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https://www.readbyqxmd.com/read/29143996/long-non-coding-rna-h19-suppresses-retinoblastoma-progression-via-counteracting-mir-17-92-cluster
#1
Aihui Zhang, Weiwei Shang, Qiaoli Nie, Ting Li, Suhui Li
Long non-coding RNAs (lncRNAs) are frequently dysregulated and play important roles in many cancers. lncRNA H19 is one of the earliest discovered lncRNAs which has diverse roles in different cancers. However, the expression, roles, and action mechanisms of H19 in retinoblastoma are still largely unknown. In this study, we found that H19 is downregulated in retinoblastoma tissues and cell lines. Gain-of-function and loss-of-function assays showed that H19 inhibits retinoblastoma cell proliferation, induces retinoblastoma cell cycle arrest and cell apoptosis...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29138818/%C3%AE-%C3%A2-ecdysterone-promotes-autophagy-and-inhibits-apoptosis-in-osteoporotic-rats
#2
Yang-Hua Tang, Zhen-Shuang Yue, Da-Wei Xin, Lin-Ru Zeng, Zhen-Fei Xiong, Zhong-Qing Hu, Can-Da Xu
Osteoporosis is an aging process of skeletal tissues with characteristics of reductions in bone mass and microarchitectural deterioration of bone tissue. The present study aimed to investigate the effects of glucocorticoid‑induced osteoporosis on osteoblasts and to examine the roles of β‑ecdysterone (β‑Ecd) involved. In the present study, an in vivo model of osteoporosis was established through the subcutaneous implantation of prednisolone (PRED) into Sprague‑Dawley rats, with or without a subcutaneous injection of β‑Ecd (5 or 10 mg/kg body weight)...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29136958/mir-182-5p-regulates-bcl2l12-and-bcl2-expression-in-acute-myeloid-leukemia-as-a-potential-therapeutic-target
#3
Suwei Zhang, Qiaoxin Zhang, Ganggang Shi, Jun Yin
The importance of microRNAs (miRNAs) are shown during various cancers including acute myeloid leukemia (AML). MiR-182-5p functions as an oncogene or a potential suppressive miRNA in cancers, but its expression and function in AML is unknown. The purpose is to investigate the roles of miR-182-5p in AML in this study. MiR-182-5p was examined in the blood samples of AML and it was found that miR-182-5p expression levels were higher in AML tissues than it in their normal controls, so did in the AML cells. BCL2L12 and BCL2 were predicted as target genes of miR-182-5p and verified using luciferase reporter assay...
November 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29128415/the-p38-mitogen-activated-protein-kinase-regulates-%C3%AE-amyloid-protein-internalization-through-the-%C3%AE-7-nicotinic-acetylcholine-receptor-in-mouse-brain
#4
Kai-Ge Ma, Jia Lv, Wei-Na Yang, Ke-Wei Chang, Xiao-Dan Hu, Li-Li Shi, Wan-Ying Zhai, Hang-Fan Zong, Yi-Hua Qian
Alzheimer's disease (AD) is one of the most devastating neurodegenerative disorders. Intracellular β-amyloid protein (Aβ) is an early event in AD. It induces the formation of amyloid plaques and neuron damage. The α7 nicotinic acetylcholine receptor (α7nAChR) has been suggested to play an important role in Aβ caused cognition. It has high affinity with Aβ and could mediate Aβ internalization in vitro. However, whether in mouse brain the p38 MAPK signaling pathway is involved in the regulation of the α7nAChR mediated Aβ internalization and their role in mitochondria remains little known...
November 9, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/29118912/high-expression-of-fibronectin-1-suppresses-apoptosis-through-the-nf-%C3%AE%C2%BAb-pathway-and-is-associated-with-migration-in-nasopharyngeal-carcinoma
#5
Jinting Wang, Lian Deng, Junpeng Huang, Rui Cai, Xiongjie Zhu, Faquan Liu, Qien Wang, Jiren Zhang, Yanfang Zheng
Fibronectin 1 (FN1) is a member of the glycoprotein family located on chromosome 2q35. It has been reported that FN1 is upregulated in many tumors, and its expression is negatively related to the prognosis and survival of cancer patients. Through data analysis, we found that FN1 is upregulated in nasopharyngeal carcinoma (NPC). This study aimed to investigate how FN1 expression affects NPC cell behavior. In this study, we downregulated FN1 in two NPC cell lines, 5-8F (EBV-) and C666-1 (EBV+), and evaluated invasion, migration and apoptosis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29115490/microrna%C3%A2-206-contributes-to-the-progression-of-steroid%C3%A2-induced-avascular-necrosis-of-the-femoral-head-by-inducing-osteoblast-apoptosis-by-suppressing-programmed-cell-death-4
#6
Zaiheng Zhang, Anmin Jin, Denglu Yan
The expression of microRNA‑206 (miR‑206) is aberrantly induced in steroid‑induced avascular necrosis of femoral head (SANFH). Therefore, investigating the function of miR‑206 in SANFH and uncovering the functional mechanism associated with the condition will promote the understanding and treatment of the disease. The purpose of the present study was to investigate the pro‑osteoclasteogenic effect of miR‑206 that occurs through regulation of programmed cell death 4 (PDCD4). The expression of miR‑206 and PDCD4 was analyzed in the clinical SANFH specimens...
November 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29113197/acidic-stress-induces-apoptosis-and-inhibits-angiogenesis-in-human-bone-marrow-derived-endothelial-progenitor-cells
#7
Shuai Huang, Peiheng He, Dongliang Xu, Jinglei Li, Xinsheng Peng, Yubo Tang
Bone marrow-derived endothelial progenitor cells (BM-EPCs) are exposed to acidotic environments in a variety of physiological and pathological conditions, including in tumors. However, the effect of acidosis on the function of BM-EPCs is still not fully understood. In the present study, BM-EPCs were isolated and cultured at an extracellular pH (pHe) of 6.5 or pHe 7.4 in vitro prior to various experiments being performed. Cellular proliferation, migration and tube formation ability were detected by DNA content quantification, Transwell assay and Matrigel-based angiogenesis assay...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29103194/astragalus-polysaccharides-increase-the-sensitivity-of-skov3-cells-to-cisplatin
#8
Caihong Li, Li Hong, Cheng Liu, Jie Min, Ming Hu, Wenjun Guo
BACKGROUND: Multidrug resistance in malignant tumours hinders the treatment of tumours. Studies showed that astragalus polysaccharides (APS), one major active ingredient of astragalus, enhanced the sensitivity of non-small cell lung cancer and liver cancer cells to chemotherapeutic drug. However, the effect of APS on ovarian cancer is still unclear. In this study, we will examine the sensitizing effect of APS on SKOV3 cells to cisplatin and explore the possible mechanism. METHODS: MTT assay was employed to examine the viability of SKOV3 after treatment with APS and cisplatin...
November 4, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29093665/pi3k-and-inhibitor-of-apoptosis-proteins-modulate-gentamicin-induced-hair-cell-death-in-the-zebrafish-lateral-line
#9
Heather Wiedenhoft, Lauren Hayashi, Allison B Coffin
Inner ear hair cell death leads to sensorineural hearing loss and can be a direct consequence of aminoglycoside antibiotic treatment. Aminoglycosides such as gentamicin are effective therapy for serious Gram-negative bacterial infections such as some forms of meningitis, pneumonia, and sepsis. Aminoglycosides enter hair cells through mechanotransduction channels at the apical end of hair bundles and initiate intrinsic cell death cascades, but the precise cell signaling that leads to hair cell death is incompletely understood...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29089079/-18%C3%AE-glycyrrhetinic-acid-piperazine-derivative-a30-inhibits-the-proliferation-of-smmc-7721-hepatoma-cells
#10
Like Zhong
Objective To investigate the mechanism of 18β-glycyrrhetinic acid (GA) piperazine derivative A30 on the antiproliferation of hepatocellular carcinoma SMMC-7721 cells in vitro. Methods The experiment included three groups: control group, 18β-GA group and A30 group. The proliferation activity was detected by MTT assay. Cell apoptosis and the change in the cycle of SMMC-7721 cells were evaluated by flow cytometry. Western blotting was used to observe the expressions of Bcl2 and caspase-8. Results The proliferation of SMMC-7721 cells was inhibited by A30 at the concentration of 2-128 μg/mL in a dose-dependent manner...
September 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29081818/curcumin-induces-autophagy-apoptosis-and-cell-cycle-arrest-in-human-pancreatic-cancer-cells
#11
Yaping Zhu, Shurui Bu
OBJECTIVE: Curcumin is an active extract from turmeric. The aim of this study was to identify the underlying mechanism of curcumin on PCa cells and the role of autophagy in this process. METHODS: The inhibitory effect of curcumin on the growth of PANC1 and BxPC3 cell lines was detected by CCK-8 assay. Cell cycle distribution and apoptosis were tested by flow cytometry. Autophagosomes were tested by cell immunofluorescence assay. The protein expression was detected by Western blot...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29076273/systemic-delivery-of-sirna-by-dna-nanoparticles-targeting-bcl2-in-cancer-cell-growth
#12
Mohammad Aminur Rahman, Pengfei Wang, Zhixiang Zhao, Dongsheng Wang, Sreenivas Nannapaneni, Chao Zhang, Zhengjia Chen, Christopher C Griffith, Selwyn J Hurwitz, Zhuo G Chen, Yonggang Ke, Dong Moon Shin
siRNA is a promising molecular tool for cancer therapy, but its clinical success is limited by the lack of robust in vivo delivery systems. Rationally designed DNA nanoparticles (DNPs) have emerged as facile delivery vehicles since their physicochemical properties can be precisely controlled. Nonetheless, few studies have used DNPs to deliver siRNAs in vivo, and none has demonstrated therapeutic efficacy. Herein, we constructed a number of DNPs of rectangular and tubular shapes with varied dimensions using the modular DNA brick method for the systemic delivery of siRNA targeting an antiapoptotic protein Bcl2...
October 26, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29072699/cited2-affects-leukemic-cell-survival-by-interfering-with-p53-activation
#13
Katharina Mattes, Gerbrig Berger, Marjan Geugien, Edo Vellenga, Hein Schepers
CITED2 (CBP/p300-interacting-transactivator-with-an-ED-rich-tail 2) is a regulator of the acetyltransferase CBP/p300 and elevated CITED2 levels are shown in a number of acute myeloid leukemia (AML). To study the in vivo role of CITED2 in AML maintenance, AML cells were transduced with a lentiviral construct for RNAi-mediated knockdown of CITED2. Mice transplanted with CITED2-knockdown AML cells (n=4) had a significantly longer survival compared to mice transplanted with control AML cells (P<0.02). In vitro, the reduction of CITED2 resulted in increased p53-mediated apoptosis and CDKN1A expression, whereas BCL2 levels were reduced...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29072681/demethylzeylasteral-inhibits-cell-proliferation-and-induces-apoptosis-through-suppressing-mcl1-in-melanoma-cells
#14
Yuzu Zhao, Jiang He, Jun Li, Xingzhi Peng, Xianxing Wang, Zhen Dong, Erhu Zhao, Yaling Liu, Zonghui Wu, Hongjuan Cui
Demethylzeylasteral is one of the extracts of Tripterygium wilfordii Hook F, which plays important roles in multiple biological processes such as inflammation inhibition, as well as immunosuppression. However, anti-cancer function and the underlying mechanisms of demethylzeylasteral in melanoma cells remain unclear. In this study, we demonstrate that demethylzeylasteral has an anti-tumor property in melanoma cells. Demethylzeylasteral not only inhibits cell proliferation through cell cycle arrest at S phase, but also induces cell apoptosis in melanoma cells...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29070462/-role-of-aquaporin-5-in-regulating-colorectal-cancer-cell-growth-in-vitro
#15
Shuo Chen, Tao Shan, Li Wang, Xi Chen, Xi-Juan Cui, Xian-Ni Gao
OBJECTIVE: To study the role of aquaporin 5(AQP5) in regulating the cell proliferation and apoptosis of human colorectal cancer cells and explore the possible mechanism. METHODS: A small interfering RNA (siRNA) targeting AQP5 was used to suppress endogenous AQP5 expression in the human colorectal cancer cell lines COLO 205 and SW480, and the transfection efficiency of AQP5 siRNA was determined using immunofluorescence assay and PCR. The changes in the proliferation of the transfected cells was evaluated with MTT assay, and the cell apoptosis was analyzed using Annexin V-FITC/PI and TUNEL assays; the changes of Bax and Bcl2 expressions in the cells were determined using RTPCR and Western blotting...
October 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29068538/novel-1-3-5-triazine-derivatives-exert-potent-anti-cervical-cancer-effects-by-modulating-bax-bcl2-and-caspases-expression
#16
Xiwen Wang, Yuexiong Yi, Qiongying Lv, Juan Zhang, Kejia Wu, Wanrong Wu, Wei Zhang
The present study aimed to develop novel 1,3,5-triazine derivatives as potent anti-cervical cancer agents. The compounds were synthesised in short steps with an excellent yield and characterised via various spectroscopic and analytical methods. A structure-activity relationship study suggested that electron-withdrawing substituents showed greater anticancer activity than electron-donating groups. Compound 7p (p-fluoro) showed the highest activity against cervical cancer cells. In a nude mouse xenograft model inoculated with HeLa cells, 7p showed dose-dependent inhibition of cervical tumour growth...
October 25, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29066528/acute-nutrient-treatment-causes-alterations-in-intra-follicular-antioxidation-and-akt-signaling
#17
Haitao Nie, Yixuan Guo, Xiaolei Yao, Tiewei Ma, KaiPing Deng, Zheng Wang, Guomin Zhang, Lingwei Sun, Zi Yu Wang, HengChang Wang, Feng Wang
This study aimed to determine if short-term nutrient alteration affects 1) ovary morphology, 2) plasma and ovarian antioxidant capability, and 3) cell apoptosis and Akt signaling within the ovary. After estrus synchronization, 24 Hu sheep were assigned to three groups based on the nutrient requirement recommended for maintenance (M): 1×M (Control), 1.5×M (S), and 0.5×M (R) during day 7 to 14 of their estrous cycle. The results indicated that undernourishment significantly increased the counts and volume of follicles <2...
October 24, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29065794/prolonged-exposure-of-resveratrol-induces-reactive-superoxide-species-independent-apoptosis-in-murine-prostate-cells
#18
Sanjay Kumar, James Stokes, Udai P Singh, Karyn Scissum-Gunn, Rajesh Singh, Upender Manne, Manoj K Mishra
Nitric oxide, a signaling molecule, inhibits mitochondrial respiration by binding with cytochrome c oxidase, resulting in elevated production of reactive superoxide species (reactive oxygen and nitrogen) in the mitochondria and increased susceptibility to cell death. Generation of mitochondrial superoxide species can be suppressed by natural compounds such as resveratrol, a dietary polyphenol found in the skin of red fruits. In various cancer cells, resveratrol shows anti-oxidant and cancer preventive properties...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29065414/protective-effects-of-sonic-hedgehog-against-ischemia-reperfusion-injury-in-mouse-skeletal-muscle-via-akt-mtor-p70s6k-signaling
#19
Qiu Zeng, Qining Fu, Xuehu Wang, Yu Zhao, Hong Liu, Zhui Li, Fenghe Li
BACKGROUND/AIMS: Skeletal muscle ischemia/reperfusion (I/R) injury is a common and severe disease. Sonic hedgehog (Shh) plays a critical role in post-natal skeletal muscle regeneration. In the present study, the role of Shh in skeletal muscle I/R injury and the mechanisms involved were investigated. METHODS: The expression of Shh, AKT/mTOR/p70S6K and apoptosis pathway components were evaluated following tourniquet-induced skeletal muscle I/R injury. Then, mice were subjected to systemic administration of cyclopamine or one-shot treatment of a plasmid encoding the human Shh gene (phShh) to examine the effects of Shh on I/R injury...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29063678/molecular-profiling-and-combinatorial-activity-of-cct068127-a-potent-cdk2-and-cdk9-inhibitor
#20
Steven R Whittaker, Clare Barlow, Mathew P Martin, Caterina Mancusi, Steve Wagner, Annette Self, Elaine Barrie, Robert Te Poele, Swee Sharp, Nathan Brown, Stuart Wilson, Wayne Jackson, Peter M Fischer, Paul A Clarke, Michael I Walton, Edward McDonald, Julian Blagg, Martin Noble, Michelle D Garrett, Paul Workman
Deregulation of the cyclin-dependent kinases (CDKs) has been implicated in the pathogenesis of multiple cancer types. Consequently, CDKs have garnered intense interest as therapeutic targets for the treatment of cancer. We describe herein the molecular and cellular effects of CCT068127, a novel inhibitor of CDK2 and CDK9. Optimised from the purine template of seliciclib, CCT068127 exhibits greater potency and selectivity against purified CDK2 and CDK9 and superior antiproliferative activity against human colon cancer and melanoma cell lines...
October 24, 2017: Molecular Oncology
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