keyword
https://read.qxmd.com/read/38507562/adverse-environmental-perturbations-may-threaten-kelp-farming-sustainability-by-exacerbating-enterobacterales-diseases
#21
JOURNAL ARTICLE
Yongyu Zhang, Shailesh Nair, Zenghu Zhang, Jiulong Zhao, Hanshuang Zhao, Longfei Lu, Lirong Chang, Nianzhi Jiao
Globally kelp farming is gaining attention to mitigate land-use pressures and achieve carbon neutrality. However, the influence of environmental perturbations on kelp farming remains largely unknown. Recently, a severe disease outbreak caused extensive kelp mortality in Sanggou Bay, China, one of the world's largest high-density kelp farming areas. Here, through in situ investigations and simulation experiments, we find indications that an anomalously dramatic increase in elevated coastal seawater light penetration may have contributed to dysbiosis in the kelp Saccharina japonica 's microbiome...
March 20, 2024: Environmental Science & Technology
https://read.qxmd.com/read/38506518/bacterial-interactions-on-nutrient-rich-surfaces-in-the-gut-lumen
#22
REVIEW
Bo Huey Chiang, Giovanni Vega, Sarah C Dunwoody, Michael L Patnode
The intestinal lumen is a turbulent, semi-fluid landscape where microbial cells and nutrient-rich particles are distributed with high heterogeneity. Major questions regarding the basic physical structure of this dynamic microbial ecosystem remain unanswered. Most gut microbes are non-motile, and it is unclear how they achieve optimum localization relative to concentrated aggregations of dietary glycans that serve as their primary source of energy. In addition, a random spatial arrangement of cells in this environment is predicted to limit sustained interactions that drive co-evolution of microbial genomes...
March 20, 2024: Infection and Immunity
https://read.qxmd.com/read/38505530/staphylococcus-aureus-panton-valentine-leukocidin-worsens-acute-implant-associated-osteomyelitis-in-humanized-brgsf-mice
#23
JOURNAL ARTICLE
Marloes I Hofstee, Claudia Siverino, Motoo Saito, Himanshu Meghwani, James Tapia-Dean, Samson Arveladze, Maria Hildebrand, Javier Rangel-Moreno, Martijn Riool, Stephan Zeiter, Sebastian A J Zaat, T Fintan Moriarty, Gowrishankar Muthukrishnan
Staphylococcus aureus is the most common pathogen that causes implant-associated osteomyelitis, a clinically incurable disease. Immune evasion of S. aureus relies on various mechanisms to survive within the bone niche, including the secretion of leukotoxins such as Panton-Valentine leukocidin (PVL). PVL is a pore-forming toxin exhibiting selective human tropism for C5a receptors (C5aR1 and C5aR2) and CD45 on neutrophils, monocytes, and macrophages. PVL is an important virulence determinant in lung, skin and soft tissue infections...
February 2024: JBMR Plus
https://read.qxmd.com/read/38501873/alternate-typhoid-toxin-assembly-evolved-independently-in-the-two-salmonella-species
#24
JOURNAL ARTICLE
Antonio J Chemello, Casey C Fowler
UNLABELLED: AB5 -type toxins are a diverse family of protein toxins composed of an enzymatic active (A) subunit and a pentameric delivery (B) subunit. Salmonella enterica serovar Typhi's typhoid toxin features two A subunits, CdtB and PltA, in complex with the B subunit PltB. Recently, it was shown that S . Typhi encodes a horizontally acquired B subunit, PltC, that also assembles with PltA/CdtB to produce a second form of typhoid toxin. S . Typhi therefore produces two AB5 toxins with the same A subunits but distinct B subunits, an evolutionary twist that is unique to typhoid toxin...
March 19, 2024: MBio
https://read.qxmd.com/read/38501820/cultivating-efficiency-high-throughput-growth-analysis-of-anaerobic-bacteria-in-compact-microplate-readers
#25
JOURNAL ARTICLE
Oona L O Snoeyenbos-West, Christina R Guerrero, Makaela Valencia, Paul Carini
UNLABELLED: Anaerobic microbes play crucial roles in environmental processes, industry, and human health. Traditional methods for monitoring the growth of anaerobes, including plate counts or subsampling broth cultures for optical density measurements, are time and resource-intensive. The advent of microplate readers revolutionized bacterial growth studies by enabling high-throughput and real-time monitoring of microbial growth kinetics. Yet, their use in anaerobic microbiology has remained limited...
March 19, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38497255/the-roles-of-abcb1-p-glycoprotein-drug-transporters-in-regulating-gut-microbes-and-inflammation-insights-from-animal-models-old-and-new
#26
REVIEW
Lauren Stoeltje, Jenna K Luc, Timothaus Haddad, Catherine S Schrankel
Commensal enteric bacteria have evolved systems that enable growth in the ecologic niche of the host gastrointestinal tract. Animals evolved parallel mechanisms to survive the constant exposure to bacteria and their metabolic by-products. We propose that drug transporters encompass a crucial system to managing the gut microbiome. Drug transporters are present in the apical surface of gut epithelia. They detoxify cells from small molecules and toxins (xenobiotics) in the lumen. Here, we review what is known about commensal structure in the absence of the transporter ABCB1/P-glycoprotein in mammalian models...
May 6, 2024: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://read.qxmd.com/read/38495512/modulation-of-growth-microcystin-production-and-algal-bacterial-interactions-of-the-bloom-forming-algae-microcystis-aeruginosa-by-a-novel-bacterium-recovered-from-its-phycosphere
#27
JOURNAL ARTICLE
Yao Xiao, Mijia Du, Yang Deng, Qinglin Deng, Xin Wang, Yiwen Yang, Binghuo Zhang, Yu-Qin Zhang
Harmful algal blooms (HABs) in natural waters are of escalating global concern due to their detrimental impact on environmental health. Emerging evidence indicates that algae-bacteria symbionts can affect HAB features, though much about this interplay remains largely unexplored. The current study isolated a new species of Mucilaginibacter (type strain JXJ CY 39T ) from culture biomass of the bloom-causing Microcystis aeruginosa FACHB-905 (Maf) from Lake Dianchi, China. Strain JXJ CY 39T was an aerobic, Gram-stain-negative rod bacterium that grew at 5-38°C, pH 4...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38493438/development-of-a-sensor-for-disulfide-bond-formation-in-diverse-bacteria
#28
JOURNAL ARTICLE
Dyotima, Sally Abulaila, Jocelyne Mendoza, Cristina Landeta
UNLABELLED: In bacteria, disulfide bonds contribute to the folding and stability of proteins important for processes in the cellular envelope. In Escherichia coli , disulfide bond formation is catalyzed by DsbA and DsbB enzymes. DsbA is a periplasmic protein that catalyzes disulfide bond formation in substrate proteins, while DsbB is an inner membrane protein that transfers electrons from DsbA to quinones, thereby regenerating the DsbA active state. Actinobacteria including mycobacteria use an alternative enzyme named VKOR, which performs the same function as DsbB...
March 13, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38493146/structural-basis-for-autoinhibition-by-the-dephosphorylated-regulatory-domain-of-ycf1
#29
JOURNAL ARTICLE
Nitesh Kumar Khandelwal, Thomas M Tomasiak
Yeast Cadmium Factor 1 (Ycf1) sequesters glutathione and glutathione-heavy metal conjugates into yeast vacuoles as a cellular detoxification mechanism. Ycf1 belongs to the C subfamily of ATP Binding Cassette (ABC) transporters characterized by long flexible linkers, notably the regulatory domain (R-domain). R-domain phosphorylation is necessary for activity, whereas dephosphorylation induces autoinhibition through an undefined mechanism. Because of its transient and dynamic nature, no structure of the dephosphorylated Ycf1 exists, limiting understanding of this R-domain regulation...
March 16, 2024: Nature Communications
https://read.qxmd.com/read/38487883/antimicrobial-resistance-and-virulence-profiling-of-staphylococcus-pseudintermedius-isolated-from-cats-bangladesh
#30
JOURNAL ARTICLE
Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman
Staphylococcus pseudintermedius is a significant bacterial pathogen that frequently colonizes different body sites and mucous membranes of pets. The objectives of the cross-sectional study were to estimate the prevalence, antimicrobial resistance pattern, and detection of diverse resistance as well as virulence genes of S. pseudintermedius in cats. A standard bacteriological method, species-specific gene and different antimicrobial resistance as well as virulence genes were confirmed by PCR assay. A total of 233 swab samples were collected from different body sites of 102 cats, among them 146 swabs from 73 healthy cats, and 87 from 29 diseased cats...
December 2024: Veterinary Quarterly
https://read.qxmd.com/read/38486702/detecting-and-characterizing-new-endofungal-bacteria-in-new-hosts-pandoraea-sputorum-and-mycetohabitans-endofungorum-in-rhizopus-arrhizus
#31
JOURNAL ARTICLE
Xiao-Ling Liu, Heng Zhao, Yi-Xin Wang, Xin-Ye Liu, Yang Jiang, Meng-Fei Tao, Xiao-Yong Liu
The fungus Rhizopus arrhizus (= R. oryzae ) is commonly saprotrophic, exhibiting a nature of decomposing organic matter. Additionally, it serves as a crucial starter in food fermentation and can act as a pathogen causing mucormycosis in humans and animals. In this study, two distinct endofungal bacteria (EFBs), associated with individual strains of R. arrhizus , were identified using live/dead staining, fluorescence in situ hybridization, transmission electron microscopy, and 16S rDNA sequencing. The roles of these bacteria were elucidated through antibiotic treatment, pure cultivation, and comparative genomics...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38486387/identification-of-genes-encoded-toxin-antitoxin-system-in-mycobacterium-tuberculosis-strains-from-clinical-sample
#32
JOURNAL ARTICLE
Karthikeyan Sundaram, Leela Kagithakara Vajravelu, Ravichandiran Velayutham, Utpal Mohan
BACKGROUND: The toxin-antitoxin system is a genetic element that is highly present in Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis. The toxin-antitoxin sys-tem comprises toxin protein and antitoxin protein or non-encoded RNA interacting with each other and inhibiting toxin activity. M. Tuberculosis has more classes of TA loci than non-tubercle bacilli and other microbes, including VapBC, HigBA, MazEF, ParDE, RelBE, MbcTA, PemIK, DarTG, MenTA, one tripartite type II TAC chaperone system, and hypothetical proteins...
March 14, 2024: Infectious Disorders Drug Targets
https://read.qxmd.com/read/38485702/unveiling-the-hidden-players-exploring-the-role-of-gut-mycobiome-in-cancer-development-and-treatment-dynamics
#33
REVIEW
Lingxi Li, Xiaowen Huang, Haoyan Chen
The role of gut fungal species in tumor-related processes remains largely unexplored, with most studies still focusing on fungal infections. This review examines the accumulating evidence suggesting the involvement of commensal and pathogenic fungi in cancer biological process, including oncogenesis, progression, and treatment response. Mechanisms explored include fungal influence on host immunity, secretion of bioactive toxins/metabolites, interaction with bacterial commensals, and migration to other tissues in certain types of cancers...
2024: Gut Microbes
https://read.qxmd.com/read/38485446/response-of-the-toxic-dinoflagellate-alexandrium-minutum-to-exudates-of-the-eelgrass-zostera-marina
#34
JOURNAL ARTICLE
Alexandra Díaz-Alonso, Francisco Rodríguez, Pilar Riobó, Xose Álvarez-Salgado, Eva Teira, Emilio Fernández
Biotic interactions are a key factor in the development of harmful algal blooms. Recently, a lower abundance of planktonic dinoflagellates has been reported in areas dominated by seagrass beds, suggesting a negative interaction between both groups of organisms. The interaction between planktonic dinoflagellates and marine phanerogams, as well as the way in which bacteria can affect this interaction, was studied in two experiments using a non-axenic culture of the toxic dinoflagellate Alexandrium minutum exposed to increasing additions of eelgrass (Zostera marina) exudates from old and young leaves and to the presence or absence of antibiotics...
March 2024: Harmful Algae
https://read.qxmd.com/read/38484487/effect-of-high-sodium-intake-on-gut-tight-junctions-structure-and-permeability-to-bacterial-toxins-in-a-rat-model-of-chronic-kidney-disease
#35
JOURNAL ARTICLE
María de la Luz Villela-Torres, María-Del-Carmen Prado-Uribe, Marcela Ávila Díaz, Héctor Quezada Pablo, Elizabeth Soria-Castro, Nuria Esturau Escofet, Catalina Elizabeth Flores Maldonado, Ramón Paniagua
INTRODUCTION: Uremic toxicity changes the gut structure and permeability, allowing bacterial toxins to translocate from the lumen to the blood during chronic kidney failure (CKD). Clinical fluid overload and tissue edema without uremia have similar effects but have not been adequately demonstrated and analyzed in CKD. AIMS: To investigate the effect of sodium intake on the plasma concentration of gut-derived uremic toxins, indoxyl sulfate (IS), and p-cresyl sulfate (pCS) and the expression of genes and proteins of epithelial gut tight junctions in a rat model of CKD...
March 13, 2024: Archives of Medical Research
https://read.qxmd.com/read/38483683/characterization-and-genome-informatic-analysis-of-a-novel-lytic-mendocina-phage-vb_pmes_stp12-suitable-for-phage-therapy-pseudomonas-or-biocontrol
#36
JOURNAL ARTICLE
Sani Sharif Usman, Evangeline Christina
BACKGROUND: A novel lytic bacteriophage (phage) was isolated with Pseudomonas mendocina strain STP12 (P. mendocina) from the untreated site of Sewage Treatment Plant of Lovely Professional University, India. P. mendocina is a Gram-negative, rod-shaped, aerobic bacterium belonging to the family Pseudomonadaceae and has been reported in fifteen (15) cases of economically important diseases worldwide. METHODS AND RESULTS: Here, a novel phage specifically infecting and killing P...
March 14, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38482615/intestinal-epithelial-cell-specific-knockout-of-mettl3-aggravates-intestinal-inflammation-in-clp-mice-by-weakening-the-intestinal-barrier
#37
JOURNAL ARTICLE
Hongzhou Shi, Jiahui Sun, Yaya Sun, Junjie Wu, Guangqing Jiang, Zhaiyue Xu, Xin Shi, Miao Fang
BACKGROUND: Many studies have demonstrated that the expression of methyltransferase- like 3 (METTL3) is altered in various inflammatory diseases. Its specific mechanistic role in the intestinal inflammatory response during sepsis remains limited and requires further investigation. OBJECTIVES: Explore the potential mechanism of METTL3 in the intestinal inflammatory response during sepsis. MATERIALS AND METHODS: Immunohistochemical analysis was utilized to detect the expression of METTL3 in the necrotic intestine of patients with intestinal necrosis and the small intestine of cecal ligation and puncture (CLP) mice...
March 13, 2024: Current Pharmaceutical Biotechnology
https://read.qxmd.com/read/38482361/clinical-impact-and-public-health-challenges-of-a-pvl-mrsa-bacteraemia-outbreak-amongst-people-who-inject-drugs-in-south-yorkshire-uk
#38
JOURNAL ARTICLE
Matthew Beaumont, Bala Subramanian, Ken Agwuh, Yan Ryan, Bruno Pichon
BACKGROUND: Panton-Valentine leukocidin (PVL) Staphylococcus aureus (SA) is an emergent public health concern. PVL toxin has been mostly associated with methicillin-sensitive S. aureus (MSSA)-related skin and soft tissue infections occurring in high-risk groups such as people who inject drugs (PWID). The emergence of PVL methicillin-resistant S. aureus (MRSA) infection is causing severe and life-threatening disease in PWID. CLINICAL CASES: We present an outbreak of eight PVL-MRSA bacteraemia cases at a UK teaching hospital between 2018 and 2022...
2024: Access microbiology
https://read.qxmd.com/read/38481790/prevalent-role-of-homologous-recombination-in-the-repair-of-specific-double-strand-breaks-in-rhizobium-etli
#39
JOURNAL ARTICLE
Fares Osam Yáñez-Cuna, Diana Aguilar-Gómez, Araceli Dávalos, David Romero
Double-strand breaks (DSBs) are the most dangerous injuries for a genome. When unrepaired, death quickly ensues. In most bacterial systems, DSBs are repaired through homologous recombination. Nearly one-quarter of bacterial species harbor a second system, allowing direct ligation of broken ends, known as Non-Homologous End Joining (NHEJ). The relative role of both systems in DSBs repair in bacteria has been explored only in a few cases. To evaluate this in the bacterium Rhizobium etli , we used a modified version of the symbiotic plasmid (264 kb), containing a single copy of the nifH gene...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38479597/kcsa-kv1-x-chimeras-with-complete-ligand-binding-sites-provide-improved-predictivity-for-screening-selective-kv1-x-blockers
#40
JOURNAL ARTICLE
Patrik Szekér, Tamás Bodó, Katalin Klima, Ágota Csóti, Nikoletta Ngo Hanh, József Murányi, Anna Hajdara, Tibor Gábor Szántó, György Panyi, Márton Megyeri, Zalán Péterfi, Sándor Farkas, Norbert Gyöngyösi, Péter Hornyák
Despite significant advances in the development of therapeutic interventions targeting autoimmune diseases and chronic inflammatory conditions, lack of effective treatment still poses a high unmet need. Modulating chronically activated T cells through the blockade of the Kv1.3 potassium channel is a promising therapeutic approach, however, developing selective Kv1.3 inhibitors is still an arduous task. Phage display-based high throughput peptide library screening is a rapid and robust approach to develop promising drug candidates; however, it requires solid-phase immobilization of target proteins with their binding site preserved...
March 11, 2024: Journal of Biological Chemistry
keyword
keyword
64309
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.