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Single-cell RNA FISH tuberculosis

Pooja Vir, Riccardo Arrigucci, Karim Lakehal, Amy L Davidow, Richard Pine, Sanjay Tyagi, Yuri Bushkin, Alfred Lardizabal, Maria Laura Gennaro
RNA flow cytometry (FISH-Flow) achieves high-throughput measurement of single-cell gene expression by combining in-situ nucleic acid hybridization with flow cytometry. We tested whether antigen-specific T-cell responses detected by FISH-Flow correlated with latent tuberculosis infection (LTBI), a condition affecting one-third of the world population. Peripheral-blood mononuclear cells from donors, identified as positive or negative for LTBI by current medical practice, were stimulated ex vivo with mycobacterial antigen...
2015: PloS One
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