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Endothelial colony forming cell

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https://www.readbyqxmd.com/read/29033304/epigenetic-activation-of-pro-angiogenic-signaling-pathways-in-human-endothelial-progenitors-increases-vasculogenesis
#1
Sylvain Fraineau, Carmen G Palii, Brian McNeill, Morten Ritso, William C Shelley, Nutan Prasain, Alphonse Chu, Elodie Vion, Kristy Rieck, Sharmin Nilufar, Theodore J Perkins, Michael A Rudnicki, David S Allan, Mervin C Yoder, Erik J Suuronen, Marjorie Brand
Human endothelial colony-forming cells (ECFCs) represent a promising source of adult stem cells for vascular repair, yet their regenerative capacity is limited. Here, we set out to understand the molecular mechanism restricting the repair function of ECFCs. We found that key pro-angiogenic pathways are repressed in ECFCs due to the presence of bivalent (H3K27me3/H3K4me3) epigenetic marks, which decreases the cells' regenerative potential. Importantly, ex vivo treatment with a combination of epigenetic drugs that resolves bivalent marks toward the transcriptionally active H3K4me3 state leads to the simultaneous activation of multiple pro-angiogenic signaling pathways (VEGFR, CXCR4, WNT, NOTCH, SHH)...
October 11, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29030959/ipsc-derived-vascular-cell-spheroids-as-building-blocks-for-scaffold-free-biofabrication
#2
Leni Moldovan, April Barnard, Chang-Hyun Gil, Y Lin, Maria B Grant, Mervin C Yoder, Nutan Prasain, Nicanor I Moldovan
Recently a protocol was established to obtain large quantities of human induced pluripotent stem cells (iPSC)-derived endothelial progenitors, called endothelial colony forming cells (ECFC), and of candidate smooth-muscle forming cells (SMFC). Here, we tested their suitability for assembling in spheroids, and in larger 3D cell constructs. iPSC-derived ECFC and SMFC were labeled with tdTomato and eGFP, respectively. Spheroids were formed in ultra-low adhesive wells, and their dynamic proprieties were studied by time-lapse microscopy, or by confocal microscopy...
October 14, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29030540/hypoxia-induced-activation-of-jak-stat3-signaling-pathway-promotes-trophoblast-cell-viability-and-angiogenesis-in-preeclampsia
#3
Chengfang Xu, Xuejiao Li, Peiling Guo, Jia Wang
BACKGROUND To explore the effect of hypoxic preconditioning on the JAK/STAT3 signaling pathway and its effect on trophoblast cell viability and angiogenesis in preeclampsia (PE). MATERIAL AND METHODS Placental tissues from normal pregnant women and PE patients were collected to detect the expression levels of JAK and STAT3. Trophoblast cells separated from the PE patients were assigned to 4 groups. The expression levels of phosphorylated p-JAK and p-STAT3 were measured by Western blot. Cell viability, colony-forming ability, and cell apoptosis were assessed...
October 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29025705/differentiation-evaluation-and-application-of-human-induced-pluripotent-stem-cell-derived-endothelial-cells
#4
REVIEW
Yang Lin, Chang-Hyun Gil, Mervin C Yoder
The emergence of induced pluripotent stem cell (iPSC) technology paves the way to generate large numbers of patient-specific endothelial cells (ECs) that can be potentially delivered for regenerative medicine in patients with cardiovascular disease. In the last decade, numerous protocols that differentiate EC from iPSC have been developed by many groups. In this review, we will discuss several common strategies that have been optimized for human iPSC-EC differentiation and subsequent studies that have evaluated the potential of human iPSC-EC as a cell therapy or as a tool in disease modeling...
October 12, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29021911/interval-exercise-increases-angiogenic-cell-function-in-postmenopausal-women
#5
Emma Harris, Mark Rakobowchuk, Karen M Birch
INTRODUCTION: Exercise can help to negate the increased cardiovascular disease risk observed in women after the menopausal transition. This study sought to determine whether interval or continuous exercise has differential effects on endothelial function and circulating angiogenic cell (CAC) number and function in postmenopausal women. METHODS: Fifteen healthy postmenopausal women completed a 30 min acute moderate-intensity continuous (CON) and interval exercise (MOD-INT) session on a cycle ergometer on separate days...
2017: BMJ Open Sport & Exercise Medicine
https://www.readbyqxmd.com/read/29020504/fibrocartilage-stem-cells-engraft-and-self-organize-into-vascularized-bone
#6
J Nathan, A Ruscitto, S Pylawka, A Soharby, C J Shawber, M C Embree
Angiogenesis is a complex, multicellular process that is critical for bone development and generation. Endochondral ossification depends on an avascular cartilage template that completely remodels into vascularized bone and involves a dynamic interplay among chondrocytes, osteoblasts, and endothelial cells. We have discovered fibrocartilage stem cells (FCSCs) derived from the temporomandibular joint (TMJ) mandibular condyle that generates cartilage anlagen, which is subsequently remodeled into vascularized bone using an ectopic transplantation model...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28980775/long-term-recovery-after-endothelial-colony-forming-cells-or-human-umbilical-cord-blood-cells-administration-in-a-rat-model-of-neonatal-hypoxic-ischemic-encephalopathy
#7
Isabelle Grandvuillemin, Philippe Garrigue, Alaa Ramdani, Farid Boubred, Umberto Simeoni, Françoise Dignat-George, Florence Sabatier, Benjamin Guillet
Neonatal hypoxic-ischemic encephalopathy (NHIE) is a dramatic perinatal complication, associated with poor neurological prognosis despite neuroprotection by therapeutic hypothermia, in the absence of an available curative therapy. We evaluated and compared ready-to-use human umbilical cord blood cells (HUCBC) and bankable but allogeneic endothelial progenitors (ECFC) as cell therapy candidate for NHIE. We compared benefits of HUCBC and ECFC transplantation 48 hours after injury in male rat NHIE model, based on the Rice-Vannucci approach...
October 5, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28980146/egfl7-represses-the-vasculogenic-potential-of-human-endothelial-progenitor-cells
#8
Clément d'Audigier, Sophie Susen, Adeline Blandinieres, Virginie Mattot, Bruno Saubamea, Elisa Rossi, Nathalie Nevo, Séverine Lecourt, Coralie L Guerin, Blandine Dizier, Nicolas Gendron, Bertrand Caetano, Pascale Gaussem, Fabrice Soncin, David M Smadja
Egfl7 (VE-statin) is a secreted protein mostly specific to the endothelial lineage during development and in the adult and which expression is enhanced during angiogenesis. Egfl7 involvement in human postnatal vasculogenesis remains unresolved yet. Our aim was to assess Egfl7 expression in several angiogenic cell types originating from human bone marrow, peripheral blood, or cord blood. We found that only endothelial colony forming cells (ECFC), which are currently considered as the genuine endothelial precursor cells, expressed large amounts of Egfl7...
October 4, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28978525/ampk-klf2-signaling-pathway-mediates-the-proangiogenic-effect-of-erythropoietin-in-endothelial-colony-forming-cells
#9
Dawei Wang, Yimeng Song, Jianshu Zhang, Wei Pang, Xian Wang, Yi Zhu, Xiaoxia Li
Endothelial colony forming cells (ECFCs) were proved to take part in post-natal vasculogenesis and injury repair. The angiogenic properties of ECFCs could be influenced by various cytokines, chemokines, and growth factors. Erythropoietin (EPO) is a promising cytokine participating in angiogenesis. However, the mechanisms for EPO's proangiogenic effect still remain largely elusive. Here, we investigated the role of AMP-activated protein kinase (AMPK)-Krüppel-like factor 2 (KLF2) signaling pathway in the proangiogenic effect of EPO in ECFCs...
October 4, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28967639/tissue-engineered-bone-for-treatment-of-combat-related-limb-injuries
#10
R G Vasyliev, V M Oksymets, A E Rodnichenko, A V Zlatska, O S Gubar, I M Gordiienko, D O Zubov
AIM: Based on our preliminary positive clinical results with use of cultured bone marrow-derived multipotent mesenchymal stem/stromal cells in traumatology, our aim was to develop living three-dimensional tissue-engineered bone equivalent transplantation technology for restoration of critical sized bone defects caused by combat related high energy trauma. MATERIALS AND METHODS: To fabricate bone equivalent we used devitalized allogeneic bone scaffolds (blocks and chips) seeded with cultured autologous cells: bone marrow-derived multipotent mesenchymal stem/stromal cells in mix with periosteal progenitor cells and endothelial progenitor cells...
September 2017: Experimental Oncology
https://www.readbyqxmd.com/read/28964937/hypoxia-induces-the-dysfunction-of-human-endothelial-colony-forming-cells-via-hif-1%C3%AE-signaling
#11
Mengyu He, Shuying Ma, Qing Cai, Yan Wu, Chengjie Shao, Hui Kong, Hong Wang, Xiaoning Zeng, Weiping Xie
Endothelial injury is considered as a trigger of pulmonary vascular lesions in the pathogenesis of hypoxic pulmonary hypertension (HPH). Although endothelial colony-forming cells (ECFCs) have vascular regeneration potential to maintain endothelial integrity, hypoxia-induced precise alteration in ECFCs function remains controversial. This study investigated the impact of hypoxia on human ECFCs function in vitro and the underlying mechanism. We found that hypoxia inhibited ECFCs proliferation, migration and angiogenesis...
September 28, 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28951723/microvesicles-derived-from-indoxyl-sulfate-treated-endothelial-cells-induce-endothelial-progenitor-cells-dysfunction
#12
Andres Carmona, Fatima Guerrero, Paula Buendia, Teresa Obrero, Pedro Aljama, Julia Carracedo
Cardiovascular disease is a major cause of mortality in chronic kidney disease patients. Indoxyl sulfate (IS) is a typical protein-bound uremic toxin that cannot be effectively cleared by conventional dialysis. Increased IS is associated with the progression of chronic kidney disease and development of cardiovascular disease. After endothelial activation by IS, cells release endothelial microvesicles (EMV) that can induce endothelial dysfunction. We developed an in vitro model of endothelial damage mediated by IS to evaluate the functional effect of EMV on the endothelial repair process developed by endothelial progenitor cells (EPCs)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28940125/responses-of-an-american-eel-brain-endothelial-like-cell-line-to-selenium-deprivation-and-to-selenite-selenate-and-selenomethionine-additions-in-different-exposure-media
#13
Sophia R Bloch, John J Kim, Phuc H Pham, Peter V Hodson, Lucy E J Lee, Niels C Bols
The effect of selenium deprivation and addition on the American eel brain endothelial cell line (eelB) was studied in three exposure media: complete growth medium (L15/FBS), serum-free medium (L15), and minimal medium (L15/ex). L15/ex contains only galactose and pyruvate and allowed the deprivation of selenium on cells to be studied. In L15/ex, without any obvious source of selenium, eelB cells survived for at least 7 d, formed capillary-like structures (CLS) on Matrigel, and migrated to heal wounds. Three selenium compounds were added to cultures: selenite, selenate, and selenomethionine (SeMet)...
September 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28915234/tissue-regeneration-using-endothelial-colony-forming-cells-promising-cells-for-vascular-repair
#14
REVIEW
Kimihiko Banno, Mervin C Yoder
To repair and rebuild damaged tissue in diseased human subjects remains a daunting challenge for clinical medicine. Proper vascular formation that serves to deliver blood-borne nutrients and adequate levels of oxygen and remove wastes is critical for successful tissue regeneration. Endothelial colony-forming cells (ECFC) represent a promising cell source for revascularization of damaged tissue. ECFC are identified by displaying a hierarchy of clonal proliferative potential and by pronounced postnatal vascularization ability in vivo...
September 15, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28910333/reduced-proliferation-of-endothelial-colony-forming-cells-in-unprovoked-venous-thromboembolic-disease-as-a-consequence-of-endothelial-dysfunction
#15
Rubicel Hernandez-Lopez, Antonieta Chavez-Gonzalez, Patricia Torres-Barrera, Dafne Moreno-Lorenzana, Norma Lopez-DiazGuerrero, David Santiago-German, Irma Isordia-Salas, David Smadja, Mervin C Yoder, Abraham Majluf-Cruz, J Antonio Alvarado-Moreno
BACKGROUND: Venous thromboembolic disease (VTD) is a public health problem. We recently reported that endothelial colony-forming cells (ECFCs) derived from endothelial cells (EC) (ECFC-ECs) from patients with VTD have a dysfunctional state. For this study, we proposed that a dysfunctional status of these cells generates a reduction of its proliferative ability, which is also associated with senescence and reactive oxygen species (ROS). METHODS AND RESULTS: Human mononuclear cells (MNCs) were obtained from peripheral blood from 40 healthy human volunteers (controls) and 50 patients with VTD matched by age (20-50 years) and sex to obtain ECFCs...
2017: PloS One
https://www.readbyqxmd.com/read/28901450/role-of-hypoxia%C3%A2-mediated-cellular-prion-protein-functional-change-in-stem-cells-and-potential-application-in-angiogenesis-review
#16
Seung Pil Yun, Yong-Seok Han, Jun Hee Lee, Yeo Min Yoon, Chul Won Yun, Peter Rhee, Sang Hun Lee
Cellular prion protein (PrPC) can replace other pivotal molecules due to its interaction with several partners in performing a variety of important biological functions that may differ between embryonic and mature stem cells. Recent studies have revealed major advances in elucidating the putative role of PrPC in the regulation of stem cells and its application in stem cell therapy. What is special about PrPC is that its expression may be regulated by hypoxia‑inducible factor (HIF)‑1α, which is the transcriptional factor of cellular response to hypoxia...
August 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28881615/perfluorodecanoic-acid-pfda-promotes-gastric-cell-proliferation-via-spla2-iia
#17
Tianyi Dong, Yanping Peng, Ning Zhong, Fengyan Liu, Hanyu Zhang, Mengchen Xu, Rutao Liu, Mingyong Han, Xingsong Tian, Jihui Jia, Lap Kam Chang, Liang-Hong Guo, Shili Liu
The association of perfluorodecanoicacid (PFDA) with tumor promotion and associated effects is not clear. Given that PDFA is mostly consumed with food and drinking water, we evaluated the effects of PFDA on a gastric cell line. When added to cell cultures, PFDA significantly increased growth rate and colony forming ability compared with control treatment. We found that suppression of cell senescence, but not apoptosis or autophagy was associated with PFDA-induced promotion of cell amount. To determine the molecular mechanism that was involved, DNA microarray assays was used to analyze changes in gene expression in response to PFDA treatment...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28862380/fetal-bone-marrow-derived-mesenchymal-stem-stromal-cells-enhance-humanization-and-bone-formation-of-bmp7-loaded-scaffolds
#18
Abbas Shafiee, Jeremy G Baldwin, Jatin Patel, Boris M Holzapfel, Nicholas M Fisk, Kiarash Khosrotehrani, Dietmar W Hutmacher
Tissue engineered constructs built with human cells capable of generating a bone-like organ within the mouse have attracted considerable interest over the past decade. Here, we aimed to compare the utility of human mesenchymal stem/stromal cells (MSC) isolated from fetal term placenta (fPL-MSC) and fetal first trimester bone marrow (fBM-MSC) in a polycaprolactone scaffold/BMP7-based model in nude mice. Furthermore, fPL-MSC were co-seeded with fetal placenta-derived endothelial colony forming cells (ECFC) to assess the impact of ECFC on fPL-MSC osteogenesis...
September 1, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28816358/trpc3-mediated-ca-2-signals-as-a-promising-strategy-to-boost-therapeutic-angiogenesis-in-failing-hearts-the-role-of-autologous-endothelial-colony-forming-cells
#19
REVIEW
Francesco Moccia, Angela Lucariello, Germano Guerra
Endothelial progenitor cells (EPCs) are a sub-population of bone marrow-derived mononuclear cells that are released in circulation to restore damaged endothelium during its physiological turnover or rescue blood perfusion after an ischemic insult. Additionally, they may be mobilized from perivascular niches located within larger arteries' wall in response to hypoxic conditions. For this reason, EPCs have been regarded as an effective tool to promote revascularization and functional recovery of ischemic hearts, but clinical application failed to exploit the full potential of patients-derived cells...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28811647/biogenesis-of-pro-senescent-microparticles-by-endothelial-colony-forming-cells-from-premature-neonates-is-driven-by-sirt1-dependent-epigenetic-regulation-of-mkk6
#20
Stéphanie Simoncini, Anne-Line Chateau, Stéphane Robert, Dilyana Todorova, Catherine Yzydorzick, Romaric Lacroix, Isabelle Ligi, Laurence Louis, Richard Bachelier, Umberto Simeoni, Frédérique Magdinier, Françoise Dignat-George, Florence Sabatier
Senescent cells may exert detrimental effect on microenvironment through the secretion of soluble factors and the release of extracellular vesicles, such as microparticles, key actors in ageing and cardiovascular diseases. We previously reported that sirtuin-1 (SIRT1) deficiency drives accelerated senescence and dysfunction of endothelial colony-forming cells (ECFC) in PT neonates. Because preterm birth (PT) increases the risk for cardiovascular diseases during neonatal period as well as at adulthood, we hypothesized that SIRT1 deficiency could control the biogenesis of microparticles as part of a senescence-associated secretory phenotype (SASP) of PT-ECFC and investigated the related molecular mechanisms...
August 15, 2017: Scientific Reports
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