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https://www.readbyqxmd.com/read/28330331/identification-and-quantification-of-novel-rna-isoforms-in-horn-cancer-of-bos-indicus-by-comprehensive-rna-seq
#1
Subhash J Jakhesara, Prakash G Koringa, Neelam M Nathani, Chaitanya G Joshi
Horn cancer (HC) is a squamous cell carcinoma of horn, commonly observed in Bos indicus of the Asian countries. To elucidate the complexity of alternative splicing present in the HC, high-throughput sequencing and analysis of HC and matching horn normal (HN) tissue were carried out. A total of 535,067 and 849,077 reads were analysed after stringent quality filtering for HN and HC, respectively. Cufflinks pipeline for transcriptome analysis revealed 4786 novel splice isoforms comprising 2432 exclusively in HC, 2055 exclusively in HN and 298 in both the conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330310/diversity-and-bioactive-potentials-of-culturable-heterotrophic-bacteria-from-the-surficial-sediments-of-the-arabian-sea
#2
Abdulaziz Anas, Charulatha Nilayangod, C Jasmin, Saradavey Vinothkumar, P S Parameswaran, Shanta Nair
Marine sediments accommodate plethora of diverse microorganisms with varying ecological functions. In the present study, we isolated bacteria from surficial sediments of south east Arabian Sea (AS) and evaluated their bioactive potentials. A total of 131 isolates belonging to the phylum: γ-Proteobacteria (63%), Bacillales (34%) and Micrococcaceae (3%) were isolated. Among these, about 40% of the isolates showed the presence of secondary metabolite biosynthetic genes such as PKS or NRPS or both. Organic extracts of nearly 50% of these organisms were cytotoxic to human breast cancer MCF-7 cells and were bactericidal to human pathogens, Escherichia coli and Pseudomonas sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330304/elicitation-of-gymnemic-acid-production-in-cell-suspension-cultures-of-gymnema-sylvestre-r-br-through-endophytic-fungi
#3
Vasudeva Reddy Netala, Venkata Subbaiah Kotakadi, Susmila Aparna Gaddam, Sukhendu Bikash Ghosh, Vijaya Tartte
The enhancement of plant secondary metabolite production in cell suspension cultures through biotic or abiotic elicitation has become a potential biotechnological approach for commercialization or large-scale production of bioactive compounds. Gymnema sylvestre R.Br. is an important medicinal plant, rich in a group of oleanane triterpenoid saponins called gymnemic acid, well known for its anti-diabetic activity. Two endophytic fungal strains were isolated from the leaves of G. sylvestre and identified as Polyancora globosa and Xylaria sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330300/carbon-quantum-dots-shuttle-electrons-to-the-anode-of-a-microbial-fuel-cell
#4
A S Vishwanathan, Kartik S Aiyer, L A A Chunduri, K Venkataramaniah, S Siva Sankara Sai, Govind Rao
Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and carboxyl functional groups on their surface. The presence of these functional groups on a carbon matrix conferred on the C-dots the ability to conduct and transfer electrons. Formation of silver nanoparticles from silver nitrate upon addition of C-dots confirmed their reducing ability...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330295/biomass-derived-molecules-modulate-the-behavior-of-streptomyces-coelicolor-for-antibiotic-production
#5
Shashi Kant Bhatia, Bo-Rahm Lee, Ganesan Sathiyanarayanan, Hun Seok Song, Junyoung Kim, Jong-Min Jeon, Jeong-Jun Yoon, Jungoh Ahn, Kyungmoon Park, Yung-Hun Yang
Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC 50 value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330290/successful-cloning-of-an-adult-breeding-boar-from-the-novel-chinese-guike-no-1-swine-specialized-strain
#6
Jun-Yu Nie, Xiang-Xing Zhu, Bing-Kun Xie, Su-Qun Nong, Qing-Yan Ma, Hui-Yan Xu, Xiao-Gan Yang, Yang-Qing Lu, Ke-Huan Lu, Yu-Ying Liao, Sheng-Sheng Lu
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed is a novel swine specialized strain incorporated with the pedigree background of Duroc and Chinese Luchuan pig breeds, thus inherits an excellent production performance. The present study was conducted to establish somatic cloning procedures of adult breeding boars from the Chinese Guike No...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330289/progress-in-genome-sequencing-will-accelerate-molecular-breeding-in-cotton-gossypium-spp
#7
REVIEW
Rong Yan, Chengzhen Liang, Zhigang Meng, Waqas Malik, Tao Zhu, Xuefeng Zong, Sandui Guo, Rui Zhang
Cotton (Gossypium spp.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development. Thus, the Cotton Genome Consortium has made rapid and significant progress in whole genome sequencing studies in the last decade. Developments in cotton genome sequencing and assembly provide powerful tools for dissecting the genetic and molecular bases of agronomically important traits and establishing regulatory networks on these processes, which leads to molecular breeding...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330288/biofabrication-and-spectral-characterization-of-silver-nanoparticles-and-their-cytotoxic-studies-on-human-cd34-ve-stem-cells
#8
Venkata S Kotakadi, Susmila Aparna Gaddam, Sucharitha K Venkata, P V G K Sarma, D V R Sai Gopal
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330284/in-vitro-evaluation-of-anti-proliferative-anti-inflammatory-and-pro-apoptotic-activities-of-the-methanolic-extracts-of-andrographis-nallamalayana-ellis-on-a375-and-b16f10-melanoma-cell-lines
#9
Guroji Purushotham, Yerukala Padma, Yusuf Nabiha, R R Venkata Raju
Natural plant products have been widely used in controlling cancer with fewer or no side effects and the use of plant extracts as complementary to synthetic medicine is gaining increased popularity. Members of Andrographis plants possess important medicinal properties. In the present study, anti-cancerous properties of Andrographis nallamalayana (AN) were tested on A375 and B16F10 skin melanoma cancer cell lines. The leaf extracts of AN significantly reduced the cell viability and cell survival of skin cancer cell lines, achieved by MTT assay and clonogenic assays, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330282/in-vitro-cytotoxicity-and-antibacterial-activity-of-silver-coated-electrospun-polycaprolactone-gelatine-nanofibrous-scaffolds
#10
Mim Mim Lim, Naznin Sultana
The development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. In this study, polycaprolactone (PCL) and PCL/gelatine (Ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. Nanofibrous scaffolds were coated with silver (Ag) in different concentrations of silver nitrate (AgNO3) aqueous solution (1.25, 2.5, 5, and 10 %) by using dipping method, drying and followed by ultraviolet (UV) photoreduction...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#11
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330271/assessment-of-protein-silver-nanoparticles-toxicity-against-pathogenic-alternaria-solani
#12
Sobhy I I Abdel-Hafez, Nivien A Nafady, Ismail R Abdel-Rahim, Abeer M Shaltout, José-Antonio Daròs, Mohamed A Mohamed
Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract of endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were characterized by means of spectroscopic and microscopic techniques. The surface plasmon resonance found at 430 nm confirmed the formation of stable AgNPs for several weeks at room temperature. Also, the results revealed the formation of spherical and monodispersed AgNPs with an average size of 14...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330270/expression-and-purification-of-a-gene-encoding-a-9-7%C3%A2-kda-pe-protein-of-mycobacterium-avium-subsp-paratuberculosis
#13
S Chandra Sekar, P P Goswami, R Deb
Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. The recombinant plasmid designated as pQ PE was transformed into E. coli M15 cells and induced with IPTG revealed the high level expression of 11.9 kDa His-fusion protein as estimated by migration in 15 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330257/generation-of-an-inducible-system-to-express-polo-like-kinase-cdc5-as-tap-fusion-protein-during-meiosis-in-saccharomyces-cerevisiae
#14
Rajni Vaid, Kamal Dev, Michael Lichten, Anuradha Sourirajan
Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-like kinases (PLK) are serine/threonine protein kinases that are the crucial regulators of cell cycle. Cdc5, the solitary PLK in budding yeast Saccharomyces cerevisiae, has diverse array of targets in cell cycle. The present study was undertaken to construct an estrogen-inducible system for expression of Cdc5-TAP to isolate the substrates of Cdc5 during meiosis, particularly, pachytene stage of meiosis I...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330253/parameters-influencing-agrobacterium-mediated-transformation-system-in-safflower-genotypes-aks-207-and-pkv-pink
#15
Dipti Raghunath Dhumale, Prashant Raghunath Shingote, Mahendra Shankarrao Dudhare, Pravin Vishwanath Jadhav, Prashant Bhaskar Kale
Shoot regeneration in safflower (Carthamus tinctorius 'AKS 207' and 'PKV Pink') genetically transformed using Agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. Transformation frequency was promising with 8-10-day-old cotyledonary leaf explants. Therefore, explants of that age cultured on Agrobacterium minimal medium (AB) containing 100 µM acetosyringone were infected with Agrobacterium (cell titre 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330248/photo-inactivation-of-escherichia-coli-and-enterococcus-hirae-using-methylene-blue-and-sodium-anthraquinone-2-sulphonate-effect-of-process-parameters
#16
Madhavi Singh, Kannan Pakshirajan, Vishal Trivedi
In this study, effect of different parameters, viz. concentration of photosensitizer (PS), pH of the bacterial cell suspension and initial cell count, on photo-inactivation of Escherichia coli and Enterococcus hirae bacteria using methylene blue (MB) and sodium anthraquinone-2-sulphonate (SAQS) was investigated employing the statistically valid full factorial design of experiments. The inactivation efficiency of E. hirae using MB ranges between 10.81 and 48.55 %, whereas in the case of E. coli it ranges between 10...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330247/production-of-glucosinolates-phenolic-compounds-and-associated-gene-expression-profiles-of-hairy-root-cultures-in-turnip-brassica-rapa-ssp-rapa
#17
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR. GSLs levels (glucoallysin, glucobrassicanapin, gluconasturtiin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and 4-hydroxyglucobrassicin) and their gene expression levels (BrMYB28, BrMYB29, BrMYB34, BrMYB51, BrMYB122, CYP79 and CYP83) significantly increased in hairy roots compared with that in non-transformed roots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330230/isolation-and-biological-evaluation-of-n-4-aminocyclooctyl-3-5-dinitrobenzamide-a-new-semisynthetic-derivative-from-the-mangrove-associated-actinomycete-pseudonocardia-endophytica-vuk-10
#18
Usha Kiranmayi Mangamuri, Muvva Vijayalakshmi, Sudhakar Poda, Bramanandam Manavathi, Bhujangarao Chitturi, Venkateswarlu Yenamandra
The present study was aimed to isolate novel bioactive compounds from actinomycetes species isolated from mangrove habitats. With this connection, Pseudonocardia endophytica (VUK-10) was isolated using dilution plate technique and was examined for its secondary metabolite profiling. After successive purification and spectroscopic characterization viz., FTIR, mass, NMR, DEPT, HMQC, HMBC, and COSY spectroscopy, two compounds were identified including a semi synthetic derivative N-(4-aminocyclooctyl)-3, 5-dinitrobenzamide (1), obtained from the precursor of novel natural product cyclooctane-1,4-diamine (3), along with a known compound 3-((1H-indol-6-yl) methyl) hexahydropyrrolo [1, 2-a] pyrazine-1, 4-dione (2)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330214/production-kinetics-of-polyhydroxyalkanoates-by-using-pseudomonas-aeruginosa-gamma-ray-mutant-strain-ebn-8-cultured-on-soybean-oil
#19
Sharjeel Abid, Zulfiqar Ali Raza, Tanveer Hussain
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330200/use-of-ca-alginate-immobilized-pseudomonas-aeruginosa-for-repeated-batch-and-continuous-degradation-of-endosulfan
#20
Vijayalakshmi Pradeep, Usha Malavalli Subbaiah
The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was carried out with an objective of reducing the toxicity of Endosulfan by degrading it to less toxic metabolites. The long-term stability of Endosulfan degradation was studied during its repeated batch degradation, carried out over a period of 35 days...
December 2016: 3 Biotech
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