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https://www.readbyqxmd.com/read/27821509/approach-to-the-diagnosis-and-treatment-of-high-grade-b-cell-lymphomas-with-myc-and-bcl2-and-or-bcl6-rearrangements
#1
Pierre Sesques, Nathalie A Johnson
High grade B cell lymphomas (HGBL) with MYC and BCL2 and/or BCL6 rearrangements, so-called "double hit" lymphomas (HGBL-DH), are aggressive lymphomas that form a separate provisional entity in the 2016 revised World Heath Organization Classification of Lymphoid Tumours. Fluorescence in situ hybridization (FISH) will be required to identify HGBL-DH and will re-classify a subset of diffuse large B cell lymphomas (DLBCL) and HGBL with features intermediate between DLBCL and Burkitt lymphoma into this new category...
November 7, 2016: Blood
https://www.readbyqxmd.com/read/27775850/clinicopathological-characteristics-and-genomic-profile-of-primary-sinonasal-tract-diffuse-large-b-cell-lymphoma-dlbcl-reveals-gain-at-1q31-and-rgs1-encoding-protein-high-rgs1-immunohistochemical-expression-associates-with-poor-overall-survival-in-dlbcl-nos
#2
Joaquim Carreras, Yara Yukie Kikuti, Sílvia Beà, Masashi Miyaoka, Shinichiro Hiraiwa, Haruka Ikoma, Ryoko Nagao, David Martin-Garcia, Itziar Salaverria, Ai Sato, Ichiki Akifumi, Giovanna Roncador, Juan F Garcia, Kiyoshi Ando, Elias Campo, Naoya Nakamura
AIMS AND METHODS: We aimed to define the clinicopathological characteristics of 29 primary sinonasal diffuse large B-cell lymphoma (DLBCL(sn) ) in a series of 240 DLBCL(all ()(NOS)()) including DLBCL(sn) training set (n=11) and validation set (n=18), and DLBCL(non-sn) (n=211). RESULTS: In the training set 82% had non-GCB phenotype and 18% were EBER(+) . The genomic profile showed gains((+)) of 1q21.3q31.2 (55%), 10q24.1 (46%), 11q14.1 (46%) and 18q12.1q23 (46%); losses((-)) of 6q26q27 (55%) and 9p21...
October 24, 2016: Histopathology
https://www.readbyqxmd.com/read/27773559/immunophenotypic-and-genetic-characteristics-of-diffuse-large-b-cell-lymphoma-in-taiwan
#3
Sheng-Tsung Chang, Shang-Wen Chen, Chung-Han Ho, Chun-Chi Kuo, Seiji Sakata, Kengo Takeuchi, Shih-Sung Chuang
BACKGROUND/PURPOSE: Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma type. The immunophenotypic and genetic features of DLBCL in Taiwan have not been characterized. METHODS: In this study, we performed immunohistochemical analysis and interphase fluorescence in situ hybridization (FISH) using tissue microarray sections to investigate a cohort of unselected DLBCL cases in a single institution in Taiwan from 1990 to 2010. RESULTS: Of the 153 cases investigated, CD10, bcl-6, and MUM1 were expressed in 16...
October 20, 2016: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/27628560/-double-hit-follicular-lymphoma-with-bcl2-and-myc-translocations
#4
Mirei Horiuchi, Hoyuri Fuseya, Minako Tsutsumi, Yoshiki Hayashi, Kiyoyuki Hagihara, Hiroshi Kanashima, Takafumi Nakao, Yuko Fukushima, Takeshi Inoue, Takahisa Yamane
Double-hit lymphomas are rare tumors that are defined by a chromosomal breakpoint affecting the MYC/8q24 locus in combination with another recurrent breakpoint, mainly a t(14; 18)(q32;q21)involving BCL2. We report a case of a 38-yearold woman with a 2-month history of abdominaldistention. 18F-FDG PET showed multiple positive systemic lymph nodes, positive peritoneum, and multiple positive intra-abdominal masses. Histopathology results of the cervical lymph node were compatible with double-hit follicular lymphoma(Grade 3A)because fluorescence in situ hybridization(FISH)demonstrated both MYC rearrangement and BCL2 gene fusion...
September 2016: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/27616053/ifr4-mum1-positive-lymphoma-in-waldeyer-ring-with-co-expression-of-cd5-and-cd10
#5
Lei Chen, Lika'a Fasih Y Al-Kzayer, Tingting Liu, Norimoto Kobayashi, Yozo Nakazawa, Kenichi Koike
IRF4/MUM1-positive lymphoma is a new subgroup of germinal center-derived B-cell lymphoma, predominantly involving the Waldeyer ring (WR) in children. CD5 expression is rare in these lymphomas. We report a 7-year-old Chinese male with B-cell lymphoma. Evaluation of his specimen by morphology, immunohistochemistry, and FISH analysis demonstrated IRF4/MUM1-positive lymphoma with strong and extensive CD5 and CD10 positivity. Despite the lack of t(14;18)(q32;q21) rearrangement, BCL2 protein was expressed. Our report highlights the clinicopathologic features of IFR4/MUM1-positive lymphoma in WR with co-expression of CD5 and CD10, and thereby provides insight into this newly recognized disease entity...
September 12, 2016: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/27561114/bisphenol-a-induces-spermatocyte-apoptosis-in-rare-minnow-gobiocypris-rarus
#6
Yingying Zhang, Mengqian Cheng, Lang Wu, Guo Zhang, Zaizhao Wang
Bisphenol A (BPA) is an endocrine disruptor, and could induce germ cells apoptosis in the testis of mammals. But whether it could affect fish in the same mechanism has not' been studied till now. In the present study, to investigate the influence of BPA on testis germ cells in fish, adult male rare minnow Gobiocypris rarus were exposed to 225μgL(-1) (0.99μM) BPA for 1, 3 and 9 weeks. Through TdT-mediated dUTP nick end labeling (TUNEL) and transmission electron microscope (TEM) analysis, we found that the amount of apoptotic spermatocytes significantly increased in a time dependent manner following BPA exposure...
October 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27468869/incidence-of-preleukemic-fusion-genes-in-healthy-subjects
#7
P Kosik, M Skorvaga, I Belyaev
The diagnostics of leukemia relies upon multi-parametric approach involving a number of different pathology disciplines such as flow cytometry, histopathology, cytogenetics and molecular genetics [fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR)]. Childhood leukemia is often determined by the presence of specific chromosomal translocation that entails the generation of preleukemic fusion genes (PFG). In the last two decades, several studies have reported observations that PFG are present in healthy population and not necessarily result in leukemia...
2016: Neoplasma
https://www.readbyqxmd.com/read/27390596/clinical-significance-of-double-hit-and-double-protein-expression-in-primary-gastric-b-cell-lymphomas
#8
Miaoxia He, Keting Chen, Suhong Li, Shimin Zhang, Jianming Zheng, Xiaoxia Hu, Lei Gao, Jie Chen, Xianmin Song, Weiping Zhang, Jianmin Wang, Jianmin Yang
BACKGROUND AND AIMS: Primary gastric B-cell lymphoma is the second most common malignancy of the stomach. There are many controversial issues about its diagnosis, treatment and clinical management. "Double-hit" and "double-protein" involving gene rearrangement and protein expression of c-Myc and bcl2/bcl6 are the most used terms to describe DLBCL poor prognostic factors in recent years. However, very little is known about the role of these prognostic factors in primary gastric B-cell lymphomas...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/27179425/ability-of-lactobacillus-plantarum-lipoteichoic-acid-to-inhibit-vibrio-anguillarum-induced-inflammation-and-apoptosis-in-silvery-pomfret-pampus-argenteus-intestinal-epithelial-cells
#9
Quanxin Gao, Qian Gao, Minghua Min, Chenjie Zhang, Shiming Peng, Zhaohong Shi
Lipoteichoic acid (LTA) is a major constituent of the cell wall of Gram-positive bacteria. The structure and immunomodulation of LTA vary greatly between different species. LTA from Lactobacillus plantarum has been shown to exert anti-pathogenic effects. Vibrio anguillarum is a major causative agent of vibriosis, one of the most prevalent fish diseases. The purpose of this study was to examine the effects of L. plantarum LTA on V. anguillarum growth, adhesion, and induced inflammation and apoptosis in intestinal epithelial cells of silvery pomfret (Pampus argenteus)...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27113350/venetoclax-approved-for-cll
#10
(no author information available yet)
Venetoclax, a BCL2 inhibitor, has been approved for patients with chronic lymphocytic leukemia (CLL) who lack part of chromosome 17. The FDA also approved a companion diagnostic, the Vysis CLL FISH probe kit, to detect the 17p deletion in patients' peripheral blood.
June 2016: Cancer Discovery
https://www.readbyqxmd.com/read/27108554/-follicular-and-mantle-cell-lymphoma-diagnosed-in-biopsies-of-gastroenterocolic-region
#11
Lukáš Plank, Tomáš Balhárek, Peter Szépe
The authors present a retrospective analysis of follicular lymphomas (FL) and mantle cell lymphomas (MCL) diagnosed according to the WHO classification (2008) in consecutive biopsies of GI organs in a period of 11 years. The series includes 18 patients with FL verified in 22 biopsies and 44 patients with MCL diagnosed in 54 biopsies. FL represented always a solitary tumor, most often - up to ¾ of all the cases - of a small intestine, more often in its jejunoileal than duodenal parts. The biopsies were obtained almost equally by endoscopical approach, they were usually mucosal and rarely polypectomic, as well as by surgical resections (54,5 % and 45,5 % of the cases respectively)...
2016: Ceskoslovenská Patologie
https://www.readbyqxmd.com/read/27041665/in%C3%A2-vitro-effects-of-metals-on-isolated-head-kidney-and-blood-leucocytes-of-the-teleost-fish-sparus-aurata-l-and-dicentrarchus-labrax-l
#12
Patricia Morcillo, José Meseguer, M Ángeles Esteban, Alberto Cuesta
The in vitro use of fish leucocytes to test the toxicity of aquatic pollutants, and particularly the immutoxicological effects, could be a valuable alternative to fish bioassays but has received little attention. In this study, head-kidney and peripheral blood leucocytes (HKLs and PBLs, respectively) from gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed to Cd, MeHg (methylmercury), Pb or As for 24 h being evaluated the resulting cytotoxicity. Exposure to metals produced a dose-dependent reduction in the viability, and MeHg showed the highest toxicity followed by Cd, As and Pb...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26976014/cytotoxicity-and-alterations-at-transcriptional-level-caused-by-metals-on-fish-erythrocytes-in-vitro
#13
Patricia Morcillo, Diego Romero, José Meseguer, M Ángeles Esteban, Alberto Cuesta
The in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. In this study, erythrocytes from marine gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed for 24 h to Cd, Hg, Pb and As and the resulting cytotoxicity was evaluated. Exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxicity followed by MeHg, Cd, As and Pb...
June 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/26910115/stratifying-diffuse-large-b-cell-lymphoma-patients-treated-with-chemoimmunotherapy-gcb-non-gcb-by-immunohistochemistry-is-still-a-robust-and-feasible-marker
#14
Ana Batlle-López, Sonia González de Villambrosía, Mazorra Francisco, Sefora Malatxeberria, Anabel Sáez, Carlos Montalban, Lydia Sánchez, Juan Garcia, Eva González-Barca, Andrés López-Hernández, M C Ruiz-Marcellan, Manuela Mollejo, Carlos Grande, Kristy L Richards, Eric D Hsi, Alexandar Tzankov, Carlo Visco, Zijun Y Xu-Monette, Xin Cao, Ken H Young, Miguel Ángel Piris, Eulogio Conde, Santiago Montes-Moreno
Diffuse large B cell lymphoma (DLBCL) is a heterogeneous group of aggressive lymphomas that can be classified into three molecular subtypes by gene expression profiling (GEP): GCB, ABC and unclassified. Immunohistochemistry-based cell of origin (COO) classification, as a surrogate for GEP, using three available immunohistochemical algorithms was evaluated in TMA-arranged tissue samples from 297 patients with de novo DLBCL treated by chemoimmunotherapy (R-CHOP and R-CHOP-like regimens). Additionally, the prognostic impacts of MYC, BCL2, IRF4 and BCL6 abnormalities detected by FISH, the relationship between the immunohistochemical COO classification and the immunohistochemical expression of MYC, BCL2 and pSTAT3 proteins and clinical data were evaluated...
April 5, 2016: Oncotarget
https://www.readbyqxmd.com/read/26834954/current-challenges-and-novel-treatment-strategies-in-double-hit-lymphomas
#15
REVIEW
Mary Ann Anderson, Alpha Tsui, Meaghan Wall, David C S Huang, Andrew W Roberts
High-grade B-cell lymphomas with recurrent chromosomal break points have been termed 'double hit lymphoma' (DHL). The most commonly seen DHL is diffuse large B-cell lymphoma (DLBCL) with t(14;18) and t(8;14) or t(8;22) resulting in overexpression of BCL2 and MYC, respectively. The increased proliferation due to MYC overexpression, without the ability for an apoptotic brake as a result of BCL2 overexpression, results in 'the perfect storm of oncogenesis'. Thus this disease presents a number of diagnostic and therapeutic challenges for the hematologist...
February 2016: Therapeutic Advances in Hematology
https://www.readbyqxmd.com/read/26762526/intrafollicular-epstein-barr-virus-positive-large-b-cell-lymphoma-a-variant-of-germinotropic-lymphoproliferative-disorder
#16
Luisa Lorenzi, Silvia Lonardi, Murad H M Essatari, Vilma Pellegrini, Simona Fisogni, Anna Gazzola, Claudio Agostinelli, William Vermi, Giuseppe Rossi, Giovannino Massarelli, Stefano A Pileri, Fabio Facchetti
Germinotropic lymphoproliferative disorders were previously described as localized disorders associated with coinfection by human herpes virus 8 and Epstein-Barr virus and characterized by good clinical outcome. We report the clinical, morphological, phenotypical, and molecular features of three cases of a hitherto unreported variant of Epstein-Barr virus (EBV)-positive, human herpes virus 8 (HHV8)-negative large B cell lymphoma with exclusive intrafollicular localization. All cases occurred in elderly individuals (63, 77, and 65 years old; one male, two females) without obvious immunedeficiency, who presented with high stage disease...
April 2016: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/26727417/malignant-hematopoietic-cell-lines-in-vitro-models-for-double-hit-b-cell-lymphomas
#17
Hans G Drexler, Sonja Eberth, Stefan Nagel, Roderick A F MacLeod
Mature B-cell lymphomas with concurrent rearrangements of MYC and BCL2 (more rarely BCL6), "double-hit lymphomas" (DHLs), form a heterogeneous group. Recent studies have shown that DHLs often present with an aggressive clinical course and a poor prognosis with standard therapy. This distinct clinical entity would benefit from more detailed pathobiological characterization to develop improved treatment options. Lymphoma cell lines are important and informative research tools. Several cell lines have been established from B-cell lymphoma patients harboring MYC rearrangements combined with either (double-hit) or both (triple-hit) BCL2 or BCL6 translocations...
May 2016: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/26690614/chronic-lymphocytic-leukemia-cll-then-and-now
#18
REVIEW
Kanti R Rai, Preetesh Jain
The field of chronic lymphocytic leukemia (CLL) has witnessed considerable change since the time clinical staging was introduced in clinical practice in 1975. Over the years, the prognostication in CLL has expanded with the addition in late 90s of mutational status of variable region of immunoglobulin heavy chain (IGHV), and chromosomal analyses using fluorescent in situ hybridization (FISH). More recently, stereotypy of BCR (B cell receptor) and whole exome sequencing (WES) based discovery of specific mutations such as NOTCH1, TP53, SF3B1, XPO-1, BIRC3, ATM, and RPS15 further refined the current prognostication system in CLL...
March 2016: American Journal of Hematology
https://www.readbyqxmd.com/read/26515759/establishment-and-characterization-of-a-novel-myc-bcl2-double-hit-diffuse-large-b-cell-lymphoma-cell-line-rc
#19
Lan V Pham, Gary Lu, Archito T Tamayo, Juan Chen, Pramoda Challagundla, Jeffrey L Jorgensen, L Jeffrey Medeiros, Richard J Ford
BACKGROUND: Diffuse large B cell lymphoma (DLBCL) is the most common type of lymphoid malignancy worldwide. Approximately 5 % of cases of DLBCL are so-called double-hit lymphomas (DHL), defined by a chromosomal translocation or rearrangement involving MYC/8q24.2 in combination with another recurrent breakpoint, usually BCL2/18q21.3. Patients with MYC/BCL2 DHL are resistant to standard front-line therapy, and currently, there is no consensus for a therapeutic strategy to treat these patients...
2015: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/26457084/diffuse-large-b-cell-lymphoma-with-extensive-cutaneous-relapse
#20
Umit Yavuz Malkan, Gursel Gunes, Okan Yayar, Haluk Demiroglu
Herein, we aimed to report a diffuse large B cell lymphoma (DLBCL) case that had extensive cutaneous relapse with no skin involvement previously. A 59-year-old man presented to hospital in April 2014 with fatigue, anorexia, fever, and anemia. Cervical lymph node biopsy revealed CD20+, BCL2+, MUM1+, BCL6+ high grade B lymphoproliferative neoplasm. After FISH investigation, he was diagnosed as DLBCL. He was given 7 cycles of R-CHOP and achieved remission. However, in November 2014, he had emerging skin lesions that cover nearly all of his body...
2015: Case Reports in Medicine
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