keyword
https://read.qxmd.com/read/38527916/rfxcas13d-mediated-inhibition-of-circ1647-alleviates-renal-fibrosis-via-pi3k-akt-signaling-pathway
#21
JOURNAL ARTICLE
Yufang Ni, Hongmin Zhang, Qianwen Xian, Wenjie Qin, Hongwei Su, Li Wang, Jianchun Li
BACKGROUND: Circular RNAs ( CircRNAs ) have been shown to be involved in the development of chronic kidney disease (CKD). This study aimed to investigate the role of Circ1647 in renal fibrosis, which is a hallmark of CKD. METHODS: In this study, we established a unilateral ureteral obstruction (UUO) model and delivered Circ1647 RfxCas13d knockdown plasmid into renal parenchymal cells via retrograde injection through the ureter followed by electroporation. After that, the pathological changes were determined by Hematoxylin and Eosin...
December 2024: Renal Failure
https://read.qxmd.com/read/38526708/role-of-c-ebp-homologous-protein-in-vascular-stenosis-after-carotid-artery-injury
#22
JOURNAL ARTICLE
Lin Teng, Qin Qin, Zi-Yi Zhou, Fei Zhou, Cun-Yu Cao, Chao He, Jia-Wang Ding, Jian Yang
The study aims to explore the fluctuating expression of C/EBP Homologous Protein (CHOP) following rat carotid artery injury and its central role in vascular stenosis. Using in vivo rat carotid artery injury models and in vitro ischemia and hypoxia cell models employing human aortic endothelial cells (HAECs) and vascular smooth muscle cells (T/G HA-VSMCs), a comprehensive investigative framework was established. Histological analysis confirmed intimal hyperplasia in rat models. CHOP expression in vascular tissues was assessed using Western blot and immunohistochemical staining, and its presence in HAECs and T/G HA-VSMCs was determined through RT-PCR and Western blot...
March 25, 2024: Biochemical Genetics
https://read.qxmd.com/read/38526593/trans-2-nonadecyl-4-hydroxymethyl-1-3-dioxolane-tnhd-purified-from-freshwater-clams-markedly-alleviates-dimethylnitrosamine-induced-hepatic-fibrosis
#23
JOURNAL ARTICLE
Ya-Ru Kuo, Chen-Yu Tsai, Wei-Sheng Lin, Chi-I Chang, Ching-Shu Lai, Min-Hsiung Pan
Liver fibrosis occurs due to injury or inflammation, which results in the excessive production of collagen and the formation of fibrotic scar tissue that impairs liver function. Despite the limited treatment options available, freshwater clams may hold promise in the treatment of liver fibrosis. In this study, we demonstrated the effects of ethanol extract of freshwater clam (FCE), ethyl acetate extract of FCE (EA-FCE), and trans-2-nonadecyl-4-(hydroxymethyl)-1,3-dioxolane (TNHD) on liver fibrosis induced by dimethylnitrosamine (DMN)...
March 15, 2024: Journal of Food and Drug Analysis
https://read.qxmd.com/read/38526390/restoration-of-pregnancy-function-using-gt-pcl-biofilm-in-a-rabbit-model-of-uterine-injury
#24
JOURNAL ARTICLE
Di Huang, Jing Liu, Jie Yang, Junhui Liang, Jing Zhang, Qinyu Han, Jianlong Yu, Tingting Yang, Qi Meng, Thorsten Steinberg, Zhongle Chang, Changzhong Li
Biomaterial scaffolds have been used successfully to promote the regenerative repair of small endometrial lesions in small rodents, providing partial restoration of gestational function. The use of rabbits in this study allowed us to investigate a larger endometrial tissue defect and myometrial-injury model. A gelatin/polycaprolactone (GT/PCL) gradient-layer biofilm was sutured at the defect to guide the reconstruction of the original tissue structure. 28 days post-implantation, the uterine cavity had been restored its original morphology; endometrial growth was accompanied by the formation of glands and blood vessels, and the fragmented myofibers of the uterine smooth muscle had begun to resemble the normal structure of the lagomorph uterine cavity, arranging in a circular luminal pattern and a longitudinal serosal pattern...
March 25, 2024: Tissue Engineering. Part A
https://read.qxmd.com/read/38526145/hyaluronidase-inhibitor-sha2-75-alleviates-ischemia-reperfusion-induced-acute-kidney-injury
#25
JOURNAL ARTICLE
Yang Zhang, Huajiang Zhao, Jing Zhang
Hyaluronidases (HAases) are enzymes that degrade hyaluronic acid (HA) in the animal kingdom. The HAases-HA system is crucial for HA homeostasis and plays a significant role in biological processes and extracellular matrix (ECM)-related pathophysiological conditions. This study aims to explore the role of inhibiting the HAases-HA system in acute kidney injury (AKI). We selected the potent inhibitor "sHA2.75" to inhibit HAase activity through mixed inhibitory mechanisms. The ischemia-reperfusion mouse model was established using male BALB/c mice (7-9 weeks old), and animals were subjected to subcapsular injection with 50 mg/kg sHA2...
March 25, 2024: Cell Cycle
https://read.qxmd.com/read/38525887/comapping-cellular-content-and-extracellular-matrix-with-hemodynamics-in-intact-arterial-tissues-using-scanning-immunofluorescent-multiphoton-microscopy
#26
JOURNAL ARTICLE
Yasutaka Tobe, Anne M Robertson, Mehdi Ramezanpour, Juan R Cebral, Simon C Watkins, Fady T Charbel, Sepideh Amin-Hanjani, Alexander K Yu, Boyle C Cheng, Henry H Woo
Deviation of blood flow from an optimal range is known to be associated with the initiation and progression of vascular pathologies. Important open questions remain about how the abnormal flow drives specific wall changes in pathologies such as cerebral aneurysms where the flow is highly heterogeneous and complex. This knowledge gap precludes the clinical use of readily available flow data to predict outcomes and improve treatment of these diseases. As both flow and the pathological wall changes are spatially heterogeneous, a crucial requirement for progress in this area is a methodology for acquiring and comapping local vascular wall biology data with local hemodynamic data...
March 25, 2024: Microscopy and Microanalysis
https://read.qxmd.com/read/38525665/the-developmental-architecture-of-the-crop-of-quail-embryos-coturnix-coturnix-morphometric-light-and-scanning-electron-microscopy
#27
JOURNAL ARTICLE
Samara Mohamed Ali, Salma A Mohamed, Mohamed A A Mahdy, Fatma A Madkour
The present study focused on the histogenesis of the crop in quail embryos. The developmental sequence was recorded from day 10 of incubation to the hatching day (17th day) by gross anatomy, morphometric analysis and by using light and scanning electron microscope (SEM). Grossly, the crop was distinguished as an enlarged pouch of the cervical oesophagus on the 12th day of incubation. Histologically, the development of the crop began on the 10th day of incubation. The muscularis mucosae was observed as a small, thin layer of myoblast cells on the 10th day, then it appeared as longitudinally arranged smooth muscle fibres with progression of the developmental age till hatching day...
May 2024: Anatomia, Histologia, Embryologia
https://read.qxmd.com/read/38525531/optimization-of-mouse-kidney-digestion-protocols-for-single-cell-applications
#28
JOURNAL ARTICLE
Jake N Robertson, Henry Diep, Alexander Pinto, Christopher G Sobey, Grant R Drummond, Antony Vinh, Maria Jelinic
Single-cell technologies such as flow cytometry and single-cell RNA sequencing (scRNAseq) have allowed for comprehensive characterisation of the kidney cellulome. However, there is disparity in the various protocols for preparing kidney single-cell suspensions. We aimed to address this limitation by characterising kidney cellular heterogeneity using three previously published single-cell preparation protocols. Single-cell suspensions were prepared from male and female C57BL/6 kidneys using the following kidney tissue dissociation protocols: (P1) a scRNAseq protocol; (P2) a multi-tissue digestion kit from Miltenyi Biotec ; and (P3) a protocol established in our laboratory...
March 25, 2024: Physiological Genomics
https://read.qxmd.com/read/38524547/potential-actions-of-capsaicin-for-preventing-vascular-calcification-of-vascular-smooth-muscle-cells-in-vitro-and-in-vivo
#29
JOURNAL ARTICLE
Yin-Fang Yan, Yue Feng, Si-Min Wang, Fei Fang, Hong-Yan Chen, Ming-Xia Zhen, Yu-Qiang Ji, Song-Di Wu
Vascular calcification (VC) is an accurate risk factor and predictor of adverse cardiovascular events; however, there is currently no effective therapy to specifically prevent VC progression. Capsaicin (Cap) is a bioactive alkaloid isolated from Capsicum annuum L., a traditional medicinal and edible plant that is beneficial for preventing cardiovascular diseases. However, the effect of Cap on VC remains unclear. This study aimed to explore the effects and related mechanisms of Cap on aortic calcification in a mouse and on Pi-induced calcification in vascular smooth muscle cells (VSMCs)...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38524279/nf-kb-affects-migration-of-vascular-smooth-muscle-cells-after-treatment-with-heparin-and-ibrutinib
#30
JOURNAL ARTICLE
Nafiseh Shokri, Ghasem Ghasempour, Ali Akbar Soleimani, Mohammad Elahimanesh, Mohammad Najafi
The migration of vascular smooth muscle cells (VSMCs) is one of the most important events in the remodeling of atherosclerosis plaque. The aim of study was to investigate the role of Heparin in the VSMC migration and its association with the NF-kB, collagen 1 and collagen 3 expression levels. Moreover, the incorporation of Heparin was studied in the VSMC cultures including Betulinic acid and Ibrutinib. Twelve cell groups were cultured and treated with the Heparin, Betulinic acid and Ibrutinib based on the viability and toxicity in 24-h and 48-h periods...
July 2024: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/38524234/circulating-mir-129-3p-in-combination-with-clinical-factors-predicts-vascular-calcification-in-hemodialysis-patients
#31
JOURNAL ARTICLE
Jingjing Jin, Meijuan Cheng, Xueying Wu, Haixia Zhang, Dongxue Zhang, Xiangnan Liang, Yuetong Qian, Liping Guo, Shenglei Zhang, Yaling Bai, Jinsheng Xu
BACKGROUND: Vascular calcification (VC) commonly occurs and seriously increases the risk of cardiovascular events and mortality in patients with hemodialysis. For optimizing individual management, we will develop a diagnostic multivariable prediction model for evaluating the probability of VC. METHODS: The study was conducted in four steps. First, identification of miRNAs regulating osteogenic differentiation of vascular smooth muscle cells (VSMCs) in calcified condition...
March 2024: Clinical Kidney Journal
https://read.qxmd.com/read/38523717/a-case-of-ebv-associated-adrenal-leiomyoma
#32
Takumasa Takemoto, Ikuko Kiriyama, Yoshifumi Sugawara, Chie Abe, Norihiro Teramoto, Katsuyoshi Hashine, Isao Yoshida, Akinori Asagi
Adrenal leiomyomas are rare and often reported as Epstein-Barr virus (EBV)-associated smooth muscle tumor (SMT) in association with EBV infection in immunocompromised patients. We experienced a case of right adrenal leiomyoma that was incidentally found in a man in his 70s. Computed Tomography (CT) showed a well-circumscribed mass of 3.1 cm in diameter in the right adrenal gland, which increased to 4.9 cm in diameter over 1 year. Preoperative diagnosis was difficult due to the lack of specific imaging findings...
June 2024: Radiology Case Reports
https://read.qxmd.com/read/38523689/fto-stabilizes-mis12-to-inhibit-vascular-smooth-muscle-cell-senescence-in-atherosclerotic-plaque
#33
JOURNAL ARTICLE
Jingzhao Sun, Mengqi Wang, Fengming Jia, Jiantao Song, Jinlin Ren, Bo Hu
PURPOSE: Atherosclerosis is the main cause of atherosclerotic cardiovascular disease (CVD). Here, we aimed to uncover the role and mechanisms of fat mass and obesity-associated genes (FTO) in the regulation of vascular smooth muscle cell (VSMC) senescence in atherosclerotic plaques. METHODS: ApoE-/- mice fed a high-fat diet (HFD) were used to establish an atherosclerotic animal model. Immunohistochemistry, and the staining of hematoxylin-eosin, Oil Red O, Sirius red, and Masson were performed to confirm the role of FTO in atherosclerosis in vivo...
2024: Journal of Inflammation Research
https://read.qxmd.com/read/38522069/mafb-in-macrophages-regulates-cold-induced-neuronal-density-in-brown-adipose-tissue
#34
JOURNAL ARTICLE
Manoj Kumar Yadav, Megumi Ishida, Natalia Gogoleva, Ching-Wei Liao, Filiani Natalia Salim, Maho Kanai, Akihiro Kuno, Takuto Hayashi, Zeynab Javanfekr Shahri, Kaushalya Kulathunga, Omar Samir, Wenxin Lyu, Olivia Olivia, Evaristus C Mbanefo, Satoru Takahashi, Michito Hamada
Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f ::LysM-Cre) mice. We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions. This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT...
March 22, 2024: Cell Reports
https://read.qxmd.com/read/38520641/verrucous-gastritis-like-lesion-in-intramucosal-helicobacter-pylori-uninfected-signet-ring-cell-carcinoma-with-poorly-differentiated-adenocarcinoma
#35
JOURNAL ARTICLE
Hiroki Takemoto, Takahiro Kotachi, Hajime Teshima, Hirosato Tamari, Akiyoshi Tsuboi, Hidenori Tanaka, Ken Yamashita, Yuji Urabe, Akira Ishikawa, Shiro Oka
In Japan, accessible Helicobacter pylori (Hp) eradication therapy is associated with an increase in the prevalence of gastric cancers (GCs) in Hp uninfected stomachs. Signet ring cell carcinoma (SRCC) is the most common of these GCs. Intramucosal SRCC with poorly differentiated adenocarcinoma (PDA) occurring in Hp uninfected gastric mucosa is rare; furthermore, many Hp uninfected pure SRCCs exhibit discoloration and flat or slightly depressed lesions, and morphological elevation is relatively rare. We report a case of intramucosal SRCC with PDA with an elevated, verrucous gastritis-like lesion in a 57-year-old male patient...
March 23, 2024: Clinical Journal of Gastroenterology
https://read.qxmd.com/read/38519940/total-iridoid-glycoside-extract-of-lamiophlomis-rotata-benth-kudo-accelerates-diabetic-wound-healing-by-the-nrf2-cox2-axis
#36
JOURNAL ARTICLE
Xiaoyu Geng, Ying Wang, Huan Li, Liang Song, Chen Luo, Xiaojie Gu, Haixin Zhong, Huilin Chen, Xinzhu Chen, Jianwei Wang, Zheng Pan
BACKGROUND: Lamiophlomis rotata (Benth.) Kudo (L. rotata), the oral Traditional Tibetan herbal medicine, is adopted for treating knife and gun wounds for a long time. As previously demonstrated, total iridoid glycoside extract of L. rotata (IGLR) induced polarization of M2 macrophage to speed up wound healing. In diabetic wounds, high levels inflammatory and chemotactic factors are usually related to high reactive oxygen species (ROS) levels. As a ROS target gene, nuclear factor erythroid 2-related factor 2 (NRF2), influences the differentiation of monocytes to M1/M2 macrophages...
March 22, 2024: Chinese Medicine
https://read.qxmd.com/read/38519881/vascular-smooth-muscle-cells-in-response-to-cholesterol-crystals-modulates-inflammatory-cytokines-release-and-promotes-neutrophil-extracellular-trap-formation
#37
JOURNAL ARTICLE
Jishamol Thazhathveettil, Ashok Kumar Kumawat, Isak Demirel, Allan Sirsjö, Geena Varghese Paramel
BACKGROUND: The formation and accumulation of cholesterol crystals (CC) at the lesion site is a hallmark of atherosclerosis. Although studies have shown the importance of vascular smooth muscle cells (VSMCs) in the disease atherosclerosis, little is known about the molecular mechanism behind the uptake of CC in VSMCs and their role in modulating immune response. METHODS: Human aortic smooth muscle cells were cultured and treated with CC. CC uptake and CC mediated signaling pathway and protein induction were studied using flow cytometry, confocal microscopy, western blot and Olink proteomics...
March 22, 2024: Molecular Medicine
https://read.qxmd.com/read/38519058/digital-spatial-proteomic-profiling-reveals-immune-checkpoints-as-biomarkers-in-lymphoid-aggregates-and-tumor-microenvironment-of-desmoplastic-melanoma
#38
JOURNAL ARTICLE
David G Su, David A Schoenfeld, Wael Ibrahim, Raysa Cabrejo, Dijana Djureinovic, Raymond Baumann, David L Rimm, Sajid A Khan, Ruth Halaban, Harriet M Kluger, Kelly Olino, Anjela Galan, James Clune
BACKGROUND: Desmoplastic melanoma (DM) is a rare melanoma subtype characterized by dense fibrous stroma, a propensity for local recurrence, and a high response rate to programmed cell death protein 1 (PD-1) blockade. Occult sentinel lymph node positivity is significantly lower in both pure and mixed DM than in conventional melanoma, underscoring the need for better prognostic biomarkers to inform therapeutic strategies. METHODS: We assembled a tissue microarray comprising various cores of tumor, stroma, and lymphoid aggregates from 45 patients with histologically confirmed DM diagnosed between 1989 and 2018...
March 21, 2024: Journal for Immunotherapy of Cancer
https://read.qxmd.com/read/38518636/gualou-xiebai-herb-pair-and-its-active-ingredients-act-against-atherosclerosis-by-suppressing-vsmc-derived-foam-cell-formation-via-regulating-p2ry12-mediated-lipophagy
#39
JOURNAL ARTICLE
Youli Bao, Li Zhu, Yuting Wang, Jiahui Liu, Zijian Liu, Zhenglong Li, An Zhou, Hongfei Wu
BACKGROUND: Atherosclerosis (AS) is a chronic disease characterized by lipid accumulation in the aortic wall and the formation of foam cells overloaded with large lipids inclusions. Currently, Western medicine is primarily used to improve lipid metabolism disorders and reduce inflammatory reactions to delay AS progression, but these medicines come with serious side effects and drug resistance. Gualou-Xiebai (GLXB) is a renowned herb pair that has been proven effective against AS. However, the potential molecular mechanism through which GLXB exerts the anti-atherosclerotic effects of increasing lipophagy in vascular smooth muscle cells (VSMCs) remains unknown...
January 7, 2024: Phytomedicine
https://read.qxmd.com/read/38515647/early-postnatal-moderate-catch%C3%A2-up-growth-in-rats-with-nutritional-intrauterine-growth-restriction-preserves-pulmonary-vascular-and-cognitive-function-in-adulthood
#40
JOURNAL ARTICLE
Lixia Ye, Yajie Huang, Kewei Chen, Chengcheng Hang, Yuhan Ying, Lu Zu, Xiaofei Luo, Lizhong Du
Intrauterine growth restriction (IUGR) with rapid postnatal catch-up growth is strongly associated with pulmonary vascular dysfunction in adulthood, whereas IUGR with delayed growth in early postnatal life results in long-term brain deficits. In the present study, it was hypothesized that IUGR with early moderate catch-up growth may alleviate pulmonary vascular remodeling in adulthood without affecting memory function. An IUGR model was established by restricting maternal nutrition during pregnancy. Different growth patterns were achieved by adjusting the litter size in each group during lactation...
May 2024: Experimental and Therapeutic Medicine
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