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Peripheral blood gene expression in ischemic stroke

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https://www.readbyqxmd.com/read/29739082/molecular-mechanism-exploration-of-ischemic-stroke-by-integrating-mrna-and-mirna-expression-profiles
#1
Jianhong Bao, Saijun Zhou, Sipei Pan, Yang Zhang
BACKGROUND: Stroke is one of the leading causes of death worldwide, and ischemic stroke accounts for about 80 percent of all cases. Ischemic stroke is usually caused by a blockage in an artery to the brain. However, its molecular mechanisms remain largely unclear. The integration of mRNA and miRNA expression patterns is an effective strategy to investigate the potential molecular mechanisms of ischemic stroke in posttranscriptional regulation level. METHODS: The mRNA and miRNA expression data were downloaded from the GEO database...
April 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29653195/genome-wide-integration-study-of-circulating-mirnas-and-peripheral-whole-blood-mrnas-of-male-acute-ischemic-stroke-patients
#2
Yang Xue, Pengqi Yin, Guozhong Li, Di Zhong
Several circulating microRNAs (miRNAs) have been proved to serve as stable biomarkers in blood for acute ischemic stroke (AIS). However, the functions of these biomarkers remain elusive. By conducting the integration analysis of circulating miRNAs and peripheral whole-blood mRNAs using bioinformatics methods, we explored the biological role of these circulating markers in peripheral whole blood at the genome-wide level. Stroke-related circulating miRNA profile data (GSE86291) and peripheral whole-blood mRNA expression data (GSE16561) were collected from the Gene Expression Omnibus (GEO) datasets...
June 1, 2018: Neuroscience
https://www.readbyqxmd.com/read/29622432/sirtuin-5-as-a-novel-target-to-blunt-blood-brain-barrier-damage-induced-by-cerebral-ischemia-reperfusion-injury
#3
Candela Diaz-Cañestro, Mario Merlini, Nicole R Bonetti, Luca Liberale, Patricia Wüst, Sylvie Briand-Schumacher, Jan Klohs, Sara Costantino, Melroy Miranda, Gabriele Schoedon-Geiser, Gerd A Kullak-Ublick, Alexander Akhmedov, Francesco Paneni, Jürg H Beer, Thomas F Lüscher, Giovanni G Camici
BACKGROUND: In acute ischemic stroke (AIS) patients, impaired blood-brain barrier (BBB) integrity is associated with hemorrhagic transformation and worsened outcome. Yet, the mechanisms underlying these relationships are poorly understood and consequently therapeutic strategies are lacking. This study sought to determine whether SIRT5 contributes to BBB damage following I/R brain injury. METHODS AND RESULTS: SIRT5 knockout (SIRT5-/- ) and wild type (WT) mice underwent transient middle cerebral artery (MCA) occlusion (tMCAO) followed by 48h of reperfusion...
June 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29550910/shifts-in-leukocyte-counts-drive-the-differential-expression-of-transcriptional-stroke-biomarkers-in-whole-blood
#4
Grant C O'Connell, Madison B Treadway, Connie S Tennant, Noelle Lucke-Wold, Paul D Chantler, Taura L Barr
Our group recently identified a panel of ten genes whose RNA expression levels in whole blood have utility for detection of stroke. The purpose of this study was to determine the mechanisms by which these genes become differentially expressed during stroke pathology. First, we assessed the transcriptional distribution of the ten genes across the peripheral immune system by measuring their expression levels on isolated neutrophils, monocytes, B-lymphocytes, CD-4+ T-lymphocytes, CD-8+ T-lymphocytes, and NK-cells generated from the blood of healthy donors (n = 3)...
March 17, 2018: Translational Stroke Research
https://www.readbyqxmd.com/read/29476556/n-myc-downstream-regulated-gene-2-protects-blood-brain-barrier-integrity-following-cerebral-ischemia
#5
Mika Takarada-Iemata, Akifumi Yoshikawa, Hieu Minh Ta, Nahoko Okitani, Takumi Nishiuchi, Yasuhiro Aida, Tomoya Kamide, Tsuyoshi Hattori, Hiroshi Ishii, Takashi Tamatani, Thuong Manh Le, Jureepon Roboon, Yasuko Kitao, Tomohiro Matsuyama, Mitsutoshi Nakada, Osamu Hori
Disruption of the blood-brain barrier (BBB) following cerebral ischemia is closely related to the infiltration of peripheral cells into the brain, progression of lesion formation, and clinical exacerbation. However, the mechanism that regulates BBB integrity, especially after permanent ischemia, remains unclear. Here, we present evidence that astrocytic N-myc downstream-regulated gene 2 (NDRG2), a differentiation- and stress-associated molecule, may function as a modulator of BBB permeability following ischemic stroke, using a mouse model of permanent cerebral ischemia...
February 24, 2018: Glia
https://www.readbyqxmd.com/read/29100048/novel-candidate-genes-of-the-park7-interactome-as-mediators-of-apoptosis-and-acetylation-in-multiple-sclerosis-an-in-silico-analysis
#6
George D Vavougios, Sotirios G Zarogiannis, Karen Angeliki Krogfelt, Konstantinos Gourgoulianis, Dimos Dimitrios Mitsikostas, Georgios Hadjigeorgiou
BACKGROUND: currently only 4 studies have explored the potential role of PARK7's dysregulation in MS pathophysiology Currently, no study has evaluated the potential role of the PARK7 interactome in MS. OBJECTIVE: The aim of our study was to assess the differential expression of PARK7 mRNA in peripheral blood mononuclears (PBMCs) donated from MS versus healthy patients using data mining techniques. METHODS: The PARK7 interactome data from the GDS3920 profile were scrutinized for differentially expressed genes (DEGs); Gene Enrichment Analysis (GEA) was used to detect significantly enriched biological functions...
January 2018: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/28932682/stroke-associated-pattern-of-gene-expression-previously-identified-by-machine-learning-is-diagnostically-robust-in-an-independent-patient-population
#7
Grant C O'Connell, Paul D Chantler, Taura L Barr
Our group recently employed genome-wide transcriptional profiling in tandem with machine-learning based analysis to identify a ten-gene pattern of differential expression in peripheral blood which may have utility for detection of stroke. The objective of this study was to assess the diagnostic capacity and temporal stability of this stroke-associated transcriptional signature in an independent patient population. Publicly available whole blood microarray data generated from 23 ischemic stroke patients at 3, 5, and 24 h post-symptom onset, as well from 23 cardiovascular disease controls, were obtained via the National Center for Biotechnology Information Gene Expression Omnibus...
December 2017: Genomics Data
https://www.readbyqxmd.com/read/28834871/bioinformatics-analysis-of-gene-expression-profiling-for-identification-of-potential-key-genes-among-ischemic-stroke
#8
Kaihua Zhai, Xiangli Kong, Boyu Liu, Jiyu Lou
This study aimed to identify the key differentially expressed genes (DEGs) following ischemic stroke (IS).The GSE22255 microarray dataset, which contains samples from peripheral blood mononuclear cells of 20 IS patients and 20 sex- and age-matched controls, was downloaded from the Gene Expression Omnibus. After data pre-processing, DEGs were identified using the Linear Models for Microarray Data package in R. The Search Tool for the Retrieval of Interacting Genes database was used to predict the interactions among the products of DEGs, and then Cytoscape software was used to visualize the protein-protein interaction (PPI) network...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28683362/promoter-hypomethylation-of-microrna223-gene-is-associated-with-atherosclerotic-cerebral-infarction
#9
Zhibin Li, Fang Yu, Xiaoqing Zhou, Sian Zeng, Qiong Zhan, Mei Yuan, Qidong Yang, Yunhai Liu, Jian Xia
BACKGROUND AND AIMS: microRNA223 (miR-223) plays an important role in the development of atherosclerosis and ischemic stroke. It is involved in regulation of multiple physiological and pathophysiological processes such as cholesterol metabolism, endothelial cell (EC) function, and thrombosis. Here we investigated the role of methylation regulation of MIR-223 promoter region in atherosclerotic cerebral infarction (ACI) patients. METHODS: A total of 23 patients with ACI and 32 healthy individuals were recruited...
June 28, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28469478/spatiotemporal-changes-in-p-glycoprotein-levels-in-brain-and-peripheral-tissues-following-ischemic-stroke-in-rats
#10
Kelly M DeMars, Changjun Yang, Kimberly E Hawkins, Austin O McCrea, David M Siwarski, Eduardo Candelario-Jalil
P-glycoprotein (P-gp) is known to transport a diverse array of xenobiotics, including therapeutic drugs. A member of the ATP-binding cassette (ABC) transporter family, P-gp is a protein encoded by the gene Mdr1 in humans and Abcb1 in rodents (represented by 2 isoforms Abcb1a and Abcb1b). Lining the luminal and abluminal membrane of brain capillary endothelial cells, P-gp is a promiscuous efflux pump extruding a variety of exogenous toxins and drugs. In this study, we measured dynamic changes in Abcb1a and Abcb1b transcripts and P-gp protein in the brain, liver, and kidney after experimental stroke...
2017: Journal of Experimental Neuroscience
https://www.readbyqxmd.com/read/28446746/peripheral-blood-akap7-expression-as-an-early-marker-for-lymphocyte-mediated-post-stroke-blood-brain-barrier-disruption
#11
Grant C O'Connell, Madison B Treadway, Ashley B Petrone, Connie S Tennant, Noelle Lucke-Wold, Paul D Chantler, Taura L Barr
Our group recently identified 16 genes whose peripheral blood expression levels are differentially regulated in acute ischemic stroke. The purpose of this study was to determine whether the early expression levels of any of these 16 genes are predictive for post-stroke blood brain barrier (BBB) disruption. Transcriptional expression levels of candidate genes were measured in peripheral blood sampled from ischemic stroke patients at emergency department admission, and BBB permeability was assessed at 24 hour follow up via perfusion-weighted imaging...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28222567/immune-biomarkers-for-the-diagnosis-of-mild-traumatic-brain-injury
#12
Ashley B Petrone, Valerie Gionis, Richard Giersch, Taura L Barr
BACKGROUND: In 2010, there were approximately 2.2 million emergency room visits associated with traumatic brain injury (TBI), with 80 percent diagnosed as mild TBI or concussion. In addition, there are a large number of TBIs, especially mild TBIs, which go either unreported by patients or initially undiagnosed by clinicians. Our team has previously identified a panel of immune-related genes that can diagnose ischemic stroke at triage, and due to shared pathophysiological mechanisms of TBI and stroke, we hypothesized that this panel of genes may also be utilized for the diagnosis of TBI...
2017: NeuroRehabilitation
https://www.readbyqxmd.com/read/27834745/altered-expression-of-long-noncoding-rnas-in-blood-after-ischemic-stroke-and-proximity-to-putative-stroke-risk-loci
#13
Cheryl Dykstra-Aiello, Glen C Jickling, Bradley P Ander, Natasha Shroff, Xinhua Zhan, DaZhi Liu, Heather Hull, Miles Orantia, Boryana S Stamova, Frank R Sharp
BACKGROUND AND PURPOSE: Although peripheral blood mRNA and micro-RNA change after ischemic stroke, any role for long noncoding RNA (lncRNA), which comprise most of the genome and have been implicated in various diseases, is unknown. Thus, we hypothesized that lncRNA expression also changes after stroke. METHODS: lncRNA expression was assessed in 266 whole-blood RNA samples drawn once per individual from patients with ischemic stroke and matched with vascular risk factor controls...
December 2016: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/27608956/interleukin-19-alleviates-brain-injury-by-anti-inflammatory-effects-in-a-mice-model-of-focal-cerebral-ischemia
#14
Weiying Xie, Lili Fang, Shuyuan Gan, Haojun Xuan
Stroke causes brain injury with neuroinflammation which exacerbates the neuronal damage. Recent studies show that anti-inflammatory cytokine interleukin-19 (IL-19) plays a critical part in the inflammatory and ischemic vascular diseases, yet its potential role in ischemic stroke is unknown. Here, we tested the hypothesis that IL-19 exerts protective effects against brain ischemia by modulating inflammation after stroke. Mice were injected intraperitoneally with 10ng/g per day recombinant mouse IL-19 starting pre-stroke, and were subjected to transient middle cerebral artery occlusion...
November 1, 2016: Brain Research
https://www.readbyqxmd.com/read/27412561/mir-145-mediated-the-role-of-aspirin-in-resisting-vsmcs-proliferation-and-anti-inflammation-through-cd40
#15
Xin Guo, Lijin Yu, Min Chen, Tian Wu, Xiangdong Peng, Ren Guo, Bikui Zhang
BACKGROUND: Aspirin (ASA) is the most widely used medicine to prevent cardiovascular diseases; however, the mechanisms by which ASA exerts its anti-proliferative effect remain not fully understood. This study was designed to investigate whether miR-145 is involved in the regulation of vascular smooth muscle cells' (VSMCs) proliferation and to determine the anti-inflammatory effects of ASA via its regulation of CD40 to provide a new theoretical basis for the pharmacological effect of aspirin...
July 13, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27407070/obtaining-human-ischemic-stroke-gene-expression-biomarkers-from-animal-models-a-cross-species-validation-study
#16
Yingying Wang, Yunpeng Cai
Recent studies have revealed the systematic altering of gene expression in human peripheral blood during the early stages of ischemic stroke, which suggests a new potential approach for the rapid diagnosis or prediction of stroke onset. Nevertheless, due to the difficulties of collecting human samples during proper disease stages, related studies are rather restricted. Many studies have instead been performed on manipulated animal models for investigating the regulation patterns of biomarkers during different stroke stages...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26900583/blood-transcriptome-changes-after-stroke-in-an-african-american-population
#17
Robert Meller, Andrea N Pearson, Jimmaline J Hardy, Casey L Hall, Dawn McGuire, Michael R Frankel, Roger P Simon
OBJECTIVE: Molecular diagnostic medicine holds much promise to change point of care treatment. An area where additional diagnostic tools are needed is in acute stroke care, to assist in diagnosis and prognosis. Previous studies using microarray-based gene expression analysis of peripheral blood following stroke suggests this approach may be effective. Next-generation sequencing (NGS) approaches have expanded genomic analysis and are not limited to previously identified genes on a microarray chip...
February 2016: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/26856463/analysis-of-association-between-map2k4-gene-polymorphism-rs3826392-and-il-1b-serum-level-in-southern-chinese-han-ischemic-stroke-patients
#18
Lian Gu, Yanli Wu, Shuyan Hu, Qing Chen, Jinjing Tan, Yan Yan, Baoyun Liang, Nong Tang
BACKGROUND: Mitogen-activated protein kinase kinase 4 (MAP2K4) gene acts as the direct upstream activator of c-Jun NH2-terminal kinase pathway, which plays an important role in regulating neuron survival and apoptosis in response to cerebral ischemia. However, the association between MAP2K4 gene polymorphisms and ischemic stroke (IS) has not yet been published. Therefore, this study investigates the association between MAP2K4 gene polymorphism rs3826392 and IS susceptibility, as well as its quantitative traits in Southern Chinese Han population...
May 2016: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/26846866/peripheral-blood-mcemp1-gene-expression-as-a-biomarker-for-stroke-prognosis
#19
MULTICENTER STUDY
Kripa Raman, Martin J O'Donnell, Anna Czlonkowska, Yan Carlos Duarte, Patricio Lopez-Jaramillo, Ernesto Peñaherrera, Mike Sharma, Ashkan Shoamanesh, Marta Skowronska, Salim Yusuf, Guillaume Paré
BACKGROUND AND PURPOSE: A limitation when making early decisions on stroke management is the lack of rapid diagnostic and prognostic testing. Our study sought to identify peripheral blood RNA biomarkers associated with stroke. The secondary aims were to assess the discriminative capacity of RNA biomarkers for primary stroke type and stroke prognosis at 1-month. METHODS: Whole-blood gene expression profiling was conducted on the discovery cohort: 129 first-time stroke cases that had blood sampling within 5 days of symptom onset and 170 control participants with no history of stroke...
March 2016: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/25124890/a-genomic-profile-of-the-immune-response-to-stroke-with-implications-for-stroke-recovery
#20
Taura L Barr, Reyna VanGilder, Stephanie Rellick, Steven D Brooks, Danielle N Doll, Ann Noelle Lucke-Wold, Dongquan Chen, James Denvir, Steven Warach, Andrew Singleton, Mar Matarin
OBJECTIVES: The objectives of this study were to determine the change in gene expression between two time points following stroke and to identify biomarkers of stroke recovery through gene expression profiling and pathway analysis. METHODS: Peripheral blood was collected from 34 ischemic stroke patients (confirmed by magnetic resonance imaging) ≥18 years of age, within 24 hr of symptom onset and 24-48 hr later, and from healthy controls. The Modified Rankin Scale (MRS) was used to determine 30-day recovery...
May 2015: Biological Research for Nursing
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