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Lung epithelial cells

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https://www.readbyqxmd.com/read/28719656/phenotypic-characterization-of-circulating-tumor-cells-in-the-peripheral-blood-of-patients-with-small-cell-lung-cancer
#1
Ippokratis Messaritakis, Eleni Politaki, Athanasios Kotsakis, Eleftheria-Kleio Dermitzaki, Filippos Koinis, Eleni Lagoudaki, Anastasios Koutsopoulos, Galatea Kallergi, John Souglakos, Vassilis Georgoulias
BACKGROUND: To evaluate the phenotypic heterogeneity of circulating tumor cells (CTCs) based on the expression of proliferative, apoptotic and Epithelial-to-Mesenchymal Transmission (EMT) markers during front-line treatment in patients with small cell lung cancer (SCLC) and to evaluate their clinical relevance. METHODS: CTCs from 108 chemotherapy-naïve patients with SCLC were analyzed by double immunofluorescence staining using anti-Ki67, anti-M30, anti-Vimentin along with anti-CKs antibodies...
2017: PloS One
https://www.readbyqxmd.com/read/28716119/stat1-dependent-and-independent-pulmonary-allergic-and-fibrogenic-responses-in-mice-after-exposure-to-tangled-versus-rod-like-multi-walled-carbon-nanotubes
#2
Katherine S Duke, Alexia J Taylor-Just, Mark D Ihrie, Kelly A Shipkowski, Elizabeth A Thompson, Erinn C Dandley, Gregory N Parsons, James C Bonner
BACKGROUND: Pulmonary toxicity of multi-walled carbon nanotubes (MWCNTs) is influenced by physicochemical characteristics and genetic susceptibility. We hypothesized that contrasting rigidities of tangled (t) versus rod-like (r) MWCNTs would result in differing immunologic or fibrogenic responses in mice and that these responses would be exaggerated in transgenic mice lacking the signal transducer and activator of transcription-1 (STAT1), a susceptible mouse model of pulmonary fibrosis...
July 17, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28716088/loss-of-dip2c-in-rko-cells-stimulates-changes-in-dna-methylation-and-epithelial-mesenchymal-transition
#3
Chatarina Larsson, Muhammad Akhtar Ali, Tatjana Pandzic, Anders M Lindroth, Liqun He, Tobias Sjöblom
BACKGROUND: The disco-interacting protein 2 homolog C (DIP2C) gene is an uncharacterized gene found mutated in a subset of breast and lung cancers. To understand the role of DIP2C in tumour development we studied the gene in human cancer cells. METHODS: We engineered human DIP2C knockout cells by genome editing in cancer cells. The growth properties of the engineered cells were characterised and transcriptome and methylation analyses were carried out to identify pathways deregulated by inactivation of DIP2C...
July 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28716029/foxp3-promotes-tumor-growth-and-metastasis-by-activating-wnt-%C3%AE-catenin-signaling-pathway-and-emt-in-non-small-cell-lung-cancer
#4
Shucai Yang, Yi Liu, Ming-Yue Li, Calvin S H Ng, Sheng-Li Yang, Shanshan Wang, Chang Zou, Yujuan Dong, Jing Du, Xiang Long, Li-Zhong Liu, Innes Y P Wan, Tony Mok, Malcolm J Underwood, George G Chen
BACKGROUND: The role of cancer cell FOXP3 in tumorigenesis is conflicting. We aimed to study FOXP3 expression and regulation, function and clinical implication in human non-small cell lung cancer (NSCLC). METHODS: One hundred and six patients with histologically-confirmed NSCLC who underwent surgery were recruited for the study. Tumor samples and NSCLC cell lines were used to examine FOXP3 and its related molecules. Various cell functions related to tumorigenesis were performed...
July 17, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28716020/long-non-coding-rna-casc2-suppresses-epithelial-mesenchymal-transition-of-hepatocellular-carcinoma-cells-through-casc2-mir-367-fbxw7-axis
#5
Yufeng Wang, Zhikui Liu, Bowen Yao, Qing Li, Liang Wang, Cong Wang, Changwei Dou, Meng Xu, Qingguang Liu, Kangsheng Tu
BACKGROUND: Recently, it has been reported that long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2), a novel tumor suppressor, participates in regulating the carcinogenesis and suppresses tumor progression by sponging microRNAs (miRNAs). However, the expression and function of CASC2 in hepatocellular carcinoma (HCC) remain unclear. METHODS: The expression of CASC2 and miR-367 in HCC specimens and cell lines were detected by real-time PCR. Western blotting and immunohistochemistry were carried out for detection of epithelial-to-mesenchymal transition (EMT) markers in HCC...
July 17, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28715819/effect-of-microrna-135a-on-cell-proliferation-migration-invasion-apoptosis-and-tumor-angiogenesis-through-the-igf-1-pi3k-akt-signaling-pathway-in-non-small-cell-lung-cancer
#6
Yufei Zhou, Shaoxia Li, Jiangtao Li, Dongfeng Wang, Quanxing Li
OBJECTIVE: This study explored the ability of microRNA-135a (miR-135a) to influence cell proliferation, migration, invasion, apoptosis and tumor angiogenesis through the IGF-1/PI3K/Akt signaling pathway in non-small cell lung cancer (NSCLC). METHODS: NSCLC tissues and adjacent normal tissues were collected from 138 NSCLC patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-135a and IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 mRNA; western blotting was used to determine the expression levels of IGF-1, PI3K and Akt protein; and enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression levels of VEGF, bFGF and IL-8 protein...
July 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28715175/airborne-nanoparticle-release-and-toxicological-risk-from-metal-oxide-coated-textiles-toward-a-multi-scale-safe-by-design-approach
#7
Paride Mantecca, Kaja Kasemets, Archana Deokar, Ilana Perelshtein, Aharon Gedanken, Yeon Kyoung Bahk, Baharh Kianfar, Jing Wang
Nano metal oxides have been proposed as alternatives to Silver (Ag) nanoparticles (NPs) for antibacterial coatings. Here cotton and polyester-cotton fabrics were sonochemically coated with Zinc oxide (ZnO) ZnO and Copper oxide (CuO) CuO NPs. By varying the reaction solvent (water or ethanol), NPs with different sizes and shapes were synthesized. The cytotoxic and pro-inflammatory effects of studied NPs were investigated in vitro in human alveolar epithelial A549 and macrophage-like THP1 cells. To understand the potential respiratory impact of the NPs, the coated textiles were subjected to the abrasion tests and the released airborne particles were measured...
July 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28714960/bin1-reverses-pd-l1-mediated-immune-escape-by-inactivating-the-c-myc-and-egfr-mapk-signaling-pathways-in-non-small-cell-lung-cancer
#8
J Wang, Y Jia, S Zhao, X Zhang, X Wang, X Han, Y Wang, M Ma, J Shi, L Liu
Non-small cell lung cancer (NSCLC) is one of the most common and malignant carcinoma worldwide, and the incidence and mortality are increasing rapidly. Immunotherapy targeting programmed death 1/programmed death ligand 1 (PD-L1) signaling has shown prominent clinical effects in treating NSCLC; however, a poor understanding of the associated regulating molecular mechanisms of PD-L1 has become one of the biggest obstacles for further improving efficacy. Bridging integrator-1 (BIN1) can regulate numerous cancer-related molecules to exert multiple tumor-suppressing effects by either interacting or not interacting with c-MYC...
July 17, 2017: Oncogene
https://www.readbyqxmd.com/read/28714804/cellular-proliferation-and-differentiation-induced-by-single-layer-molybdenum-disulfide-and-mediation-mechanisms-of-proteins-via-the-akt-mtor-p70s6k-signaling-pathway
#9
Wei Zou, Xingli Zhang, Mengyang Zhao, Qixing Zhou, Xiangang Hu
Single-layer molybdenum disulfide (SLMoS2) is a novel kind of 2D nanosheet that has attracted great attention regarding its use in biosensors, drug delivery, tissue engineering and therapy. However, our results demonstrated that SLMoS2 accelerated proliferation and promoted myogenic differentiation and epithelial-mesenchymal transition (EMT) in human embryonic lung fibroblasts (HELFs). The abnormal proliferation and differentiation of HELFs contribute to idiopathic pulmonary fibrosis. Specifically, SLMoS2 significantly stimulated the expression of myofibroblast- and mesenchymal-associated genes and proteins...
July 17, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28713982/protective-effects-of-socs3-overexpression-in-high-glucose%C3%A2-induced-lung-epithelial-cell-injury-through-the-jak2-stat3-pathway
#10
Wei-Na Duan, Zhong-Yuan Xia, Min Liu, Qian Sun, Shao-Qing Lei, Xiao-Jing Wu, Qing-Tao Meng, Yan Leng
Previous studies have suggested that the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) pathway is involved in hyperglycemia‑induced lung injury. The present study aimed to investigate the roles of suppressor of cytokine signaling3 (SOCS3) in the regulation of JAK2/STAT3 activation following high glucose (HG) treatment in A549 human pulmonary epithelial cells. Cell viability was evaluated using Cell Counting Kit-8 and lactate dehydrogenase assays. HG‑induced inflammatory injury in A549 cells was assessed through the evaluation of interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) levels using ELISA...
July 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713928/upregulation-of-wnt-signaling-under-hypoxia-promotes-lung-cancer-progression
#11
Chun-Fu Hong, Wei-You Chen, Cheng-Wen Wu
The hypoxic tumor microenvironment induces epithelial-mesenchymal transition (EMT) in tumor cells and increases tumor cell malignancy. Previous studies indicated that malfunction of Wnt signaling is observed in some lung cancer patients. Athough crosstalk between hypoxia and Wnt signaling in tumor cells has recently been revealed, the detailed underlying mechanisms have not been well defined. In the present study, we demonstrated that hypoxia in lung adenocarcinoma cells can enhance Wnt signaling activity by stabilizing β-catenin and altering its localization into the nucleus...
July 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28713784/identification-of-novel-and-efficacious-chemical-compounds-that-disturb-influenza-a-virus-entry-in-vitro
#12
Hany Khalil, Tamer El Malah, Ahmed I Abd El Maksoud, Ibrahim El Halfawy, Ahmed A El Rashedy, Mahmoud El Hefnawy
Influenza A virus is a negative RNA stranded virus of the family Orthomyxoviridae, and represents a major public health threat, compounding existing disease conditions. Influenza A virus replicates rapidly within its host and the segmented nature of its genome facilitates re-assortment, whereby whole genes are exchanged between influenza virus subtypes during replication. Antiviral medications are important pharmacological tools in influenza virus prophylaxis and therapy. However, the use of currently available antiviral is impeded by sometimes high levels of resistance in circulating virus strains...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28712972/arenobufagin-inhibits-prostate-cancer-epithelial-mesenchymal-transition-and-metastasis-by-down-regulating-%C3%AE-catenin
#13
Liping Chen, Weiqian Mai, Minfeng Chen, Jianyang Hu, Zhenjian Zhuo, Xueping Lei, Lijuan Deng, Junshan Liu, Nan Yao, Maohua Huang, Yinghui Peng, Wencai Ye, Dongmei Zhang
Epithelial-mesenchymal transition (EMT) plays an important role in prostate cancer (PCa) metastasis; thus, developing EMT inhibitors may be a feasible treatment for metastatic PCa. Here, we discovered that arenobufagin and four other bufadienolides suppressed PC3 cell EMT. These compounds modulated EMT marker expression with elevating E-cadherin and reducing ZEB1, vimentin and slug expression, and attenuated the migration and invasion of PC3 cells. Among these five compounds, arenobufagin exhibited the most potent activity...
July 13, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28712402/-xinfeng-capsule-improves-lung-function-by-regulating-notch-jagged-hes-axis-of-type-ii-alveolar-epithelial-cells-in-adjuvant-arthritis-rats
#14
Lei Wan, Jian Liu, Chuanbing Huang, Wandong Zhang, Yajun Qi, Xiaojun Zhang
Objective To observe the effect of Xinfeng Capsule (XFC) on Notch/Jagged-HES of type II alveolar epithelial cells (AECII). Methods Rats were divided for four groups: normal control (NC) group, model control (MC) group, leflunomide (LEF) group, XFC group, with 10 rats in each group. Complete Freund's adjuvant (CFA) was injected in the right foot plantar skin of each rat except for the NC group. After adjuvant arthritis was successfully induced, LEF group was given LEF (0.5 mg/100 g), and XFC group was treated with XFC (0...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28711881/clinical-utility-of-ykl-40-in-polymyositis-dermatomyositis-associated-interstitial-lung-disease
#15
Hironao Hozumi, Tomoyuki Fujisawa, Noriyuki Enomoto, Ran Nakashima, Yasunori Enomoto, Yuzo Suzuki, Masato Kono, Masato Karayama, Kazuki Furuhashi, Akihiro Murakami, Naoki Inui, Yutaro Nakamura, Tsuneyo Mimori, Takafumi Suda
OBJECTIVE: Interstitial lung disease (ILD) is involved in polymyositis/dermatomyositis (PM/DM), a disease associated with poor prognoses. Chitinase-3-like-1 protein (YKL-40) has pleiotropic biological activities involved in inflammation, cell proliferation, and tissue remodeling; however, the clinical application of YKL-40 remains limited. We investigated the clinical significance of YKL-40 in PM/DM-ILD. METHODS: Sixty-nine consecutive patients with PM/DM-ILD and 34 healthy controls were analyzed...
July 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/28710803/expression-of-the-major-vault-protein-mvp-and-cellular-vault-particles-in-fish
#16
Alyssa L Margiotta, Lisa J Bain, Charles D Rice
Cellular vaults are ubiquitous 13 mega Da multi-subunit ribonuceloprotein particles that may have a role in nucleocytoplasmic transport. Seventy percent of the vault's mass consists of a ≈100 kDa protein, the major vault protein. In humans, a drug resistance -associated protein, originally identified as lung resistance protein in metastatic lung cancer, was ultimately shown to be the previously described major vault protein. In this study, a partial major vault protein sequence was cloned from channel catfish...
July 15, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28709982/impact-of-zinc-oxide-nanoparticles-on-an-in-vitro-model-of-the-human-air-blood-barrier
#17
Rossella Bengalli, Maurizio Gualtieri, Laura Capasso, Chiara Urani, Marina Camatini
The inhalation of zinc oxide nanoparticles (nZnO) may induce systemic diseases, damages to the alveolar epithelium and inflammatory response to endothelial cells. In this work the use of an in vitro air-blood barrier (ABB) model provided a tool to elucidate the biological mechanisms underlying the potential effects of inhaled nanoparticles (NPs). The ABB model used is composed of a Transwell co-culture of a lung epithelial cell line (NCI-H441) and an immortalized pulmonary microvascular endothelial cell line (HPMEC-ST1...
July 11, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28708889/a-new-index-for-characterizing-micro-bead-motion-in-a-flow-induced-by-ciliary-beating-part-i-experimental-analysis
#18
Mathieu Bottier, Sylvain Blanchon, Gabriel Pelle, Emilie Bequignon, Daniel Isabey, André Coste, Estelle Escudier, James B Grotberg, Jean-François Papon, Marcel Filoche, Bruno Louis
Mucociliary clearance is one of the major lines of defense of the respiratory system. The mucus layer coating the pulmonary airways is moved along and out of the lung by the activity of motile cilia, thus expelling the particles trapped in it. Here we compare ex vivo measurements of a Newtonian flow induced by cilia beating (using micro-beads as tracers) and a mathematical model of this fluid flow, presented in greater detail in a second companion article. Samples of nasal epithelial cells placed in water are recorded by high-speed video-microscopy and ciliary beat pattern is inferred...
July 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28708313/human-biliverdin-reductase-regulates-the-molecular-mechanism-underlying-cancer-development
#19
Min Zhang, Wei Xin, Zhi Yi, Yue Li, Ying Liu, Hongyue Zhang, He Chen, Xinxin Chen, Shujie Tan, Daling Zhu
Human biliverdin reductase (hBVR), is an enzyme, that converts biliverdin to bilirubin, and has been implicated in epithelial-mesenchymal transition?EMT? in breast cancer. However, few studies have investigated the role of hBVR in cell apoptosis in other cancers. Therefore, the aim of this study was to investigate the effects of hBVR in several lines of cancer cells. The results of this study showed that hBVR expression was up-regulated in breast, lung, and liver cancers, but not ovarian cancer. Moreover, hBVR inhibition by small interfering RNA (si-hBVR) attenuated cell survival and increased caspase-3 protein expression, which was mediated by the ERK1/2 signaling pathway in relative cancer cells...
July 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28707078/ctla-4-expression-in-the-non-small-cell-lung-cancer-patient-tumor-microenvironment-diverging-prognostic-impact-in-primary-tumors-and-lymph-node-metastases
#20
Erna-Elise Paulsen, Thomas K Kilvaer, Mehrdad Rakaee, Elin Richardsen, Sigurd M Hald, Sigve Andersen, Lill-Tove Busund, Roy M Bremnes, Tom Donnem
The immune checkpoint receptor CTLA-4 plays a crucial part in negatively regulating T cell activation and maintaining self-tolerance. It is frequently overexpressed in a variety of malignancies, yet its prognostic impact in non-small cell lung cancer (NSCLC) remains unclear. We constructed tissue microarrays from tumor tissue samples and evaluated the immunohistochemical expression of CTLA-4 in 536 patients with primary resected stage I-IIIA NSCLC. Expression of CTLA-4 was analyzed in tumor and stromal primary tumor tissue and in locoregional metastatic lymph nodes...
July 13, 2017: Cancer Immunology, Immunotherapy: CII
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