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https://www.readbyqxmd.com/read/29310789/investigating-cell-type-specific-functions-of-tenascin-c
#1
Devadarssen Murdamoothoo, Anja Schwenzer, Jessica Kant, Tristan Rupp, Anna Marzeda, Kim Midwood, Gertraud Orend
The extracellular matrix molecule tenascin-C (TNC) has received a lot of attention since its discovery 30 years ago because of its multiple roles in tissue repair, and in pathologies such as chronic inflammation, fibrosis, and cancer. Mouse models with high or no TNC expression have enabled the validation of key roles for TNC in immunity and angiogenesis. In parallel, many approaches including primary cell or organ cultures have shed light on the cellular and molecular mechanisms by which TNC exerts its multiple actions in vivo...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29296225/significance-of-the-e3-ubiquitin-protein-ubr5-as-an-oncogene-and-a-prognostic-biomarker-in-colorectal-cancer
#2
Zhongdong Xie, Han Liang, Jinmeng Wang, Xiaowen Xu, Yan Zhu, Aizhen Guo, Xian Shen, Fuao Cao, Wenjun Chang
The E3 ubiquitin protein UBR5 has been implicated in the regulation of multiple biological functions and has recently emerged as a key regulator of the ubiquitin-proteasome system (UPS) in cancer. However, the clinical significance and biological function of UBR5 in colorectal cancer (CRC) are poorly understood. In this study, we compared the expression pattern of UBR5 between CRC and adjacent normal tissues and found that UBR5 expression was frequently elevated in CRC, possibly through chromosomal gains. Using three CRC patient cohorts, we found that patients with high UBR5 mRNA levels, UBR5 gene amplification, or high nuclear UBR5 protein levels had poor prognoses...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29258515/production-of-fluorescent-and-cytotoxic-k28-killer-toxin-variants-through-high-cell-density-fermentation-of-recombinant-pichia-pastoris
#3
Esther Giesselmann, Björn Becker, Manfred J Schmitt
BACKGROUND: Virus infected killer strains of the baker's yeast Saccharomyces cerevisiae secrete protein toxins such as K28, K1, K2 and Klus which are lethal to sensitive yeast strains of the same or related species. K28 is somewhat unique as it represents an α/β heterodimeric protein of the A/B toxin family which, after having bound to the surface of sensitive target cells, is taken up by receptor-mediated endocytosis and transported through the secretory pathway in a retrograde manner...
December 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29251729/virus-stamping-for-targeted-single-cell-infection-in-vitro-and-in-vivo
#4
Rajib Schubert, Stuart Trenholm, Kamill Balint, Georg Kosche, Cameron S Cowan, Manuel A Mohr, Martin Munz, David Martinez-Martin, Gotthold Fläschner, Richard Newton, Jacek Krol, Brigitte Gross Scherf, Keisuke Yonehara, Adrian Wertz, Aaron Ponti, Alexander Ghanem, Daniel Hillier, Karl-Klaus Conzelmann, Daniel J Müller, Botond Roska
Genetic engineering by viral infection of single cells is useful to study complex systems such as the brain. However, available methods for infecting single cells have drawbacks that limit their applications. Here we describe 'virus stamping', in which viruses are reversibly bound to a delivery vehicle-a functionalized glass pipette tip or magnetic nanoparticles in a pipette-that is brought into physical contact with the target cell on a surface or in tissue, using mechanical or magnetic forces. Different single cells in the same tissue can be infected with different viruses and an individual cell can be simultaneously infected with different viruses...
December 18, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29245973/microrna-433-inhibits-oral-squamous-cell-carcinoma-cells-by-targeting-fak
#5
Yong-Jian Wang, Zi-Feng Zhang, Shao-Hua Fan, Juan Zhuang, Qun Shan, Xin-Rui Han, Xin Wen, Meng-Qiu Li, Bin Hu, Chun-Hui Sun, Bin Qiao, Qian Tao, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
We investigated the involvement of microRNA-433 (miR-433) in the proliferation, migration, and invasiveness of oral squamous cell carcinoma (OSCC). Totally 108 OSCC tissues and adjacent normal tissues from patients with OSCC were collected. Also, transplanted tumor formation experiment in nude mice was conducted to verify the effect of miR-433 and FAK on subcutaneous transplanted tumor. The CD44+ stem cell from SCC-9 were collected and assigned into the blank, miR-433 mimics, mimics control, miR-433 inhibitors, inhibitors control, siFAK and miR-433 inhibitors + siFAK groups...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29230248/a-concise-review-on-the-use-of-mesenchymal-stem-cells-in-cell-sheet-based-tissue-engineering-with-special-emphasis-on-bone-tissue-regeneration
#6
REVIEW
A Cagdas Yorukoglu, A Esat Kiter, Semih Akkaya, N Lale Satiroglu-Tufan, A Cevik Tufan
The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29221121/tropomyosin-isoform-tpm2-1-regulates-collective-and-amoeboid-cell-migration-and-cell-aggregation-in-breast-epithelial-cells
#7
HyeRim Shin, Dayoung Kim, David M Helfman
Metastasis dissemination is the result of various processes including cell migration and cell aggregation. These processes involve alterations in the expression and organization of cytoskeletal and adhesion proteins in tumor cells. Alterations in actin filaments and their binding partners are known to be key players in metastasis. Downregulation of specific tropomyosin (Tpm) isoforms is a common characteristic of transformed cells. In this study, we examined the role of Tpm2.1 in non-transformed MCF10A breast epithelial cells in cell migration and cell aggregation, because this isoform is downregulated in primary and metastatic breast cancer as well as various breast cancer cell lines...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212227/shift-in-energy-metabolism-caused-by-glucocorticoids-enhances-the-effect-of-cytotoxic-anti-cancer-drugs-against-acute-lymphoblastic-leukemia-cells
#8
Shigeki Aoki, Michie Morita, Takuya Hirao, Masashi Yamaguchi, Reika Shiratori, Megumi Kikuya, Hiroji Chibana, Kousei Ito
Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy. Treatments include glucocorticoids (GCs) such as dexamethasone (Dex) and prednisolone, which may be of value when used alongside cytotoxic anti-cancer drugs. To predict therapeutic efficacy of GCs, their activity against ALL cells is usually examined prior to chemotherapy; however, few studies have examined their effects when used in combination with other drugs. The paradox is that cytotoxic anti-cancer drugs that are effective against proliferating cancer cells show synergistic effects when used with GCs that prevent cell proliferation...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29198709/cholesterol-inhibits-entotic-cell-in-cell-formation-and-actomyosin-contraction
#9
Banzhan Ruan, Bo Zhang, Ang Chen, Long Yuan, Jianqing Liang, Manna Wang, Zhengrong Zhang, Jie Fan, Xiaochen Yu, Xin Zhang, Zubiao Niu, You Zheng, Songzhi Gu, Xiaoqing Liu, Hongli Du, Jufang Wang, Xianwen Hu, Lihua Gao, Zhaolie Chen, Hongyan Huang, Xiaoning Wang, Qiang Sun
Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cell-in-cell structures by utilizing liposome as a vector. We found that Lipofectamine-2000, the reagent commonly used for routine transfection, could significantly reduce entotic cell-in-cell formation in a cell-specific manner, which is correlated with suppressed actomyosin contraction as indicated by reduced β-actin expression and myosin light chain phosphorylation...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29116180/the-ephb6-receptor-is-overexpressed-in-pediatric-t-cell-acute-lymphoblastic-leukemia-and-increases-its-sensitivity-to-doxorubicin-treatment
#10
Amr El Zawily, Emily McEwen, Behzad Toosi, Frederick S Vizeacoumar, Tanya Freywald, Franco J Vizeacoumar, Andrew Freywald
While impressive improvements have been achieved in T-ALL therapy, current treatment approaches fail in approximately 25% of patients and these patients have limited treatment options. Another significant group of patients is being overtreated, which causes long-lasting side effects. Identification of molecules controlling drug resistance in T-ALL is crucial for treatment optimisation in both scenarios. We report here the EphB6 receptor is frequently overexpressed in T-ALL. Remarkably, our observations indicate that EphB6 acts in T-ALL cells to enhance sensitivity to a DNA-damaging drug, doxorubicin, as interruption of EphB6 activity interferes with the efficiency of doxorubicin-induced eradication of T-ALL cells in cell culture and in xenograft animals...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29067774/recellularization-of-well-preserved-decellularized-kidney-scaffold-using-adipose-tissue-derived-stem-cells
#11
Aibing Xue, Guangzhu Niu, Yuan Chen, Kailin Li, Zhiying Xiao, Yun Luan, Chao Sun, Xiaoshuai Xie, Denglu Zhang, Xiaohang Du, Feng Kong, Yanxia Guo, Haiyang Zhang, Guanghui Cheng, Qian Xin, Yong Guan, Shengtian Zhao
To establish a recellularization kidney model by using adipose tissue-derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate (SDS). Immunofluorescence, immunohistochemisry and scanning electron microscope were conducted to examine the scaffold microstructure...
October 25, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29062241/interleukin-1-receptor-antagonist-inhibits-angiogenesis-via-blockage-il-1%C3%AE-pi3k-nf-%C3%AE%C2%BA%C3%AE-pathway-in-human-colon-cancer-cell
#12
Jiachi Ma, Xiaowen Sun, Tiankang Guo, He Su, Quan Chen, Zhenqiang Gong, Jianbo Qi, Xiaodan Zhao
PURPOSE: This article investigates the relationship between cancer cells and stromal cells in carcinoma cell living microenvironment and elucidates the mechanism that interleukin-1 receptor antagonist (IL-1RA) blocks metastatic potential in colon cancer. METHODS: Western blot and RT-PCR assay were used to determine the expression of hepatocyte growth factor (HGF) and IL-1α in colon carcinoma cells and stromal cells. Effect of IL-1RA and HGF on metastatic potential of colon cancer cells were examined by proliferation, invasion, and angiogenesis assays...
2017: Cancer Management and Research
https://www.readbyqxmd.com/read/29046462/distinct-roles-of-cellular-escrt-i-and-escrt-iii-proteins-in-efficient-entry-and-egress-of-budded-virions-of-autographa-californica-multiple-nucleopolyhedrovirus
#13
Qi Yue, Qianlong Yu, Qi Yang, Ye Xu, Ya Guo, Gary W Blissard, Zhaofei Li
The endosomal sorting complex required for transport (ESCRT) machinery is necessary for budding by many enveloped viruses. Recently, it was demonstrated that Vps4, the key regulator for recycling of the ESCRT-III complex, is required for efficient infection of the baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, ESCRT assembly, regulation and function are complex and little is known regarding details of participation of specific ESCRT complexes in AcMNPV infection. In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system and RNAi assays, we found that ESCRT-I and ESCRT-III complexes are required for efficient entry of AcMNPV into insect cells...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28989066/interleukin-35-expression-correlates-with-microvessel-density-in-pancreatic-ductal-adenocarcinoma-recruits-monocytes-and-promotes-growth-and-angiogenesis-of-xenograft-tumors-in-mice
#14
Chongbiao Huang, Zengxun Li, Na Li, Yang Li, Antao Chang, Tiansuo Zhao, Xiuchao Wang, Hongwei Wang, Song Gao, Shengyu Yang, Jihui Hao, He Ren
BACKGROUND & AIMS: Cells of the monocyte lineage contribute to tumor angiogenesis. Interleukin 35 (IL35) is a member of the IL12 family produced by regulatory, but not effector, T cells. IL35 is a dimer comprising the IL12 alpha and IL27 beta chains, encoded by IL12A and EBI3, respectively. Expression of IL35 is increased in pancreatic ductal adenocarcinomas (PDACs) compared with normal pancreatic tissues, and promotes metastasis. We investigated the role of IL-35 in monocyte-induced angiogenesis of PDAC in mice...
October 5, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28987793/silver-nanoparticles-induce-lysosomal-autophagic-defects-and-decreased-expression-of-transcription-factor-eb-in-a549-human-lung-adenocarcinoma-cells
#15
Takamitsu Miyayama, Kota Fujiki, Masato Matsuoka
Although silver nanoparticles (AgNPs) are widely used in consumer and medical products, the mechanism by which AgNPs cause pulmonary damage is unclear. AgNPs are incorporated into cells and processed via the autophagy pathway. We examined the effects of AgNP exposure on autophagic flux and expression of transcription factor EB (TFEB) in A549 lung adenocarcinoma cells. In cells exposed to citrate-coated 60-nm AgNPs, confocal laser microscopic examination showed a decrease in the LysoTracker fluorescence signal and an increase in that of Cyto-ID, indicating lysosomal pH alkalization and autophagosome formation, respectively...
October 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28983606/parp%C3%A2-1-may-be-involved-in-hydroquinone%C3%A2-induced-apoptosis-by-poly-adp%C3%A2-ribosylation-of-zo%C3%A2-2
#16
Jiaxian Liu, Qian Yuan, Xiaoxuan Ling, Qiang Tan, Hairong Liang, Jialong Chen, Lianzai Lin, Yongmei Xiao, Wen Chen, Linhua Liu, Huanwen Tang
Hydroquinone (HQ), a major reactive metabolite of benzene, contributes to benzene‑induced leukemia. The molecular mechanisms that underlie this activity remain to be elucidated. Poly ADP‑ribosylation (PARylation) is a type of reversible posttranslational modification that is performed by enzymes in the PAR polymerase (PARP) family and mediates different biological processes, including apoptosis. Zona occludens 2 (ZO‑2) is a tight junction scaffold protein, which is involved in cell proliferation and apoptosis...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28978069/cell-cycle-perturbation-induced-by-gemcitabine-in-human-tumor-cells-in-cell-culture-xenografts-and-bladder-cancer-patients-implications-for-clinical-trial-designs-combining-gemcitabine-with-a-chk1-inhibitor
#17
Ryan Montano, Nadeem Khan, Huagang Hou, John Seigne, Marc S Ernstoff, Lionel D Lewis, Alan Eastman
Gemcitabine irreversibly inhibits ribonucleotide reductase and induces S phase arrest but whether this occurs in tumors in mice or patients has not been established. Tumor cells in culture were incubated with gemcitabine for 6 h to approximate the administration schedule in a patient. Concentrations that induced persistent S phase arrest thereafter correlated with cell killing. Administration of gemcitabine to mice also demonstrated a persistent S phase arrest in their tumor. The minimum dose that induced almost complete S phase arrest after 24 h (40 mg/kg) was well below the maximum tolerated dose in mice...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28970591/consecutive-entosis-stages-in-human-substrate-dependent-cultured-cells
#18
Anastasiia S Garanina, Olga P Kisurina-Evgenieva, Maria V Erokhina, Elena A Smirnova, Valentina M Factor, Galina E Onishchenko
Entosis, or cell death by invading another cell, is typical for tumor epithelial cells. The formation of cell-in-cell structures is extensively studied in suspension cultures, but remains poorly understood in substrate-dependent cells. Here, we used electron, confocal and time-lapse microscopy in combination with pharmacological inhibition of intracellular components to study the kinetics of entosis using two human substrate-dependent tumor cultures, A431 and MCF7. In total, we identified and characterized five consecutive stages of entosis, which were common for both examined cell lines...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28967645/association-of-cd44-cd24-low-with-markers-of-aggressiveness-and-plasticity-of-cell-lines-and%C3%A2-tumors-of-patients-with-breast-cancer
#19
V F Chekhun, N Y Lukianova, S V Chekhun, N O Bezdieniezhnykh, T V Zadvorniy, T V Borikun, L Z Polishchuk, O М Klyusov
AIM: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. MATERIALS AND METHODS: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method...
September 2017: Experimental Oncology
https://www.readbyqxmd.com/read/28932655/ultrasmall-conjugated-polymer-nanoparticles-with-high-specificity-for-targeted-cancer-cell-imaging
#20
Guangxue Feng, Jie Liu, Rongrong Liu, Duo Mao, Nikodem Tomczak, Bin Liu
Fluorescent and biocompatible organic nanoparticles have attracted great interest in cancer detection and imaging, but the nonspecific cellular uptake has limited the detection specificity and sensitivity. Herein, the authors report the ultrasmall conjugated polymer nanoparticles (CPNs) with bright far-red/near-infrared emission for targeted cancer imaging with high specificity. The sizes of the ultrasmall CPNs are around 6 nm (CPN6), while large CPNs show sizes around 30 nm (CPN30). Moreover, CPN6 exhibits largely improved fluorescence quantum yield (η) of 41% than CPN30 (25%)...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
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