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preadipocyte AND proliferation

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https://www.readbyqxmd.com/read/27914036/prolactin-regulatory-element-binding-protein-is-involved-in-suppression-of-the-adiponectin-gene-in-vivo
#1
X Z Zhang, H Imachi, J Y Lyu, K Fukunaga, S Sato, T Ibata, T Kobayashi, T Yoshimoto, F Kikuchi, T Dong, K Murao
PURPOSE: Prolactin regulatory element-binding protein (PREB), a member of the WD-repeat protein family, has been recognized as a transcriptional factor that regulates prolactin promoter activity in the anterior pituitary of rats. PREB is expressed not only in the pituitary but also in various other tissues, including the adipose tissue. Previous studies have shown that PREB acts as a transcriptional regulator and suppresses the expression of the adiponectin gene in cultured 3T3L1 preadipocytes...
December 2, 2016: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/27906176/epigenetic-programming-of-dnmt3a-mediated-by-ap2%C3%AE-is-required-for-granting-preadipocyte-the-ability-to-differentiate
#2
Wei Guo, Jiangnan Chen, Ying Yang, Jianbei Zhu, Jiarui Wu
Adipogenesis has an important role in regulating energy homeostasis in mammals. 3T3-L1 preadipocytes have been widely used as an in vitro model for analyzing the molecular mechanism of adipogenesis. Previous reports indicated that the stage of contact inhibition (CI), through which the proliferating cells exit from the cell cycle, was required for granting preadipocyte the ability to differentiate. While this kind of the granting mechanism remains elusive. In the present study, we showed that DNA (cytosine-5) methyltransferase 3a (Dnmt3a) was upregulated at both the mRNA and protein level during the CI stage, and resulted in increasing promoter methylation of adipogenic genes...
December 1, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27899593/the-kdm5-family-is-required-for-activation-of-pro-proliferative-cell-cycle-genes-during-adipocyte-differentiation
#3
Ann-Sofie B Brier, Anne Loft, Jesper G S Madsen, Thomas Rosengren, Ronni Nielsen, Søren F Schmidt, Zongzhi Liu, Qin Yan, Hinrich Gronemeyer, Susanne Mandrup
The KDM5 family of histone demethylases removes the H3K4 tri-methylation (H3K4me3) mark frequently found at promoter regions of actively transcribed genes and is therefore generally considered to contribute to corepression. In this study, we show that knockdown (KD) of all expressed members of the KDM5 family in white and brown preadipocytes leads to deregulated gene expression and blocks differentiation to mature adipocytes. KDM5 KD leads to a considerable increase in H3K4me3 at promoter regions; however, these changes in H3K4me3 have a limited effect on gene expression per se...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27888346/methionine-and-cysteine-deficiencies-altered-proliferation-rate-and-time-course-differentiation-of-porcine-preadipose-cells
#4
Rosa Castellano, Marie-Hélène Perruchot, Sophie Tesseraud, Sonia Métayer-Coustard, Elizabeth Baeza, Yves Mercier, Florence Gondret
Methionine (Met) is an essential sulfur amino acid (AA) limiting growth and is the precursor of cysteine (Cys), the rate-limiting factor in the synthesis of glutathione, and the main intracellular non-enzymatic antioxidant. This study aimed at determining the effects of limited supplies in Met and(or) Cys in early aspects of adipose tissue development and oxidative stress in differentiated adipocytes. Incremental reductions in Met (70, 40, and 0 µM) were compared with Met 100 µM (control dose) in porcine preadipocytes cultured in media without or with Cys (250 µM)...
November 25, 2016: Amino Acids
https://www.readbyqxmd.com/read/27884413/peroxisome-proliferator-activated-receptor-%C3%AE-down-regulation-mediates-the-inhibitory-effect-of-d-%C3%AE-tocotrienol-on-the-differentiation-of-murine-3t3-f442a-preadipocytes
#5
Sheida Torabi, Hoda Yeganehjoo, Chwan-Li Shen, Huanbiao Mo
Tocotrienols accelerate the degradation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase that catalyzes the biosynthesis of mevalonate; the latter is essential for preadipocyte differentiation. Tocotrienols also down-regulate peroxisome proliferator-activated receptor γ (PPARγ), a key regulator of adipocyte differentiation. We hypothesized that mevalonate deprivation and PPARγ down-regulation mediate d-δ-tocotrienol-induced inhibition of adipocyte differentiation. The objectives of this study were to determine the effect of d-δ-tocotrienol on 3T3-F442A preadipocyte differentiation and the involvement of PPARγ and mevalonate...
November 3, 2016: Nutrition Research
https://www.readbyqxmd.com/read/27869309/roles-of-notch-signaling-in-adipocyte-progenitor-cells-and-mature-adipocytes
#6
REVIEW
Tizhong Shan, Jiaqi Liu, Weiche Wu, Ziye Xu, Yizhen Wang
Adipose tissues, composed with mature adipocytes and preadipocytic stromal/stem cells, play crucial roles in whole body energy metabolism and regenerative medicine. Mature adipocytes are derived and differentiated from mesenchymal stem cells (MSCs) or preadipocytes. This differentiation process, also called adipogenesis, is regulated by several signaling pathways and transcription factors. Notch1 signaling is a highly conserved pathway that is indispensable for stem cell hemostasis and tissue development. In adipocyte progenitor cells, Notch1 signaling regulates the adipogenesis process including proliferation and differentiation of the adipocyte progenitor cells in vitro...
November 21, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27867269/platyphylloside-isolated-from-betula-platyphylla-inhibit-adipocyte-differentiation-and-induce-lipolysis-via-regulating-adipokines-including-ppar%C3%AE-in-3t3-l1-cells
#7
Mina Lee, Sang Hyun Sung
BACKGROUND: Obesity causes or aggravates many health problems, both independently and in association with several pathological disorders, including Type II diabetes, hypertension, atherosclerosis, and cancer. Therefore, we screened small compounds isolated from natural products for the development of anti-obesity drugs. OBJECTIVE: The purpose of this study was to investigate the anti-adipogenic activities of platyphylloside, diarylheptanoid isolated from Betula platyphylla, which was selected based on the screening using 3T3-L1 cells...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27846311/cold-exposure-induces-proliferation-of-mature-brown-adipocyte-in-a-%C3%A3-3-adrenergic-receptor-mediated-pathway
#8
Keigo Fukano, Yuko Okamatsu-Ogura, Ayumi Tsubota, Junko Nio-Kobayashi, Kazuhiro Kimura
Hyperplasia of brown adipose tissue (BAT) is a fundamental mechanism for adaptation to survive in the cold environment in rodents. To determine which cell types comprising BAT contribute to tissue hyperplasia, immunohistochemical analysis using a proliferative marker Ki67 was performed on the BAT from 6-week-old C57BL/6J mice housed at 23°C (control) or 10°C (cold) for 5 days. Interestingly, in the control group, the cell proliferative marker Ki67 was detected in the nuclei of uncoupling protein 1-positive mature brown adipocytes (7...
2016: PloS One
https://www.readbyqxmd.com/read/27830366/maturation-of-adipocytes-is-suppressed-by-fluid-shear-stress
#9
Jongyun Choi, Sei Young Lee, Yeong-Min Yoo, Chi Hyun Kim
Preadipocytes are mechano-responsive cells and their differentiation to adipocytes may be regulated by various types of physical stimulation. Understanding the mechanism of differentiation, which increases the number of adipocytes and lipid accumulation is important in the study of obesity-related diseases. In this study, we investigated the effects of physical stimulation at different stages of adipogenic differentiation using physiological levels of fluid shear stress. Preadipocytes were treated with dexamethasone, 3-isobutyl-1-methylxanthine and insulin for 3 days (induction period) and incubated for additional 6 days for maturation...
November 9, 2016: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27825360/a-possible-regulatory-link-between-twist-1-and-ppar%C3%AE-gene-regulation-in-3t3-l1-adipocytes
#10
Rui Ren, Zhufeng Chen, Xia Zhao, Tao Sun, Yuchao Zhang, Jie Chen, Sumei Lu, Wanshan Ma
BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a critical gene that regulates the function of adipocytes. Therefore, studies on the molecular regulation mechanism of PPARγ are important to understand the function of adipose tissue. Twist 1 is another important functional gene in adipose tissue, and hundreds of genes are regulated by Twist 1. The aim of this study was to investigate the regulation of Twist 1 and PPARγ expression in 3T3-L1 mature adipocytes. METHODS: We induced differentiation in 3T3-L1 preadipocytes and examined alterations in Twist 1 and PPARγ expression...
November 8, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27803314/promotion-of-adipogenesis-by-neuropeptide-y-during-the-later-stages-of-chicken-preadipocyte-differentiation
#11
Steven L Shipp, Mark A Cline, Elizabeth R Gilbert
Neuropeptide Y (NPY) promotes adipogenesis in both birds and mammals, although mechanisms in avians remain unclear. The objective of this study was thus to evaluate effects of NPY on chick preadipocyte proliferation and differentiation. Preadipocytes were treated with 0, 1, 10, or 100 nmol/L NPY and gene expression and cellular proliferation were evaluated at 12, 24, and 48 h. At 12 h posttreatment, mRNA abundance of topoisomerase II alpha (TOP2A), and thioredoxin-dependent peroxidase 2 was upregulated and NPY was downregulated in response to NPY (0 vs...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27780975/the-role-and-possible-mechanism-of-lncrna-u90926-in-modulating-3t3-l1-preadipocyte-differentiation
#12
J Chen, Y Liu, S Lu, L Yin, C Zong, S Cui, D Qin, Y Yang, Q Guan, X Li, X Wang
BACKGROUND: Obesity is a risk factor for metabolic diseases, while preadipocyte differentiation or adipogenesis is closely related to obesity occurrence. Long noncoding RNAs (lncRNAs) are a unique class of transcripts in regulation of a variety of biological processes. Using cDNA microarray, we found lncRNA U90926 is negatively correlated with 3T3-L1 preadipocyte differentiation. OBJECTIVE: The aim of this study was to explore the role of lncRNA U90926 (lnc-U90926) in adipogenesis and the underlying mechanisms...
October 26, 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27777595/soshiho-tang-aqueous-extract-exerts-antiobesity-effects-in-high-fat-diet-fed-mice-and-inhibits-adipogenesis-in-3t3-l1-adipocytes
#13
Sae-Rom Yoo, Mee-Young Lee, Byoung-Kab Kang, Hyeun-Kyoo Shin, Soo-Jin Jeong
Soshiho-tang (SST; sho-saiko-to in Japanese; xiaochaihu-tang in Chinese) has generally been used to improve liver fibrosis- and cirrhosis-related symptoms in traditional Korean medicine. Although many studies have investigated the pharmacological properties of SST, its antiobesity effect has not been elucidated. Thus, our present study was carried out to evaluate the antiobesity effect of SST using a high fat diet- (HFD) induced obese mouse model and 3T3-L1 adipose cells. C57BL/6J mice were randomly divided into four groups (n = 6/group), normal diet (ND), HFD-fed group, and HFD- and SST-fed groups (S200: 200 mg/kg of SST; S600: 600 mg/kg of SST) and given HFD with or without SST extract for 8 weeks...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27769066/elevation-of-n-3-n-6-pufas-ratio-suppresses-mtorc1-and-prevents-colorectal-carcinogenesis-associated-with-apc-mutation
#14
Miao Liu, Ling Zhou, Baiyu Zhang, Minhong He, Xiaoying Dong, Xiaojun Lin, Chunhong Jia, Xiaochun Bai, Yifan Dai, Yongchun Su, Zhipeng Zou, Hang Zheng
Although epidemiological and preclinical studies have shown the preventative effect of n-3 polyunsaturated fatty acids (PUFAs) on colorectal cancer (CRC), the underlying molecular mechanisms are not clear. In this study, we revealed that elevation of n-3/n-6 PUFAs ratio suppress the mechanistic target of rapamycin complex 1 (mTORC1) and prevent colorectal tumorigenesis. The transgenic expression of fat-1, a desaturase that catalyzes the conversion of n-6 to n-3 PUFAs and produces n-3 PUFAs endogenously, repressed colorectal tumor cell growth and remarkably reduced tumor burden, and alleviated anemia as well as hyperlipidemia in APCMin/+ (adenomatous polyposis coli) mice, a classic CRC model that best simulates most clinical cases...
October 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27753270/obesity-modifies-bone-marrow-microenvironment-and-directs-bone-marrow-mesenchymal-cells-to-adipogenesis
#15
Simone Vargas da Silva, Mariana Renovato-Martins, Cristiane Ribeiro-Pereira, Marta Citelli, Christina Barja-Fidalgo
OBJECTIVE: To investigate the role of obesity on the bone marrow microenvironment and evaluate its possible impact on the adipogenic potential of mesenchymal stem cells (MSC). METHODS: C57BL/6 male mice were fed with a high-fat diet (HFD) for 10 weeks. Femurs and tibiae were collected, and bone marrow mesenchymal stem cells (BM-MSC) were isolated and analyzed for proliferative potential, immunophenotype, and expression of adipogenesis markers. Their capacity to produce extracellular matrix proteins and proinflammatory cytokines in vitro was also evaluated...
October 18, 2016: Obesity
https://www.readbyqxmd.com/read/27742678/mir-181a-5p-regulates-3t3-l1-cell-adipogenesis-by-targeting-smad7-and-tcf7l2
#16
Dan Ouyang, Lifeng Xu, Lihua Zhang, Dongguang Guo, Xiaotong Tan, Xiaofang Yu, Junjie Qi, Yaqiong Ye, Qihong Liu, Yongjiang Ma, Yugu Li
MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation...
October 14, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27717825/%C3%AE-msh-promotes-preadipocyte-proliferation-by-alleviating-er-stress-induced-leptin-resistance-and-by-activating-notch1-signal-in-mice
#17
Lu Gan, Zhenjiang Liu, Tianjiao Wu, Fei Feng, Chao Sun
Alpha-melanocyte stimulating hormone (αMSH) has an important role in the regulation of body weight and energy expenditure. Nevertheless, the molecular mechanisms of circulating αMSH on preadipocyte proliferation remain elusive. We found αMSH was reduced by high fat diet (HFD) while leptin was elevated in adipose tissue. Leptin resistance and endoplasmic reticulum (ER) stress of adipose tissue were increased in obese mice. αMSH increased leptin sensitivity and alleviated ER stress along with increased p-STAT3 level and reduced SOCS3, GRP78, CHOP, ATF4, p27 and p53 levels...
October 5, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27702590/annexin-a6-regulates-adipocyte-lipid-storage-and-adiponectin-release
#18
Sabrina Krautbauer, Elisabeth M Haberl, Kristina Eisinger, Rebekka Pohl, Lisa Rein-Fischboeck, Carles Rentero, Anna Alvarez-Guaita, Carlos Enrich, Thomas Grewal, Christa Buechler, Markus Neumeier
Lipid storage and adipokine secretion are critical features of adipocytes. Annexin A6 (AnxA6) is a lipid-binding protein regulating secretory pathways and its role in adiponectin release was examined. The siRNA-mediated AnxA6 knock-down in 3T3-L1 preadipocytes impaired proliferation, and differentiation of AnxA6-depleted cells to mature adipocytes was associated with higher soluble adiponectin and increased triglyceride storage. The latter was partly attributed to reduced lipolysis. Accordingly, AnxA6 overexpression in 3T3-L1 adipocytes lowered cellular triglycerides and adiponectin secretion...
October 1, 2016: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/27685706/sfrp2-is-associated-with-increased-adiposity-and-vegf-expression
#19
Rachel K Crowley, Michael W O'Reilly, Iwona J Bujalska, Zaki K Hassan-Smith, Jonathan M Hazlehurst, Danielle R Foucault, Paul M Stewart, Jeremy W Tomlinson
AIMS: The aim of this study was to assess depot-specific expression and secretion of secreted frizzled-related protein 2 (sFRP2) by adipose tissue and its effect on adipocyte biology. We measured serum sFRP2 concentrations in 106 patients in vivo to explore its relationship to fat mass, glycaemia and insulin resistance. METHODS: Expression of sFRP2 in mouse and human tissues was assessed using polymerase chain reaction and Western blot. Western blot confirmed secretion of sFRP2 by adipose tissue into cell culture medium...
2016: PloS One
https://www.readbyqxmd.com/read/27669202/potential-of-natural-products-in-the-inhibition-of-adipogenesis-through-regulation-of-ppar%C3%AE-expression-and-or-its-transcriptional-activity
#20
REVIEW
Shi Feng, Laura Reuss, Yu Wang
Obesity is a global health problem characterized as an increase in the mass of adipose tissue. Adipogenesis is one of the key pathways that increases the mass of adipose tissue, by which preadipocytes mature into adipocytes through cell differentiation. Peroxisome proliferator-activated receptor γ (PPARγ), the chief regulator of adipogenesis, has been acutely investigated as a molecular target for natural products in the development of anti-obesity treatments. In this review, the regulation of PPARγ expression by natural products through inhibition of CCAAT/enhancer-binding protein β (C/EBPβ) and the farnesoid X receptor (FXR), increased expression of GATA-2 and GATA-3 and activation of the Wnt/β-catenin pathway were analyzed...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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