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https://www.readbyqxmd.com/read/28433003/the-plastid-outer-membrane-localized-lptd1-is-important-for-glycerolipid-remodeling-under-phosphate-starvation
#1
Yi-Ching Hsueh, Christian Ehmann, Nadine Flinner, Roman Ladig, Enrico Schleiff
Glycerolipid synthesis in plants is coordinated between plastids and the endoplasmic reticulum (ER). A central step within the glycerolipid synthesis is the transport of phosphatidic acid from ER to chloroplasts. The chloroplast outer envelope protein TGD4 belongs to the LptD family conserved in bacteria and plants and selectively binds and may transports phosphatidic acid. We describe a second LptD-family protein in A. thaliana (atLPTD1; At2g44640) characterized by a barrel domain with an amino acid signature typical for cyanobacterial LptDs...
April 22, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28432985/lif-endometrial-expression-is-impaired-in-women-with-unexplained-infertility-while-lif-r-expression-in-all-infertility-sub-groups
#2
Chrysoula Margioula-Siarkou, Yannis Prapas, Stamatios Petousis, Stefanos Milias, Konstantinos Ravanos, Themistoklis Dagklis, Ioannis Kalogiannidis, George Mavromatidis, Constantinos Haitoglou, Nikolaos Prapas, David Rousso
The main objective of our study was to study LIF and LIF-R endometrial expression during the implantation window in the various sub-groups of infertile women according to infertility cause. A prospective observational case-control study was performed from March 2013 to February 2016. Infertile women consisted of the patients' group (group 2) while fertile women were the control group (group 1). Infertile women were divided according to infertility cause in women with tubal factor (group 2a), poor ovarian reserve (group 2b), endometriosis (group 2c) and unexplained infertility (group 2d)...
April 19, 2017: Cytokine
https://www.readbyqxmd.com/read/28432942/insights-into-the-diversity-of-nod-like-receptors-identification-and-expression-analysis-of-nlrc3-nlrc5-and-nlrx1-in-rainbow-trout
#3
Claudio A Álvarez, Felipe Ramírez-Cepeda, Paula Santana, Elisa Torres, Jimena Cortés, Fanny Guzmán, Paulina Schmitt, Luis Mercado
Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) are efficient soluble intracellular sensors that activate defense mechanisms against pathogens. In teleost fish, the involvement of NLRs in the immune response is not well understood. However, recent work has evidenced the expression of different NLRs in response to some pathogen associated molecular patterns (PAMPs). In the present work, the cDNA sequence encoding three new NOD-like receptors were identified in Oncorhynchus mykiss, namely OmNLRC3, OmNLRC5 and OmNLRX1...
April 19, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28432899/serum-micrornas-related-with-chemoradiotherapy-resistance-in-advanced-stage-cervical-squamous-cell-carcinoma
#4
Ying Han, Mei Liu, Ziyi Wang, Manni Huang, Ningzhi Xu, Lingying Wu
OBJECTIVE: To investigate the serum microRNAs as biomarkers in predicting chemoradiotherapy resistance in advanced-stage cervical squamous cell carcinoma (ACSCC) patients. METHODS: Serum samples were collected from International Federation of Gynecology and Obstetrics (FIGO) stage IIB to IIIB cervical squamous cell carcinoma patients treated with platinum based Concomitant Chemoradiotherapy (CCRT) in our hospital during September 2013 to November 2015. Twenty well-matched samples (10 resistant and 10 sensitive) were chosen to screen the miRNA expression profile using serum samples pooled with microarrays...
April 19, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28432871/mir-24-3p-is-overexpressed-in-hodgkin-lymphoma-and-protects-hodgkin-and-reed-sternberg-cells-from-apoptosis
#5
Ye Yuan, Joost Kluiver, Jasper Koerts, Debora de Jong, Bea Rutgers, Fazlyn Reeny Abdul Razak, Martijn Terpstra, Boudewijn Plaat, Ilja M Nolte, Arjan Diepstra, Lydia Visser, Klaas Kok, Anke van den Berg
miRNAs play important roles in biological processes, such as proliferation, metabolism, differentiation, and apoptosis, whereas altered expression levels contribute to diseases, such as cancers. We identified miRNAs with aberrant expression in Hodgkin lymphoma (HL) and investigated their role in pathogenesis. Small RNA sequencing revealed 84 significantly differentially expressed miRNAs in HL cell lines as compared to germinal center B cells. Three up-regulated miRNAs-miR-23a-3p, miR-24-3p, and miR-27a-3p-were derived from one primary miRNA transcript...
April 19, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28432852/correlation-between-expression-of-high-temperature-requirement-serine-protease-a1-htra1-in-nucleus-pulposus-and-t2-value-of-magnetic-resonance-imaging
#6
Dapeng Li, Jiawei Yue, Lu Jiang, Yonghui Huang, Jifu Sun, Yan Wu
BACKGROUND Degrading enzymes play an important role in the process of disc degeneration. The objective of this study was to investigate the correlation between the expression of high temperature requirement serine protease A1 (HtrA1) in the nucleus pulposus and the T2 value of the nucleus pulposus region in magnetic resonance imaging (MRI). MATERIAL AND METHODS Thirty-six patients who had undergone surgical excision of the nucleus pulposus were examined by MRI before surgery. Pfirrmann grading of the target intervertebral disc was performed according to the sagittal T2-weighted imaging, and the T2 value of the target nucleus pulposus was measured according to the median sagittal T2 mapping...
April 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28432832/profiling-microrna-from-nephrectomy-and-biopsy-specimens-predictors-of-progression-and-survival-in-clear-cell-renal-cell-carcinoma
#7
Casey G Kowalik, Drew A Palmer, Travis B Sullivan, Patrick A Teebagy, John M Dugan, John A Libertino, Eric J Burks, David Canes, Kimberly M Rieger-Christ
OBJECTIVE: To identify miRNA characteristic of metastatic clear cell renal cell carcinoma (ccRCC) and those indicative of cancer specific survival in nephrectomy and biopsy specimens. We also sought to determine if a miRNA panel could differentiate benign from ccRCC tissue. MATERIALS AND METHODS: RNA was isolated from nephrectomy and kidney biopsy specimens (n=156; n=46 respectively). Samples were grouped: benign, non-progressive and progressive ccRCC. MiRNA were profiled by microarray and validated by qRT-PCR...
April 22, 2017: BJU International
https://www.readbyqxmd.com/read/28432817/factors-involved-in-early-polarization-of-the-anterior-posterior-axis-in-the-milkweed-bug-oncopeltus-fasciatus
#8
Neta Ginzburg, Mira Cohen, Ariel D Chipman
The axes of insect embryos are defined early in the blastoderm stage. Genes involved in this polarization are well known in Drosophila, but less so in other insects, such as the milkweed bug Oncopeltus fasciatus. Using quantitative PCR, we looked at differential expression of several candidate genes for early anterior-posterior patterning and found that none of them are expressed asymmetrically in the early blastoderm. We then used an RNA-Seq approach to identify novel candidate genes that might be involved in early polarization in Oncopeltus...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432787/presynaptic-morphology-and-vesicular-composition-determine-vesicle-dynamics-in-mouse-central-synapses
#9
Laurent Guillaud, Dimitar Dimitrov, Tomoyuki Takahashi
Transport of synaptic vesicles (SVs) in nerve terminals is thought to play essential roles in maintenance of neurotransmission. To identify factors modulating SV movements, we performed real-time imaging analysis of fluorescently labeled SVs in giant calyceal and conventional hippocampal terminals. Compared with small hippocampal terminals, SV movements in giant calyceal terminals were faster, longer and kinetically more heterogeneous. Morphological maturation of giant calyceal terminals was associated with an overall reduction in SV mobility and displacement heterogeneity...
April 22, 2017: ELife
https://www.readbyqxmd.com/read/28432783/gene-structure-and-expression-characteristic-of-a-novel-odorant-receptor-gene-cluster-in-the-parasitoid-wasp-microplitis-mediator-hymenoptera-braconidae
#10
S-N Wang, S Shan, Y Zheng, Y Peng, Z-Y Lu, Y-Q Yang, R-J Li, Y-J Zhang, Y-Y Guo
Odorant receptors (ORs) expressed in the antennae of parasitoid wasps are responsible for detection of various lipophilic airborne molecules. In the present study, 107 novel OR genes were identified from Microplitis mediator antennal transcriptome data. Phylogenetic analysis of the set of OR genes from M. mediator and Microplitis demolitor revealed that M. mediator OR (MmedOR) genes can be classified into different subfamilies, and the majority of MmedORs in each subfamily shared high sequence identities and clear orthologous relationships to M...
April 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28432719/effect-of-ficus-carica-leaf-extract-on-the-gene-expression-of-selected-factors-in-hacat-cells
#11
Murat Turkoglu, Erkin Pekmezci, Songul Kilic, Cihat Dundar, Hakan Sevinc
BACKGROUND: Ficus carica Linn. (Fc), common fig, has been traditionally used for many metabolic, cardiovasculary, respiratory, gastrointestinal, and skin disorders. Several studies were performed showing its anti-inflammatory, anti-angiogenic, anticancerogenic, and tissue-protective effects. In all of those studies, the positive effects of Fc were concluded as the result of its antioxidant and anti-inflammatory features due to the polyphenols it contains. AIMS: To study the phenolic compounds of Fc extract and to investigate the molecular basis of anti-inflammatory, anti-angiogenic, antimitotic, and anti-androgenic effects of Fc leaf extract in vitro...
April 22, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28432698/quantitative-evaluation-of-metal-artifacts-using-different-cbct-devices-high-density-materials-and-field-of-views
#12
Marina Codari, Karla de Faria Vasconcelos, Laura Ferreira Pinheiro Nicolielo, Francisco Haiter Neto, Reinhilde Jacobs
OBJECTIVE: To objectively compare the influence of different cone-beam computed tomography (CBCT) devices, high-density materials and field of views (FOVs) on metal artifact expression. MATERIAL AND METHODS: For this in vitro study, three customized acrylic resin phantoms containing high-density materials cylinders: titanium, copper-aluminum alloy and amalgam were scanned on three CBCT devices using high-resolution protocols, same voxel size (0.2 mm) and different FOVs...
April 22, 2017: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/28432628/design-of-water-quality-monitoring-networks-with-two-information-scenarios-in-tropical-andean-basins
#13
Juan Carlos Bastidas, Jorge Julián Vélez, Jeannette Zambrano, Adela Londoño
Design and redesign of water quality monitoring networks were evaluated for two similarly sized watersheds in the tropical Andes via optimization techniques using geographic information system technology (GIS) and a matter-element analysis of 5-day biological oxygen demand (BOD5) and total suspended solids (TSS). This resulted in a flexible, objectively based design for a 1128-km(2) watershed without prior water quality data (La Miel River), and a network redesign of a 1052-km(2) watershed with historical water quality monitoring (Chinchiná River)...
April 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28432622/diversity-shift-in-bacterial-phenol-hydroxylases-driven-by-alkyl-phenols-in-oil-refinery-wastewaters
#14
Besma Harzallah, Hacène Bousseboua, Yves Jouanneau
Phenol hydroxylases (PHs) play a primary role in the bacterial degradation of phenol and alkylphenols. They are divided into two main classes, single-component and multi-component PHs, having distinctive catalytic subunits designated as PheA1 and LmPH, respectively. The diversity of these enzymes is still largely unexplored. Here, both LmPH and pheA1 gene sequences were examined in activated sludge from oil refinery wastewaters. Phenol, p-cresol, or 3,4-dimethylphenol (3,4-DMP) supplied as extra carbon sources were rapidly mineralized by the microbial community...
April 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28432618/the-identification-of-key-genes-and-pathways-in-hepatocellular-carcinoma-by-bioinformatics-analysis-of-high-throughput-data
#15
Chaoyang Zhang, Li Peng, Yaqin Zhang, Zhaoyang Liu, Wenling Li, Shilian Chen, Guancheng Li
Liver cancer is a serious threat to public health and has fairly complicated pathogenesis. Therefore, the identification of key genes and pathways is of much importance for clarifying molecular mechanism of hepatocellular carcinoma (HCC) initiation and progression. HCC-associated gene expression dataset was downloaded from Gene Expression Omnibus database. Statistical software R was used for significance analysis of differentially expressed genes (DEGs) between liver cancer samples and normal samples. Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, based on R software, were applied for the identification of pathways in which DEGs significantly enriched...
June 2017: Medical Oncology
https://www.readbyqxmd.com/read/28432590/pathological-analysis-of-the-surgical-margins-of-resected-glioblastomas-excised-using-photodynamic-visualization-with-both-5-aminolevulinic-acid-and-fluorescein-sodium
#16
Hirohito Yano, Noriyuki Nakayama, Naoyuki Ohe, Kazuhiro Miwa, Jun Shinoda, Toru Iwama
During glioma resection, 5-aminolevulinic acid (5-ALA) and fluorescein sodium (Fl-Na) are used for photodynamic tumor visualization. The objective of this study was to evaluate the pathological findings of the boundary zone between the tumor and adjacent normal brain in glioblastoma patients undergoing simultaneous double staining with 5-ALA and Fl-Na during surgery. Eight patients received 5-ALA (20 mg/kg orally) before the induction of general anesthesia, and Fl-Na (20 mg/kg) was administered intravenously before the dural incision was performed...
April 21, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28432586/cdc20-with-malignant-progression-and-poor-prognosis-of-astrocytoma-revealed-by-analysis-on-gene-expression
#17
Yiming Ding, Shuqing Yu, Zhaoshi Bao, Yanwei Liu, Tingyu Liang
The malignant transformation of astrocytoma may result from the accumulation of multiple genetic alterations. Current research shows that diffuse astrocytoma (AIIs, WHO grade II) is inherently predisposed to recur locally, and to spontaneously progress to anaplastic astrocytoma (AAIIIs, WHO grade III) and eventually secondary glioblastoma (sGBMIVs, WHO grade IV). The aim of the study was to identify and validate the important gene(s) associated with malignant progression and poor prognosis of astrocytoma. Average expression levels of 82 samples (35 AIIs, 13 AAIIIs and 34 sGBMIVs) were compared to each other through no-paired student test...
April 21, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28432535/exploring-the-carbamazepine-interaction-with-human-pregnane-x-receptor-and-effect-on-abcc2-using-in-vitro-and-in-silico-approach
#18
Gurpreet K Grewal, Khuraijam D Singh, Neha Kanojia, Chitra Rawat, Samiksha Kukal, Ajay Jajodia, Anshika Singhal, Richa Misra, Selvaraman Nagamani, Karthikeyan Muthusamy, Ritushree Kukreti
PURPOSE: Over expression of ATP-binding cassette transporters is considered one of the major reasons for non-responsiveness to antiepileptic drugs. Carbamazepine (CBZ), one of first line antiepileptic drug is known to influence ABCC2 expression but its exact molecular mechanism is unknown. METHODS: We investigated the effect of CBZ on expression of ABCC2 and pregnane X receptor (PXR) in HepG2 cell line and compared with hyperforin (agonist of PXR) and ketoconazole (antagonist of PXR) through realtime PCR and western blot assay...
April 21, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28432512/squamous-cell-carcinoma-of-the-breast-in-the-united-states-incidence-demographics-tumor-characteristics-and-survival
#19
Siddhartha Yadav, Dhiraj Yadav, Dana Zakalik
PURPOSE: Squamous cell carcinoma of breast accounts for less than 0.1% of all breast cancers. The purpose of this study is to describe the epidemiology and survival of this rare malignancy. METHODS: Data were extracted from the National Cancer Institute's Surveillance, Epidemiology and End Results Registry to identify women diagnosed with squamous cell carcinoma of breast between 1998 and 2013. SEER*Stat 8.3.1 was used to calculate age-adjusted incidence, age-wise distribution, and annual percentage change in incidence...
April 21, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28432433/glypican-3-mrna-expression-level-in-wilms-tumor-correlation-with-histological-type-stage-and-outcome
#20
Md Nahidul Wari, Archana George Vallonthaiel, Aijaz Ahmed, Deepali Saxena, Venkateswaran K Iyer, Sandeep R Mathur, Sandeep Agarwala, Sameer Bakhshi, V Srinivas, P Chattopadhyaya, Arundhati Sharma, S Datta Gupta, Amit Dinda
PURPOSE: To correlate expression of Glypican-3 in Wilms tumor with histopathology, stage, and outcome. METHODS: Glypican-3 mRNA expression by real-time PCR on tumor and normal germline samples from 75 fresh nephrectomies for Wilms tumor with fold change after normalization against GAPDH was compared. Survival analysis for event-free and overall survival (EFS, OS) with 2-year follow-up for Glypican-3 overexpression (>1.5 times) and clinicopathological parameters was performed...
April 21, 2017: Pediatric Surgery International
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