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Endothelial colony forming cell

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https://www.readbyqxmd.com/read/29908162/isolation-and-characterization-of-endothelial-colony-forming-cells-from-mononuclear-cells-of-rat-bone-marrow
#1
Shukui Yu, Zhongxuan Li, Wei Zhang, Zhengde Du, Ke Liu, Denghua Yang, Shusheng Gong
Transplantation of bone marrow-derived endothelial progenitor cells (BM-EPCs) has been used as a therapeutic strategy for vascular repair. However, it remains controversial whether BM-EPCs exhibit clonal endothelial colony-forming cell (ECFC) capacity, a characteristic of true EPCs. The aim of this study was to isolate and explore the cellular properties of BM-ECFCs. We isolated BM-ECFCs from rat bone marrow with high purity via an optimized method. This approach involved the removal of selective colonies based on the conventional differential adhesive culture method used to isolate ECFCs from peripheral and umbilical cord blood...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29895317/hypoxia-with-wharton-s-jelly-mesenchymal-stem-cell-coculture-maintains-stemness-of-umbilical-cord-blood-derived-cd34-cells
#2
Dewan Zhao, Lingjia Liu, Qiang Chen, Fangfang Wang, Qiuyang Li, Qiang Zeng, Jingcao Huang, Maowen Luo, Wenxian Li, Yuhuan Zheng, Ting Liu
BACKGROUND: The physiological approach suggests that an environment associating mesenchymal stromal cells with low O2 concentration would be most favorable for the maintenance of hematopoietic stem/progenitor cells (HSPCs). To test this hypothesis, we performed a coculture of cord blood CD34+ cells with Wharton's jelly mesenchymal stem cells (WJ-MSCs) under different O2 concentration to simulate the growth of HSPCs in vivo, and assessed the impacts on stemness maintenance and proliferation of cord blood HSPCs in vitro...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29883579/endothelial-colony-forming-cell-derived-exosomes-restore-blood-brain-barrier-continuity-in-mice-subjected-to-traumatic-brain-injury
#3
Weiwei Gao, Fei Li, Li Liu, Xin Xu, Baoliang Zhang, Yingang Wu, Dongpei Yin, Shuai Zhou, Dongdong Sun, Ying Huang, Jianning Zhang
Traumatic brain injury (TBI) tends to cause disruption of the blood-brain barrier (BBB). Previous studies have shown that intravenously or intracerebroventricularly infused human umbilical cord blood-derived endothelial colony-forming cells (ECFCs) can home to injury sites and improve outcomes in mice subjected to experimental TBI. Several reports have demonstrated that these cells did not incorporate directly into newly formed vasculature but instead stimulated the proliferation and migration of tissue-resident endothelial cells (ECs) via paracrine mechanisms...
June 5, 2018: Experimental Neurology
https://www.readbyqxmd.com/read/29873804/engineering-of-a-complex-bone-tissue-model-with-endothelialised-channels-and-capillary-like-networks
#4
B J Klotz, K S Lim, Y X Chang, B J Soliman, I Pennings, F P Melchels, F P Melchels, A J Rosenberg, J Malda, D Gawlittal
In engineering of tissue analogues, upscaling to clinically-relevant sized constructs remains a significant challenge. The successful integration of a vascular network throughout the engineered tissue is anticipated to overcome the lack of nutrient and oxygen supply to residing cells. This work aimed at developing a multiscale bone-tissue-specific vascularisation strategy. Engineering pre-vascularised bone leads to biological and fabrication dilemmas. To fabricate channels endowed with an endothelium and suitable for osteogenesis, rather stiff materials are preferable, while capillarisation requires soft matrices...
May 30, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29788448/inhibition-of-endothelial-progenitor-cells-may-explain-the-high-cardiovascular-event-rate-in-patients-with-rheumatoid-arthritis
#5
Mohamad Adawi, Nina Pastuck, Golan Saaida, Rizak Sirchan, Abdalla Watad, Arnon Blum
Background: Rheumatoid arthritis (RA) patients may suffer cardiovascular (CV) events much more than the general population, and CV disease is the leading cause of death in patients with RA. Our hypothesis was that impaired function of endothelial progenitor cells may contribute to endothelial dysfunction and the clinical CV events of patients with RA. Methods: 27 RA patients (9 males and 18 females) with an active disease and 13 healthy subjects who served as the control group (9 males and 4 females) were enrolled to this prospective study...
May 16, 2018: QJM: Monthly Journal of the Association of Physicians
https://www.readbyqxmd.com/read/29777301/role-of-the-nrp-1-mediated-vegfr2-independent-pathway-on-radiation-sensitivity-of-non-small-cell-lung-cancer-cells
#6
Chenxi Hu, Panrong Zhu, Youyou Xia, Kaiyuan Hui, Mei Wang, Xiaodong Jiang
PURPOSE: To determine if inhibiting neuropilin-1 (NRP-1) affects the radiosensitivity of NSCLC cells through a vascular endothelial growth factor receptor 2 (VEGFR2)-independent pathway, and to assess the underlying mechanisms. METHODS: The expression of VEGFR2, NRP-1, related signaling molecules, abelson murine leukemia viral oncogene homolog 1 (ABL-1), and RAD51 were determined by RT-PCR and Western blotting, respectively. Radiosensitivity was assessed using the colony-forming assay, and the cell apoptosis were analyzed by flow cytometry...
May 17, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29776986/the-characteristics-of-vessel-lining-cells-in-normal-spleens-and-their-role-in-the-pathobiology-of-myelofibrosis
#7
Jiajing Qiu, Mohamed E Salama, Cing Siang Hu, Yan Li, Xiaoli Wang, Ronald Hoffman
The CD34- CD8α+ , sinusoid lining, littoral cells (LCs), and CD34+ CD8α- , splenic vascular endothelial cells (SVECs) represent 2 distinct cellular types that line the vessels within normal spleens and those of patients with myelofibrosis (MF). To further understand the respective roles of LCs and SVECs, each was purified from normal and MF spleens, cultured, and characterized. Gene expression profiling indicated that LCs were a specialized type of SVEC. LCs possessed a distinct gene expression profile associated with cytoskeleton regulation, cellular interactions, endocytosis, and iron transport...
May 22, 2018: Blood Advances
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#8
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29737200/human-peripheral-blood-mononuclear-cells-incubated-in-vasculogenic-conditioning-medium-dramatically-improve-ischemia-reperfusion-acute-kidney-injury-in-mice
#9
Takayasu Ohtake, Shuzo Kobayashi, Shimon Slavin, Yasuhiro Mochida, Kunihiro Ishioka, Hidekazu Moriya, Sumi Hidaka, Ryo Matsuura, Maki Sumida, Daisuke Katagiri, Eisei Noiri, Kayoko Okada, Hiroshi Mizuno, Rica Tanaka
Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mice aged 7 to 8 wk. PBMNCs were isolated from healthy volunteers and were subjected to quality and quantity controlled (QQc) culture for 7 d in medium containing stem cell factor, thrombopoietin, Flt-3 ligand, vascular endothelial growth factor, and interleukin 6...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29734169/cell-based-therapies-in-acute-kidney-injury-aki
#10
Daniel Patschan, Ivo Buschmann, Oliver Ritter, Andreas Kribben
Acute kidney injury frequently occurs in hospitalized patients all over the world. The prognosis remains poor since specific therapies for promoting kidney regeneration/repair are still missing. In recent years cell-based strategies have improved AKI outcomes under experimental circumstances. Four groups of cells, each of them displaying certain biological and functional characteristics have been evaluated in AKI, induced Pluripotent Stem Cells (iPSCs), Spermatagonial Stem Cells (SSCs), Proangiogenic Cells (PACs) and Endothelial Colony Forming Cells (ECFCs), and Mesenchymal Stem Cells (MSCs)...
May 3, 2018: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29720269/acidic-preconditioning-of-endothelial-colony-forming-cells-ecfc-promote-vasculogenesis-under-proinflammatory-and-high-glucose-conditions-in-vitro-and-in-vivo
#11
Hebe Agustina Mena, Paula Romina Zubiry, Blandine Dizier, Mirta Schattner, Catherine Boisson-Vidal, Soledad Negrotto
BACKGROUND: We have previously demonstrated that acidic preconditioning of human endothelial colony-forming cells (ECFC) increased proliferation, migration, and tubulogenesis in vitro, and increased their regenerative potential in a murine model of hind limb ischemia without baseline disease. We now analyze whether this strategy is also effective under adverse conditions for vasculogenesis, such as the presence of ischemia-related toxic molecules or diabetes, one of the main target diseases for cell therapy due to their well-known healing impairments...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29615071/mature-and-progenitor-endothelial-cells-perform-angiogenesis-also-under-protease-inhibition-the-amoeboid-angiogenesis
#12
Anastasia Chillà, Francesca Margheri, Alessio Biagioni, Mario Del Rosso, Gabriella Fibbi, Anna Laurenzana
BACKGROUND: Controlling vascular growth is a challenging aim for the inhibition of tumor growth and metastasis. The amoeboid and mesenchymal types of invasiveness are two modes of migration interchangeable in cancer cells: the Rac-dependent mesenchymal migration requires the activity of proteases; the Rho-ROCK-dependent amoeboid motility is protease-independent and has never been described in endothelial cells. METHODS: A cocktail of physiologic inhibitors (Ph-C) of serine-proteases, metallo-proteases and cysteine-proteases, mimicking the physiological environment that cells encounter during their migration within the angiogenesis sites was used to induce amoeboid style migration of Endothelial colony forming cells (ECFCs) and mature endothelial cells (ECs)...
April 3, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29603903/photobiomodulation-of-freshly-isolated-human-adipose-tissue-derived-stromal-vascular-fraction-cells-by-pulsed-light-emitting-diodes-for-direct-clinical-application
#13
E Priglinger, J Maier, S Chaudary, C Lindner, C Wurzer, S Rieger, H Redl, S Wolbank, P Dungel
A highly interesting source for adult stem cells is adipose tissue, from which the stromal vascular fraction (SVF)-a heterogeneous cell population including the adipose-derived stromal/stem cells-can be obtained. To enhance the regenerative potential of freshly isolated SVF cells, low-level light therapy (LLLT) was used. The effects of pulsed blue (475 nm), green (516 nm), and red (635 nm) light from light-emitting diodes applied on freshly isolated SVF were analysed regarding cell phenotype, cell number, viability, adenosine triphosphate content, cytotoxicity, and proliferation but also osteogenic, adipogenic, and proangiogenic differentiation potential...
March 31, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29583085/functional-heterogeneity-of-endothelial-cells-derived-from-human-pluripotent-stem-cells
#14
Saritha S D'Souza, Akhilesh Kumar, Igor I Slukvin
Specification of endothelial cells (ECs) into arterial, venous, and lymphatic cells is a crucial process of vascular development, and expanding our knowledge about EC specification from human pluripotent stem cells (hPSCs) will aid the design of optimal strategies for producing desired types of ECs for therapies. In our prior studies, we revealed that hPSC-derived VE-cadherin(V)+ CD31+ CD34+ ECs are heterogeneous and include at least three major subsets with distinct hemogenic properties: V+ CD43/235a- CD73- hemogenic endothelial progenitors (HEPs), V+ CD43lo CD235a+ 73- angiogenic hematopoietic progenitors (AHPs), and V+ CD43/235a- 73+ non-HEPs...
April 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29566115/mir-31-regulates-the-function-of-diabetic-endothelial-progenitor-cells-by-targeting-satb2
#15
Weishuai Lian, Xiaoxiao Hu, Rongfeng Shi, Shilong Han, Chuanwu Cao, Kun Wang, Maoquan Li
Endothelial malfunctions in patients with diabetes are known to result in vascular diseases, and endothelial progenitor cells (EPCs) are indispensable for the functional preservation of the vascular endothelium. MicroRNA-31 (miR-31) has been found to be able to modulate the differentiation of stem cells. However, it is still unclear how miR-31 functions in diabetic EPCs. The aim of this study was to investigate how miR-31 regulates diabetic EPC function. In the current study, miR-31 expression was compared between normal and diabetic EPCs...
April 1, 2018: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29549430/cells-with-hematopoietic-potential-reside-within-mouse-proepicardium
#16
Ewa Jankowska-Steifer, Justyna Niderla-Bielińska, Bogdan Ciszek, Marek Kujawa, Mateusz Bartkowiak, Aleksandra Flaht-Zabost, Daria Klosinska, Anna Ratajska
During embryonic development, hematopoietic cells are present in areas of blood-vessel differentiation. These hematopoietic cells emerge from a specific subpopulation of endothelial cells called the hemogenic endothelium. We have previously found that mouse proepicardium contained its own population of endothelial cells forming a network of vascular tubules. We hypothesize that this EC population contains cells of hematopoietic potential. Therefore, we investigated an in vitro hematopoietic potential of proepicardial cell populations...
March 16, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29538431/mycophenolic-acid-induces-senescence-of-vascular-precursor-cells
#17
Ellen Go, Stefan P Tarnawsky, W Chris Shelley, Kimihiko Banno, Yang Lin, Chang-Hyun Gil, Emily K Blue, Laura S Haneline, Kathleen M O'Neil, Mervin C Yoder
OBJECTIVE: Endothelial dysfunction is central to the pathogenesis of many rheumatic diseases, typified by vascular inflammation and damage. Immunosuppressive drugs induce disease remission and lead to improved patient survival. However, there remains a higher incidence of cardiovascular disease in these patients even after adequate disease control. The purpose of this study was to determine the effect of mycophenolic acid (MPA), a commonly used immunosuppressive drug in rheumatology, on blood vessel or circulating endothelial colony forming cell number and function...
2018: PloS One
https://www.readbyqxmd.com/read/29473177/manufacture-of-endothelial-colony-forming-progenitor-cells-from-steady-state-peripheral-blood-leukapheresis-using-pooled-human-platelet-lysate
#18
Georg Siegel, Erika Fleck, Stefanie Elser, Ursula Hermanutz-Klein, Marc Waidmann, Hinnak Northoff, Erhard Seifried, Richard Schäfer
BACKGROUND: Endothelial colony-forming progenitor cells (ECFCs) are promising candidates for cell therapies. However, ECFC translation to the clinic requires optimized isolation and manufacture technologies according to good manufacturing practice (GMP). STUDY DESIGN AND METHODS: ECFCs were manufactured from steady-state peripheral blood (PB) leukapheresis (11 donors), using GMP-compliant technologies including pooled human platelet (PLT) lysate, and compared to human umbilical cord endothelial cells, human aortic endothelial cells, and human cerebral microvascular endothelial cells...
May 2018: Transfusion
https://www.readbyqxmd.com/read/29443032/kinetic-analysis-of-vasculogenesis-quantifies-dynamics-of-vasculogenesis-and-angiogenesis-in-vitro
#19
Kaela M Varberg, Seth Winfree, Kenneth W Dunn, Laura S Haneline
Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis has become a central readout of endothelial progenitor cell functionality, and therefore, several attempts have been made to improve both in vitro and in vivo vasculogenesis models. However, standard methods are limited in scope, with static measurements failing to capture many aspects of this highly dynamic process. Therefore, the goal of developing this novel protocol was to assess the kinetics of in vitro vasculogenesis in order to quantitate rates of network formation and stabilization, as well as provide insight into potential mechanisms underlying vascular dysfunction...
January 31, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29429943/cd157-marks-tissue-resident-endothelial-stem-cells-with-homeostatic-and-regenerative-properties
#20
Taku Wakabayashi, Hisamichi Naito, Jun-Ichi Suehiro, Yang Lin, Hideya Kawaji, Tomohiro Iba, Tsukasa Kouno, Sachi Ishikawa-Kato, Masaaki Furuno, Kazuhiro Takara, Fumitaka Muramatsu, Jia Weizhen, Hiroyasu Kidoya, Katsuhiko Ishihara, Yoshihide Hayashizaki, Kohji Nishida, Mervin C Yoder, Nobuyuki Takakura
The generation of new blood vessels via angiogenesis is critical for meeting tissue oxygen demands. A role for adult stem cells in this process remains unclear. Here, we identified CD157 (bst1, bone marrow stromal antigen 1) as a marker of tissue-resident vascular endothelial stem cells (VESCs) in large arteries and veins of numerous mouse organs. Single CD157+ VESCs form colonies in vitro and generate donor-derived portal vein, sinusoids, and central vein endothelial cells upon transplantation in the liver...
March 1, 2018: Cell Stem Cell
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