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https://www.readbyqxmd.com/read/27923262/circulating-levels-of-lamp2-in-coronary-artery-disease-association-with-serum-lipid-profile
#1
M Teimouri, P Shabani, F Zali, M Najafi, H Shateri, M Asadnia, A Vatannejad, M Najafi, M Doosti
Whereas several in vitro and in vivo studies have described the role of lysosomal associated membrane protein 2 (LAMP2) in lipid homeostasis, there is no study addressing LAMP2 serum concentration and its association with lipid profiles in the context of coronary artery disease (CAD). We aimed to determine the LAMP2 serum concentration and its association with serum lipid profiles as well as the gene expression of LAMP2 in peripheral blood mononuclear cells (PBMCs) of CAD patients and control group. Circulating levels of LAMP2 were quantified by enzyme-linked immunosorbent assay (ELISA) in CAD patients (n=85) and control group (n=65) and correlation to lipid parameters was assessed...
December 6, 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/27923188/electrophysiology-in-fisher-syndrome
#2
REVIEW
Satoshi Kuwabara, Yukari Sekiguchi, Sonoko Misawa
Fisher syndrome (FS), a variant of Guillain-Barré syndrome (GBS), is characterized by the clinical triad of ophthalmoplegia, ataxia, and areflexia. The lesion sites for these unique clinical features include the oculomotor nerves and group 1a neurons in the dorsal root ganglion, and the presence of FS is determined by the expression of ganglioside GQ1b in the human nervous system. Neurophysiological findings suggest that ataxia and areflexia are due to an impaired proprioceptive afferent system. Typically, the soleus H-reflex is absent and a body-sway analysis using posturography shows a 1-Hz peak, which indicates proprioception dysfunction...
November 20, 2016: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/27923109/generation-of-domain-specific-monoclonal-antibodies-against-human-glutaredoxin3
#3
Xin Dai, Yanqing Li, Xiaohong Sun, Kai Cai, Qinwen Mao, Haibin Xia
Human Glutaredoxin3 (hGLRX3), which encodes a 37.4 kDa protein, possesses an N-terminal Trx homology domain followed by two tandem repeats of Grx domains. GLRX3 is expressed in many tissues and plays important roles in iron metabolism, antioxidant effect, cell proliferation and development, regulation of immune reaction, and tumorigenesis. The mechanisms underlying the biological function of GLRX3 are still not clear. To facilitate the functional research of GLRX3, in this study, monoclonal antibodies (MAbs) against hGLRX3 were produced by using purified prokaryotic recombinant 6His-hGLRX3 fusion protein as the immunogen...
December 6, 2016: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/27923070/macrophage-colony-stimulating-factor-derived-from-cd4-t-cells-contributes-to-control-of-a-blood-borne-infection
#4
Mary F Fontana, Gabrielly L de Melo, Chioma Anidi, Rebecca Hamburger, Chris Y Kim, So Youn Lee, Jennifer Pham, Charles C Kim
Dynamic regulation of leukocyte population size and activation state is crucial for an effective immune response. In malaria, Plasmodium parasites elicit robust host expansion of macrophages and monocytes, but the underlying mechanisms remain unclear. Here we show that myeloid expansion during P. chabaudi infection is dependent upon both CD4+ T cells and the cytokine Macrophage Colony Stimulating Factor (MCSF). Single-cell RNA-Seq analysis on antigen-experienced T cells revealed robust expression of Csf1, the gene encoding MCSF, in a sub-population of CD4+ T cells with distinct transcriptional and surface phenotypes...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27923049/expression-of-lncrnas-in-low-grade-gliomas-and-glioblastoma-multiforme-an-in-silico-analysis
#5
Brian J Reon, Jordan Anaya, Ying Zhang, James Mandell, Benjamin Purow, Roger Abounader, Anindya Dutta
BACKGROUND: Each year, over 16,000 patients die from malignant brain cancer in the US. Long noncoding RNAs (lncRNAs) have recently been shown to play critical roles in regulating neurogenesis and brain tumor progression. To better understand the role of lncRNAs in brain cancer, we performed a global analysis to identify and characterize all annotated and novel lncRNAs in both grade II and III gliomas as well as grade IV glioblastomas (glioblastoma multiforme [GBM]). METHODS AND FINDINGS: We determined the expression of all lncRNAs in over 650 brain cancer and 70 normal brain tissue RNA sequencing datasets from The Cancer Genome Atlas (TCGA) and other publicly available datasets...
December 2016: PLoS Medicine
https://www.readbyqxmd.com/read/27923045/tumor-evolution-in-two-patients-with-basal-like-breast-cancer-a-retrospective-genomics-study-of-multiple-metastases
#6
Katherine A Hoadley, Marni B Siegel, Krishna L Kanchi, Christopher A Miller, Li Ding, Wei Zhao, Xiaping He, Joel S Parker, Michael C Wendl, Robert S Fulton, Ryan T Demeter, Richard K Wilson, Lisa A Carey, Charles M Perou, Elaine R Mardis
BACKGROUND: Metastasis is the main cause of cancer patient deaths and remains a poorly characterized process. It is still unclear when in tumor progression the ability to metastasize arises and whether this ability is inherent to the primary tumor or is acquired well after primary tumor formation. Next-generation sequencing and analytical methods to define clonal heterogeneity provide a means for identifying genetic events and the temporal relationships between these events in the primary and metastatic tumors within an individual...
December 2016: PLoS Medicine
https://www.readbyqxmd.com/read/27923017/expression-of-microrna-328-functions-as-a-biomarker-for-recurrence-of-early-gastric-cancer-egc-after-endoscopic-submucosal-dissection-esd-by-modulating-cd44
#7
Hui-Guang Xue, Ai-Hua Yang, Xue-Guo Sun, Yan-Yan Lu, Zi-Bin Tian
BACKGROUND This study investigated the molecular mechanism of the effect of CD44 on the recurrence of EGC after ESD, including the potential regulator and signaling pathways of CD44. MATERIAL AND METHODS We searched the miRNA online database (www.mirdb.org) with the "seed sequence" located within the 3'-UTR of the target gene, and performed luciferase assay to test the miRNA/mRNA relationship. We also determined the expression of CD44 in the EGC and control samples. In addition, statistical analysis was used to explore the role of miR-328 as a biomarker to predict the recurrence after ECD...
December 6, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27922982/mir-21-is-overexpressed-in-hydatidiform-mole-tissues-and-promotes-proliferation-migration-and-invasion-in-choriocarcinoma-cells
#8
Ya-Xin Wang, Jiu-Ru Zhao, Yue-Ying Xu, Wei-Bin Wu, Hui-Juan Zhang
OBJECTIVE: The aims of this study were to make clear whether miR-21 was dysregulated in hydatidiform mole (HM) tissues and choriocarcinoma (CCA) cells, to elucidate whether aberrant miR-21 expression would affect the function of CCA cells, and to find out whether there was a relationship between miR-21 and AKT, PDCD4, and PTEN in CCA cells. METHODS: Fresh and formalin-fixed, paraffin-embedded trophoblastic tissues (normal first trimester placentas and HMs) were retrieved from the biobank in the International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University...
December 3, 2016: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27922941/exercise-training-ameliorates-glucosamine-induced-insulin-resistance-in-ovariectomized-rats
#9
Chung-Hwan Chen, Tsang-Hai Huang, Tsung-Lin Cheng, Chi-Fen Chang, Chau-Zen Wang, Meng-Hsing Wu, Lin Kang
OBJECTIVE: Glucosamine (GlcN), which has been reported to induce insulin resistance (IR), is a popular nutritional supplement used to treat osteoarthritis in menopausal women. We previously demonstrated that GlcN treatment caused IR in ovariectomized rats by reducing the expression of glucose transport protein subtype 4 (GLUT-4) in skeletal muscle. In the present study, we hypothesized that endurance exercise training can reverse GlcN-induced IR. METHODS: Fifty female rats were randomly divided into five groups with 10 rats in each group: (1) sham-operated group; (2) sham-operated group with GlcN treatment for 14 days; (3) ovariectomy (OVX) group; (4) OVX with GlcN treatment; and (5) OVX with GlcN treatment followed by exercise training (running program) for 8 weeks...
December 5, 2016: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/27922938/skeletal-muscle-mass-is-associated-with-higher-dietary-protein-intake-and-lower-body-fat-in-postmenopausal-women-a-cross-sectional-study
#10
Thaís R Silva, Poli M Spritzer
OBJECTIVE: We investigated the association between skeletal muscle mass and dietary protein intake, habitual physical activity, body composition, and metabolic variables. METHODS: One hundred three healthy postmenopausal women from southern Brazil (age 55.2 ± 4.9 y, body mass index 27.2 ± 4.6 kg/m) were enrolled. Bone mineral density, %body fat, %trunk fat mass, and appendicular lean mass were assessed by dual-energy x-ray absorptiometry, resting metabolic rate by indirect calorimetry, and habitual physical activity by pedometer...
December 5, 2016: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/27922819/early-age-decline-in-dna-repair-capacity-in-the-liver-in-depth-profile-of-differential-gene-expression
#11
Avital Guedj, Anat Geiger-Maor, Eithan Galun, Hagai Amsalem, Jacob Rachmilewitz
Aging is associated with progressive decline in cell function and with increased damage to macromolecular components. DNA damage, in the form of double-strand breaks (DSBs), increases with age and in turn, contributes to the aging process and age-related diseases. DNA strand breaks triggers a set of highly orchestrated signaling events known as the DNA damage response (DDR), which coordinates DNA repair. However, whether the accumulation of DNA damage with age is a result of decreased repair capacity, remains to be determined...
November 30, 2016: Aging
https://www.readbyqxmd.com/read/27922696/down-syndrome-critical-region-1-positively-correlates-with-angiogenesis-in-hypopharyngeal-cancer
#12
Chao Lv, Dayu Liu, Xiaona Wei
Hypopharyngeal carcinoma has one of the highest mortality rates of head and neck cancer, therefore, the identification of markers associated with the pathogenesis and development of hypopharyngeal cancer is critical. Down syndrome critical region 1 (DSCR1) is associated with carcinogenesis and tumor growth in several types of malignancy. Activation of the vascular endothelial growth factor (VEGF) signaling pathway upregulates DSCR1. The aims of the present study were to determine the expression levels of DSCR1 and VEGF‑C in hypopharyngeal cancer, and investigate the association between DSCR1 and angiogenesis in the disease...
December 6, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922689/rb-knockdown-accelerates-bladder-cancer-progression-through-e2f3-activation
#13
Jiang-Ping Wang, Yong Jiao, Cheng-Yuan Wang, Zhi-Bin Xu, Bo Zhang
Bladder cancer is one of the most common cancers diagnosed in the world and leads to significant mortality and morbidity among affected patients. The retinoblastoma (Rb) protein is a main tumor suppressor, controlling cellular responses to potentially oncogenic stimulation. E2F3 was invariably disrupted in different human cancers for its central role in the control of cellular proliferation. Here, we investigated how Rb is integrated to control bladder cancer progression through E2F3 and p53 regulation. The results exhibit that Rb expression is lower in patients with bladder tumor, while E2F3 level is high...
December 6, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27922688/berberine-exerts-an-anti-inflammatory-role-in-ocular-behcet-s-disease
#14
Yan Yang, Qin Wang, Manyun Xie, Ping Liu, Xin Qi, Xiao Liu, Zhuo Li
Behcet's disease is a multi‑system inflammatory disorder, and ocular Behcet's disease (OBD) is one of the most common causes of uveitis in China. A number of studies have indicated that Th17 cells, a subset of interleukin-17 (IL-17)-producing CD4+ T‑helper cells, serve important roles in the pathogenesis of OBD. Berberine (BBR) is an isoquinoline derivative alkaloid isolated from Chinese herbs, and has been used traditionally for the treatment of gastrointestinal disorders. The aim of the present study was to investigate the effect of BBR on Th17 cell proliferation and cytokine secretion, and the expression and activation of the signal transducer and activator of transcription 3 (STAT3) transcription factor in OBD in vitro...
December 5, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922685/5%C3%A2-bromo%C3%A2-3%C3%A2-3%C3%A2-hydroxyprop%C3%A2-1%C3%A2-ynyl-%C3%A2-2h%C3%A2-pyran%C3%A2-2%C3%A2-one-induces-apoptosis-in-t24-human-bladder-cancer-cells-through-mitochondria-dependent-signaling-pathways
#15
Guo-Qiang Yu, Zhong-Ling Dou, Zhao-Hui Jia
The present study was performed to investigate the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one (BHP) on the induction of apoptosis and cell cycle arrest in T24 human bladder carcinoma cells. An MTT assay was used to investigate the inhibition of cell proliferation. Flow cytometry was used to observe alterations in the cell cycle, generation of reactive oxygen species (ROS), alterations in mitochondrial membrane potential (MMP) and induction of apoptosis in the T24 cells following BHP treatment...
December 6, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922684/excess-mechanical-stress-and-hydrogen-peroxide-remodel-extracellular-matrix-of-cultured-human-uterosacral-ligament-fibroblasts-by-disturbing-the-balance-of-mmps-timps-via-the-regulation-of-tgf%C3%A2-%C3%AE-1-signaling-pathway
#16
Qifan Zhang, Cheng Liu, Shasha Hong, Jie Min, Qing Yang, Ming Hu, Yang Zhao, Li Hong
The regulation of the extracellular matrix (ECM) by mechanical stress is of interest as the ECM is essential in the development of pelvic organ prolapse. In the present study, the effect of overexposure to mechanical stress on the ECM, and the probable underlying mechanisms in cultured human uterosacral ligament fibroblasts (hUSLFs), was explored. Mechanical stress has an effect on oxidation‑antioxidation products in parametrial ligament fibroblasts. Thus, hUSLFs were incubated with different concentrations of hydrogen peroxide to elucidate any potential interactions...
December 6, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922683/molecular-inhibition-mechanisms-of-cell-migration-and-invasion-by-coix-polysaccharides-in-a549-nsclc-cells-via-targeting-s100a4
#17
Cheng Luo, Xin Wang, Can An, Chin-Fa Hwang, Wenhua Miao, Lu Yang, Maonian Xu, Aiping Bai, Shanggui Deng
S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non‑small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively...
December 5, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922682/gene-expression-profiles-of-cyp24a1-and-cyp27b1-in-malignant-and-normal-breast-tissues
#18
Naghmeh Zhalehjoo, Yadollah Shakiba, Mojtaba Panjehpour
Active vitamin D has several antitumor effects, including prodifferentiative, antiproliferative and proapoptotic functions in a number of tissues via its binding to vitamin D receptor. The 24‑hydroxylase (CYP24A1) and 1‑hydroxylase (CYP27B1) enzymes are considered to be pivotal determinants of the local concentration of active vitamin D. The aim of the present study was to investigate the mRNA expression levels of the CYP24A1 and CYP27B1 genes in malignant and normal breast tissues. The tumor and adjacent normal tissue samples of 30 patients with breast cancer were acquired from the Iran National Tumor Bank, Imam Hospital (Tehran, Iran)...
December 6, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27922681/cxcr4-promotes-cisplatin-resistance-of-non-small-cell-lung-cancer-in-a-cyp1b1-dependent-manner
#19
Songping Xie, Zhenbo Tu, Jie Xiong, Ganjun Kang, Lina Zhao, Weidong Hu, Haiyan Tan, Kingsley Miyanda Tembo, Qianshan Ding, Xinzhou Deng, Jie Huang, Qiuping Zhang
Chemoresistance is the main cause of treatment failure and high mortality in advanced lung cancer. Cisplatin, an important chemotherapeutic agent for lung cancer, has been observed to show enormously reduced chemotherapeutic efficacy owing to the development of chemoresistance. CXCR4, a stromal-derived-factor-1 specific chemokine receptor, is highly expressed in non-small cell lung cancer (NSCLC) tissues and participates in cancer progression by regulating cell growth, apoptosis or invasion. In this study, we therefore investigated whether CXCR4 plays a role in the cisplatin associated resistance in NSCLC...
December 2, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27922677/prx1-promotes-the-proliferation-and-migration-of-vascular-smooth-muscle-cells-in-a-tlr4-dependent-manner
#20
Zhicheng Zhu, Xiaomei Zheng, Dan Li, Tiance Wang, Rihao Xu, Hulin Piao, Kexiang Liu
The present study aimed to examine the effect of peroxiredocin 1 (Prx1) on vascular smooth muscle cell (VSMC) proliferation, invasion and migration abilities, and to examine the effect of Toll‑like receptor 4 (TLR4) gene silencing on VSMC proliferation, invasion and migration induced by the overexpression of Prx1. The expression of Prx1 in rats with vein graft intimal hyperplasia (IH) was detected using western blot analysis. In addition, VSMCs were retrovirally transfected to establish stable cell lines overexpressing Prx1...
December 6, 2016: Molecular Medicine Reports
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