keyword
MENU ▼
Read by QxMD icon Read
search

phenylalanine metabolism

keyword
https://www.readbyqxmd.com/read/29044214/limits-of-aerobic-metabolism-in-cancer-cells
#1
Jorge Fernandez-de-Cossio-Diaz, Alexei Vazquez
Cancer cells exhibit high rates of glycolysis and glutaminolysis. Glycolysis can provide energy and glutaminolysis can provide carbon for anaplerosis and reductive carboxylation to citrate. However, all these metabolic requirements could be in principle satisfied from glucose. Here we investigate why cancer cells do not satisfy their metabolic demands using aerobic biosynthesis from glucose. Based on the typical composition of a mammalian cell we quantify the energy demand and the OxPhos burden of cell biosynthesis from glucose...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29043820/stable-isotope-assisted-metabolic-profiling-reveals-growth-mode-dependent-differential-metabolism-and-multiple-catabolic-pathways-of-l-phenylalanine-in-rubrivivax-benzoatilyticus-ja2
#2
Lakshmi Prasuna Mekala, Mujahid Mohammed, Sasikala Chintalapati, Venakata Ramana Chintalapati
Anoxygenic phototrophic bacteria are metabolically versatile, survive under different growth modes using diverse organic compounds, yet their metabolic diversity is largely unexplored. In the present study, we employed stable isotope-assisted metabolic profiling to unravel the L-phenylalanine catabolism in Rubrivivax benzoatilyticus JA2 under varying growth modes. Strain JA2 grows under anaerobic and aerobic conditions by utilising L-phenylalanine as a nitrogen source. Further, ring labelled (13)C6-phenylalanine feeding followed by LC-MS exometabolite profiling revealed 60 labelled metabolic features(M+6/M+12/M+18) derived solely from L-phenylalanine, of which 11 were identified, 7 putatively identified and 42 unidentified under anaerobic, aerobic conditions...
October 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29037684/chitosan-delays-ripening-and-ros-production-in-guava-psidium-guajava-l-fruit
#3
Willian Batista Silva, Gláucia Michelle Cosme Silva, Diederson Bortolini Santana, Acácio Rodrigues Salvador, David Barbosa Medeiros, Ikram Belghith, Natália Martins da Silva, Maria Helena Menezes Cordeiro, Gisele Polete Misobutsi
Guava is a typically tropical fruit highly perishable with a short shelf-life due to intense metabolic activity after harvested. In attempt to minimize the problems related to the postharvest, we evaluated the physiochemical characteristics and antioxidant system in guava fruits under chitosan coating at concentrations of 1%, 2%, and 3% stored at 25°C during 96h. The chitosan suppressed the respiratory rate, fresh weight loss, firmness and skin color with delay in the degradation of chlorophyll. In the treatment with 2% and 3% of chitosan in the solid soluble content and ascorbic acid were reduced; retarded the loss of titratable acidity during 96h after treatment...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29032784/spent-culture-medium-analysis-from-individually-cultured-bovine-embryos-demonstrates-metabolomic-differences
#4
Kayla J Perkel, Pavneesh Madan
Spent culture medium can provide valuable information regarding the physiological state of a bovine preimplantation embryos through non-invasive analysis of the sum/depleted metabolite constituents. Metabolomics has become of great interest as an adjunct technique to morphological and cleavage-rate assessment, but more importantly, in improving our understanding of metabolism. In this study, in vitro produced bovine embryos developing at different rates were evaluated using proton nuclear magnetic resonance (1H NMR)...
October 16, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29032117/salvia-miltiorrhiza-protects-adenine-induced-chronic-renal-failure-by-regulating-the-metabolic-profiling-and-modulating-the-nadph-oxidase-ros-erk-and-tgf-%C3%AE-smad-signaling-pathways
#5
Hongdie Cai, Shulan Su, Yonghui Li, Huiting Zeng, Zhenhua Zhu, Jianming Guo, Yue Zhu, Sheng Guo, Li Yu, Dawei Qian, Yuping Tang, Jinao Duan
ETHNOPHARMACOLOGICAL RELEVANCE: Chronic renal failure (CRF) is defined as a progressive and irreversible loss of renal function and associated with inflammation and oxidative stress. Salvia miltiorrhiza (SM) is an important Chinese herb used in traditional Chinese medicine with notable effects on CRF. It significantly inhibited the elevation of serum creatinine (Scr), blood urea nitrogen (BUN) and urine protein (UP) in adenine-induced CRF rats, while the action mechanism need to be illuminated deeply...
October 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29025426/the-complete-european-guidelines-on-phenylketonuria-diagnosis-and-treatment
#6
REVIEW
A M J van Wegberg, A MacDonald, K Ahring, A Bélanger-Quintana, N Blau, A M Bosch, A Burlina, J Campistol, F Feillet, M Giżewska, S C Huijbregts, S Kearney, V Leuzzi, F Maillot, A C Muntau, M van Rijn, F Trefz, J H Walter, F J van Spronsen
Phenylketonuria (PKU) is an autosomal recessive inborn error of phenylalanine metabolism caused by deficiency in the enzyme phenylalanine hydroxylase that converts phenylalanine into tyrosine. If left untreated, PKU results in increased phenylalanine concentrations in blood and brain, which cause severe intellectual disability, epilepsy and behavioural problems. PKU management differs widely across Europe and therefore these guidelines have been developed aiming to optimize and standardize PKU care. Professionals from 10 different European countries developed the guidelines according to the AGREE (Appraisal of Guidelines for Research and Evaluation) method...
October 12, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/29021962/first-year-metabolic-control-guidelines-and-their-impact-on-future-metabolic-control-and-neurocognitive-functioning-in-children-with-pku
#7
Alicia de la Parra, María Ignacia García, Valerie Hamilton, Carolina Arias, Juan Francisco Cabello, Verónica Cornejo
There is a consensus on the importance of early and life-long treatment for PKU patients. Still, differences exist on target blood phenylalanine (Phe) concentrations for children with PKU in different countries and treatment centers. For the first time, long-term metabolic control and child development and cognitive functioning is compared between children with mean phenylalanine concentrations under 240 μmol/L (group A), between 240 and 360 μmol/L (group B) or over 360 μmol/L (group C) during their first year of life...
December 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28990194/relaxation-of-tyrosine-pathway-regulation-underlies-the-evolution-of-betalain-pigmentation-in-caryophyllales
#8
Samuel Lopez-Nieves, Ya Yang, Alfonso Timoneda, Minmin Wang, Tao Feng, Stephen A Smith, Samuel F Brockington, Hiroshi A Maeda
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic pathways. Betalain pigments, uniquely found in the plant order Caryophyllales, are synthesized from the aromatic amino acid l-tyrosine (Tyr) and replaced the otherwise ubiquitous phenylalanine-derived anthocyanins...
October 9, 2017: New Phytologist
https://www.readbyqxmd.com/read/28983702/measurement-of-the-15-n-14-n-ratio-of-phenylalanine-in-fermentation-matrix-by-isotope-ratio-mass-spectrometry
#9
Shiwei Zhang, Qiding Zhong, Daobing Wang, Zhanbin Huang, Guohui Li
OBJECTIVES: To determine the origin of (15)N-labeled phenylalanine in microbial metabolic flux analysis using (15)N as a tracer, a method for measuring phenylalanine δ(15)N using HPLC coupled with elemental analysis-isotope ratio mass spectrometry (EA-IRMS) was developed. RESULTS: The original source of the (15)N-labeled phenylalanine was determined using this new method that consists of three steps: optimization of the HPLC conditions, evaluation of the isotope fractionation effects, and evaluation of the effect of pre-processing on the phenylalanine nitrogen stable isotope...
October 5, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28982725/human-skeletal-muscle-is-refractory-to-the-anabolic-effects-of-leucine-during-the-postprandial-muscle-full-period-in-older-men
#10
W Kyle Mitchell, Bethan E Phillips, Ian Hill, Paul Greenhaff, Jonathan N Lund, John P Williams, Debbie Rankin, Daniel J Wilkinson, Kenneth Smith, Philip J Atherton
Leucine modulates muscle protein synthesis (MPS), with potential to facilitate accrual/maintenance of muscle mass. Animal models suggest leucine boluses shortly after meals may prolong MPS and delay onset of a "muscle-full" state. However, the effects of nutrient "top-ups" in humans, and particularly older adults where deficits exist, have not been explored. We determined the effects of a leucine top-up after essential amino acid (EAA) feeding on anabolic signaling, MPS and muscle energy metabolism in older men...
October 5, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28982351/spectrum-of-pah-gene-variants-among-a-population-of-han-chinese-patients-with-phenylketonuria-from-northern-china
#11
Ning Liu, Qiuying Huang, Qingge Li, Dehua Zhao, Xiaole Li, Lixia Cui, Ying Bai, Yin Feng, Xiangdong Kong
BACKGROUND: Phenylketonuria (PKU), which primarily results from a deficiency of phenylalanine hydroxylase (PAH), is one of the most common inherited inborn errors of metabolism that impairs postnatal cognitive development. The incidence of various PAH variations differs by race and ethnicity. The aim of the present study was to characterize the PAH gene variants of a Han population from Northern China. METHODS: In total, 655 PKU patients and their families were recruited for this study; each proband was diagnosed both clinically and biochemically with phenylketonuria...
October 5, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/28978542/consumption-of-whole-eggs-promotes-greater-stimulation-of-postexercise-muscle-protein-synthesis-than-consumption-of-isonitrogenous-amounts-of-egg-whites-in-young-men
#12
Stephan van Vliet, Evan L Shy, Sidney Abou Sawan, Joseph W Beals, Daniel Wd West, Sarah K Skinner, Alexander V Ulanov, Zhong Li, Scott A Paluska, Carl M Parsons, Daniel R Moore, Nicholas A Burd
Background: Protein in the diet is commonly ingested from whole foods that contain various macro- and micronutrients. However, the effect of consuming protein within its natural whole-food matrix on postprandial protein metabolism remains understudied in humans.Objective: We aimed to compare the whole-body and muscle protein metabolic responses after the consumption of whole eggs with egg whites during exercise recovery in young men.Design: In crossover trials, 10 resistance-trained men [aged 21 ± 1 y; 88 ± 3 kg; body fat: 16% ± 1% (means ± SEMs)] received primed continuous l-[ring-(2)H5]phenylalanine and l-[1-(13)C]leucine infusions and performed a single bout of resistance exercise...
October 4, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28977710/multifaceted-plant-reponses-to-circumvent-phe-hyperaccumulation-by-downregulation-of-flux-through-the-shikimate-pathway-and-by-vacuolar-phe-sequestration
#13
Joseph H Lynch, Irina Orlova, Chengsong Zhao, Longyun Guo, Rohit Jaini, Hiroshi Maeda, Tariq Akhtar, Junellie Cruz-Lebron, David Rhodes, John Morgan, Guillaume Pilot, Eran Pichersky, Natalia Dudareva
Detrimental effects of hyperaccumulation of the aromatic amino acid phenylalanine (Phe) in animals, known as phenylketonuria, are mitigated by excretion of Phe derivatives; however, how plants endure Phe accumulating conditions in the absence of excretion system is currently unknown. To achieve Phe hyperaccumulation in a plant system, we simultaneous decreased in petunia flowers expression of all three Phe ammonia lyase (PAL) isoforms that catalyze the non-oxidative deamination of Phe to trans-cinnamic acid, the committed step for the major pathway of Phe metabolism...
October 4, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28973422/elevated-metabolites-of-steroidogenesis-and-amino-acid-metabolism-in-preadolescent-female-children-with-high-urinary-bisphenol-a-levels-a-high-resolution-metabolomics-study
#14
Adnan Khan, Hyesook Park, Hye Ah Lee, Bohyun Park, Hye Sun Gwak, Hye-Ra Lee, Sun Ha Jee, Youngja H Park
Health risks associated with bisphenol A (BPA) exposure are controversially highlighted by numerous studies. High-resolution metabolomics can confirm these proposed associations and may provide a mechanistic insight into the connections between BPA exposure and metabolic perturbations. This study was aimed to identify the changes in metabolomics profile due to BPA exposure in urine and serum samples collected from female and male children (n = 18) aged 7-9. Urine was measured for BPA concentration, and the children were subsequently classified into high and low BPA groups...
September 9, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28970519/fungal-endophyte-induced-salidroside-and-tyrosol-biosynthesis-combined-with-signal-cross-talk-and-the-mechanism-of-enzyme-gene-expression-in-rhodiola-crenulata
#15
Jin-Long Cui, Ya-Nan Wang, Jin Jiao, Yi Gong, Jun-Hong Wang, Meng-Liang Wang
Endophyte is a factor that affects the physiology and metabolism of plant. However, limited information is available on the mechanism of interaction between endophyte and plant. To investigate the effects of endophytic fungus ZPRs-R11, that is, Trimmatostroma sp., on salidroside and tyrosol accumulations in Rhodiola crenulata, signal transduction, enzyme gene expression, and metabolic pathway were investigated. Results showed that hydrogen peroxide (H2O2), nitric oxide (NO), and salicylic acid (SA) involved in fungus-induced salidroside and tyrosol accumulations...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28970109/metabolic-profiling-of-garcinia-mangostana-mangosteen-based-on-ripening-stages
#16
Anjaritha A R Parijadi, Sastia P Putri, Sobir Ridwani, Fenny M Dwivany, Eiichiro Fukusaki
Metabolomics is an emerging research field based on exhaustive metabolite profiling that have been proven useful to facilitate the study of postharvest fruit development and ripening. Specifically, tracking changes to the metabolome as fruit ripens should provide important clues for understanding ripening mechanisms and identify bio-markers to improve post-harvest technology of fruits. This study conducted a time-course metabolome analysis in mangosteen, an economically important tropical fruit valued for its flavor...
September 29, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28969038/metabolomics-and-eicosanoid-analysis-identified-serum-biomarkers-for-distinguishing-hepatocellular-carcinoma-from-hepatitis-b-virus-related-cirrhosis
#17
Zhi-Gang Gong, Weijie Zhao, Jianbing Zhang, Xi Wu, Jing Hu, Guo-Chang Yin, Yong-Jiang Xu
Hepatocellular carcinoma (HCC) is one of the most common cancers in the world. It is a type of inflammation-related cancer that usually follows liver hepatitis that mostly caused by hepatitis B virus (HBV) in China. However, the metabolism disturbance of HCC and HBV-cirrhosis is not yet fully understood. In addition, there is little research on the relationships between inflammation mediators and HCC. In this study, we investigated serum metabolic abnormalities in HBV-cirrhosis and HCC patients through non-targeted metabolomics and targeted eicosanoid analysis...
September 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28965810/assessment-of-tryptophan-metabolism-and-signs-of-depression-in-individuals-with-carbohydrate-malabsorption
#18
Dietmar Enko, Helga Wagner, Gernot Kriegshäuser, Wolfgang Brandmayr, Gabriele Halwachs-Baumann, Wolfgang J Schnedl, Sieglinde Zelzer, Harald Mangge, Andreas Meinitzer
This prospective cross-sectional study aimed to investigate the potential association between primary-adult lactose malabsorption, fructose malabsorption, tryptophan (TRP) metabolism and the presence of depressive signs. Overall 251 patients, who were referred for lactase gene C/T-13910 polymorphism genotyping and fructose hydrogen/methane breath testing, were included. All participants filled out the Beck Depression Inventory (BDI II). Serum concentrations of tryptophan (TRP), kynurenine (KYN), kynuric acid (KYNA), and TRP competing amino acids (leucine, isoleucine, valine, phenylalanine, tyrosine) were measured by high-pressure liquid-chromatography...
September 22, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28965406/phenylalanine-increases-membrane-permeability
#19
Russell Perkins, Veronica Vaida
Biological membranes are a crucial aspect of living systems, controlling the organization and distribution of different chemical components. Control of membrane permeability is especially important for processes such as electron transport in metabolism and signal propagation in nerve cells. In this work, we show that the amino acid phenylalanine produces increased membrane permeability, which is likely responsible for some of the deleterious symptoms associated with high biological phenylalanine concentrations that occur with the genetic disorder phenylketonuria...
October 9, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28955992/postprandial-hepatic-protein-expression-in-trout-oncorhynchus-mykiss-a-proteomics-examination
#20
Eleni Mente, Graham J Pierce, Efthimia Antonopoulou, David Stead, Samuel A M Martin
Following a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, following a single meal to define the postprandial expression of hepatic proteins. The fish were fed a high marine fishmeal/fish oil single meal following a period of 24 h without feeding. Liver protein profiles were examined by 2D gel electrophoresis just before feeding (time 0 h) and at 6 and 12 h after feeding...
March 2017: Biochemistry and Biophysics Reports
keyword
keyword
64271
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"