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https://www.readbyqxmd.com/read/29748128/the-effects-of-mitochondrial-inhibitors-on-ca-2-signalling-and-electrical-conductances-required-for-pacemaking-in-interstitial-cells-of-cajal-in-the-mouse-small-intestine
#1
Bernard T Drumm, Tae S Sung, Haifeng Zheng, Salah A Baker, Sang D Koh, Kenton M Sanders
Interstitial cells of Cajal (ICC-MY) are pacemakers that generate and propagate electrical slow waves in gastrointestinal (GI) muscles. Slow waves appear to be generated by the release of Ca2+ from intracellular stores and activation of Ca2+ -activated Cl- channels (Ano1). Conduction of slow waves to smooth muscle cells coordinates rhythmic contractions. Mitochondrial Ca2+ handling is currently thought to be critical for ICC pacemaking. Protonophores, inhibitors of the electron transport chain (FCCP, CCCP or antimycin) or mitochondrial Na+ /Ca2+ exchange blockers inhibited slow waves in several GI muscles...
February 1, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29747396/beta-estradiol-regulates-voltage-gated-calcium-channels-and-estrogen-receptors-in-telocytes-from-human-myometrium
#2
Adela Banciu, Daniel Dumitru Banciu, Cosmin Catalin Mustaciosu, Mihai Radu, Dragos Cretoiu, Junjie Xiao, Sanda Maria Cretoiu, Nicolae Suciu, Beatrice Mihaela Radu
Voltage-gated calcium channels and estrogen receptors are essential players in uterine physiology, and their association with different calcium signaling pathways contributes to healthy and pathological conditions of the uterine myometrium. Among the properties of the various cell subtypes present in human uterine myometrium, there is increasing evidence that calcium oscillations in telocytes (TCs) contribute to contractile activity and pregnancy. Our study aimed to evaluate the effects of beta-estradiol on voltage-gated calcium channels and estrogen receptors in TCs from human uterine myometrium and to understand their role in pregnancy...
May 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29739832/expression-of-calcium-channel-transcripts-in-the-zebrafish-heart-dominance-of-t-type-channels
#3
Jaakko Haverinen, Minna Hassinen, Surjya Narayan Dash, Matti Vornanen
Calcium (Ca) channels are necessary for cardiac excitation-contraction (e-c) coupling, but Ca channel composition of fish hearts is still largely unknown. To this end, we determined transcript expression of Ca channels in the heart of zebrafish ( Danio rerio ), a popular model species. Altogether 18 Ca channel α-subunit genes were expressed in both atrium and ventricle. Transcripts for 7 L-type (Cav1.1a, Cav1.1b, Cav1.2, Cav1.3a, Cav1.3b, Cav1.4a, Cav1.4b), 5 T-type (Cav3.1, Cav3.2a, Cav3.2b, Cav3.3a, Cav3...
May 8, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29720258/t-type-calcium-channels-functionally-interact-with-spectrin-%C3%AE-%C3%AE-and-ankyrin-b
#4
Agustin Garcia-Caballero, Fang-Xiong Zhang, Victoria Hodgkinson, Junting Huang, Lina Chen, Ivana A Souza, Stuart Cain, Jennifer Kass, Sascha Alles, Terrance P Snutch, Gerald W Zamponi
This study describes the functional interaction between the Cav3.1 and Cav3.2 T-type calcium channels and cytoskeletal spectrin (α/β) and ankyrin B proteins. The interactions were identified utilizing a proteomic approach to identify proteins that interact with a conserved negatively charged cytosolic region present in the carboxy-terminus of T-type calcium channels. Deletion of this stretch of amino acids decreased binding of Cav3.1 and Cav3.2 calcium channels to spectrin (α/β) and ankyrin B and notably also reduced T-type whole cell current densities in expression systems...
May 2, 2018: Molecular Brain
https://www.readbyqxmd.com/read/29557990/vascular-restenosis-in-coronary-artery-bypass-grafting-might-be-associated-with-vegf-c-vegfr-3-signaling-pathway
#5
Zuzanna Podemska-Jedrzejczak, Agnieszka Malinska, Patrycja Sujka-Kordowska, Michal Nowicki, Mateusz Puslecki, Marek Jemielity, Bartlomiej Perek
The vascular endothelial growth factor (VEGF) family of peptides and caveolins (CAVs) are reported to contribute, in early graft failure in patients, a coronary artery bypass grafting (CABG). To investigate the possible association of ultimate luminal occlusion to VEGFs and CAVs expression, a functional analysis (based on the molecular biology, bioinformatics, histology, and clinical studies) was performed. Twenty-four hundred and sixty-eight CABG patients diagnosed with multivessel stable coronary artery disease (CAD) were enrolled into prospective study and assigned to two subgroups: double- and triple-vessel CAD subjects...
March 20, 2018: Heart and Vessels
https://www.readbyqxmd.com/read/29526554/differential-control-of-axonal-and-somatic-resting-potential-by-voltage-dependent-conductances-in-cortical-layer-5-pyramidal-neurons
#6
Wenqin Hu, Bruce P Bean
Voltage-dependent conductances not only drive action potentials but also help regulate neuronal resting potential. We found differential regulation of resting potential in the proximal axon of layer 5 pyramidal neurons compared to the soma. Axonal resting potential was more negative than the soma, reflecting differential control by multiple voltage-dependent channels, including sodium channels, Cav3 channels, Kv7 channels, and HCN channels. Kv7 current is highly localized to the axon and HCN current to the soma and dendrite...
March 21, 2018: Neuron
https://www.readbyqxmd.com/read/29492218/cav3-1-overexpression-is-associated-with-negative-characteristics-and-prognosis-in-non-small-cell-lung-cancer
#7
Aleksi Suo, Allison Childers, Adrijana D'Silva, Lars F Petersen, Shannon Otsuka, Michelle Dean, Haocheng Li, Emeka K Enwere, Brant Pohorelic, Alexander Klimowicz, Ivana A Souza, Jawed Hamid, Gerald W Zamponi, DGwyn Bebb
Introduction: Voltage-gated calcium channels (VGCC) have been found to be differentially expressed in several different tumor types, but their role in tumor growth, malignant invasion, metastases and impact on clinical outcomes has not been clarified. Materials and Methods: From a cohort database of 193 patients with early-stage NSCLC, 163 formalin-fixed paraffin-embedded specimens were available for analysis to construct tissue microarrays. Cav3.1 protein expression was detected using fluorescence immunohistochemistry, and quantified using automated image acquisition and analysis...
February 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29486470/abnormal-downregulation-of-caveolin-3-mediates-the-pro-fibrotic-action-of-microrna-22-in-a-model-of-myocardial-infarction
#8
Lu Zhang, Hongli Yin, Lei Jiao, Tianyi Liu, Yuqiu Gao, Yingchun Shao, Yuanyuan Zhang, Hongli Shan, Ying Zhang, Baofeng Yang
BACKGROUND/AIMS: Cardiac fibrosis is an important cardiac remodeling event that can ultimately lead to the development of severe arrhythmia and heart failure. MicroRNAs (miRNAs) are involved in the pathogenesis of many cardiovascular diseases. Here, we aimed to investigate the effects of caveolin-3 (Cav3) on the pathogenesis of cardiac fibrosis and the underlying molecular mechanisms. METHODS: Cav3 expression was decreased in cardiac fibrosis in vivo and in vitro model...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29474447/contribution-of-s4-segments-and-s4-s5-linkers-to-the-low-voltage-activation-properties-of-t-type-cav3-3-channels
#9
Ana Laura Sanchez-Sandoval, Zazil Herrera Carrillo, Clara Estela Díaz Velásquez, Dulce María Delgadillo, Heriberto Manuel Rivera, Juan Carlos Gomora
Voltage-gated calcium channels contain four highly conserved transmembrane helices known as S4 segments that exhibit a positively charged residue every third position, and play the role of voltage sensing. Nonetheless, the activation range between high-voltage (HVA) and low-voltage (LVA) activated calcium channels is around 30-40 mV apart, despite the high level of amino acid similarity within their S4 segments. To investigate the contribution of S4 voltage sensors for the low-voltage activation characteristics of CaV3...
2018: PloS One
https://www.readbyqxmd.com/read/29463073/t-type-calcium-channels-are-required-to-maintain-viability-of-neural-progenitor-cells
#10
Ji-Woon Kim, Hyun Ah Oh, Sung Hoon Lee, Ki Chan Kim, Pyung Hwa Eun, Mee Jung Ko, Edson Luck T Gonzales, Hana Seung, Seonmin Kim, Geon Ho Bahn, Chan Young Shin
T-type calcium channels are low voltage-activated calcium channels that evoke small and transient calcium currents. Recently, T-type calcium channels have been implicated in neurodevelopmental disorders such as autism spectrum disorder and neural tube defects. However, their function during embryonic development is largely unknown. Here, we investigated the function and expression of T-type calcium channels in embryonic neural progenitor cells (NPCs). First, we compared the expression of T-type calcium channel subtypes (CaV3...
February 21, 2018: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/29407215/involvement-of-the-cystathionine-%C3%AE-lyase-ca-v-3-2-pathway-in-substance-p-induced-bladder-pain-in-the-mouse-a-model-for-nonulcerative-bladder-pain-syndrome
#11
Maho Tsubota, Yasumasa Okawa, Yuhei Irie, Mariko Maeda, Tomoka Ozaki, Fumiko Sekiguchi, Hiroyasu Ishikura, Atsufumi Kawabata
Hydrogen sulfide (H2 S) formed by cystathionine-γ-lyase (CSE) enhances the activity of Cav 3.2 T-type Ca2+ channels, contributing to the bladder pain accompanying hemorrhagic cystitis caused by systemic administration of cyclophosphamide (CPA) in mice. Given clinical and fundamental evidence for the involvement of the substance P/NK1 receptor systems in bladder pain syndrome (BPS)/interstitial cystitis (IC), we created an intravesical substance P-induced bladder pain model in mice and analyzed the possible involvement of the CSE/Cav 3...
May 1, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29402774/trpc3-overexpression-promotes-the-progression-of-inflammation-induced-preterm-labor-and-inhibits-t-cell-activation
#12
Chen Jing, Zheng Dongming, Cui Hong, Na Quan, Liu Sishi, Liu Caixia
BACKGROUND/AIMS: To detect the expression of the TRPC3 channel protein in the tissues of women experiencing preterm labor and investigate its interaction with T lymphocytes, providing a theoretical basis for the clinical prevention of threatened preterm labor and the development of drug-targeted therapy. METHODS: Forty-seven women experiencing preterm labor and 47 women experiencing normal full-term labor were included in this study. All included women underwent delivery via cesarean section; uterine samples were obtained at delivery...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29401592/kir2-inward-rectification-controlled-precise-and-dynamic-balances-between-kir2-and-hcn-currents-initiate-pacemaking-activity
#13
Kuihao Chen, Dongchuan Zuo, Sho-Ya Wang, Haijun Chen
Spontaneous rhythmic action potential or pacemaking activity of pacemaker cells controls rhythmic signaling such as heartbeat. The mechanism underlying the origin of pacemaking activity is not well understood. In this study, we created human embryonic kidney (HEK) 293 cells that show pacemaking activity through heterologous expression of strong inward rectifier K+ channels (Kir2.1), hyperpolarization-activated cyclic nucleotide-gated nonselective cation channels (HCN2), and voltage-gated Na+ or Ca2+ channels (Nav 1...
January 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29385656/t-type-calcium-channels-drive-migration-invasion-in-brafv600e-melanoma-cells-through-snail1
#14
Oscar Maiques, Carla Barceló, Anaïs Panosa, Jordi Pijuan, Jose L Orgaz, Irene Rodriguez-Hernandez, Clara Matas-Nadal, Gemma Tell, Ramón Vilella, Angels Fabra, Susana Puig, Victoria Sanz-Moreno, Xavier Matias-Guiu, Carles Canti, Judit Herreros, Rosa M Marti, Anna Macià
Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3...
January 31, 2018: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/29327607/up-regulation-of-cav3-1-expression-in-sh-sy5y-cells-induced-by-lidocaine-hydrochloride
#15
Qin Gong, Xianjie Wen, Heng Li, Jian He, Yunhua Wang, Huiping Wu, Hanbing Wang, Xiaoping Wang
BACKGROUND: Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride. METHODS: The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively...
January 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29326130/inward-rectifier-potassium-channels-kir2-x-and-caveolin-3-domain-specific-interaction-implications-for-purkinje-cell-dependent-ventricular-arrhythmias
#16
Ravi Vaidyanathan, Hanora Van Ert, Kazi T Haq, Stefano Morotti, Samuel Esch, Elise C McCune, Eleonora Grandi, Lee L Eckhardt
BACKGROUND: In human cardiac ventricle, I K1 is mainly comprised Kir2.1, but Kir2.2 and Kir2.3 heterotetramers occur and modulate I K1 . Long-QT syndrome-9-associated CAV3 mutations cause decreased Kir2.1 current density, but Kir2.x heterotetramers have not been studied. Here, we determine the effect of long-QT syndrome-9- CAV3 mutation F97C on Kir2.x homo- and heterotetramers and model-associated arrhythmia mechanisms. METHODS AND RESULTS: Super-resolution microscopy, co-immunoprecipitation, cellular electrophysiology, on-cell Western blotting, and simulation of Purkinje and ventricular myocyte mathematical models were used...
January 2018: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/29311306/mechanism-specific-assay-design-facilitates-the-discovery-of-nav1-7-selective-inhibitors
#17
Tania Chernov-Rogan, Tianbo Li, Gang Lu, Henry Verschoof, Kuldip Khakh, Steven W Jones, Maureen H Beresini, Chang Liu, Daniel F Ortwine, Steven J McKerrall, David H Hackos, Daniel Sutherlin, Charles J Cohen, Jun Chen
Many ion channels, including Nav1.7, Cav1.3, and Kv1.3, are linked to human pathologies and are important therapeutic targets. To develop efficacious and safe drugs, subtype-selective modulation is essential, but has been extremely difficult to achieve. We postulate that this challenge is caused by the poor assay design, and investigate the Nav1.7 membrane potential assay, one of the most extensively employed screening assays in modern drug discovery. The assay uses veratridine to activate channels, and compounds are identified based on the inhibition of veratridine-evoked activities...
January 23, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29304398/generation-of-human-induced-pluripotent-stem-cells-euraci001-a-euraci002-a-euraci003-a-from-peripheral-blood-mononuclear-cells-of-three-patients-carrying-mutations-in-the-cav3-gene
#18
Viviana Meraviglia, Patrizia Benzoni, Sara Landi, Carmen Murano, Marianna Langione, Benedetta M Motta, Serena Baratto, Rosamaria Silipigni, Marina Di Segni, Peter P Pramstaller, Dario DiFrancesco, Elisabetta Gazzerro, Andrea Barbuti, Alessandra Rossini
Caveolinopathies are a heterogeneous family of genetic pathologies arising from alterations of the caveolin-3 gene (CAV3), encoding for the isoform specifically constituting muscle caveolae. Here, by reprogramming peripheral blood mononuclear cells, we report the generation of induced pluripotent stem cells (iPSCs) from three patients carrying the ΔYTT deletion, T78K and W101C missense mutations in caveolin-3. iPSCs displayed normal karyotypes and all the features of pluripotent stem cells in terms of morphology, specific marker expression and ability to differentiate in vitro into the three germ layers...
March 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29274077/increasing-t-type-calcium-channel-activity-by-%C3%AE-adrenergic-stimulation-contributes-to-%C3%AE-adrenergic-regulation-of-heart-rates
#19
Yingxin Li, Xiaoxiao Zhang, Chen Zhang, Xiaoying Zhang, Ying Li, Zhao Qi, Christopher Szeto, Mingxin Tang, Yizhi Peng, Jeffery D Molkentin, Steven R Houser, Mingxing Xie, Xiongwen Chen
Cav3.1 T-type Ca2+ channel current (ICa-T ) contributes to heart rate genesis but is not known to contribute to heart rate regulation by the sympathetic/β-adrenergic system (SAS). We show that the loss of Cav3.1 makes the beating rates of the heart in vivo and perfused hearts ex vivo, as well as sinoatrial node cells, less sensitive to β-adrenergic stimulation; it also renders less conduction acceleration through the atrioventricular node by β-adrenergic stimulation. Increasing Cav3.1 in cardiomyocytes has the opposite effects...
April 1, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29262379/dexamethasone-induced-upregulation-of-ca-v-3-2-t-type-ca-2-channels-in-rat-cardiac-myocytes
#20
D Falcón, R González-Montelongo, E Sánchez de Rojas-de Pedro, A Ordóñez, J Ureña, A Castellano
Glucocorticoids are widely used to treat acute and chronic diseases. Unfortunately, their therapeutic use is associated with severe side effects. Glucocorticoids are known to regulate several ion channels in cardiac myocytes, including voltage-dependent Ca2+ channels. Low-voltage-activated T-type Ca2+ channels are expressed in ventricular myocytes during the fetal and perinatal period, but are practically absent in the adult. However, these channels can be re-expressed in adult cardiomyocytes under some pathological conditions...
April 2018: Journal of Steroid Biochemistry and Molecular Biology
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