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https://www.readbyqxmd.com/read/29917317/meta-analysis-of-publicly-available-chinese-hamster-ovary-cho-cell-transcriptomic-datasets-for-identifying-engineering-targets-to-enhance-recombinant-protein-yields
#1
Linas Tamošaitis, C Mark Smales
Transcriptomics has been extensively applied to the investigation of the CHO cell platform for the production of recombinant biotherapeutic proteins to identify transcripts whose expression is regulated and correlated to (non)desirable CHO cell attributes. However, there have been few attempts to analyse the findings across these studies to identify conserved changes and generic targets for CHO cell platform engineering. Here we have undertaken a meta-analysis of CHO cell transcriptomic data and report on those genes most frequently identified as differentially expressed with regard to cell growth (µ) and productivity (Qp)...
June 19, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29917292/effect-of-17%C3%AE-estradiol-on-t-type-calcium-channels-in-the-lateral-habenula
#2
Mei Ying Song, Chun Ying Li, Xiao Feng Liu, Jin Yu Xiao, Hua Zhao
T-type calcium channels (T-channels) are critical for regulating neuronal excitability. Estrogen could alter neuronal excitability by modulating the expression of T-channels. The lateral habenula (LHb) as a link between the limbic system and midbrain structures expresses T-channels and estrogen receptors. However, little is known about the role of estrogen in modulating T-channels in the LHb. In this study, we investigated the distribution of T-channels in three LHb subregions (rostral, middle and caudal) in normal female rats...
June 19, 2018: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/29917261/diagnostic-accuracy-of-immunohistochemical-markers-in-differentiation-between-basal-cell-carcinoma-and-trichoepithelioma-in-small-biopsy-specimens
#3
Naglaa A Mostafa, Magda Assaf, Sami Elhakim, Mona R E Abdel-Halim, Eman El-Nabarawy, Khaled Ghareeb
BACKGROUND: The distinction of trichoepithelioma from basal cell carcinoma in small superficial biopsies is important but often challenging. This has inspired many scientists to test the validity of immunohistochemical markers in the differential diagnosis. OBJECTIVES: to develop an immunohistochemical protocol that helps in differentiation between both TE and BCC with higher sensitivity and specificity. METHODS: Using standard immunohistochemical techniques, we examined 10 TEs and 19 BCCs for the expression of CK19, Ki67, Androgen receptors (AR), CD10, and PHLDA1...
June 19, 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29917258/curcumin-ameliorates-cisplatin-induced-cystopathy-via-activating-nrf2-pathway
#4
Yun Peng Shao, Qiao Zhou, Yun Peng Li, Si Cong Zhang, He Wei Xu, Shuo Wu, Bai Xin Shen, Liu Cheng Ding, Jun Xue, Zheng Sen Chen, Zhong Qing Wei
OBJECTIVES: The present work evaluated preventive effect of curcumin on cisplatin-induced bladder cystopathy. METHODS: Fifteen female rats were divided into (i) Control group administered with physiological saline solution for 5 days; (ii) Cis-P group injected with cisplatin (6 mg/kg); and (iii) Cis-Cur group given cisplatin (6 mg/kg) with curcumin for 5 consecutive days. The function of bladder was measured by means of urodynamic analysis. Furthermore, hematoxylin-eosin staining and Masson trichrome staining were performed for morphological analysis...
June 19, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29917235/classification-of-gabaergic-neuron-subtypes-from-the-globus-pallidus-using-wild-type-and-transgenic-mice
#5
Karina P Abrahao, David M Lovinger
KEY POINTS: Classifying different subtypes of neurons in deep brain structures is a challenge and is crucial to better understanding brain function. Understanding the diversity of neurons in the Globus Pallidus, a brain region positioned to influence afferent and efferent information processing within basal ganglia, could help to explain a variety of brain functions. We present a classification of neurons from the GP using electrophysiological data from wild type mice and confirmation using transgenic mice...
June 19, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29917210/bioinformatics-analysis-of-common-differential-genes-of-coronary-artery-disease-and-ischemic-cardiomyopathy
#6
G-M Li, C-L Zhang, R-P Rui, B Sun, W Guo
OBJECTIVE: This paper aims at screening the common differential genes of coronary atherosclerotic heart disease (CAD) and ischemic cardiomyopathy (ICM), and to conduct pathway analysis and protein-protein interaction (PPI) network analysis for the differential genes. MATERIALS AND METHODS: The CAD and ICM datasets were collected from the Gene Expression Omnibus (GEO) database for human tumors to extract data components of peripheral blood RNA of patients and normal people in GSE71226 and GSE9128 chips; "limma" package of "R" software was used to screen the differential genes, and "pheatmap" package was applied to construct heat maps for the differential genes; Cytoscape, Database for Annotation, Visualization and Integration Discovery (DAVID) and String platforms were utilized for PPI network analysis, Genome Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on the selected differential genes...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917209/mrna-and-protein-expression-of-sarckatp-channel-subunit-kir6-2-after-exercise-induced-myocardial-injury-in-rats
#7
K Wang, R Meng, H Zhang, B-C Xu
OBJECTIVE: To investigate the expression of kir6.2 subunit of the sarcKATP channel in exercise-induced myocardial injury and to elucidate the underlying mechanism of myocardial protection by sarcKATP channels. MATERIALS AND METHODS: Healthy male Sprague Dawley(SD) rats were divided into the Control (C) and the Exhaustive Exercise (EE) group. The one-time exhaustive exercise-induced myocardial injury model was established on a treadmill at a speed of 35 m/min. Alterations in myocardial ischemia and hypoxia were examined by hematoxylin-basic fuchsin-picric acid (HBFP) staining and the concentration of cardiac Troponin I (cTnl), a sensitive and specific marker for myocardial injury, was detected using immunochemiluminescence analysis...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917202/mir-134-expression-and-changes-in-inflammatory-cytokines-of-rats-with-epileptic-seizures
#8
W-S Huang, L Zhu
OBJECTIVE: To investigate the expression of miR-134 and the change of inflammatory cytokines in seizure rats and to explore its relationship. MATERIALS AND METHODS: A rat model of seizures was made by an intraperitoneal injection with kainic acid. ELISA kit for detection of seizures in rats was used. The changes of inflammatory cytokines (IL-1, IL-2, IL-6, TNF-α, IFN-γ) and the hippocampal neuronal cell growth were observed. The expression of miR-134 in brain tissue and serum samples of model group and control group was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) quantitative determination...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917200/analysis-the-effect-of-hyperbaric-oxygen-preconditioning-on-neuronal-apoptosis-ca2-concentration-and-caspases-expression-after-spinal-cord-injury-in-rats
#9
J-Y Pan, R-X Cai, Y Chen, Y Li, W-W Lin, J Wu, X-D Wang
OBJECTIVE: To investigate the effects of hyperbaric oxygen preconditioning (HBO-PC) on neuronal apoptosis, Ca2+ concentration, and Caspases expression after spinal cord injury (SCI) in rats. MATERIALS AND METHODS: A total of 36 rats were randomly divided into control group (CON group), hyperbaric oxygen preconditioning group (HBO-PC group) and spinal cord injury group (SCI group), with 12 rats in each group. Rats in group HBO-PC were given HBO-PC intervention before modeling...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917192/mir-4299-suppresses-non-small-cell-lung-cancer-cell-proliferation-migration-and-invasion-through-modulating-pten-akt-pi3k-pathway
#10
W-B Yang, W-P Zhang, J-L Shi, J-W Wang
OBJECTIVE: The aim of the present study was to investigate clinical significances and biological roles of miR-4299 in non-small cell lung cancer (NSCLC) PATIENTS AND METHODS: Expression of miR-4299 in NSCLC tissues and matched non-tumor tissues was determined by quantitative real-time PCR (qRT-PCR). The correlations between miR-4299 expression and clinicopathological characteristics and prognosis were also analyzed. MTT assay and Transwell assay were performed to determine the proliferation, migration and invasion...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917191/autophagy-regulates-chemoresistance-of-gastric-cancer-stem-cells-via-the-notch-signaling-pathway
#11
L-Q Li, D Pan, S-W Zhang, D -Y-Xie, X-L Zheng, H Chen
OBJECTIVE: Gastric cancer is the most common gastrointestinal malignancy and the leading cause of cancer-related deaths in East Asia. Increasing evidence has revealed that autophagy is closely associated with tumor initiation and progression. The present work aimed to investigate the role of autophagy in adjuvant chemotherapy for gastric cancer. MATERIALS AND METHODS: Gastric cancer stem cells (CSCs) were isolated from gastric cancer cell lines using the cell surface markers CD44 and CD54 and cultured in a three-dimensional cell culture system...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917189/expressions-and-correlation-analysis-of-hif-1%C3%AE-survivin-and-vegf-in-patients-with-hepatocarcinoma
#12
Q-G Tian, Y-T Wu, Y Liu, J Zhang, Z-Q Song, W-F Gao, T-K Guo, C-H He, F-R Dai
OBJECTIVE: To investigate the expressions of HIF-1α, surviving, and VEGF in patients with hepatocarcinoma as well as the correlation analysis among them. PATIENTS AND METHODS: 65 patients, who were admitted to our hospital and diagnosed as hepatocarcinoma from January 2014 to October 2015, were selected as hepatocarcinoma group, while 50 healthy cases that do not have hepatocarcinoma were selected as normal control group. The expression levels of HIF-1α, surviving, and VEGF in hepatocarcinoma tissues of hepatocarcinoma group and normal liver tissues of control group were detected by immunohistochemical (SP) staining method; then, the correlation among them was explored...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917188/mechanism-of-lncrna-duxap8-in-promoting-proliferation-of-bladder-cancer-cells-by-regulating-pten
#13
M-G Lin, Y-K Hong, Y Zhang, B-B Lin, X-J He
OBJECTIVE: To evaluate the lncRNA DUXAP8 expression in bladder cancer and its mechanism. PATIENTS AND METHODS: Clinical specimens were analyzed. The expression of lncRNA in bladder cancer and adjacent tissues was detected using qRT-PCR. The χ2-test analysis was used to analyze the relationship between lncRNA DUXAP8 expression and clinicopathological information in patients with bladder cancer. The tumor cell activity and cell proliferation were measured by cell counting kit-8 (CCK8) and colony formation assay...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917186/long-non-coding-rna-fezf1-as1-is-up-regulated-and-associated-with-poor-prognosis-in-patients-with-cervical-cancer
#14
H-H Zhang, A-H Li
OBJECTIVE: Long noncoding RNA FEZF1-AS1 (FEZF1-AS1) has been showed to involve in a variety of cancers. However, its function and clinical significance in cervical cancer (CC) have not been investigated. The aim of this study was to explore the prognostic value of FEZF1-AS1 in CC patients. PATIENTS AND METHODS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the expression level of FEZF1-AS1 in CC specimens and adjacent normal cervical tissues...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917184/long-noncoding-rna-snhg5-is-up-regulated-and-serves-as-a-potential-prognostic-biomarker-in-acute-myeloid-leukemia
#15
J Li, C-K Sun
OBJECTIVE: Growing evidence has demonstrated that the dysregulation of long non-coding RNAs (lncRNAs) may act as an important role in human tumorigenesis. Our present study aimed to explore the expression pattern and prognostic value of a newly discovered lncRNA small nucleolar RNA host gene 5 (SNHG5) in acute myeloid leukemia (AML). PATIENTS AND METHODS: The expression of SNHG5 was determined using Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in bone marrow and plasma obtained from AML patients and healthy controls...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917183/mir-143-regulates-proliferation-and-apoptosis-of-myelocytic-leukemia-cell-hl-60-via-modulating-erk1
#16
B Song, Y-J Tang, W-G Zhang, C-C Wan, Y Chen, L-J Zhang
OBJECTIVE: Extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) signaling pathway is widely involved in cell proliferation and invasion regulation. Enhanced expression or function of ERK1 is important for leukemia. Abnormal down-regulation of microRNA (miR)-143 is correlated with leukemia pathogenesis, indicating possible tumor-suppressing role. Bioinformatics analysis showed the existence of complementary binding sites between miR-143 and ERK1. This study aims to investigate whether the miR-143 plays a role in mediating ERK1 expression and proliferation and apoptosis of leukemia cells...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917181/mir-29a-promotes-osteogenic-differentiation-of-mesenchymal-stem-cells-via-targeting-hdac4
#17
K Tan, Y-T Peng, P Guo
OBJECTIVE: To investigate the role of miR-29a in regulating the differentiation mesenchymal stem cells into osteoblasts. MATERIALS AND METHODS: For the first step, the changes of expression of miR-29a during the process of mesenchymal stem cells (MSCs) differentiation into osteoblast were detected. Then, we infected the MSCs with mimics or inhibitors of miR-29a to explore the roles of miR-29a in the differentiation. Further, the prediction and verification of the possible target genes of miR-29a were achieved by bioinformatics analysis and luciferase reporter assay...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917168/evaluation-of-anticancer-agents-using-patient-derived-tumor-organoids-characteristically-similar-to-source-tissues
#18
Hirosumi Tamura, Arisa Higa, Hirotaka Hoshi, Gen Hiyama, Nobuhiko Takahashi, Masae Ryufuku, Gaku Morisawa, Yuka Yanagisawa, Emi Ito, Jun-Ichi Imai, Yuu Dobashi, Kiyoaki Katahira, Shu Soeda, Takafumi Watanabe, Keiya Fujimori, Shinya Watanabe, Motoki Takagi
Patient-derived tumor xenograft models represent a promising preclinical cancer model that better replicates disease, compared with traditional cell culture; however, their use is low-throughput and costly. To overcome this limitation, patient-derived tumor organoids (PDOs) were established from human lung, ovarian and uterine tumor tissues, among others, to accurately and efficiently recapitulate the tissue architecture and function. PDOs were able to be cultured for >6 months, and formed cell clusters with similar morphologies to their source tumors...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917164/long-non-coding-rna-bancr-regulates-cancer-stem-cell-markers-in-papillary-thyroid-cancer-via-the-raf-mek-erk-signaling-pathway
#19
Yuanyuan Wang, Xiangde Lin, Xinghao Fu, Wei Yan, Fusheng Lin, Penghao Kuang, Yezhe Luo, Ende Lin, Xiaoquan Hong, Guoyang Wu
Thyroid cancer is one of the most common malignant tumors of the endocrine system. Among all thyroid cancers, papillary thyroid carcinoma (PTC) is the most common type. The BRAF-activated non-coding RNA (BANCR) is a 693-bp nucleotide transcript which was first identified in melanoma. However, the role of BANCR in the development of thyroid cancer remains unclear. Therefore, the present study investigated the potential involvement of BANCR in the development of thyroid cancer in vitro using patient tissue samples and a panel of thyroid cancer cell lines, and in vivo using a xenograft mouse model...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917151/synthesis-of-seed-specific-bidirectional-promoters-for-metabolic-engineering-of-anthocyanin-rich-maize
#20
Xiaoqing Liu, Suzhen Li, Wenzhu Yang, Bona Mu, Yong Jiao, Xiaojin Zhou, Chunyi Zhang, Yunliu Fan, Rumei Chen
Tissue-specific promoters play an important role in plant molecular farming. Here, we describe a strategy to modify the tissue specificity of a maize embryo-specific bidirectional promoter PZmBD1. Six types of cis elements: RY repeats (R), GCN4 (G), the prolamin box (P), Skn-1 (S), and the ACGT and AACA (A) motifs, were collected and fused to PZmBD1 to generate eight chimeric putative bidirectional promoters. Qualitative and quantitative analysis of reporter genes driven by the promoters showed that two promoters exhibited high seed-specific bidirectional activity in maize transient and stable transformed system...
June 15, 2018: Plant & Cell Physiology
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