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https://www.readbyqxmd.com/read/27933701/ccr2-mediates-helicobacter-pylori-induced-immune-tolerance-and-contributes-to-mucosal-homeostasis
#1
Xia Sun, Min Zhang, Mohamad El-Zaatari, Gray B Huffnagle, John Y Kao
BACKGROUND: We previously demonstrated that H. pylori infection leads to increased induction of regulatory T cells in local and systemic immune compartments. Here, we investigate the role of CCR2 in the tolerogenic programing of dendritic cells in a mouse model of H. pylori infection. MATERIALS AND METHODS: CCR2 deficient (CCR2KO) mice and wild-type (Wt) mice infected with H. pylori SS1 strain were analyzed by qPCR and FACS analysis. In vitro, bone marrow-derived DC on day 6 from CCR2KO and Wt mice cocultured with or without H...
December 8, 2016: Helicobacter
https://www.readbyqxmd.com/read/27933651/nuclear-relaxed-elastic-and-piezoelectric-constants-of-materials-computational-aspects-of-two-quantum-mechanical-approaches
#2
Alessandro Erba, Dominique Caglioti, Claudio Marcelo Zicovich-Wilson, Roberto Dovesi
Two alternative approaches for the quantum-mechanical calculation of the nuclear-relaxation term of elastic and piezoelectric tensors of crystalline materials are illustrated and their computational aspects discussed: (i) a numerical approach based on the geometry optimization of atomic positions at strained lattice configurations and (ii) a quasi-analytical approach based on the evaluation of the force- and displacement-response internal-strain tensors as combined with the interatomic force-constant matrix...
December 9, 2016: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/27933528/rna-seq-in-the-collaborative-cross
#3
Richard Green, Courtney Wilkins, Martin T Ferris, Michael Gale
The Collaborative Cross (CC) is a large panel of inbred mouse strains currently being developed for multiple areas of research. Scientists are taking integrated omics-style approaches to collecting data in order to obtain a deeper understanding of the biological mechanisms underlying a number of diverse disease phenotypes. As the cost of the next generation sequencing (NGS) decreases, RNA-sequencing (RNA-seq) has become the favored approach to transcriptomic analyses versus microarrays due to increases in sensitivity and resolution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27933417/on-the-characterization-of-the-heterogeneous-mechanical-response-of-human-brain-tissue
#4
Antonio E Forte, Stephen M Gentleman, Daniele Dini
The mechanical characterization of brain tissue is a complex task that scientists have tried to accomplish for over 50 years. The results in the literature often differ by orders of magnitude because of the lack of a standard testing protocol. Different testing conditions (including humidity, temperature, strain rate), the methodology adopted, and the variety of the species analysed are all potential sources of discrepancies in the measurements. In this work, we present a rigorous experimental investigation on the mechanical properties of human brain, covering both grey and white matter...
December 8, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27933275/an-in-vitro-co-culture-mouse-model-demonstrates-efficient-vaccine-mediated-control-of-francisella-tularensis-schu-s4-and-identifies-nitric-oxide-as-a-predictor-of-efficacy
#5
Igor Golovliov, Helena Lindgren, Kjell Eneslätt, Wayne Conlan, Amandine Mosnier, Thomas Henry, Anders Sjöstedt
Francisella tularensis is a highly virulent intracellular bacterium and cell-mediated immunity is critical for protection, but mechanisms of protection against highly virulent variants, such as the prototypic strain F. tularensis strain SCHU S4, are poorly understood. To this end, we established a co-culture system, based on splenocytes from naïve, or immunized mice and in vitro infected bone marrow-derived macrophages that allowed assessment of mechanisms controlling infection with F. tularensis. We utilized the system to understand why the clpB gene deletion mutant, ΔclpB, of SCHU S4 shows superior efficacy as a vaccine in the mouse model as compared to the existing human vaccine, the live vaccine strain (LVS)...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27933100/genome-and-methylome-of-the-oleaginous-diatom-cyclotella-cryptica-reveal-genetic-flexibility-toward-a-high-lipid-phenotype
#6
Jesse C Traller, Shawn J Cokus, David A Lopez, Olga Gaidarenko, Sarah R Smith, John P McCrow, Sean D Gallaher, Sheila Podell, Michael Thompson, Orna Cook, Marco Morselli, Artur Jaroszewicz, Eric E Allen, Andrew E Allen, Sabeeha S Merchant, Matteo Pellegrini, Mark Hildebrand
BACKGROUND: Improvement in the performance of eukaryotic microalgae for biofuel and bioproduct production is largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptica, which was originally identified in the Aquatic Species Program, is a promising strain of microalgae for large-scale production of biofuel and bioproducts, such as omega-3 fatty acids. RESULTS: We sequenced the nuclear genome and methylome of this oleaginous diatom to identify the genetic traits that enable substantial accumulation of triacylglycerol...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27933059/formation-of-neutrophil-extracellular-traps-under-low-oxygen-level
#7
Katja Branitzki-Heinemann, Helene Möllerherm, Lena Völlger, Diab M Husein, Nicole de Buhr, Stefanie Blodkamp, Friederike Reuner, Graham Brogden, Hassan Y Naim, Maren von Köckritz-Blickwede
Since their discovery, neutrophil extracellular traps (NETs) have been characterized as a fundamental host innate immune defense mechanism. Conversely, excessive NET-release may have a variety of detrimental consequences for the host. A fine balance between NET formation and elimination is necessary to sustain a protective effect during an infectious challenge. Our own recently published data revealed that stabilization of hypoxia-inducible factor 1α (HIF-1α) by the iron chelating HIF-1α-agonist desferoxamine or AKB-4924 enhanced the release of phagocyte extracellular traps...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27933056/characterization-of-three-novel-sxt-r391-integrating-conjugative-elements-icemfuind1a-and-icemfuind1b-and-icemprchn1-identified-in-the-genomes-of-marinomonas-fungiae-jcm-18476-t-and-marinomonas-profundimaris-strain-d104
#8
Jhasketan Badhai, Subrata K Das
The genus Marinomonas comprises Gram negative bacteria which are widespread in the marine environment and there is no report on the genomic analysis of SXT/R391 ICEs derived from this group of bacteria. This study describes the genomic features of three new SXT/R391 integrating conjugating elements (ICEs) identified in the genome of Marinomonas fungiae JCM 18476(T) (ICEMfuInd1a and ICEMfuInd1b) and in Marinomonas profundimaris strain D104 (ICEMprChn1). Structural organizations of the three ICEs were similar to the typical SXT/R391 family of ICEs and showed high degree of conservation in the core genes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27932993/effect-of-permissive-dehydration-on-induction-and-decay-of-heat-acclimation-and-temperate-exercise-performance
#9
Rebecca A Neal, Heather C Massey, Michael J Tipton, John S Young, Jo Corbett
Purpose: It has been suggested that dehydration is an independent stimulus for heat acclimation (HA), possibly through influencing fluid-regulation mechanisms and increasing plasma volume (PV) expansion. There is also some evidence that HA may be ergogenic in temperate conditions and that this may be linked to PV expansion. We investigated: (i) the influence of dehydration on the time-course of acquisition and decay of HA; (ii) whether dehydration augmented any ergogenic benefits in temperate conditions, particularly those related to PV expansion...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27932954/neuronal-release-of-cytokine-il-3-triggered-by-mechanosensitive-autostimulation-of-the-p2x7-receptor-is-neuroprotective
#10
Jason C Lim, Wennan Lu, Jonathan M Beckel, Claire H Mitchell
Mechanical strain due to increased pressure or swelling activates inflammatory responses in many neural systems. As cytokines and chemokine messengers lead to both pro-inflammatory and neuroprotective actions, understanding the signaling patterns triggered by mechanical stress may help improve overall outcomes. While cytokine signaling in neural systems is often associated with glial cells like astrocytes and microglia, the contribution of neurons themselves to the cytokine response is underappreciated and has bearing on any balanced response...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27932671/coupling-of-eit-with-computational-lung-modeling-for-predicting-patient-specific-ventilatory-responses
#11
Christian J Roth, Tobias Becher, Inez Frerichs, Norbert Weiler, Wolfgang A Wall
Providing personalised optimal mechanical ventilation for patients with acute or chronic respiratory failure is still a challenge within a clinical setting for each case anew. In this article, we integrate electrical impedance tomography (EIT) monitoring into a powerful patient-specific computational lung model to create an approach for personalising protective ventilatory treatment. The underlying computational lung model is able to predict global quantities e.g., tracheal flow and tidal volume, as well as local tissue aeration and strains for any ventilation manoeuvre...
December 8, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27932408/early-responses-of-human-periodontal-ligament-fibroblasts-to-cyclic-and-static-mechanical-stretching
#12
Adamantia Papadopoulou, Anna Iliadi, Theodore Eliades, Dimitris Kletsas
OBJECTIVE: To compare the mechanotransduction caused by cyclic and static mechanical strains in human periodontal ligament fibroblasts (hPDLFs) cultured under identical conditions. MATERIALS AND METHODS: hPDLFs, originating from the same donors, were exposed either to cyclic or to static tensile strain using specially designed devices and under identical culture conditions. Activation of all members of mitogen-activated protein kinases (MAPKs) was monitored by western immunoblot analysis...
December 8, 2016: European Journal of Orthodontics
https://www.readbyqxmd.com/read/27932274/a-glutathione-s-transferase-tugstd05-associated-with-acaricide-resistance-in-tetranychus-urticae-directly-metabolizes-the-complex-ii-inhibitor-cyflumetofen
#13
Nena Pavlidi, Mousaalreza Khalighi, Antonis Myridakis, Wannes Dermauw, Nicky Wybouw, Dimitra Tsakireli, Euripides G Stephanou, Nikolaos E Labrou, John Vontas, Thomas Van Leeuwen
Cyflumetofen is a recently introduced acaricide with a novel mode of action, acting as an inhibitor of complex II of mitochondrial electron transport chain. It is activated by hydrolysis and the resulting de-esterified metabolite is a much stronger inhibitor. Cyflumetofen represents a great addition for the control of mite species including Tetranychus urticae, a major agricultural pest, which has the ability to develop resistance to most classes of pesticides rapidly. A resistant strain (Tu008R) was recently described and synergism experiments pointed towards the involvement of GSTs...
December 5, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27931714/host-plant-growth-promotion-and-cadmium-detoxification-in-solanum-nigrum-mediated-by-endophytic-fungi
#14
Abdur Rahim Khan, Ihsan Ullah, Muhammad Waqas, Gun-Seok Park, Abdul Latif Khan, Sung-Jun Hong, Rehman Ullah, Byung Kwon Jung, Chang Eon Park, Shafiq Ur-Rehman, In-Jung Lee, Jae-Ho Shin
Current investigation conducted to evaluate the associated fungal endophyte interactions of a Cd hyper-accumulator Solanum nigrum Korean ecotype under varying concentrations of Cd. Two indole-3-acetic acid (IAA) producing fungal strains, RSF-4L and RSF-6L, isolated from the leaves of S. nigrum, were initially screened for Cd tolerance and accumulation potential. In terms of dry biomass production, the strain RSF-6L showed higher tolerance and accumulation capacity for Cd toxicity in comparison to RSF-4L. Therefore, RSF-6L was applied in vivo to S...
February 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27931684/inhibition-of-apoptosis-by-rv2456c-through-nuclear-factor-%C3%AE%C2%BAb-extends-the-survival-of-mycobacterium-tuberculosis
#15
Kristen L Jurcic Smith, Sunhee Lee
Mycobacterium tuberculosis, the causative agent of tuberculosis, is an intracellular pathogen with several survival mechanisms aimed at subverting the host immune system. Apoptosis has been shown to be mycobactericidal, to activate CD8(+) T cells, and to be modulated by mycobacterial proteins. Since few mycobacterial proteins have so far been directly implicated in the interactions between M. tuberculosis and host cell apoptosis, we screened M. tuberculosis H37Rv transposon mutants to identify mutants that fail to inhibit cell death (FID)...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27931514/vascular-alterations-in-a-murine-model-of-acute-graft-versus-host-disease-are-associated-with-decreased-serum-levels-of-adiponectin-and-an-increased-activity-and-vascular-expression-of-indoleamine-2-3-dioxygenase
#16
Peter M Schmid, Abdellatif Bouazzaoui, Karin Schmid, Christoph M Birner, Christian Schach, Lars S Maier, Ernst Holler, Dierk H Endemann
Graft-versus-host disease (GVHD) is the limiting complication after bone marrow transplantation (BMT), and its pathophysiology seems to be highly influenced by vascular factors. Our study aimed at elucidating possible mechanisms involved in vascular GVHD. For this purpose, we used a fully MHC-mismatched model of BALB/c mice conditioned according to two different intensity protocols with total body irradiation and transplantation of allogeneic (C57BL/6) or syngeneic bone marrow cells and splenocytes. Mesenteric resistance arteries were studied in a pressurized myograph...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27931150/in-vitro-synergism-of-a-water-insoluble-fraction-of-uncaria-tomentosa-combined-with-fluconazole-and-terbinafine-against-resistant-non-candida-albicans-isolates
#17
Renata Cougo Moraes, Anderson Ramos Carvalho, Aline Jacobi Dalla Lana, Samuel Kaiser, Bruna Pippi, Alexandre Meneghello Fuentefria, George González Ortega
CONTEXT: Uncaria tomentosa D.C. (Rubiaceae) has several biological activities, including activity against resistant Candida strains. The synergistic interaction with terbinafine or fluconazole can be an important alternative to overcome this resistance. OBJECTIVES: The potential synergy between a water insoluble fraction (WIF) from Uncaria tomentosa bark and the antifungals terbinafine (TRB) and fluconazole (FLZ) against non-Candida albicans resistant strains was investigated...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27931143/synergistic-in-vitro-activity-of-sodium-houttuyfonate-with-fluconazole-against-clinical-candida-albicans-strains-under-planktonic-growing-conditions
#18
Jing Shao, YanYan Cui, MengXiang Zhang, TianMing Wang, DaQiang Wu, ChangZhong Wang
CONTEXT: Fluconazole resistance is an intractable problem of treating Candida albicans, calling for more antifungal agents to enhance the activity of fluconazole. OBJECTIVE: This work investigates the anti-C. albicans activities of sodium houttuyfonate (SH) and/or fluconazole and the associated mechanism. MATERIALS AND METHODS: The minimum inhibitory concentrations (MICs) of SH and fluconazole both ranging from 0.5 to 1024 μg/mL were determined by broth microdilution method in 19 C...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27930835/effect-of-antibiotics-on-biofilm-inhibition-and-induction-measured-by-real-time-cell-analysis
#19
Maria D Ferrer, Juan C Rodriguez, Ledicia Álvarez, Alejandro Artacho, Gloria Royo, Alex Mira
AIMS: The purpose of the current work was to study the pattern and dynamics of biofilm formation in clinical isolates of Staphylococcus aureus and S. epidermidis in the presence of 10 antibiotics with different action mechanisms. METHODS AND RESULTS: By using impedance measurements in microtiter plates with gold electrodes we have assessed the antibiotic effect on bacterial biofilm growth in real time. The impedance measurements appear to combine both cellular growth and matrix production, representing a measurement of total biofilm mass...
December 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27930725/avertin%C3%A2-but-not-volatile-anesthetics-addressing-the-two-pore-domain-k-channel-task-1-slows-down-cilia-driven-particle-transport-in-the-mouse-trachea
#20
Ghulam Murtaza, Petra Mermer, Uwe Pfeil, Wolfgang Kummer
RATIONALE: Volatile anesthetics inhibit mucociliary clearance in the airways. The two-pore domain K+ channel, TASK-1, represents one of their molecular targets in that they increase its open probability. Here, we determine whether particle transport speed (PTS) at the mucosal surface of the mouse trachea, an important factor of the cilia-driven mechanism in mucociliary clearance, is regulated by TASK-1. METHODOLOGY/RESULTS: RT-PCR analysis revealed expression of TASK-1 mRNA in the manually dissected and laser-assisted microdissected tracheal epithelium of the mouse...
2016: PloS One
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