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Bacteria AND aminoacid metabolism

Mariana Pérez-Ibarreche, Lucía M Mendoza, Graciela Vignolo, Silvina Fadda
Some lactic acid bacteria have the ability to form biofilms on food-industry surfaces and this property could be used to control food pathogens colonization. Lactobacillus sakei CR1862 was selected considering its bacteriocinogenic nature and ability to adhere to abiotic surfaces at low temperatures. In this study, the proteome of L. sakei CRL1862 grown either under biofilm on stainless steel surface and planktonic modes of growth at 10°C, was investigated. Using two-dimensional gel electrophoresis, 29 out of 43 statistically significant spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
October 3, 2017: International Journal of Food Microbiology
Elena Zanni, Emily Schifano, Sara Motta, Fabio Sciubba, Claudio Palleschi, Pierluigi Mauri, Giuditta Perozzi, Daniela Uccelletti, Chiara Devirgiliis, Alfredo Miccheli
Lactobacillus delbrueckii represents a technologically relevant member of lactic acid bacteria, since the two subspecies bulgaricus and lactis are widely associated with fermented dairy products. In the present work, we report the characterization of two commercial strains belonging to L. delbrueckii subspecies bulgaricus, lactis and a novel strain previously isolated from a traditional fermented fresh cheese. A phenomic approach was performed by combining metabolomic and proteomic analysis of the three strains, which were subsequently supplemented as food source to the model organism Caenorhabditis elegans, with the final aim to evaluate their possible probiotic effects...
2017: Frontiers in Microbiology
J A Núñez-Díaz, M Fumanal, E Viguera, M A Moriñigo, M C Balebona
Photobacterium damselae subsp. piscicida (Phdp), the causative agent of photobacteriosis, is an important pathogen in marine aquaculture that affects many different fish species worldwide, including Solea senegalensis, an important fish species for aquaculture in the south of Europe. Bacteria express different repertoires of proteins in response to environmental conditions and when invading a host, sense in vivo environment and adapt by changing the expression of specific proteins. In the case of pathogens, identification of genes with up-regulated expression in vivo compared to in vitro conditions might give an insight into the genes relevant to the bacterial virulence...
May 2017: Fish & Shellfish Immunology
Giuseppina Stellato, Antonietta La Storia, Francesca De Filippis, Giorgia Borriello, Francesco Villani, Danilo Ercolini
UNLABELLED: Microbial contamination in food processing plants can play a fundamental role in food quality and safety. The aims of this study were to learn more about the possible influence of the meat processing environment on initial fresh meat contamination and to investigate the differences between small-scale retail distribution (SD) and large-scale retail distribution (LD) facilities. Samples were collected from butcheries (n = 20), including LD (n = 10) and SD (n = 10) facilities, over two sampling campaigns...
July 1, 2016: Applied and Environmental Microbiology
Marta Borges Canha
BACKGROUND AND AIM: The human colonic mucosa is occupied by a wide range of microorganisms, usually in a symbiotic relation with the host. Sometimes this balance is lost and a state of dysbiosis arises, exposing the colon to different metabolic and inflammatory stimuli (according to the microbiota's changing profile). Recent findings lead to hypothesize that this unbalance may create a subclinical pro-inflammatory state that enables DNA mutations and, therefore, colorectal carcinogenesis...
September 8, 2015: Revista Española de Enfermedades Digestivas
Yuka Norimatsu, Junko Kawashima, Teruko Takano-Yamamoto, Nobuhiro Takahashi
Both Streptococcus and Actinomyces can produce acids from dietary sugars and are frequently found in caries lesions. In the oral cavity, nitrogenous compounds, such as peptides and amino acids, are provided continuously by saliva and crevicular gingival fluid. Given that these bacteria can also utilize nitrogen compounds for their growth, it was hypothesized that nitrogenous compounds may influence their acid production; however, no previous studies have examined this topic. Therefore, the present study aimed to assess the effects of nitrogenous compounds (tryptone and glutamate) on glucose-derived acid production by Streptococcus and Actinomyces...
September 2015: Microbiology and Immunology
Bruna Guida, Mauro Cataldi, Eleonora Riccio, Lucia Grumetto, Andrea Pota, Silvio Borrelli, Andrea Memoli, Francesco Barbato, Gennaro Argentino, Giuliana Salerno, Bruno Memoli
p-Cresol is a by-product of the metabolism of aromatic aminoacid operated by resident intestinal bacteria. In patients with chronic kidney disease, the accumulation of p-cresol and of its metabolite p-cresyl-sulphate causes endothelial dysfunction and ultimately increases the cardiovascular risk of these patients. Therapeutic strategies to reduce plasma p-cresol levels are highly demanded but not available yet. Because it has been reported that the phosphate binder sevelamer sequesters p-cresol in vitro we hypothesized that it could do so also in peritoneal dialysis patients...
2013: PloS One
Catherine P K Chong, Philippa B Mills, Patricia McClean, Paul Gissen, Christopher Bruce, Jens Stahlschmidt, A S Knisely, Peter T Clayton
Born at 27 weeks gestation, a child of consanguineous parents of Pakistani origin required prolonged parenteral nutrition. She developed jaundice, with extensive fibrosis and architectural distortion at liver biopsy; jaundice resolved with supportive care. Serum γ-glutamyl transpeptidase values were within normal ranges. The bile acids in her plasma and urine were >85% unconjugated (non-amidated). Two genes encoding bile-acid amidation enzymes were sequenced. No mutations were found in BAAT, encoding bile acid-CoA : aminoacid N-acyl transferase...
May 2012: Journal of Inherited Metabolic Disease
Hélène Falentin, Stéphanie-Marie Deutsch, Gwenaël Jan, Valentin Loux, Anne Thierry, Sandrine Parayre, Marie-Bernadette Maillard, Julien Dherbécourt, Fabien J Cousin, Julien Jardin, Patricia Siguier, Arnaud Couloux, Valérie Barbe, Benoit Vacherie, Patrick Wincker, Jean-François Gibrat, Claude Gaillardin, Sylvie Lortal
BACKGROUND: Propionibacterium freudenreichii is essential as a ripening culture in Swiss-type cheeses and is also considered for its probiotic use. This species exhibits slow growth, low nutritional requirements, and hardiness in many habitats. It belongs to the taxonomic group of dairy propionibacteria, in contrast to the cutaneous species P. acnes. The genome of the type strain, P. freudenreichii subsp. shermanii CIRM-BIA1 (CIP 103027(T)), was sequenced with an 11-fold coverage. METHODOLOGY/PRINCIPAL FINDINGS: The circular chromosome of 2...
2010: PloS One
Maira Goytia, Nathalie Chamond, Alain Cosson, Nicolas Coatnoan, Daniel Hermant, Armand Berneman, Paola Minoprio
The first eukaryotic proline racemase (PRAC), isolated from the human Trypanosoma cruzi pathogen, is a validated therapeutic target against Chagas' disease. This essential enzyme is implicated in parasite life cycle and infectivity and its ability to trigger host B-cell nonspecific hypergammaglobulinemia contributes to parasite evasion and persistence. Using previously identified PRAC signatures and data mining we present the identification and characterization of a novel PRAC and five hydroxyproline epimerases (HyPRE) from pathogenic bacteria...
2007: PloS One
Nicola Vitulo, Alessandro Vezzi, Chiara Romualdi, Stefano Campanaro, Giorgio Valle
BACKGROUND: Vibrionaceae represent a significant portion of the cultivable heterotrophic sea bacteria; they strongly affect nutrient cycling and some species are devastating pathogens. In this work we propose an improved phylogenetic profile analysis on 14 Vibrionaceae genomes, to study the evolution of this family on the basis of gene content. The phylogenetic profile is based on the observation that genes involved in the same process (e.g. metabolic pathway or structural complex) tend to be concurrently present or absent within different genomes...
2007: BMC Bioinformatics
Agnieszka Sekowska, Valérie Dénervaud, Hiroki Ashida, Karine Michoud, Dieter Haas, Akiho Yokota, Antoine Danchin
BACKGROUND: The thiomethyl group of S-adenosylmethionine is often recycled as methionine from methylthioadenosine. The corresponding pathway has been unravelled in Bacillus subtilis. However methylthioadenosine is subjected to alternative degradative pathways depending on the organism. RESULTS: This work uses genome in silico analysis to propose methionine salvage pathways for Klebsiella pneumoniae, Leptospira interrogans, Thermoanaerobacter tengcongensis and Xylella fastidiosa...
March 4, 2004: BMC Microbiology
S Landi
Glutathione S-transferases (GSTs) are an important part of the cellular detoxification system and, perhaps, evolved to protect cells against reactive oxygen metabolites. Theta is considered the most ancient among the GSTs and theta-like GSTs are found in mammals, fish, insects, plants, unicellular algae, and bacteria. It is thought that an ancestral theta-gene underwent an early duplication before the divergence of fungi and animals and further duplications generated the variety of the other classes of GSTs (alpha, mu, phi, etc...
October 2000: Mutation Research
L Muth
A system for total parenteral nutrition (TPN) with a 3-litre container (big bag) has been developed. The bag is filled by a pharmacist in the hospital pharmacy. One of four standard nutritional regimes can be chosen depending on the patients weight and estimated metabolic condition. The bags contain aminoacids, electrolytes, trace elements and energy in the form of glucose. Lipids with vitamins are given separately via a side port and not mixed into the bags. During one year 61 patients have been given a total of 846 bags...
1985: Acta Anaesthesiologica Scandinavica. Supplementum
R Fensterbank
In an attempt to reproduce resistance to Listeria monocytogenes induced by primo-infection, a vaccinal strain of low virulence capable of affording a good immunity was looked for. After a double mutation in regard to streptomycin, i.e. dependence then reversion to independence, nine strains were obtained from a Listeria monocytogenes field strain of low virulence. In addition, five of these reverse strains were made resistant to erythromycin. Measure of virulence of the resulting 14 strains was performed on mice by spleen counts three days after subcutaneous inoculation of 10(5) bacteria...
1986: Annales de Recherches Vétérinaires. Annals of Veterinary Research
A P Wagner
There is a general consensus that biological specificity is a structure-derived property. If a living system is going to maintain its structure and function then the newly synthesized molecules should replace the faulty ones at the correct time and in the correct places so that the previously established cellular topology will be preserved. In addition, pre-existing spatial determinants which will direct the asymmetrical assembly of the newly synthesized molecules should be available. Therefore, regulation of turnover of cellular architecture represents an essential feature of living systems...
May 22, 1989: Journal of Theoretical Biology
E M Szewczyk
Staphylococci--chemoorganotrophic bacteria whose main habitats are human and animal organisms--can accumulate poly-beta-hydroxybutyrate (PHB) in their cells. The polymer is metabolized in endogenous turnovers preceding degradation of aminoacids, proteins and RNA. PHB depolymerase was not found in staphylococci but beta-hydroxybutyrate dehydrogenase was estimated, purified and characterized.
December 1992: FEMS Microbiology Reviews
A Danchin
The usual models for cell multiplication only involve macromolecular events and ignore the influence of metabolism. The conjecture presented here shows how metabolism could be involved. Separate pathways might cooperate for the synthesis of a small molecule, acting as positive control of several macromolecular syntheses, including stable RNA synthesis. A detailed analysis of the available data showing an influence of metabolites on macromolecular syntheses suggests that two main pathways might cooperate, one involving serine and folic acid metabolism and the second involving threonine and the branched chain aminoacids...
1979: Biochimie
V I Zaĭonts, M V Krylov, V I Loskot, A I Kirillov
The inhibitory analysis has shown that E. tenella does not include ready folic acid into metabolic process but synthesizes its metabolite, 7:8-dihydrofolic acid (DHFA), from its precursor, para-aminobenzoic acid (PABA), which enters the host's organism. PABA can be fully substituted in the process of metabolism by sulphadimesin (SD) at the molar ratio PABA: SD=1: 16.7. As a concurrent antagonist of PABA SD is 6 times stronger in the parasite-host system of Coccidia than in bacteria in cultural medium. The increase in PABA content in relation to SD more than 1: 16...
January 1978: Parazitologiia
R P Naumova, I M Giniatullin, N G Zakharova, N A Shishkova
A peculiarity of the amino acid pool has been discovered for the first time in bacteria utilizing such synthetic compounds as gamma butyrolactam, epsilon-caprolactam and zeta-aminoenanthic acid. The main components of the amino acid pool are omega-aminoacids (including synthetic ones) as well as glutamic acid. The total amino acid content increases upon utilization of the compounds being tested three times (Pseudomonas fluorescens), five times (Pseudomonas dacunchae) and seven times (Pseudomonas perolens) as compared to the control variants...
July 1978: Mikrobiologiia
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