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preadipocyte AND proliferation

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https://www.readbyqxmd.com/read/28543688/glycated-human-albumin-alters-mitochondrial-respiration-in-preadipocyte-3t3-l1-cells
#1
Pascal Baret, Fanny Le Sage, Cynthia Planesse, Olivier Meilhac, Anne Devin, Emmanuel Bourdon, Philippe Rondeau
Diabetes and obesity are strongly associated with increased levels of circulating advanced glycation end products (AGEs) and reactive oxygen species (ROS). These two molecular phenomena affect the physiology of adipose tissue, a biological driver of the metabolic syndrome, leading to an inflammatory profile and insulin resistance, which could contribute to obesity/diabetes-associated complications, such as cardiovascular diseases. Herein, we investigated the impact of AGEs on mitochondrial bioenergetics in murine preadipocyte cells (3T3-L1) and cellular redox homeostasis...
May 22, 2017: BioFactors
https://www.readbyqxmd.com/read/28536637/ppar%C3%AE-agonist-through-the-terminal-differentiation-phase-is-essential-for-adipogenic-differentiation-of-fetal-ovine-preadipocytes
#2
Yong Pu, Almudena Veiga-Lopez
BACKGROUND: Although the 3T3-L1 preadipocyte cell line represents an informative model for in vitro adipogenesis research, primary cultured cells are often needed to understand particular human or animal metabolic phenotypes. As demonstrated by in vitro cultured preadipocytes from large mammalian species, primary cultured cells require specific adipogenic differentiation conditions different to that of the 3T3-L1 cell line. These conditions are also species-specific and require optimization steps...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28526415/lnk-deficiency-aggregates-palmitate-induced-preadipocyte-apoptosis
#3
Jie-Yi Du, Chen-Chen Jin, Guo-Hao Wang, Xiong-Qing Huang, Jian-Ding Cheng, Xue-Jun Wen, Xiao-Miao Zhao, Guan-Lei Wang
LNK (SH2B3) is an intracellular adaptor protein that negatively regulates cellular proliferation or self-renewal of hematopoietic stem cells and some other progenitor cells. LNK is also recognized as a key regulator of insulin resistance and inflammatory responses in several tissues and organs. It is unknown the function of LNK in adipose tissue. We previously demonstrated that type 2 diabetes mellitus (T2DM) mouse model had elevated serum free fatty acids (FFAs) levels and increased preadipocyte apoptosis in visceral fat tissue, showing the occurrence of lipotoxicity...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28526050/dbcamp-regulates-adipogenesis-in-sheep-inguinal-preadipocytes
#4
Delin Kong, Jingjing Cui, Juncai Fu
BACKGROUND: The cyclic adenosine-monophosphate (cAMP) pathway is generally recognized as one of the essential pathways for the adipose conversion of rodent preadipocytes in vitro. However, divergent effects of cAMP on adipocyte differentiation have also been reported. Since there is very little data on non-rodent preadipose cells, the aim of the present work was to analyze the effects of one classic activator (dbcAMP) of the cAMP pathway on the proliferation and differentiation of sheep preadipocytes grown...
May 19, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28515350/fam3a-enhances-adipogenesis-of-3t3-l1-preadipocytes-via-activation-of-atp-p2-receptor-akt-signaling-pathway
#5
Yujing Chi, Jing Li, Na Li, Zhenzhen Chen, Liping Ma, Weikang Peng, Xiuying Pan, Mei Li, Weidong Yu, Xiangjun He, Bin Geng, Qinghua Cui, Yulan Liu, Jichun Yang
FAM3A plays important roles in regulating hepatic glucose/lipid metabolism and the proliferation of VSMCs. This study determined the role and mechanism of FAM3A in the adipogenesis of 3T3-L1 preadipocytes. During the adipogenesis of 3T3-L1 preadipocytes, FAM3A expression was significantly increased. FAM3A overexpression enhanced 3T3-L1 preadipocyte adipogenesis with increased phosphorylated Akt (pAkt) level, whereas FAM3A silencing inhibited 3T3-L1 preadipocyte adipogenesis with reduced pAkt level. Moreover, FAM3A silencing reduced the expression and secretion of adipokines in 3T3-L1 cells...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28496206/wisp1-ccn4-inhibits-adipocyte-differentiation-through-repression-of-ppar%C3%AE-activity
#6
Nathalie Ferrand, Véronique Béreziat, Marthe Moldes, Maurice Zaoui, Annette K Larsen, Michèle Sabbah
WISP1 (Wnt1-inducible signaling pathway protein-1, also known as CCN4) is a member of the CCN family able to mediate cell growth, transformation and survival in a tissue-specific manner. Here, we report that WISP1 expression was highly increased in preadipocytes and decreased during adipocyte differentiation. Moreover, we observed an increase in WISP1 gene expression in adipose tissue from both diet-induced and leptin-deficient ob/ob obese mice, suggesting that WISP1 could be involved in the pathophysiological onset of obesity...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28494459/influence-of-bovine-serum-lipids-and-fetal-bovine-serum-on-the-expression-of-cell-surface-markers-in-cultured-bovine-preadipocytes
#7
Mansur A Sandhu, Sandra Jurek, Susanne Trappe, Martin Kolisek, Gerhard Sponder, Jörg R Aschenbach
To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4)...
May 12, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28484500/pingmu-decoction-induces-orbital-preadipocytes-apoptosis-in-vitro
#8
Yali Zhang, Hong Li, Long Gao, Xia Zhang, RuiFang Xie
Pingmu Decoction is the Traditional Chinese Medicine which has treated Graves' Ophthalmopathy (GO) in the inactive stage for more than ten years. This study was to explore the mechanism of Pingmu Decoction of inhibiting preadipocytes in GO patients from differentiating into mature adipocytes. Human orbital preadipocytes were isolated and cultured through tissue explant method. Orbital preadipocytes were induced into mature adipocytes. The medicinal serum was prepared from rats. The cells were treated with medicinal serum which were divided into three groups, low dose group (5%), medium dose group (10%), and high dose group (20%)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28475114/melatonin-and-vitamin-d-interfere-with-the-adipogenic-fate-of-adipose-derived-stem-cells
#9
Valentina Basoli, Sara Santaniello, Sara Cruciani, Giorgio Carlo Ginesu, Maria Laura Cossu, Alessandro Palmerio Delitala, Pier Andrea Serra, Carlo Ventura, Margherita Maioli
Adipose-derived stem cells (ADSCs) represent one of the cellular populations resident in adipose tissue. They can be recruited under certain stimuli and committed to become preadipocytes, and then mature adipocytes. Controlling stem cell differentiation towards the adipogenic phenotype could have a great impact on future drug development aimed at counteracting fat depots. Stem cell commitment can be influenced by different molecules, such as melatonin, which we have previously shown to be an osteogenic inducer...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28472935/gene-expression-profile-during-proliferation-and-differentiation-of-rainbow-trout-adipocyte-precursor-cells
#10
Marta Bou, Jerôme Montfort, Aurélie Le Cam, Cécile Rallière, Véronique Lebret, Jean-Charles Gabillard, Claudine Weil, Joaquim Gutiérrez, Pierre-Yves Rescan, Encarnación Capilla, Isabel Navarro
BACKGROUND: Excessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray. RESULTS: Our analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation...
May 4, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28452592/a-cellular-model-for-the-investigation-of-depot-specific-human-adipocyte-biology
#11
Marijana Todorčević, Catriona Hilton, Catriona McNeil, Constantinos Christodoulides, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick
Upper-body adiposity is associated with increased metabolic disease risk, while lower-body adiposity is paradoxically protective. Efforts to understand the underlying mechanisms require appropriate and reproducible in vitro culture models. We have therefore generated immortalised (im) human preadipocyte (PAD) cell lines derived from paired subcutaneous abdominal and gluteal adipose tissue. These cell lines, denoted imAPAD and imGPAD display enhanced proliferation and robust adipogenic capacities. Differentiated imAPAD and imGPAD adipocytes synthesize triglycerides de novo and respond lipolytically to catecholamine-stimulation...
January 2, 2017: Adipocyte
https://www.readbyqxmd.com/read/28447033/prokineticin-is-a-new-linker-between-obesity-and-cardiovascular-diseases
#12
REVIEW
Canan G Nebigil
Obesity is a fast growing epidemic event worldwide. Fatness is associated with a number of comorbidities, including cardiovascular diseases (CVDs). Although obesity can be heredity in 30-70% cases, the environmental contributions also play an important role in the increasing prevalence of obesity. The relationship between development of obesity and CVD is poorly characterized. Obesity and CVD can also be resulted from a common mechanism such as metabolic, inflammatory, and neurohormonal changes. Prokineticins are defined as cytokines (immunoregulatory proteins), adipokines (adipocyte-secreted hormone), angiogenic (increasing vessel formation), or aneroxic (lowering food intake) hormones...
2017: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28441735/effects-of-flavonoids-and-triterpene-analogues-from-leaves-of-eleutherococcus-sieboldianus-makino-koidz-himeukogi-in-3t3-l1-preadipocytes
#13
Atsuyoshi Nishina, Masaya Itagaki, Yuusuke Suzuki, Mamoru Koketsu, Masayuki Ninomiya, Daisuke Sato, Takashi Suzuki, Satoshi Hayakawa, Makoto Kuroda, Hirokazu Kimura
Eleutherococcus sieboldianus (Makino) Koidz. is a local product from the area in and around Yonezawa City in Yamagata Prefecture, Japan. It has been used as a medicinal plant for a long time. We isolated and identified four types of flavonoid glycosides [astragalin (1), isoquercetin (2), rhamnocitrin 3-O-glucoside (3), and nicotiflorin (4)], a triterpene [methyl hederagenin (5)], and three types of triterpene glycosides [δ-hederin (6), echinocystic acid 3-O-arabinoside (7), and cauloside B (8)] from the methanol extract of E...
April 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28437481/firemaster%C3%A2-550-and-its-components-isopropylated-triphenyl-phosphate-and-triphenyl-phosphate-enhance-adipogenesis-and-transcriptional-activity-of-peroxisome-proliferator-activated-receptor-ppar%C3%AE-on-the-adipocyte-protein-2-ap2-promoter
#14
Emily W Y Tung, Shaimaa Ahmed, Vian Peshdary, Ella Atlas
Firemaster® 550 (FM550) is a chemical mixture currently used as an additive flame retardant in commercial products, and is comprised of 2-ethylhexyl-2,3,4,5-tertrabromobenzoate (TBB), bis(2-ethylhexyl) tetrabromophthalate (TBPH), triphenyl phosphate (TPP), and isopropylated triphenyl phosphate (IPTP). Animal and in vitro studies suggest that FM550, TPP and IPTP may have adipogenic effects and may exert these effects through PPARγ activation. Using murine 3T3-L1 preadipocytes, we investigated the detailed expression of transcription factors and adipogenic markers in response to FM550 and its components...
2017: PloS One
https://www.readbyqxmd.com/read/28426713/metallothioneins-regulate-the-adipogenic-differentiation-of-3t3-l1-cells-via-the-insulin-signaling-pathway
#15
Yoshito Kadota, Yuriko Toriuchi, Yuka Aki, Yuto Mizuno, Takashige Kawakami, Tomoko Nakaya, Masao Sato, Shinya Suzuki
Knockout of metallothionein (MT) genes contributes to a heavier body weight in early life and the potential to become obese through the intake of a high fat diet (HFD) in mice. It has thus been suggested that MT genes regulate the formation of adipose tissue, which would become the base for later HFD-induced obesity. We evaluated the fat pads of mice during the lactation stage. The fat mass and adipocyte size of MT1 and MT2 knockout mice were greater than those of wild type mice. Next, we assayed the ability of small interfering RNA (siRNA) to silence MT genes in the 3T3-L1 cell line...
2017: PloS One
https://www.readbyqxmd.com/read/28420612/identification-and-analysis-of-zfpm2-as-a-target-gene-of-mir-17-92-cluster-in-chicken
#16
Zhang Xiaofei, Song He, Liu Jing, Zhang Wenjian, Yan Xiaohong, Li Hui, Wang Ning
The miR-17-92 cluster plays important roles in a variety of physiological and pathological processes in mammals. Previously, we showed that miR-17-92 cluster promotes chicken preadipocyte proliferation; however, the mechanism for its action is unknown. In order to explore the mechanism by which miR-17-92 cluster promotes chicken preadipocyte proliferation, CCK8 proliferation assay was performed to determine the effect of ZFPM2 knockdown on chicken preadipocyte proliferation. The results showed that ZFPM2 knockdown significantly promoted chicken preadipocyte proliferation (P<0...
April 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28417922/indole-derivatives-isolated-from-brown-alga-sargassum-thunbergii-inhibit-adipogenesis-through-ampk-activation-in-3t3-l1-preadipocytes
#17
Min-Cheol Kang, Yuling Ding, Eun-A Kim, Youn Kyung Choi, Thiago de Araujo, Soo-Jin Heo, Seung-Hong Lee
Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives (STCs)-indole-2-carboxaldehyde (STC-1), indole-3-carboxaldehyde (STC-2), indole-4-carboxaldehyde (STC-3), indole-5-carboxaldehyde (STC-4), indole-6-carboxaldehyde (STC-5), and indole-7-carboxaldehyde (STC-6)-from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells...
April 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28398659/hypaphorine-an-indole-alkaloid-isolated-from-caragana-korshinskii-kom-inhibites-3t3-l1-adipocyte-differentiation-and-improves-insulin-sensitivity-in-vitro
#18
Guangxiang Luan, Fangfang Tie, Zhenzhen Yuan, Gang Li, Jie He, Zhenhua Wang, Honglun Wang
Obesity, a major health problem worldwide, is a complex multifactorial chronic disease that increases the risk for insulin resistance, type 2 diabetes, coronary heart disease and hypertension. In this study, we assessed methods to isolate hypaphorine, a potent drug candidate for obesity and insulin resistance. Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) was established as a method to separate three compounds, adenosine, L-tryptophan and hypaphorine, from the crude extracts of Caragana korshinskii Kom...
April 11, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28393240/do-in-seung-gi%C3%A2-tang-extract-suppresses-adipocyte-differentiation-in-3t3%C3%A2-l1-cells
#19
Eun Hyoung Shim, Myeong Soo Lee, Ju-Ah Lee, Hoyoung Lee
Blood stasis syndrome (BSS), additionally called Eohyul, is a basic pathological concept in Traditional Korean Medicine. Do In Seung Gi‑Tang (DISGT) is herbal medicine used for the treatment of BSS. It primarily treats metabolic diseases (MDs) including obesity, hypertension, diabetes mellitus and gynecological diseases, by promoting blood circulation. The present study aimed to investigate the anti‑adipogenesis effect of DISGT in 3T3‑L1 adipocytes using Oil Red O staining, and assessing levels of triglycerides (TGs) and leptin in adipocytes by ELISA and western blot analysis...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28351837/cyclin-c-regulates-adipogenesis-by-stimulating-transcriptional-activity-of-ccaat-enhancer-binding-protein-alpha
#20
Ziyi Song, Alus M Xiaoli, Quanwei Zhang, Yi Zhang, Ellen S T Yang, Sven Wang, Rui Chang, Zhengdong D Zhang, Gongshe Yang, Randy Strich, Jeffrey E Pessin, Fajun Yang
Brown adipose tissue (BAT) is important for maintaining energy homeostasis and adaptive thermogenesis in rodents and humans. As disorders arising from dysregulated energy metabolism, such as obesity and metabolic diseases, have increased, so has interest in the molecular mechanisms in adipocyte biology. Using a functional screen, we identified cyclin C (CycC), a conserved subunit of the Mediator complex, as a novel regulator for brown adipocyte formation. siRNA-mediated CycC knockdown (KD) in brown preadipocytes impaired the early transcriptional program of differentiation, and genetic knockout (KO) of CycC completely blocked the differentiation process...
March 28, 2017: Journal of Biological Chemistry
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