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https://www.readbyqxmd.com/read/28743116/the-role-of-tumoral-foxp3-on-cell-proliferation-migration-and-invasion-in-gastric-cancer
#1
Lu Zhang, Jianghao Xu, Xuan Zhang, Yi Zhang, Lu Wang, Xiaoxu Huang, Zekuan Xu
BACKGROUND/AIMS: There is little published data on the role of FOXP3 in gastric cancer. METHODS: FOXP3 expression and localization in gastric cancer tissues and cells were examined by immunohistochemistry, RT-PCR, flow cytometry, western blot, and laser confocal microscopy. CCK8, plate clone, wound healing, and transwell insert assays were performed for gastric cancer cells. Potential molecules and signaling pathways were screened using high-throughput transcriptome sequencing...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28742200/hypoxia-promotes-epithelial-mesenchymal-transition-of-hepatocellular-carcinoma-cells-via-inducing-twist1-expression
#2
X-D Zhang, X-Q Dong, J-L Xu, S-C Chen, Z Sun
OBJECTIVE: To investigate whether hypoxia microenvironment induced hepatocellular carcinoma cells SMMC-7721 epithelial-mesenchymal transition (EMT) and to explore the underlying molecular mechanism. MATERIALS AND METHODS: In this study, SMMC-7721 cells were cultured under normoxia and hypoxia conditions, respectively. RT-PCR and Western blot were used to monitor the expression level of EMT-related markers, E-cadherin, and vimentin, as well as hypoxia inducible factor-1α (HIF-1α) and Twist1...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28742199/cyramza-induces-apoptosis-of-hcc4006-cell-by-affecting-the-level-of-bcl-w
#3
L Fang, Z Li, Y-J Chen, G-M Xiao
OBJECTIVE: Lung cancer seriously threats to patient's life and health. Cyramza is a therapeutic drug for inhibition of vessel formation and growth in clinical practice. The aim of this study was to investigate the effect of cyramza on growth and apoptosis of non-small lung cancer HCC4006 and explore the related mechanisms. MATERIALS AND METHODS: Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Western blot was employed to examine the effect of cyramza on the apoptotic protein Bcl-w...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28742096/insecticide-resistance-is-mediated-by-multiple-mechanisms-in-recently-introduced-aedes-aegypti-from-madeira-island-portugal
#4
Gonçalo Seixas, Linda Grigoraki, David Weetman, José Luís Vicente, Ana Clara Silva, João Pinto, John Vontas, Carla Alexandra Sousa
BACKGROUND: Aedes aegypti is a major mosquito vector of arboviruses, including dengue, chikungunya and Zika. In 2005, Ae. aegypti was identified for the first time in Madeira Island. Despite an initial insecticide-based vector control program, the species expanded throughout the Southern coast of the island, suggesting the presence of insecticide resistance. Here, we characterized the insecticide resistance status and the underlying mechanisms of two populations of Ae. aegypti from Madeira Island, Funchal and Paúl do Mar...
July 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28741719/involvement-of-arabidopsis-glutaredoxin-s14-in-the-maintenance-of-chlorophyll-content
#5
Pascal Rey, Noëlle Becuwe, Sébastien Tourrette, Nicolas Rouhier
Plant class-II glutaredoxins (GRXs) are oxidoreductases carrying a CGFS active site signature and are able to bind iron-sulfur clusters in vitro. In order to explore the physiological functions of the two plastidial class-II isoforms, GRXS14 and GRXS16, we generated knockdown and overexpression Arabidopsis thaliana lines and characterized their phenotypes using physiological and biochemical approaches. Plants deficient in one GRX did not display any growth defect, whereas the growth of plants lacking both was slowed...
July 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28740552/npm1-mutant-mediated-pml-delocalization-and-stabilization-enhances-autophagy-and-cell-survival-in-leukemic-cells
#6
Qin Zou, Shi Tan, Zailin Yang, Qian Zhan, Hongjun Jin, Jingrong Xian, Shuaishuai Zhang, Liyuan Yang, Lu Wang, Ling Zhang
Accumulating evidence has defined nucleophosmin 1 (NPM1) mutation as a driver genetic event in acute myeloid leukemia (AML), whereas the pathogenesis of NPM1-mutated AML remains to be fully elucidated. In this study, we showed that mutant NPM1 elevated autophagic activity and autophagic activation contributed to leukemic cell survival in vitro. Meanwhile, we also found high expression of promyelocytic leukemia gene (PML) and its cytoplasmic dislocation in primary NPM1-mutated AML blasts and NPM1-mA positive OCI-AML3 cells...
2017: Theranostics
https://www.readbyqxmd.com/read/28739801/sustained-o-glcnacylation-reprograms-mitochondrial-function-to-regulate-energy-metabolism
#7
Ee Phie Tan, Steven R McGreal, Stefan Graw, Robert Tessman, Scott J Koppel, Pramod Dhakal, Zhen Zhang, Miranda Machacek, Natasha E Zachara, Devin C Koestler, Kenneth R Peterson, John P Thyfault, Russell H Swerdlow, Partha Krishnamurthy, Luciano DiTacchio, Udayan Apte, Chad Slawson
Dysfunctional mitochondria and generation of reactive oxygen species (ROS) promote chronic diseases, which have spurred interest in the molecular mechanisms underlying these conditions. Previously, we have demonstrated that disruption of post-translational modification of proteins with β-linked N-acetylglucosamine (O-GlcNAcylation) via overexpression of the O-GlcNAc regulating enzymes O-GlcNAc transferase (OGT) or O-GlcNAcase (OGA) impairs mitochondrial function. Here, we report that sustained alterations in O-GlcNAcylation either by pharmacological or genetic manipulation also alters metabolic function...
July 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28739744/overexpression-of-ephb4-is-associated-with-poor-survival-of-patients-with-gastric-cancer
#8
Jie Yin, Yuxin Cui, Liting Li, Jiafu Ji, Wen G Jiang
BACKGROUND: Increased expression of erythropoietin-producing human hepatoma (EPHB4) leads to enhanced cell migration, growth and adhesion in tumor cells. However, little is known regarding the effects of EPHB4 in gastric cancer. The present study aimed to examine the clinical relevance of EPHB4 and its association with the prognosis of gastric cancer. MATERIALS AND METHODS: EPHB4 transcript expression in 324 gastric cancer samples with paired adjacent normal gastric tissues was determined using quantitative polymerase chain reaction and the results were statistically analyzed against patient clinicopathological data...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739743/role-of-plexin-b1-in-a-breast-cancer-cohort-of-pakistani-patients-and-its-contribution-towards-cancer-metastasis-as-indicated-by-an-in-vitro-model
#9
Muhammad Faraz Arshad Malik, Syeda Kiran Riaz, S H Waqar, Farhan Haq, Lin Ye, Wen G Jiang
BACKGROUND/AIM: In the current study, the role of plexin B1 in breast cancer metastasis was explored. MATERIALS AND METHODS: Freshly-excised tumours along with background tissues of affected patients (n=121) were collected from Pakistani hospitals and processed for RNA isolation and cDNA synthesis. Using quantitative polymerase chain reaction, expression of plexin B1 was evaluated and correlated with clinicopathological parameters. Furthermore, involvement of plexin B1 in metastasis was explored by generating gene knockdown in MDA-MB-231 and MCF-7 breast cancer cells...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739735/knockdown-of-epha1-using-crispr-cas9-suppresses-aggressive-properties-of-ovarian-cancer-cells
#10
Yuxin Cui, B O Wu, Valentina Flamini, Bronwen A J Evans, Deshan Zhou, Wen G Jiang
BACKGROUND/AIM: Overexpression of erythropoietin-producing hepatocellular A1 (EPHA1), a member of the EPH super family, is frequently observed in various cancer types. The dysregulated interaction of EPHA1 with its ligand Ephrin A1 has been linked to the progression of ovarian cancer (OC). However, the contribution of EPHA1 in the regulation of the aggressive properties of OC cells remains unknown. MATERIALS AND METHODS: In this study we investigated the differential expression of EPHA1 in human OC cells...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739732/reduced-expression-of-ranbpm-is-associated-with-poorer-survival-from-lung-cancer-and-increased-proliferation-and-invasion-of-lung-cancer-cells-in-vitro
#11
Zehang Zhao, Shan Cheng, Catherine Zabkiewicz, Jinfeng Chen, Lijiang Zhang, Lin Ye, Wen G Jiang
BACKGROUND/AIM: Ran binding protein microtubule-organizing centre (RanBPM), also known as RanBP9, is a scaffold protein conserved through evolution. We investigated the role of RanBPM in human lung cancer. MATERIALS AND METHODS: Transcripts of RanBPM were determined in 56 human lung cancers along with paired normal lung tissues using real-time PCR. Association with prognosis was analyzed by online Kaplan-Meier survival analysis. In vitro lung cancer cell functional assays examined the impact of RanBPM-knockdown on cellular growth and invasion...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739728/ezrin-radixin-moesin-binding-phosphoprotein-50-ebp50-suppresses-the-metastasis-of-breast-cancer-and-hela-cells-by-inhibiting-matrix-metalloproteinase-2-activity
#12
Lei Wang, Yijun Qi, Ying Xiong, Zhiqiang Peng, Qiang Ma, Yue Zhang, Jian Song, Junfang Zheng
BACKGROUND: Expression of ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) is correlated with human breast and cervical cancer development, but its effects on the metastasis of breast and cervical cancer and the underlying mechanism are not fully understood. MATERIALS AND METHODS: In this study, EBP50 was overexpressed in MDA-MB-231 breast cancer and HeLa cervical cancer cells; moreover, EBP50 was knocked-down in MCF-7 breast cancer cells and HeLa cells. Metastasis-related ability and matrix metalloproteinase-2 (MMP-2) activity of these cells were investigated...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739727/mir-141-inhibits-proliferation-and-migration-of-colorectal-cancer-sw480-cells
#13
Zhi H Long, Zhi G Bai, Jian N Song, Zhi Zheng, Jun Li, Jun Zhang, Jun Cai, Hong W Yao, Jin Wang, Ying C Yang, Jie Yin, Zhong T Zhang
BACKGROUND: This study was designed to determine the molecular function of miR-141 and the underlying mechanisms in colorectal cancer (CRC). MATERIALS AND METHODS: SW480 cells in which miR-141 was up- or down-regulated were established. Reverse transcription, quantitative polymerase chain reaction and Western blotting were used to examine the microRNA and protein expression. Cell-cycle progression was analyzed by flow cytometry. Proliferation marker Ki-67 was evaluated by immunofluorescence...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739717/potential-implication-of-paxillin-in-cancer-establishment-within-the-bone-environment
#14
Anna D Sobkowicz, Andrew J Sanders, Malcolm D Mason, Wen G Jiang
BACKGROUND: Bone metastases are a common feature of advanced prostatic malignancies. They are characterised by a unique prevalence of osteoblastic phenotype and a poor prognosis. Paxillin is a 68-kDa signal transduction adaptor and scaffold protein that contains motifs involved in the mediation of protein-protein interactions. The state of paxillin phosphorylation is central to determining a cell's ability to adhere, detach and migrate and hence has been linked to processes such as wound repair and tumour metastasis...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739643/histone-methyltransferase-dot1l-is-a-coactivator-for-thyroid-hormone-receptor-during-xenopus-development
#15
Luan Wen, Liezhen Fu, Yun-Bo Shi
Histone modifications are associated with transcriptional regulation by diverse transcription factors. Genome-wide correlation studies have revealed that histone activation marks and repression marks are associated with activated and repressed gene expression, respectively. Among the histone activation marks is histone H3 K79 methylation, which is carried out by only a single methyltransferase, disruptor of telomeric silencing-1-like (DOT1L). We have been studying thyroid hormone (T3)-dependent amphibian metamorphosis in two highly related species, the pseudo-tetraploid Xenopus laevis and diploid Xenopus tropicalis, as a model for postembryonic development, a period around birth in mammals that is difficult to study...
July 24, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28739633/claudin-8d-is-a-cortisol-responsive-barrier-protein-in-the-gill-epithelium-of-trout
#16
Dennis Kolosov, Scott P Kelly
The influence of claudin (Cldn) 8 tight junction (TJ) proteins on cortisol-mediated alterations in gill epithelium permeability were examined using a primary cultured trout gill epithelium model. Genes encoding three Cldn-8 proteins (cldn-8b, -8c and -8d) have been identified in trout and all are expressed in the model gill epithelium. Cortisol treatment 'tightened' the gill epithelium, as indicated by increased transepithelial resistance (TER) and reduced paracellular [3H]polyethylene glycol (MW 400 kDa; PEG-400) flux...
July 24, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28739548/the-role-of-mir-210-e2f3-and-ephrin-a3-in-angiosarcoma-cell-proliferation
#17
Satoko Nakashima, Masatoshi Jinnin, Hisashi Kanemaru, Ikko Kajihara, Toshikatsu Igata, Sayo Okamoto, Yukino Tazaki, Miho Harada, Shinichi Masuguchi, Satoshi Fukushima, Mamiko Masuzawa, Yasuyuki Amoh, Mikio Masuzawa, Hironobu Ihn
BACKGROUND: Although angiosarcoma exhibits aggressive progression and is associated with unfavourable prognosis, its pathogenesis is poorly understood. OBJECTIVES: In the present study, we investigated the possibility that microRNAs play a role in the pathogenesis of angiosarcoma. MATERIALS & METHODS: microRNA expression was evaluated by array analysis and real-time PCR, and protein expression was determined by immunohistochemistry and immunoblotting...
July 25, 2017: European Journal of Dermatology: EJD
https://www.readbyqxmd.com/read/28739494/amblyomma-maculatum-secis-binding-protein-2-and-putative-selenoprotein-p-are-indispensable-for-pathogen-replication-and-tick-fecundity
#18
Khemraj Budachetri, Gary Crispell, Shahid Karim
Selenium, a vital trace element, is incorporated into selenoproteins to produce selenocysteine. Our previous studies have revealed an adaptive co-evolutionary process that has enabled the spotted fever-causing tick-borne pathogen Rickettsia parkeri to survive by manipulating an antioxidant defense system associated with selenium, which includes a full set of selenoproteins and other antioxidants in ticks. Here, we conducted a systemic investigation of SECIS binding protein 2 (SBP2) and putative selenoprotein P (SELENOP) by transcript silencing in adult female Gulf-coast ticks (Amblyomma maculatum)...
July 21, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28739472/toll-like-receptor-4-signaling-improved-the-migration-of-sca-1-stem-progenitor-cells
#19
Ying Huang, Chunya Zhang, Jinghua Shen, Xiaogang Zhang, Jianqing Du, Daifu Zhang
Atherosclerosis is considered as a chronic inflammatory process, during which macrophages and smooth muscle cells migrate into the vascular wall intima to initiate atherogenesis. Stem/progenitor cells (SPCs) have been demonstrated as a new source of monocytes/macrophages and smooth muscle cells (SMCs). However, the regulation of SPCs migration into atherosclerotic initiation sites to produce macrophages and SMCs in-situis not fully elucidated. As toll-like receptor (TLRs) signaling plays essential role in migration of immunocytes, we wanted to explore the expression and function of TLRs in SPCs in detail...
July 21, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28739412/alginate-enhances-toll-like-receptor-4-mediated-phagocytosis-by-murine-raw264-7-macrophages
#20
Decheng Bi, Rui Zhou, Nan Cai, Qiuxian Lai, Qingguo Han, Yanwen Peng, Zedong Jiang, Zhishu Tang, Jun Lu, Weiyang Bao, Hong Xu, Xu Xu
Alginate is a naturally acidic polysaccharide consisting alternately of β-D-mannuronic acid and α-L-guluronic acid with 1, 4-glycosidic linkages and is derived from brown seaweeds. Herein, the effect of alginate on the promotion of macrophage phagocytosis and the corresponding molecular mechanisms were investigated in murine RAW264.7 cells. Alginate could enhance the intracellular phagocytosis of gold nanoparticles (AuNPs), fluorescent microspheres and immunoglobulin G (IgG)-opsonized Staphylococcus aureus (S...
July 21, 2017: International Journal of Biological Macromolecules
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