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phenylalanine metabolism

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https://www.readbyqxmd.com/read/28220590/biosynthesis-and-mass-spectral-fragmentation-pathways-of-13-c-and-15-n-labeled-cytochalasin-d-produced-by-xylaria-arbuscula
#1
Luciana da Silva Amaral, Taicia Pacheco Fill, Luiz Fernando Arruda Santos, Edson Rodrigues-Filho
The fungus Xylaria arbuscula was isolated as an endophyte from Cupressus lusitanica and has shown to be a prominent producer of cytochalasans, mainly cytochalasin C, D and Q. Cytochalasans comprise an important class of fungal secondary metabolites that have aroused attention due to their uncommon molecular structures and pronounced biological activities. Due to the few published studies on the ESI-MS/MS fragmentation of this important class of secondary metabolites, in the first part of our work we studied the cytochalasin D fragmentation pathways using an ESI-Q-ToF mass spectrometer coupled with liquid chromatography...
February 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28203327/a-rapid-screening-test-on-dried-blood-for-the-neonatal-diagnosis-of-tyrosinemia-type-i
#2
Farahnaz Bodaghkhan, Bita Geramizadeh, Abbas Abdollah Rajeh, Mahmoud Haghighat, Mohsen Dehghani, Naser Honar, Mojgan Zahmatkeshan, Mohammad-Hadi Imanieh
BACKGROUND: Tyrosinemia is an inherited metabolic disorder characterized by elevated levels of tyrosine and its metabolites in plasma. Without treatment, the disease will progress to hepatic and renal failure, so that without liver transplantation will cause death in less than 10 years of age. So, early diagnosis and treatment can be life saving and crucial. It means that with early treatment starting in the neonatal period, the patient can have normal life with very few restrictions in diets containing tyrosine and phenylalanine...
October 2016: Iranian Journal of Pediatrics
https://www.readbyqxmd.com/read/28201992/high-flavonoid-accompanied-with-high-starch-accumulation-triggered-by-nutrient-starvation-in-bioenergy-crop-duckweed-landoltia-punctata
#3
Xiang Tao, Yang Fang, Meng-Jun Huang, Yao Xiao, Yang Liu, Xin-Rong Ma, Hai Zhao
BACKGROUND: As the fastest growing plant, duckweed can thrive on anthropogenic wastewater. The purple-backed duckweed, Landoltia punctata, is rich in starch and flavonoids. However, the molecular biological basis of high flavonoid and low lignin content remains largely unknown, as does the best method to combine nutrients removed from sewage and the utilization value improvement of duckweed biomass. RESULTS: A combined omics study was performed to investigate the biosynthesis of flavonoid and the metabolic flux changes in L...
February 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28199114/curcumin-loaded-blood-stable-polymeric-micelles-for-enhancing-therapeutic-effect-on-erythroleukemia
#4
Feirong Gong, Dan Chen, Xin Teng, Junhua Ge, Xianfeng Ning, Ya-Ling Shen, Jian Li, Shanfeng Wang
Curcumin has high potential in suppressing many types of cancer and overcoming multi-drug resistance in a multi-faceted manner by targeting diverse molecular targets. However, the rather low systemic bioavailability resulted from its extremely low aqueous solubility and rapid metabolism and excretion in vivo has hampered its application in cancer therapy. With the primary aim to increase the aqueous solubility of curcumin while retaining its stability in the blood circulation before it accesses the tumor site, here we report preparation of curcumin-loaded N-(tert-butoxycarbonyl)-L-phenylalanine end-capped methoxy-poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL-Phe(Boc)) micelles with high stability both in vitro and in vivo and anti-tumor efficacy...
February 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28197155/dissecting-the-biochemical-and-transcriptomic-effects-of-a-locally-applied-heat-treatment-on-developing-cabernet-sauvignon-grape-berries
#5
Fatma Lecourieux, Christian Kappel, Philippe Pieri, Justine Charon, Jérémy Pillet, Ghislaine Hilbert, Christel Renaud, Eric Gomès, Serge Delrot, David Lecourieux
Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196863/conformational-changes-in-the-activation-loop-of-mitochondrial-glutaminase-c-a-direct-fluorescence-read-out-that-distinguishes-the-binding-of-allosteric-inhibitors-from-activators
#6
Clint A Stalnecker, Jon W Erickson, Richard A Cerione
The first step in glutamine catabolism is catalyzed by the mitochondrial enzyme glutaminase, with a specific isoform, glutaminase C (GAC), being highly expressed in cancer cells. GAC activation requires the formation of homo-tetramers, promoted by anionic allosteric activators such as inorganic phosphate. This leads to the proper orientation of a flexible loop proximal to the dimer-dimer interface that is essential for catalysis (i.e. the activation loop). A major class of allosteric inhibitors of GAC, with the prototype being BPTES (bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide), and the related molecule CB-839, binds to the activation loop and induces the formation of an inactive tetramer (2 inhibitors bound per active tetramer)...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28187322/chronic-aspartame-intake-causes-changes-in-the-trans-sulphuration-pathway-glutathione-depletion-and-liver-damage-in-mice
#7
Isabela Finamor, Salvador Pérez, Caroline A Bressan, Carlos E Brenner, Sergio Rius-Pérez, Patricia C Brittes, Gabriele Cheiran, Maria I Rocha, Marcelo da Veiga, Juan Sastre, Maria A Pavanato
No-caloric sweeteners, such as aspartame, are widely used in various food and beverages to prevent the increasing rates of obesity and diabetes mellitus, acting as tools in helping control caloric intake. Aspartame is metabolized to phenylalanine, aspartic acid, and methanol. Our aim was to study the effect of chronic administration of aspartame on glutathione redox status and on the trans-sulphuration pathway in mouse liver. Mice were divided into three groups: control; treated daily with aspartame for 90 days; and treated with aspartame plus N-acetylcysteine (NAC)...
February 1, 2017: Redox Biology
https://www.readbyqxmd.com/read/28166636/cartilaginous-metabolomic-study-reveals-potential-mechanisms-of-osteophyte-formation-in-osteoarthritis
#8
Zhongwei Xu, Tingmei Chen, Jiao Luo, Shijia Ding, Sichuan Gao, Jian Zhang
Osteophyte is one of the inevitable consequences of progressive osteoarthritis with the main characteristics of cartilage degeneration and endochondral ossification. The pathogenesis of osteophyte formation is not fully understood to date. In this work, metabolomic approaches were employed to explore potential mechanisms of osteophyte formation by detecting metabolic variations between extracts of osteophyte cartilage tissues (n = 32) and uninvolved control cartilage tissues (n = 34), based on the platform of ultraperformance liquid chromatography tandem quadrupole time-of-flight mass spectrometry, as well as the use of multivariate statistic analysis and univariate statistic analysis...
February 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28165881/subtle-visuomotor-deficits-and-reduced-benefit-from-practice-in-early-treated-phenylketonuria
#9
Claudia Caprile, Jaume Campistol, Laura Puigcerver, Alfonso-Pablo Gutiérrez-Mata, Itziar Alonso-Colmenero, Roser Colomé, Jordi Navarra
INTRODUCTION: Phenylketonuria (PKU) is a rare metabolic disease that causes slight-to-severe neurological symptoms. Slow performance has been observed in PKU but the influence of high-order (i.e., not purely motor) deficits and of temporary variations of the phenylalanine (Phe) level on this slowness has not been fully corroborated as yet. Response speed and the effect of motor practice during the performance of a visuomotor coordination task were measured, in a group of patients with early-treated phenylketonuria (ET PKU)...
February 6, 2017: Journal of Clinical and Experimental Neuropsychology
https://www.readbyqxmd.com/read/28165429/the-dynamics-of-the-defense-strategy-of-pea-induced-by-exogenous-nitric-oxide-in-response-to-aphid-infestation
#10
Agnieszka Woźniak, Magda Formela, Piotr Bilman, Katarzyna Grześkiewicz, Waldemar Bednarski, Łukasz Marczak, Dorota Narożna, Katarzyna Dancewicz, Van Chung Mai, Beata Borowiak-Sobkowiak, Jolanta Floryszak-Wieczorek, Beata Gabryś, Iwona Morkunas
The aim of this study was to investigate the effect of exogenous nitric oxide (NO), i.e., S-nitrosoglutathione (GSNO) and sodium nitroprusside (SNP), on the metabolic status of Pisum sativum L. cv. Cysterski leaves infested by Acyrthosiphon pisum Harris, population demographic parameters and A. pisum feeding activity. A reduction in the level of semiquinone radicals in pea seedling leaves pretreated with exogenous NO occurred 24 h after A. pisum infestation, which was earlier than in non-pretreated leaves. A decrease in the level of O₂(•-) was observed in leaves pretreated with GSNO and infested by aphids at 48 and 72 h post-infestation (hpi)...
February 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28152597/gc-ms-metabolomics-identifies-metabolite-alterations-that-precede-subclinical-mastitis-in-the-blood-of-transition-dairy-cows
#11
Elda Dervishi, Guanshi Zhang, Suzanna M Dunn, Rupasri Mandal, David S Wishart, Burim N Ametaj
The objectives of this study were to determine alterations in the serum metabolites related to amino acid (AA), carbohydrate, and lipid metabolism in transition dairy cows before diagnosis of subclinical mastitis (SCM), during, and after diagnosis of disease. A subclinical mastitis case was determined as a cow having somatic cell count (SCC) > 200 000/mL of milk for two or more consecutive reports. Blood samples were collected from 100 Holstein dairy cows at five time points at -8 and -4 weeks before parturition, at the week of SCM diagnosis, and +4 and +8 weeks after parturition...
February 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28148297/transcriptome-analysis-of-cucumis-sativus-infected-by-cucurbit-chlorotic-yellows-virus
#12
Xinyan Sun, Zhenyue Wang, Qinsheng Gu, Honglian Li, Weili Han, Yan Shi
BACKGROUND: Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus-host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis. METHODS: We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection. RESULTS: CCYV infection resulted in 865 DEGs...
February 2, 2017: Virology Journal
https://www.readbyqxmd.com/read/28146081/p-cresyl-sulfate
#13
REVIEW
Tessa Gryp, Raymond Vanholder, Mario Vaneechoutte, Griet Glorieux
If chronic kidney disease (CKD) is associated with an impairment of kidney function, several uremic solutes are retained. Some of these exert toxic effects, which are called uremic toxins. p-Cresyl sulfate (pCS) is a prototype protein-bound uremic toxin to which many biological and biochemical (toxic) effects have been attributed. In addition, increased levels of pCS have been associated with worsening outcomes in CKD patients. pCS finds its origin in the intestine where gut bacteria metabolize aromatic amino acids, such as tyrosine and phenylalanine, leading to phenolic end products, of which pCS is one of the components...
January 29, 2017: Toxins
https://www.readbyqxmd.com/read/28139496/therapeutic-efficacy-of-an-elemental-diet-for-patients-with-crohn-s-disease-and-its-association-with-amino-acid-metabolism
#14
Masakazu Nakano, Keiichi Tominaga, Atsushi Hoshino, Takeshi Sugaya, Kazunari Kanke, Hideyuki Hiraishi
BACKGROUND/AIM: We investigated the association between blood amino acid concentration changes caused by elemental diet (ED) and their relationship to its therapeutic effect. PATIENTS AND METHODS: Patients with active Crohn's disease (CD) followed ED for 12 weeks. Patients not previously treated with ED were defined as new ED, and those with previous ED therapy (≥900 kcal/day) were defined as previous ED. Disease activity markers [Crohn's disease activity index (CDAI) and C-reactive protein (CRP) level], blood biochemistry test results, and plasma amino acid concentrations were measured before and after the treatment...
January 2017: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/28132861/global-and-untargeted-metabolomics-evidence-of-the-protective-effect-of-different-extracts-of-dipsacus-asper-wall-ex-c-b-clarke-on-estrogen-deficiency-after-ovariectomia-in-rats
#15
Yi Tao, Xi Chen, Weidong Li, Baochang Cai, Liuqing Di, Liyun Shi, Lihong Hu
ETHNOPHARMACOLOGICAL RELEVANCE: Dipsacus asper Wall. ex C.B. Clarke (DA) is an effectively traditional Chinese medicine for treating osteoporosis and bone fracture. Until now, studies on pharmacological mechanism of DA mostly centered on cell and gene level, little is known about its metabolic signatures. The aim of this study is to investigate the anti-osteoporosis effects of crude and wine-processed DA by global and untargeted metabolic profiling of rats serum, liver and kidney derived from sham, model and treated groups...
January 26, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28132689/biallelic-mutations-in-dnajc12-cause-hyperphenylalaninemia-dystonia-and-intellectual-disability
#16
Yair Anikster, Tobias B Haack, Thierry Vilboux, Ben Pode-Shakked, Beat Thöny, Nan Shen, Virginia Guarani, Thomas Meissner, Ertan Mayatepek, Friedrich K Trefz, Dina Marek-Yagel, Aurora Martinez, Edward L Huttlin, Joao A Paulo, Riccardo Berutti, Jean-François Benoist, Apolline Imbard, Imen Dorboz, Gali Heimer, Yuval Landau, Limor Ziv-Strasser, May Christine V Malicdan, Corinne Gemperle-Britschgi, Kirsten Cremer, Hartmut Engels, David Meili, Irene Keller, Rémy Bruggmann, Tim M Strom, Thomas Meitinger, James C Mullikin, Gerard Schwartz, Bruria Ben-Zeev, William A Gahl, J Wade Harper, Nenad Blau, Georg F Hoffmann, Holger Prokisch, Thomas Opladen, Manuel Schiff
Phenylketonuria (PKU, phenylalanine hydroxylase deficiency), an inborn error of metabolism, can be detected through newborn screening for hyperphenylalaninemia (HPA). Most individuals with HPA harbor mutations in the gene encoding phenylalanine hydroxylase (PAH), and a small proportion (2%) exhibit tetrahydrobiopterin (BH4) deficiency with additional neurotransmitter (dopamine and serotonin) deficiency. Here we report six individuals from four unrelated families with HPA who exhibited progressive neurodevelopmental delay, dystonia, and a unique profile of neurotransmitter deficiencies without mutations in PAH or BH4 metabolism disorder-related genes...
February 2, 2017: American Journal of Human Genetics
https://www.readbyqxmd.com/read/28130717/impact-of-dietary-intake-on-bone-turnover-in-patients-with-phenylalanine-hydroxylase-deficiency
#17
Kathryn E Coakley, Eric I Felner, Vin Tangpricha, Peter W F Wilson, Rani H Singh
: Phenylalanine hydroxylase (PAH) deficiency is a genetic disorder characterized by deficiency of the PAH enzyme. Patients follow a phenylalanine-restricted diet low in intact protein, and must consume synthetic medical food (MF) to supply phenylalanine-free protein. We assessed relationships between dietary intake and nutrient source (food or MF) on bone mineral density (BMD) and bone turnover markers (BTM) in PAH deficiency. Blood from 44 fasted females 11-52 years of age was analyzed for plasma phenylalanine, serum BTM [CTx (resorption), P1NP (formation)], vitamin D, and parathyroid hormone (PTH)...
January 28, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28129369/serum-metabolomics-of-burkitt-lymphoma-mouse-models
#18
Fengmin Yang, Jie Du, Hong Zhang, Guorui Ruan, Junfeng Xiang, Lixia Wang, Hongxia Sun, Aijiao Guan, Gang Shen, Yan Liu, Xiaomeng Guo, Qian Li, Yalin Tang
Burkitt lymphoma (BL) is a rare and highly aggressive type of non-Hodgkin lymphoma. The mortality rate of BL patients is very high due to the rapid growth rate and frequent systemic spread of the disease. A better understanding of the pathogenesis, more sensitive diagnostic tools and effective treatment methods for BL are essential. Metabolomics, an important aspect of systems biology, allows the comprehensive analysis of global, dynamic and endogenous biological metabolites based on their nuclear magnetic resonance (NMR) and mass spectrometry (MS)...
2017: PloS One
https://www.readbyqxmd.com/read/28129265/parenteral-and-enteral-nutrition-in-surgical-critical-care-plasma-metabolomics-demonstrates-divergent-effects-on-nitrogen-fatty-acid-ribonucleotide-and-oxidative-metabolism
#19
Brodie A Parent, Max Seaton, Danijel Djukovic, Haiwei Gu, Brittany Wheelock, Daniel Raftery, Grant E O'Keefe
BACKGROUND: Artificial nutrition support is central to the care of critically ill patients and is primarily provided enterally (EN). There are circumstances when parenteral nutrition (PN) is considered necessary. We are uncertain how each of these approaches confer clinical benefits beyond simply providing calories. We sought to better understand how each of these techniques influence metabolism in critically-ill patients using a broad-based metabolomics approach. Metabolic responses to EN and PN may differ in ways that could help us understand how to optimize use of these therapies...
January 26, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28123992/sodium-dithionite-enhanced-quality-of-radix-scutellariae-through-modification-of-secondary-metabolism
#20
Guo Huimin, Fu Xiaoying, Du Hongwei, Cong Wei, Meng Xiangcai
INTRODUCTION: The quality of radix scutellariae is particularly associated with environmental stresses, but detailed mechanisms remained unclear. Plant under unfavorable situation generates redundant reactive oxygen species (ROS), and ROS can modify the secondary metabolism. The varied quality of radix scutellariae could be explained by ROS. MATERIALS AND METHODS: .004, 0.4, and 40 μmol/L of sodium dithionite (Na2S2O4), a material producing ROS, were applied to Scutellaria baicalensis to mimic unfavorable situation...
October 2016: International Journal of Pharmaceutical Investigation
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