keyword
MENU ▼
Read by QxMD icon Read
search

Translational AND imaging

keyword
https://www.readbyqxmd.com/read/29785487/rapid-monitoring-of-the-target-protein-expression-with-a-fluorescent-signal-based-on-a-dicistronic-construct-in-escherichia-coli
#1
Chang-Ye Hui, Yan Guo, Wen Zhang, Xian-Qing Huang
Real-time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time-consuming and labor-intensive procedures, such as polyacrylamide gel electrophoresis, immunoblot analysis, and biological activity assays. We describe a simple, fast, and high sensitive assay for detecting heterologous proteins production in bacteria either at the overall level (fluorescence spectrophotometry) or at the individual level (fluorescence microscopic image) in this study...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29782671/enhanced-performance-of-a-molecular-photoacoustic-imaging-agent-by-encapsulation-in-mesoporous-silicon-nanoparticles
#2
Jinyoung Kang, Dokyoung Kim, Junxin Wang, Yunho Han, Jonathan M Zuidema, Ali Hariri, Ji-Ho Park, Jesse V Jokerst, Michael J Sailor
Photoacoustic (PA) imaging allows visualization of the physiology and pathology of tissues with good spatial resolution and relatively deep tissue penetration. The method converts near-infrared (NIR) laser excitation into thermal expansion, generating pressure transients that are detected with an acoustic transducer. Here, we find that the response of the PA contrast agent indocyanine green (ICG) can be enhanced 17-fold when it is sealed within a rigid nanoparticle. ICG encapsulated in particles composed of porous silicon (pSiNP), porous silica, or calcium silicate all show greater PA contrast relative to equivalent quantities of free ICG, with the pSiNPs showing the strongest enhancement...
May 21, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29782657/phantom-clinical-and-texture-indices-evaluation-and-optimization-of-a-penalized-likelihood-image-reconstruction-method-q-clear-on-a-bgo-pet-ct-scanner
#3
Gabriel Reynés-Llompart, Cristina Gámez-Cenzano, José Luís Vercher-Conejero, Aida Sabaté-Llobera, Nahúm Calvo-Malvar, Josep M Martí-Climent
INTRODUCTION: The aim of this study was to evaluate the behavior of a penalized-likelihood image reconstruction method (Q.Clear) under different count statistics and lesion-to-background ratios (LBR) on a BGO scanner, in order to obtain an optimum penalization factor (β value) to study and optimize for different acquisition protocols and clinical goals. METHODS: Both phantom and patient images were evaluated. Data from an image quality phantom were acquired using different Lesion-to-Background ratios and acquisition times...
May 21, 2018: Medical Physics
https://www.readbyqxmd.com/read/29781658/rapid-instruction-based-task-learning-ritl-in-schizophrenia
#4
Julia M Sheffield, Hannes Ruge, Sridhar Kandala, Deanna M Barch
Individuals with schizophrenia demonstrate broad impairments in neurocognitive functioning as measured through laboratory-based tasks. Neuropsychological measures depend on rapid instruction-based task learning (RITL), the ability to rapidly translate task instruction into goal-directed behavior. Here, the authors present the first known investigation of RITL in schizophrenia and aim to test whether RITL deficits exist in schizophrenia, are associated with abnormal brain activation, and contribute to the generalized cognitive deficit...
May 21, 2018: Journal of Abnormal Psychology
https://www.readbyqxmd.com/read/29781102/hierarchical-deep-convolutional-neural-networks-combine-spectral-and-spatial-information-for-highly-accurate-raman-microscopy-based-cytopathology
#5
Sascha D Krauß, Raphael Roy, Hesham K Yosef, Tatjana Lechtonen, Samir F El-Mashtoly, Klaus Gerwert, Axel Mosig
Hierarchical variants of so-called deep convolutional neural networks (DCNNs) have facilitated breakthrough results for numerous pattern recognition tasks in recent years. We assess the potential of these novel whole-image classifiers for Raman microscopy based cytopathology. Conceptually, DCNNs facilitate a flexible combination of spectral and spatial information for classifying cellular images as healthy or cancer affected cells. As we demonstrate, this conceptual advantage translates into practice, where DCNNs exceed the accuracy of both conventional classifiers based on pixel spectra as well as classifiers based on morphological features extracted from Raman microscopic images...
May 21, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29780718/robotic-pancreas-drainage-procedure-for-chronic-pancreatitis-robotic-lateral-pancreaticojejunostomy-puestow-procedure
#6
Adeel S Khan, Imran Siddiqui, Dionisios Vrochides, John B Martinie
Lateral pancreaticojejunostomy (LPJ), also known as the Puestow procedure, is a complex surgical procedure reserved for patients with refractory chronic pancreatitis (CP) and a dilated pancreatic duct. Traditionally, this operation is performed through an open incision, however, recent advancements in minimally invasive techniques have made it possible to perform the surgery using laparoscopic and robotic techniques with comparable safety. Though we do not have enough data yet to prove superiority of one over the other, the robotic approach appears to have an advantage over the laparoscopic technique in better visualization through 3-dimensional (3D) imaging and availability of wristed instruments for more precise actions, which may translate into superior outcomes...
2018: Journal of Visualized Surgery
https://www.readbyqxmd.com/read/29780511/through-tissue-imaging-of-a-live-breast-cancer-tumour-model-using-handheld-surface-enhanced-spatially-offset-resonance-raman-spectroscopy-sesorrs
#7
Fay Nicolson, Lauren E Jamieson, Samuel Mabbott, Konstantinos Plakas, Neil C Shand, Michael R Detty, Duncan Graham, Karen Faulds
In order to improve patient survival and reduce the amount of unnecessary and traumatic biopsies, non-invasive detection of cancerous tumours is of imperative and urgent need. Multicellular tumour spheroids (MTS) can be used as an ex vivo cancer tumour model, to model in vivo nanoparticle (NP) uptake by the enhanced permeability and retention (EPR) effect. Surface enhanced spatially offset Raman spectroscopy (SESORS) combines both surface enhanced Raman spectroscopy (SERS) and spatially offset Raman spectroscopy (SORS) to yield enhanced Raman signals at much greater sub-surface levels...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29779140/knee-motion-symmetry-was-not-restored-in-patients-with-unilateral-bi-cruciate-retaining-total-knee-arthroplasty-in-vivo-three-dimensional-kinematic-analysis
#8
Paul Arauz, Yun Peng, Young-Min Kwon
PURPOSE: Although unilateral total knee arthroplasty (TKA) is successful in restoring function, there are concerns in relation to asymmetric gait patterns. Bi-cruciate retaining (BCR) TKA design preserves both anterior and posterior cruciate ligaments with the potential to restore normal joint kinematics. The purpose of this study was to investigate the in vivo three-dimensional (3D) kinematics of the knee in unilateral BCR TKA patients during gait. METHODS: Twenty-nine unilateral BCR TKA patients were evaluated for both knees during treadmill gait using a combined computer tomography and dual fluoroscopic imaging system approach...
May 19, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29776382/sternal-instability-measured-with-radiostereometric-analysis-a-study-of-method-feasibility-accuracy-and-precision
#9
Rikke Falsig Vestergaard, Kjeld Søballe, John Michael Hasenkam, Maiken Stilling
BACKGROUND: A small, but unstable, saw-gap may hinder bone-bridging and induce development of painful sternal dehiscence. We propose the use of Radiostereometric Analysis (RSA) for evaluation of sternal instability and present a method validation. METHODS: Four bone analogs (phantoms) were sternotomized and tantalum beads were inserted in each half. The models were reunited with wire cerclage and placed in a radiolucent separation device. Stereoradiographs (n = 48) of the phantoms in 3 positions were recorded at 4 imposed separation points...
May 18, 2018: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/29776058/phase-transitions-in-a-system-of-hard-y-shaped-particles-on-the-triangular-lattice
#10
Dipanjan Mandal, Trisha Nath, R Rajesh
We study the different phases and the phase transitions in a system of Y-shaped particles, examples of which include immunoglobulin-G and trinaphthylene molecules, on a triangular lattice interacting exclusively through excluded volume interactions. Each particle consists of a central site and three of its six nearest neighbors chosen alternately, such that there are two types of particles which are mirror images of each other. We study the equilibrium properties of the system using grand canonical Monte Carlo simulations that implement an algorithm with cluster moves that is able to equilibrate the system at densities close to full packing...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29774594/in-vivo-characterization-of-3d-skull-and-brain-motion-during-dynamic-head-vibration-using-magnetic-resonance-elastography
#11
Ziying Yin, Yi Sui, Joshua D Trzasko, Phillip J Rossman, Armando Manduca, Richard L Ehman, John Huston
PURPOSE: To introduce newly developed MR elastography (MRE)-based dual-saturation imaging and dual-sensitivity motion encoding schemes to directly measure in vivo skull-brain motion, and to study the skull-brain coupling in volunteers with these approaches. METHODS: Six volunteers were scanned with a high-performance compact 3T-MRI scanner. The skull-brain MRE images were obtained with a dual-saturation imaging where the skull and brain motion were acquired with fat- and water-suppression scans, respectively...
May 17, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29774062/-in-vivo-imaging-biomarkers-of-neuroinflammation-in-the-development-and-assessment-of-stroke-therapies-towards-clinical-translation
#12
REVIEW
Bastian Zinnhardt, Maximilian Wiesmann, Lisa Honold, Cristina Barca, Michael Schäfers, Amanda J Kiliaan, Andreas H Jacobs
Modulation of the inflammatory microenvironment after stroke opens a new avenue for the development of novel neurorestorative therapies in stroke. Understanding the spatio-temporal profile of (neuro-)inflammatory imaging biomarkers in detail thereby represents a crucial factor in the development and application of immunomodulatory therapies. The early integration of quantitative molecular imaging biomarkers in stroke drug development may provide key information about (i) early diagnosis and follow-up, (ii) spatio-temporal drug-target engagement (pharmacodynamic biomarker), (iii) differentiation of responders and non-responders in the patient cohort (inclusion/exclusion criteria; predictive biomarkers), and (iv) the mechanism of action...
2018: Theranostics
https://www.readbyqxmd.com/read/29773299/diffusion-mri-in-acute-nervous-system-injury
#13
Matthew D Budde, Nathan P Skinner
Diffusion weighted magnetic resonance imaging (DWI) and related techniques such as diffusion tensor imaging (DTI) are uniquely sensitive to the microstructure of the brain and spinal cord. In the acute aftermath of nervous system injury, for example, DWI reveals changes caused by injury that remains invisible on other MRI contrasts such as T2 -weighted imaging. This ability has led to a demonstrated clinical utility in cerebral ischemia. However, despite strong promise in preclinical models and research settings, DWI has not been as readily adopted for other acute injuries such as traumatic spinal cord, brain, or peripheral nerve injury...
April 30, 2018: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/29772690/peritoneal-carcinomatosis-targeting-with-tumor-homing-peptides
#14
REVIEW
Lorena Simón-Gracia, Hedi Hunt, Tambet Teesalu
Over recent decades multiple therapeutic approaches have been explored for improved management of peritoneally disseminated malignancies-a grim condition known as peritoneal carcinomatosis (PC). Intraperitoneal (IP) administration can be used to achieve elevated local concentration and extended half-life of the drugs in the peritoneal cavity to improve their anticancer efficacy. However, IP-administered chemotherapeutics have a short residence time in the IP space, and are not tumor selective. An increasing body of work suggests that functionalization of drugs and nanoparticles with targeting peptides increases their peritoneal retention and provides a robust and specific tumor binding and penetration that translates into improved therapeutic response...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29771683/an-individualized-strategy-to-estimate-the-effect-of-deformable-registration-uncertainty-on-accumulated-dose-in-the-upper-abdomen
#15
Yibing Wang, Steven F Petit, Eliana Vasquez Osorio, Vikas Gupta, Alejandra Méndez Romero, Ben J M Heijmen
In the abdomen, it is challenging to assess the accuracy of deformable image registration (DIR) for individual patients, due to the lack of clear anatomical landmarks, which can hamper clinical applications that require high accuracy DIR, such as adaptive radiotherapy. In this study, we propose and evaluate a methodology for estimating the impact of uncertainties in DIR on calculated accumulated dose in the upper abdomen, in order to aid decision making in adaptive treatment approaches. 
 Sixteen liver metastasis patients treated with SBRT were evaluated...
May 17, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29771238/on-the-direct-acquisition-of-beam-s-eye-view-images-in-mri-for-integration-with-external-beam-radiotherapy
#16
Keith Wachowicz, Brad Murray, B Gino Fallone
The recent interest in the integration of external beam radiotherapy with a magnetic resonance (MR) imaging unit offers the potential for real-time adaptive tumour tracking during radiation treatment. The tracking of large tumours which follow a rapid trajectory may best be served by the generation of a projection image from the perspective of the beam source, or "beam's eye view" (BEV). This type of image projection represents the path of the radiation beam, thus enabling rapid compensations for target translations, rotations and deformations, as well time-dependent critical structure avoidance...
May 17, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29770596/self-referenced-axial-chromatic-dispersion-measurement-in-multiphoton-microscopy-through-2-color-thg-imaging
#17
Yu Du, Ziwei Zhuang, Jiexing He, Hongji Liu, Ping Qiu, Ke Wang
With tunable excitation light, multiphoton microscopy (MPM) is widely used for imaging biological structures at subcellular resolution. Axial chromatic dispersion, present in virtually every transmissive optical system including the multiphoton microscope, leads to focal (and the resultant image) plane separation. Here we demonstrate experimentally a technique to measure the axial chromatic dispersion in a multiphoton microscope, using simultaneous 2-color third-harmonic generation (THG) imaging excited by a 2-color soliton source with tunable wavelength separation...
May 16, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29770481/effect-of-head-motion-on-mri-b-0-field-distribution
#18
Jiaen Liu, Jacco A de Zwart, Peter van Gelderen, Joseph Murphy-Boesch, Jeff H Duyn
PURPOSE: To identify and characterize the sources of B0 field changes due to head motion, to reduce motion sensitivity in human brain MRI. METHODS: B0 fields were measured in 5 healthy human volunteers at various head poses. After measurement of the total field, the field originating from the subject was calculated by subtracting the external field generated by the magnet and shims. A subject-specific susceptibility model was created to quantify the contribution of the head and torso...
May 16, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29769696/quantum-cascade-laser-based-infrared-microscopy-for-label-free-and-automated-cancer-classification-in-tissue-sections
#19
Claus Kuepper, Angela Kallenbach-Thieltges, Hendrik Juette, Andrea Tannapfel, Frederik Großerueschkamp, Klaus Gerwert
A feasibility study using a quantum cascade laser-based infrared microscope for the rapid and label-free classification of colorectal cancer tissues is presented. Infrared imaging is a reliable, robust, automated, and operator-independent tissue classification method that has been used for differential classification of tissue thin sections identifying tumorous regions. However, long acquisition time by the so far used FT-IR-based microscopes hampered the clinical translation of this technique. Here, the used quantum cascade laser-based microscope provides now infrared images for precise tissue classification within few minutes...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769198/mathematical-modeling-predicts-response-to-chemotherapy-and-drug-combinations-in-ovarian-cancer
#20
Emilia Kozłowska, Anniina Färkkilä, Tuulia Vallius, Olli Carpén, Jukka Kemppainen, Seija Grénman, Rainer Lehtonen, Johanna Hynninen, Sakari Hietanen, Sampsa Hautaniemi
Platinum-based chemotherapy constitutes the backbone of clinical care in advanced solid cancers such as high-grade serous ovarian cancer (HGSOC) and has prolonged survival of millions of cancer patients. Most of these patients, however, become resistant to chemotherapy, which generally leads to a fatal refractory disease. We present a comprehensive stochastic mathematical model and simulator approach to describe platinum resistance and standard-of-care (SOC) therapy in HGSOC. We used pre- and post-treatment clinical data, including 18F-FDG-PET/CT images, to reliably estimate the model parameters and simulate "virtual HGSOC patients...
May 16, 2018: Cancer Research
keyword
keyword
64054
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"