Xue Liu, Xiao-Bo Li, Jianlan Jiang, Zhen-Ning Liu, Bin Qiao, Fei-Fei Li, Jing-Sheng Cheng, Xiaocui Sun, Ying-Jin Yuan, Jianjun Qiao, Guang-Rong Zhao
Synthetic microbial coculture to express heterologous biosynthetic pathway for de novo production of medicinal ingredients is an emerging strategy for metabolic engineering and synthetic biology. Here, taking efficient production of salidroside as an example of glycosides, we design and construct a syntrophic Escherichia coli-E. coli coculture composed of the aglycone (AG) strain and the glycoside (GD) strain, which convergently accommodate biosynthetic pathways of tyrosol and salidroside, respectively. To accomplish this the phenylalanine-deficient AG strain was engineered to utilize xylose preferentially and to overproduce precursor tyrosol, while the tyrosine-deficient GD strain was constructed to consume glucose exclusively and to enhance another precursor UDP-glucose availability for synthesis of salidroside...
March 26, 2018: Metabolic Engineering