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14-3-3 protein

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https://www.readbyqxmd.com/read/28222366/alterations-of-tissue-metallothionein-and-vitellogenin-concentrations-in-tropical-cup-oysters-saccostrea-sp-following-short-term-96h-exposure-to-cadmium
#1
Angela M Moncaleano-Niño, Sergio A Barrios-Latorre, Javier F Poloche-Hernández, Vanessa Becquet, Valérie Huet, Luisa Villamil, Hélène Thomas-Guyon, Michael J Ahrens, Andrea Luna-Acosta
Metallothioneins and vitellogenins are low molecular weight proteins that have been used widely in environmental monitoring as biomarkers of exposure and damage to metals and estrogenic compounds, respectively. In the present study, the responses of metallothionein and vitellogenin tissue concentrations were measured following acute (96h) aqueous exposures to cadmium in Saccostrea sp., a tropical cup oyster native to the Western Pacific Ocean that has recently established itself in the Caribbean Sea. Adult oysters (1...
February 9, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28222207/impact-of-the-type-of-dialysis-membranes-on-the-circulating-concentration-of-markers-of-vitamin-d-metabolism
#2
Etienne Cavalier, Pablo U Torres, Bernard E Dubois, Nicole Smelten, Hans Pottel, Jean-Marie Krzesinski, Pierre Delanaye
INTRODUCTION: Kidney Disease Improving Global Outcomes (KDIGO) guidelines recommend vitamin D supplementation in hemodialyzed patients to monitor 25(OH)-vitamin D 25(OH)D levels. However, patient-to-patient inconsistency can be observed in response to the treatment. In this study, we aimed to evaluate the impact of the dialysis membrane on 25(OH)D, albumin (Alb) and vitamin D-binding protein (VDBP), the major players of vitamin D transport and storage. MATERIAL AND METHODS: Alb (Cobas), VDBP (R&D) and 25(OH)D (liquid chromatography-tandem mass spectrometry) were measured in 75 patients before and after a 4-hour dialysis session...
February 18, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28221044/tunable-volumetric-density-and-porous-structure-of-spherical-poly-%C3%A9-caprolactone-microcarriers-as-applied-in-human-mesenchymal-stem-cell-expansion
#3
Jian Li, Alan Tin-Lun Lam, Jessica Pei Wen Toh, Shaul Reuveny, Steve Kah-Weng Oh, William R Birch
Polymeric microspheres may serve as microcarrier matrices, for the expansion of anchorage-dependent stem cells. They require surface properties that both promote initial cell adhesion and the subsequent spreading of cells, which is a prerequisite for successful expansion. When implemented in a three-dimensional culture environment, under agitation, their suspension under low shear rates depends on the microcarriers having a modest negative buoyancy, with a density of 1.02-1.05 g/cm(3). Bioresorbable polycaprolactone (PCL), with a density of 1...
February 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28220305/homeobox-wnt-and-fibroblast-growth-factor-signaling-is-augmented-during-alveogenesis-in-mice-lacking-superoxide-dismutase-3-extracellular
#4
Tania A Thimraj, Rahel L Birru, Ankita Mitra, Holger Schulz, George D Leikauf, Koustav Ganguly
Superoxide dismutase 3, extracellular (SOD3) polymorphisms have been implicated in reduced pulmonary function development and altered risk for chronic obstructive pulmonary disease. We previously reported that gene-targeted Sod3-/- mice have impaired lung function and human SOD3 variants are associated with reduced pulmonary function in children. Reduced lung SOD3 levels were reported in mice with lower lung function with the greatest difference occurring during alveogenesis phase [postnatal (P) days 14-28]...
February 20, 2017: Lung
https://www.readbyqxmd.com/read/28219739/gapa-a-potential-vaccine-candidate-antigen-against-streptococcus-agalactiae-in-nile-tilapia-oreochromis-niloticus
#5
Ze Zhang, Angen Yu, Jiangfeng Lan, Hua Zhang, Minqiang Hu, Jiewei Cheng, Lijuan Zhao, Li Lin, Shun Wei
Streptococcosis due to the bacterium Streptococcus agalactiae (S. agalactiae) has resulted in enormous economic losses in aquaculture worldwide, especially in the tilapia culture industry. Previously, there were limited vaccines that could be employed against streptococcosis in tilapia. This study aimed to develop a vaccine candidate using the glyceraldehyde-phosphate dehydrogenase protein (GapA) of S. agalactiae encoded by the gapA gene. Tilapia were intraperitoneally injected with PBS, PBS + Freund's adjuvant, PBS + Montanide's adjuvant, GapA + Freund's adjuvant, GapA + Montanide's adjuvant, killed S...
February 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28219465/relationships-between-methane-emission-of-holstein-friesian-dairy-cows-and-fatty-acids-volatile-metabolites-and-non-volatile-metabolites-in-milk
#6
S van Gastelen, E C Antunes-Fernandes, K A Hettinga, J Dijkstra
This study investigated the relationships between methane (CH4) emission and fatty acids, volatile metabolites (V) and non-volatile metabolites (NV) in milk of dairy cows. Data from an experiment with 32 multiparous dairy cows and four diets were used. All diets had a roughage : concentrate ratio of 80 : 20 based on dry matter (DM). Roughage consisted of either 1000 g/kg DM grass silage (GS), 1000 g/kg DM maize silage (MS), or a mixture of both silages (667 g/kg DM GS and 333 g/kg DM MS; 333 g/kg DM GS and 677 g/kg DM MS)...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28219345/biomarkers-for-the-detection-of-renal-fibrosis-and-prediction-of-renal-outcomes-a-systematic-review
#7
Sherry G Mansour, Jeremy Puthumana, Steven G Coca, Mark Gentry, Chirag R Parikh
BACKGROUND: Fibrosis is the unifying pathway leading to chronic kidney disease. Identifying biomarkers of fibrosis may help predict disease progression. METHODS: We performed a systematic review to evaluate the reliability of blood and urine biomarkers in identifying fibrosis on biopsy as well as predicting renal outcomes. Using MEDLINE and EMBASE, a two-stage search strategy was implemented. Stage I identified a library of biomarkers correlating with fibrosis on biopsy...
February 20, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28216408/neuroprotective-property-of-low-molecular-weight-fraction-from-b-jararaca-snake-venom-in-h2o2-induced-cytotoxicity-in-cultured-hippocampal-cells
#8
Samyr Machado Querobino, Daniel Carneiro Carrettiero, Maricilia Silva Costa, Carlos Alberto-Silva
In central nervous system cells, low molecular weight fractions (LMWF) from snake venoms can inhibit changes in mitochondrial membrane permeability, preventing the diffusion of cytochrome c to the cytoplasm, inhibiting the activation of pro-apoptotic factors. Here, we evaluated the neuroprotective activity of LMWF from Bothrops jararaca (Bj) snake venom in H2O2-induced cytotoxicity in cultured hippocampal cells. SDS-PAGE, FT-IR and MALDI-TOF analysis of LMWF (<14 kDa) confirmed the absence of high-molecular-weight proteins in the fraction...
February 16, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28215987/accelerated-retinal-aging-in-pacap-knock-out-mice
#9
Andrea Kovács-Valasek, Krisztina Szabadfi, Viktória Dénes, Bálint Szalontai, Andrea Tamás, Péter Kiss, Aliz Szabó, Gyorgy Setalo, Dóra Reglődi, Robert Gábriel
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurotrophic and neuroprotective peptide. PACAP and its receptors are widely distributed in the retina. A number of reports provided evidence that PACAP is neuroprotective in retinal degenerations. The current study compared retina cell type-specific differences in young (3-4months) and aged adults (14-16months), of wild-type (WT) mice and knock-out (KO) mice lacking endogenous PACAP production during the course of aging. Histological, immunocytochemical and Western blot examinations were performed...
February 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28215864/regulatory-effects-of-echinococcus-multilocularis-extracellular-vesicles-on-raw264-7-macrophages
#10
Yadong Zheng, Xiaola Guo, Meng Su, Aijiang Guo, Juntao Ding, Jing Yang, Haitao Xiang, Xiaoan Cao, Shaohua Zhang, Mazhar Ayaz, Xuenong Luo
Extracellular vesicles (EVs) play a role in intercellular communications via exchanging biological molecules, being involved in host-parasite interplay. Little is to date known about E. multilocularis EVs and their biological activities. Here spherical EVs secreted by E. multilocularis metacestodes were shown to range predominately from 34nm to 95nm in diameter. A total of 433 proteins were identified in the EVs, and the proteins involved in binding (42%) and catalytic activity (41%) were most frequently represented...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215185/nutritive-value-and-anthelmintic-effect-of-sainfoin-pellets-fed-to-experimentally-infected-growing-rabbits
#11
H Legendre, H Hoste, T Gidenne
Alternative strategies to synthetic chemical drugs are needed in livestock and are a key issue in organic farming today. This study aimed at examining the potentialities of sainfoin, a legume rich in condensed tannins, as a nutraceutical that combines nutritive and antiparasitic effects in rabbits. To test the effect of infection with a helminth (I: infected groups; NI: not infected groups) and the effect of substituting 40% of the alfalfa in a control diet (C) with sainfoin (diet S), four groups of 16 weaned rabbits were arranged according to a 2×2 bifactorial design...
February 20, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28215140/position-based-nucleotide-analysis-of-mir168-family-in-higher-plants-and-its-targets-in-mammalian-transcripts
#12
Mohammed Javed, Manish Solanki, Anshika Sinha, Lata Israni Shukla
The conserved miR168 family is evaluated for position based nucleotide preference in higher plants. The mature miRNA sequences include miR168-5p and miR168-3p, were obtained from miRBase (v21, June 2014) for 15 families (28 plants) containing a-c subfamilies. The preferred position based nucleotide sequences were obtained for miR168-5p and miR168-3p using Data Analysis in Molecular Biology and Evolution (DAMBE). miR168-5p shows same nucleotides at positions 1-6, 8-9, 11-12, 15-17 and 19. Also, miR168-3p is present in 3 families (10 plants) shows the same nucleotide at position 1-11, 13-15 and 17-21...
February 15, 2017: MicroRNA
https://www.readbyqxmd.com/read/28214838/the-role-of-hippocampal-structural-synaptic-plasticity-in-repetitive-transcranial-magnetic-stimulation-to-improve-cognitive-function-in-male-samp8-mice
#13
Jiang Ma, Jinhua Wang, Chaonan Lv, Jingjuan Pang, Bing Han, Mingwei Wang, Yuan Gen
BACKGROUND: Repetitive transcranial magnetic stimulation (rTMS) has been used to improve cognitive function, but the stimulation protocols are variable and the underlying mechanism is unclear. Therefore, we intend to examine whether 5Hz rTMS with 30% maximum output could improve cognitive functions in senescence-accelerated-prone mouse 8 (SAMP8) through changing synaptic plasticity. METHODS: SAMP8 and senescence-accelerated-prone mouse/resistant 1 (SAMR1) (7-month old male) were randomly divided into 3 groups: SMAP8 rTMS group (P8-rTMS), SMAP8 sham-rTMS group (P8-sham), and SAMR1 sham-rTMS group (R1-sham)...
January 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214040/reinforcement-of-intestinal-epithelial-barrier-by-arabinoxylans-in-overweight-and-obese-subjects-a-randomized-controlled-trial-arabinoxylans-in-gut-barrier
#14
Bouke N Salden, Freddy J Troost, Ellen Wilms, Pilar Truchado, Ramiro Vilchez-Vargas, Dietmar H Pieper, Ruy Jáuregui, Massimo Marzorati, Tom van de Wiele, Sam Possemiers, Ad A Masclee
BACKGROUND & AIMS: Obesity and metabolic diseases are associated with alterations in microbial composition and impaired gut barrier. Previous in vitro and animal studies have shown that arabinoxylans (AX) have the potential to modulate gut microbiota and gut barrier and therefore could have a protective role. Primary aim of the study was to investigate the effect of AX on intestinal permeability. Secondary aims included the effect of AX on gene transcription and protein expression of tight junctions (TJ), intestinal microbiota composition and activity, immune response and metabolic markers in overweight and obese individuals...
February 3, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28213566/a-randomised-phase-iib-study-of-mavrilimumab-a-novel-gm-csf-receptor-alpha-monoclonal-antibody-in-the-treatment-of-rheumatoid-arthritis
#15
Gerd R Burmester, Iain B McInnes, Joel Kremer, Pedro Miranda, Mariusz Korkosz, Jiri Vencovsky, Andrea Rubbert-Roth, Eduardo Mysler, Matthew A Sleeman, Alex Godwood, Dominic Sinibaldi, Xiang Guo, Wendy I White, Bing Wang, Chi-Yuan Wu, Patricia C Ryan, David Close, Michael E Weinblatt
OBJECTIVES: Despite the therapeutic value of current rheumatoid arthritis (RA) treatments, agents with alternative modes of action are required. Mavrilimumab, a fully human monoclonal antibody targeting the granulocyte-macrophage colony-stimulating factor receptor-α, was evaluated in patients with moderate-to-severe RA. METHODS: In a phase IIb study (NCT01706926), patients with inadequate response to ≥1 synthetic disease-modifying antirheumatic drug(s), Disease Activity Score 28 (DAS28)-C reactive protein (CRP)/erythrocyte sedimentation rate ≥3...
February 17, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28213564/seven-year-tolerability-profile-of-glucocorticoids-use-in-early-rheumatoid-arthritis-data-from-the-espoir-cohort
#16
Camille Roubille, Nathalie Rincheval, Maxime Dougados, René-Marc Flipo, Jean-Pierre Daurès, Bernard Combe
OBJECTIVE: To explore the 7-year tolerability profile of glucocorticoids (GC) for early rheumatoid arthritis (RA). METHODS: We examined data for 602 patients with RA from the early arthritis Etude et Suivi des POlyarthrites Indifférenciées Récentes (ESPOIR) cohort (<6 months disease duration) stratified into two groups: with or without GC treatment at least once during follow-up (median 7 years (IQR 0.038-7.65)). The main outcome was a composite of death, cardiovascular disease (including myocardial ischaemia, cerebrovascular accident and heart failure), severe infection and fracture...
February 17, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28212898/low-serum-iron-is-associated-with-high-serum-intact-fgf23-in-elderly-men-the-swedish-mros-study
#17
Catharina Lewerin, Östen Ljunggren, Herman Nilsson-Ehle, Magnus K Karlsson, Hans Herlitz, Mattias Lorentzon, Claes Ohlsson, Dan Mellström
BACKGROUND: Fibroblast growth factor (FGF23) is a protein that is produced by osteoblasts and osteocytes. Increased serum levels of FGF23 have been associated with increased risks of osteoporotic fractures and cardiovascular disease, particularly in participants with poor renal function. Serum iron (Fe) has been suggested as a regulator of FGF23 homeostasis. OBJECTIVE: To determine whether Fe and iron status are determinants of the levels of intact FGF23 (iFGF23) in elderly men...
February 14, 2017: Bone
https://www.readbyqxmd.com/read/28210969/host-tissue-response-by-the-expression-of-collagen-to-cyanoacrylate-adhesives-used-in-implant-fixation-for-abdominal-hernia-repair
#18
Gemma Pascual, Marta Rodríguez, Bárbara Pérez-Köhler, Claudia Mesa-Ciller, Mar Fernández-Gutiérrez, Julio San Román, Juan M Bellón
The less traumatic use of surgical adhesives rather than sutures for mesh fixation in hernia repair has started to gain popularity because they induce less host tissue damage and provoke less postoperative pain. This study examines the host tissue response to a new cyanoacrylate (CA) adhesive (n-octyl, OCA). Partial defects (3 × 5 cm) created in the rabbit anterior abdominal wall were repaired by mesh fixation using OCA, Glubran2(®)(n-butyl-CA), Ifabond(®)(n-hexyl-CA) or sutures. Samples were obtained at 14/90 days for morphology, collagens qRT-PCR/immunofluorescence and biomechanical studies...
April 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28210225/increased-apoptosis-in-the-paraventricular-nucleus-mediated-by-at1r-ras-erk1-2-signaling-results-in-sympathetic-hyperactivity-and-renovascular-hypertension-in-rats-after-kidney-injury
#19
Hongguo Zhu, Lishan Tan, Yumin Li, Jiawen Li, Minzi Qiu, Lanying Li, Mengbi Zhang, Min Liang, Aiqing Li
Background: The central nervous system plays a vital role in the development of hypertension, but the molecular regulatory mechanisms are not fully understood. This study aimed to explore signaling in the paraventricular nucleus (PVN) which might contribute to renal hypertension. Methods: Renal hypertension model was established by five-sixth nephrectomy operation (5/6Nx) in male Sprague Dawley rats. Ten weeks afterwards, they were random assigned to no treatment, or intracerebroventricular injection (ICV) with artificial cerebrospinal fluid, losartan [angiotensin II receptor type 1 (AT1R) antagonist], farnesylthiosalicylic acid (Ras inhibitor), PD98059 (MEK inhibitor), or SB203580 (p38 inhibitor) and Z-DEVD-FMK (caspase-3 inhibitor)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28209772/maternal-smoke-exposure-decreases-mesenchymal-proliferation-and-modulates-rho-gtpase-dependent-actin-cytoskeletal-signaling-in-fetal-lungs
#20
Uchenna Unachukwu, Jordis Trischler, Monica Goldklang, Rui Xiao, Jeanine D'Armiento
The present study tested the hypothesis that maternal smoke exposure results in fetal lung growth retardation due to dysregulation in various signaling pathways, including the Wnt (wingless-related integration site)/β-catenin pathway. Pregnant female C57BL/6J mice were exposed to cigarette smoke (100-150 mg/m(3)) or room air, and offspring were humanely killed on 12.5, 14.5, 16.5, and 18.5 d post coitum (dpc). We assessed lung stereology with Cavalieri estimation; apoptosis with proliferating cell nuclear antigen, TUNEL, and caspase assays; and gene expression with quantitative PCR (qPCR) and RNA sequencing on lung epithelium and mesenchyme retrieved by laser capture microdissection...
February 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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