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phenylalanine metabolism

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https://www.readbyqxmd.com/read/28089618/24-hour-protein-arginine-and-citrulline-metabolism-in-fed-critically-ill-children-a-stable-isotope-tracer-study
#1
Carlijn T I de Betue, Xiomara C Garcia Casal, Dick A van Waardenburg, Stephen M Schexnayder, Koen F M Joosten, Nicolaas E P Deutz, Marielle P K J Engelen
BACKGROUND & AIMS: The reference method to study protein and arginine metabolism in critically ill children is measuring plasma amino acid appearances with stable isotopes during a short (4-8 h) time period and extrapolate results to 24-h. However, 24-h measurements may be variable due to critical illness related factors and a circadian rhythm could be present. Since only short duration stable isotope studies in critically ill children have been conducted before, the aim of this study was to investigate 24-h appearance of specific amino acids representing protein and arginine metabolism, with stable isotope techniques in continuously fed critically ill children...
January 4, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28080075/cognitive-outcomes-in-early-treated-adults-with-phenylketonuria-pku-a-comprehensive-picture-across-domains
#2
Liana Palermo, Tarekegn Geberhiwot, Anita MacDonald, Ellie Limback, S Kate Hall, Cristina Romani
OBJECTIVE: Phenylketonuria (PKU) is an inherited metabolic disease which affects cognitive functions due to an inability to metabolize phenylalanine which leads to the accumulation of toxic by-products (Phe) in the brain. PKU can be effectively treated with a low phenylalanine diet, but some cognitive deficits remain. Studies have reported impairments, especially for processing speed and executive functions, but there is a lack of comprehensive assessment across cognitive domains. Moreover, it is important to establish outcomes in early treated adults with PKU (AwPKU) who have better metabolic control than groups previously reported in the literature...
January 12, 2017: Neuropsychology
https://www.readbyqxmd.com/read/28063302/metabonomics-study-of-the-therapeutic-mechanism-of-fenugreek-galactomannan-on-diabetic-hyperglycemia-in-rats-by-ultra-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#3
Wenyue Jiang, Lu Gao, Pengdong Li, Hong Kan, Jiale Qu, Lihui Men, Zhiqiang Liu, Zhongying Liu
Fenugreek is a traditional plant for the treatment of diabetes. Galactomannan, an active major component in fenugreek seeds, has shown hypoglycemic activity. The present study was performed to investigate the therapeutic mechanism underlying fenugreek galactomannan (F-GAL) in treating diabetes, using a metabonomics approach based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The F-GAL used for study was highly purified, and its yield, purity, and galactose/mannose ratio were characterized by capillary zone electrophoresis (CZE) and a modified phenol-sulfuric acid method...
December 29, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28058725/urinary-metabolomics-of-complete-freund-s-adjuvant-induced-hyperalgesia-in-rats
#4
Yang Liu, Hui Chen, Jun Lu, Youshui Jiang, Rui Yang, Songyan Gao, Xin Dong, Wei Chen
The aim of this study was to demonstrate the differences of metabolomics changes in a hyperalgesia model and find potent biomarkers of hyperalgesia. Seven rats were placed in metabolic cages. An emulsion containing 500 μg of Complete Freund's adjuvant (CFA) was used to induce hyperalgesia. Urine samples were collected prior to the injection of CFA and on post-injection days 1, 3 and 7. Ultraperformance liquid chromatography, coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS), was used for a quantitative analysis of urinary metabolic changes in the CFA-induced hyperalgesia model...
November 5, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28056396/transcriptome-profiling-of-sweetpotato-tuberous-roots-during-low-temperature-storage
#5
Chang Yoon Ji, Won-Hyong Chung, Ho Soo Kim, Won Yong Jung, Le Kang, Jae Cheol Jeong, Sang-Soo Kwak
Sweetpotato [Ipomoea batatas (L.) Lam] is a globally important root crop with high industrial value. However, because sweetpotato tuberous roots undergo chilling injuries that negatively affect their quality at temperatures below 10 °C, postharvest damage during the winter season is a major constraint for industrialization. To understand chilling injury response during postharvest low temperature storage, we used next-generation sequencing technology to comprehensive analyze the transcriptome of tuberous roots stored at optimal (13 °C) or low temperature (4 °C) for 6 weeks...
December 24, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28053823/global-metabolic-profiling-of-plasma-shows-that-three-year-mild-caloric-restriction-lessens-an-age-related-increase-in-sphingomyelin-and-reduces-l-leucine-and-l-phenylalanine-in-overweight-and-obese-subjects
#6
Minjoo Kim, Sang-Hyun Lee, Jong Ho Lee
The effect of weight loss from long-term, mild-calorie diets (MCD) on plasma metabolites is unknown. This study was to examine whether MCD-induced weight reduction caused changes in the extended plasma metabolites. Overweight and obese subjects aged 40-59 years consumed a MCD (approximately 100 kcal/day deficit, n=47) or a weight-maintenance diet (control, n=47) in a randomized, controlled design with a three-year clinical intervention period and plasma samples were analyzed by using UPLC-LTQ-Orbitrap mass spectrometry...
December 2016: Aging and Disease
https://www.readbyqxmd.com/read/28042675/1%C3%A2-h-nmr-based-metabolomics-study-for-identifying-urinary-biomarkers-and-perturbed-metabolic-pathways-associated-with-severity-of-iga-nephropathy-a-pilot-study
#7
Shiva Kalantari, Mohsen Nafar, Shiva Samavat, Mahmoud Parvin
The severity of IgA nephropathy (IgAN), the most common primary glomerulonephritis, is judged on the basis of histologic and clinical features. A limited number of studies have considered molecular signature of IgAN for this issue and no reliable biomarkers have been presented non-invasively for use in patient evaluations. This study aims to identify metabolite markers excreted in the urine and impaired pathways that are associated with a known marker of severity (proteinuria) to predict mild and severe stages of IgAN...
January 1, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28032363/investigation-of-the-crosstalk-between-the-flg22-and-the-uv-b-induced-flavonol-pathway-in-arabidopsis-thaliana-seedlings
#8
Zheng Zhou, Dirk Schenke, Ying Miao, Daguang Cai
In Arabidopsis cell culture, we have shown that flavonol metabolite accumulation depends on expression of the flavonol pathway genes (FPGs), which are up-regulated by UV-B irradiation but repressed during microbe-associated molecular pattern (MAMP) -triggered immunity (MTI) induced by the bacterial elicitor flg22. The suppression of flavonoid production during MTI is believed to allow the plant focusing its metabolism on the pathogen defense by directing phenylalanine resources from UV-B protective flavonol production towards production of phytoalexins and cell wall fortification by lignin incorporation...
December 29, 2016: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28018418/plastoquinone-and-ubiquinone-in-plants-biosynthesis-physiological-function-and-metabolic-engineering
#9
REVIEW
Miaomiao Liu, Shanfa Lu
Plastoquinone (PQ) and ubiquinone (UQ) are two important prenylquinones, functioning as electron transporters in the electron transport chain of oxygenic photosynthesis and the aerobic respiratory chain, respectively, and play indispensable roles in plant growth and development through participating in the biosynthesis and metabolism of important chemical compounds, acting as antioxidants, being involved in plant response to stress, and regulating gene expression and cell signal transduction. UQ, particularly UQ10, has also been widely used in people's life...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28018404/functional-and-integrative-analysis-of-the-proteomic-profile-of-radish-root-under-pb-exposure
#10
Yan Wang, Liang Xu, Mingjia Tang, Haiyan Jiang, Wei Chen, Wei Zhang, Ronghua Wang, Liwang Liu
Lead (Pb) is one of the most abundant heavy metal (HM) pollutants, which can penetrate the plant through the root and then enter the food chain causing potential health risks for human beings. Radish is an important root vegetable crop worldwide. To investigate the mechanism underlying plant response to Pb stress in radish, the protein profile changes of radish roots respectively upon Pb(NO3)2 at 500 mg L(-1)(Pb500) and 1000 mg L(-1)(Pb1000), were comprehensively analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28018382/identification-and-analysis-of-nahco3-stress-responsive-genes-in-wild-soybean-glycine-soja-roots-by-rna-seq
#11
Jinlong Zhang, Jiaxue Wang, Wei Jiang, Juge Liu, Songnan Yang, Junyi Gai, Yan Li
Soil alkalinity is a major abiotic constraint to crop productivity and quality. Wild soybean (Glycine soja) is considered to be more stress-tolerant than cultivated soybean (G. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. In this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety N24852 at 12 and 24 h after 90 mM NaHCO3 stress by RNA-sequencing. Compared with the controls, a total of 449 differentially expressed genes (DEGs) were identified, including 95 and 140 up-regulated genes, and 108 and 135 down-regulated genes at 12 and 24 h after NaHCO3 treatment, respectively...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28008993/expanding-the-genetic-code-of-salmonella-with-non-canonical-amino-acids
#12
Qinglei Gan, Brent P Lehman, Thomas A Bobik, Chenguang Fan
The diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few studies on pathogens using genetic code expansion. Here, we introduce this technique into the human pathogen Salmonella by incorporating p-azido-phenylalanine, benzoyl-phenylalanine, acetyl-lysine, and phosphoserine into selected Salmonella proteins including a microcompartment shell protein (PduA), a type III secretion effector protein (SteA), and a metabolic enzyme (malate dehydrogenase), and demonstrate practical applications of genetic code expansion in protein labeling, photocrosslinking, and post-translational modification studies in Salmonella...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28007986/phospholipase-a2-activating-protein-is-associated-with-a-novel-form-of-leukoencephalopathy
#13
Tzipora C Falik Zaccai, David Savitzki, Yifat Zivony-Elboum, Thierry Vilboux, Eric C Fitts, Yishay Shoval, Limor Kalfon, Nadra Samra, Zohar Keren, Bella Gross, Natalia Chasnyk, Rachel Straussberg, James C Mullikin, Jamie K Teer, Dan Geiger, Daniel Kornitzer, Ora Bitterman-Deutsch, Abraham O Samson, Maki Wakamiya, Johnny W Peterson, Michelle L Kirtley, Iryna V Pinchuk, Wallace B Baze, William A Gahl, Robert Kleta, Yair Anikster, Ashok K Chopra
Leukoencephalopathies are a group of white matter disorders related to abnormal formation, maintenance, and turnover of myelin in the central nervous system. These disorders of the brain are categorized according to neuroradiological and pathophysiological criteria. Herein, we have identified a unique form of leukoencephalopathy in seven patients presenting at ages 2 to 4 months with progressive microcephaly, spastic quadriparesis, and global developmental delay. Clinical, metabolic, and imaging characterization of seven patients followed by homozygosity mapping and linkage analysis were performed...
December 21, 2016: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/28005947/metabolic-disturbances-in-adult-onset-still-s-disease-evaluated-using-liquid-chromatography-mass-spectrometry-based-metabolomic-analysis
#14
Der-Yuan Chen, Yi-Ming Chen, Han-Ju Chien, Chi-Chen Lin, Chia-Wei Hsieh, Hsin-Hua Chen, Wei-Ting Hung, Chien-Chen Lai
OBJECTIVE: Liquid chromatography/mass spectrometry (LC/MS)-based comprehensive analysis of metabolic profiles with metabolomics approach has potential diagnostic and predictive implications. However, no metabolomics data have been reported in adult-onset Still's disease (AOSD). This study investigated the metabolomic profiles in AOSD patients and examined their association with clinical characteristics and disease outcome. METHODS: Serum metabolite profiles were determined on 32 AOSD patients and 30 healthy controls (HC) using ultra-performance liquid chromatography (UPLC)/MS analysis, and the differentially expressed metabolites were quantified using multiple reactions monitoring (MRM)/MS analysis in 44 patients and 42 HC...
2016: PloS One
https://www.readbyqxmd.com/read/28000565/towards-tyrosine-metabolism-in-esophageal-squamous-cell-carcinoma
#15
Jing Cheng, Guangyong Zheng, Hai Jin, Xianfu Gao
BACKGROUND: Esophageal Squamous Cell Carcinoma (ESCC) is a common malignant tumor in China, which causes about 200,000 deaths each year. Sensitive biomarkers are helpful to diagnosis of the disease in early stage. METHOD: To identify biomarkers of ESCC and elucidate underlying mechanism of the disease, a targeted metabolomics strategy based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been implemented to explore tyrosine metabolism from 40 ESCC patients and 27 healthy controls...
December 20, 2016: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/27997218/inhibition-of-the-gut-enzyme-intestinal-alkaline-phosphatase-may-explain-how-aspartame-promotes-glucose-intolerance-and-obesity-in-mice
#16
Sarah S Gul, A Rebecca L Hamilton, Alexander R Munoz, Tanit Phupitakphol, Wei Liu, Sanjiv K Hyoju, Konstantinos P Economopoulos, Sara Morrison, Dong Hu, Weifeng Zhang, Mohammad Hadi Gharedaghi, Haizhong Huo, Sulaiman R Hamarneh, Richard A Hodin
Diet soda consumption has not been associated with tangible weight loss. Aspartame (ASP) commonly substitutes sugar and one of its breakdown products is phenylalanine (PHE), a known inhibitor of intestinal alkaline phosphatase (IAP), a gut enzyme shown to prevent metabolic syndrome in mice. We hypothesized that ASP consumption might contribute to the development of metabolic syndrome based on PHE's inhibition of endogenous IAP. The design of the study was such that for the in vitro model, IAP was added to diet and regular soda, and IAP activity was measured...
January 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/27988416/energy-metabolism-and-metabolomics-response-of-pacific-white-shrimp-litopenaeus-vannamei-to-sulfide-toxicity
#17
Tongyu Li, Erchao Li, Yantong Suo, Zhixin Xu, Yongyi Jia, Jian G Qin, Liqiao Chen, Zhimin Gu
The toxicity and poisoning mechanisms of sulfide were studied in Litopenaeus vannamei from the perspective of energy metabolism and metabolomics. The lethal concentrations of sulfide in L. vannamei (LC50) at 24h, 48h, 72h, and 96h were determined. Sulfide at a concentration of 0, 1/10 (425.5μg/L), and 1/5 (851μg/L) of the LC50 at 96h was used to test the metabolic responses of L. vannamei for 21days. The chronic exposure of shrimp to a higher sulfide concentration of 851μg/L decreased shrimp survival but did not affect weight gain or the hepatopancreas index...
December 12, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27977140/in-vivo-monitoring-of-amino-acid-biomarkers-from-inguinal-adipose-tissue-using-online-microdialysis-capillary-electrophoresis
#18
Megan M Weisenberger, Michael T Bowser
We have developed an online, high-throughput, microdialysis-capillary electrophoresis (MD-CE) assay for measuring the in vivo dynamics of amino acid biomarkers of metabolism in adipose tissue. Microdialysis probes were implanted into the inguinal adipose tissue depot of C57BL6 mice. The probe location and integrity were verified following each experiment, demonstrating our ability to accurately target the inguinal adipose tissue depot without damaging the probe. The relative concentrations of small molecule bioamines were measured in adipose tissue every 22 s...
December 15, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27941094/the-fusarium-crown-rot-pathogen-fusarium-pseudograminearum-triggers-a-suite-of-transcriptional-and-metabolic-changes-in-bread-wheat-triticum-aestivum-l
#19
Jonathan J Powell, Jason Carere, Timothy L Fitzgerald, Jiri Stiller, Lorenzo Covarelli, Qian Xu, Frank Gubler, Michelle L Colgrave, Donald M Gardiner, John M Manners, Robert J Henry, Kemal Kazan
BACKGROUND AND AIMS: Fusarium crown rot caused by the fungal pathogen Fusarium pseudograminearum is a disease of wheat and barley, bearing significant economic cost. Efforts to develop effective resistance to this disease have been hampered by the quantitative nature of resistance and a lack of understanding of the factors associated with resistance and susceptibility. Here, we aimed to dissect transcriptional responses triggered in wheat by F. pseudograminearum infection. METHODS: We used an RNA-seq approach to analyse host responses during a compatible interaction and identified >2700 wheat genes differentially regulated after inoculation with F...
December 10, 2016: Annals of Botany
https://www.readbyqxmd.com/read/27922243/late-diagnosed-phenylketonuria-in-an-eight-year-old-boy-with-dyslexia-and-attention-deficit-hyperactivity-disorder
#20
Yılmaz Yıldız, Ali Dursun, Ayşegül Tokatlı, Turgay Coşkun, Hatice Serap Sivri
Phenylketonuria, previously a common cause of severe intellectual disability, is a metabolic disorder now promptly diagnosed and effectively treated thanks to newborn screening programs. Here, we report a male patient presenting with dyslexia and attention-deficit hyperactivity disorder, who was diagnosed with mild phenylketonuria at eight years of age. Earlier recognition and treatment before the establishment of irreversible brain damage would have resulted in better neurobehavioural outcomes. Classical phenylketonuria and milder phenotypes of phenylalanine hydroxylase deficiency need to be considered in the differential diagnosis of all cognitive and behavioural problems of unknown cause...
2016: Turkish Journal of Pediatrics
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