliferator-activated-receptor-gamma-expression-and-its-promoter-dna-methylation
#13
Yue Ma, Jie Yang, Yanjian Wan, Yang Peng, Shuai Ding, Yuanyuan Li, Bing Xu, Xi Chen, Wei Xia, Yuebin Ke, Shunqing Xu
Recent studies suggest that perfluorooctanoic acid (PFOA) can play a role in the development of obesity; however, the associated mechanisms are poorly understood. We investigated how PFOA exposure affected the differentiation of 3 T3-L1 preadipocytes and the associated transcriptional and epigenetic mechanisms. Cells treated with different doses of PFOA (ranging from 0.01 to 100 μg ml(-1) ) were assessed for proliferation, differentiation and triglyceride accumulation. The gene expression levels of peroxisome proliferator activated receptor gamma (PPARγ) and its target genes were measured...
November 2, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29082702/-effects-of-mori-folium-extract-on-diet-induced-obesity-mechanism-in-rats
#14
Wen Wu, Kai-Lun Liang, Bo Chen, Jie Su, Su-Hong Chen, Gui-Yuan Lyu
To study the anti-obesity effect of Mori Folium extract on diet-induced obesity(DIO) and to explore the preliminary mechanism in rats. DIO rat models were established by high glucose and high fat diet for 8 weeks. Then high(10 mg•kg⁻¹) and low(5 mg•kg⁻¹) does Mori Folium extracts were given by intragastric administration for 13 weeks. After the last administration, their body weight, 24 h food intake, water intake, Lee's index, liver/body mass index, and fat/body mass index were determined. The levels of lipoprotein lipase(LPL), CCAAT/enhancer-binding protein alpha(C/EBPα) and peroxisome proliferator-activated receptor gamma(PPARγ) were measured by enzyme-linked immunosorbent assay(ELISA)...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29027120/elucidating-the-preadipocyte-and-its-role-in-adipocyte-formation-a-comprehensive-review
#15
REVIEW
Christos N Sarantopoulos, Derek A Banyard, Mary E Ziegler, Beatrice Sun, Ashkaun Shaterian, Alan D Widgerow
Adipogenesis is a complex process whereby the multipotent adipose-derived stem cell is converted to a preadipocyte before terminal differentiation into the mature adipocyte. Preadipocytes are present throughout adult life, exhibit adipose fat depot specificity, and differentiate and proliferate from distinct progenitor cells. The mechanisms that promote preadipocyte commitment and maturation involve numerous protein factor regulators, epigenetic factors, and miRNAs. Detailed characterization of this process is currently an area of intense research and understanding the roles of preadipocytes in tissue plasticity may provide insight into novel approaches for tissue engineering, regenerative medicine and treating a host of obesity-related conditions...
October 13, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29021504/ethanol-extracts-of-aster-yomena-kitam-honda-inhibit-adipogenesis-through-the-activation-of-the-ampk-signaling-pathway-in-3t3-l1-preadipocytes
#16
Min Ho Han, Ji-Suk Jeong, Jin-Woo Jeong, Sung Hyun Choi, Sung Ok Kim, Su Hyun Hong, Cheol Park, Byung Woo Kim, Yung Hyun Choi
The leaves of Aster yomena (Kitam.) Honda have long been used as a traditional herb for treating disorders including coughs, asthma, and insect bites. According to recent studies, A. yomena leaf extracts have several pharmacological properties, including anti-inflammatory, antioxidant, and anti-asthmatic activities. However, little information is available regarding their anti-obesity effect. In this study, we investigated the inhibitory effect of the ethanol extracts of A. yomena leaves (EEAY) on adipocyte differentiation and adipogenesis using 3T3-L1 preadipocytes...
October 11, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28984821/mir-425-5p-inhibits-differentiation-and-proliferation-in-porcine-intramuscular-preadipocytes
#17
Fen-Fen Chen, Yan Xiong, Ying Peng, Yun Gao, Jin Qin, Gui-Yan Chu, Wei-Jun Pang, Gong-She Yang
Intramuscular fat (IMF) content affects the tenderness, juiciness, and flavor of pork. An increasing number of studies are focusing on the functions of microRNAs (miRs) during porcine intramuscular preadipocyte development. Previous studies have proved that miR-425-5p was enriched in porcine skeletal muscles and played important roles in multiple physiological processes; however, its functions during intramuscular adipogenesis remain unclear. To explore the role of miR-425-5p in porcine intramuscular adipogenesis, miR-425-5p agomir and inhibitor were used to perform miR-425-5p overexpression and knockdown in intramuscular preadipocytes, respectively...
October 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28966078/change-in-circulating-microrna-profile-of-obese-children-indicates-future-risk-of-adult-diabetes
#18
Xianwei Cui, Lianghui You, Lijun Zhu, Xing Wang, Yahui Zhou, Yun Li, Juan Wen, Yankai Xia, Xinru Wang, Chenbo Ji, Xirong Guo
PURPOSE: Childhood obesity increases susceptibility to type 2 diabetes (T2D) in adults. Circulating microRNAs (miRNAs) in serum have been proposed as potential diagnostic biomarkers, and they may contribute to the progression toward T2D. Here, we investigated the possibility of predicting the future risk of adult T2D in obese children by using circulating miRNAs. BASIC PROCEDURES: We performed miRNA high-throughput sequencing to screen relevant circulating miRNAs in obese children...
September 28, 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28953247/anti-adipogenic-effects-on-3t3-l1-cells-and-zebrafish-by-tanshinone-iia
#19
Yu-Kyoung Park, Brice Wilfried Obiang-Obounou, Jinho Lee, Tae-Yun Lee, Myung-Ae Bae, Kyu-Seok Hwang, Kyung-Bok Lee, Jong-Soon Choi, Byeong-Churl Jang
Tanshinone IIA is a diterpene quinone isolated from the roots of Salviamiltiorrhiza bunge that has traditionally been used in China for the treatment of cardiovascular and cerebrovascular disorders. Although there is recent evidence showing that tanshinone IIA has an anti-obesity effect, its underlying mechanism of anti-obesity effect is poorly understood. Here, we investigated the effect of tanshinone IIA on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Notably, tanshinone IIA at 10 μM concentration greatly reduced lipid accumulation and triglyceride (TG) contents during 3T3-L1 preadipocyte differentiation, suggesting its anti-adipogenic effect...
September 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28951635/implication-of-ceramide-kinase-in-adipogenesis
#20
Marta Ordoñez, Natalia Presa, Miguel Trueba, Antonio Gomez-Muñoz
Ceramide kinase (CerK) plays a critical role in the regulation of cell growth and survival and has been implicated in proinflammatory responses. In this work, we demonstrate that CerK regulates adipocyte differentiation, a process associated with obesity, which causes chronic low-grade inflammation. CerK was upregulated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Noteworthy, knockdown of CerK using specific siRNA to silence the gene encoding this kinase resulted in substantial decrease of lipid droplet formation and potent depletion in the content of triacylglycerols in the adipocytes...
2017: Mediators of Inflammation
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