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https://www.readbyqxmd.com/read/29656620/the-effect-of-recombinant-tyrosine-hydroxylase-expression-on-the-neurogenic-differentiation-potency-of-mesenchymal-stem-cells
#1
Gizem Turac, Gokhan Duruksu, Erdal Karaoz
OBJECTIVE: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in dopamine synthesis, making the enhancement of its activity a target for ensuring sufficient dopamine levels. Rat bone marrow mesenchymal stem cells (rBM-MSCs) are known to synthesize TH after differentiating into neuronal cells through chemical induction, but the effect of its ectopic expression on these cells has not yet been determined. This study investigated the effects of ectopic recombinant TH expression on the stemness characteristics of rBM-MSCs...
March 2018: Neurospine
https://www.readbyqxmd.com/read/29656577/efficacy-of-7-benzyloxyindole-and-other-halogenated-indoles-to-inhibit-candida-albicans-biofilm-and-hyphal-formation
#2
Ranjith Kumar Manoharan, Jin-Hyung Lee, Jintae Lee
Certain pathogenic bacteria and yeast form biofilms on biotic and abiotic surfaces including medical devices and implants. Hence, the development of antibiofilm coating materials becomes relevant. The virulence of those colonizing pathogens can be reduced by inhibiting biofilm formation rather than killing pathogens using excessive amounts of antimicrobials, which is touted as one of the main reasons for the development of drug resistance. Candida albicans is an opportunistic fungal pathogen, and the transition of yeast cells to hyphal cells is believed to be a crucial virulence factor...
April 15, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29656515/comparative-cyp-omic-analysis-between-the-ddt-susceptible-and-resistant-drosophila-melanogaster-strains-91-c-and-91-r
#3
Keon Mook Seong, Brad S Coates, May R Berenbaum, John M Clark, Barry R Pittendrigh
BACKGROUND: Cytochrome P450 monooxygenases (P450s) are involved in the biosynthesis of endogenous intracellular compounds and the metabolism of xenobiotics, including chemical insecticides. We investigated the structural and expression level variance across all P450 genes with respect to the evolution of insecticide resistance under multigenerational DDT selection. RESULTS: RNA-seq and reverse transcriptase quantitative PCR indicated that the transcript levels of seven P450 genes were significantly up-regulated and three P450 genes were down-regulated in the DDT-resistant strain 91-R, as compared to the control strain 91-C...
April 15, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29656499/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-analysis-of-36-blood-group-alleles-among-396-thai-samples-reveals-region-specific-variants
#4
Philaiphon Jongruamklang, Christoph Gassner, Stefan Meyer, Aksarakorn Kummasook, Marion Darlison, Chayanun Boonlum, Surin Chanta, Beat M Frey, Martin L Olsson, Jill R Storry
BACKGROUND: Blood group phenotype variation has been attributed to potential resistance to pathogen invasion. Variation was mapped in blood donors from Lampang (northern region) and Saraburi (central region), Thailand, where malaria is endemic. The previously unknown blood group allele profiles were characterized and the data were correlated with phenotypes. The high incidence of the Vel-negative phenotype previously reported in Thais was investigated. STUDY DESIGN AND METHODS: DNA from 396 blood donors was analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry...
April 15, 2018: Transfusion
https://www.readbyqxmd.com/read/29656462/kdm1a-regulated-the-osteo-dentinogenic-differentiation-process-of-the-stem-cells-of-the-apical-papilla-via-binding-with-plod2
#5
Lijun Wang, Haoqing Yang, Xiao Lin, Yangyang Cao, Peipei Gao, Ying Zheng, Zhipeng Fan
OBJECTIVES: Dental tissue-derived mesenchymal stem cells (MSCs)-mediated pulp-dentin regeneration is considered a potential approach for the regeneration of damaged teeth. Enhancing MSC-mediated pulp-dentin regeneration is based on an understanding of the molecular mechanisms underlying directed cell differentiation process. Histone demethylation enzyme, lysine demethylase 1A (KDM1A) can regulate the differentiation of some MSCs, but its role in dental tissue-derived MSCs is unclear. MATERIAL AND METHODS: We obtained SCAPs from immature teeth...
April 15, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29656436/comparative-analysis-of-the-toxicity-of-gold-nanoparticles-in-zebrafish
#6
Srinath Patibandla, Yinan Zhang, Ali Mohammad Tohari, Peng Gu, James Reilly, Yu Chen, Xinhua Shu
The use of nanoparticles - particles that range in size from 1 to 100 nm - has become increasingly prevalent in recent years, bringing with it a variety of potential toxic effects. Zebrafish embryos were exposed during the 3 day postfertilization period to gold nanospheres (GNSs), gold nanorods (GNRs), GNRs coated with polystyrene sulphate (PSS-GNRs) and GNRs coated with both PSS and polyallamine hydrochloride (PAH-PSS-GNRs). All nanorods were stabilized with cetyltrimethylammonium bromide. GNSs were the least toxic of the nanoparticles studied, with exposure resulting in no significant changes in mortality, hatching or heart rate...
April 15, 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29656435/a-multi-omic-study-reveals-btg2-as-a-reliable-prognostic-marker-for-early-stage-non-small-cell-lung-cancer
#7
Sipeng Shen, Ruyang Zhang, Yichen Guo, Elizabeth Loehrer, Yongyue Wei, Ying Zhu, Qianyu Yuan, Sebastian Moran, Thomas Fleischer, Maria Moksnes Bjaanaes, Anna Karlsson, Maria Planck, Johan Staaf, Åslaug Helland, Manel Esteller, Li Su, Feng Chen, David C Christiani
B-cell translocation gene 2 (BTG2) is a tumor suppressor protein and is known to be downregulated in several types of cancer. In this study, we investigated a potential role for BTG2 in early-stage non-small cell lung cancer (NSCLC) survival. We analysed BTG2 methylation data from 1,230 early-stage NSCLC patients from five international cohorts, as well as gene expression data from 3,038 lung cancer cases from multiple cohorts. Three CpG probes (cg01798157, cg06373167, cg23371584) that detected BTG2 hypermethylation in tumor tissues were associated with lower overall survival...
April 15, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29656391/proteomic-analysis-of-sperm-proteins-in-infertile-men-with-high-levels-of-reactive-oxygen-species
#8
A Ayaz, A Agarwal, R Sharma, N Kothandaraman, Z Cakar, S Sikka
Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality. Patients and donors were divided into ROS (-) and ROS (+) groups. Using computational studies, and data mining of available literature on spermatozoa, oxidative stress and proteomics, we identified three core regulatory proteins angiotensin-converting enzyme (ACE), heat-shock protein (Hsp70) family A member 2 (HSPA2) and ribosomal protein subunit 27A (RPS27A) and seven interlink proteins NOS2, SUMO2, UBL4A, FBXO25, MAP3K3, APP and UBC...
April 15, 2018: Andrologia
https://www.readbyqxmd.com/read/29656342/prosaposin-and-its-receptors-are-differentially-expressed-in-the-salivary-glands-of-male-and-female-rats
#9
Farzana Islam, Md Sakirul Islam Khan, Hiroaki Nabeka, Shouichiro Saito, Xuan Li, Tetsuya Shimokawa, Kimiko Yamamiya, Naoto Kobayashi, Seiji Matsuda
Salivary glands produce various neurotrophins that are thought to regulate salivary function during normal and pathological conditions. Prosaposin (PSAP) is a potent neurotrophin found in several tissues and various biological fluids and may play roles in the regulation of salivary function. However, little is known about PSAP in salivary glands. As the functions of salivary glands are diverse based on age and sex, this study examines whether PSAP and its receptors, G protein-coupled receptor 37 (GPR37) and GPR37L1, are expressed in the salivary glands of rats and whether sex and aging affect their expression...
April 14, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29656327/analysis-of-regeneration-and-myelination-associated-proteins-in-human-neuroma-in-continuity-and-discontinuity
#10
Patrick Dömer, Bettina Kewitz, Christian P G Heinen, Ulrike Janssen-Bienhold, Thomas Kretschmer
BACKGROUND: Neuromas are pathologic nerve distensions caused by a nerve's response to trauma, resulting in a dysfunctional to non-functional nerve. Depending on the severance of the affected nerve, the resulting neuroma can be differentiated into continuous and stump neuroma. While neuroma formation has been investigated in animal models with enormous regenerative capacity, the search for differences in human response to nerve trauma on a molecular level ultimately seeks to identify reasons for functionally successful versus unsuccessful regeneration after peripheral nerve trauma in man...
April 14, 2018: Acta Neurochirurgica
https://www.readbyqxmd.com/read/29656307/alveolar-bone-protective-effect-of-hiziki-extracts-on-the-progression-of-periodontitis
#11
Don-Gil Lee, Yu-Kyong Shin, Jae-Hee Park, Sang-Yong Park, Eunson Hwang, Jung-Eun Yang, Hae Jo, Ki-Young Kim, Gafurjon T Mavlonov, Tae-Hoo Yi
The purpose of this study was to evaluate the effects of hiziki extract on alveolar bone loss, inflammation, and osteo-biomarker expression in hPDL cells (10, 50, 100 μg/ml final concentrations in culture medium) and on a ligature-induced periodontitis rat model (50, 100, 200 mg/kg with oral administration). Hiziki extract increased alkaline phosphatase activity and mineralized nodule formation in hPDL cell. In western blot analysis, hiziki extract resulted in increased expression of osteoblast markers, including transforming growth factor beta (TGF-β), SMAD anchor for receptor activation (SARA) and runt-related transcription factor 2 (RUNX2) in hDPL cells...
April 14, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29656289/high-concentration-of-glial-cell-line-derived-neurotrophic-factor-protects-primary-astrocytes-from-apoptosis
#12
Yuqian Wang, Yuxia Qin, Tingwen Guo, Chuanxi Tang, Liyun Liu, Dianshuai Gao
BACKGROUND: Studies have shown that astrocytes play an important role in a variety of biological processes, so damage to astrocytes can cause a series of related diseases. Glial cell line-derived neurotrophic factor (GDNF) has always been considered a protective factor for dopamine neurons. However, it remains unclear whether GDNF has a protective effect on glial cells, especially astrocytes. In this study, we put forward the hypothesis that a high concentration of GDNF in the microenvironment of astrocytes exerts an inhibitory effect on the apoptosis of astrocytes by DNA-damaging reagents...
April 13, 2018: Developmental Neuroscience
https://www.readbyqxmd.com/read/29656222/prolactin-induced-protein-pip-is-a-potential-biomarker-for-early-stage-and-malignant-breast-cancer
#13
Anju Gangadharan, Themba Nyirenda, Kishan Patel, Nydia Jaimes-Delgadillo, Dominique Coletta, Takemi Tanaka, Ayal C Walland, Zena Jameel, Srinivasa Vedantam, Sittinon Tang, Ciaran Mannion, Grace Y Lee, Andre Goy, Andrew Pecora, K Stephen Suh
OBJECTIVES: Breast cancer (BC) is the second leading cause of cancer-related mortality in women. Bioinformatic analysis and expression screening showed that Prolactin Induced Protein (PIP) was differentially expressed in BC. The objective of this investigation was to characterize the expression pattern of PIP, an aspartyl proteinase, in malignant and non-malignant breast tissues. MATERIALS AND METHODS: Real time quantitative PCR was employed to analyze PIP and androgen receptor (AR) mRNA levels in BC cell lines and 190 normal tissues and tumor samples...
April 12, 2018: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/29656109/bile-acids-are-important-direct-and-indirect-regulators-of-the-secretion-of-appetite-and-metabolism-regulating-hormones-from-the-gut-and-pancreas
#14
Rune E Kuhre, Nicolai J Wewer Albrechtsen, Olav Larsen, Sara L Jepsen, Emilie Balk-Møller, Daniel B Andersen, Carolyn F Deacon, Kristina Schoonjans, Frank Reimann, Fiona M Gribble, Reidar Albrechtsen, Bolette Hartmann, Mette M Rosenkilde, Jens J Holst
OBJECTIVE: Bile acids (BAs) facilitate fat absorption and may play a role in glucose and metabolism regulation, stimulating the secretion of gut hormones. The relative importance and mechanisms involved in BA-stimulated secretion of appetite and metabolism regulating hormones from the gut and pancreas is not well described and was the purpose of this study. METHODS: The effects of bile acids on the secretion of gut and pancreatic hormones was studied in rats and compared to the most well described nutritional secretagogue: glucose...
March 17, 2018: Molecular Metabolism
https://www.readbyqxmd.com/read/29656020/odorant-binding-protein-based-identification-of-natural-spatial-repellents-for-the-african-malaria-mosquito-anopheles-gambiae
#15
Thomas Kröber, Konstantinos Koussis, Martine Bourquin, Panagiota Tsitoura, Maria Konstantopoulou, Taiwo Sam Awolola, Francesca R Dani, Huili Qiao, Paolo Pelosi, Kostas Iatrou, Patrick M Guerin
There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, with a predominantly female expression bias to identify plant essential oils (EOs) containing bioactive compounds that target mosquito olfactory function. EOs and their fractions capable of binding to such OBPs displayed repellence against female mosquitoes in a laboratory repellence assay...
April 12, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29655989/in-vivo-migration-of-mesenchymal-stem-cells-to-burn-injury-sites-and-their-therapeutic-effects-in-a-living-mouse-model
#16
Eun Jung Oh, Ho Won Lee, Senthilkumar Kalimuthu, Tae Jung Kim, Hyun Mi Kim, Se Hwan Baek, Liya Zhu, Ji Min Oh, Seung Hyun Son, Ho Yun Chung, Byeong-Cheol Ahn
Mesenchymal stem cell (MSC)-based therapy has emerged as a promising therapeutic strategy for tissue regeneration and repair. In this study, we non-invasively monitored the tracking of MSCs toward burn injury sites using MSCs expressing firefly luciferase (Fluc) gene in living mice, and evaluated the effects of the MSCs at the injury site. Murine MSCs co-expressing Fluc and green fluorescent protein (GFP) were established using a retroviral system (referred to as MSC/Fluc). To evaluate the ability of MSC migration toward burn injury sites, cutaneous burn injury was induced in the dorsal skin of mice...
April 12, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29655919/endocannabinoid-system-in-systemic-lupus-erythematosus-first-evidence-for-a-deranged-2-arachidonoylglycerol-metabolism
#17
Luca Navarini, Tiziana Bisogno, Pamela Mozetic, Fabiana Piscitelli, Domenico Paolo Emanuele Margiotta, Fabio Basta, Antonella Afeltra, Mauro Maccarrone
The endocannabinoid (eCB) system plays a key role in many physiological and pathological conditions and its dysregulation has been described in several rheumatological and autoimmune diseases. Yet, its possible alteration in systemic lupus erythematosus (SLE) has never been investigated. Here, we aimed filling this gap in plasma and peripheral blood mononuclear cells (PBMCs) of patients with SLE and age- and sex- matched healthy subjects (HS). Liquid chromatography-mass spectrometry quantitation of eCB levels highlighted that plasma levels of 2-arachidonoylglycerol (2-AG) were significantly increased in SLE patients compared to HS (p = 0...
April 12, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29655896/differential-expression-profile-of-long-non-coding-rna-in-the-stenosis-tissue-of-arteriovenous-fistula
#18
Yan Yan, Wen Ye, Qinkai Chen, Liu Yang, Li Zhang, Yu Liu, Xiaochen Zhou, Gongxian Wang
OBJECTIVE: To analyze and preliminarily validate the lncRNA expression profiles in the stenosis tissue of arteriovenous fistula (AVF). METHODS: A total of 54 uremic patients administered in the department of nephrology in the First Affiliated Hospital of Nanchang University between February 2017 and March 2017 were included in the study and subsequently categorized as experimental group, which included 12 patients with confirmed diagnosis of AVF, and control group which included 42 patients with no vascular stenosis...
April 12, 2018: Gene
https://www.readbyqxmd.com/read/29655887/engineering-riboswitch-in-l-major-from-prediction-to-conceptualization
#19
Pruthvi Raj Bejugam, Shailza Singh
Post-transcriptional gene regulation is a vital process to regulate expression of the key genes in the eukaryotic cell. Such processes are essential for pathogens which reside inside the host cell. One such pathogen is Leishmania major, which causes cutaneous leishmaniasis. The parasite lives inside the macrophages of mammalian host (mostly human). Inside the macrophage, Leishmania genes show complex host-pathogen interaction regulating a plethora of gene expression. Till date, most of the studies have shown this kind of regulation with respect to the host macrophages...
April 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29655871/cloning-partial-sequencing-and-expression-analysis-of-the-neural-form-of-p450-aromatase-cyp19a1b-in-the-south-america-catfish-rhamdia-quelen
#20
Helena C Silva de Assis, Laia Navarro-Martín, Letícia S P Fernandes, Cibele C Cardoso, Daniela Parada Pavoni, Vance L Trudeau
Brain aromatase is a key enzyme exclusively expressed in fish radial glial cells that convert androgens into estrogens, thus controlling neuroendocrine functions and neurogenesis. As an important step in characterizing the neuroendocrine systems of Rhamdia quelen (jundiá), a partial cDNA sequence (1045 bp) of brain aromatase (cyp19a1b) was cloned and sequenced. At the nucleotide level the cDNA sequence was found to be 88% identical to cyp19a1b of two species of catfish, Ictalurus punctatus and Silurus meridionalis...
April 12, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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