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Endothelial colony forming cell

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https://www.readbyqxmd.com/read/27899395/functional-definition-of-progenitors-versus-mature-endothelial-cells-reveals-key-soxf-dependent-differentiation-process
#1
Jatin Patel, Elke J Seppanen, Mathieu P Rodero, Ho Yi Wong, Prudence Donovan, Zoltan Neufeld, Nicholas M Fisk, Mathias François, Kiarash Khosrotehrani
BACKGROUND: -During adult life, blood vessel formation is thought to occur via angiogenic processes involving branching from existing vessels. An alternate proposal suggests that neo-vessels form from endothelial progenitors able to assemble the intimal layers. We here aimed to define vessel-resident endothelial progenitors in vivo in a variety of tissues in physiological and pathological situations such as normal aorta, lungs, as well as wound healing, tumors and placenta. METHODS: -Based on protein expression levels of common endothelial markers using flow cytometry, three sub-populations of endothelial cells could be identified among VE-Cadherin+ and CD45- cells...
November 29, 2016: Circulation
https://www.readbyqxmd.com/read/27899144/hypoxia-induced-responses-by-endothelial-colony-forming-cells-are-modulated-by-placental-growth-factor
#2
Michelle B Hookham, Imran H A Ali, Christina L O'Neill, Emer Hackett, Melanie H Lambe, Tina Schmidt, Reinhold J Medina, Sara Chamney, Bharathi Rao, Eibhlin McLoone, David Sweet, Alan W Stitt, Derek P Brazil
BACKGROUND: Endothelial colony-forming cells (ECFCs), also termed late outgrowth endothelial cells, are a well-defined circulating endothelial progenitor cell type with an established role in vascular repair. ECFCs have clear potential for cell therapy to treat ischaemic disease, although the precise mechanism(s) underlying their response to hypoxia remains ill-defined. METHODS: In this study, we isolated ECFCs from umbilical cord blood and cultured them on collagen...
November 29, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27887604/variable-ventilation-improves-pulmonary-function-and-reduces-lung-damage-without-increasing-bacterial-translocation-in-a-rat-model-of-experimental-pneumonia
#3
Raquel F de Magalhães, Cynthia S Samary, Raquel S Santos, Milena V de Oliveira, Nazareth N Rocha, Cintia L Santos, Jamil Kitoko, Carlos A M Silva, Caroline L Hildebrandt, Cassiano F Goncalves-de-Albuquerque, Adriana R Silva, Hugo C Faria-Neto, Vanessa Martins, Vera L Capelozzi, Robert Huhle, Marcelo M Morales, Priscilla Olsen, Paolo Pelosi, Marcelo Gama de Abreu, Patricia R M Rocco, Pedro L Silva
BACKGROUND: Variable ventilation has been shown to improve pulmonary function and reduce lung damage in different models of acute respiratory distress syndrome. Nevertheless, variable ventilation has not been tested during pneumonia. Theoretically, periodic increases in tidal volume (VT) and airway pressures might worsen the impairment of alveolar barrier function usually seen in pneumonia and could increase bacterial translocation into the bloodstream. We investigated the impact of variable ventilation on lung function and histologic damage, as well as markers of lung inflammation, epithelial and endothelial cell damage, and alveolar stress, and bacterial translocation in experimental pneumonia...
November 25, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27881867/endothelial-cell-colony-forming-units-derived-from-malignant-breast-diseases-are-resistant-to-tumor-necrosis-factor-%C3%AE-induced-apoptosis
#4
Chen-Pin Chou, Shih Sheng Jiang, Huay-Ben Pan, Yi-Chen Yen, Hui-Hwa Tseng, Yu-Ting Hung, Ssu-Han Wang, Yu-Lin Chen, Ya-Wen Chen
Mobilisation of endothelial progenitor cells (EPCs) from the bone marrow is a crucial step in the formation of de novo blood vessels, and levels of peripheral blood EPCs have been shown to be elevated in certain malignant states. Using flow cytometry and a Hill-based colony forming unit (CFU) assay, the present study indicated that higher levels of CD34 and vascular endothelial growth factor receptor 2 (VEGFR2) double-positive EPCs, as well as increased formation of endothelial cell colony-forming units (EC-CFUs) are associated with benign and malignant breast diseases, providing possible indicators for breast disease detection...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27879452/umbilical-cord-blood-circulating-progenitor-cells-and-endothelial-colony-forming-cells-are-decreased-in-preeclampsia
#5
Diane L Gumina, Claudine P Black, Vivek Balasubramaniam, Virginia D Winn, Christopher D Baker
Preeclampsia (PE) is a pregnancy-specific disease characterized by the new onset of hypertension and proteinuria. Mothers with PE are known to develop endothelial dysfunction, but its effect on infants has been understudied, as newborns are often asymptomatic. Recent studies indicate that infants born from preeclamptic pregnancies develop endothelial dysfunction including higher blood pressure during childhood and an increased risk of stroke later in life. We hypothesize that PE reduces the number and function of fetal angiogenic progenitor cells and may contribute to this increased risk...
November 21, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27832737/oral-mucosa-harbors-a-high-frequency-of-endothelial-cells-a-novel-postnatal-cell-source-for-angiogenic-regeneration
#6
Jian Zhou, Jason Rogers, Scott Lee, DongMing Sun, Hai Yao, Jeremy J Mao, Kimi Y Kong
Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia and bone fractures but isolation of EPC/ECs from existing cell sources is challenging due to their low endothelial cell frequency. We have isolated EP-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples...
November 10, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27777694/a-reversal-of-age-dependent-proliferative-capacity-of-endothelial-progenitor-cells-from-different-species-origin-in-in-vitro-condition
#7
Mehdi Hassanpour, Omid Cheraghi, Vahid Siavashi, Reza Rahbarghazi, Mohammad Nouri
Introduction: A large number of cardiovascular disorders and abnormalities, notably accelerated vascular deficiencies could be related to aging changes and increased length of life. During the past decades, the discovery of different stem cells facilitates ongoing attempts for attenuating many disorders, especially in vascular beds. Endothelial progenitor cells (EPCs) are a subtype of stem cells that have potent capacity to differentiate into mature endothelial cells (ECs). However, some documented studies reported an age-related decline in proliferation and function of many stem cells...
2016: Journal of Cardiovascular and Thoracic Research
https://www.readbyqxmd.com/read/27761847/triazole-rgd-antagonist-reverts-tgf%C3%AE-1-induced-endothelial-to-mesenchymal-transition-in-endothelial-precursor-cells
#8
Francesca Bianchini, Silvia Peppicelli, Pierangelo Fabbrizzi, Alessio Biagioni, Benedetta Mazzanti, Gloria Menchi, Lido Calorini, Alberto Pupi, Andrea Trabocchi
Fibrosis is the dramatic consequence of a dysregulated reparative process in which activated fibroblasts (myofibroblasts) and Transforming Growth Factor β1 (TGFβ1) play a central role. When exposed to TGFβ1, fibroblast and epithelial cells differentiate in myofibroblasts; in addition, endothelial cells may undergo endothelial-to-mesenchymal transition (EndoMT) and actively participate to the progression of fibrosis. Recently, the role of αv integrins, which recognize the Arg-Gly-Asp (RGD) tripeptide, in the release and signal transduction activation of TGFβ1 became evident...
October 19, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27744579/melanoma-cell-metastasis-via-p-selectin-mediated-activation-of-acid-sphingomyelinase-in-platelets
#9
Katrin Anne Becker, Nadine Beckmann, Constantin Adams, Gabriele Hessler, Melanie Kramer, Erich Gulbins, Alexander Carpinteiro
Metastatic dissemination of cancer cells is one of the hallmarks of malignancy and accounts for approximately 90 % of human cancer deaths. Within the blood vasculature, tumor cells may aggregate with platelets to form clots, adhere to and spread onto endothelial cells, and finally extravasate to form metastatic colonies. We have previously shown that sphingolipids play a central role in the interaction of tumor cells with platelets; this interaction is a prerequisite for hematogenous tumor metastasis in at least some tumor models...
October 15, 2016: Clinical & Experimental Metastasis
https://www.readbyqxmd.com/read/27742844/endothelial-cells-promote-expansion-of-long-term-engrafting-marrow-hematopoietic-stem-and-progenitor-cells-in-primates
#10
Jennifer L Gori, Jason M Butler, Balvir Kunar, Michael G Poulos, Michael Ginsberg, Daniel J Nolan, Zachary K Norgaard, Jennifer E Adair, Shahin Rafii, Hans-Peter Kiem
: : Successful expansion of bone marrow (BM) hematopoietic stem and progenitor cells (HSPCs) would benefit many HSPC transplantation and gene therapy/editing applications. However, current expansion technologies have been limited by a loss of multipotency and self-renewal properties ex vivo. We hypothesized that an ex vivo vascular niche would provide prohematopoietic signals to expand HSPCs while maintaining multipotency and self-renewal. To test this hypothesis, BM autologous CD34(+) cells were expanded in endothelial cell (EC) coculture and transplanted in nonhuman primates...
October 14, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27737702/single-and-double-walled-carbon-nanotubes-enhance-atherosclerogenesis-by-promoting-monocyte-adhesion-to-endothelial-cells-and-endothelial-progenitor-cell-dysfunction
#11
Yuka Suzuki, Saeko Tada-Oikawa, Yasuhiko Hayashi, Kiyora Izuoka, Misa Kataoka, Shunsuke Ichikawa, Wenting Wu, Cai Zong, Gaku Ichihara, Sahoko Ichihara
BACKGROUND: The use of carbon nanotubes has increased lately. However, the cardiovascular effect of exposure to carbon nanotubes remains elusive. The present study investigated the effects of pulmonary exposure to single-walled carbon nanotubes (SWCNTs) and double-walled carbon nanotubes (DWCNTs) on atherosclerogenesis using normal human aortic endothelial cells (HAECs) and apolipoprotein E-deficient (ApoE(-/-)) mice, a model of human atherosclerosis. METHODS: HAECs were cultured and exposed to SWCNTs or DWCNTs for 16 h...
October 13, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27735991/plgf-knockdown-decreases-tumorigenicity-and-stemness-properties-of-spheroid-body-cells-derived-from-gastric-cancer-cells
#12
Fatemeh Mahmoodi, Hassan Akrami
Placental growth factor (PlGF) a member of the vascular endothelial growth factor family regulates some cell processes such as survival, growth of vascular endothelial cells, invasiveness and also involves in pathological angiogenesis and metastasis in most cancers. Cancer stem cells are believed to be the main reason for the tumor relapse and resistance to therapy. These cells have various characteristics as same as normal tissue-specific adult stem cells, including self-renewability and potent to differentiate into various cell types...
October 13, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27709781/activation-of-atp-sensitive-potassium-channels-facilitates-the-function-of-human-endothelial-colony-forming-cells-via-ca-2-akt-enos-pathway
#13
Yan Wu, Meng-Yu He, Jian-Kui Ye, Shu-Ying Ma, Wen Huang, Yong-Yue Wei, Hui Kong, Hong Wang, Xiao-Ning Zeng, Wei-Ping Xie
Accumulating data, including those from our laboratory, have shown that the opening of ATP-sensitive potassium channels (KATP ) plays a protective role in pulmonary vascular diseases (PVD). As maintainers of the endothelial framework, endothelial colony-forming cells (ECFCs) are considered excellent candidates for vascular regeneration in cases of PVD. Although KATP openers (KCOs) have been demonstrated to have beneficial effects on endothelial cells, the impact of KATP on ECFC function remains unclear. Herein, this study investigated whether there is a distribution of KATP in ECFCs and what role KATP play in ECFC modulation...
October 6, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27668588/isolation-of-endothelial-colony-forming-cells-from-blood-samples-collected-from-the-jugular-and-cephalic-veins-of-healthy-adult-horses
#14
Ashley N Sharpe, Wen J Seeto, Randolph L Winter, Qiao Zhong, Elizabeth A Lipke, Anne A Wooldridge
OBJECTIVE To evaluate optimal isolation of endothelial colony-forming cells (ECFCs) from peripheral blood of horses. SAMPLE Jugular and cephalic venous blood samples from 17 adult horses. PROCEDURES Each blood sample was divided; isolation was performed with whole blood adherence (WBA) and density gradient centrifugation (DGC). Isolated cells were characterized by uptake of 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-labeled acetylated low-density lipoprotein (DiI-Ac-LDL), vascular tubule formation, and expression of endothelial (CD34, CD105, vascular endothelial growth factor receptor-2, and von Willebrand factor) and hematopoietic (CD14) cell markers by use of indirect immunofluorescence assay (IFA) and flow cytometry...
October 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27650731/transfer-of-microrna-486-5p-from-human-endothelial-colony-forming-cell-derived-exosomes-reduces-ischemic-kidney-injury
#15
Jose L Viñas, Dylan Burger, Joseph Zimpelmann, Randa Haneef, William Knoll, Pearl Campbell, Alex Gutsol, Anthony Carter, David S Allan, Kevin D Burns
Administration of human cord blood endothelial colony-forming cells (ECFCs) or their exosomes protects mice against kidney ischemia/reperfusion injury. Here we studied the microRNA (miRNA) content of ECFC exosomes and the role of miRNA transfer in kidney and endothelial cell protection. ECFC exosomes were enriched in miR-486-5p, which targets the phosphatase and tensin homolog (PTEN) and the Akt pathway. In cultured endothelial cells exposed to hypoxia, incubation with ECFC exosomes increased miR-486-5p, decreased PTEN, and stimulated Akt phosphorylation...
December 2016: Kidney International
https://www.readbyqxmd.com/read/27649372/the-improvement-of-respiratory-performance-after-phototherapy-induced-epc-mobilization-in-preterm-infants-with-rds
#16
Vahid Siavashi, Simin Asadian, Masoud Taheri-Asl, Homa Babaei, Samaneh Keshavarz, Mohammad Bazaei, Seyed Mahdi Nassiri
Many infants who develop bronchopulmonary dysplasia (BPD) are born with serious respiratory distress syndrome (RDS), which is associated with impaired vascular and alveolar growth. RDS is a breathing disorder that mostly affects preterm infants and occurs in infants whose lungs have not yet been fully developed. The use of surfactant in RDS treatment does not necessarily prevent BPD. Endothelial progenitor cells (EPCs) may contribute to lung angiogenesis for the prevention and treatment of BPD. The aim of this study was to evaluate the therapeutic efficacy of phototherapy for EPC release in preterm infants born with RDS...
September 20, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27639969/existence-of-neural-crest-derived-progenitor-cells-in-normal-and-fuchs-endothelial-dystrophy-corneal-endothelium
#17
Kishore Reddy Katikireddy, Thore Schmedt, Marianne O Price, Francis W Price, Ula V Jurkunas
Human corneal endothelial cells are derived from neural crest and because of postmitotic arrest lack competence to repair cell loss from trauma, aging, and degenerative disorders such as Fuchs endothelial corneal dystrophy (FECD). Herein, we identified a rapidly proliferating subpopulation of cells from the corneal endothelium of adult normal and FECD donors that exhibited features of neural crest-derived progenitor (NCDP) cells by showing absence of senescence with passaging, propensity to form spheres, and increased colony forming efficacy compared with the primary cells...
October 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27638126/impaired-development-and-dysfunction-of-endothelial-progenitor-cells-in-type-2-diabetic-mice
#18
S Tsukada, H Masuda, S Y Jung, J Yun, S Kang, D Y Kim, J H Park, S T Ji, S-M Kwon, T Asahara
AIM: Dysfunction of circulating endothelial progenitor cells (EPCs) has been shown to affect the development of microvascular diseases in diabetes patients. The aim of this study was to elucidate the development and mechanical dysfunction of EPCs in type 2 diabetes (T2D). METHODS: The colony-forming capacity of EPCs and differentiation potential of bone marrow (BM) c-Kit(+)/Sca-I(+) lineage-negative mononuclear cells (KSL) were examined in T2D mice, db/db mice and KKA(y) mice, using EPC colony-forming assay (EPC-CFA)...
September 13, 2016: Diabetes & Metabolism
https://www.readbyqxmd.com/read/27634591/arachidonic-acid-evoked-ca-2-signals-promote-nitric-oxide-release-and-proliferation-in-human-endothelial-colony-forming-cells
#19
Estella Zuccolo, Silvia Dragoni, Valentina Poletto, Paolo Catarsi, Daniele Guido, Alessandra Rappa, Marta Reforgiato, Francesco Lodola, Dmitry Lim, Vittorio Rosti, Germano Guerra, Francesco Moccia
Arachidonic acid (AA) stimulates endothelial cell (EC) proliferation through an increase in intracellular Ca(2+) concentration ([Ca(2+)]i), that, in turn, promotes nitric oxide (NO) release. AA-evoked Ca(2+) signals are mainly mediated by Transient Receptor Potential Vanilloid 4 (TRPV4) channels. Circulating endothelial colony forming cells (ECFCs) represent the only established precursors of ECs. In the present study, we therefore, sought to elucidate whether AA promotes human ECFC (hECFC) proliferation through an increase in [Ca(2+)]i and the following activation of the endothelial NO synthase (eNOS)...
September 12, 2016: Vascular Pharmacology
https://www.readbyqxmd.com/read/27632536/il-1-coordinates-the-neutrophil-response-to-c-albicans-in-the-oral-mucosa
#20
Simon Altmeier, Albulena Toska, Florian Sparber, Alvaro Teijeira, Cornelia Halin, Salomé LeibundGut-Landmann
Mucosal infections with Candida albicans belong to the most frequent forms of fungal diseases. Host protection is conferred by cellular immunity; however, the induction of antifungal immunity is not well understood. Using a mouse model of oropharyngeal candidiasis (OPC) we show that interleukin-1 receptor (IL-1R) signaling is critical for fungal control at the onset of infection through its impact on neutrophils at two levels. We demonstrate that both the recruitment of circulating neutrophils to the site of infection and the mobilization of newly generated neutrophils from the bone marrow depended on IL-1R...
September 2016: PLoS Pathogens
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