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Han Liu, Chunlei Yu, Tianjiao Xu, Xiaojie Zhang, Miaoxian Dong
There are several factors, like oxidative stress and neurons loss, involving neurodegenerative diseases such as Parkinson's disease (PD). The combination of antioxidant and anti-apoptotic agent is becoming a promising approach to fight against PD. This study evaluates the hypothesis that paeoniflorin (PF) and β-ecdysterone (β-Ecd) synergize to protect PC12 cells against toxicity induced by PD-related neurotoxin rotenone. The combination of PF and β-Ecd, hereafter referred to as the PF/β-Ecd, at suboptimal concentrations increased the viability of rotenone-exposed PC12 cells in a synergistic manner...
September 29, 2016: Apoptosis: An International Journal on Programmed Cell Death
Athira Omanakuttan, Chinchu Bose, Nanjan Pandurangan, Geetha B Kumar, Asoke Banerji, Bipin G Nair
The complex process of wound healing is a major problem associated with diabetes, venous or arterial disease, old age and infection. A wide range of pharmacological effects including anabolic, anti-diabetic and hepato-protective activities have been attributed to Ecdysterone. In earlier studies, Ecdysterone has been shown to modulate eNOS and iNOS expression in diabetic animals and activate osteogenic differentiation through the Extracellular-signal-Regulated Kinase (ERK) pathway in periodontal ligament stem cells...
August 15, 2016: Experimental Cell Research
Nurmurod Sh Ramazanov, Isomiddin D Bobayev, Ugiloy Y Yusupova, Namuna K Aliyeva, Feruza R Egamova, Nigora Kh Yuldasheva, Vladimir N Syrov
A number of phytoecdysteroid compounds, such as ecdysterone, polipodin V, 2-deoxy-20-hydroxyecdysone, integristeron A and 2-deoxydizon were isolated from Stachys hissarica plant and their structures were confirmed by NMR, mass and IR spectroscopy. In addition, the biological activity of the S. hissarica plant's extract was tested on rats for wound healing activity. It was shown that the extract at repeated oral (per os) administration at a dose of 10 mg/kg speeds up the healing process of linear skin wounds in rats...
July 11, 2016: Natural Product Research
Chang-Wei Hsieh, Wen-Ching Ko, Wai-Jane Ho, Chao-Kai Chang, Guo-Jhou Chen, Jen-Chieh Tsai
OBJECTIVE: To investigate suitable condition for extraction of the active components from Ajuga nipponensis (A. nipponensis). METHODS: Orthogonal experimental design was used to determine the optimal extraction parameters for ecdysterones and flavonoids. Finally, the hepatoprotective abilities of A. nipponensis extracts were evaluated by CCl4-induced animal models. RESULTS: Maximum yields of flavonoids (7.87 ± 0.10) mg/g and ecdysterones (0...
May 2016: Asian Pacific Journal of Tropical Medicine
Wei Yin, Zu-rong Song, Jin-qi Liu, Guo-sheng Zhang
OBJECTIVE: To study the chemical constituents of Citrus medica fruit. METHODS: The fruit of Citrus medica was extracted with 95% EtOH, and the compounds were separated and purified by silica gel, RP-18 and Sephadex LH-20 column chromatography. The structures were identified by spectroscopic analysis. RESULTS: A total of 16 compounds were isolated and identified, including methyl ferulic acid (1), dihydro-N-caffeoyltyramine (2) acacetin (3), β-ecdysterone (4), (-)-balanophonin (5), p-methoxy cinnamic acid (6), umbelliferone (7), ferulic acid (8), pyrocatechualdehyde (9), diosmetin (10), 4-methoxy salicylic acid (1), β-amyrin acetate (12), epigallocatechin (13), betulinic acid (14), lupeol (15) and nicotinamide (16)...
October 2015: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
Zhi Pan, Yingcai Niu, Yini Liang, Xiaojie Zhang, Miaoxian Dong
Parkinson's disease (PD) is a neurological disorder pathologically characterized by loss of dopaminergic neurons in the substantia nigra. No curative therapy is available for PD. We recently found that phytoestrogen β-ecdysterone (β-Ecd) is able to reduce MPP(+)-induced apoptosis in PC12 cells. This study investigated the potential of β-Ecd to protect against SH-SY5Y cell apoptosis induced by the PD-related neurotoxin 6-hydroxydopamine (6-OHDA) and the underlying mechanism for this cytoprotection. In the present study, pretreatment with β-Ecd significantly reduced 6-OHDA-induced apoptosis of SH-SY5Y cells by a mitochondria-dependent pathway, as indicated by downregulation of Bax and PUMA (p53 upregulated modulator of apoptosis) expression, suppressing ΔΨm loss, inhibiting cytochrome c release, and attenuating caspase-9 activation...
October 2016: Neurotoxicity Research
Wen Sang, Lin Yu, Li He, Wei-Hua Ma, Zhi-Hui Zhu, Fen Zhu, Xiao-Ping Wang, Chao-Liang Lei
Ultraviolet B (UVB) radiation is an important environmental factor. It is generally known that UVB exhibits high genotoxicity due to causing DNA damage, potentially leading to skin carcinogenesis and aging in mammals. However, little is known about the effects of UVB on the development and metamorphosis of insects, which are the most abundant terrestrial animals. In the present study, we performed dose-response analyses of the effects UVB irradiation on Tribolium castaneum metamorphosis, assessed the function of the T...
2016: PloS One
Jai Gopal Sharma, Moirangthem Kameshwor Singh, Rina Chakrabarti
The antioxidant and growth stimulating properties of seeds of Achyranthes aspera were evaluated on UV-B irradiated Catla catla (catla) larvae. Catla larvae (initial weight: 1.2 ± 0.01 mg) were fed with four different diets--D1, D2 and D3 containing 0.1, 0.25 and 0.5% seeds of A. aspera and D4, control diet for 35 days. The larvae were then exposed to UV-B radiation (80 μW/cm2) on every alternate day for 20 days. Survival, growth, tissue glutamic oxaloacetic transminase (GOT), tissue glutamate pyruvate transminase (GPT), thiobarbituric acid reactive substances (TBARS) and nitric oxide synthase (NOS) were studied in larvae on day-21 of irradiation...
April 2015: Indian Journal of Biochemistry & Biophysics
M K Parr, F Botrè, A Naß, J Hengevoss, P Diel, G Wolber
Increasing numbers of dietary supplements with ecdysteroids are marketed as "natural anabolic agents". Results of recent studies suggested that their anabolic effect is mediated by estrogen receptor (ER) binding. Within this study the anabolic potency of ecdysterone was compared to well characterized anabolic substances. Effects on the fiber sizes of the soleus muscle in rats as well the diameter of C2C12 derived myotubes were used as biological readouts. Ecdysterone exhibited a strong hypertrophic effect on the fiber size of rat soleus muscle that was found even stronger compared to the test compounds metandienone (dianabol), estradienedione (trenbolox), and SARM S 1, all administered in the same dose (5 mg/kg body weight, for 21 days)...
June 2015: Biology of Sport
Yu Zou, Rongtian Wang, Hongyan Guo, Miaoxian Dong
Epidemiological studies have strongly linked postmenopausal estrogens uptake with a reduced risk of developing Parkinson's disease (PD) in women. Estrogen replacement therapy may be beneficial in early PD. The aim of this study is to evaluate the hypothesis that the neuroprotective effects of phytoestrogen β-ecdysterone (β-Ecd) might mainly result from its antioxidant capability by the activation of the phosphoinositide-3-kinase (PI3K)-nuclear factor E2-related factor 2 (Nrf2)-regulated signaling pathway...
September 2015: Toxicological Sciences: An Official Journal of the Society of Toxicology
Yi-Ni Luo, Hua Lin, Li-Wei Lin
OBJECTIVE: To study the microwave processing method of Achyranthis Bidentatae Radix processed with wine. METHODS: The content of total saponins, oleanolic acid and β-ecdysterone were determined as the indices to get the optimal microwave processing technology by orthogonal design. RESULTS: The best technology of Achyranthis Bidentatae Radix processed with wine were:the amount of vinegar was 20%, moistening time was 60 min, with 60% microwave heating for 3 min...
August 2014: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
Sara N Mitchell, Evdoxia G Kakani, Adam South, Paul I Howell, Robert M Waterhouse, Flaminia Catteruccia
The availability of genome sequences from 16 anopheline species provides unprecedented opportunities to study the evolution of reproductive traits relevant for malaria transmission. In Anopheles gambiae, a likely candidate for sexual selection is male 20-hydroxyecdysone (20E). Sexual transfer of this steroid hormone as part of a mating plug dramatically changes female physiological processes intimately tied to vectorial capacity. By combining phenotypic studies with ancestral state reconstructions and phylogenetic analyses, we show that mating plug transfer and male 20E synthesis are both derived characters that have coevolved in anophelines, driving the adaptation of a female 20E-interacting protein that promotes oogenesis via mechanisms also favoring Plasmodium survival...
February 27, 2015: Science
Suzanne H Alonzo
No abstract text is available yet for this article.
February 27, 2015: Science
R R Sharipov, A V Kotsiuruba, B S Kop"iak, V F Sahach
On the model of focal ischemia-reperfusion of the brain investigated the induction of nitrosative stress in mitochondria of rats hearts and possible mechanisms of protective action of ecdysterone. It is shown that focal ischemia-reperfusion of the brain induced in myocardial mitochondria the activation of constitutive and inducible de novo synthesis of NO by oxidation of L-arginine and not oxidative synthesis of NO through the recovery of oxidized stable metabolites of NO. Strong evidence of induction of nitrosative stress in heart mitochondria by focal ischemia-reperfusion of the brain, was a significant increase in mitochondrial pool of nitrate- and nitrite-anions and pools of nitrosothiols, that is proof of the formation and decay of peroxynitrite--a key marker of nitrosative stress...
2014: Fiziolohichnyĭ Zhurnal
Paolo Gabrieli, Evdoxia G Kakani, Sara N Mitchell, Enzo Mameli, Elizabeth J Want, Ainhoa Mariezcurrena Anton, Aurelio Serrao, Francesco Baldini, Flaminia Catteruccia
Female insects generally mate multiple times during their lives. A notable exception is the female malaria mosquito Anopheles gambiae, which after sex loses her susceptibility to further copulation. Sex in this species also renders females competent to lay eggs developed after blood feeding. Despite intense research efforts, the identity of the molecular triggers that cause the postmating switch in females, inducing a permanent refractoriness to further mating and triggering egg-laying, remains elusive. Here we show that the male-transferred steroid hormone 20-hydroxyecdysone (20E) is a key regulator of monandry and oviposition in An...
November 18, 2014: Proceedings of the National Academy of Sciences of the United States of America
Panlin Li, Weiwei Su, Chengshi Xie, Xuan Zeng, Wei Peng, Menghua Liu
A rapid and high-sensitive ultra-fast liquid chromatography coupled with a diode-array detector and a quadrupole/time-of-flight mass spectrometry (MS) method was established and validated for the chemical profiling of Nao-shuan-tong capsule (NSTC) and simultaneous quantification of five major constituents. A total of 59 components including monoterpene glycosides, flavonoids, sesquiterpenoids, ketosteroids, thiophenes, organic acids and alkaloids were identified or tentatively characterized in NSTC based on the accurate mass and tandem MS behavior...
July 2015: Journal of Chromatographic Science
V N Syrov, Zh I Islamova, F R Égamova, N Kh Iuldasheva, Z A Khushbaktova
Introduction of phytoecdysteroids (ecdysterone, turkesterone and ñyasterone) to rats subjected to prolonged immobilization stress significantly decreased involution of the thymus and spleen, contributed to normalization of increased mass of adrenal glands, and restored their content of ascorbic acid and cholesterol. In the liver of stressed animals, phytoecdysteroids prevented sharp decrease in the glycogen concentration, showed a clear trend toward normalization of the ratio of lactic and pyruvic acids, maintained homeostasis of macroergic phosphorus compounds, and increased the activity of antioxidant enzymes that inhibit lipid peroxidation...
2014: Eksperimental'naia i Klinicheskaia Farmakologiia
Fanchi Li, Zhiya Gu, Binbin Wang, Yi Xie, Lie Ma, Kaizun Xu, Min Ni, Hua Zhang, Weide Shen, Bing Li
Silkworm (Bombyx mori), a model Lepidoptera insect, is economically important. Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applications using metallic nanoparticles has increased the possibility of human or ecosystem exposure due to their unintentional release into the environment. We investigated the effects of exposure to titanium dioxide nanoparticles (TiO2 NPs) on the action of 20E in B...
August 2014: Journal of Chemical Ecology
Jing Ren, Xiang-Ru Li, Peng-Cheng Liu, Mei-Juan Cai, Wen Liu, Jin-Xing Wang, Xiao-Fan Zhao
The nuclear receptor-mediated genomic pathways of the animal steroid hormones are well known. However, the cell membrane receptor-mediated nongenomic pathways of the animal steroid hormones are little understood. In this study, we report the participation of a G-protein alpha q (Gαq)(1) subunit in the 20E nongenomic pathway in the cell membrane and regulating gene expression during molting and metamorphosis in a lepidopteran insect, Helicoverpa armigera. 20E-induced phosphorylation of Gαq was detected using two-dimensional electrophoresis techniques...
October 2014: Journal of Steroid Biochemistry and Molecular Biology
Mei-Juan Cai, Wen Liu, Xu-Yang Pei, Xiang-Ru Li, Hong-Juan He, Jin-Xing Wang, Xiao-Fan Zhao
The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis. By contrast, juvenile hormone (JH) prevents metamorphosis. However, the mechanism by which JH inhibits metamorphosis remains unclear. In this study, we propose that JH induces the phosphorylation of Broad isoform Z7 (BrZ7), a newly identified protein, to inhibit 20E-mediated metamorphosis in the lepidopteran insect Helicoverpa armigera. The knockdown of BrZ7 in larvae inhibited metamorphosis by repressing the expression of the 20E response gene...
September 19, 2014: Journal of Biological Chemistry
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