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https://www.readbyqxmd.com/read/27911484/platinum-complex-assemblies-as-luminescent-probes-and-tags-for-drugs-and-toxins-in-water
#1
Stephan Sinn, Frank Biedermann, Luisa De Cola
A reactive phosphorescent probe for aza-heterocyclic drugs and toxins was developed, affording a supramolecular emission-switch-on chemosensor in water. Complex formation of the heterocycles with a platinum(II) precursor proceeds readily at ambient conditions, allowing for facile analyte screening. 52 structurally diverse compounds were tested, out of which 23 pyridines, imidazole, and triazoles formed strongly emissive complex aggregates. Importantly, they all can be clearly distinguished from each other through a principal component analysis, but often already by simple visual inspection, e...
December 1, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27911427/-modern-approach-to-early-diagnosis-and-pathogenetic-treatment-of-diabetic-retinopathy
#2
I V Vorob'eva
AIM: To improve the approach to pathogenetic treatment of diabetic retinopathy (DR) through early diagnosis and a new method for predicting disease progression. MATERIAL AND METHODS: The study enrolled 330 type 2 diabetes patients with DR (660 eyes), of whom women constituted 64.6%, men - 35.4%. The mean patient age was 62.3±2.3 years. Three groups were formed: the controls - 30 healthy volunteers (60 eyes) and 30 type 2 diabetes patients without ocular involvement (DR 0, 60 eyes); group 1 - 30 type 2 diabetes patients with DR I but no diabetic macular edema (DR I without DME, 60 eyes) that were treated with calcium dobesilate; group 2 - 240 type 2 diabetes patients, who had diabetic retinopathy of different stages (DR I, II, or III with DME, 480 eyes) and received laser retinal photocoagulation (LRP)...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27911403/preparation-and-reactivity-of-a-triphosphenium-bromide-salt-a-convenient-and-stable-source-of-phosphorus-i
#3
Stephanie C Kosnik, Justin F Binder, Maxemilian C Nascimento, Charles L B Macdonald
We present herein the optimized synthesis of a triphosphenium bromide salt. Apart from being a versatile metathesis reagent, this unusually stable low-valent-phosphorus-containing compound acts as a useful P(+) transfer agent. Unlike traditional methods employed to access low-coordinate phosphorus species which usually require pyrophoric phosphorus-containing precursors (white phosphorus, Tris(trimethylsilyl)phosphine, etc.), or harsh reducing agents (alkali metals, potassium graphite, etc.), the current approach does not involve pyrophoric or explosive reagents and can be done on large scales (>20 g) in excellent yields by undergraduates with basic air-free synthetic training...
November 22, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911384/analysis-of-scap-n-glycosylation-and-trafficking-in-human-cells
#4
Chunming Cheng, Jeffrey Yunhua Guo, Feng Geng, Xiaoning Wu, Xiang Cheng, Qiyue Li, Deliang Guo
Elevated lipogenesis is a common characteristic of cancer and metabolic diseases. Sterol regulatory element-binding proteins (SREBPs), a family of membrane-bound transcription factors controlling the expression of genes important for the synthesis of cholesterol, fatty acids and phospholipids, are frequently upregulated in these diseases. In the process of SREBP nuclear translocation, SREBP-cleavage activating protein (SCAP) plays a central role in the trafficking of SREBP from the endoplasmic reticulum (ER) to the Golgi and in subsequent proteolysis activation...
November 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911378/iridium-oxide-reduced-graphene-oxide-nanohybrid-thin-film-modified-screen-printed-electrodes-as-disposable-electrochemical-paper-microfluidic-ph-sensors
#5
Jiang Yang, Tae-Joon Kwak, Xiaodong Zhang, Robert McClain, Woo-Jin Chang, Sundaram Gunasekaran
A facile, controllable, inexpensive and green electrochemical synthesis of IrO2-graphene nanohybrid thin films is developed to fabricate an easy-to-use integrated paper microfluidic electrochemical pH sensor for resource-limited settings. Taking advantages from both pH meters and strips, the pH sensing platform is composed of hydrophobic barrier-patterned paper micropad (µPAD) using polydimethylsiloxane (PDMS), screen-printed electrode (SPE) modified with IrO2-graphene films and molded acrylonitrile butadiene styrene (ABS) plastic holder...
November 22, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911365/low-energy-cathodoluminescence-for-oxy-nitride-phosphors
#6
Yujin Cho, Benjamin Dierre, Takashi Sekiguchi, Takayuki Suehiro, Kohsei Takahashi, Takashi Takeda, Rong-Jun Xie, Yoshinobu Yamamoto, Naoto Hirosaki
Nitride and oxynitride (Sialon) phosphors are good candidates for the ultraviolet and visible emission applications. High performance, good stability and flexibility of their emission properties can be achieved by controlling their composition and dopants. However, a lot of work is still required to improve their properties and to reduce the production cost. A possible approach is to correlate the luminescence properties of the Sialon particles with their local structural and chemical environment in order to optimize their growth parameters and find novel phosphors...
November 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911364/an-intramedullary-locking-nail-for-standardized-fixation-of-femur-osteotomies-to-analyze-normal-and-defective-bone-healing-in-mice
#7
Tina Histing, Michael D Menger, Tim Pohlemann, Romano Matthys, Tobias Fritz, Patric Garcia, Moritz Klein
Bone healing models are essential to the development of new therapeutic strategies for clinical fracture treatment. Furthermore, mouse models are becoming more commonly used in trauma research. They offer a large number of mutant strains and antibodies for the analysis of the molecular mechanisms behind the highly differentiated process of bone healing. To control the biomechanical environment, standardized and well-characterized osteosynthesis techniques are mandatory in mice. Here, we report on the design and use of an intramedullary nail to stabilize open femur osteotomies in mice...
November 13, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911359/directed-protein-packaging-within-outer-membrane-vesicles-from-escherichia-coli-design-production-and-purification
#8
Nathan J Alves, Kendrick B Turner, Scott A Walper
An increasing interest in applying synthetic biology techniques to program outer membrane vesicles (OMV) are leading to some very interesting and unique applications for OMV where traditional nanoparticles are proving too difficult to synthesize. To date, all Gram-negative bacteria have been shown to produce OMV demonstrating packaging of a variety of cargo that includes small molecules, peptides, proteins and genetic material. Based on their diverse cargo, OMV are implicated in many biological processes ranging from cell-cell communication to gene transfer and delivery of virulence factors depending upon which bacteria are producing the OMV...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911265/mirna-543-promotes-osteosarcoma-cell-proliferation-and-glycolysis-by-partially-suppressing-prmt9-and-stabilizing-hif-1%C3%AE-protein
#9
Heng Zhang, Xiaofeng Guo, Xing Feng, Tingting Wang, Zhaohua Hu, Xiangyong Que, Qingsong Tian, Tianbo Zhu, Guixian Guo, Wei Huang, Xinzhi Li
Osteosarcoma (OS) is the most common primary bone tumor, occurring frequently in adolescents and possessing a high malignant severity. MicroRNAs play critical roles during OS development. Thus, elucidation of the involvement of specific microRNAs in the development of OS may provide novel therapeutic targets for OS treatment. Here, we showed that in the OS specimens from patients, the levels of miR-543 were significantly increased whereas the levels of PRMT9 were significantly decreased, compared to the paired normal bone tissue...
November 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27911256/a-residue-located-at-the-junction-of-the-head-and-stalk-regions-of-measles-virus-fusion-protein-regulates-membrane-fusion-by-controlling-conformational-stability
#10
Yuto Satoh, Saeka Yonemori, Mitsuhiro Hirose, Hiroko Shogaki, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Tsuyoshi Shirai, Ken-Ichi Takahashi, Masae Itoh
The fusion (F) protein of measles virus (MV) performs refolding from the thermodynamically metastable prefusion form to the highly stable postfusion form via an activated unstable intermediate stage, to induce membrane fusion. Some amino acids involved in the fusion regulation cluster in the heptad repeat B (HR-B) domain of the stalk region, among which substitution of residue 465 by various amino acids revealed that fusion activity correlates well with its side chain length from the Cα (p<0.01) and van der Waals volume (p<0...
December 1, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27911222/the-histone-methyltransferases-set5-and-set1-have-overlapping-functions-in-gene-silencing-and-telomere-maintenance
#11
Meagan Jezek, Alison Gast, Grace Choi, Rushmie Kulkarni, Jeremiah Quijote, Andrew Graham-Yooll, DoHwan Park, Erin M Green
Genes adjacent to telomeres are subject to transcriptional repression mediated by an integrated set of chromatin modifying and remodeling factors. The telomeres of Saccharomyces cerevisiae have served as a model for dissecting the function of diverse chromatin proteins in gene silencing, and their study has revealed overlapping roles for many chromatin proteins in either promoting or antagonizing gene repression. The H3K4 methyltransferase Set1, which is commonly linked to transcriptional activation, has been implicated in telomere silencing...
December 2, 2016: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/27911050/tuning-the-reactivity-of-graphene-by-surface-phase-orientation
#12
Jan Plsek, Petr Kovaricek, Vaclav Vales, Martin Kalbac
Tuning the local reactivity of graphene is a subject of paramount importance. Among the available strategies, the activation/passivation of graphene by copper substrate is very promising, because it enables to influence the properties of graphene without any transfer procedure as graphene can be grown directly on copper. Here, we demonstrate that the reactivity of graphene towards fluorination is strongly influenced by the face of the surface of the copper substrate. We probed graphene on the copper foil with identified grain orientations...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27911048/fluorescent-oligonucleotide-probes-for-screening-high-affinity-nucleobase-surrogates
#13
Asmo Aro-Heinilä, Tuomas Lönnberg
Double-helical oligonucleotide probes featuring a single-nucleotide gap opposed by one of the canonical nucleobases and flanked by the fluorescent nucleobase analogue pyrrolocytosine have been synthesized and titrated with PdII chelates of dipicolinamide and its N2,N6-dialkylated derivatives. The fluorometric titrations revealed greatly increased affinity of the PdII chelates for the nucleobases opposing the gap compared to the respective free nucleotides in solution. Owing to the constrained environment of the single-nucleotide gap, the relative stabilities of the various PdII mediated base pairs were also significantly different from those previously reported at monomer level...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27911043/a-cyclic-alkyl-amino-carbene-as-two-atom-%C3%AF-chromophore-leading-to-the-first-phosphorescent-linear-cu-i-complexes
#14
Markus Gernert, Ulrich Müller, Martin Haehnel, Jens Pflaum, Andreas Steffen
A series of linear and trigonal copper(I) complexes bearing a cyclic alkyl(amino)carbene (CAAC) ligand show surprising photophysical properties when compared to their corresponding N-heterocyclic carbene (NHC) complexes. Whereas the linear NHC complexes [CuX(NHC)] are nearly non-emissive, [CuX(CAAC)] (X = Cl, Br, I) and [Cu(CAAC)2]PF6 show very bright emission from their triplet excited states in the blue to green region displaying quantum yields of up to 65% in the solid state, although the π-acceptor comprises only the carbene C and N atoms with no other π conjugation...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27910984/enzyme-immobilized-clay-nanotube-chitosan-membranes-with-sustainable-biocatalytic-activities
#15
Jiajia Sun, Raghuvara Yendluri, Kai Liu, Ying Guo, Yuri Lvov, Xuehai Yan
We have developed a simple and effective strategy to prepare an enzymatic membrane by the admixing of a halloysite clay nanotube-lipase complex and a chitosan solution. The loading of 3 wt% lipase into the lumen of halloysite clay nanotubes was enhanced with an electrostatic interaction. 10 wt% of lipase-nanotubes were dispersed in an aqueous chitosan solution and cast as a coating. The enzymatic chitosan-halloysite membranes displayed increased pH, temperature stability and long-term usability for the catalytic decomposition of lipids: 90% of the initial activity was preserved at 80 °C, 70% of the functionality remained at the high pH of 9, and 85% of the activity was sustained after ten continuous cycles of use...
December 2, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27910981/cu-cdots-nanocorals-as-electrocatalyst-for-highly-efficient-co2-reduction-to-formate
#16
Sijie Guo, Siqi Zhao, Jin Gao, Cheng Zhu, Xiuqin Wu, Yijun Fu, Hui Huang, Yang Liu, Zhenhui Kang
Electrochemical reduction of CO2 is a key component of many prospective artificial technologies for renewable carbon-containing fuels, but it still suffers from the high overpotentials required to drive the process, low selectivity for diversiform products and the high cost of the catalyst. Here, we report that Cu-CDots nanocorals is a highly efficient, low-cost and stable electrocatalyst for CO2 reduction in aqueous solution. The major product of CO2 reduction on the Cu-CDots nanocorals is HCOOH with an inconceivable low overpotential of 0...
December 2, 2016: Nanoscale
https://www.readbyqxmd.com/read/27910975/heterogeneous-pegylation-of-diamond-nanoparticles
#17
Amanda S Barnard
Coating the surfaces of inorganic nanoparticles with polyethylene glycol (PEG) is an important step in the development of many nanoparticle-based drug delivery systems. The efficiency with which drug molecules can be loaded on to nanoparticle surfaces is contingent on the concentration, distribution and stability of the PEG coating. In this study the distribution and relative stability of PEG on diamond nanoparticles is predicted, for clean and passivated surface structures, in 3D. This is an ideal exemplar, since PEGylated diamond nanoparticles are already being trialed as carriers for doxorubicin (DOX)...
December 2, 2016: Nanoscale
https://www.readbyqxmd.com/read/27910969/the-intramolecular-h-bonding-effect-on-the-growth-and-stability-of-schiff-base-surface-covalent-organic-frameworks
#18
Yi-Ping Mo, Xuan-He Liu, Bing Sun, Hui-Juan Yan, Dong Wang, Li-Jun Wan
The introduction of intramolecular H-bonding by adding -OH functionalities adjacent to the Schiff base centers is considered to be a useful strategy to enhance the stability and crystallinity of bulk covalent organic frameworks (COFs). However, the influence of intramolecular H-bonding on the synthesis of surface COFs (SCOFs) have been barely explored. Herein, SCOFs based on the Schiff-base reaction between 1,3,5-tris(4-aminophenyl)benzene (TAPB) and terephthalaldehydes with symmetry or asymmetrically substituted hydroxyl functional groups are designed...
December 2, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27910948/improved-in-cell-structure-determination-of-proteins-at-near-physiological-concentration
#19
Teppei Ikeya, Tomomi Hanashima, Saori Hosoya, Manato Shimazaki, Shiro Ikeda, Masaki Mishima, Peter Güntert, Yutaka Ito
Investigating three-dimensional (3D) structures of proteins in living cells by in-cell nuclear magnetic resonance (NMR) spectroscopy opens an avenue towards understanding the structural basis of their functions and physical properties under physiological conditions inside cells. In-cell NMR provides data at atomic resolution non-invasively, and has been used to detect protein-protein interactions, thermodynamics of protein stability, the behavior of intrinsically disordered proteins, etc. in cells. However, so far only a single de novo 3D protein structure could be determined based on data derived only from in-cell NMR...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910930/design-of-a-hydroxyapatite-binding-antimicrobial-peptide-with-improved-retention-and-antibacterial-efficacy-for-oral-pathogen-control
#20
Zhi-Bin Huang, Xin Shi, Jing Mao, Shi-Qiang Gong
Controlling and reducing the formation of pathogenic biofilm on tooth surface is the key to the prevention and treatment of the biofilm-associated oral diseases. Antimicrobial peptides (AMPs), considered as possible future alternatives for conventional antibiotics, have been extensively studied for the control of bacterial infection. Due to the rapid dilution and degradation by human saliva, AMP preparations designed for oral use with longer retention and higher efficacy are in urgent need. To this end, a hydroxyapatite (HAp)-binding antimicrobial peptide (HBAMP), which is based on the fusion of a specific HAp-binding heptapeptide (HBP7) domain and a broad-spectrum antimicrobial peptide (KSLW) domain, has been developed in our laboratory...
December 2, 2016: Scientific Reports
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