Avinash Waghray, Isha Monga, Brian Lin, Viral Shah, Michal Slyper, Bruno Giotti, Jiajie Xu, Julia Waldman, Danielle Dionne, Lan T Nguyen, Wendy Lou, Peiwen Cai, Eric Park, Christoph Muus, Jiawei Sun, Manalee V Surve, Lujia Cha Cha Yang, Orit Rozenblatt-Rosen, Toni M Dolerey, Srinivas Vinod Saladi, Alexander M Tsankov, Aviv Regev, Jayaraj Rajagopal
The human airway contains specialized rare epithelial cells whose roles in respiratory disease are not well understood. Ionocytes express the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), while chemosensory tuft cells express asthma-associated alarmins. However, surprisingly, exceedingly few mature tuft cells have been identified in human lung cell atlases despite the ready identification of rare ionocytes and neuroendocrine cells. To identify human rare cell progenitors and define their lineage relationship to mature tuft cells, we generated a deep lung cell atlas containing 311,748 single cell RNA-Seq (scRNA-seq) profiles from discrete anatomic sites along the large and small airways and lung lobes of explanted donor lungs that could not be used for organ transplantation...
November 29, 2023: bioRxiv