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preadipocyte AND proliferation

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https://www.readbyqxmd.com/read/29663555/dehydroleucodine-inhibits-mitotic-clonal-expansion-during-adipogenesis-through-cell-cycle-arrest
#1
S Abood, M L Veisaga, L A López, M A Barbieri
Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner...
April 16, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29611347/activation-of-the-sympathetic-nervous-system-suppresses-mouse-white-adipose-tissue-hyperplasia-through-the-%C3%AE-1-adrenergic-receptor
#2
Mary K Schneider, Bingzhong Xue, Hang Shi
Adipose tissue (AT) expands via both hypertrophy and hyperplasia during the development of obesity. While AT hypertrophy involves the increase in size of existing adipocytes, hyperplasia is the process of creating new adipocytes from the pool of adipocyte precursor cells (APCs), which includes adipocyte progenitor cells and preadipocytes. Prior studies have implicated a role of the sympathetic nervous system (SNS) in regulation of hyperplasia in white adipose tissue (WAT). Here, we aimed to determine the mechanisms underlying SNS regulation of APC proliferation in mouse WAT...
April 2018: Physiological Reports
https://www.readbyqxmd.com/read/29606113/md-1-a-poly-herbal-formulation-indicated-in-diabetes-mellitus-ameliorates-glucose-uptake-and-inhibits-adipogenesis-an-in-vitro-study
#3
Srivani Telapolu, Mangathayaru Kalachavedu, Alan Mathew Punnoose, Dwarakanath Bilikere
BACKGROUND: Type 2 Diabetes (T2D) is a polygenic disease requiring a multipronged therapeutic approach. In the current scenario, the use of polyherbals is increasing among the diabetics. MD-1, a poly herbal formulation is constituted as a mixture of six popular anti diabetic herbs, used in the management of Diabetes mellitus (DM). The physicochemical, biochemical and in vitro efficacy studies have been carried out to ascertain the possible mechanisms underlying the anti-diabetic action of MD-1...
April 2, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29604586/inhibitory-effect-of-sinigrin-on-adipocyte-differentiation-in-3t3-l1-cells-involvement-of-ampk-and-mapk-pathways
#4
Hee-Weon Lee, Dong-Kwon Rhee, Byung-Oh Kim, Suhkneung Pyo
Adipocyte differentiation is a critical adaptive response to nutritional overload and affects the metabolic outcome of obesity. Sinigrin (2-propenyl glucosinolate) is a glucosinolate belong to the glucoside contained in broccoli, brussels sprouts, and black mustard seeds. We investigated the effects of sinigrin on adipogenesis in 3T3-L1 preadipocytes and its underlying mechanisms. Sinigrin remarkably inhibited the accumulation of lipid droplets and adipogenesis by downregulating the expression of CCAAT-enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), leptin and aP2...
March 28, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29604463/identification-of-differentially-expressed-micrornas-during-preadipocyte-differentiation-in-chinese-crested-duck
#5
Shasha Wang, Yang Zhang, Xiaoya Yuan, Rui Pan, Wencheng Yao, Li Zhong, Qianqian Song, Shenghan Zheng, Zhixiu Wang, Qi Xu, Guobin Chang, Guohong Chen
MicroRNAs (miRNAs) are considered key players in the regulation of a broad range of biological processes. Specifically, miRNAs have been reported to play an important role in the process of adipogenesis. In this study, we constructed a model of adipogenesis by isolating preadipocytes (WCC) derived from adipose tissue and preadipocytes after 72 h differentiation (WCT) in vitro. Deep sequencing of miRNAs expressed in WCT and WCC cells was conducted; we identified 105 differentially expressed miRNA candidates (fifty up-regulated and fifty-five down-regulated)...
March 28, 2018: Gene
https://www.readbyqxmd.com/read/29589999/biguanides-metformin-and-phenformin-generate-therapeutic-effects-via-amp-activated-protein-kinase-extracellular-regulated-kinase-pathways-in-an-in-vitro-model-of-graves-orbitopathy
#6
Ye Eon Han, Sena Hwang, Jin Hee Kim, Jung Woo Byun, Jin Sook Yoon, Eun Jig Lee
BACKGROUND: It was hypothesized that the biguanides metformin and phenformin, which are anti-hyperglycemic drugs used for diabetes mellitus, would have therapeutic effects in an in vitro model of Graves' orbitopathy (GO). Because adipogenesis, hyaluronan production, and inflammation are considered important in the pathogenesis of GO, this study aimed to determine the therapeutic effects and underlying mechanisms of biguanides on these parameters. METHODS: In vitro experiments were performed using primary cultured orbital fibroblasts from patients with GO...
April 2018: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/29545541/toosendanin-inhibits-adipogenesis-by-activating-wnt-%C3%AE-catenin-signaling
#7
Tian-Xing Chen, Xiao-Ying Cheng, Yun Wang, Wu Yin
Toosendanin (TSN), a triterpenoid extracted from Melia toosendan, has been reported to possess anti-oxidant, anti-inflammatory, anti-allergic, and anti-arthritic activities. However, its anti-adipogenic effect remains unknown. Here, we found that TSN dose-dependently attenuated lipid accumulation in preadipocytes 3T3-L1 as evidenced by Oil Red O staining. TSN also significantly downregulated mRNA and protein levels of adipocytokines (adiponectin and leptin), CCAAT/enhancer binding proteins α (C/EBP-α), peroxisome proliferator-activated receptor γ (PPAR-γ), fatty acid synthase, and acetyl-CoA carboxylase in adipocytes...
March 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29535768/neuropilin-1-mediates-keratinocyte-growth-factor-signaling-in-adipose-derived-stem-cells-potential-involvement-in-adipogenesis
#8
Simona Ceccarelli, Cristina Nodale, Enrica Vescarelli, Paola Pontecorvi, Valeria Manganelli, Giovanni Casella, Maria Giuseppina Onesti, Maurizio Sorice, Ferdinando Romano, Antonio Angeloni, Cinzia Marchese
Adipogenesis is regulated by a complex network of molecules, including fibroblast growth factors. Keratinocyte growth factor (KGF) has been previously reported to promote proliferation on rat preadipocytes, although the expression of its specific receptor, FGFR2-IIIb/KGFR, is not actually detected in mesenchymal cells. Here, we demonstrate that human adipose-derived stem cells (ASCs) show increased expression of KGF during adipogenic differentiation, especially in the early steps. Moreover, KGF is able to induce transient activation of ERK and p38 MAPK pathways in these cells...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29514876/adipogenesis-in-fish
#9
REVIEW
Cristina Salmerón
White adipose tissue (AT) is the main lipid storage depot in vertebrates. Initially considered to be a simple lipid store, AT has recently been recognized as playing a role as an endocrine organ that is implicated in processes such as energy homeostasis and as a rich source of stem cells. Interest in adipogenesis has increased not only because of the prevalence of obesity, metabolic syndrome and type 2 diabetes in humans, but also in aquaculture because of the excessive fat deposition experienced in some cultured fish species, which may compromise both their welfare and their final product quality...
March 7, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29501772/antihyperlipidemic-effect-of-a-rhamnus-alaternus-leaf-extract-in-triton-induced-hyperlipidemic-rats-and-human-hepg2-cells
#10
Mustapha Tacherfiout, Petar D Petrov, Marco Mattonai, Erika Ribechini, Joan Ribot, M Luisa Bonet, Bachra Khettal
The Mediterranean buckthorn, Rhamnus alaternus L., is a plant used in traditional medicine in Mediterranean countries. We aimed at characterizing its phenolic compounds and explore potential antihyperlipidemic activity of this plant. The profile of phenolic compounds in R. alaternus leaf crude methanolic extract (CME) and its liquid-liquid extraction-derived fractions were analyzed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS2 ). Effects of CME on: circulating lipids in rats with Triton WR-1339-induced hyperlipidemia, intracellular lipid accumulation and expression of genes of fatty acid metabolism in human hepatoma HepG2 cells, and adipogenesis in the 3T3-L1 murine adipocyte cell model were assessed...
March 1, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29501496/stereoselective-effects-of-lactate-enantiomers-on-the-enhancement-of-3t3-l1-adipocyte-differentiation
#11
Naoki Harada, Ito Hirano, Hiroshi Inui, Ryoichi Yamaji
Lactate contains a chiral carbon and thus has two optical isomers-d-lactate and l-lactate. l-Lactate is the predominant form that is produced by the body and can be delivered to the organs. On the other hand, gut microbiota produce both isomers, which can then flow into the body. Although both d-lactate and l-lactate can contribute to energy metabolism, their potential in adipocyte differentiation remain to be elucidated. Here, we investigated the effects of l-lactate and d-lactate on the differentiation of 3T3-L1 preadipocytes...
February 28, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29493813/rapid-adipose-tissue-expansion-triggers-unique-proliferation-and-lipid-accumulation-profiles-in-adipose-tissue-macrophages
#12
Lindsey A Muir, Samadhi Kiridena, Cameron Griffin, Jennifer B DelProposto, Lynn Geletka, Gabriel Martinez-Santibañez, Brian F Zamarron, Hannah Lucas, Kanakadurga Singer, Robert W O' Rourke, Carey N Lumeng
Obesity-related changes in adipose tissue leukocytes, in particular adipose tissue macrophages (ATMs) and dendritic cells (ATDCs), are implicated in metabolic inflammation, insulin resistance, and altered regulation of adipocyte function. We evaluated stromal cell and white adipose tissue (WAT) expansion dynamics with high fat diet (HFD) feeding for 3-56 days, quantifying ATMs, ATDCs, endothelial cells (ECs), and preadipocytes (PAs) in visceral epididymal WAT and subcutaneous inguinal WAT. To better understand mechanisms of the early response to obesity, we evaluated ATM proliferation and lipid accumulation...
March 1, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29486135/transcriptional-regulation-of-tcf-lef-and-ppar%C3%AE-by-daidzein-and-genistein-in-3t3-l1-preadipocytes
#13
Jeremy E Davis, Darcie Hastings
Adipogenesis is central to adipose tissue plasticity and lipid homeostasis. Regulation of adipogenic signaling by phytoestrogens has been implicated as a potential therapeutic strategy for obesity-related disease. However, it remains unclear how these compounds directly impact transcriptional control of adipogenesis. As such, the focus of this study was to determine how daidzein and genistein effect transcriptional activation of peroxisome proliferator-activated receptor gamma (PPARγ) and TCF/LEF in 3T3-L1 preadipocytes...
February 27, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29484393/spontaneous-adipogenic-differentiation-potential-of-adipose%C3%A2-derived-stem-cells-decreased-with-increasing-cell-passages
#14
Duo Yang, Na Li, Guoying Zhang
Primary adipose-derived stem cells (ADSCs) are a mixture of cell types including preadipocytes having the ability to spontaneously differentiate into adipocytes. The aim of the present study was to compare the spontaneous adipogenic differentiation potential of ADSCs at different passages to determine whether it decreased with continuous cell passages. Mouse ADSCs (mADSCs) were harvested and cells from passages 1 to 5 were used for experiments. The proliferation of mADSCs at different passages was tested using the cell counting kit‑8 assay...
February 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29483204/mir-103-107-promote-er-stress-mediated-apoptosis-via-targeting-the-wnt3a-%C3%AE-catenin-atf6-pathway-in-preadipocytes
#15
Zhenzhen Zhang, Song Wu, Saeed Muhammad, Qian Ren, Chao Sun
Both miR-103 and 107 have been demonstrated to restrain cell proliferation, regulate lipid metabolism and inflammation. However, the effects of miR-103/107 on preadipocytes apoptosis remain unknown. In the present research, we have investigated how miR-103/107 regulated preadipocytes apoptosis. We found that miR-103/107 aggravated Endoplasmic Reticulum (ER) stress mediated apoptosis in preadipocytes. We confirmed that miR-103/107 targeted Wnt3a (WNT family member 3a) in preadipocytes. It was found that overexpressing Wnt3a resulted in suppression of ER stress mediated apoptosis, while restoration of miR-103/107 counteracted the effects of Wnt3a in preadipocytes...
February 26, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29474931/alpha-syntrophin-null-mice-are-protected-from-non-alcoholic-steatohepatitis-in-the-methionine-choline-deficient-diet-model-but-not-the-atherogenic-diet-model
#16
Lisa Rein-Fischboeck, Elisabeth M Haberl, Rebekka Pohl, Verena Schmid, Susanne Feder, Sabrina Krautbauer, Gerhard Liebisch, Christa Buechler
Adipose tissue dysfunction contributes to the pathogenesis of non-alcoholic steatohepatitis (NASH). The adapter protein alpha-syntrophin (SNTA) is expressed in adipocytes. Knock-down of SNTA increases preadipocyte proliferation and formation of small lipid droplets, which are both characteristics of healthy adipose tissue. To elucidate a potential protective role of SNTA in NASH, SNTA null mice were fed a methionine-choline-deficient (MCD) diet or an atherogenic diet which are widely used as preclinical NASH models...
February 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29441719/azd1208-a-pan-pim-kinase-inhibitor-inhibits-adipogenesis-and-induces-lipolysis-in-3t3-l1-adipocytes
#17
Yu-Kyoung Park, Brice Wilfried Obiang-Obounou, Kyung-Bok Lee, Jong-Soon Choi, Byeong-Churl Jang
The proviral integration moloney murine leukaemia virus (Pim) kinases, consisting of Pim-1, Pim-2 and Pim-3, are involved in the control of cell growth, metabolism and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. AZD1208 is a pan-Pim kinase inhibitor and is known for its anti-cancer activity. In this study, we investigated the effect of AZD1208 on adipogenesis and lipolysis in 3T3-L1 cells, a murine preadipocyte cell line. AZD1208 markedly suppressed lipid accumulation and reduced triglyceride contents in differentiating 3T3-L1 cells, suggesting the drug's anti-adipogenic effect...
February 14, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29414921/microrna-125a-5p-affects-adipocytes-proliferation-differentiation-and-fatty-acid-composition-of-porcine-intramuscular-fat
#18
Jingjing Du, Yan Xu, Peiwen Zhang, Xue Zhao, Mailin Gan, Qiang Li, Jideng Ma, Guoqing Tang, Yanzhi Jiang, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipogenesis. Previously, microRNA-125a-5p (miR-125a-5p) was identified as an important regulator of adipogenesis. In the present study, we found that the expression of miR-125a-5p is dynamically regulated during porcine intramuscular preadipocytes differentiation and that its expression levels in different porcine muscle tissues were negatively involved with IMF content...
February 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29410257/plasticizers-used-in-food-contact-materials-affect-adipogenesis-in-3t3-l1-cells
#19
Valentina Pomatto, Erika Cottone, Paolo Cocci, Matteo Mozzicafreddo, Gilberto Mosconi, Erik Russel Nelson, Francesco Alessandro Palermo, Patrizia Bovolin
Recent studies suggest that exposure to some plasticizers, such as Bisphenol A (BPA), play a role in endocrine/metabolic dispruption and can affect lipid accumulation in adipocytes. Here, we investigated the adipogenic activity and nuclear receptor interactions of four plasticizers approved for the manufacturing of food-contact materials (FCMs) and currently considered safer alternatives. Differentiating 3T3-L1 mouse preadipocytes were exposed to scalar concentrations (0.01-25 μM) of DiNP (Di-iso-nonyl-phthalate), DiDP (Di-iso-decyl-phthalate), DEGDB (Diethylene glycol dibenzoate), or TMCP (Tri-m-cresyl phosphate)...
February 2, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29396442/nonthermal-plasma-treated-solution-inhibits-adipocyte-differentiation-and-lipogenesis-in-3t3-l1-preadipocytes-via-er-stress-signal-suppression
#20
Sung Un Kang, Haeng Jun Kim, Dae Ho Kim, Chang Hak Han, Yun Sang Lee, Chul-Ho Kim
The accumulation and differentiation of adipocytes contribute to the development of obesity and metabolic diseases. It is well-known that interactions of transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and endoplasmic reticulum (ER) stress are required for adipogenesis. Recently, use of nonthermal atmospheric plasma (NTP) is expanding from the biomedical field into various other fields. In this study, we investigated whether nonthermal plasma-treated solution (NTS) has an inhibitory effect on adipogenesis and elucidated its mechanisms...
February 2, 2018: Scientific Reports
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