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https://www.readbyqxmd.com/read/28535507/cardiac-cell-therapies-for-the-treatment-of-acute-myocardial-infarction-a-meta-analysis-from-mouse-studies
#1
Cajetan Immanuel Lang, Markus Wolfien, Anne Langenbach, Paula Müller, Olaf Wolkenhauer, Arash Yavari, Hüseyin Ince, Gustav Steinhoff, Bernd Joachim Krause, Robert David, Änne Glass
AIMS: Stem cell-based regenerative therapies for the treatment of ischemic myocardium are currently a subject of intensive investigation. A variety of cell populations have been demonstrated to be safe and to exert some positive effects in human Phase I and II clinical trials, however conclusive evidence of efficacy is still lacking. While the relevance of animal models for appropriate pre-clinical safety and efficacy testing with regard to application in Phase III studies continues to increase, concerns have been expressed regarding the validity of the mouse model to predict clinical results...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28533178/mucin-and-carbohydrate-stimulated-adhesion-and-subproteome-changes-of-the-probiotic-bacterium-lactobacillus-acidophilus-ncfm
#2
Hasan Ufuk Celebioglu, Sita Vaag Olesen, Kennie Prehn, Sampo J Lahtinen, Susanne Brix, Maher Abou Hachem, Birte Svensson
Adhesion to intestinal mucosa is a crucial property for probiotic bacteria. Adhesion is thought to increase host-bacterial interactions, thus potentially enabling health benefits to the host. Molecular events connected with adhesion and surface proteome changes were investigated for the probiotic Lactobacillus acidophilus NCFM cultured with established or emerging prebiotic carbohydrates as carbon source and in the presence of mucin, the glycoprotein of the epithelial mucus layer. Variation in adhesion to HT29-cells and mucin was associated with carbon source and mucin-induced subproteome abundancy differences...
May 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28533127/primary-human-polarized-small-intestinal-epithelial-barriers-respond-differently-to-a-hazardous-and-an-innocuous-protein
#3
A D Eaton, C Zimmermann, B Delaney, B P Hurley
An experimental platform employing human derived intestinal epithelial cell (IEC) line monolayers grown on permeable Transwell(®) filters was previously investigated to differentiate between hazardous and innocuous proteins. This approach was effective at distinguishing these types of proteins and perturbation of monolayer integrity, particularly transepithelial electrical resistance (TEER), was the most sensitive indicator. In the current report, in vitro indicators of monolayer integrity, cytotoxicity, and inflammation were evaluated using primary (non-transformed) human polarized small intestinal epithelial barriers cultured on Transwell(®) filters to compare effects of a hazardous protein (Clostridium difficile Toxin A [ToxA]) and an innocuous protein (bovine serum albumin [BSA])...
May 19, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28528091/antiproliferative-activity-of-ontario-grown-onions-against-colorectal-adenocarcinoma-cells
#4
Abdulmonem I Murayyan, Cynthya M Manohar, Gordon Hayward, Suresh Neethirajan
Cancer is the leading cause of mortality in Canada and other industrialized nations; the development of new/improved cancer therapies is desperately needed and continues to be a major focus of cancer research. Flavonoids, which are found in high levels in onions, have been shown to exert antiproliferative and potentially anti-cancer activities. To test their therapeutic potential, we assessed the antiproliferative, cytotoxic, apoptosis-inducing, and anti-migratory activities of five onion varieties grown in Ontario against human adenocarcinoma (Caco-2) cells...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28527708/cortical-cytoskeleton-dynamics-regulates-plasma-membrane-calcium-atpase-isoform-2-pmca2-activity
#5
Marianela G Dalghi, Mariela Ferreira-Gomes, Nicolás Montalbetti, Alexandre Simonin, Emanuel E Strehler, Matthias A Hediger, Juan Pablo Rossi
We have previously shown that purified actin can directly bind to human plasma membrane Ca(2+) ATPase 4b (hPMCA4b) and exert a dual modulation on its Ca(2+)-ATPase activity: F-actin inhibits PMCA while short actin oligomers may contribute to PMCA activation. These studies had to be performed with purified proteins given the nature of the biophysical and biochemical approaches used. To assess whether a functional interaction between the PMCAs and the cortical cytoskeleton is of physiological relevance, we characterized this phenomenon in the context of a living cell by monitoring in real-time the changes in the cytosolic calcium levels ([Ca(2+)]CYT)...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28525845/novel-amidino-substituted-benzimidazole-and-benzothiazole-benzo-b-thieno-2-carboxamides-exert-strong-antiproliferative-and-dna-binding-properties
#6
Maja Cindrić, Samy Jambon, Anja Harej, Sabine Depauw, Marie-Hélène David-Cordonnier, Sandra Kraljević Pavelić, Grace Karminski-Zamola, Marijana Hranjec
Within this manuscript design, synthesis of novel 2-imidazolinyl substituted benzo[b]thieno-2-carboxamides bearing either benzimidazole or benzothiazole subunit and biological activity are presented and described. The antiproliferative activities were assessed in vitro on a panel of human cancer cell lines. Tested compounds showed moderate activity while cytotoxicity on normal fibroblasts was lower in comparison with 5-fluorouracile. The variations of 2-imidazolinyl substituent at heteroaromatic subunits in different positions led to different cytotoxic properties...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28509786/igg4-related-disease-of-the-biliary-tract-and-pancreas-clinical-and-experimental-advances
#7
Lowiek M Hubers, Ulrich Beuers
PURPOSE OF REVIEW: IgG4-related disease (IgG4-RD) is an immune-mediated disease of unknown cause. It predominantly affects the biliary tract [IgG4-associated cholangitis (IAC)] and pancreas [autoimmune pancreatitis (AIP)] of mostly elderly men. Accurate diagnostic tests are lacking. Patients benefit from predniso(lo)ne treatment. However, disease relapse is often seen. This review will address pathophysiological aspects and advances in diagnostic and therapeutic strategies. RECENT FINDINGS: The role of IgG1 and IgG4 in the pathophysiology of IgG4-RD was studied in mice which showed more intense organ damage of pancreas and salivary glands when IgG1 rather than IgG4 of patients with IgG4-RD was injected...
May 13, 2017: Current Opinion in Gastroenterology
https://www.readbyqxmd.com/read/28501767/garlic-derived-organosulfur-compound-exerts-antitumor-efficacy-via-activation-of-mapk-pathway-and-modulation-of-cytokines-in-sgc-7901-tumor-bearing-mice
#8
Xiaoyan Jiang, Xiaosong Zhu, Weizhen Huang, Hongya Xu, Zhongxi Zhao, Siying Li, Shanzhong Li, Jianhua Cai, Jimin Cao
Diallyl trisulfide (DATS), a natural agent derived from garlic, has been tested for its antigastric cancer activities in various preliminary studies. However, more systematic pharmacodymatic (PD) and mechanistic evaluations are clearly needed. The aim of this study was to investigate the antitumor effects of DATS in the treatment of human gastric cancer cell SGC-7901 both in vitro and in vivo using widely recommended study procedures. DATS suppressed cancer cells proliferation and induced cell cycle arrest accompanied by an increase in the expressions of cyclin A2 and cyclin B1 in SGC-7901 cancer cells...
May 11, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28497228/18-des-hydroxy-cytochalasin-an-antiparasitic-compound-of-diaporthe-phaseolorum-92c-an-endophytic-fungus-isolated-from-combretum-lanceolatum-pohl-ex-eichler
#9
Elson Rudimar Brissow, Igor Pereira da Silva, Kátia Aparecida de Siqueira, Jaqueline Alves Senabio, Leticia Pereira Pimenta, Ana Helena Januário, Lizandra Guidi Magalhães, Ricardo Andrade Furtado, Denise Crispim Tavares, Policarpo Ademar Sales Junior, Jane Lima Santos, Marcos Antônio Soares
Chemical investigation of the ethyl acetate extract from the endophytic fungus Diaporthe phaseolorum-92C (92C) isolated from the roots of Combretum lanceolatum led to the isolation of 18-des-hydroxy Cytochalasin H (compound 1). The trypanocidal and schistosomicidal activity and cytotoxicity of the extract from 92C were evaluated. The schistosomicidal, leishmanicidal, antimicrobial, and antioxidant actions, as well as the antitumor activity against the breast cancer cells MDA-MB-231 and MCF-7, and the cytotoxicity towards normal human lung fibroblasts GM07492A of compound 1 was tested...
May 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28487282/regulation-of-spermatogenesis-by-a-local-functional-axis-in-the-testis-role-of-the-basement-membrane-derived-noncollagenous-1-domain-peptide
#10
Haiqi Chen, Dolores D Mruk, Will M Lee, C Yan Cheng
Spermatogenesis takes place in the epithelium of the seminiferous tubules of the testes, producing millions of spermatozoa per day in an adult male in rodents and humans. Thus, multiple cellular events that are regulated by an array of signaling molecules and pathways are tightly coordinated to support spermatogenesis. Here, we report findings of a local regulatory axis between the basement membrane (BM), the blood-testis barrier (BTB), and the apical ectoplasmic specialization (apical ES; a testis-specific, actin-rich adherens junction at the Sertoli cell-spermatid interface) to coordinate cellular events across the seminiferous epithelium during the epithelial cycle...
May 9, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28482892/a-preliminary-investigation-into-the-design-of-pressure-cushions-and-their-potential-applications-for-forearm-robotic-orthoses
#11
N Alavi, S Zampierin, M Komeili, S Cocuzza, S Debei, C Menon
BACKGROUND: Load cells are often used in rehabilitation robotics to monitor human-robot interaction. While load cells are accurate and suitable for the stationary end-point robots used in rehabilitation hospitals, their cost and inability to conform to the shape of the body hinder their application in developing affordable and wearable robotic orthoses for assisting individuals in the activities of daily living. This exploratory work investigates the possibility of using an alternative technology, namely compliant polymeric air cushions, to measure interaction forces between the user and a wearable rigid structure...
May 8, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28479226/bifidobacterium-adolescentis-im38-ameliorates-high-fat-diet-induced-colitis-in-mice-by-inhibiting-nf-%C3%AE%C2%BAb-activation-and-lipopolysaccharide-production-by-gut-microbiota
#12
Su-Min Lim, Dong-Hyun Kim
Gut microbiota play essential roles in the regulation of human metabolism via symbiotic interactions with the host. Prolonged consumption of high-fat diet (HFD) elevates the Firmicutes to Bacteroidetes ratio and lipopolysaccharide (LPS) production by gut microbiota, thereby increasing the probability of developing metabolic and immune disorders such as obesity and colitis. The use of probiotics with anti-inflammatory properties has been suggested to counteract this effect. Here, we tested whether Bifidobacterium adolescentis IM38, which inhibited nuclear factor-kappa B (NF-κB) activation in Caco-2 cells and peritoneal macrophages and inhibited Escherichia coli LPS production, exerted an anticolitic effect in mice with HFD-induced obesity...
April 19, 2017: Nutrition Research
https://www.readbyqxmd.com/read/28476158/melicope-ptelefolia-leaf-extracts-exhibit-antioxidant-activity-and-exert-anti-proliferative-effect-with-apoptosis-induction-on-four-different-cancer-cell-lines
#13
Mohammad Faujul Kabir, Johari Mohd Ali, Mitra Abolmaesoomi, Onn Haji Hashim
BACKGROUND: Melicope ptelefolia is a well-known herb in a number of Asian countries. It is often used as vegetable salad and traditional medicine to address various ailments. However, not many studies have been currently done to evaluate the medicinal benefits of M. ptelefolia (MP). The present study reports antioxidant, anti-proliferative, and apoptosis induction activities of MP leaf extracts. METHOD: Young MP leaves were dried, powdered and extracted sequentially using hexane (HX), ethyl acetate (EA), methanol (MeOH) and water (W)...
May 5, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28467777/curcumin-induces-g2-m-cell-cycle-arrest-and-apoptosis-of-head-and-neck-squamous-cell-carcinoma-in-vitro-and-in-vivo-through-atm-chk2-p53-dependent-pathway
#14
An Hu, Jing-Juan Huang, Jing-Fei Zhang, Wei-Jun Dai, Rui-Lin Li, Zhao-Yang Lu, Jun-Li Duan, Ji-Ping Li, Xiao-Ping Chen, Jing-Ping Fan, Wei-Hua Xu, Hong-Liang Zheng
Studies have demonstrated that curcumin (CUR) exerts its tumor suppressor function in a variety of human cancers including head and neck squamous cell carcinoma (HNSCC). However, the exact underlying molecular mechanisms remain obscure. Here, we aim to test whether CUR affects ATM/Chk2/p53 signaling pathway, leading to the induction of cell cycle arrest, inhibition of angiogenesis of HNSCC in vitro and in vivo. To this end, we conducted multiple methods such as MTT assay, Invasion assay, Flow cytometry, Western blotting, RT-PCR, and transfection to explore the functions and molecular insights of CUR in HNSCC...
April 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28464995/in-vivo-reporter-gene-mutation-and-micronucleus-assays-in-gpt-delta-mice-treated-with-a-flame-retardant-decabromodiphenyl-ether
#15
Shinji Takasu, Yuji Ishii, Yuh Yokoo, Takuma Tsuchiya, Aki Kijima, Yukio Kodama, Kumiko Ogawa, Takashi Umemura
Polybrominated diphenyl ethers (PBDEs), a class of brominated flame retardants, have been widely used as additive flame retardants. Recently, the use of brominated flame retardants has been restricted or prohibited under various legislative acts because of the persistence, bioaccumulation potential, and toxicity of these compounds. However, there are also additional concerns regarding environmental contamination and human exposure to PBDEs resulting from informal recycling technology. Decabromodiphenyl ether (decaBDE), one type of PBDE, has carcinogenic potential in the livers of rodents...
April 2017: Mutation Research
https://www.readbyqxmd.com/read/28461573/genetic-and-small-molecule-disruption-of-the-aid-rad51-axis-similarly-protects-nonobese-diabetic-mice-from-type-1-diabetes-through-expansion-of-regulatory-b-lymphocytes
#16
Jeremy J Ratiu, Jeremy J Racine, Muneer G Hasham, Qiming Wang, Jane A Branca, Harold D Chapman, Jing Zhu, Nina Donghia, Vivek Philip, William H Schott, Clive Wasserfall, Mark A Atkinson, Kevin D Mills, Caroline M Leeth, David V Serreze
B lymphocytes play a key role in type 1 diabetes (T1D) development by serving as a subset of APCs preferentially supporting the expansion of autoreactive pathogenic T cells. As a result of their pathogenic importance, B lymphocyte-targeted therapies have received considerable interest as potential T1D interventions. Unfortunately, the B lymphocyte-directed T1D interventions tested to date failed to halt β cell demise. IgG autoantibodies marking humans at future risk for T1D indicate that B lymphocytes producing them have undergone the affinity-maturation processes of class switch recombination and, possibly, somatic hypermutation...
May 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28456641/low-density-lipoprotein-oxidation-biomarkers-in-human-health-and-disease-and-effects-of-bioactive-compounds
#17
REVIEW
Brigitte M Winklhofer-Roob, Gernot Faustmann, Johannes M Roob
Based on the significance of oxidized low-density lipoprotein (LDL) in health and disease, this review focuses on human studies addressing oxidation of LDL, including three lines of biomarkers, (i) ex vivo LDL resistance to oxidation, a "challenge test" model, (ii) circulating oxidized LDL, indicating the "current in vivo status", and (iii) autoantibodies against oxidized LDL as fingerprints of an immune response to oxidized LDL, along with circulating oxysterols and 4-hydroxynonenal as biomarkers of lipid peroxidation...
April 26, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28448631/biofortification-se-does-it-increase-the-content-of-phenolic-compounds-in-virgin-olive-oil-voo
#18
Roberto D'Amato, Primo Proietti, Andrea Onofri, Luca Regni, Sonia Esposto, Maurizio Servili, Daniela Businelli, Roberto Selvaggini
Extra-Virgin Olive Oil (EVOO) is a fundamental component of the Mediterranean diet and it may contain several anti-oxidant substances, such as phenols. Previous research has shown that this food may be enriched in phenols by spraying a sodium-selenate solution (100 mg L-1 Se) onto the crop canopy before flowering. The aim of this research was to evaluate the effect of this Se-fertilization before flowering (cv. Leccino) on the phenolic profile of EVOOs, and test to what extent such effects depend on the weather pattern, as observed in two contrasting experimental seasons (2013 and 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28447069/long-term-calorie-restriction-in-humans-is-not-associated-with-indices-of-delayed-immunologic-aging-a-descriptive-study
#19
A Janet Tomiyama, Jeffrey M Milush, Jue Lin, James M Flynn, Pankaj Kapahi, Eric Verdin, Elizabeth Sinclair, Simon Melov, Elissa S Epel
BACKGROUND: Delayed immunologic aging is purported to be a major mechanism through which calorie restriction (CR) exerts its anti-aging effects in non-human species. However, in non-obese humans, the effect of CR on the immune system has been understudied relative to its effects on the cardiometabolic system. OBJECTIVE: To examine whether CR is associated with delayed immunologic aging in non-obese humans. METHODS: We tested whether long-term CR practitioners (average 10.03 years of CR) evidenced decreased expression of T cell immunosenescence markers and longer immune cell telomeres compared to gender-, race/ethnicity-, age-, and education-matched "healthy" Body Mass Index (BMI) and "overweight"/"obese" BMI groups...
March 31, 2017: Nutrition and Healthy Aging
https://www.readbyqxmd.com/read/28446226/xenotransplantation-of-interferon-gamma-pretreated-clumps-of-a-human-mesenchymal-stem-cell-extracellular-matrix-complex-induces-mouse-calvarial-bone-regeneration
#20
Kei Takeshita, Souta Motoike, Mikihito Kajiya, Nao Komatsu, Manabu Takewaki, Kazuhisa Ouhara, Tomoyuki Iwata, Katsuhiro Takeda, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara
BACKGROUND: Three-dimensional cultured clumps of a mesenchymal stem cell (MSC)/extracellular matrix (ECM) complex (C-MSC) consists of cells and self-produced ECM. C-MSC can regulate the cellular function in vitro and induce successful bone regeneration using ECM as a cell scaffold. Potentiating the immunomodulatory capacity of C-MSCs, which can ameliorate the allo-specific immune response, may be helpful in developing beneficial "off-the-shelf" cell therapy for tissue regeneration. It is well reported that interferon (IFN)-γ stimulates the immunosuppressive properties of MSC via upregulation of the immunomodulatory enzyme IDO...
April 26, 2017: Stem Cell Research & Therapy
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