Read by QxMD icon Read


Virág Bogdándi, Tomoaki Ida, Thomas R Sutton, Christopher Bianco, Tamás Ditrói, Grielof Koster, Hillary A Henthorn, Magda Minnion, John P Toscano, Albert van der Vliet, Michael D Pluth, Martin Feelisch, Jon M Fukuto, Takaaki Akaike, Péter Nagy
BACKGROUND AND PURPOSE: Posttranslational modifications of cysteine (Cys) residues represent a major aspect of redox biology, and their reliable detection is key in providing mechanistic insights. The metastable character of these modifications and cell lysis-induced artifactual oxidation render current state-of-the-art protocols to rely on alkylation-based stabilization of labile Cys derivatives before cell/tissue rupture. An untested assumption in these procedures is that for all Cys derivatives alkylation rates are faster than their dynamic interchange...
June 17, 2018: British Journal of Pharmacology
Xinying Li
Mechanisms of Ag-X and Ag…H2 interactions and stabilities of T-shaped H2 -AgX (X = F - I) series were investigated at the CCSD(T) level. The "no-density" bond with smaller positive Laplacian and ELF values in interaction regions, as well as considerable delocalization index values, suggest weak covalent "charge-shift" character for the Ag…H2 interaction. Structure and stability dependence on the electric field were investigated at the MP2 level. Relative total energy curves show obvious parabolic character, and the plots can be fitted by quadratic polynomials as functions of electric field strength...
June 16, 2018: Journal of Molecular Modeling
Linling Li, Erni Ji, Fei Tang, Yunhai Qiu, Xue Han, Shengli Zhang, Zhiguo Zhang, Haichen Yang
Numerous functional magnetic resonance imaging studies have been conducted to elucidate emotion processing of patients with bipolar disorder (BD), but due to different inclusion criteria used, especially for the history of medication use, the results for euthymic BD patients are inconsistent. For this reason, brain functional effects of psychopharmacological treatments on BD patients have been investigated by numerous fMRI studies, but there is no existing report for brain functional effects of different mood stabilizers...
June 16, 2018: Brain Imaging and Behavior
Sebastián Cerminati, Luciana Paoletti, Salvador Peirú, Hugo G Menzella, María Eugenia Castelli
βγ-crystallin has emerged as a superfamily of structurally homologous proteins with representatives across all domains of life. A major portion of this superfamily is constituted by microbial members. This superfamily has also been recognized as a novel group of Ca2+ -binding proteins with a large diversity and variable properties in Ca2+ binding and stability. We have recently described a new phosphatidylinositol phospholipase C from Lysinibacillus sphaericus (LS-PIPLC) which was shown to efficiently remove phosphatidylinositol from crude vegetable oil...
June 16, 2018: Applied Microbiology and Biotechnology
Skylar R Hopkins, Cari M McGregor, Lisa K Belden, Jeremy M Wojdak
All dynamic species interaction models contain an assumption that describes how contact rates scale with population density. Choosing an appropriate contact-density function is important, because different functions have different implications for population dynamics and stability. However, this choice can be challenging, because there are many possible functions, and most are phenomenological and thus difficult to relate to underlying ecological processes. Using one such phenomenological function, we described a nonlinear relationship between field transmission rates and host density in a common snail-oligochaete symbiosis...
June 16, 2018: Oecologia
Asma'a A Al-Ekrish, Sara A Alfadda, Wadea Ameen, Romed Hörmann, Wolfgang Puelacher, Gerlig Widmann
PURPOSE: To compare the surface of computer-aided design (CAD) models of the maxilla produced using ultra-low MDCT doses combined with filtered backprojection (FBP), adaptive statistical iterative reconstruction (ASIR) and model-based iterative reconstruction (MBIR) reconstruction techniques with that produced from a standard dose/FBP protocol. METHODS: A cadaveric completely edentulous maxilla was imaged using a standard dose protocol (CTDIvol: 29.4 mGy) and FBP, in addition to 5 low dose test protocols (LD1-5) (CTDIvol: 4...
June 16, 2018: International Journal of Computer Assisted Radiology and Surgery
Ksenia Derina, Elena Korotkova, Yekaterina Taishibekova, Lyazat Salkeeva, Bohumil Kratochvil, Jiri Barek
The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier...
June 16, 2018: Analytical and Bioanalytical Chemistry
Liping Zhao, Juan Qiao, Meyong Hee Moon, Li Qi
Fabrication of polymer membranes with nanopores and a confinement effect toward enzyme immobilization has been an enabling endeavor. In the work reported here, an enzyme reactor based on a thermoresponsive magnetic porous block copolymer membrane was designed and constructed. Reversible addition-fragmentation chain transfer polymerization was used to synthesize the block copolymer, poly(maleic anhydride-styrene-N-isopropylacrylamide), with poly(N-isopropylacrylamide) as the thermoresponsive moiety. The self-assembly property of the block copolymer was used for preparation of magnetic porous thin film matrices with iron oxide nanoparticles...
June 16, 2018: Analytical and Bioanalytical Chemistry
Archana Shubhakar, Poh-Choo Pang, Daryl L Fernandes, Anne Dell, Daniel I R Spencer, Stuart M Haslam
Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins. Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life. One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS). The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps...
June 16, 2018: Glycoconjugate Journal
Audrey Hammack, Rashed T Rihani, Bryan J Black, Joseph J Pancrazio, Bruce E Gnade
Substrate-integrated microelectrode arrays (MEAs) are non-invasive platforms for recording supra-threshold signals, i.e. action potentials or spikes, from a variety of cultured electrically active cells, and are useful for pharmacological and toxicological studies. However, the MEA substrate, which is often fabricated using semiconductor processing technology, presents some challenges to the user. Specifically, the electrode encapsulation, which may consist of a variety of inorganic and organic materials, requires a specific substrate preparation protocol to optimize cell adhesion to the surface...
June 16, 2018: Biomedical Microdevices
Melissa S Standley, Samuel Million-Weaver, David L Alexander, Shuai Hu, Manel Camps
ColE1-like plasmid vectors are widely used for expression of recombinant genes in E. coli. For these vectors, segregation of individual plasmids into daughter cells during cell division appears to be random, making them susceptible to loss over time when no mechanisms ensuring their maintenance are present. Here we use the plasmid pGFPuv in a recA relA strain as a sensitized model to study factors affecting plasmid stability in the context of recombinant gene expression. We find that in this model, plasmid stability can be restored by two types of genetic modifications to the plasmid origin of replication (ori) sequence: point mutations and a novel 269 nt duplication at the 5' end of the plasmid ori, which we named DAS (duplicated anti-sense) ori...
June 16, 2018: Current Genetics
Zishuo Cheng, Pei W Thomas, Lincheng Ju, Alexander Bergstrom, Kelly Mason, Delaney Clayton, Callie Miller, Christopher R Bethel, Jamie VanPelt, David L Tierney, Richard C Page, Robert A Bonomo, Walter Fast, Michael W Crowder
Infections by carbapenem-resistant Enterobacteriaceae (CRE) are difficult to manage owing to broad antibiotic resistance profiles and because of the inability of clinically-used β-lactamase inhibitors to counter the activity of metallo-β-lactamases often harbored by these pathogens. Of particular importance is New Delhi metallo-β-lactamase (NDM), which requires a dinuclear zinc ion cluster for catalytic activity. Here, we compare the structures and functions of clinical NDM variants 1-17. The impact of NDM variants on structure is probed by comparing comparing melting temperature and refolding efficiency and also by spectroscopy (UV-Vis, 1 H-NMR, and EPR) of di-cobalt metalloforms...
June 16, 2018: Journal of Biological Chemistry
Jinghao Qin, Hongbing Shi, Yanjie Xu, Fang Zhao, Qing Wang
This study aims to explore the effects and related mechanisms of Tanshinone IIA in cervix carcinoma (CC) stemness-like cells migration, invasion, stemness and chemotherapeutical sensitivity. Here, we found that Tanshinone IIA suppressed CC stemness-like cells migration and invasion in a concentration- and time-dependent manner. And consistent results were obtained in CC cells stemness characterized as the decrease of CC stemness markers expression and cells spheroid formation ability. Mechanistically, we found that Tanshinone IIA suppressed RNA binding protein HuR translocation from nuclear to cytoplasm, and thus reduced YAP mRNAs stability and transcriptional activity...
June 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Fabienne Post, Silvia Pardeller, Beatrice Frajo-Apor, Georg Kemmler, Catherine Sondermann, Armand Hausmann, W Wolfgang Fleischhacker, Yuya Mizuno, Hiroyuki Uchida, Alex Hofer
OBJECTIVES: Improving Quality of Life (QoL) is an important objective in the treatment of bipolar disorder. The aim of the current study was to examine to which extent resilience, internalized stigma, and psychopathology are correlated to QoL. METHODS: We recruited 60 outpatients diagnosed with bipolar I disorder according to DSM-IV criteria and 77 healthy control subjects from the general community. In patients, symptoms were quantified by the Montgomery-Åsberg Depression Rating Scale (MADRS) and the Young Mania Rating Scale (YMRS) and internalized stigma by the Internalized Stigma of Mental Illness (ISMI) scale...
May 29, 2018: Journal of Affective Disorders
Claus Schwechheimer
The ubiquitin-related protein NEDD8 is conjugated and deconjugated to and from proteins in processes related to ubiquitin conjugation and deconjugation. Neddylation is a well-studied posttranslational modification of Cullin-RING E3 ligases (CRLs). Biochemical and structural studies aiming at understanding the role of NEDD8 in CRL function have now resulted in a convincing model of how neddylation and deneddylation antagonistically regulate CRL stability, conformation, activity as well as degradation substrate receptor exchange...
June 14, 2018: Current Opinion in Plant Biology
Nattawee Poomsuk, Tirayut Vilaivan, Khatcharin Siriwong
Peptide nucleic acid (PNA) is a powerful biomolecule with a wide variety of important applications. In this work, the molecular structures and binding affinity of PNA with a D-prolyl-2-aminocyclopentane carboxylic acid backbone (acpcPNA) that binds to both DNA and RNA were studied using molecular dynamics simulations. The simulated structures of acpcPNA-DNA and acpcPNA-RNA duplexes more closely resembled the typical structures of B-DNA and A-RNA than the corresponding duplexes of aegPNA. The calculated binding free energies are in good agreement with the experimental results that the acpcPNA-DNA duplex is more stable than the acpcPNA-RNA duplex regardless of the base sequences...
June 12, 2018: Journal of Molecular Graphics & Modelling
P Salvador-Coloma, M D Arguisuelas, J Doménech-Fernández, D Sánchez-Zuriaga, J J Amer-Cuenca, J Martínez-Gramage, F J Montañez-Aguilera, J F Lisón
INTRODUCTION: Unstable shoes were developed as a walking device to strengthen the lower extremity muscles and reduce joint loading. Many studies have reported increased muscle activity throughout the gait cycle in most of the lower limb muscles in healthy adults using these shoes. However, no previous studies have explored the effects of wearing unstable shoes on trunk muscle activity in patients with chronic low back pain (CLBP). Therefore, the aim of the present study was to compare the activity of selected trunk muscles in patients with CLBP during a gait test while walking wearing unstable shoes or conventional flat shoes (control)...
June 11, 2018: Gait & Posture
Yun-Chung Lin, Chi-Chien Niu, Mohammad Nikkhoo, Meng-Ling Lu, Wen-Chien Chen, Chen-Ju Fu, Chih-Hsiu Cheng
BACKGROUND: Degenerative lumbar diseases (DLDs) are characterized by motor functional deficits and postural instability. In this study, we investigated the differences in the trunk muscle responses to postural control between the presurgical DLD patients and healthy individuals while performing the static and perturbed balance tasks. METHODS: Thirty-five DLD patients (aged 61.1 ± 8.0 years) and thirty-five asymptomatic controls (aged 62.9 ± 3.7 years) participated in this study...
June 11, 2018: Gait & Posture
Buse Ozcan Kahraman, Turhan Kahraman, Orhan Kalemci, Yesim Salik Sengul
BACKGROUND: Many studies have reported that there are several differences between genders which may result in altered neuromuscular control. Although the existing evidence suggests that low back pain (LBP) affects the ability to control posture, there is little evidence the gender differences in postural control in people with nonspecific chronic LBP. RESEARCH QUESTION: Are there any gender differences in postural control and correlations between postural control, pain, disability, and fear of movement in people with nonspecific chronic LBP? METHODS: Static and dynamic postural control were evaluated using a computerized postural control assessment tool including assessments for limits of stability (LOS), unilateral stance, and modified clinical test of sensory interaction on balance...
June 11, 2018: Gait & Posture
Yang Liu, Xueqian Chen, Qiang Ma
In this work, a novel surface plasmon resonance (SPR) enhanced electrochemiluminescence (ECL) biosensing model was first designed based on Au NPs@polydopamine (PDA)@CuInZnS QDs nanocomposite. Au NPs were coated with the PDA layer via the electrostatic force. CuInZnS QDs were bound on the surface of Au NPs@PDA nanocomposite. CuInZnS QDs worked as ECL luminophore in the sensing application. PDA shell not only controlled the separation length between Au NPs and QDs to induce SPR enhanced ECL response, but also limited the potential charge transfer and ECL quenching effect...
June 9, 2018: Biosensors & Bioelectronics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"