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Peripheral blood gene expression profile predicts stroke

Jianhong Bao, Saijun Zhou, Sipei Pan, Yang Zhang
BACKGROUND: Stroke is one of the leading causes of death worldwide, and ischemic stroke accounts for about 80 percent of all cases. Ischemic stroke is usually caused by a blockage in an artery to the brain. However, its molecular mechanisms remain largely unclear. The integration of mRNA and miRNA expression patterns is an effective strategy to investigate the potential molecular mechanisms of ischemic stroke in posttranscriptional regulation level. METHODS: The mRNA and miRNA expression data were downloaded from the GEO database...
April 1, 2018: Clinical Laboratory
Yang Xue, Pengqi Yin, Guozhong Li, Di Zhong
Several circulating microRNAs (miRNAs) have been proved to serve as stable biomarkers in blood for acute ischemic stroke (AIS). However, the functions of these biomarkers remain elusive. By conducting the integration analysis of circulating miRNAs and peripheral whole-blood mRNAs using bioinformatics methods, we explored the biological role of these circulating markers in peripheral whole blood at the genome-wide level. Stroke-related circulating miRNA profile data (GSE86291) and peripheral whole-blood mRNA expression data (GSE16561) were collected from the Gene Expression Omnibus (GEO) datasets...
June 1, 2018: Neuroscience
George D Vavougios, Sotirios G Zarogiannis, Karen Angeliki Krogfelt, Konstantinos Gourgoulianis, Dimos Dimitrios Mitsikostas, Georgios Hadjigeorgiou
BACKGROUND: currently only 4 studies have explored the potential role of PARK7's dysregulation in MS pathophysiology Currently, no study has evaluated the potential role of the PARK7 interactome in MS. OBJECTIVE: The aim of our study was to assess the differential expression of PARK7 mRNA in peripheral blood mononuclears (PBMCs) donated from MS versus healthy patients using data mining techniques. METHODS: The PARK7 interactome data from the GDS3920 profile were scrutinized for differentially expressed genes (DEGs); Gene Enrichment Analysis (GEA) was used to detect significantly enriched biological functions...
January 2018: Multiple Sclerosis and related Disorders
Kaihua Zhai, Xiangli Kong, Boyu Liu, Jiyu Lou
This study aimed to identify the key differentially expressed genes (DEGs) following ischemic stroke (IS).The GSE22255 microarray dataset, which contains samples from peripheral blood mononuclear cells of 20 IS patients and 20 sex- and age-matched controls, was downloaded from the Gene Expression Omnibus. After data pre-processing, DEGs were identified using the Linear Models for Microarray Data package in R. The Search Tool for the Retrieval of Interacting Genes database was used to predict the interactions among the products of DEGs, and then Cytoscape software was used to visualize the protein-protein interaction (PPI) network...
August 2017: Medicine (Baltimore)
L Perisic, S Aldi, Y Sun, L Folkersen, A Razuvaev, J Roy, M Lengquist, S Åkesson, C E Wheelock, L Maegdefessel, A Gabrielsen, J Odeberg, G K Hansson, G Paulsson-Berne, U Hedin
BACKGROUND: Embolism from unstable atheromas in the carotid bifurcation is a major cause of stroke. Here, we analysed gene expression in endarterectomies from patients with symptomatic (S) and asymptomatic (AS) carotid stenosis to identify pathways linked to plaque instability. METHODS: Microarrays were prepared from plaques (n = 127) and peripheral blood samples (n = 96) of S and AS patients. Gene set enrichment, pathway mapping and network analyses of differentially expressed genes were performed...
March 2016: Journal of Internal Medicine
Shinichi Asano, Paul D Chantler, Taura L Barr
Biomarker profiling is utilized to identify diagnostic and prognostic candidates for stroke. Clinical and preclinical biomarker data suggest altered circulating immune responses may illuminate the mechanisms of stroke recovery. However, the relationship between peripheral blood biomarker profile(s) and brain profiles following stroke remains elusive. Data show that neutrophil lymphocyte ratio (NLR) predicts stroke outcome. Neutrophils release Arginase 1 (ARG1) resulting in T lymphocyte suppression in peripheral blood...
February 2016: Current Opinion in Pharmacology
Taura L Barr, Reyna VanGilder, Stephanie Rellick, Steven D Brooks, Danielle N Doll, Ann Noelle Lucke-Wold, Dongquan Chen, James Denvir, Steven Warach, Andrew Singleton, Mar Matarin
OBJECTIVES: The objectives of this study were to determine the change in gene expression between two time points following stroke and to identify biomarkers of stroke recovery through gene expression profiling and pathway analysis. METHODS: Peripheral blood was collected from 34 ischemic stroke patients (confirmed by magnetic resonance imaging) ≥18 years of age, within 24 hr of symptom onset and 24-48 hr later, and from healthy controls. The Modified Rankin Scale (MRS) was used to determine 30-day recovery...
May 2015: Biological Research for Nursing
Glen C Jickling, Boryana Stamova, Bradley P Ander, Xinhua Zhan, Dazhi Liu, Shara-Mae Sison, Piero Verro, Frank R Sharp
BACKGROUND AND PURPOSE: The cause of ischemic stroke remains unclear, or cryptogenic, in as many as 35% of patients with stroke. Not knowing the cause of stroke restricts optimal implementation of prevention therapy and limits stroke research. We demonstrate how gene expression profiles in blood can be used in conjunction with a measure of infarct location on neuroimaging to predict a probable cause in cryptogenic stroke. METHODS: The cause of cryptogenic stroke was predicted using previously described profiles of differentially expressed genes characteristic of patients with cardioembolic, arterial, and lacunar stroke...
August 2012: Stroke; a Journal of Cerebral Circulation
Lasse Folkersen, Jonas Persson, Johan Ekstrand, Hanna E Agardh, Göran K Hansson, Anders Gabrielsen, Ulf Hedin, Gabrielle Paulsson-Berne
Classic risk factors, including age, smoking, serum cholesterol, diabetes and blood pressure, constitute the basis of present risk prediction models but fail to identify all individuals at risk. The objective of this study was to investigate if genomic and transcriptional patterns improve prediction of ischemic events in patients with established carotid artery disease. Genotype and gene expression profiles were obtained from carotid plaque tissue (n = 126) and peripheral blood mononuclear cells (n = 97) of patients undergoing carotid endarterectomy...
2012: Molecular Medicine
Huichun Xu, Yang Tang, Da-Zhi Liu, Ruiqiong Ran, Bradley P Ander, Michelle Apperson, Xin She Liu, Jane C Khoury, Jeffrey P Gregg, Arthur Pancioli, Edward C Jauch, Kenneth R Wagner, Piero Verro, Joseph P Broderick, Frank R Sharp
There are no biomarkers that differentiate cardioembolic from large-vessel atherosclerotic stroke, although the treatments differ for each and approximately 30% of strokes and transient ischemic attacks have undetermined etiologies using current clinical criteria. We aimed to define gene expression profiles in blood that differentiate cardioembolic from large-vessel atherosclerotic stroke. Peripheral blood samples were obtained from healthy controls and acute ischemic stroke patients (<3, 5, and 24 h). RNA was purified, labeled, and applied to Affymetrix Human U133 Plus 2...
July 2008: Journal of Cerebral Blood Flow and Metabolism
Frank R Sharp, Huichun Xu, Lisa Lit, Wynn Walker, Michelle Apperson, Donald L Gilbert, Tracy A Glauser, Brenda Wong, Andrew Hershey, Da-Zhi Liu, Joseph Pinter, Xinhua Zhan, Xinshe Liu, Ruiqiong Ran
Sequencing of the human genome and new microarray technology make it possible to assess all genes on a single chip or array. Recent studies show different patterns of gene expression related to different tissues and diseases, and these patterns of gene expression are beginning to be used for diagnosis and treatment decisions in various types of lymphoid and solid malignancies. Because of obvious problems obtaining brain tissue, progress in genomics of neurological diseases has been slow. To address this, we demonstrated that different types of acute injury in rodent brain produced different patterns of gene expression in peripheral blood...
November 2006: Archives of Neurology
Alison E Baird
Identifying blood biomarkers may be of particular value in neurologic disorders such as stroke because of the difficulty in directly studying the brain and its blood vessels. Markers of brain injury, inflammation, excitotoxicity, and oxidative damage have been evaluated for their value in stroke diagnosis, treatment, and management, but none have proved to be sensitive or specific enough for routine clinical use. However, new cellular and molecular profiling approaches using the peripheral blood offer the potential for identifying panels of genes and proteins by increasing specificity while maintaining sensitivity...
July 2006: Current Atherosclerosis Reports
Alison E Baird, Violet L Wright
Our understanding of the mechanisms underlying cerebrovascular atherosclerosis has improved in recent years, but significant gaps remain. New insights into the vascular biological processes that result in ischemic stroke may come from cellular and molecular profiling studies of the peripheral blood. In recent cellular profiling studies, increased levels of a proinflammatory T-cell subset (CD4 (+)CD28 (-)) have been associated with stroke recurrence and death. Expansion of this T-cell subset may occur after ischemic stroke and be a pathogenic mechanism leading to recurrent stroke and death...
February 2006: Seminars in Neurology
David F Moore, Hong Li, Neal Jeffries, Violet Wright, Ronald A Cooper, Abdel Elkahloun, Monique P Gelderman, Enrique Zudaire, Gregg Blevins, Hua Yu, Ehud Goldin, Alison E Baird
BACKGROUND: Direct brain biopsy is rarely indicated during acute stroke. This study uses peripheral blood mononuclear cells (PBMCs) to determine whether a systemic gene expression profile could be demonstrated in patients with acute ischemic stroke. METHODS AND RESULTS: Using oligonucleotide microarrays, we compared the gene expression profile of an index cohort of 20 patients with confirmed ischemic stroke on neuroimaging studies with that of 20 referent subjects...
January 18, 2005: Circulation
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