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preadipocyte AND differentiation

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https://www.readbyqxmd.com/read/28527661/modulation-of-adipocyte-function-by-the-tgf-%C3%AE-family
#1
Yuhang Qiao, Shozo Tomonaga, Masashi Suenaga, Tohru Matsui, Masayuki Funaba
Members of the transforming growth factor-β (TGF-β) family are known to regulate the commitment of mesenchymal stem cells to an adipocyte lineage and the differentiation of preadipocytes to adipocytes. However, the roles of TGF-β family members in mature adipocytes are not known. The present study explored the modulation of cell function in 3T3-L1 adipocytes treated with the TGF-β family members TGF-β1, activin A, activin B, bone morphogenetic protein (BMP) 4, and BMP7. These TGF-β family members modulated the expression of some adipokines; activin A upregulated leptin expression, and activin B downregulated the expression of plasminogen activator inhibitor-1...
May 17, 2017: Cytokine
https://www.readbyqxmd.com/read/28526050/dbcamp-regulates-adipogenesis-in-sheep-inguinal-preadipocytes
#2
Delin Kong, Jingjing Cui, Juncai Fu
BACKGROUND: The cyclic adenosine-monophosphate (cAMP) pathway is generally recognized as one of the essential pathways for the adipose conversion of rodent preadipocytes in vitro. However, divergent effects of cAMP on adipocyte differentiation have also been reported. Since there is very little data on non-rodent preadipose cells, the aim of the present work was to analyze the effects of one classic activator (dbcAMP) of the cAMP pathway on the proliferation and differentiation of sheep preadipocytes grown...
May 19, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28501567/depletion-of-jmjd1c-impairs-adipogenesis-in-murine-3t3-l1-cells
#3
Florian Buerger, Silvana Müller, Nadja Ney, Juliane Weiner, John T Heiker, Sonja Kallendrusch, Peter Kovacs, Dorit Schleinitz, Joachim Thiery, Sonja C Stadler, Ralph Burkhardt
Differentiation of adipocytes is a highly regulated process modulated by multiple transcriptional co-activators and co-repressors. JMJD1C belongs to the family of jumonji C (jmjC) domain-containing histone demethylases and was originally described as a ligand-dependent co-activator of thyroid hormone and androgen receptors. Here, we explored the potential role of Jmjd1c in white adipocyte differentiation. To investigate the relevance of Jmjd1c in adipogenesis, murine 3T3-L1 preadipocyte cells with transient knock-down of Jmjd1c (3T3_Jmjd1c) were generated...
May 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28499918/cul3-neddylation-is-crucial-for-gradual-lipid-droplet-formation-during-adipogenesis
#4
Dawadschargal Dubiel, Willem Bintig, Thilo Kähne, Wolfgang Dubiel, Michael Naumann
Cullin 3 (Cul3) belongs to the family of cullins (Cul1-7) providing the scaffold for cullin-RING ubiquitin (Ub) ligases (CRLs), which are activated by neddylation and represent essential E3 ligases of the Ub proteasome system. During adipogenic differentiation neddylated Cul3 accumulates in LiSa-2 preadipocytes. Downregulation of Cul3 and inhibition of neddylation by MLN4924 blocks the formation of lipid droplets (LDs), the lipid storage organelles and markers of adipogenesis. Neddylation of Cul3 coincides with an increase of Rab18, a GTPase associated with LDs...
May 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28496206/wisp1-ccn4-inhibits-adipocyte-differentiation-through-repression-of-ppar%C3%AE-activity
#5
Nathalie Ferrand, Véronique Béreziat, Marthe Moldes, Maurice Zaoui, Annette K Larsen, Michèle Sabbah
WISP1 (Wnt1-inducible signaling pathway protein-1, also known as CCN4) is a member of the CCN family able to mediate cell growth, transformation and survival in a tissue-specific manner. Here, we report that WISP1 expression was highly increased in preadipocytes and decreased during adipocyte differentiation. Moreover, we observed an increase in WISP1 gene expression in adipose tissue from both diet-induced and leptin-deficient ob/ob obese mice, suggesting that WISP1 could be involved in the pathophysiological onset of obesity...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28494459/influence-of-bovine-serum-lipids-and-fetal-bovine-serum-on-the-expression-of-cell-surface-markers-in-cultured-bovine-preadipocytes
#6
Mansur A Sandhu, Sandra Jurek, Susanne Trappe, Martin Kolisek, Gerhard Sponder, Jörg R Aschenbach
To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4)...
May 12, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28486516/immortalization-of-chicken-preadipocytes-by-retroviral-transduction-of-chicken-tert-and-tr
#7
Wei Wang, Tianmu Zhang, Chunyan Wu, Shanshan Wang, Yuxiang Wang, Hui Li, Ning Wang
The chicken is an important agricultural animal and model for developmental biology, immunology and virology. Excess fat accumulation continues to be a serious problem for the chicken industry. However, chicken adipogenesis and obesity have not been well investigated, because no chicken preadipocyte cell lines have been generated thus far. Here, we successfully generated two immortalized chicken preadipocyte cell lines through transduction of either chicken telomerase reverse transcriptase (chTERT) alone or in combination with chicken telomerase RNA (chTR)...
2017: PloS One
https://www.readbyqxmd.com/read/28484500/pingmu-decoction-induces-orbital-preadipocytes-apoptosis-in-vitro
#8
Yali Zhang, Hong Li, Long Gao, Xia Zhang, RuiFang Xie
Pingmu Decoction is the Traditional Chinese Medicine which has treated Graves' Ophthalmopathy (GO) in the inactive stage for more than ten years. This study was to explore the mechanism of Pingmu Decoction of inhibiting preadipocytes in GO patients from differentiating into mature adipocytes. Human orbital preadipocytes were isolated and cultured through tissue explant method. Orbital preadipocytes were induced into mature adipocytes. The medicinal serum was prepared from rats. The cells were treated with medicinal serum which were divided into three groups, low dose group (5%), medium dose group (10%), and high dose group (20%)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28483479/sulfuretin-has-therapeutic-activity-against-acquired-lymphedema-by-reducing-adipogenesis
#9
Kangsan Roh, Suji Kim, Hee Kang, Jin-Mo Ku, Kye Won Park, Sukchan Lee
Acquired lymphedema is a pathological condition associated with lymphatic dysfunction caused by surgical treatments for cancer. Although global estimates of the prevalence of acquired lymphedema have been rising, there are currently no effective therapeutics available. Since adipose tissue accumulation is a clinical hallmark of lymphedema, we hypothesized that regulation of adipogenesis in lymphedematous tissue could be used as a therapeutic intervention against lymphedema. Toward this, we investigated the possibility of anti-adipogenic 30% ethanol Rhus verniciflua Stokes (RVS) extract as a potential lymphedema treatment...
May 5, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28475875/dynamic-rewiring-of-promoter-anchored-chromatin-loops-during-adipocyte-differentiation
#10
Rasmus Siersbæk, Jesper Grud Skat Madsen, Biola Maria Javierre, Ronni Nielsen, Emilie Kristine Bagge, Jonathan Cairns, Steven William Wingett, Sofie Traynor, Mikhail Spivakov, Peter Fraser, Susanne Mandrup
Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstrate a rapid reorganization of promoter-anchored chromatin loops within 4 hr after inducing differentiation of 3T3-L1 preadipocytes. The establishment of new promoter-enhancer loops is tightly coupled to activation of poised (histone H3 lysine 4 mono- and dimethylated) enhancers, as evidenced by the acquisition of histone H3 lysine 27 acetylation and the binding of MED1, SMC1, and P300 proteins to these regions, as well as to activation of target genes...
May 4, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28475114/melatonin-and-vitamin-d-interfere-with-the-adipogenic-fate-of-adipose-derived-stem-cells
#11
Valentina Basoli, Sara Santaniello, Sara Cruciani, Giorgio Carlo Ginesu, Maria Laura Cossu, Alessandro Palmerio Delitala, Pier Andrea Serra, Carlo Ventura, Margherita Maioli
Adipose-derived stem cells (ADSCs) represent one of the cellular populations resident in adipose tissue. They can be recruited under certain stimuli and committed to become preadipocytes, and then mature adipocytes. Controlling stem cell differentiation towards the adipogenic phenotype could have a great impact on future drug development aimed at counteracting fat depots. Stem cell commitment can be influenced by different molecules, such as melatonin, which we have previously shown to be an osteogenic inducer...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28472935/gene-expression-profile-during-proliferation-and-differentiation-of-rainbow-trout-adipocyte-precursor-cells
#12
Marta Bou, Jerôme Montfort, Aurélie Le Cam, Cécile Rallière, Véronique Lebret, Jean-Charles Gabillard, Claudine Weil, Joaquim Gutiérrez, Pierre-Yves Rescan, Encarnación Capilla, Isabel Navarro
BACKGROUND: Excessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray. RESULTS: Our analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation...
May 4, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28472098/t1r3-homomeric-sweet-taste-receptor-regulates-adipogenesis-through-g%C3%AE-s-mediated-microtubules-disassembly-and-rho-activation-in-3t3-l1-cells
#13
Yosuke Masubuchi, Yuko Nakagawa, Johan Medina, Masahiro Nagasawa, Itaru Kojima, Mark M Rasenick, Takeshi Inagaki, Hiroshi Shibata
We previously reported that 3T3-L1 cells express a functional sweet taste receptor possibly as a T1R3 homomer that is coupled to Gs and negatively regulates adipogenesis by a Gαs-mediated but cAMP-independent mechanism. Here, we show that stimulation of this receptor with sucralose or saccharin induced disassembly of the microtubules in 3T3-L1 preadipocytes, which was attenuated by overexpression of the dominant-negative mutant of Gαs (Gαs-G226A). In contrast, overexpression of the constitutively active mutant of Gαs (Gαs-Q227L) as well as treatment with cholera toxin or isoproterenol but not with forskolin caused disassembly of the microtubules...
2017: PloS One
https://www.readbyqxmd.com/read/28469599/the-role-of-thyrotropin-receptor-activation-in-adipogenesis-and-modulation-of-fat-phenotype
#14
Mohd Shazli Draman, Michael Stechman, David Scott-Coombes, Colin M Dayan, Dafydd Aled Rees, Marian Ludgate, Lei Zhang
Evidence from clinical and experimental data suggests that thyrotropin receptor (TSHR) signaling is involved in energy expenditure through its impact on white adipose tissue (WAT) and brown adipose tissue (BAT). TSHR expression increases during mesenchymal stem cell (MSC) differentiation into fat. We hypothesize that TSHR activation [TSHR*, elevated thyroid-stimulating hormone, thyroid-stimulating antibodies (TSAB), or activating mutation] influences MSC differentiation, which contributes to body composition changes seen in hypothyroidism or Graves' disease (GD)...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28469146/optical-visualisation-of-thermogenesis-in-stimulated-single-cell-brown-adipocytes
#15
Rókus Kriszt, Satoshi Arai, Hideki Itoh, Michelle H Lee, Anna G Goralczyk, Xiu Min Ang, Aaron M Cypess, Andrew P White, Farnaz Shamsi, Ruidan Xue, Jung Yeol Lee, Sung-Chan Lee, Yanyan Hou, Tetsuya Kitaguchi, Thankiah Sudhaharan, Shin'ichi Ishiwata, E Birgitte Lane, Young-Tae Chang, Yu-Hua Tseng, Madoka Suzuki, Michael Raghunath
The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465089/adipocyte-lipid-storage-and-adipokine-production-are-modulated-by-lipoxygenase-derived-oxylipins-generated-from-18-carbon-fatty-acids
#16
Peter Zahradka, Shannon Neumann, Harold M Aukema, Carla G Taylor
Generation of oxylipins (oxygenated metabolites of fatty acids) by lipoxygenases may be responsible for the beneficial effects of 20- and 22-carbon n-3 fatty acids on adipose tissue dysfunction in obesity, but the potential actions of oxylipins derived from 18-carbon fatty acids, which are generally at higher levels in the diet, are unknown. We therefore compared the effects of select lipoxygenase-derived oxylipins produced from α-linolenic acid (ALA, C18:3 n-3), linoleic acid (LA, C18:2 n-6), and arachidonic acid (AA, C20:4 n-6) on key adipocyte functions that are altered in obesity...
April 29, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28464422/recombinant-ostreolysin-induces-brown-fat-like-phenotype-in-hib-1b-cells
#17
Tom Oren, Lili Nimri, Einav Yehuda-Shnaidman, Katy Staikin, Yitzhak Hadar, Assaf Friedler, Hadar Amartely, Michal Slutzki, Antonella Di Pizio, Masha Y Niv, Irena Peri, Lutz Graeve, Betty Schwartz
SCOPE: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes...
May 2, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28463991/selective-effect-of-phosphatidylcholine-on-the-lysis-of-adipocytes
#18
Ji-Young Kim, Min-Seo Kwon, Junghyun Son, Sang-Wook Kang, Youngsup Song
Obesity, a serious health risk factor, is often associated with depression and negatively affects many aspects of life. Injection of a formula comprising phosphatidylcholine (PPC) and deoxycholate (DC) has emerged as an alternative to liposuction in the reduction of local fat deposits. However, the formula component mainly responsible for this effect and the mechanism behind the actions of the components with respect to fat reduction are unknown. Here, we investigate the specific effects of PPC and DC on adipocyte viability...
2017: PloS One
https://www.readbyqxmd.com/read/28452592/a-cellular-model-for-the-investigation-of-depot-specific-human-adipocyte-biology
#19
Marijana Todorčević, Catriona Hilton, Catriona McNeil, Constantinos Christodoulides, Leanne Hodson, Fredrik Karpe, Katherine E Pinnick
Upper-body adiposity is associated with increased metabolic disease risk, while lower-body adiposity is paradoxically protective. Efforts to understand the underlying mechanisms require appropriate and reproducible in vitro culture models. We have therefore generated immortalised (im) human preadipocyte (PAD) cell lines derived from paired subcutaneous abdominal and gluteal adipose tissue. These cell lines, denoted imAPAD and imGPAD display enhanced proliferation and robust adipogenic capacities. Differentiated imAPAD and imGPAD adipocytes synthesize triglycerides de novo and respond lipolytically to catecholamine-stimulation...
January 2, 2017: Adipocyte
https://www.readbyqxmd.com/read/28449555/capsanthin-inhibits-both-adipogenesis-in-3t3-l1-preadipocytes-and-weight-gain-in-high-fat-diet-induced-obese-mice
#20
Sung Jun Jo, Jeung Won Kim, Hye Ok Choi, Jung Hwan Kim, Hyung Joong Kim, Sun Hee Woo, Byung Hoon Han
Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis (IC₅₀; 2.5 μM) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes (ED₅₀; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-β₂-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss...
May 1, 2017: Biomolecules & Therapeutics
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