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preadipocyte AND differentiation

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https://www.readbyqxmd.com/read/28627722/pterostilbene-inhibits-lipogenic-activity-similar-to-resveratrol-or-caffeine-but-differently-modulates-lipolysis-in-adipocytes
#1
Saioa Gomez-Zorita, Chloé Belles, Anaïs Briot, Alfredo Fernández-Quintela, Maria P Portillo, Christian Carpéné
The anti-obesity effects of resveratrol shown in rodents are not transposed into an efficient therapy of human obesity. Consequently, the search for molecules mimicking or surpassing resveratrol actions is ongoing. The natural phenolic compound pterostilbene exhibits beneficial health effects and has the capacity to limit fat mass in animal models. In this study, we tested whether pterostilbene modulates triacylglycerol accumulation/breakdown. Prolonged exposure to pterostilbene or resveratrol inhibited adipocyte differentiation in 3T3-F442A preadipocytes...
June 19, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28614778/the-human-immunodeficiency-virus-hiv1-protease-inhibitor-sanquinavir-activates-autophagy-and-removes-lipids-deposited-in-lipid-droplets
#2
A Polus, M Bociaga-Jasik, U Czech, J Goralska, U Cialowicz, M Chojnacka, M Polus, K Jurowski, A Dembinska-Kiec
Reduction in mortality and increased average life span of the human immunodeficiency virus (HIV)-infected patients treated with antiretroviral therapy (ART) are associated with the risk of unwanted effects, such as insulin resistance and dyslipidemia with cardiovascular complications. Antiretroviral therapy may also be associated with lipodystrophy characterized as peripheral lipoatrophy with central fat accumulation. Understanding the molecular mechanisms of lipodystrophy caused by ART is important for therapeutic strategy and the prediction of side-effects...
April 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28613919/hypercapnia-accelerates-adipogenesis-a-novel-role-of-high-co2-in-exacerbating-obesity
#3
Ryota Kikuchi, Takao Tsuji, Osamu Watanabe, Kazuhiro Yamaguchi, Kinya Furukawa, Hiroyuki Nakamura, Kazutetsu Aoshiba
Obesity is a major risk factor for the development of obstructive sleep apnea (OSA) and obesity hypoventilation syndrome (OHS), which manifest as intermittent hypercapnia and sustained plus intermittent hypercapnia, respectively. In this study, we investigated whether CO2 affects adipocyte differentiation (adipogenesis) and maturation (hypertrophy). Human visceral or subcutaneous preadipocytes were grown to confluence and then induced to differentiate to adipocytes under hypocapnia, normocapnia, and hypercapnia with or without hypoxia...
June 14, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28611394/identification-of-the-ectoenzyme-cd38-as-a-marker-of-committed-preadipocytes
#4
A Carrière, Y Jeanson, J-A Côté, C Dromard, A Galinier, S Menzel, C Barreau, S Dupuis-Coronas, E Arnaud, A Girousse, V Cuminetti, J Paupert, B Cousin, C Sengenes, F Koch-Nolte, A Tchernof, L Casteilla
BACKGROUND/OBJECTIVES: Characterization of the adipocyte cellular lineage is required for a better understanding of white adipose tissue homeostasis and expansion. While several studies have focused on the phenotype of the most immature adipocyte progenitors, very few tools exist to identify committed cells. In haematopoiesis, the CD38 ectoenzyme is largely used to delineate various stages of stem cell lineage commitment. We hypothesize that this marker could be used to identify committed preadipocytes...
June 14, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28606512/heme-oxygenase-1-mediates-anti-adipogenesis-effect-of-raspberry-ketone-in-3t3-l1-cells
#5
Yung-Chieh Tsai, Bo-Cheng Yang, Wen-Huang Peng, Yen-Mei Lee, Mao-Hsiung Yen, Pao-Yun Cheng
BACKGROUND: Obesity is caused by excessive accumulation of body fat and is closely related to complex metabolic diseases. Raspberry ketone (RK), a major aromatic compound in red raspberry, was recently reported to possess anti-obesity effects. However, its mechanisms are unclear. AIM: Adipogenesis plays a critical role in obesity and, therefore, this study aimed to investigate the effect and mechanisms of action of RK on adipogenesis in 3T3-L1 preadipocytes. MATERIALS AND METHODS: 3T3-L1 preadipocytes were differentiated in medium containing insulin, dexamethasone, and 1-methyl-3-isobutylxanthine...
July 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28602994/fish-collagen-peptide-inhibits-the-adipogenic-differentiation-of-preadipocytes-and-ameliorates-obesity-in-high-fat-diet-fed-mice
#6
Eun Ji Lee, Jinwoo Hur, Sun Ah Ham, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Han Geuk Seo
Bioactivities of fish collagen peptide are now being elucidated in diverse biological systems. Here, we investigated the effect of fish collagen peptide on the adipogenic differentiation of 3T3-L1 preadipocytes and in obese mice fed a high fat diet (HFD). Subcritical water-hydrolyzed fish collagen peptide (SWFCP) significantly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes, which was accompanied by decreased expression of CCAAT-enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2) genes, key regulators of differentiation and maintenance of adipocytes...
June 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28595985/curcumin-inhibits-adipogenesis-induced-by-benzyl-butyl-phthalate-in-3t3-l1-cells
#7
Satoru Sakuma, Maki Sumida, Yukiko Endoh, Ayaka Kurita, Ayana Yamaguchi, Tomoki Watanabe, Tetsuya Kohda, Yui Tsukiyama, Yohko Fujimoto
Phthalates are a group of endocrine disrupting chemicals and may have contributed to the recent global obesity health crisis. Increased adipogenesis via the peroxisome proliferator-activated receptor γ (PPARγ)-CCAAT-enhancer binding protein α (C/EBPα) pathway could be one critical mechanism responsible for phthalate-induced weight gain. On the other hand, curcumin has been shown to inhibit adipogenesis in cells and animal models. The present study was undertaken to evaluate, for the first time, whether curcumin could reduce adipogenesis induced by benzyl butyl phthalate (BBP) via downregulation of the PPARγ-C/EBPα pathway...
June 10, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28585205/adipose-tissue-hypoxia-in-obesity-and-its-impact-on-preadipocytes-and-macrophages-hypoxia-hypothesis
#8
Atilla Engin
Obese subjects exhibit lower adipose tissue oxygen consumption in accordance with the lower adipose tissue blood flow. Thus, compared with lean subjects, obese subjects have 44% lower capillary density and 58% lower vascular endothelial growth factor (VEGF). The VEGF expression together with hypoxia-inducible transcription factor-1 (HIF-1) activity also requires phosphatidylinositol 3-kinase (PI3K)- and target of rapamycin (TOR)-mediated signaling. HIF-1alpha is an important signaling molecule for hypoxia to induce the inflammatory responses...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28582464/total-control-of-fat-cells-from-adipogenesis-to-apoptosis-using-a-xanthene-analog
#9
Ching-Hsuan Tung, Myung Shin Han, Jianjun Qi
Overcrowded adipocytes secrete excess adipokines and cytokines under stress, which results in a deregulated metabolism. This negative response to stress increases the possibility of obesity and several of its associated diseases, such as cancer and atherosclerosis. Therefore, a reduction in the number of adipocytes may be a rational strategy to relieve the undesired expansion of adipose tissue. A newly synthesized xanthene analog, MI-401, was found to have two distinct effects on the regulation of the adipocyte's life cycle...
2017: PloS One
https://www.readbyqxmd.com/read/28580300/resveratrol-attenuates-triglyceride-accumulation-associated-with-upregulation-of-sirt1-and-lipoprotein-lipase-in-3t3-l1-adipocytes
#10
Haruki Imamura, Daiji Nagayama, Noriko Ishihara, Syo Tanaka, Rena Watanabe, Yasuhiro Watanabe, Yuta Sato, Takashi Yamaguchi, Noriko Ban, Hidetoshi Kawana, Masahiro Ohira, Kei Endo, Atsuhito Saiki, Kohji Shirai, Ichiro Tatsuno
AIM: We aimed to investigate the effect of resveratrol (Rsv) on expression of genes regulating triglyceride (TG) accumulation and consumption in differentiated 3T3-L1 preadipocytes. METHODS: 3T3-L1 preadipocytes were cultured in DMEM supplemented with 10% fetal calf serum. Upon reaching confluence, cells were induced to differentiate for 4 days, cultured for 10 days for TG accumulation, and then incubated with Rsv (0, 25 or 50 μM) for 3 days. TG accumulation was analyzed by Oil Red-O staining...
September 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28580233/connecting-bone-and-fat-the-potential-role-for-sclerostin
#11
Heather Fairfield, Clifford J Rosen, Michaela R Reagan
Sclerostin (SOST), a protein secreted from mature osteocytes in response to mechanical unloading and other stimuli, inhibits the osteogenic Wnt/β-catenin pathway in mesenchymal stem cells (MSCs) impeding their ability to differentiate into mineralizing osteoblasts. PURPOSE: This review summarizes the crosstalk between adipose tissue and bone. It also reviews the origin, regulation, and role of SOST in osteogenesis and brings attention to an emerging role of this protein in the regulation of adipogenesis...
June 2017: Current Molecular Biology Reports
https://www.readbyqxmd.com/read/28571817/development-of-orbital-adipose-derived-stem-cells-as-a-model-for-studying-the-formation-of-baggy-lower-eyelids
#12
Xi Chen, Guangpeng Liu
In old population, there is often a protrusion of the orbital fat pad underlying the skin in the lower eyelids, giving an aspect of palpable pouches. It is generally thought that orbital fat hyperplasia is the main contributing factor to the formation of baggy lower eyelids, and resection of excessive orbital fat pad is routinely performed during the eyelid cosmetic surgery. In our clinical study, however, it was revealed that the adipocytes in orbital fat tissue from older people became smaller compared to those from the young individuals...
June 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28566583/bergamottin-promotes-adipocyte-differentiation-and-inhibits-tumor-necrosis-factor-%C3%AE-induced-inflammatory-cytokines-induction-in-3t3-l1-cells
#13
Hideya Mizuno, Tomoko Hatano, Ayako Taketomi, Mami Kawabata, Toshikatsu Nakabayashi
 Nowadays, a lot of food ingredients are marketed as dietary supplements for health. Because the effectiveness and mechanisms of these compounds have not been fully characterized, they might have unknown functions. Therefore, we investigated the effect of several food ingredients (Bergamottin, Chrysin, L-Citrulline and β-Carotene) known as health foods on adipocyte differentiation by using 3T3-L1 preadipocytes. In this study, we found that Bergamottin, a furanocoumarin isolated from grapefruit juice, promotes adipocyte differentiation...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28564636/depot-specific-differences-in-fat-mass-expansion-in-wt-and-ob-ob-mice
#14
Xinxia Wang, Caihua Yu, Jie Feng, Jin Chen, Qin Jiang, Shihuan Kuang, Yizhen Wang
The study was designed to investigate the cellular mechanisms underlying the differential fat expansion in different fat depots in wild type (WT) and ob/ob (OB) mice. At 6 weeks old, no differences in fat mass were found between SAT and VAT in WT mice, while O-SAT showed significantly higher weight than that of O-VAT. The average adipocyte size of SAT (~ 4133.47 μm2) was smaller than that of VAT (~ 7438.91 μm2) in OB mice. O-SAT preadipocytes gained higher triglyceride contents and higher levels of PPARγ and C/EBPα than did O-VAT preadipocytes upon in vitro differentiation...
May 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28555033/annual-wormwood-leaf-inhibits-the-adipogenesis-of-3t3-l1-and-obesity-in-high-fat-diet-induced-obese-rats
#15
Yuno Song, Soo-Jung Lee, Sun-Hee Jang, Tae Hoon Kim, Hong-Duck Kim, Sung-Woo Kim, Chung-Kil Won, Jae-Hyeon Cho
Annual wormwood (AW) (Artemisia annua L.) has anti-malarial, anti-bacterial, anti-oxidant, anti-tumour, and anti-inflammatory activities. In the present study, we evaluated the effects of annual wormwood leaves (AWL) on adipocyte differentiation in 3T3-L1 cells and high-fat diet (HFD)-induced obese rats. 3T3-L1 adipocytes and HFD-induced obese rats were treated with AWL, and its effect on gene expression was analyzed using RT-PCR and Western blotting experiments. Treatment with AWL effectively prevented triglyceride accumulation during adipogenesis in a dose-dependent manner...
May 28, 2017: Nutrients
https://www.readbyqxmd.com/read/28552586/adipocyte-differentiation-is-regulated-by-mitochondrial-trifunctional-protein-%C3%AE-subunit-via-sirtuin-1
#16
Hong-Xia Liu, Yan-Mei Wang, Jian-Ping Hu, Li-Ying Huang, Ning-Yuan Fang
Mitochondrial trifunctional protein α-subunit (MTPα) is involved in the fatty acid β-oxidation (FAO) pathway. Two MTPα activities, 3-hydroxyacyl-CoA dehydrogenase and long-chain hydratase, have been linked with the occurrence and development of obesity and obesity-related disorders. These activities catalyze two steps in the FAO pathway (the second and third reactions). However, the role of MTPα in the pathogenesis of obesity has not been evaluated, and the functional role of MTPα in adipocyte differentiation has not been determined...
May 26, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28548969/transcriptome-profiling-in-preadipocytes-identifies-long-noncoding-rnas-as-sam68-targets
#17
Naomi Li, Steven Hébert, Jingwen Song, Claudia L Kleinman, Stéphane Richard
The KH-type RNA binding protein Sam68 is required for adipogenesis. We have previously shown that Sam68-deficient mice have a lean phenotype and are protected against dietary-induced obesity due to defects in mTOR and S6K1 alternative splicing. Herein we profiled the transcriptome of Sam68 wild type and deficient 3T3-L1 mouse preadipocytes. We identified 652 protein-coding genes and 9 ncRNAs that were significantly altered with the loss of Sam68. As expected, downregulated genes were significantly associated with GO terms linked to cell migration, motility, and fat cell differentiation, while upregulated genes were mostly associated with GO terms linked to neurogenesis...
May 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28539724/anti-differentiation-effect-of-b-d-seco-limonoids-of-swietenia-mahogani
#18
Heejung Yang, Mina Choi, Dong Young Lee, Sang Hyun Sung
BACKGROUND: Obesity is a pathological state caused by abnormal or excessive accumulation of fat. Swietenia mahogani JACQ., known as West Indian mahogany, is a medium-sized semi-evergreen tree belonging to Meliaceae. Their seeds are used in Indonesian folk medicine as a treatment for hypertension, diabetes, malaria, and it also has anti-feedant activities. The major components of S. mahogani are B, D-seco limonoids, a type of irregular triterpenes are well known. OBJECTIVE: We tried to find the bioactive components, which have the inhibitory activity on adipocyte differentiation from the seeds of S...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539051/a-new-diarylheptanoid-from-alpinia-officinarum-promotes-the-differentiation-of-3t3-l1-preadipocytes
#19
Xuguang Zhang, Xiaopo Zhang, Yong Wang, Feng Chen, Youbin Li, Yonghui Li, Yinfeng Tan, Jingwen Gong, Xia Zhong, Hailong Li, Junqing Zhang
A new diarylheptanoid, namely trans-(4R,5S)-epoxy-1,7-diphenyl-3-heptanone (1), and a new natural product, 7-(4″-hydroxy-3″-methoxyphenyl)-1-phenyl-hepta-4E,6E-dien-3-one (2), were obtained from the aqueous extract of Alpinia officinarum Hance, together with three other diarylheptanoids, 5-hydroxy-1,7-diphenyl-3-heptanone (3), 1,7-diphenyl-4E-en-3-heptanone (4) and 5-methoxy-1,7-diphenyl-3-heptanone (5). The structures were characterised mainly by analysing their physical data including IR, NMR and HRMS...
May 25, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28536637/ppar%C3%AE-agonist-through-the-terminal-differentiation-phase-is-essential-for-adipogenic-differentiation-of-fetal-ovine-preadipocytes
#20
Yong Pu, Almudena Veiga-Lopez
BACKGROUND: Although the 3T3-L1 preadipocyte cell line represents an informative model for in vitro adipogenesis research, primary cultured cells are often needed to understand particular human or animal metabolic phenotypes. As demonstrated by in vitro cultured preadipocytes from large mammalian species, primary cultured cells require specific adipogenic differentiation conditions different to that of the 3T3-L1 cell line. These conditions are also species-specific and require optimization steps...
2017: Cellular & Molecular Biology Letters
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