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preadipocyte AND adipogenesis

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https://www.readbyqxmd.com/read/27906176/epigenetic-programming-of-dnmt3a-mediated-by-ap2%C3%AE-is-required-for-granting-preadipocyte-the-ability-to-differentiate
#1
Wei Guo, Jiangnan Chen, Ying Yang, Jianbei Zhu, Jiarui Wu
Adipogenesis has an important role in regulating energy homeostasis in mammals. 3T3-L1 preadipocytes have been widely used as an in vitro model for analyzing the molecular mechanism of adipogenesis. Previous reports indicated that the stage of contact inhibition (CI), through which the proliferating cells exit from the cell cycle, was required for granting preadipocyte the ability to differentiate. While this kind of the granting mechanism remains elusive. In the present study, we showed that DNA (cytosine-5) methyltransferase 3a (Dnmt3a) was upregulated at both the mRNA and protein level during the CI stage, and resulted in increasing promoter methylation of adipogenic genes...
December 1, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27888346/methionine-and-cysteine-deficiencies-altered-proliferation-rate-and-time-course-differentiation-of-porcine-preadipose-cells
#2
Rosa Castellano, Marie-Hélène Perruchot, Sophie Tesseraud, Sonia Métayer-Coustard, Elizabeth Baeza, Yves Mercier, Florence Gondret
Methionine (Met) is an essential sulfur amino acid (AA) limiting growth and is the precursor of cysteine (Cys), the rate-limiting factor in the synthesis of glutathione, and the main intracellular non-enzymatic antioxidant. This study aimed at determining the effects of limited supplies in Met and(or) Cys in early aspects of adipose tissue development and oxidative stress in differentiated adipocytes. Incremental reductions in Met (70, 40, and 0 µM) were compared with Met 100 µM (control dose) in porcine preadipocytes cultured in media without or with Cys (250 µM)...
November 25, 2016: Amino Acids
https://www.readbyqxmd.com/read/27884413/peroxisome-proliferator-activated-receptor-%C3%AE-down-regulation-mediates-the-inhibitory-effect-of-d-%C3%AE-tocotrienol-on-the-differentiation-of-murine-3t3-f442a-preadipocytes
#3
Sheida Torabi, Hoda Yeganehjoo, Chwan-Li Shen, Huanbiao Mo
Tocotrienols accelerate the degradation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase that catalyzes the biosynthesis of mevalonate; the latter is essential for preadipocyte differentiation. Tocotrienols also down-regulate peroxisome proliferator-activated receptor γ (PPARγ), a key regulator of adipocyte differentiation. We hypothesized that mevalonate deprivation and PPARγ down-regulation mediate d-δ-tocotrienol-induced inhibition of adipocyte differentiation. The objectives of this study were to determine the effect of d-δ-tocotrienol on 3T3-F442A preadipocyte differentiation and the involvement of PPARγ and mevalonate...
November 3, 2016: Nutrition Research
https://www.readbyqxmd.com/read/27879248/high-fat-diet-induced-obesity-regulates-mmp3-to-modulate-depot-and-sex-dependent-adipose-expansion-in-c57bl-6j-mice
#4
Yuanyuan Wu, Mi-Jeong Lee, Yasuo Ido, Susan K Fried
Increased adipocyte size is hypothesized to signal the recruitment of adipose progenitor cells (APCs) to expand tissue storage capacity. To investigate depot- and sex-differences in adipose growth, male and female C57BL/6J mice (10-weeks-old) were challenged with high fat (HF) or low fat (LF) diets (D) for 14 weeks. The HFD increased gonadal (GON) depot weight by adipocyte hypertrophy and hyperplasia in females, but hypertrophy alone in males. In both sexes, inguinal (ING) adipocytes were smaller than GON and depot expansion was due to hypertrophy...
November 22, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27869309/roles-of-notch-signaling-in-adipocyte-progenitor-cells-and-mature-adipocytes
#5
REVIEW
Tizhong Shan, Jiaqi Liu, Weiche Wu, Ziye Xu, Yizhen Wang
Adipose tissues, composed with mature adipocytes and preadipocytic stromal/stem cells, play crucial roles in whole body energy metabolism and regenerative medicine. Mature adipocytes are derived and differentiated from mesenchymal stem cells (MSCs) or preadipocytes. This differentiation process, also called adipogenesis, is regulated by several signaling pathways and transcription factors. Notch1 signaling is a highly conserved pathway that is indispensable for stem cell hemostasis and tissue development. In adipocyte progenitor cells, Notch1 signaling regulates the adipogenesis process including proliferation and differentiation of the adipocyte progenitor cells in vitro...
November 21, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27867269/platyphylloside-isolated-from-betula-platyphylla-inhibit-adipocyte-differentiation-and-induce-lipolysis-via-regulating-adipokines-including-ppar%C3%AE-in-3t3-l1-cells
#6
Mina Lee, Sang Hyun Sung
BACKGROUND: Obesity causes or aggravates many health problems, both independently and in association with several pathological disorders, including Type II diabetes, hypertension, atherosclerosis, and cancer. Therefore, we screened small compounds isolated from natural products for the development of anti-obesity drugs. OBJECTIVE: The purpose of this study was to investigate the anti-adipogenic activities of platyphylloside, diarylheptanoid isolated from Betula platyphylla, which was selected based on the screening using 3T3-L1 cells...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27864366/siah2-mediates-early-events-in-commitment-to-an-adipogenic-pathway
#7
Gail Kilroy, David H Burk, Z Elizabeth Floyd
Adipose tissue expansion occurs by increasing the size of existing adipocytes or by increasing the number of adipocytes via adipogenesis. Adipose tissue dysfunction in obesity is associated with adipocyte hypertrophy and impaired adipogenesis. We recently demonstrated that deletion of the ubiquitin ligase Siah2 is associated with enlarged adipocytes in lean or obese mice. In this study, we find that adipogenesis is impaired in 3T3-L1 preadipocytes stably transfected with Siah2 shRNA and that overexpression of Siah2 in non-precursor fibroblasts promotes adipogenesis...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27856250/ectopical-expression-of-fabp4-gene-can-induce-bovine-muscle-derived-stem-cells-adipogenesis
#8
Le Zhang, Yanfang Zhao, Yue Ning, Hongbao Wang, Linsen Zan
Fatty acid binding protein 4 (FABP4) plays a key role in Fatty acid catabolism in mammals. Findings from our previous studies have indicated that FABP4 neither affect the differentiation of bovine preadipocytes nor does it change the expression of upstream genes. To investigate whether ectopically expressed FABP4 can induces Muscle-Derived Stem Cells (MDSCs) lipid synthesis and understand the regulatory mechanism behind it. In this study, adenoviruses infection is achieved to ectopically expressed FABP4 in bovine MDSCs, RNA-seq analyses at the very early stages of induction were performed to reveal gene expression level changes during MDSCs transdifferentiation...
November 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27830366/maturation-of-adipocytes-is-suppressed-by-fluid-shear-stress
#9
Jongyun Choi, Sei Young Lee, Yeong-Min Yoo, Chi Hyun Kim
Preadipocytes are mechano-responsive cells and their differentiation to adipocytes may be regulated by various types of physical stimulation. Understanding the mechanism of differentiation, which increases the number of adipocytes and lipid accumulation is important in the study of obesity-related diseases. In this study, we investigated the effects of physical stimulation at different stages of adipogenic differentiation using physiological levels of fluid shear stress. Preadipocytes were treated with dexamethasone, 3-isobutyl-1-methylxanthine and insulin for 3 days (induction period) and incubated for additional 6 days for maturation...
November 9, 2016: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27814669/laminin-alginate-beads-as-preadipocyte-carriers-to-enhance-adipogenesis-in-vitro-and-in-vivo
#10
Yu-Sheng Hsueh, Yo-Shen Chen, Hao-Chih Tai, Ondrej Mestak, Sung-Chuan Chao, Yen-Yu Chen, Ying Shih, Jung-Feng Lin, Ming-Jium Shieh, Feng-Huei Lin
The use of autologous fat grafting in breast reconstruction still requires optimization. Fat survival and calcification are the main issues that affect the outcomes of the procedure. In this study, a cell-based therapy utilizing laminin-alginate beads (LABs) as carriers was proposed to promote cell survival and adipogenesis by providing short-term physical support and facilitate nutrient diffusion of the implants. Laminin-modified alginate beads were fabricated by immobilizing laminin onto ring-opened alginate, used to encapsulate 3T3-L1 preadipocytes, and evaluated in vitro and in vivo...
November 4, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27806294/seipin-regulates-lipid-droplet-expansion-and-adipocyte-development-by-modulating-the-activity-of-glycerol-3-phosphate-acyltransferase
#11
Martin Pagac, Daniel E Cooper, Yanfei Qi, Ivan E Lukmantara, Hoi Yin Mak, Zengying Wu, Yuan Tian, Zhonghua Liu, Mona Lei, Ximing Du, Charles Ferguson, Damian Kotevski, Pawel Sadowski, Weiqin Chen, Salome Boroda, Thurl E Harris, George Liu, Robert G Parton, Xun Huang, Rosalind A Coleman, Hongyuan Yang
Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) is caused by loss-of-function mutations in SEIPIN, a protein implicated in both adipogenesis and lipid droplet expansion but whose molecular function remains obscure. Here, we identify physical and functional interactions between SEIPIN and microsomal isoforms of glycerol-3-phosphate acyltransferase (GPAT) in multiple organisms. Compared to controls, GPAT activity was elevated in SEIPIN-deficient cells and tissues and GPAT kinetic values were altered. Increased GPAT activity appears to underpin the block in adipogenesis and abnormal lipid droplet morphology associated with SEIPIN loss...
November 1, 2016: Cell Reports
https://www.readbyqxmd.com/read/27803314/promotion-of-adipogenesis-by-neuropeptide-y-during-the-later-stages-of-chicken-preadipocyte-differentiation
#12
Steven L Shipp, Mark A Cline, Elizabeth R Gilbert
Neuropeptide Y (NPY) promotes adipogenesis in both birds and mammals, although mechanisms in avians remain unclear. The objective of this study was thus to evaluate effects of NPY on chick preadipocyte proliferation and differentiation. Preadipocytes were treated with 0, 1, 10, or 100 nmol/L NPY and gene expression and cellular proliferation were evaluated at 12, 24, and 48 h. At 12 h posttreatment, mRNA abundance of topoisomerase II alpha (TOP2A), and thioredoxin-dependent peroxidase 2 was upregulated and NPY was downregulated in response to NPY (0 vs...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27780975/the-role-and-possible-mechanism-of-lncrna-u90926-in-modulating-3t3-l1-preadipocyte-differentiation
#13
J Chen, Y Liu, S Lu, L Yin, C Zong, S Cui, D Qin, Y Yang, Q Guan, X Li, X Wang
BACKGROUND: Obesity is a risk factor for metabolic diseases, while preadipocyte differentiation or adipogenesis is closely related to obesity occurrence. Long noncoding RNAs (lncRNAs) are a unique class of transcripts in regulation of a variety of biological processes. Using cDNA microarray, we found lncRNA U90926 is negatively correlated with 3T3-L1 preadipocyte differentiation. OBJECTIVE: The aim of this study was to explore the role of lncRNA U90926 (lnc-U90926) in adipogenesis and the underlying mechanisms...
October 26, 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27780754/ppar%C3%AE-agonists-induce-adipocyte-differentiation-by-modulating-the-expression-of-lipin-1-which-acts-as-a-ppar%C3%AE-phosphatase
#14
Jina Kim, Yu-Jin Lee, Jung Min Kim, So Young Lee, Myung-Ae Bae, Jin Hee Ahn, Dong Cho Han, Byoung-Mog Kwon
PPARγ agonists induced obesity in animal models as a side effect. Microarray experiments reveal that PPARγ agonist upregulates the expression of lipin-1 and this upregulation is correlated with the activity of the agonists. Lipin-1 induced by PPARγ agonists decreased the levels of PPARγ and ERK1/2 phosphorylation through direct interaction with these proteins in 3T3-L1 cells. In PPARγ agonist-treated 3T3-L1 preadipocytes, the knockdown of lipin-1 expression by small interfering RNA inhibited the adipogenesis that was induced by PPARγ agonists...
October 22, 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27777595/soshiho-tang-aqueous-extract-exerts-antiobesity-effects-in-high-fat-diet-fed-mice-and-inhibits-adipogenesis-in-3t3-l1-adipocytes
#15
Sae-Rom Yoo, Mee-Young Lee, Byoung-Kab Kang, Hyeun-Kyoo Shin, Soo-Jin Jeong
Soshiho-tang (SST; sho-saiko-to in Japanese; xiaochaihu-tang in Chinese) has generally been used to improve liver fibrosis- and cirrhosis-related symptoms in traditional Korean medicine. Although many studies have investigated the pharmacological properties of SST, its antiobesity effect has not been elucidated. Thus, our present study was carried out to evaluate the antiobesity effect of SST using a high fat diet- (HFD) induced obese mouse model and 3T3-L1 adipose cells. C57BL/6J mice were randomly divided into four groups (n = 6/group), normal diet (ND), HFD-fed group, and HFD- and SST-fed groups (S200: 200 mg/kg of SST; S600: 600 mg/kg of SST) and given HFD with or without SST extract for 8 weeks...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27777311/glucocorticoid-receptor-accelerates-but-is-dispensable-for-adipogenesis
#16
Young-Kwon Park, Kai Ge
Dexamethasone (DEX), a synthetic ligand for glucocorticoid receptor (GR), is routinely used to stimulate adipogenesis in culture. GR-depleted preadipocytes show adipogenesis defects one week after induction of differentiation. However, it has remained unclear whether GR is required for adipogenesis in vivo By deleting GR in precursors of brown adipocytes, we found unexpectedly that GR is dispensable for brown adipose tissue development in mice. In culture, GR-deficient primary or immortalized white and brown preadipocytes showed severely delayed adipogenesis one week after induction of differentiation...
October 24, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27777310/distinct-roles-of-transcription-factors-klf4-krox20-and-ppar%C3%AE-in-adipogenesis
#17
Young-Kwon Park, Limin Wang, Anne Giampietro, Binbin Lai, Ji-Eun Lee, Kai Ge
Much of our knowledge on adipogenesis comes from cell culture models of preadipocyte differentiation. Adipogenesis is induced by treating confluent preadipocytes with the adipogenic cocktail, which activates transcription factors (TFs) glucocorticoid receptor (GR) and CREB within minutes and increases expression of TFs C/EBPβ, C/EBPδ, KLF4 and Krox20 within hours. All of these TFs have been shown to be capable of promoting adipogenesis in culture when they are overexpressed. However, it has remained unclear whether endogenous KLF4 and Krox20 are required for adipogenesis in culture and in vivo Using conditional knockout mice and derived white and brown preadipocytes, we show that endogenous KLF4 and Krox20 are dispensable for adipogenesis in culture and brown adipose tissue development in mice...
October 24, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27753270/obesity-modifies-bone-marrow-microenvironment-and-directs-bone-marrow-mesenchymal-cells-to-adipogenesis
#18
Simone Vargas da Silva, Mariana Renovato-Martins, Cristiane Ribeiro-Pereira, Marta Citelli, Christina Barja-Fidalgo
OBJECTIVE: To investigate the role of obesity on the bone marrow microenvironment and evaluate its possible impact on the adipogenic potential of mesenchymal stem cells (MSC). METHODS: C57BL/6 male mice were fed with a high-fat diet (HFD) for 10 weeks. Femurs and tibiae were collected, and bone marrow mesenchymal stem cells (BM-MSC) were isolated and analyzed for proliferative potential, immunophenotype, and expression of adipogenesis markers. Their capacity to produce extracellular matrix proteins and proinflammatory cytokines in vitro was also evaluated...
October 18, 2016: Obesity
https://www.readbyqxmd.com/read/27742678/mir-181a-5p-regulates-3t3-l1-cell-adipogenesis-by-targeting-smad7-and-tcf7l2
#19
Dan Ouyang, Lifeng Xu, Lihua Zhang, Dongguang Guo, Xiaotong Tan, Xiaofang Yu, Junjie Qi, Yaqiong Ye, Qihong Liu, Yongjiang Ma, Yugu Li
MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation...
October 14, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27735943/trail-tnf-related-apoptosis-inducing-ligand-inhibits-human-adipocyte-differentiation-via-caspase-mediated-downregulation-of-adipogenic-transcription-factors
#20
Verena Zoller, Jan-Bernd Funcke, Michaela Keuper, Muad Abd El Hay, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
Tumor necrosis factor-α (TNFα) and other ligands of the TNF superfamily are potent regulators of adipose tissue metabolism and play a crucial role in the obesity-induced inflammation of adipose tissue. Adipose tissue expression levels of TRAIL (TNF-related apoptosis-inducing ligand) and its receptor were shown to be upregulated by overfeeding and decreased by fasting in mice. In the present study we aimed to elucidate the impact of TRAIL on adipogenesis. To this end, human Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes as well as stromal-vascular cells isolated from human white adipose tissue were used as model systems...
October 13, 2016: Cell Death & Disease
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