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Gene expression in carotid stenosis

Jian Dong, Chao Song, Lei Zhang, Xiang Feng, Rui Feng, Qingsheng Lu, Zhiqing Zhao, Junmin Bao, Jian Zhou, Zaiping Jing
As one of the leading reason in morbidity and death in the world, atherosclerosis is usually associated with vessel stenosis, ulceration, and inflammatory cell infiltration. However, the formation mechanism of atheroma plaque is unknown. In this research, we have used bioinformatics tools to identify 118 differential expression genes from a GEO dataset. Besides, we also revealed KYNU as a crucial gene in atheroma plaque development.
August 12, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Farima Zahedi, Maliheh Nazari-Jahantigh, Zhe Zhou, Pallavi Subramanian, Yuanyuan Wei, Jochen Grommes, Stefan Offermanns, Sabine Steffens, Christian Weber, Andreas Schober
MicroRNAs (miRNAs) coordinate vascular repair by regulating injury-induced gene expression in vascular smooth muscle cells (SMCs) and promote the transition of SMCs from a contractile to a proliferating phenotype. However, the effect of miRNA expression in SMCs on neointima formation is unclear. Therefore, we studied the role of miRNA biogenesis by Dicer in SMCs in vascular repair. Following wire-induced injury to carotid arteries of Apolipoprotein E knockout (Apoe (-/-)) mice, miRNA microarray analysis revealed that the most significantly regulated miRNAs, such as miR-222 and miR-21-3p, were upregulated...
September 12, 2016: Cellular and Molecular Life Sciences: CMLS
Zhen-Hua Zhou, Jing Peng, Zhao-You Meng, Lin Chen, Jia-Lu Huang, He-Qing Huang, Li Li, Wen Zeng, Yong Wei, Chu-Hong Zhu, Kang-Ning Chen
BACKGROUND: Carotid artery stenosis is a major risk factor for ischemic stroke. Although carotid angioplasty and stenting using an embolic protection device has been introduced as a less invasive carotid revascularization approach, in-stent restenosis limits its long-term efficacy and safety. The objective of this study was to test the anti-restenosis effects of local stent-mediated delivery of the A20 gene in a porcine carotid artery model. MATERIALS AND METHODS: The pCDNA3...
2016: Drug Design, Development and Therapy
S Kiando, N Tucker, L Castro-Vega, D Cusi, P Galan, Empana J-P, J Olin, H Gornik, P-F Plouin, I Kullo, D Milan, S Ganesh, P Boutouyrie, J Kovacic, X Jeunemaitre, N Bouatia-Naji
OBJECTIVE: Fibromuscular dysplasia (FMD) is a nonatherosclerotic vascular disease leading to stenosis, dissection and aneurysm affecting mainly the renal and cerebrovascular arteries. FMD has higher prevalence in females (80-90%) and predisposes to hypertension and stroke but its pathophysiology is unclear. DESIGN AND METHOD: We performed a multistage genetic association study (1,154 patients and 3,895 controls) from five case-control cohorts. We investigated the association between rs9349379 in the phosphatase and actin regulator 1 gene (PHACTR1) and carotid traits by echo-tracking in healthy volunteers, the expression of PHACTR1 by genotypes in human fibroblasts, protein staining pattern in human carotids, and vasculature development after Phactr1 knockdown in zebrafish...
September 2016: Journal of Hypertension
Jan-Marcus Daniel, Christoph A Reichel, Thomas Schmidt-Woell, Jochen Dutzmann, Gabriele Zuchtriegel, Fritz Krombach, Joerg Herold, Johann Bauersachs, Daniel G Sedding, Sandip M Kanse
BACKGROUND: The factor VII activating protease (FSAP) is a multifunctional circulating plasma serine protease involved in thrombosis and vascular remodelling processes. The Marburg I single nucleotide polymorphism (MI-SNP) in the FSAP-encoding gene is characterized by low proteolytic activity and is associated with increased stroke and carotid stenosis in humans. OBJECTIVES: We hypothesize that neointima formation after vascular injury is increased in FSAP(-/-) mice...
July 19, 2016: Journal of Thrombosis and Haemostasis: JTH
Sung Min Ahn, Yu Ri Kim, Ha Neui Kim, Yong-Il Shin, Hwa Kyoung Shin, Byung Tae Choi
We modeled prolonged cerebral hypoperfusion in mice using bilateral common carotid artery stenosis (BCAS) and electroacupuncture (EA) stimulation was applied at two acupoints, Baihui (GV20) and Dazhui (GV14). In behavioral tests of memory, BCAS produced impairments in spatial and short-term memory in mice that were attenuated by therapeutic EA stimulation. Therapeutic use of EA in BCAS also enhanced oligodendrocyte (OL) differentiation from oligodendrocyte precursor cells (OPCs), in association with white matter improvements in the corpus callosum (CC)...
2016: Scientific Reports
Ming Tan, Hong-Bing Yan, Jian-Nan Li, Wen-Ke Li, Yuan-Yuan Fu, Wen Chen, Zhou Zhou
BACKGROUND/AIMS: It is difficult to predict acute thrombotic cardiovascular events in the clinic. Few studies have reported the presence of plasma exosomes containing microRNAs (miRNAs) in cardiovascular events. Therefore, we aimed to investigate the levels of miR-223, miR-339 and miR-21 in plasma exosomes before thrombosis in mouse models of carotid tandem stenosis, as well as the mechanisms underlying the origin and function of these exosomal miRNAs. METHODS: Plasma samples were collected from the carotid tandem stenosis and sham control groups of our successfully developed atherothrombosis mouse models before thrombosis...
2016: Cellular Physiology and Biochemistry
Rajiv Janardhanan, Binxia Yang, Sreenivasulu Kilari, Edward B Leof, Debabrata Mukhopadhyay, Sanjay Misra
PURPOSE: To determine if a second dose of a lentivirus mediated small hairpin RNA that inhibits Vegf-A gene expression (LV-shRNA-Vegf-A) can improve lumen vessel area (LVA) of the outflow vein of an arteriovenous fistula (AVF) and decrease venous neointimal hyperplasia. MATERIALS AND METHODS: Chronic kidney disease was created in C57BL/6 mice; 28 days later, an AVF was created by connecting the right carotid artery to the ipsilateral jugular vein. Immediately after AVF creation, 5 × 10(6) plaque-forming units of LV-shRNA-Vegf-A or control shRNA was administered to the adventitia of the outflow vein, and a second dose of the same treatment was administered 14 days later...
April 2016: Journal of Vascular and Interventional Radiology: JVIR
S Kiando, N Tucker, A Katz, C Tréard, V Desca-Mard, L Castro-Vega, C Barlasina, D Cusi, P Galan, J Empana, J Olin, H Gornik, P Plouin, I Kullo, D Milan, S Ganesh, P Boutouyrie, J Kovacic, X Jeunemaitre, N Bouatia-Naji
BACKGROUND: Fibromuscular dysplasia (FMD) is a nonatherosclerotic vascular disease leading to arterial stenosis, aneurysm and dissection, mainly in renal and carotid artery. FMD has higher prevalence in females (80-90%) and is associated with hypertension and stroke. The pathophysiology of FMD is unclear and a genetic origin is suspected. METHODS: We performed a genetic association study in European ancestry individuals. The discovery included 249 cases and 689 controls, in which we analyzed 25,606 common variants (MAF>0...
December 2015: Annales de Cardiologie et D'angéiologie
Yu Zhang, Lin Cheng, Yajing Chen, Guo-Yuan Yang, Jianrong Liu, Lili Zeng
OBJECTIVE: We aim to explore the clinical factors and blood biomarker for predicting the early-onset post-stroke depression (PSD). METHODS: 251 acute ischemic stroke patients were divided into PSD group and non-PSD group by Hamilton depression scale in 2 weeks after stroke. The clinical data, the severity, etiology and location of stroke were recorded. The analysis of inflammatory mediator, glycose and lipid metabolism was performed on the day of admission. The association between clinical factors and early onset PSD was studied by logistic regression analysis...
March 15, 2016: Journal of Affective Disorders
L Perisic, S Aldi, Y Sun, L Folkersen, A Razuvaev, J Roy, M Lengquist, S Åkesson, C E Wheelock, L Maegdefessel, A Gabrielsen, J Odeberg, G K Hansson, G Paulsson-Berne, U Hedin
BACKGROUND: Embolism from unstable atheromas in the carotid bifurcation is a major cause of stroke. Here, we analysed gene expression in endarterectomies from patients with symptomatic (S) and asymptomatic (AS) carotid stenosis to identify pathways linked to plaque instability. METHODS: Microarrays were prepared from plaques (n = 127) and peripheral blood samples (n = 96) of S and AS patients. Gene set enrichment, pathway mapping and network analyses of differentially expressed genes were performed...
March 2016: Journal of Internal Medicine
Nicole J Ullrich, Leslie B Gordon
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare, uniformly fatal, segmental "premature aging" disease in which children exhibit phenotypes that may give us insights into the aging process at both the cellular and organismal levels. Initial presentation in early childhood is primarily based on growth and dermatologic findings. Primary morbidity and mortality for children with HGPS is from atherosclerotic cardiovascular disease and strokes with death occurring at an average age of 14.6 years...
2015: Handbook of Clinical Neurology
Mingyu Shi, Feng Yin, Hongyue Gu, Jing Zhu, Xinhua Yin
INTRODUCTION: Our aim was to study the efficacy and safety of tissue factor pathway inhibitor (TFPI)-coated stents in inhibiting restenosis in a rabbit carotid artery model. METHODS: Subculture was conducted in aorta smooth muscle cell, which was taken from male Wistar rat, and the 3-5-generation cells were taken for plasmid transfection and cytotoxicity experiment. TFPI microspheres were made of a TFPI plasmid which was enwrapped by poly-l-glutamic acid (PLGA)...
December 2015: Cardiovascular Therapeutics
Velidi H Rao, Vikrant Rai, Samantha Stoupa, Devendra K Agrawal
Although degradation of extracellular matrix by matrix metalloproteinases (MMPs) is thought to be involved in symptomatic (S) carotid plaques in atherosclerosis, the mechanisms of MMP expression are poorly understood. Here, we demonstrate that collagen loss in vascular smooth vessel cells (VSMCs) isolated from S plaques was induced by epidermal growth factor (EGF) through the activation of p38-MAPK and JNK-MAPK pathways. Inhibitors of p38-MAPK and JNK-MAPK signaling pathways downregulated the expression of MMP-1 and MMP-9...
September 15, 2015: American Journal of Physiology. Heart and Circulatory Physiology
Wenya Lan, Fang Yang, Zhuangli Li, Ling Liu, Hongfei Sang, Yongjun Jiang, Yunyun Xiong, Renliang Zhang
OBJECTIVE: Effective treatments against restenosis after percutaneous transluminal angioplasty and stenting are largely lacking. Human tissue kallikrein gene transfer has been shown to be able to attenuate neointima formation induced by balloon catheter. As a tissue kallikrein in vivo, human urinary kininogenase (HUK) is widely used to prevent ischemia-reperfusion injury. However, the effects of HUK on neointima formation have not been explored. We therefore investigated whether HUK could alleviate balloon catheter-induced intimal hyperplasia in rabbits fed with high-fat diets...
October 2016: Journal of Vascular Surgery
Hong Ran, Wei Duan, Zili Gong, Senlin Xu, He Zhu, Xianhua Hou, Li Jiang, Qifen He, Jian Zheng
Adenosine A2A receptors (A2ARs) in distinct cellular types may exert different and even opposite effects on many neurologic disorders; A2ARs in bone marrow-derived cells (BMDCs) have been shown to play important roles in various brain injuries. We previously showed that global A2AR inactivation aggravates chronic cerebral hypoperfusion-induced white matter lesions (WMLs); however, the specific cell populations responsible for A2AR-mediated signaling remain unknown. In the present study, we developed chimeric mice in which A2ARs were either selectively inactivated or reconstituted in BMDCs by transplanting bone marrow from global A2AR gene knockout or wild-type mice into wild-type or gene knockout mice, respectively...
April 2015: Journal of Neuropathology and Experimental Neurology
Azhar Abbas, Ida Gregersen, Sverre Holm, Isabelle Daissormont, Vigdis Bjerkeli, Kirsten Krohg-Sørensen, Karolina R Skagen, Tuva B Dahl, David Russell, Trine Almås, Dorte Bundgaard, Lars Holger Alteheld, Azita Rashidi, Christen P Dahl, Annika E Michelsen, Erik A Biessen, Pål Aukrust, Bente Halvorsen, Mona Skjelland
BACKGROUND AND PURPOSE: Interleukin (IL)-23 is a cytokine in the IL-12 family, mainly produced by antigen-presenting cells with a central role in inflammation. We hypothesize that IL-23 is also important in atherogenesis and investigate this in a population with carotid atherosclerosis. METHODS: Plasma levels of IL-23 were measured in patients with carotid artery stenosis and in healthy controls. The mRNA levels of IL-23 and its receptor, IL-23R, were measured in atherosclerotic plaques, nonatherosclerotic vessels, peripheral blood mononuclear cells, and plasmacytoid dendritic cells...
March 2015: Stroke; a Journal of Cerebral Circulation
Bhairavi Swaminathan, Haize Goikuria, Reyes Vega, Alfredo Rodríguez-Antigüedad, Antonio López Medina, María del Mar Freijo, Koen Vandenbroeck, Iraide Alloza
OBJECTIVES: The mechanism by which atheroma plaque becomes unstable is not completely understood to date but analysis of differentially expressed genes in stable versus unstable plaques may provide clues. This will be crucial toward disclosing the mechanistic basis of plaque instability, and may help to identify prognostic biomarkers for ischaemic events. The objective of our study was to identify differences in expression levels of 59 selected genes between symptomatic patients (unstable plaques) and asymptomatic patients (stable plaques)...
2014: PloS One
Vishal Singh, Minakshi Rana, Manish Jain, Niharika Singh, Arshi Naqvi, Richa Malasoni, Anil Kumar Dwivedi, Madhu Dikshit, Manoj Kumar Barthwal
In the present study, the anti-atherosclerotic effect and the underlying mechanism of curcuma oil (C. oil), a lipophilic fraction from turmeric (Curcuma longa L.), was evaluated in a hamster model of accelerated atherosclerosis and in THP-1 macrophages. Male golden Syrian hamsters were subjected to partial carotid ligation (PCL) or FeCl3-induced arterial oxidative injury (Ox-injury) after 1 week of treatment with a high-cholesterol (HC) diet or HC diet plus C. oil (100 and 300 mg/kg, orally). Hamsters fed with the HC diet were analysed at 1, 3 and 5 weeks following carotid injury...
January 14, 2015: British Journal of Nutrition
Young-Mi Go, Chan Woo Kim, Douglas I Walker, Dong Won Kang, Sandeep Kumar, Michael Orr, Karan Uppal, Arshed A Quyyumi, Hanjoong Jo, Dean P Jones
Disturbed blood flow (d-flow) occurring in branched and curved arteries promotes endothelial dysfunction and atherosclerosis, in part, by altering gene expression and epigenomic profiles in endothelial cells. While a systemic metabolic change is known to play a role in atherosclerosis, it is unclear whether it can be regulated by local d-flow. Here, we tested this hypothesis by carrying out a metabolomics study using blood plasma samples obtained from ApoE(-/-) mice that underwent a partial carotid ligation surgery to induce d-flow...
January 1, 2015: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
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