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Malaria biomarker

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https://www.readbyqxmd.com/read/27911910/molecular-markers-of-radiation-induced-attenuation-in-intrahepatic-plasmodium-falciparum-parasites
#1
Miranda S Oakley, Nitin Verma, Hong Zheng, Vivek Anantharaman, Kazuyo Takeda, Yamei Gao, Timothy G Myers, Phuong Thao Pham, Babita Mahajan, Nirbhay Kumar, Davison Sangweme, Abhai K Tripathi, Godfree Mlambo, L Aravind, Sanjai Kumar
Experimental immunization with radiation attenuated sporozoites (RAS) and genetically attenuated sporozoites has proved to be a promising approach for malaria vaccine development. However, parasite biomarkers of growth attenuation and enhanced immune protection in response to radiation remain poorly understood. Here, we report on the effect of an attenuating dose of γ-irradiation (15 krad) on the Plasmodium falciparum sporozoite (PfSPZ) ultrastructure by electron microscopy, growth rate of liver stage P. falciparum in liver cell cultures, and genome-wide transcriptional profile of liver stage parasites by microarray...
2016: PloS One
https://www.readbyqxmd.com/read/27905921/systematic-review-of-the-role-of-angiopoietin-1-and-angiopoietin-2-in-plasmodium-species-infections-biomarkers-or-therapeutic-targets
#2
Gerdie M de Jong, Jasper J Slager, Annelies Verbon, Jaap J van Hellemond, Perry J J van Genderen
BACKGROUND: Levels of both angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) correlate with malaria disease severity and are proposed as biomarkers and possible therapeutic targets. To establish their role in malaria, a systematic review was performed of the literature on Ang-1 and Ang-2 with regard to their potential as biomarkers in malaria and discuss their possible place in adjuvant treatment regimens. METHODS: Ten electronic databases were systematically searched to identify studies investigating Ang-1 and Ang-2 in human and murine malaria in both clinical and experimental settings...
December 1, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27888627/identification-of-differentially-expressed-genes-in-the-development-of-osteosarcoma-using-rna-seq
#3
Yihao Yang, Ya Zhang, Xin Qu, Junfeng Xia, Dongqi Li, Xiaojuan Li, Yu Wang, Zewei He, Su Li, Yonghong Zhou, Lin Xie, Zuozhang Yang
OBJECTIVE: Osteosarcoma (OS) is a malignant bone tumor with high morbidity in young adults and adolescents. This study aimed to discover potential early diagnosis biomarkers in OS. RESULTS: In total, 111 differentially expressed genes (DEGs) were identified in primary OS compared with normal controls and 235 DEGs were identified in metastatic OS compared with primary OS. AURKB and PPP2R2B were the significantly up-regulated and down-regulated hub proteins, respectively, in the PPI protein-protein network (PPI) network of primary OS...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27871325/impact-of-placental-plasmodium-falciparum-malaria-infection-on-the-cameroonian-maternal-and-neonate-s-plasma-levels-of-some-cytokines-known-to-regulate-t-cells-differentiation-and-function
#4
Jean Claude Djontu, Stalone Siewe Siewe, Yolande Delphine Mpeke Edene, Benderli Christine Nana, Edwige Vanessa Chomga Foko, Jude Daiga Bigoga, Rose F G Leke, Rosette Megnekou
BACKGROUND: The impact of placental malaria (PM) infection on the expression profile of some cytokines known to regulate T cell differentiation and function and their influence on birth weight remain unclear. Moreover, there are no reports showing the relationship between PM and IL-27 or IL-28A. This study therefore sought to investigate whether placental P. falciparum infection alters the expression profile of the cytokines IL-28A, IL-27, IL-17E and IL-6 in mothers and their new born...
November 21, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27829560/enzyme-free-electrochemical-immunosensor-based-on-methylene-blue-and-the-electro-oxidation-of-hydrazine-on-pt-nanoparticles
#5
Gorachand Dutta, Sureshbabu Nagarajan, Lisa J Lapidus, Peter B Lillehoj
Enzyme-free electrochemical sensors enable rapid, high sensitivity measurements without the limitations associated with enzyme reporters. However, the performance of non-enzymatic electrochemical sensors tends to suffer from slow electrode kinetics and poor signal stability. We report a new enzyme-free electrochemical immunosensor based on a unique competitive detection scheme using methylene blue (MB), hydrazine and platinum nanoparticles (Pt NPs). This scheme is coupled with a robust immunosandwich format employing a MB-labelled detection antibody as a non-enzymatic reporter...
November 3, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27806725/the-effect-of-malaria-and-anti-malarial-drugs-on-skeletal-and-cardiac-muscles
#6
REVIEW
Mauro Toledo Marrelli, Marco Brotto
Malaria remains one of the most important infectious diseases in the world, being a significant public health problem associated with poverty and it is one of the main obstacles to the economy of an endemic country. Among the several complications, the effects of malaria seem to target the skeletal muscle system, leading to symptoms, such as muscle aches, muscle contractures, muscle fatigue, muscle pain, and muscle weakness. Malaria cause also parasitic coronary artery occlusion. This article reviews the current knowledge regarding the effect of malaria disease and the anti-malarial drugs on skeletal and cardiac muscles...
November 2, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27774815/immunomic-approaches-for-antigen-discovery-of-human-parasites
#7
Kokouvi Kassegne, Eniola Michael Abe, Jun-Hu Chen, Xiao-Nong Zhou
Genetics combined with proteomics allows for a better understanding of parasite-host interactions and host immune responses. Immunomics elucidates that antigens are targets of induced or naturally acquired immunity (NAI), a promising solution to the challenge of eradicating human infections. High-throughput protein microarrays enhance rapid antigen discovery for the development of serodiagnostic tests/vaccines. Areas covered: This review systematically analyzes the emergence of protein microarrays as a powerful technology for parasite antigen discovery and subsequently summarizes some of the attributes and disadvantages of these approaches...
November 7, 2016: Expert Review of Proteomics
https://www.readbyqxmd.com/read/27748303/aetiology-outcomes-predictors-of-mortality-in-acute-respiratory-distress-syndrome-from-a-tertiary-care-centre-in-north-india
#8
Surendra K Sharma, Anunay Gupta, Ashutosh Biswas, Abhishek Sharma, Atul Malhotra, K T Prasad, Sreenivas Vishnubhatla, Sajal Ajmani, Hridesh Mishra, Manish Soneja, Shobha Broor
BACKGROUND & OBJECTIVES: Acute respiratory distress syndrome (ARDS) is a common disorder in critically ill patients and is associated with high mortality. There is a paucity of literature on this condition from developing countries. This prospective observational study was designed to find out the aetiology, outcomes and predictors of mortality in ARDS. METHODS: Sixty four consecutive patients who satisfied American-European Consensus Conference (AECC) definition of ARDS from medical Intensive Care Unit (ICU) of a tertiary care centre in New Delhi, India, were enrolled in the study...
June 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27703996/host-biomarkers-are-associated-with-response-to-therapy-and-long-term-mortality-in-pediatric-severe-malaria
#9
Andrea L Conroy, Michael Hawkes, Chloe R McDonald, Hani Kim, Sarah J Higgins, Kevin R Barker, Sophie Namasopo, Robert O Opoka, Chandy C John, W Conrad Liles, Kevin C Kain
Background.  Host responses to infection are critical determinants of disease severity and clinical outcome. The development of tools to risk stratify children with malaria is needed to identify children most likely to benefit from targeted interventions. Methods.  This study investigated the kinetics of candidate biomarkers of mortality associated with endothelial activation and dysfunction (angiopoietin-2 [Ang-2], soluble FMS-like tyrosine kinase-1 [sFlt-1], and soluble intercellular adhesion molecule-1 [sICAM-1]) and inflammation (10 kDa interferon γ-induced protein [CXCL10/IP-10] and soluble triggering receptor expressed on myeloid cells-1 [sTREM-1]) in the context of a randomized, double-blind, placebo-controlled, parallel-arm trial evaluating inhaled nitric oxide versus placebo as adjunctive therapy to parenteral artesunate for severe malaria...
September 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/27659695/development-of-an-indicator-displacement-based-detection-of-malaria-targeting-hrp-ii-as-biomarker-for-application-in-point-of-care-settings
#10
Babina Chakma, Priyamvada Jain, Naveen K Singh, Pranab Goswami
A novel label free spectrophotometric detection of malarial biomarker HRP-II following an indicator displacement assay has been developed. The assay is based on competitive displacement of murexide dye from its complex with Ni(2+) by HRP-II present in serum samples. The binding constant (Kd) discerned for the dye and HRP-II to Ni(2+) were 1.4 × 10(-6) M(-1) and 6.8 × 10(-9) M(-1), respectively. The progress of the reaction could be monitored from the change of color from orange (∼λ482 nm) to pink (∼λ515 nm) with the concomitant increase in HRP-II concentration in the mixture...
October 18, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27647324/absence-of-apolipoprotein-e-protects-mice-from-cerebral-malaria
#11
Fikregabrail Aberra Kassa, Kristin Van Den Ham, Anthony Rainone, Sylvie Fournier, Eric Boilard, Martin Olivier
Cerebral malaria claims the life of millions of people each year, particularly those of children, and is a major global public health problem. Thus, the identification of novel malaria biomarkers that could be utilized as diagnostic or therapeutic targets is becoming increasingly important. Using a proteomic approach, we previously identified unique biomarkers in the sera of malaria-infected individuals, including apolipoprotein E (ApoE). ApoE is the dominant apolipoprotein in the brain and has been implicated in several neurological disorders; therefore, we were interested in the potential role of ApoE in cerebral malaria...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27641435/tetrahydrobiopterin-supplementation-improves-phenylalanine-metabolism-in-a-murine-model-of-severe-malaria
#12
Matthew Sievers Alkaitis, Hans C Ackerman
Tetrahydrobiopterin (BH4) is an essential cofactor for both phenylalanine hydroxylase and nitric oxide synthase. Patients with severe malaria have low urinary BH4, elevated plasma phenylalanine, and impaired endothelium-dependent vasodilation, suggesting that BH4 depletion may limit phenylalanine metabolism and nitric oxide synthesis. We infected C57BL/6 mice with Plasmodium berghei ANKA to characterize BH4 availability and to investigate the effects of BH4 supplementation. P. berghei ANKA infection lowered BH4 in plasma, erythrocytes and brain tissue, but raised it in aorta and liver tissue...
September 19, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27634686/probucol-dramatically-enhances-dihydroartemisinin-effect-in-murine-malaria
#13
Aiko Kume, Dang Trinh Minh Anh, Mototada Shichiri, Noriko Ishida, Hiroshi Suzuki
BACKGROUND: Artemisinin-based combination therapy (ACT) has been adopted as national policy for the first-line treatment in large number of malaria-endemic regions. However, artemisinin-resistant parasites have emerged and are spreading, with slow-cleaning parasites being reported in patients treated with ACT. It means that more parasites are exposed to the partner drug alone and the risk of developing resistant parasites against the partner drug is increasing. Therefore, the development of a new method to enhance the effect of artemisinin is required...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27630842/simple-molecular-methods-for-early-detection-of-chloroquine-drug-resistance-in-plasmodium-vivax-and-plasmodium-falciparum
#14
Gurjeet Singh, Raksha Singh, Anant Dattatraya Urhehar
INTRODUCTION: Malaria is a human disease of which causes high morbidity and mortality. In Plasmodium falciparum malaria, the resistance to antimalarial drugs, especially chloroquine (CQ) is one of the paramount factors contributing to the global increase in morbidity and mortality, due to malaria. Hence, there is a need for detection of chloroquine drug resistance genes i.e., pfcrt-o (Plasmodium falciparum chloroquine resistance transporter-o) and pfmdr-1 (Plasmodium falciparum multidrug resistance-1) of P...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27612570/neutrophil-gelatinase-associated-lipocalin-ngal-predicts-the-occurrence-of-malaria-induced-acute-kidney-injury
#15
Marlies E van Wolfswinkel, Liese C Koopmans, Dennis A Hesselink, Ewout J Hoorn, Rob Koelewijn, Jaap J van Hellemond, Perry J J van Genderen
BACKGROUND: Acute kidney injury (AKI) is a frequently encountered complication of imported Plasmodium falciparum infection. Markers of structural kidney damage have been found to detect AKI earlier than serum creatinine-based prediction models but have not yet been evaluated in imported malaria. This pilot study aims to explore the predictive performance of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) for AKI in travellers with imported P. falciparum infection...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27608777/predicting-survival-in-ards
#16
EDITORIAL
Niteen D Karnik, Anish V Gupta
Acute respiratory distress syndrome (ARDS) is a fulminant clinical disorder of varied etiology, characterized by diffuse lung injury and severe hypoxemia. It is a leading cause of ICU admission and the associated high mortality has sparked a lot of research on etiology, outcome, scoring systems, mortality predictors, biomarkers including inflammatory cytokines and even genomics in ARDS. The previously used AECC (American European Consensus Conference) definition (1994) of ARDS was replaced by the recent Berlin definition (2012) so as to improve its validity and reliability...
November 2015: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27604417/the-association-between-naturally-acquired-igg-subclass-specific-antibodies-to-the-pfrh5-invasion-complex-and-protection-from-plasmodium-falciparum-malaria
#17
Rupert Weaver, Linda Reiling, Gaoqian Feng, Damien R Drew, Ivo Mueller, Peter M Siba, Takafumi Tsuboi, Jack S Richards, Freya J I Fowkes, James G Beeson
Understanding the targets and mechanisms of human immunity to malaria is important for advancing the development of highly efficacious vaccines and serological tools for malaria surveillance. The PfRH5 and PfRipr proteins form a complex on the surface of P. falciparum merozoites that is essential for invasion of erythrocytes and are vaccine candidates. We determined IgG subclass responses to these proteins among malaria-exposed individuals in Papua New Guinea and their association with protection from malaria in a longitudinal cohort of children...
September 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27574520/circulating-micrornas-potential-and-emerging-biomarkers-for-diagnosis-of-human-infectious-diseases
#18
REVIEW
Parmila Verma, Rajan K Pandey, Priyanka Prajapati, Vijay K Prajapati
MicroRNAs (miRNAs) are evolutionary conserved, small non-coding RNA with size ranging from 19 to 24 nucleotides. They endogenously regulate the gene expression at the post transcriptional level either through translation repression or mRNA degradation. MiRNAs have shown the potential to be used as a biomarker for the diagnosis, prognosis, and therapy of infectious diseases. Many miRNAs have shown significantly altered expression during infection. The altered expression of miRNA level in an infected human can be identified by the use of advanced diagnostic tools...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27563669/relationship-between-antibody-levels-igg-binding-to-plasmodium-falciparum-infected-erythrocytes-and-disease-outcome-in-hospitalized-urban-malaria-patients-from-dakar-s%C3%A3-n%C3%A3-gal
#19
Babacar Mbengue, Mouhamadou Mansour Fall, Maguette Sylla Niang, Birahim Niang, Marie Louise Varela, Antoine Marie Diatta, Moustapha Mbow, Kantome Ndiaye, Rokhaya Ndiaye Diallo, Alioune Dieye, Ronald Perraut
Background. Management of clinical malaria requires the development of reliable diagnostic methods and efficient biomarkers for follow-up of patients. Protection is partly based on IgG responses to parasite antigens exposed at the surface of infected erythrocytes (iRBCs). These IgG responses appeared low during clinical infection, particularly in severe disease. Methods. We analyzed the IgG binding capacity to the surface of live erythrocytes infected by knob positive FCR3 strain. Sera from 69 cerebral malaria (CM) and 72 mild malaria (MM) cases were analyzed by ELISA for IgG responses to five antigens from iRBC and by flow cytometry for IgG binding as expressed in labeling index ratio (LIR)...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27546068/minimising-invasiveness-in-diagnostics-developing-a-rapid-urine-based-monoclonal-antibody-dipstick-test-for-malaria
#20
Uri S Markakpo, Kwabena M Bosompem, Mawuli Dzodzomenyo, Anthony Danso-Appiah, Edward E Essuman, William K Anyan, Mitsuko Suzuki, Judith K Stephens, Isaac Anim-Baidoo, Richard H Asmah, Michael F Ofori, Parnor Madjitey, Jonas B Danquah, Naa Adjeley Frempong, Kofi D Kwofie, Michael Amoa-Bosompem, David Sullivan, Julius N Fobil, Isabella A Quakyi
OBJECTIVE: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. METHODS: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. RESULTS: Ninety-six MAb clones were generated, of which 68...
October 2016: Tropical Medicine & International Health: TM & IH
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