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Alison Obergrussberger, Krisztina Juhasz, Ulrich Thomas, Sonja Stölzle-Feix, Nadine Becker, Leo Dörr, Matthias Beckler, Corina Bot, Michael George, Niels Fertig
INTRODUCTION: While extracellular field potential (EFP) recordings using multi-electrode arrays (MEAs) are a well-established technique for monitoring changes in cardiac and neuronal function, impedance is a relatively unexploited technology. The combination of EFP, impedance and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has important implications for safety pharmacology as functional information about contraction and field potentials can be gleaned from human cardiomyocytes in a beating monolayer...
September 2016: Journal of Pharmacological and Toxicological Methods
Divya Rajamohan, Spandan Kalra, Minh Duc Hoang, Vinoj George, Andrew Staniforth, Hugh Russell, Xuebin Yang, Chris Denning
Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a high-throughput manner. Typically, CHO and HEK tumorigenic cell lines overexpressing single ion channels are used since they can be harvested as high-density, homogenous, single-cell suspensions. While human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are physiologically more relevant, these cells are fragile, have complex culture requirements, are inherently heterogeneous, and are expensive to produce, which has restricted their use on automated patch clamp (APC) devices...
March 15, 2016: Stem Cells and Development
Liudmila Polonchuk
Patch-clamping is a powerful technique for investigating the ion channel function and regulation. However, its low throughput hampered profiling of large compound series in early drug development. Fortunately, automation has revolutionized the area of experimental electrophysiology over the past decade. Whereas the first automated patch-clamp instruments using the planar patch-clamp technology demonstrated rather a moderate throughput, few second-generation automated platforms recently launched by various companies have significantly increased ability to form a high number of high-resistance seals...
2014: Methods in Molecular Biology
Nadine Becker, Sonja Stoelzle, Sven Göpel, David Guinot, Patrick Mumm, Claudia Haarmann, Daniela Malan, Heribert Bohlen, Eugen Kossolov, Ralf Kettenhofen, Michael George, Niels Fertig, Andrea Brüggemann
INTRODUCTION: Chip-based automated patch clamp systems are widely used in drug development and safety pharmacology, allowing for high quality, high throughput screening at standardized experimental conditions. The merits of automation generally come at the cost of large amounts of cells needed, since cells are not targeted individually, but randomly positioned onto the chip aperture from cells in suspension. While cell usage is of little concern when using standard cell lines such as CHO or HEK cells, it becomes a crucial constraint with cells of limited availability, such as primary or otherwise rare and expensive cells, like induced pluripotent stem (IPS) cell-derived cardiomyocytes or neurons...
July 2013: Journal of Pharmacological and Toxicological Methods
Carol J Milligan, Clemens Möller
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, ion channels are known to be involved in many disease states and as such have become popular therapeutic targets. For many years now manual patch-clamping has been regarded as one of the best approaches for assaying ion channel function, through direct measurement of ion flow across these membrane proteins...
2013: Methods in Molecular Biology
Liyo Kao, Natalia Abuladze, Xuesi M Shao, Kevin McKeegan, Ira Kurtz
The patch clamp technique is widely used for recording the activity of ion channels in single cells and lipid bilayers. Most platforms utilize borosilicate glass configured as a pipette, however more recently planar patch clamp chips have been developed that require less technical expertise. Planar patch clamp chips in systems like the Nanion Port-a-Patch are useful in that they allow more rapid throughput in drug screening studies. This technique also has the ability to perform rapid solution changes from the intracellular side...
July 15, 2012: Journal of Neuroscience Methods
Cecilia Farre, Alison Haythornthwaite, Claudia Haarmann, Sonja Stoelzle, Mohamed Kreir, Michael George, Andrea Brüggemann, Niels Fertig
Ion channel dysfunction is known to underlie several acute and chronic disorders and, therefore, ion channels have gained increased interest as drug targets. During the past decade, ion channel screening platforms have surfaced that enable high throughput drug screening from a more functional perspective. These two factors taken together have further inspired the development of more refined screening platforms, such as the automated patch clamp platforms described in this article. Approximately six years ago, Nanion introduced its entry level device for automated patch clamping - the Port-a-Patch...
January 2009: Combinatorial Chemistry & High Throughput Screening
Cecilia Farre, Sonja Stoelzle, Claudia Haarmann, Michael George, Andrea Brüggemann, Niels Fertig
Efficient high resolution techniques are required for screening efforts and research targeting ion channels. The conventional patch clamp technique, a high resolution but low efficiency technique, has been established for 25 years. Recent advances have opened up new possibilities for automated patch clamping. This new technology meets the need of drug developers for higher throughput and facilitates new experimental approaches in ion channel research. Specifically, Nanion's electrophysiology workstations, the Port-a-Patch and the Patchliner, have been successfully introduced as high-quality automated patch clamp platforms for industry as well as academic users...
April 2007: Expert Opinion on Therapeutic Targets
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