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https://www.readbyqxmd.com/read/28092137/isolation-and-characterization-of-rhizobium-sp-strain-ys-1r-that-degrades-lignin-in-plant-biomass
#1
Colin A Jackson, Matthew B Couger, Madhu Prabhakaran, Karthikeyan D Ramachandriya, Patricia Canaan, Babu Z Fathepure
AIMS: The aim of this work was to isolate novel lignin degrading organisms. METHODS AND RESULTS: Several pure cultures of bacteria that degrade lignin were isolated from bacterial consortia developed from decaying biomass. Among the isolates, Rhizobium sp. strain YS-1r (closest relative of Rhizobium petrolearium Strain SL-1) was explored for its lignin degrading ability. Microcosm studies showed that strain YS-1r was able to degrade a variety of lignin monomers, dimers, and also native lignin in switchgrass and alfalfa...
January 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28090344/tasting-profile-affects-adoption-of-caloric-beverage-reduction-in-a-randomized-weight-loss-intervention
#2
G Turner-McGrievy, X Wang, B Popkin, D F Tate
OBJECTIVE: The aim of this study was to examine differences in rates of non-caloric beverage adoption by participants classified as sweet likers (SLs) or sweet dislikers (measured using a behavioural tasting task). METHODS: Data are a sub-study from a 6-month, three-group, randomized weight loss trial (CHOICE) (body mass index 36.3 ± 5.8 kg m(-2), 84% female, aged 42.2 ± 10.9 years, 53% African-American) comparing the replacement of caloric beverages with either non-caloric sweetened beverages (diet) or water (water) compared with a control group...
December 2016: Obesity Science & Practice
https://www.readbyqxmd.com/read/28088470/the-effects-of-dietary-kefir-and-low-molecular-weight-sodium-alginate-on-serum-immune-parameters-resistance-against-streptococcus-agalactiae-and-growth-performance-in-nile-tilapia-oreochromis-niloticus
#3
Hien Van Doan, Seyed Hossein Hoseinifar, Wanaporn Tapingkae, Pimporn Khamtavee
The present study evaluates the effects of dietary kefir and low molecular weight sodium alginate (LWMSA) (singular or combined) on non-specific immune response, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Fish with average weight of 18.60 ± 0.04 g were supplied and randomly stocked in sixteen glass tanks (150 L) at density of 20 fish per tank. Fish were fed experimental diets as follows: 0 g kg(-1) LMWSA (Control, Diet 1), 10 g kg(-1) LMWSA (Diet 2), 40 g kg(-1) kefir (Diet 3), and 10 g kg(-1) LMWSA + 40 g kg(-1) kefir (Diet 4) for 50 days...
January 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28087713/functional-genomics-reveals-that-tumors-with-activating-phosphoinositide-3-kinase-mutations-are-dependent-on-accelerated-protein-turnover
#4
Teresa Davoli, Kristen E Mengwasser, Jingjing Duan, Ting Chen, Camilla Christensen, Eric C Wooten, Anthony N Anselmo, Mamie Z Li, Kwok-Kin Wong, Kristopher T Kahle, Stephen J Elledge
Activating mutations in the phosphoinositide 3-kinase (PI3K) signaling pathway are frequently identified in cancer. To identify pathways that support PI3K oncogenesis, we performed a genome-wide RNAi screen in isogenic cell lines harboring wild-type or mutant PIK3CA to search for PI3K synthetic-lethal (SL) genes. A combined analysis of these results with a meta-analysis of two other large-scale RNAi screening data sets in PI3K mutant cancer cell lines converged on ribosomal protein translation and proteasomal protein degradation as critical nononcogene dependencies for PI3K-driven tumors...
December 15, 2016: Genes & Development
https://www.readbyqxmd.com/read/28081139/long-latency-somatosensory-evoked-potentials-of-the-subthalamic-nucleus-in-patients-with-parkinson-s-disease
#5
Carlos Trenado, Saskia Elben, Lena Friggemann, Sonja Gruhn, Stefan Jun Groiss, Jan Vesper, Alfons Schnitzler, Lars Wojtecki
Somatosensory evoked potentials (SSEPs) are a viable way to measure processing of somatosensory information. SSEPs have been described at the scalp and the cortical level by electroencephalographic, magnetoencephalographic and intracranial cortical recordings focusing on short-latency (SL; latency<40 ms) and long-latency (LL; latency>40 ms) SSEPs as well as by deep brain stimulation (DBS) electrode studies targeting SL-SSEPs. Unfortunately, LL-SSEPs have not been addressed at the subcortical level aside from the fact that studies targeting the characteristics and generators of SSEPs have been neglected for the last ten years...
2017: PloS One
https://www.readbyqxmd.com/read/28073072/life-cycle-of-blastocrithidia-papi-sp-n-kinetoplastea-trypanosomatidae-in-pyrrhocoris-apterus-hemiptera-pyrrhocoridae
#6
Alexander O Frolov, Marina N Malysheva, Anna I Ganyukova, Vyacheslav Yurchenko, Alexei Y Kostygov
Blastocrithidia papi sp. n. is a cyst-forming trypanosomatid parasitizing firebugs (Pyrrhocoris apterus). It is a member of the Blastocrithidia clade and a very close relative of B. largi, to which it is almost identical through its SSU rRNA gene sequence. However, considering the SL RNA gene these two species represent quite distinct, not even related typing units. Morphological analysis of the new species revealed peculiar or even unique features, which may be useful for future taxonomic revision of the genus Blastocrithidia...
November 16, 2016: European Journal of Protistology
https://www.readbyqxmd.com/read/28069242/comparison-of-sonochemiluminescence-images-using-image-analysis-techniques-and-identification-of-acoustic-pressure-fields-via-simulation
#7
T Joyce Tiong, Tissa Chandesa, Yeow Hong Yap
One common method to determine the existence of cavitational activity in power ultrasonics systems is by capturing images of sonoluminescence (SL) or sonochemiluminescence (SCL) in a dark environment. Conventionally, the light emitted from SL or SCL was detected based on the number of photons. Though this method is effective, it could not identify the sonochemical zones of an ultrasonic systems. SL/SCL images, on the other hand, enable identification of 'active' sonochemical zones. However, these images often provide just qualitative data as the harvesting of light intensity data from the images is tedious and require high resolution images...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069020/work-outcome-in-persons-with-musculoskeletal-diseases-comparison-with-other-chronic-diseases-the-role-of-musculoskeletal-diseases-in-multimorbidity
#8
Antje van der Zee-Neuen, Polina Putrik, Sofia Ramiro, Andras Keszei, Rob de Bie, Astrid Chorus, Annelies Boonen
BACKGROUND: Chronic diseases and multimorbidity are increasingly common among persons in working age. This study explores the impact of type, number and combinations of chronic diseases with focus on the role of MSKD on (1) adverse work status (i.e. work disability (WD), economic unemployment (UE) or receiving a living allowance (LA)) and on (2) the occurrence of sick leave. METHODS: Subjects participating in a Dutch household survey, who were ≤65 years and could have paid work, provided data on socio-demographics and nine physician diagnosed chronic diseases...
January 10, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28061463/prevalence-of-psychological-symptoms-among-ebola-survivors-and-healthcare-workers-during-the-2014-2015-ebola-outbreak-in-sierra-leone-a-cross-sectional-study
#9
Dong Ji, Ying-Jie Ji, Xue-Zhang Duan, Wen-Gang Li, Zhi-Qiang Sun, Xue-Ai Song, Yu-Hua Meng, Hong-Mei Tang, Fang Chu, Xiao-Xia Niu, Guo-Feng Chen, Jin Li, Hui-Juan Duan
The 2014-2015 Ebola epidemic was considered to be the largest and most complex outbreak, which caused 11,310 reported deaths. The epidemic disease can cause a mental health crisis, however, there is only a small amount of scientific literature available related to this health issue so far. We evaluated the psychological symptoms of 161 participants including Ebola survivors and healthcare workers in Sierra Leone, analyzed the impact of job classification, education level on psychological status. We found that the order of total general severity index (GSI) scores from high to low was EVD survivors, SL medical staff, SL logistic staff, SL medical students, and Chinese medical staff...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28060274/gap-junctional-intercellular-communication-a-functional-biomarker-to-assess-adverse-effects-of-toxicants-and-toxins-and-health-benefits-of-natural-products
#10
Brad L Upham, Iva Sovadinová, Pavel Babica
This protocol describes a scalpel loading-fluorescent dye transfer (SL-DT) technique that measures intercellular communication through gap junction channels, which is a major intercellular process by which tissue homeostasis is maintained. Interruption of gap junctional intercellular communication (GJIC) by toxicants, toxins, drugs, etc. has been linked to numerous adverse health effects. Many genetic-based human diseases have been linked to mutations in gap junction genes. The SL-DT technique is a simple functional assay for the simultaneous assessment of GJIC in a large population of cells...
December 25, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28059683/spinopelvic-sagittal-imbalance-as-a-risk-factor-for-adjacent-segment-disease-after-single-segment-posterior-lumbar-interbody-fusion
#11
Tomiya Matsumoto, Shinya Okuda, Takafumi Maeno, Tomoya Yamashita, Ryoji Yamasaki, Tsuyoshi Sugiura, Motoki Iwasaki
OBJECTIVE The importance of spinopelvic balance and its implications for clinical outcomes after spinal arthrodesis has been reported in recent studies. However, little is known about the relationship between adjacent-segment disease (ASD) after lumbar arthrodesis and spinopelvic alignment. The purpose of this study was to clarify the relationship between spinopelvic radiographic parameters and symptomatic ASD after L4-5 single-level posterior lumbar interbody fusion (PLIF). METHODS This was a retrospective 1:5 matched case-control study...
January 6, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/28052758/prognostic-values-of-eortc-qlq-c30-and-qlq-hcc18-index-scores-in-patients-with-hepatocellular-carcinoma-clinical-application-of-health-related-quality-of-life-data
#12
Leung Li, Frankie Kf Mo, Stephen L Chan, Edwin P Hui, Nelson Sl Tang, Jane Koh, Linda Ks Leung, Annette Ny Poon, Joyce Hui, Cheuk M Chu, Kit F Lee, Brigette By Ma, Paul Bs Lai, Anthony Tc Chan, Simon Ch Yu, Winnie Yeo
BACKGROUND: Health-related quality-of-life (HRQOL) assessment with EORTC QLQ-C30 was prognostic for overall survival (OS) in patients with advance-stage hepatocellular carcinoma (HCC), but no data existed for early-stage patients. The HCC-specific QLQ-HCC18 has not been evaluated for prognostic value in HCC patients. Utilization of raw HRQOL data in clinical setting has been impractical and non-meaningful. Therefore we developed index scores of QLQ-C30 and QLQ-HCC18 in an attempt to enable clinical utilization of these HRQOL measurements...
January 4, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28049451/feasibility-study-of-hiv-sentinel-surveillance-using-pmtct-data-in-cameroon-from-scientific-success-to-programmatic-failure
#13
Serge C Billong, Jacob Dee, Joseph Fokam, Georges Nguefack-Tsague, Gabriel L Ekali, Raoul Fodjo, Edith S Temgoua, Edson-Joan Billong, Samuel M Sosso, Jembia J Mosoko, Francisca Monebenimp, Alexis Ndjolo, Anne-Cecile Z-K Bissek, Omotayo Bolu, Jean-Bosco N Elat
BACKGROUND: In low-income countries (LICs), HIV sentinel surveillance surveys (HIV-SSS) are recommended in between two demographic and health surveys, due to low-cost than the latter. Using the classical unlinked anonymous testing (UAT), HIV-SSS among pregnant women raised certain ethical and financial challenges. We therefore aimed at evaluating how to use prevention of mother-to-child transmission of HIV (PMTCT) routine data as an alternative approach for HIV-SSS in LICs. METHODS: A survey conducted through 2012 among first antenatal-care attendees (ANC1) in the ten regions of Cameroon...
January 3, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28047393/su-f-r-28-correction-of-fch-pet-bladder-uptake-using-virtual-sinograms-and-investigation-of-its-impact-on-the-quantification-of-prostate-textural-characteristics
#14
S Laberge, J Beauregard, L Archambault
PURPOSE: Textural biomarkers as a tool for quantifying intratumoral heterogeneity hold great promise for diagnosis and early assessment of treatment response in prostate cancer. However, spill-in counts from the bladder uptake are suspected to have an impact on the textural measurements of the prostate volume. This work proposes a correction method for the FCh-PET bladder uptake and investigates its impact on intraprostatic textural properties. METHODS: Two patients with PC received pre-treatment dynamic FCh-PET scans reconstructed at four time points (interval: 2 min), for which prostate and bladder contours were obtained...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28045338/systolic-mitral-annulus-velocity-is-a-sensitive-index-for-changes-in-left-ventricular-systolic-function-during-inotropic-therapy-in-patients-with-acute-heart-failure
#15
Trygve Husebye, Jan Eritsland, Reidar Bjørnerheim, Geir Ø Andersen
BACKGROUND: Echocardiography is recommended for assessment of left ventricular systolic function in patients with acute heart failure but few randomised trials have validated techniques like tissue Doppler (TDI) and speckle tracking (STE) in patients with acute heart failure following ST-elevation myocardial infarction. METHODS: This was a substudy from the LEAF (LEvosimendan in Acute heart Failure following myocardial infarction) trial (NCT00324766 ), which randomised 61 patients developing acute heart failure, including cardiogenic shock, within 48 hours after ST-elevation myocardial infarction, double-blind to a 25-hour infusion of levosimendan or placebo...
January 1, 2017: European Heart Journal. Acute Cardiovascular Care
https://www.readbyqxmd.com/read/28039392/molecular-characterization-of-tomato-%C3%AE-1-3-4-fucosidase-a-member-of-glycosyl-hydrolase-family-29-involved-in-the-degradation-of-plant-complex-type-n-glycans
#16
Md Ziaur Rahman, Megumi Maeda, Satsuki Itano, Anowar Hossain, Takeshi Ishimizu, Yoshinobu Kimura
In this study, we identified a gene in tomato that encodes an acidic α-fucosidase (LOC101254568 or Solyc03g006980, α-Fuc'ase S1-1), which may be involved in the turnover of plant complex-type N-glycans. Recombinant α-Fuc'ase S1-1 (rFuc'ase S1-1) was expressed using a baculovirus-insect cell expression system. rFuc'ase Sl-1 is 55 kDa in size and has an optimum pH around 4.5. It substantially hydrolyzed the non-reducing terminal α1,3-fucose residue on LNFP III and α1,4-fucose residues of Le(a) epitopes on plant complex-type N-glycans, but not the α1,2-fucose residue on LNFP I or the α1,3-fucose residue on pyridylaminated Fucα1-3GlcNAc...
December 30, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/28035290/pathological-location-of-cranial-nerves-in-petroclival-lesions-how-to-avoid-their-injury-during-anterior-petrosal-approach
#17
Hamid Borghei-Razavi, Ryosuke Tomio, Seyed-Mohammad Fereshtehnejad, Shunsuke Shibao, Uta Schick, Masahiro Toda, Kazunari Yoshida, Takeshi Kawase
Objectives Numerous surgical approaches have been developed to access the petroclival region. The Kawase approach, through the middle fossa, is a well-described option for addressing cranial base lesions of the petroclival region. Our aim was to gather data about the variation of cranial nerve locations in diverse petroclival pathologies and clarify the most common pathologic variations confirmed during the anterior petrosal approach. Method A retrospective analysis was made of both videos and operative and histologic records of 40 petroclival tumors from January 2009 to September 2013 in which the Kawase approach was used...
February 2016: Journal of Neurological Surgery. Part B, Skull Base
https://www.readbyqxmd.com/read/28032435/effects-of-rest-intervals-and-training-loads-on-metabolic-stress-and-muscle-hypertrophy
#18
Julius Fink, Naoki Kikuchi, Koichi Nakazato
We investigated the effects of volume-matched resistance training (RT) with different training loads and rest intervals on acute responses and long-term muscle and strength gains. Ten subjects trained with short rest (30 s) combined with low load (20 RM) (SL) and ten subjects performed the same protocol with long rest (3 min) and high load (8 RM) (LH). Cross-sectional area (CSA) of the upper arm was measured by magnetic resonance imaging before and after 8 weeks of training. Acute stress markers such as growth hormone (GH) and muscle thickness (MT) changes have been assessed pre and post a single RT session...
December 28, 2016: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/28030494/traumatic-colon-injury-in-damage-control-laparotomy-a-multicenter-trial-is-it-safe-to-do-a-delayed-anastomosis
#19
Leah Tatebe, Andrew Jennings, Ken Tatebe, Alexandra Handy, Purvi Prajapati, Michael Smith, Tai Do, Gerald O Ogola, Rajesh R Gandhi, Therese M Duane, Stephen Luk, Laura B Petrey
BACKGROUND: Delayed colonic anastomosis after damage control laparotomy (DCL) is an alternative to colostomies during a single laparotomy (SL) in high-risk patients. However, literature suggests increased colonic leak rates up to 27% with DCL, and various reported risk factors. We evaluated our regional experience to determine if delayed colonic anastomosis was associated with worse outcomes. METHODS: A multi-center retrospective cohort study was performed across three Level I Trauma Centers encompassing traumatic colon injuries from January 2006 through June 2014...
December 23, 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28028799/residual-force-enhancement-is-regulated-by-titin-in-skeletal-and-cardiac-myofibrils
#20
Nabil Shalabi, Anabelle Cornachione, Felipe Leite, Srikar Vengallatore, Dilson E Rassier
When a skeletal muscle contracts isometrically, the muscle produces a force relative to the final isometric sarcomere length (SL). However, when the same final SL is reached by stretching the muscle while it contracts, the muscle produces a relatively higher force: a phenomenon commonly referred to as residual force enhancement. In this study, we investigated residual force enhancement in rabbit skeletal psoas myofibrils and, for the first time, cardiac papillary myofibrils. A custom-built atomic force microscope was used in experiments that stretched myofibrils before and after inhibiting myosin and actin interactions to determine whether the different cardiac and skeletal titin isoforms regulate residual force enhancement...
December 27, 2016: Journal of Physiology
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