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https://www.readbyqxmd.com/read/28938198/differentiation-of-black-writing-ink-on-paper-using-luminescence-lifetime-by-time-resolved-luminescence-spectroscopy
#1
Mototsugu Suzuki, Norimitsu Akiba, Kenji Kurosawa, Yoshinori Akao, Yoshiyasu Higashikawa
The time-resolved luminescence spectra and the lifetimes of eighteen black writing inks were measured to differentiate pen ink on altered documents. The spectra and lifetimes depended on the samples. About half of the samples only exhibited short-lived luminescence components on the nanosecond time scale. On the other hand, the other samples exhibited short- and long-lived components on the microsecond time scale. The samples could be classified into fifteen groups based on the luminescence spectra and dynamics...
September 11, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28938164/multifunctional-superparamagnetic-nanoparticles-conjugated-with-fluorescein-labeled-designed-ankyrin-repeat-protein-as-an-efficient-her2-targeted-probe-in-breast-cancer
#2
Dong-Li Li, Jian-Er Tan, Ying Tian, Shun Huang, Peng-Hui Sun, Meng Wang, Yan-Jiang Han, Hong-Sheng Li, Hu-Bing Wu, Xing-Mei Zhang, Yi-Kai Xu, Quan-Shi Wang
Based on the discordance of human epidermal growth factor receptor-2 (HER2) expression between primary and metastatic/recurrent breast cancer, HER2 molecular imaging, which had potential to systemically assess and dynamically monitor HER2 expression, might improve the selection of patients for anti-HER2 therapy. In this study, designed ankyrin repeat protein (DARPin) G3, a novel binding protein with picomolar affinity for HER2, was used and multifunctional superparamagnetic nanoparticles modified with fluorescein-5-maleimide-labeled DARPin G3 (SPIO-G3-5MF) were developed for HER2 imaging...
September 8, 2017: Biomaterials
https://www.readbyqxmd.com/read/28937869/effect-of-microtubule-disruption-on-dynamics-of-acidic-organelles-in-the-axons-of-primary-cultured-retinal-ganglion-cells
#3
Seiji Miyake, Yuji Takihara, Satoshi Yokota, Yoshihiro Takamura, Masaru Inatani
PURPOSE: Axonal transport is fundamental to autophagy in neuronal cells. To understand its biological significance in various conditions, it is necessary to monitor the process of autophagy. However, monitoring methods are often limited to static analyses, such as protein expression and histological observations. Autophagy has multistep process and is highly dynamic; therefore, additional techniques are necessary to study autophagy. In this study, we quantified the dynamics of autophagy-related organelle transport under conditions of dynamic instability and catastrophic disruption of microtubules using in vitro live imaging...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937670/feshbach-resonances-in-the-exit-channel-of-the-f%C3%A2-%C3%A2-ch3oh%C3%A2-%C3%A2-%C3%A2-hf%C3%A2-%C3%A2-ch3o-reaction-observed-using-transition-state-spectroscopy
#4
Marissa L Weichman, Jessalyn A DeVine, Mark C Babin, Jun Li, Lifen Guo, Jianyi Ma, Hua Guo, Daniel M Neumark
The transition state governs how chemical bonds form and cleave during a chemical reaction and its direct characterization is a long-standing challenge in physical chemistry. Transition state spectroscopy experiments based on negative-ion photodetachment provide a direct probe of the vibrational structure and metastable resonances that are characteristic of the reactive surface. Dynamical resonances are extremely sensitive to the topography of the reactive surface and provide an exceptional point of comparison with theory...
October 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28937644/american-sign-language-alphabet-recognition-using-a-neuromorphic-sensor-and-an-artificial-neural-network
#5
Miguel Rivera-Acosta, Susana Ortega-Cisneros, Jorge Rivera, Federico Sandoval-Ibarra
This paper reports the design and analysis of an American Sign Language (ASL) alphabet translation system implemented in hardware using a Field-Programmable Gate Array. The system process consists of three stages, the first being the communication with the neuromorphic camera (also called Dynamic Vision Sensor, DVS) sensor using the Universal Serial Bus protocol. The feature extraction of the events generated by the DVS is the second part of the process, consisting of a presentation of the digital image processing algorithms developed in software, which aim to reduce redundant information and prepare the data for the third stage...
September 22, 2017: Sensors
https://www.readbyqxmd.com/read/28937485/monitoring-dynamic-morphological-changes-with-electrocardiography-gated-dynamic-4-dimensional-computed-tomography-angiography-to-predict-intraoperative-rupture-of-intracranial-aneurysms
#6
Yan Gu, Lun Xu, Chunhong Hu, Meng Luo, Hongwei Zhang, Xiguang Liu
OBJECTIVE: This study aims to evaluate dynamic morphological changes of intracranial aneurysms to predict intraoperative aneurysm rupture (IAR) during clipping. METHODS: Included in this study were 153 patients, who had ruptured and microsurgical-clipped aneurysms. All patients underwent dual-source computed tomography examination of electrocardiography-gated dynamic 4-dimensional computed tomography angiography before clipping. Original scanning data were reconstructed to produce 20 data sets of cardiac cycles with 5% time intervals...
September 20, 2017: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/28937270/feasibility-of-free-breathing-lung-mri-for-radiotherapy-using-non-cartesian-k-space-acquisition-schemes
#7
Shivani Kumar, Robba Rai, Alto Stemmer, Sonal Josan, Lois Holloway, Shalini Vinod, Daniel Moses, Gary Liney
OBJECTIVES: To test a free breathing MRI protocol for anatomical and functional assessment during lung cancer radiotherapy by assessing two non-cartesian acquisition schemes based on a T1W 3D gradient recall echo sequence: (i) stack-of stars (StarVIBE) and (ii) spiral (SpiralVIBE) trajectories Methods: MR images on five healthy volunteers were acquired on a wide bore 3T scanner (Skyra, Siemens Healthcare). Anatomical image quality was assessed on: (1) free breathing (StarVIBE), (2) the standard clinical sequence (VIBE) acquired in a 20 second compliant breath-hold and (3) 20 second non-complaint breath-hold...
September 13, 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/28936656/optimization-of-cell-spreading-and-image-quality-for-the-study-of-chromosomes-in-plant-tissues
#8
Laksana Kantama, Erik Wijnker, Hans de Jong
High-quality chromosome images of mitotic and meiotic cell divisions in plant tissues are inextricably connected with the technical control of cell spread preparations. Superb chromosome slides are the best for studying chromosome morphology and making karyotypes; they also are the best start for a successful fluorescent in situ hybridization experiment. In this study, we describe the essentials for fixation, enzymatic digestion, squash, spread, and dropping protocols for clean and well-differentiated nuclei and chromosome complements...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936654/imaging-ca-2-dynamics-in-wild-type-and-nadph-oxidase-deficient-mutant-pollen-tubes-with-yellow-cameleon-and-confocal-laser-scanning-microscopy
#9
Christina Maria Franck, Jens Westermann, Aurélien Boisson-Dernier
While cytosolic calcium (Ca(2+)) plays a central role in a myriad of signaling pathways as a secondary messenger, how dynamic changes of cytosolic calcium relate to cell growth control remains poorly understood. The engineering and continuous improvements of genetically encoded calcium sensors such as the Yellow Cameleon (YC) sensors combined with advances in microscopy have allowed imaging with great resolution of the spatiotemporal characteristics of cytosolic [Ca(2+)]cyt in individual cells. An exciting new step consists therefore in cautiously studying calcium dynamics in mutant backgrounds that display disturbed cellular growth behavior to further enhance our understanding on growth-related processes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936648/live-cell-imaging-of-f-actin-dynamics-during-fertilization-in-arabidopsis-thaliana
#10
Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima
Fertilization comprises a complex series of cellular processes leading to the fusion of a male and female gamete. Many studies have been carried out to investigate each step of fertilization in plants; however, our comprehensive understanding of all the sequential events during fertilization is still limited. This is largely due to difficulty in investigating events in the female gametophyte, which is deeply embedded in the maternal tissue. Recent advances in confocal microcopy assisted by fluorescent marker lines have contributed to visualizing subcellular dynamics in real time during fertilization in vivo...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936467/peroxisome-motility-measurement-and-quantification-assay
#11
Jeremy Metz, Inês G Castro, Michael Schrader
Organelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ROS homeostasis. They distribute uniformly in mammalian cells and move along microtubules via kinesin and dynein motors. Their metabolic cooperation with mitochondria and the endoplasmic reticulum (ER) is essential for the β-oxidation of fatty acids and the synthesis of myelin lipids and polyunsaturated fatty acids...
September 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28935858/modular-fluorescence-complementation-sensors-for-live-cell-detection-of-epigenetic-signals-at-endogenous-genomic-sites
#12
Cristiana Lungu, Sabine Pinter, Julian Broche, Philipp Rathert, Albert Jeltsch
Investigation of the fundamental role of epigenetic processes requires methods for the locus-specific detection of epigenetic modifications in living cells. Here, we address this urgent demand by developing four modular fluorescence complementation-based epigenetic biosensors for live-cell microscopy applications. These tools combine engineered DNA-binding proteins with domains recognizing defined epigenetic marks, both fused to non-fluorescent fragments of a fluorescent protein. The presence of the epigenetic mark at the target DNA sequence leads to the reconstitution of a functional fluorophore...
September 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28935857/sensorimotor-computation-underlying-phototaxis-in-zebrafish
#13
Sébastien Wolf, Alexis M Dubreuil, Tommaso Bertoni, Urs Lucas Böhm, Volker Bormuth, Raphaël Candelier, Sophia Karpenko, David G C Hildebrand, Isaac H Bianco, Rémi Monasson, Georges Debrégeas
Animals continuously gather sensory cues to move towards favourable environments. Efficient goal-directed navigation requires sensory perception and motor commands to be intertwined in a feedback loop, yet the neural substrate underlying this sensorimotor task in the vertebrate brain remains elusive. Here, we combine virtual-reality behavioural assays, volumetric calcium imaging, optogenetic stimulation and circuit modelling to reveal the neural mechanisms through which a zebrafish performs phototaxis, i.e...
September 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28935459/clinical-and-pathologic-features-of-clinically-occult-synchronous-bilateral-breast-cancers
#14
Lumarie Santiago, Gary Whitman, Cuiyan Wang, Basak E Dogan
PURPOSE: To investigate clinicopathologic breast cancer characteristics associated with the identification of synchronous bilateral breast cancer (SBBC) on dynamic contrast-enhanced breast magnetic resonance imaging. METHODS: We performed a retrospective review of 311 consecutive patients newly diagnosed with breast cancer who underwent diagnostic mammography, ultrasonography, and dynamic contrast-enhanced breast magnetic resonance imaging (DCE-MRI) at our institution...
August 24, 2017: Current Problems in Diagnostic Radiology
https://www.readbyqxmd.com/read/28935166/mitochondrial-nanotunnels
#15
REVIEW
Amy E Vincent, Doug M Turnbull, Veronica Eisner, György Hajnóczky, Martin Picard
Insight into the regulation of complex physiological systems emerges from understanding how biological units communicate with each other. Recent findings show that mitochondria communicate at a distance with each other via nanotunnels, thin double-membrane protrusions that connect the matrices of non-adjacent mitochondria. Emerging evidence suggest that mitochondrial nanotunnels are generated by immobilized mitochondria and transport proteins. This review integrates data from the evolutionarily conserved structure and function of intercellular projections in bacteria with recent developments in mitochondrial imaging that permit nanotunnel visualization in eukaryotes...
September 18, 2017: Trends in Cell Biology
https://www.readbyqxmd.com/read/28934696/synthesis-and-application-of-a-plastic-antibody-in-electrochemical-microfluidic-platform-for-oxytocin-determination
#16
Piyush Sindhu Sharma, Zofia Iskierko, Krzysztof Noworyta, Maciej Cieplak, Pawel Borowicz, Wojciech Lisowski, Francis D'Souza, Wlodzimierz Kutner
By means of molecular imprinting of a conducting polymer, molecular cavities selective for oxytocin nonapeptide, an autism biomarker, were designed. Embedding of the oxytocin template, and then its extracting from the molecularly imprinted polymer (MIP) was confirmed by the XPS analysis. AFM imaging of the MIP film surface indicated changes in mechanical properties of the film after template extraction. The MIP synthetic receptor was deposited by potentiodynamic electropolymerization as a thin film on an Au film electrode in an electrochemical miniaturized microfluidic cell...
September 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28934354/synthesis-of-irregular-graphene-oxide-tubes-using-green-chemistry-and-their-potential-use-as-reinforcement-materials-for-biomedical-applications
#17
Ángel Serrano-Aroca, Sanjukta Deb
Micrometer length tubes of graphene oxide (GO) with irregular form were synthesised following facile and green metal complexation reactions. These materials were obtained by crosslinking of GO with calcium, zinc or strontium chlorides at three different temperatures (24, 34 and 55°C) using distilled water as solvent for the compounds and following a remarkably simple and low-cost synthetic method, which employs no hazardous substances and is conducted without consumption of thermal or sonic energy. These irregular continuous GO networks showed a very particular interconnected structure by Field Emission Scanning Electron Microscopy with Energy-Disperse X-Ray Spectroscopy for elemental analysis and High-resolution Transmission Electron Microscopy with Scanning Transmission Electron Microscope Dark Field Imaging, and were analysed by Raman Spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28934283/grading-of-invasive-breast-carcinoma-through-grassmannian-vlad-encoding
#18
Kosmas Dimitropoulos, Panagiotis Barmpoutis, Christina Zioga, Athanasios Kamas, Kalliopi Patsiaoura, Nikos Grammalidis
In this paper we address the problem of automated grading of invasive breast carcinoma through the encoding of histological images as VLAD (Vector of Locally Aggregated Descriptors) representations on the Grassmann manifold. The proposed method considers each image as a set of multidimensional spatially-evolving signals that can be efficiently modeled through a higher-order linear dynamical systems analysis. Subsequently, each H&E (Hematoxylin and Eosin) stained breast cancer histological image is represented as a cloud of points on the Grassmann manifold, while a vector representation approach is applied aiming to aggregate the Grassmannian points based on a locality criterion on the manifold...
2017: PloS One
https://www.readbyqxmd.com/read/28934279/spontaneous-non-canonical-assembly-of-ccmk-hexameric-components-from-%C3%AE-carboxysome-shells-of-cyanobacteria
#19
Luis F Garcia-Alles, Eric Lesniewska, Katharina Root, Nathalie Aubry, Nicolas Pocholle, Carlos I Mendoza, Eric Bourillot, Konstantin Barylyuk, Denis Pompon, Renato Zenobi, David Reguera, Gilles Truan
CcmK proteins are major constituents of icosahedral shells of β-carboxysomes, a bacterial microcompartment that plays a key role for CO2 fixation in nature. Supported by the characterization of bidimensional (2D) layers of packed CcmK hexamers in crystal and electron microscopy structures, CcmK are assumed to be the major components of icosahedral flat facets. Here, we reassessed the validity of this model by studying CcmK isoforms from Synechocystis sp. PCC6803. Native mass spectrometry studies confirmed that CcmK are hexamers in solution...
2017: PloS One
https://www.readbyqxmd.com/read/28934254/deep-reconstruction-model-for-dynamic-pet-images
#20
Jianan Cui, Xin Liu, Yile Wang, Huafeng Liu
Accurate and robust tomographic reconstruction from dynamic positron emission tomography (PET) acquired data is a difficult problem. Conventional methods, such as the maximum likelihood expectation maximization (MLEM) algorithm for reconstructing the activity distribution-based on individual frames, may lead to inaccurate results due to the checkerboard effect and limitation of photon counts. In this paper, we propose a stacked sparse auto-encoder based reconstruction framework for dynamic PET imaging. The dynamic reconstruction problem is formulated in a deep learning representation, where the encoding layers extract the prototype features, such as edges, so that, in the decoding layers, the reconstructed results are obtained through a combination of those features...
2017: PloS One
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