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Hongbo Xie, Jie Wu, Dan Liu, Mingyi Liu, Hong Zhang, Shibo Huang, Yuqing Xiong, Chunhua Xia
1. Ursolic acid (UA) and oleanolic acid (OA) may have important activity relevant to health and disease prevention. Thus, we studied the activity of UA and OA on UDP-glucuronosyltransferases (UGTs) and used trifluoperazine as a probe substrate to test UGT1A4 activity. Recombinant UGT-catalyzed 4-methylumbelliferone (4-MU) glucuronidation was used as a probe reaction for other UGT isoforms. 2. UA and OA inhibited UGT1A3 and UGT1A4 activity but did not inhibit other tested UGT isoforms. 3. UA-mediated inhibition of UGT1A3 catalyzed 4-MU-β-D-glucuronidation was via competitive inhibition (IC50 0...
September 7, 2016: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Valerie M Harris, Rohan Sharma, Joshua Cavett, Biji T Kurien, Ke Liu, Kristi A Koelsch, Astrid Rasmussen, Lida Radfar, David Lewis, Donald U Stone, C Erick Kaufman, Shibo Li, Barbara Segal, Daniel J Wallace, Michael H Weisman, Swamy Venuturupalli, Jennifer A Kelly, Marta E Alarcon-Riquelme, Bernardo Pons-Estel, Roland Jonsson, Xianglan Lu, Jacques-Eric Gottenberg, Juan-Manuel Anaya, Deborah S Cunninghame-Graham, Andrew J W Huang, Michael T Brennan, Pamela Hughes, Ilias Alevizos, Corinne Miceli-Richard, Edward C Keystone, Vivian P Bykerk, Gideon Hirschfield, Gang Xie, Wan-Fai Ng, Gunnel Nordmark, Sara Magnusson Bucher, Per Eriksson, Roald Omdal, Nelson L Rhodus, Maureen Rischmueller, Michael Rohrer, Marie Wahren-Herlenius, Torsten Witte, Xavier Mariette, Christopher J Lessard, John B Harley, Kathy L Sivils, R Hal Scofield
Primary Sjögren's syndrome (pSS) has a strong female bias. We evaluated an X chromosome dose effect by analyzing 47,XXY (Klinefelter's syndrome, 1 in 500 live male births) among subjects with pSS. 47,XXY was determined by examination of fluorescence intensity of single nucleotide polymorphisms from the X and Y chromosomes. Among 136 pSS men there were 4 with 47,XXY. This was significantly different from healthy controls (1 of 1254 had 47,XXY, p=0.0012 by Fisher's exact test) as well men with rheumatoid arthritis (0 of 363 with 47,XXY), but not different compared to men with systemic lupus erythematosus (SLE) (4 of 136 versus 8 of 306, Fisher's exact test p=NS)...
July 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Xinrong Tao, Tania Garron, Anurodh Shankar Agrawal, Abdullah Algaissi, Bi-Hung Peng, Maki Wakamiya, Teh-Sheng Chan, Lu Lu, Lanying Du, Shibo Jiang, Robert B Couch, Chien-Te K Tseng
UNLABELLED: Characterized animal models are needed for studying the pathogenesis of and evaluating medical countermeasures for persisting Middle East respiratory syndrome-coronavirus (MERS-CoV) infections. Here, we further characterized a lethal transgenic mouse model of MERS-CoV infection and disease that globally expresses human CD26 (hCD26)/DPP4. The 50% infectious dose (ID50) and lethal dose (LD50) of virus were estimated to be <1 and 10 TCID50 of MERS-CoV, respectively. Neutralizing antibody developed in the surviving mice from the ID50/LD50 determinations, and all were fully immune to challenge with 100 LD50 of MERS-CoV...
October 7, 2015: Journal of Virology
Hao Wu, Shibo Xia, Fanyun Bu, Jing Qi, Yuqing Liu, Hai Xu
The dissemination of drug-resistant Escherichia coli in poultry products is becoming a public concern, as it endangers food security and human health. It is very common for E. coli to exhibit drug resistance in the poultry industry in China due to the excessive use of antibiotics. However, few studies have examined the drug resistance endowed by integrons and integron-associated gene cassettes in different phylogenetic groups of E. coli isolated from broiler carcasses. In this study, 373 antibiotic-resistant E...
October 15, 2015: International Journal of Food Microbiology
Rudragouda Channappanavar, Lu Lu, Shuai Xia, Lanying Du, David K Meyerholz, Stanley Perlman, Shibo Jiang
To gain entry into the target cell, Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) uses its spike (S) protein S2 subunit to fuse with the plasma or endosomal membrane. Previous work identified a peptide derived from the heptad repeat (HR) 2 domain in S2 subunit, HR2P, which potently blocked MERS-CoV S protein-mediated membrane fusion. Here, we tested an HR2P analogue with improved pharmaceutical property, HR2P-M2, for its inhibitory activity against MERS-CoV infection in vitro and in vivo. HR2P-M2 was highly effective in inhibiting MERS-CoV S protein-mediated cell-cell fusion and infection by pseudoviruses expressing MERS-CoV S protein with or without mutation in the HR1 region...
December 15, 2015: Journal of Infectious Diseases
Shibo Xi, Lucas Santiago Borgna, Yonghua Du
It is essential but inconvenient to perform high-quality on-line optimization for synchrotron radiation beamlines. Usually, synchrotron radiation beamlines are optimized manually, which is time-consuming and difficult to obtain global optimization for all optical elements of the beamline. In this contribution a general method based on the genetic algorithm for automatic beamline optimization is introduced. This method can optimize all optical components of any beamline simultaneously and efficiently. To test this method, a program developed using LabVIEW is examined at the XAFCA beamline of the Singapore Synchrotron Light Source to optimize the beam flux at the sample position...
May 2015: Journal of Synchrotron Radiation
Tiemin Zhang, Wei Zhao, Dong Yang, Daxun Piao, Shibo Huang, Yuanyuan Mi, Xianqi Zhao, Jianping Cao, Yujuan Shen, Weizhe Zhang, Aiqin Liu
BACKGROUND: Cystic echinococcosis (CE) is one of emerging zoonotic parasitic diseases throughout the world, having significant medical and economic importance in developing countries. The western and northwestern China is considered as CE endemic areas. In northeastern China's Heilongjiang Province, the increasing number of sporadic human CE cases has attracted more and more attention. The aims of the present study were to understand the clinical characteristics of human CE in the investigated area and to compare the coincidence rates of CT, ultrasound and serological test against the histopathology results among CE patients...
2015: BMC Gastroenterology
Congji Wen, Hai Yan, Shibo Fu, Yunliang Qian, Danru Wang, Chen Wang
Adipose-derived stem cells (ASCs) with multilineage potential can be induced into osteoblasts, adipocytes and chondrocytes. ASCs as seed cell are widely used in the field of tissue engineering, but most studies either use autologous cells as the source or an immunodeficient animal as the host. In our present study, we explored the feasibility of applying allogeneic ASCs and demineralized bone matrix (DBM) scaffolds for repairing tubular bone defects without using immunosuppressive therapy. Allogeneic ASCs were expanded and seeded on DBM scaffolds and induced to differentiate along the osteogenic lineage...
July 2016: Experimental Biology and Medicine
Gang Chen, Shibo Cui, Lin You, Yan Li, Yun-Hui Mei, Xu Chen
Tension, single-step creep, and multi-step creep of rat skins at room temperature were experimentally studied. We studied the effects of loading histories of high stress creep, low stress creep, and stress relaxation on multi-step creep. Microstructure of rat skins after prescribed tests were observed microscopically with the help of standard hematoxylin and eosin (H&E). The void ratios were also analyzed. The loading histories of high stress creep, low stress creep, and stress relaxation have significant influence on multi-step creep...
June 2015: Journal of the Mechanical Behavior of Biomedical Materials
Mohamad Khawandanah, Bradley Gehrs, Shibo Li, Jennifer Holter Chakrabarty, Mohamad Cherry
Background. We hereby describe what we believe to be the first reported case of t (14; 15) (q32; q13) associated with acute myeloid leukemia (AML). Methods. PubMed, Embase, and OVID search engines were used to review the related literature and similar published cases. Case. A47-year-old female presented in December 2011 with AML (acute myelomonocytic leukemia) with normal cytogenetics; molecular testing revealed FLT-3 internal tandem duplication (ITD) mutation, while no mutations involving FLT3 D385/I836, NPM1 exon 12, or KIT exons 8 and 17 were detected...
2014: Case Reports in Genetics
Onofrio Zirafi, Kyeong-Ae Kim, Nadia R Roan, Silvia F Kluge, Janis A Müller, Shibo Jiang, Benjamin Mayer, Warner C Greene, Frank Kirchhoff, Jan Münch
Topically applied microbicides potently inhibit HIV in vitro but have largely failed to exert protective effects in clinical trials. One possible reason for this discrepancy is that the preclinical testing of microbicides does not faithfully reflect the conditions of HIV sexual transmission. We report that candidate microbicides that target HIV components show greatly reduced antiviral efficacy in the presence of semen, the main vector for HIV transmission. This diminished antiviral activity was dependent on the ability of amyloid fibrils in semen to enhance the infectivity of HIV...
November 12, 2014: Science Translational Medicine
Cuiqing Ma, Lili Wang, Xinrong Tao, Naru Zhang, Yang Yang, Chien-Te K Tseng, Fang Li, Yusen Zhou, Shibo Jiang, Lanying Du
The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) is currently spreading among humans, making development of effective MERS vaccines a high priority. A defined receptor-binding domain (RBD) in MERS-CoV spike protein can potentially serve as a subunit vaccine candidate against MERS-CoV infections. To identify an ideal vaccine candidate, we have constructed five different versions of RBD fragments, S350-588-Fc, S358-588-Fc, S367-588-Fc, S367-606-Fc, and S377-588-Fc (their names indicate their residue range in the spike protein and their C-terminal Fc tag), and further investigated their receptor binding affinity, antigenicity, immunogenicity, and neutralizing potential...
October 21, 2014: Vaccine
Cynthia M Magro, Ronnie M Abraham, Ruifeng Guo, Shibo Li, Xuan Wang, Steven Proper, A Neil Crowson, Martin Mihm
BACKGROUND: Deep penetrating nevi (DPN) are a relatively uncommon subtype of melanocytic nevi. A small subset of these lesions exhibit atypical features (cytologic and architectural atypia, mitotic activity) seen in melanoma. These lesions we term the deep penetrating nevus-like borderline tumor. Unequivocal melanomas can show overlapping morphologic features of DPN, which have been termed plexiform melanomas. PATIENTS AND METHODS: 40 cases of DPN-like borderline tumor were identified along with 6 cases of plexiform melanoma...
September 2014: European Journal of Dermatology: EJD
Na Liu, Bingjie Qin, Lian-Qi Sun, Fei Yu, Lu Lu, Shibo Jiang, Kuo-Hsiung Lee, Lan Xie
Using physicochemical property-driven optimization, twelve new diarylaniline compounds (DAANs) (7a-h, 11a-b and 12a-b) were designed and synthesized. Among them, compounds 12a-b not only showed high potency (EC50 0.96-4.92 nM) against both wild-type and drug-resistant viral strains with the lowest fold change (FC 0.91 and 5.13), but also displayed acceptable drug-like properties based on aqueous solubility and lipophilicity (LE>0.3, LLE>5, LELP<10). The correlations between potency and physicochemical properties of these DAAN analogues are also described...
August 15, 2014: Bioorganic & Medicinal Chemistry Letters
Minmin Li, Ruitao Zhang, Yiping Hu, Jianjun Li, Shibo Jiang, Xiaojuan Li, Shuwen Liu
OBJECTIVE: To observe if VIR576, an 20-mer peptide derived from the C-proximal subfragment of a1-antitrypsin (a1-AT) which inhibits human immunodeficiency virus type 1 (HIV-1) entry into the target cells by interacting with fusion peptide (FP), can also directly inhibit CD4(+) T cell activation in vitro. METHODS: Splenocytes isolated from DO11.10 OVA Tg mice were stimulated with ovalbumin or concanavalin A to test the effects of VIR576 on antigen-specific or non-antigen-specific T cell activation...
May 2014: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Tianlei Ying, Lanying Du, Tina W Ju, Ponraj Prabakaran, Candy C Y Lau, Lu Lu, Qi Liu, Lili Wang, Yang Feng, Yanping Wang, Bo-Jian Zheng, Kwok-Yung Yuen, Shibo Jiang, Dimiter S Dimitrov
The recently discovered Middle East respiratory syndrome coronavirus (MERS-CoV) continues to infect humans, with high mortality. Specific, highly effective therapeutics and vaccines against the MERS-CoV are urgently needed to save human lives and address the pandemic concerns. We identified three human monoclonal antibodies (MAbs), m336, m337, and m338, targeting the receptor (CD26/DPP4) binding domain (RBD) of the MERS-CoV spike glycoprotein from a very large naïve-antibody library (containing ∼10(11) antibodies)...
July 2014: Journal of Virology
Weihong Xu, Xiao Yang, Xiaoxia Hu, Shibo Li
Neurofibromatosis type 1 (NF1) is a common autosomal dominant genetic disorder caused by mutations in the NF1 gene. One of the hallmarks of NF1 is the high mutation rate in this gene. In this study, we present 127 different NF1 mutations and 54 novel mutations detected at both the genomic DNA and mRNA level using a retrospective case series review. We found that 25.2% of these different mutations induced aberrant splicing. Of note, 40.6% of these splicing errors were caused by exonic variants. In addition, one mutation produced mosaicism in the post-transcriptional profile...
July 2014: International Journal of Molecular Medicine
Yang Huang, Zhenpeng Li, Hui Xing, Yang Jiao, Yabo Ouyang, Lingjie Liao, Shibo Jiang, Rebecca Armstrong, Yiming Shao, Liying Ma
BACKGROUND: The polymorphisms involved in drug resistance to non-nucleoside reverse transcriptase inhibitors (NNRTIs) in HIV-1 CRF_BC, the most prevalent HIV-1 strain in China, have been poorly characterized. RESULTS: To reveal the drug resistance mutations, we compared the gene sequences of pol region of HIV-1 CRF_BC from 631 treatment-naïve and 363 treatment-experienced patients using the selection pressure-based method. We calculated an individual Ka/Ks value for each specific amino acid mutation...
2014: PloS One
Cuiqing Ma, Zheng Liu, Wenjian Li, Xuesong Qian, Song Zhang, Xue Gao, Shibo Jiang, Lin Wei
We report the construction of a recombinant multivalent vaccine against group A streptococcus (GAS), designated F7M5. It contains seven predominant epitopes of FbaA identified by phage display technology, five non-tissue cross-reactive M protein fragments expressed on four selected serotypes prevalent in China, a Trojan antigen (TA) and a poly-alanine DR epitope (PADRE). BALB/c mice were immunized subcutaneously with F7M5 formulated with Freund's adjuvant, using recombinant FbaA and M protein in parallel as control...
May 2014: Microbes and Infection
Chao Wang, Weiguo Shi, Lifeng Cai, Lu Lu, Fei Yu, Qian Wang, Xifeng Jiang, Xiaoyu Xu, Kun Wang, Liang Xu, Shibo Jiang, Keliang Liu
OBJECTIVES: To develop new HIV-1 fusion inhibitors with improved antiviral activities and resistance profiles, we designed two categories of artificial peptides, each containing four heptad repeats (m4HR) conjugated with a pocket-specific small molecule (pssm) or pssm and cholesterol (chol), designated pssm-m4HR or pssm-m4HR-chol, respectively, and tested their anti-HIV-1 activity. METHODS: We synthesized the artificial peptides and conjugated these peptides with pssm and chol using a standard solid-phase Fmoc protocol and a chemoselective thioether conjugation method, respectively...
June 2014: Journal of Antimicrobial Chemotherapy
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