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Teresita M Porter, Mehrdad Hajibabaei
We introduce a method for assigning names to CO1 metabarcode sequences with confidence scores in a rapid, high-throughput manner. We compiled nearly 1 million CO1 barcode sequences appropriate for classifying arthropods and chordates. Compared to our previous Insecta classifier, the current classifier has more than three times the taxonomic coverage, including outgroups, and is based on almost five times as many reference sequences. Unlike other popular rDNA metabarcoding markers, we show that classification performance is similar across the length of the CO1 barcoding region...
March 9, 2018: Scientific Reports
Frida A Zink, Luke R Tembrock, Alicia E Timm, Todd M Gilligan
The silver Y moth [Autographa gamma (Linneaus) (Noctuidae: Plusiinae)] is a pervasive crop pest in its native range but has not been found in moth surveys in the United States. Specimens of A. gamma are often intercepted at U.S. ports of entry, so the risk of introduction of this invasive species is high. Currently, identification of Plusiinae adults captured in domestic surveys is done by morphlogical comparison; however, this method is time consuming and misidentifications have occurred in the past. A recent study outlined a real-time PCR assay capable of rapidly identifying individual A...
March 1, 2018: Journal of Economic Entomology
Minghua Zeng, Hui-Li Zheng, Xue-Li Chen, Tian Li, Zheng Yin, Yuexing Zhang, Mohamedally Kurmoo
Two heptanuclear disks of [Co7(L1)6(CH3O)6]2+ (Co7-L1) and [Co7(L2)12](ClO4)2 (Co7-L2) were obtained using Schiff base (HL1 = 2-methoxy-6-[(methylimino)methyl]phenolate) and benzimidazole derivative (HL2 = (1H-benzo[d]imidazol-2-yl)methanolate), independently, at room temperature. A mixture of the two ligands, having similar coordination ability but different shapes, resulted in the butterfly-structured tetranuclear cobalt cluster [Co4(L1)2(L2)4](ClO4)2·2H2O (Co4). Electrospray Ionization Mass Spectrometry (ESI-MS) in real time revealed {Co1} → {Co2} → {Co4} → {Co7} stepwise assembly when single ligand is employed but the reaction with mixed ligands only gets to {Co4}, an intermediate of the former reactions...
March 5, 2018: Chemistry: a European Journal
Yan Liu, Yuanzhen Chen, Sai Li, Cheng Yong Shu, Yuan Fang, Bo Song
A three-dimensional Mn2 O3 @Co1.2 Ni1.8 O4 hybrid was synthesized via facile two-step processes and employed as a cathode catalyst in direct methanol fuel cell for the first time. Due to the unique architecture with ultra-thin and porous nanosheets of the Co1.2 Ni1.8 O4 shell, this composite exhibits better electrochemical performance than the pristine Mn2 O3 . Remarkably, it shows excellent methanol tolerance, even in a high concentration solution. The direct methanol fuel cell was assembled with Mn2 O3 @Co1...
March 1, 2018: ACS Applied Materials & Interfaces
P Annie Vinosha, Belina Xavier, S Krishnan, S Jerome Das
We report the influence of Co1-xZnxFe2O4 nanoferrites for the photodegradation of organic pollutant present in the aqueous solution. A series of Co1-xZnxFe2O4 (x = 0.01, 0.03, 0.05 and 0.10) nanoferrites have been synthesized via a facile and economically viable coprecipitation technique and investigated the effect of zinc dopant in their structural, optical, electrical and magnetic properties. X-ray diffraction and Fourier Transform Infrared spectral studies reveal the spinel phase formation of the as-synthesized nanoferrites...
August 1, 2018: Journal of Nanoscience and Nanotechnology
Sun-Ting Ma, Guo-Jie Ding, Xue-Wei Huang, Zi-Wei Wang, Li Wang, Mei-Ling Yu, Wen Shi, Yan-Ping Jiang, Li-Jie Tang, Yi-Gang Xu, Yi-Jing Li
PURPOSE: Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed to develop an effective oral vaccine for chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system. METHODOLOGY: Eight Lactobacillus strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability to assess their potential as a delivery vector...
January 19, 2018: Journal of Medical Microbiology
Yuanyuan Wen, Wei Meng, Chen Li, Lei Dai, Zhangxing He, Ling Wang, Ming Li, Jing Zhu
In this work, we demonstrate the synthesis and application of a novel Co II -based metal-organic framework {[Co 2 (Dcpp)(Bpe) 0.5 (H 2 O)(μ 2 -H 2 O)]·(Bpe) 0.5 } n (Co II -MOF, H 4 Dcpp = 4,5-bis(4'-carboxylphenyl)-phthalic acid, Bpe = 1,2-bis(4-pyridyl)ethane) as an electrochemical sensor for glucose detection. Single-crystal X-ray diffraction analysis shows that the Co II -MOF has a two-dimensional (2D) bilayer structure composed of Co 2 units and Dcpp 4- ligands. There are two kinds of Bpe in the structure: one serves as a bidentate ligand linking two Co1 atoms in each 2D layer; the other is just free in the lattice...
February 16, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
C Daniel Rivadeneira, Pablo J Venegas, Santiago R Ron
The genus Dendropsophus is one of the most speciose among Neotropical anurans and its number of described species is increasing. Herein, molecular, morphological, and bioacoustic evidence are combined to assess species limits within D. parviceps, a widely distributed species in the Amazon Basin. Phylogenetic relationships were assessed using 3040 bp sequences of mitochondrial DNA, genes 12S, ND1, and CO1. The phylogeny shows three well-supported clades. Bioacoustic and morphological divergence is congruent with those clades demonstrating that Dendropsophus parviceps is a species complex...
2018: ZooKeys
Sheng-Hua Ye, Zi-Xiao Shi, Jin-Xian Feng, Ye-Xiang Tong, Gao-Ren Li
Iron-substituted CoOOH porous nanosheet arrays grown on carbon fiber cloth (denoted as Fex Co1-x OOH PNSAs/CFC, 0≤x≤0.33) with 3D hierarchical structures are synthesized by in situ anodic oxidation of α-Co(OH)2 NSAs/CFC in solution of 0.01 m (NH4 )2 Fe(SO4 )2 . X-ray absorption fine spectra (XAFS) demonstrate that CoO6 octahedral structure in CoOOH can be partially substituted by FeO6 octahedrons during the transformation from α-Co(OH)2 to Fex Co1-x OOH, and this is confirmed for the first time in this study...
February 8, 2018: Angewandte Chemie
Annam Pavan-Kumar, Rajan Kumar, Pranali Pitale, Kang-Ning Shen, Philippe Borsa
The blue-spotted maskray, previously N. kuhlii, consists of up to eleven lineages representing separate species. Nine of these species (N. australiae, N. bobwardi, N. caeruleopunctata, N. malaccensis, N. moluccensis, N. orientale, N. vali, N. varidens, N. westpapuensis) have already been formally described and two (Indian Ocean maskray and Ryukyu maskray) remain undescribed. Here, the Indian Ocean maskray is described as a new species, Neotrygon indica sp. nov. Specimens of the new species were generally characterized on their dorsal side by a moderately large number of small ocellated blue spots, a low number of medium-sized ocellated blue spots, the absence of large ocellated blue spots, a high number of dark speckles, a few dark spots, and a conspicuous occipital mark...
February 4, 2018: Comptes Rendus Biologies
Sae-Hae Kim, Yu Na Kim, Ju Kim, Yong-Suk Jang
Mucosal vaccination is an ideal strategy to induce protective immunity in both mucosal and parenteral areas. Successful induction of an antigen-specific immune response via mucosal administration essentially requires the effective delivery of antigen into a mucosal immune inductive site, which depends on antigen delivery into M cells. We previously reported that M cells specifically express C5aR, and antigen targeting to C5aR by using specific ligands, including Co1 peptide, promotes the antigen-specific immune response in both mucosal and systemic immune compartments...
February 1, 2018: Cellular Immunology
Nathaniel Evans
Portunoidea is a diverse lineage of ecologically and economically important marine crabs comprising 8 families and 14 subfamilies. Closely related portunid subfamilies Caphyrinae and Thalamitinae constitute some of this group's greatest morphological and taxonomic diversity, and are the only known lineages to include symbiotic taxa. Emergence of symbiosis in decapods remains poorly studied and portunoid crabs provide an interesting, but often overlooked example. Yet the paucity of molecular phylogenetic data available for Portunoidea makes it challenging to investigate the evolution and systematics of the group...
2018: PeerJ
Julia F Hopkins, Robert E Denroche, Jennifer A Aguiar, Faiyaz Notta, Ashton A Connor, Julie M Wilson, Lincoln D Stein, Steven Gallinger, Paul C Boutros
Somatic mutations have been found in the mitochondria in different types of cancer cells, but it is not clear whether these affect tumorigenesis or tumor progression. We analyzed mitochondrial genomes of 268 early-stage, resected pancreatic ductal adenocarcinoma tissues and paired non-tumor tissues. We defined a mitochondrial somatic mutation (mtSNV) as a position where the difference in heteroplasmy fraction between tumor and normal sample was 0.2 or more. Our analysis identified 304 mtSNVs, with at least 1 mtSNV in 61% (164/268) of tumor samples...
January 25, 2018: Gastroenterology
Caifu Dong, Lijun Guo, Yanyan He, Limei Shang, Yitai Qian, Liqiang Xu
Cobalt sulfides are attractive as intriguing candidates for anodes in SIBs and LIBs owing to their unique chemical and physical properties. In this study, a precursor of Co1-xS with a uniform and hollow nanospherical architecture is obtained with a high yield via a mild solvothermal method in the presence of 2-methylimidazole at first. Then, Co1-xS, Co1-xS/C (ultrafine Co1-xS nanoparticles embedded in the shells of the nitrogen-doped porous carbon hollow nanosphere), and Co1-xS@C (Co1-xS nanoparticles entirely covered by an external amorphous carbon layer) were selectively fabricated via direct calcination or PPy coating & calcination of the obtained precursor...
January 23, 2018: Nanoscale
Naim Saglam, Ralph Saunders, Shirley A Lang, Daniel H Shain
Myzobdella lugubris is a commensal leech on crustaceans and a parasite to fishes, surviving predominantly in brackish waters throughout North America. Specimens in this study were collected within the tidal zone of the Delaware River basin (New Jersey and Pennsylvania). To compare regional M. lugubris specimens, defined characters were scored after dorsal and ventral dissections, and phylogenetic relationships were resolved using cytochrome c oxidase subunit 1 (CO1), 12S ribosomal RNA (rDNA) and 18S rDNA gene fragments...
March 26, 2018: Acta Parasitologica
Anna Pavlova, Jerry M Parks, James C Gumbart
Corrinoid cofactors such as cobalamin are used by many enzymes and are essential for most living organisms. Therefore, there is broad interest in investigating cobalamin-protein interactions with molecular dynamics simulations. Previously developed parameters for cobalamins are based mainly on crystal structure data. Here, we report CHARMM-compatible force field parameters for several corrinoids developed from quantum mechanical calculations. We provide parameters for corrinoids in three oxidation states, Co3+, Co2+ and Co1+, and with various axial ligands...
January 15, 2018: Journal of Chemical Theory and Computation
Haroon Ahmed, Sérgio Ramalho Sousa, Sami Simsek, Sofia Anastácio, Seyma Gunyakti Kilinc
Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis...
December 2017: Korean Journal of Parasitology
Valérie Benhammou, Roland Tubiana, Sophie Matheron, Pierre Sellier, Laurent Mandelbrot, Jérôme LE Chenadec, Emmanuelle Marel, Babak Khoshnood, Josiane Warszawski
BACKGROUND: Chronic HBV or HCV infection is frequent in HIV- infected persons but their impact on pregnant HIV-infected women is understudied. We explored whether these coinfections are associated with adverse pregnancy outcomes and lower response to antiretroviral therapy (ART). METHODS: Pregnancies in HIV-1-infected women included in the ANRS French Perinatal Cohort between 2005 and 2013 were analyzed if HBV and HCV infection statuses were available. RESULTS: Among 4236 women, the prevalence of HBV (HBs Ag+) and HCV (RNA+) were 6...
December 27, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
David A Boyd, Patchara Nithirojpakdee, Gridsada Deein, Chavalit Vidthayanon, Chaiwut Grudpan, Weerapongse Tangjitjaroen, John M Pfeiffer, Zachary S Randall, Tippamas Srisombat, Lawrence M Page
Acantopsis (Cobitidae) is revised based on analysis of morphological and molecular data. Four of the six available names, A. dialuzona, A. spectabilis, A. octoactinotos, and A. thiemmedhi, are valid, and three new species, A. rungthipae, A. dinema, and A. ioa, are described. All species are described morphologically, distributions are mapped, and relationships are discussed for those for which molecular data (CO1, RAG1) are available. Labial barbels, color pattern, and meristic counts are the most diagnostic features...
October 31, 2017: Zootaxa
Kareen E Schnabel, Ingo Burghardt, Shane T Ahyong
Squat lobsters have only recently been recorded from the Macquarie Ridge, which extends south between New Zealand and Antarctica. Among these, Uroptychus insignis (Henderson, 1885) was recorded for the first time outside the western Indian Ocean, exhibiting only subtle morphological differences. Reexamination of the Macquarie Ridge and Indian Ocean specimens attributed to U. insignis using morphological and molecular data revealed the Macquarie Ridge form to represent a separate species. Subtle but consistent morphological differences are evident and partial CO1 sequence data indicates that the specimens collected on Macquarie Ridge differ from those collected in the Indian Ocean by more than 7%...
November 23, 2017: Zootaxa
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