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https://www.readbyqxmd.com/read/29116180/the-ephb6-receptor-is-overexpressed-in-pediatric-t-cell-acute-lymphoblastic-leukemia-and-increases-its-sensitivity-to-doxorubicin-treatment
#1
Amr El Zawily, Emily McEwen, Behzad Toosi, Frederick S Vizeacoumar, Tanya Freywald, Franco J Vizeacoumar, Andrew Freywald
While impressive improvements have been achieved in T-ALL therapy, current treatment approaches fail in approximately 25% of patients and these patients have limited treatment options. Another significant group of patients is being overtreated, which causes long-lasting side effects. Identification of molecules controlling drug resistance in T-ALL is crucial for treatment optimisation in both scenarios. We report here the EphB6 receptor is frequently overexpressed in T-ALL. Remarkably, our observations indicate that EphB6 acts in T-ALL cells to enhance sensitivity to a DNA-damaging drug, doxorubicin, as interruption of EphB6 activity interferes with the efficiency of doxorubicin-induced eradication of T-ALL cells in cell culture and in xenograft animals...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29067774/recellularization-of-well-preserved-decellularized-kidney-scaffold-using-adipose-tissue-derived-stem-cells
#2
Aibing Xue, Guangzhu Niu, Yuan Chen, Kailin Li, Zhiying Xiao, Yun Luan, Chao Sun, Xiaoshuai Xie, Denglu Zhang, Xiaohang Du, Feng Kong, Yanxia Guo, Haiyang Zhang, Guanghui Cheng, Qian Xin, Yong Guan, Shengtian Zhao
To establish a recellularization kidney model by using adipose tissue-derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate (SDS). Immunofluorescence, immunohistochemisry and scanning electron microscope were conducted to examine the scaffold microstructure...
October 25, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29062241/interleukin-1-receptor-antagonist-inhibits-angiogenesis-via-blockage-il-1%C3%AE-pi3k-nf-%C3%AE%C2%BA%C3%AE-pathway-in-human-colon-cancer-cell
#3
Jiachi Ma, Xiaowen Sun, Tiankang Guo, He Su, Quan Chen, Zhenqiang Gong, Jianbo Qi, Xiaodan Zhao
PURPOSE: This article investigates the relationship between cancer cells and stromal cells in carcinoma cell living microenvironment and elucidates the mechanism that interleukin-1 receptor antagonist (IL-1RA) blocks metastatic potential in colon cancer. METHODS: Western blot and RT-PCR assay were used to determine the expression of hepatocyte growth factor (HGF) and IL-1α in colon carcinoma cells and stromal cells. Effect of IL-1RA and HGF on metastatic potential of colon cancer cells were examined by proliferation, invasion, and angiogenesis assays...
2017: Cancer Management and Research
https://www.readbyqxmd.com/read/29046462/distinct-roles-of-cellular-escrt-i-and-escrt-iii-proteins-in-efficient-entry-and-egress-of-budded-virions-of-autographa-californica-multiple-nucleopolyhedrovirus
#4
Qi Yue, Qianlong Yu, Qi Yang, Ye Xu, Ya Guo, Gary W Blissard, Zhaofei Li
The endosomal sorting complex required for transport (ESCRT) machinery is necessary for budding by many enveloped viruses. Recently, it was demonstrated that Vps4, the key regulator for recycling of the ESCRT-III complex, is required for efficient infection of the baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, ESCRT assembly, regulation and function are complex and little is known regarding details of participation of specific ESCRT complexes in AcMNPV infection. In this study, the core components of ESCRT-I (Tsg101 and Vps28) and ESCRT-III (Vps2B, Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral complementation system and RNAi assays, we found that ESCRT-I and ESCRT-III complexes are required for efficient entry of AcMNPV into insect cells...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28989066/interleukin-35-expression-correlates-with-microvessel-density-in-pancreatic-ductal-adenocarcinoma-recruits-monocytes-and-promotes-growth-and-angiogenesis-of-xenograft-tumors-in-mice
#5
Chongbiao Huang, Zengxun Li, Na Li, Yang Li, Antao Chang, Tiansuo Zhao, Xiuchao Wang, Hongwei Wang, Song Gao, Shengyu Yang, Jihui Hao, He Ren
BACKGROUND & AIMS: Cells of the monocyte lineage contribute to tumor angiogenesis. Interleukin 35 (IL35) is a member of the IL12 family produced by regulatory, but not effector, T cells. IL35 is a dimer comprising the IL12 alpha and IL27 beta chains, encoded by IL12A and EBI3, respectively. Expression of IL35 is increased in pancreatic ductal adenocarcinomas (PDACs) compared with normal pancreatic tissues, and promotes metastasis. We investigated the role of IL-35 in monocyte-induced angiogenesis of PDAC in mice...
October 5, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28987793/silver-nanoparticles-induce-lysosomal-autophagic-defects-and-decreased-expression-of-transcription-factor-eb-in-a549-human-lung-adenocarcinoma-cells
#6
Takamitsu Miyayama, Kota Fujiki, Masato Matsuoka
Although silver nanoparticles (AgNPs) are widely used in consumer and medical products, the mechanism by which AgNPs cause pulmonary damage is unclear. AgNPs are incorporated into cells and processed via the autophagy pathway. We examined the effects of AgNP exposure on autophagic flux and expression of transcription factor EB (TFEB) in A549 lung adenocarcinoma cells. In cells exposed to citrate-coated 60-nm AgNPs, confocal laser microscopic examination showed a decrease in the LysoTracker fluorescence signal and an increase in that of Cyto-ID, indicating lysosomal pH alkalization and autophagosome formation, respectively...
October 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28983606/parp%C3%A2-1-may-be-involved-in-hydroquinone%C3%A2-induced-apoptosis-by-poly-adp%C3%A2-ribosylation-of-zo%C3%A2-2
#7
Jiaxian Liu, Qian Yuan, Xiaoxuan Ling, Qiang Tan, Hairong Liang, Jialong Chen, Lianzai Lin, Yongmei Xiao, Wen Chen, Linhua Liu, Huanwen Tang
Hydroquinone (HQ), a major reactive metabolite of benzene, contributes to benzene‑induced leukemia. The molecular mechanisms that underlie this activity remain to be elucidated. Poly ADP‑ribosylation (PARylation) is a type of reversible posttranslational modification that is performed by enzymes in the PAR polymerase (PARP) family and mediates different biological processes, including apoptosis. Zona occludens 2 (ZO‑2) is a tight junction scaffold protein, which is involved in cell proliferation and apoptosis...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28978069/cell-cycle-perturbation-induced-by-gemcitabine-in-human-tumor-cells-in-cell-culture-xenografts-and-bladder-cancer-patients-implications-for-clinical-trial-designs-combining-gemcitabine-with-a-chk1-inhibitor
#8
Ryan Montano, Nadeem Khan, Huagang Hou, John Seigne, Marc S Ernstoff, Lionel D Lewis, Alan Eastman
Gemcitabine irreversibly inhibits ribonucleotide reductase and induces S phase arrest but whether this occurs in tumors in mice or patients has not been established. Tumor cells in culture were incubated with gemcitabine for 6 h to approximate the administration schedule in a patient. Concentrations that induced persistent S phase arrest thereafter correlated with cell killing. Administration of gemcitabine to mice also demonstrated a persistent S phase arrest in their tumor. The minimum dose that induced almost complete S phase arrest after 24 h (40 mg/kg) was well below the maximum tolerated dose in mice...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28970591/consecutive-entosis-stages-in-human-substrate-dependent-cultured-cells
#9
Anastasiia S Garanina, Olga P Kisurina-Evgenieva, Maria V Erokhina, Elena A Smirnova, Valentina M Factor, Galina E Onishchenko
Entosis, or cell death by invading another cell, is typical for tumor epithelial cells. The formation of cell-in-cell structures is extensively studied in suspension cultures, but remains poorly understood in substrate-dependent cells. Here, we used electron, confocal and time-lapse microscopy in combination with pharmacological inhibition of intracellular components to study the kinetics of entosis using two human substrate-dependent tumor cultures, A431 and MCF7. In total, we identified and characterized five consecutive stages of entosis, which were common for both examined cell lines...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28967645/association-of-cd44-cd24-low-with-markers-of-aggressiveness-and-plasticity-of-cell-lines-and%C3%A2-tumors-of-patients-with-breast-cancer
#10
V F Chekhun, N Y Lukianova, S V Chekhun, N O Bezdieniezhnykh, T V Zadvorniy, T V Borikun, L Z Polishchuk, O М Klyusov
AIM: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. MATERIALS AND METHODS: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method...
September 2017: Experimental Oncology
https://www.readbyqxmd.com/read/28932655/ultrasmall-conjugated-polymer-nanoparticles-with-high-specificity-for-targeted-cancer-cell-imaging
#11
Guangxue Feng, Jie Liu, Rongrong Liu, Duo Mao, Nikodem Tomczak, Bin Liu
Fluorescent and biocompatible organic nanoparticles have attracted great interest in cancer detection and imaging, but the nonspecific cellular uptake has limited the detection specificity and sensitivity. Herein, the authors report the ultrasmall conjugated polymer nanoparticles (CPNs) with bright far-red/near-infrared emission for targeted cancer imaging with high specificity. The sizes of the ultrasmall CPNs are around 6 nm (CPN6), while large CPNs show sizes around 30 nm (CPN30). Moreover, CPN6 exhibits largely improved fluorescence quantum yield (η) of 41% than CPN30 (25%)...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28918743/eb-family-proteins-functions-and-microtubule-interaction-mechanisms
#12
REVIEW
V V Mustyatsa, A V Boyakhchyan, F I Ataullakhanov, N B Gudimchuk
Microtubules are polymers of tubulin protein, one of the key components of cytoskeleton. They are polar filaments whose plus-ends usually oriented toward the cell periphery are more dynamic than their minus-ends, which face the center of the cell. In cells, microtubules are organized into a network that is being constantly rebuilt and renovated due to stochastic switching of its individual filaments from growth to shrinkage and back. Because of these dynamics and their mechanical properties, microtubules take part in various essential processes, from intracellular transport to search and capture of chromosomes during mitosis...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28910941/-effects-of-src-on-cervical-cancer-cells-proliferation-and-apoptosis-through-erk-signal-transduction-pathway
#13
Z C Song, L Ding, Z Y Ren, X S Sun, Q Yang, L Wang, M J Feng, C L Liu, J T Wang
Objective: To explore the effect of Src on cervical cancer cells through ERK signal transduction pathway. Methods: Experimental study was carried out in vitro. Cervical cancer cell lines Hela (HPV-positive) and C33A (HPV-negative) were treated with Src kinase inhibitor PP2. Then, the cell cycle and apoptosis of each group were evaluated by using flow cytometry (FCM). Western blotting and Real-time PCR were used to detect the levels of the expression of ERK 1/2, c-Fos and c-Jun mRNA and protein respectively...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28880014/chimeric-antigen-receptor-car-transduced-natural-killer-cells-in-tumor-immunotherapy
#14
REVIEW
Yuan Hu, Zhi-Gang Tian, Cai Zhang
Natural killer (NK) cells are potential effector cells in cell-based cancer immunotherapy, particularly in the control of hematological malignancies. The chimeric antigen receptor (CAR) is an artificially modified fusion protein that consists of an extracellular antigen recognition domain fused to an intracellular signaling domain. T cells genetically modified with a CAR have demonstrated remarkable success in the treatment of hematological cancers. Compared to T cells, CAR-transduced NK cells (CAR-NK) exhibit several advantages, such as safety in clinical use, the mechanisms by which they recognize cancer cells, and their abundance in clinical samples...
September 7, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28864659/a-novel-role-for-ceramide-synthase-6-in-mouse-and-human-alcoholic-steatosis
#15
Bianca Williams, Jason Correnti, Amanke Oranu, Annie Lin, Victoria Scott, Maxine Annoh, James Beck, Emma Furth, Victoria Mitchell, Can E Senkal, Lina Obeid, Rotonya M Carr
Perilipin 2 (PLIN2) is a lipid-droplet protein that is up-regulated in alcoholic steatosis and associated with hepatic accumulation of ceramides, bioactive lipids implicated in alcoholic liver disease pathogenesis. The specific role of ceramide synthetic enzymes in the regulation of PLIN2 and promotion of hepatocellular lipid accumulation is not well understood. We examined the effects of pharmacologic ceramide synthesis inhibition on hepatic PLIN2 expression, steatosis, and glucose and lipid homeostasis in mice with alcoholic steatosis and in ethanol-incubated human hepatoma VL17A cells...
September 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28774893/mrtf-transcription-and-ezrin-dependent-plasma-membrane-blebbing-are-required-for-entotic-invasion
#16
Laura Soto Hinojosa, Manuel Holst, Christian Baarlink, Robert Grosse
Entosis is a nonapoptotic form of cell death initiated by actomyosin-dependent homotypic cell-in-cell invasion that can be observed in malignant exudates during tumor progression. We previously demonstrated formin-mediated actin dynamics at the rear of the invading cell as well as nonapoptotic plasma membrane (PM) blebbing in this cellular motile process. Although the contractile actin cortex involved in bleb-driven motility is well characterized, a role for transcriptional regulation in this process has not been studied...
October 2, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28764725/distinct-effects-of-egfr-inhibitors-on-epithelial-and-mesenchymal-like-esophageal-squamous-cell-carcinoma-cells
#17
Masahiro Yoshioka, Shinya Ohashi, Tomomi Ida, Yukie Nakai, Osamu Kikuchi, Yusuke Amanuma, Junichi Matsubara, Atsushi Yamada, Shin'ichi Miyamoto, Mitsuteru Natsuizaka, Hiroshi Nakagawa, Tsutomu Chiba, Hiroshi Seno, Manabu Muto
BACKGROUND: Epidermal growth factor receptor (EGFR) plays a pivotal role in the pathophysiology of esophageal squamous cell carcinoma (ESCC). However, the clinical effects of EGFR inhibitors on ESCC are controversial. This study sought to identify the factors determining the therapeutic efficacy of EGFR inhibitors in ESCC cells. METHODS: Immortalized-human esophageal epithelial cells (EPC2-hTERT), transformed-human esophageal epithelial cells (T-Epi and T-Mes), and ESCC cells (TE-1, TE-5, TE-8, TE-11, TE-11R, and HCE4) were treated with the EGFR inhibitors erlotinib or cetuximab...
August 1, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28760079/preparation-of-cell-aggregates-incorporating-gelatin-hydrogel-microspheres-containing-bone-morphogenic-protein-2-with-different-degradabilities
#18
Shuhei Tajima, Yasuhiko Tabata
The objective of this study is to evaluate the survival and functions of cells in cell aggregates incorporating gelatin hydrogel microspheres (GM) containing bone morphogenic protein-2 (BMP2). Gelatin was dehydrothermally crosslinked in a water-in-oil emulsion state at 140 °C for various time periods to prepare GM with different degradabilities. BMP2 was dropped onto the GM freeze dried, followed by leaving at 25 °C to obtain GM containing BMP2 (GM-BMP2). MC3T3-E1 cells were cultured with GM-BMP2 and GM in round U-bottom wells of 96-multiwell microplates which had been coated with poly (vinyl alcohol) (PVA), to allow to form cell aggregates containing GM-BMP2 and GM, respectively...
July 31, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28747257/cells-with-treg-specific-foxp3-demethylation-but-low-cd25-are-prevalent-in-autoimmunity
#19
Ricardo C Ferreira, Henry Z Simons, Whitney S Thompson, Daniel B Rainbow, Xin Yang, Antony J Cutler, Joao Oliveira, Xaquin Castro Dopico, Deborah J Smyth, Natalia Savinykh, Meghavi Mashar, Tim J Vyse, David B Dunger, Helen Baxendale, Anita Chandra, Chris Wallace, John A Todd, Linda S Wicker, Marcin L Pekalski
Identification of alterations in the cellular composition of the human immune system is key to understanding the autoimmune process. Recently, a subset of FOXP3(+) cells with low CD25 expression was found to be increased in peripheral blood from systemic lupus erythematosus (SLE) patients, although its functional significance remains controversial. Here we find in comparisons with healthy donors that the frequency of FOXP3(+) cells within CD127(low)CD25(low) CD4(+) T cells (here defined as CD25(low)FOXP3(+) T cells) is increased in patients affected by autoimmune disease of varying severity, from combined immunodeficiency with active autoimmunity, SLE to type 1 diabetes...
November 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28732351/tropomyosin-isoform-tpm2-1-regulates-collective-and-amoeboid-cell-migration-and-cell-aggregation-in-breast-epithelial-cells
#20
HyeRim Shin, Dayoung Kim, David M Helfman
Metastasis dissemination is the result of various processes including cell migration and cell aggregation. These processes involve alterations in the expression and organization of cytoskeletal and adhesion proteins in tumor cells. Alterations in actin filaments and their binding partners are known to be key players in metastasis. Downregulation of specific tropomyosin (Tpm) isoforms is a common characteristic of transformed cells. In this study, we examined the role of Tpm2.1 in non-transformed MCF10A breast epithelial cells in cell migration and cell aggregation, because this isoform is downregulated in primary and metastatic breast cancer as well as various breast cancer cell lines...
July 12, 2017: Oncotarget
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