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https://www.readbyqxmd.com/read/28819625/antagonistic-properties-of-some-halophilic-thermoactinomycetes-isolated-from-superficial-sediment-of-a-solar-saltern-and-production-of-cyclic-antimicrobial-peptides-by-the-novel-isolate-paludifilum-halophilum
#1
Donyez Frikha Dammak, Ziad Zarai, Soumaya Najah, Rayed Abdennabi, Lassaad Belbahri, Mostafa E Rateb, Hafedh Mejdoub, Sami Maalej
This study has focused on the isolation of twenty-three halophilic actinomycetes from two ponds of different salinity and the evaluation of their ability to exert an antimicrobial activity against both their competitors and several other pathogens. From the 23 isolates, 18 strains showed antagonistic activity, while 19 showed activities against one or more of the seven pathogen strains tested. Six strains exhibited consistent antibacterial activity against Gram-negative and Gram-positive pathogens characterized at the physiological and molecular levels...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28811543/4-e-p-tolylimino-methylbenzene-1-2-diol-timbd-suppresses-hiv1-gp120-mediated-production-of-il6-and-il8-but-not-ccl5
#2
Fatma Abdalla, Anantha Nookala, Subhash B Padhye, Anil Kumar, Hari K Bhat
Human immunodeficiency virus (HIV) has been associated with inflammatory effects that may potentially result in neurodegenerative changes and a number of newer chemotherapeutic agents are being tested to ameliorate these effects. In this study, we investigated the anti-neuroinflammatory activity of a novel resveratrol analog 4-(E)-{(p-tolylimino)-methylbenzene-1,2-diol} (TIMBD) against HIV1-gp120 induced neuroinflammation in SVG astrocytes. SVG astrocytic cells were pretreated with TIMBD or resveratrol (RES) and then transfected with a plasmid encoding HIV1-gp120...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28808378/rutamarin-an-active-constituent-from-ruta-angustifolia-pers-induced-apoptotic-cell-death-in-the-ht29-colon-adenocarcinoma-cell-line
#3
Shafinah Ahmad Suhaimi, Sok Lai Hong, Sri Nurestri Abdul Malek
BACKGROUND: Ruta angustifolia Pers. is a perennial herb that is cultivated worldwide, including Southeast Asia, for the treatment of various diseases as traditional medicine. OBJECTIVE: The purpose of the study was to identify an active principle of R. angustifolia and to investigate its effect on the HT29 cell death. MATERIALS AND METHODS: The methanol and fractionated extracts (hexane, chloroform, ethyl acetate, and water) of R. angustifolia Pers...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28805973/experimental-and-clinical-evidence-of-differential-effects-of-magnesium-sulfate-on-neuroprotection-and-angiogenesis-in-the-fetal-brain
#4
Matthieu Lecuyer, Marina Rubio, Clément Chollat, Maryline Lecointre, Sylvie Jégou, Philippe Leroux, Carine Cleren, Isabelle Leroux-Nicollet, Loic Marpeau, Denis Vivien, Stéphane Marret, Bruno J Gonzalez
Clinical studies showed beneficial effects of magnesium sulfate regarding the risk of cerebral palsy. However, regimen protocols fluctuate worldwide and risks of adverse effects impacting the vascular system have been reported for human neonates, keeping open the question of the optimal dosing. Using clinically relevant concentrations and doses of magnesium sulfate, experiments consisted of characterizing, respectively, ex vivo and in vivo, the effects of magnesium sulfate on the nervous and vascular systems of mouse neonates by targeting neuroprotection, angiogenesis, and hemodynamic factors and in measuring, in human fetuses, the impact of a 4-g neuroprotective loading dose of magnesium sulfate on brain hemodynamic parameters...
August 2017: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/28802255/simultaneous-targeting-of-two-distinct-epitopes-on-met-effectively-inhibits-met-and-hgf-driven-tumor-growth-by-multiple-mechanisms
#5
Michael M Grandal, Serhiy Havrylov, Thomas Tuxen Poulsen, Klaus Koefoed, Anna Dahlman, Gunther R Galler, Paolo Conrotto, Sara Collins, Karsten W Eriksen, Dafna Kaufman, George F Vande Woude, Helle J Jacobsen, Ivan D Horak, Michael Kragh, Johan Lantto, Thomas Bouquin, Morag Park, Mikkel Wandahl Pedersen
Increased MET activity is linked with poor prognosis and outcome in several human cancers currently lacking targeted therapies. Here we report on the characterization of Sym015, an antibody mixture composed of two humanized IgG1 antibodies against non-overlapping epitopes of MET. Sym015 was selected by high-throughput screening searching for antibody mixtures with superior growth inhibitory activity against MET-dependent cell lines. Synergistic inhibitory activity of the antibodies comprising Sym015 was observed in several cancer cell lines harboring amplified MET locus and was confirmed in vivo Sym015 was found to exert its activity via multiple mechanisms...
August 11, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28797724/the-effect-of-newly-synthesized-progesterone-derivatives-on-apoptotic-and-angiogenic-pathway-in-mcf-7-breast-cancer-cells
#6
Shaymaa M M Yahya, Abdou O Abdelhamid, Mervat M Abd-Elhalim, Ghada H Elsayed, Emad F Eskander
Due to its high potency and selectivity, anticancer agents consisting of combined molecules have gained great interests. The current study introduces newly synthesized progesterone derivatives of promising anticancer effect. Moreover, the pro-apoptotic and anti-angiogenic effects of these compounds were studied extensively. Several thiazole, pyridine, pyrazole, thiazolopyridine and pyrazolopyridine progesterone derivatives were synthesized. The structure of the novel progesterone derivatives was elucidated and confirmed using the analytical and spectral data...
August 8, 2017: Steroids
https://www.readbyqxmd.com/read/28791908/synthesis-and-biological-evaluation-of-novel-7-hydroxy-4-phenylchromen-2-one-linked-to-triazole-moieties-as-potent-cytotoxic-agents
#7
Chuan-Feng Liu, Qing-Kun Shen, Jia-Jun Li, Yu-Shun Tian, Zheshan Quan
A new series of novel 7-hydroxy-4-phenylchromen-2-one (1a)-linked 1,2,4-triazoles were synthesised using a click chemistry approach. All derivatives were subjected to 3-(4,5-dimethylthiazol-yl)-diphenyl tetrazolium bromide (MTT) cytotoxicity screening against a panel of six different human cancer cell lines (AGS, MGC-803, HCT-116, A-549, HepG2, and HeLa) to assess their cytotoxic potential. Among the tested molecules, some of the analogues showed better cytotoxic activity than that shown by the 7-hydroxy-4-phenylchromen-2-one (1a)...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28790851/identification-of-the-anticancer-effects-of-a-novel-proteasome-inhibitor-ixazomib-on-colorectal-cancer-using-a-combined-method-of-microarray-and-bioinformatics-analysis
#8
Qiaowei Fan, Bingrong Liu
PURPOSE: The study aimed to explore the anticancer effects of a novel proteasome inhibitor, ixazomib, on colorectal cancer (CRC) using a combined method of microarray and bioinformatics analysis. MATERIALS AND METHODS: Cell proliferation was tested by Cell Counting Kit-8 (CCK-8) assay for SW620 cells treated with different concentrations of ixazomib and different treatment times. The microarray analysis was conducted for six samples, including three samples of SW620 cells untreated with ixazomib and three samples of SW620 cells treated with ixazomib...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28789971/the-use-of-high-throughput-screening-techniques-to-evaluate-mitochondrial-toxicity
#9
Lauren P Wills
Toxicologists and chemical regulators depend on accurate and effective methods to evaluate and predict the toxicity of thousands of current and future compounds. Robust high-throughput screening (HTS) experiments have the potential to efficiently test large numbers of chemical compounds for effects on biological pathways. HTS assays can be utilized to examine chemical toxicity across multiple mechanisms of action, experimental models, concentrations, and lengths of exposure. Many agricultural, industrial, and pharmaceutical chemicals classified as harmful to human and environmental health exert their effects through the mechanism of mitochondrial toxicity...
August 5, 2017: Toxicology
https://www.readbyqxmd.com/read/28776672/mechanisms-involved-in-the-cytotoxic-action-of-brazilian-propolis-and-caffeic-acid-against-hep-2-cells-and-modulation-of-p-glycoprotein-activity
#10
Lívia M da Silva, Yahima Frión-Herrera, Ariane R Bartolomeu, Carolina Mendonça Gorgulho, José M Sforcin
OBJECTIVES: The effects of propolis and phenolic compounds (caffeic acid - Caf; dihydrocinnamic acid - Cin; p-coumaric acid - Cou) in the same quantity found in our propolis sample were investigated on human laryngeal epidermoid carcinoma (HEp-2) cells. METHODS: Cell viability, apoptosis/necrosis and cell cycle arrest, P53 and CASPASE-3 gene expression, generation of reactive oxygen species (ROS) and the ability of propolis to induce doxorubicin (DOX) efflux using a P-glycoprotein (P-gp) inhibitor (verapamil) were assayed...
August 4, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28769562/mithramycin-loaded-mpeg-plga-nanoparticles-exert-potent-antitumor-efficacy-against-pancreatic-carcinoma
#11
Xu-Jie Liu, Liang Li, Xiu-Jun Liu, Yi Li, Chun-Yan Zhao, Rui-Qi Wang, Yong-Su Zhen
Previous studies have shown that mithramycin A (MIT) is a promising candidate for the treatment of pancreatic carcinoma through inhibiting transcription factor Sp1. However, systemic toxicities may limit its clinical application. Here, we report a rationally designed formulation of MIT-loaded nanoparticles (MIT-NPs) with a small size and sustained release for improved passive targeting and enhanced therapeutic efficacy. Nearly spherical MIT-NPs with a mean particle size of 25.0±4.6 nm were prepared by encapsulating MIT into methoxy poly(ethylene glycol)-block-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (NPs) with drug loading of 2...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28767592/bmp4-rs17563-polymorphism-and-nonsyndromic-cleft-lip-with-or-without-cleft-palate-a-meta-analysis
#12
Yue-Hua Li, Jiaomei Yang, Ju-Lei Zhang, Jia-Qi Liu, Zhao Zheng, Da-Hai Hu
BACKGROUND: Previous studies have investigated the relationship between human bone morphogenetic protein 4 gene (BMP4) rs17563 polymorphism and nonsyndromic cleft lip with or without cleft palate (NSCL/P). However, the results remained inconsistent. Therefore, we conducted a meta-analysis to assess the effect of BMP4 rs17563 polymorphism on NSCL/P. METHODS: Electronic searches in 5 databases were conducted to select all eligible studies up to March 2017. Odds ratios (ORs) with the corresponding 95% confidence intervals (CIs) were calculated to estimate the association...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28765934/runx3-plays-a-tumor-suppressor-role-by-inhibiting-cell-migration-invasion-and-angiogenesis-in-oral-squamous-cell-carcinoma
#13
Wei-Na Zhou, Yi-Fei Du, Jin Bai, Xiao-Meng Song, Yang Zheng, Hua Yuan, Wei Zhang, Zheng-Dong Zhang, Yu-Nong Wu
Although aberrant expression of Runt-related transcription factor 3 (RUNX3) contributes to tumor progression and metastasis in a number of carcinomas, the status of RUNX3 and its correlation with prognosis in oral squamous cell carcinomas (OSCC) are still controversial. The aim of present study was to investigate the function of RUNX3 in OSCC and the underlying molecular mechanisms. Tissue microarray (TMA) consisting of 232 OSCC specimens was used to detect the expression of RUNX3 by immunohistochemistry method...
July 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28763747/long-intergenic-noncoding-rna-00305-sponges-mir-136-to-regulate-the-hypoxia-induced-apoptosis-of-vascular-endothelial-cells
#14
Bo-Ya Zhang, Zhe Jin, Zhuo Zhao
BACKGROUND: Apoptosis in vascular endothelial cells (VECs) are closely correlated to multiple endotheliocyte-related cardiovascular diseases, for example atherosclerosis. Long non-coding RNAs (lncRNAs) have been testified to play important role in regulation of VECs. The purpose of this study is to investigate the potential regulation of lncRNA long intergenic noncoding RNA 00305 (LINC00305) on hypoxia induced VECs. METHODS: Human umbilical vein endothelial cells (HUVECs) were respectively induced with normoxia or hypoxia (1%)...
July 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28758133/probiotic-lactobacillus-strains-stimulate-the-inflammatory-response-and-activate-human-macrophages
#15
L M Rocha-Ramírez, R A Pérez-Solano, S L Castañón-Alonso, S S Moreno Guerrero, A Ramírez Pacheco, M García Garibay, C Eslava
Lactobacilli have been shown to promote health functions. In this study, we analyzed the mechanism by which four different strains of probiotics affected innate immunity, such as regulation of ROS, cytokines, phagocytosis, bactericidal activity, signaling by NF-κB pp65, and TLR2 activation. The production of ROS was dependent on the concentration and species of Lactobacillus. The results obtained from the tested strains (Lactobacillus rhamnosus GG, L. rhamnosus KLSD, L. helveticus IMAU70129, and L. casei IMAU60214) showed that strains induced early proinflammatory cytokines such as IL-8,TNF-α, IL-12p70, and IL-6...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28756292/anticancer-potential-of-new-steroidal-thiazolidin-4-one-derivatives-mechanisms-of-cytotoxic-action-and-effects-on-angiogenesis-in-vitro
#16
Marijana B Živković, Ivana Z Matić, Marko V Rodić, Irena T Novaković, Ana M Krivokuća, Dušan M Sladić, Natalija M Krstić
The synthesis and cytotoxic activities determination of new steroidal mono- and bis(thiazolidin-4-ones) 4a-f and 5a-f have been performed. Their anticancer action was also evaluated in comparison to previously synthesized and reported corresponding steroidal thiosemicarbazones. All compounds were obtained as stereoisomeric mixtures with different configuration (E or Z) in the hydrazone moiety at the C-3 position. After several consecutive crystallizations diastereomerically pure major (E)-isomers of mono-thiazolidin-4-ones were isolated...
July 26, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28756149/anticancer-potential-of-amaryllidaceae-alkaloids-evaluated-by-screening-with-a-panel-of-human-cells-real-time-cellular-analysis-and-ehrlich-tumor-bearing-mice
#17
Radim Havelek, Darina Muthna, Pavel Tomsik, Karel Kralovec, Martina Seifrtova, Lucie Cahlikova, Anna Hostalkova, Marcela Safratova, Maria Perwein, Eva Cermakova, Martina Rezacova
In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model...
July 26, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28753929/whole-grain-intake-and-glycaemic-control-in-healthy-subjects-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#18
REVIEW
Stefano Marventano, Claudia Vetrani, Marilena Vitale, Justyna Godos, Gabriele Riccardi, Giuseppe Grosso
BACKGROUNDS: There is growing evidence from both observational and intervention studies that Whole Grain (WG) cereals exert beneficial effects on human health, especially on the metabolic profile. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCT) to assess the acute and medium/long-term effect of WG foods on glycaemic control and insulin sensitivity in healthy individuals. METHODS: A search for all the published RCT on the effect of WG food intake on glycaemic and insulin response was performed up to December 2016...
July 19, 2017: Nutrients
https://www.readbyqxmd.com/read/28750654/different-therapeutic-effects-of-cells-derived-from-human-amniotic-membrane-on-premature-ovarian-aging-depend-on-distinct-cellular-biological-characteristics
#19
Chenyue Ding, Hong Li, Yun Wang, Fuxin Wang, Huihua Wu, Rulei Chen, Jinghuan Lv, Wei Wang, Boxian Huang
BACKGROUND: Many reports have shown that various kinds of stem cells have the ability to recover premature ovarian aging (POA) function. Transplantation of human amniotic epithelial cells (hAECs) improves ovarian function damaged by chemotherapy in a mice model. Understanding of how to evaluate the distinct effects of adult stem cells in curing POA and how to choose stem cells in clinical application is lacking. METHODS: To build a different degrees of POA model, mice were administered different doses of cyclophosphamide: light dose (70 mg/kg, 2 weeks), medium dose (70 mg/kg, 1 week; 120 mg/kg, 1 week), and high dose (120 mg/kg, 2 weeks)...
July 27, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28750064/modified-human-glucagon-like-peptide-1-glp-1-produced-in-e-coli-has-a-long-acting-therapeutic-effect-in-type-2-diabetic-mice
#20
Fangfang Xu, Kevin Yueju Wang, Nan Wang, Gangqiang Li, Dehu Liu
Glucagon-like peptide 1 (GLP-1) is a very potent insulinotropic hormone secreted into the blood stream after eating. Thus, it has potential to be used in therapeutic treatment of diabetes. The half-life of GLP-1, however, is very short due to its rapid cleavage by dipeptidyl peptidase IV (DPP-IV). This presents a great challenge if it is to be used as a therapeutic drug. GLP-1, like many other small peptides, is commonly produced through chemical synthesis, but is limited by cost and product quantity. In order to overcome these problems, a sequence encoding a six codon-optimized tandem repeats of modified GLP-1 was constructed and expressed in the E...
2017: PloS One
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