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preadipocyte AND differentiation

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https://www.readbyqxmd.com/read/29140299/pdgfr%C3%AE-regulated-by-mir-34a-and-foxo1-promotes-adipogenesis-in-porcine-intramuscular-preadipocytes-through-erk-signaling-pathway
#1
Yun-Mei Sun, Jin Qin, Shu-Ge Liu, Rui Cai, Xiao-Chang Chen, Xiang-Ming Wang, Wei-Jun Pang
Suitable intramuscular fat (IMF) content improves porcine meat quality. The vital genes regulating IMF deposition are necessary for the selection and breeding of an IMF trait. However, the effect and mechanism of PDGFRα on IMF deposition are still unclear. Here, PDGFRα is moderately expressed in porcine longissimus dorsi muscle (LD), whereas it highly expressed in white adipose tissue (WAT). Moreover, PDGFRα-positive cells were located in the gaps of LD fibers which there were IMF adipocytes. Compared with 180-day-old and lean-type pigs, the levels of PDGFRα were much higher in one-day-old and fat-type pigs...
November 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29138673/the-beneficial-effects-of-quercetin-curcumin-and-resveratrol-in-obesity
#2
REVIEW
Yueshui Zhao, Bo Chen, Jing Shen, Lin Wan, Yinxin Zhu, Tao Yi, Zhangang Xiao
Over the past two decades, obesity has been one of the major public health concerns in most countries. In the search for new molecules that could be used for the treatment of obesity, good perspectives have been opened up for polyphenols, a class of natural bioactive phytochemicals. Experimental and limited clinical trial evidence supports that some polyphenols such as quercetin, curcumin, and resveratrol have potential benefit functions on obesity treatment. This brief review focuses on the main functions of the above-named polyphenols on adipose tissue...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29138456/prep1-prevents-premature-adipogenesis-of-mesenchymal-progenitors
#3
Giorgia Maroni, Vsevolod A Tkachuk, Alexander Egorov, Marco J Morelli, Raffaele Luongo, Elena Levantini, Francesco Blasi, Maria Cristina Magli, Dmitry Penkov
Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) in both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and in vitro 3T3-L1 preadipocytes significantly increases their adipogenic differentiation ability. Prep1 acts at a stage preceding the activation of the differentiation machinery because its DR makes cells more prone to adipogenic differentiation even in the absence of the adipogenic inducers...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137982/adipose-tissue-complement-factor-b-promotes-adipocyte-maturation
#4
Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Akiko Yamashita, Mitsudai Tsuruta, Shoichiro Nagasaka, Ataru Taniguchi, Mitsuo Fukushima, Naoya Watanabe, Fusanori Nishimura
OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137239/transcriptome-profiling-in-preadipocytes-identifies-long-noncoding-rnas-as-sam68-targets
#5
Naomi Li, Steven Hébert, Jingwen Song, Claudia L Kleinman, Stéphane Richard
The KH-type RNA binding protein Sam68 is required for adipogenesis. We have previously shown that Sam68-deficient mice have a lean phenotype and are protected against dietary-induced obesity due to defects in mTOR and S6K1 alternative splicing. Herein we profiled the transcriptome of Sam68 wild type and deficient 3T3-L1 mouse preadipocytes. We identified 652 protein-coding genes and 9 ncRNAs that were significantly altered with the loss of Sam68. As expected, downregulated genes were significantly associated with GO terms linked to cell migration, motility, and fat cell differentiation, while upregulated genes were mostly associated with GO terms linked to neurogenesis...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29133280/mat2a-promotes-porcine-adipogenesis-by-mediating-h3k27me3-at-wnt10b-locus-and-repressing-wnt-%C3%AE-catenin-signaling
#6
Cunzhen Zhao, Haigang Wu, Naren Qimuge, Weijun Pang, Xiao Li, Guiyan Chu, Gongshe Yang
Methionine adenosyltransferase (MAT) is a critical biological enzyme and that can catalyze L-met and ATP to form S-adenosylmethionine (SAM), which is acted as a biological methyl donor in transmethylation reactions involving histone methylation. However, the regulatory effect of methionine adenosyltransferase2A (MAT2A) and its associated methyltransferase activity on adipogenesis is still unclear. In this study, we investigate the effect of MAT2A on adipogenesis and its potential mechanism on histone methylation during porcine preadipocyte differentiation...
November 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29125119/research-progress-of-the-differentiation-of-3t3-l1-preadipocytes-into-mature-adipocytes
#7
Xiu Liu, Xi-Nong Liu, Tian-Jia Li, Bao Liu
Along with the increase in the prevalence of lipid metabolism disorders,in vitro research on the adipocytes has been a hot topic in recent years. 3T3-L1 preadipocyte line is one of the most commonly used cell line for establishing adipocytes models. However,for 3T3-L1 cell lines,the traditional method,known also as the "Cocktail" method,have disadvantages including low transformation rate and heterogeneous conversion. Therefore,many studies aimed to improving the induction method by changing the time of inducers or adding other new drugs have been performed(known as the improved method)...
October 30, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29118419/melatonin-promotes-triacylglycerol-accumulation-via-mt2-receptor-during-differentiation-in-bovine-intramuscular-preadipocytes
#8
Wucai Yang, Keqiong Tang, Yaning Wang, Yingying Zhang, Linsen Zan
Melatonin (N-acetyl-5-methoxytryptamine) is a derivative of tryptophan which is produced and secreted mainly by the pineal gland and regulates a variety of important central and peripheral actions. To examine the potential effects of melatonin on the proliferation and differentiation of bovine intramuscular preadipocytes (BIPs), BIPs were incubated with different concentrations of melatonin. Melatonin supplementation at 1 mM significantly increased peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein (C/EBP) β, and C/EBPα expression and promoted the differentiation of BIPs into adipocytes with large lipid droplets and high cellular triacylglycerol (TAG) levels...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115635/a-potential-role-for-hepcidin-in-obesity-driven-colorectal-tumourigenesis
#9
Elisabeth Phillips, Richard D Horniblow, Vikki Poole, Matthew Bedford, Douglas G Ward, Amanda J Kirkham, Jeremy Tomlinson, Tariq H Iqbal, Chris Tselepis
The obesity epidemic is associated with increases in the incidence of several types of cancer, including colorectal cancer, and is associated with poor outcomes for patients. Adipose tissue is considered biologically active and represents a plausible link between cancer and obesity due to the many factors that it secretes. In the present study, human adipose tissue was cultured in vitro and predifferentiated adipocyte secretome [preadipocyte (PAS)] and differentiated adipocyte secretome (DAS) were collected...
October 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29115433/adiponectin-promotes-preadipocyte-differentiation-via-the-ppar%C3%AE-pathway
#10
Wenkai Yang, Chunli Yang, Jun Luo, Yutao Wei, Wenying Wang, Yingmei Zhong
According to the results of a preliminary study, it was hypothesized that the effects of adiponectin (APN) on the improvement of atherosclerosis may be associated with adipocyte differentiation and peroxisome proliferator‑activated receptor γ (PPARγ). The present study simulated the inflammatory environment of epicardial adipose tissue by stimulating mature adipocytes with lipopolysaccharide (LPS); subsequently, the differentiation of 3T3‑L1 preadipocytes was observed. 3T3‑L1 preadipocytes were infected with an adenovirus containing the human adiponectin gene apM1 (Ad‑apM1) and were co‑cultured with mature adipocytes stimulated with LPS...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29113429/endophilin-a2-a-potential-link-to-adiposity-and-beyond
#11
Assim A Alfadda, Reem M Sallam, Rukhsana Gul, Injae Hwang, Sojeong Ka
Adipose tissue plays a central role in regulating dynamic crosstalk between tissues and organs. A detailed description of molecules that are differentially expressed upon changes in adipose tissue mass is expected to increase our understanding of the molecular mechanisms that underlie obesity and related metabolic co-morbidities. Our previous studies suggest a possible link between endophilins (SH3Grb2 proteins) and changes in body weight. To explore this further, we sought to assess the distribution of endophilin A2 (EA2) in human adipose tissue and experimental animals...
November 6, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29095919/raldh1-promotes-adiposity-during-adolescence-independently-of-retinal-signaling
#12
Di Yang, Charles R Krois, Priscilla Huang, Jinshan Wang, Jin Min, Hong Sik Yoo, Yinghua Deng, Joseph L Napoli
All-trans-retinoic acid (RA) inhibits adipogenesis in established preadipocyte cell lines. Dosing pharmacological amounts of RA reduces weight gain in mice fed a high-fat diet, i.e. counteracts diet-induced obesity (DIO). The aldehyde dehydrogenase Raldh1 (Aldh1a1) functions as one of three enzymes that converts the retinol metabolite retinal into RA, and one of many proteins that contribute to RA homeostasis. Female Raldh1-ablated mice resist DIO. This phenotype contrasts with ablations of other enzymes and binding-proteins that maintain RA homeostasis, which gain adiposity...
2017: PloS One
https://www.readbyqxmd.com/read/29094436/low-level-perfluorooctanoic-acid-enhances-3%C3%A2-t3-l1-preadipocyte-differentiation-via-altering-peroxisome-proliferator-activated-receptor-gamma-expression-and-its-promoter-dna-methylation
#13
Yue Ma, Jie Yang, Yanjian Wan, Yang Peng, Shuai Ding, Yuanyuan Li, Bing Xu, Xi Chen, Wei Xia, Yuebin Ke, Shunqing Xu
Recent studies suggest that perfluorooctanoic acid (PFOA) can play a role in the development of obesity; however, the associated mechanisms are poorly understood. We investigated how PFOA exposure affected the differentiation of 3 T3-L1 preadipocytes and the associated transcriptional and epigenetic mechanisms. Cells treated with different doses of PFOA (ranging from 0.01 to 100 μg ml(-1) ) were assessed for proliferation, differentiation and triglyceride accumulation. The gene expression levels of peroxisome proliferator activated receptor gamma (PPARγ) and its target genes were measured...
November 2, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29093466/scara5-plays-a-critical-role-in-the-commitment-of-mesenchymal-stem-cells-to-adipogenesis
#14
Hyemin Lee, Yoo Jeong Lee, Hyeonjin Choi, Jo Woon Seok, Bo Kyung Yoon, Daeun Kim, Ji Yoon Han, Yoseob Lee, Hyo Jung Kim, Jae-Woo Kim
Mesenchymal stem cells have the capacity to give rise to multiple cell types, such as adipocytes, osteoblasts, chondrocytes, and myocytes. However, the molecular events responsible for the lineage specification and differentiation of mesenchymal stem cells remain unclear. Using gene expression profile studies, we determined that Scavenger receptor class A, member 5 (SCARA5) is a novel mediator of adipocyte commitment. SCARA5 was expressed at a higher level in committed A33 preadipocyte cells compared to C3H10T1/2 pluripotent stem cells...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29082702/-effects-of-mori-folium-extract-on-diet-induced-obesity-mechanism-in-rats
#15
Wen Wu, Kai-Lun Liang, Bo Chen, Jie Su, Su-Hong Chen, Gui-Yuan Lyu
To study the anti-obesity effect of Mori Folium extract on diet-induced obesity(DIO) and to explore the preliminary mechanism in rats. DIO rat models were established by high glucose and high fat diet for 8 weeks. Then high(10 mg•kg⁻¹) and low(5 mg•kg⁻¹) does Mori Folium extracts were given by intragastric administration for 13 weeks. After the last administration, their body weight, 24 h food intake, water intake, Lee's index, liver/body mass index, and fat/body mass index were determined. The levels of lipoprotein lipase(LPL), CCAAT/enhancer-binding protein alpha(C/EBPα) and peroxisome proliferator-activated receptor gamma(PPARγ) were measured by enzyme-linked immunosorbent assay(ELISA)...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29073274/transcriptome-analysis-of-mrna-and-micrornas-in-intramuscular-fat-tissues-of-castrated-and-intact-male-chinese-qinchuan-cattle
#16
Ying-Ying Zhang, Hong-Bao Wang, Ya-Ning Wang, Hong-Cheng Wang, Song Zhang, Jie-Yun Hong, Hong-Fang Guo, Dai Chen, Yang Yang, Lin-Sen Zan
Intramuscular fat (IMF) is known to enhance beef palatability and can be markedly increased by castration. However, there is little understanding of the molecular mechanism underlying the IMF deposition after castration of beef cattle. We hypothesize that genetic regulators function differently in IMF from bulls and steers. Therefore, after detecting serum testosterone and lipid parameter, as well as the contents of IMF at 6, 12, 18 and 24 months, we have investigated differentially expressed (DE) microRNAs (miRNAs) and mRNAs in IMF of bulls and steers at 24 months of age in Qinchuan cattle using next-generation sequencing, and then explored the possible biopathways regulating IMF deposition...
2017: PloS One
https://www.readbyqxmd.com/read/29060669/electrical-bioimpedance-spectroscopy-as-biosensor-technique-to-identify-cells-lineages-and-cell-differentiation-process
#17
C I Guerrero-Robles, G J Vazquez-Zapien, M M Mata-Miranda, J E Noriega-Gonzalez, C A Gonzalez-Diaz
The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29059354/inflammation-and-fibrosis-in-perirenal-adipose-tissue-of-patients-with-aldosterone-producing-adenoma
#18
Chunyan Wu, Huijian Zhang, Jiajun Zhang, Cuihua Xie, Cunxia Fan, Hongbin Zhang, Peng Wu, Qiang Wei, Wanlong Tan, Lingling Xu, Ling Wang, Yaoming Xue, Meiping Guan
The prevalence of primary aldosteronism is much higher than previously thought. Recent studies have shown that primary aldosteronism is related to a higher risk of cardiovascular events. However, the underlying mechanism is not yet clear. Here we investigate the characteristics, including inflammation, fibrosis and adipokine expression, of adipose tissues from different deposits in patients with aldosterone-producing adenoma (APA). Inflammation and fibrosis changes were evaluated in perirenal and subcutaneous adipose tissues obtained from patients with APA (n=16), normotension (NT; n=10) and essential hypertension (EH; n=5) undergoing laparoscopic surgery...
October 19, 2017: Endocrinology
https://www.readbyqxmd.com/read/29058774/mesoderm-specific-transcript-localization-in-the-er-and-er-lipid-droplet-interface-supports-a-role-in-adipocyte-hypertrophy
#19
Igor Prudovsky, Rea P Anunciado-Koza, Chester G Jacobs, Doreen Kacer, Matthew E Siviski, Robert A Koza
Highly variable expression of mesoderm specific transcript (Mest) in adipose tissue among genetically homogeneous mice fed an obesogenic diet, and its positive association with fat mass expansion, suggests that Mest is an epigenetic determinant for the development of obesity. Although the mechanisms by which MEST augments fat accumulation in adipocytes have not been elucidated, it has sequence homology and catalytic peptide motifs which suggests that it functions as an epoxide hydrolase or as a glycerol- or acylglycerol-3-phosphate acyltransferase...
October 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29040876/species-specific-control-of-hepatocyte-growth-factor-expression-and-production-in-adipocytes-in-a-differentiation-dependent-manner
#20
D Yamaji, M M Soliman, A Kamikawa, T Ito, M M Ahmed, Y Okamatsu-Ogura, M Saito, K Kimura
Hepatocyte growth factor (HGF) is a mesenchymal cell-derived factor that regulates cell growth, cell motility, and morphogenesis. Since there are conflicting reports on HGF-producing cells, we herein examined HGF activity in conditioned medium (CM) of bovine and mouse preadipocytes before and after adipogenic differentiation. CM of bovine adipocytes and mouse preadipocytes induced the morphogenesis of mammary epithelial cells that was inhibited by an NK4 HGF antagonist, whereas CM of bovine preadipocytes and mouse adipocytes did not...
September 15, 2017: Domestic Animal Endocrinology
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