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https://www.readbyqxmd.com/read/29032164/a-portable-microfluidic-aptamer-tethered-enzyme-capture-aptec-biosensor-for-malaria-diagnosis
#1
Lewis A Fraser, Andrew B Kinghorn, Roderick M Dirkzwager, Shaolin Liang, Yee-Wai Cheung, Bryce Lim, Simon Chi-Chin Shiu, Marco S L Tang, Dean Andrew, Joseph Manitta, Jack S Richards, Julian A Tanner
There is a critical need for better biosensors for the detection and diagnosis of malaria. We previously developed a DNA aptamer that recognises the Plasmodium falciparum lactate dehydrogenase (PfLDH) enzyme with high sensitivity and specificity. The aptamer was integrated into an Aptamer-Tethered Enzyme Capture (APTEC) assay as a laboratory-based diagnostic approach. However, a portable equipment-free point-of-care aptamer-mediated biosensor could have a significant impact on malaria diagnosis in endemic regions...
October 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28982133/simultaneous-assessment-of-iodine-iron-vitamin-a-malarial-antigenemia-and-inflammation-status-biomarkers-via-a-multiplex-immunoassay-method-on-a-population-of-pregnant-women-from-niger
#2
Eleanor Brindle, Lorraine Lillis, Rebecca Barney, Sonja Y Hess, K Ryan Wessells, Césaire T Ouédraogo, Sara Stinca, Michael Kalnoky, Roger Peck, Abby Tyler, Christopher Lyman, David S Boyle
Deficiencies of vitamin A, iron, and iodine are major public health concerns in many low- and middle-income countries, but information on their status in populations is often lacking due to high costs and logistical challenges associated with assessing micronutrient status. Accurate, user-friendly, and low-cost analytical tools are needed to allow large-scale population surveys on micronutrient status. We present the expansion of a 7-plex protein microarray tool for the simultaneous measurement of up to seven biomarkers with relevance to the assessment of the key micronutrients iron, iodine, and vitamin A, and inflammation and malaria biomarkers: α-1-acid glycoprotein, C-reactive protein, ferritin, retinol binding protein 4, soluble transferrin receptor, thyroglobulin, and histidine-rich protein II...
2017: PloS One
https://www.readbyqxmd.com/read/28969365/evidence-for-potential-underestimation-of-clinical-folate-deficiency-in-resource-limited-countries-using-blood-tests
#3
Asok C Antony
Although a low serum folate concentration is a useful biomarker of pure folate deficiency, the presence of vitamin B12 deficiency or hemolysis or both in individuals with low folate status predictably raises serum folate levels. Therefore, in resource-limited settings where dietary folate deficiency can coexist with vitamin B12 deficiency or malaria or both, the serum folate concentration can range from normal to high, leading to serious underestimation of tissue folate status. This review traces the genesis of an inappropriate overreliance on the serum folate concentration to rule out folate deficiency in vulnerable populations of women and children...
August 1, 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/28968664/a-novel-gametocyte-biomarker-for-superior-molecular-detection-of-the-plasmodium-falciparum-infectious-reservoirs
#4
Edward Essuman, Bryan Grabias, Nitin Verma, Joanna K Chorazeczewski, Abhai K Tripathi, Godfree Mlambo, Ebenezer A Addison, Albert G B Amoah, Isabella Quakyi, Miranda S Oakley, Sanjai Kumar
Background: Complete malaria eradication and optimal use of transmission-reducing interventions requires knowledge of submicroscopic infectious reservoirs among asymptomatic individuals. Even sub-microscopic levels of Plasmodium falciparum gametocytes can infect mosquitoes and promote onward transmission. Most efforts to identify gametocyte carriers utilize PCR amplification of the gametocyte-specific transcript Pfs25. Methods: To expand the repertoire of biomarkers available for superior gametocyte detection, we compared the gene expression profiles of gametocytes versus asynchronous blood stage P...
August 24, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28965490/study-roadmap-for-high-throughput-development-of-easy-to-use-and-affordable-biomarkers-as-diagnostics-for-tropical-diseases-a-focus-on-malaria-and-schistosomiasis
#5
Kokouvi Kassegne, Ting Zhang, Shen-Bo Chen, Bin Xu, Zhi-Sheng Dang, Wang-Ping Deng, Eniola Michael Abe, Hai-Mo Shen, Wei Hu, Takele Geressu Guyo, Solomon Nwaka, Jun-Hu Chen, Xiao-Nong Zhou
BACKGROUND: Interventions are currently being used against 'infectious diseases of poverty', which remain highly debilitating and deadly in most endemic countries, especially malaria, schistosomiasis, echinococcosis and African sleeping sickness. However, major limitations of current 'traditional' methods for diagnosis are neither simple nor convenient for population surveillance, and showed low sensitivity and specificity. Access to novel technologies for the development of adequate and reliable tools are expressly needed...
October 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28946883/igg-antibodies-to-synthetic-gpi-are-biomarkers-of-immune-status-to-both-plasmodium-falciparum-and-plasmodium-vivax-malaria-in-young-children
#6
Camila T França, Connie S N Li Wai Suen, Amandine Carmagnac, Enmoore Lin, Benson Kiniboro, Peter Siba, Louis Schofield, Ivo Mueller
BACKGROUND: Further reduction in malaria prevalence and its eventual elimination would be greatly facilitated by the development of biomarkers of exposure and/or acquired immunity to malaria, as well as the deployment of effective vaccines against Plasmodium falciparum and Plasmodium vivax. A better understanding of the acquisition of immunity in naturally-exposed populations is essential for the identification of antigens useful as biomarkers, as well as to inform rational vaccine development...
September 25, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28934434/high-sensitivity-assays-for-plasmodium-falciparum-infection-by-immuno-polymerase-chain-reaction-detection-of-pfideh-and-pfldh-antigens
#7
Jianbing Mu, John F Andersen, Jesus G Valenzuela, Thomas E Wellems
Background: Rapid diagnostic tests based on Plasmodium falciparum histidine-rich protein II (PfHRP-II) and P. falciparum lactate dehydrogenase (PfLDH) antigens are widely deployed for detection of P. falciparum infection; however, these tests often miss cases of low-level parasitemia, and PfHRP-II tests can give false-negative results when P. falciparum strains do not express this antigen. Methods: We screened proteomic data for highly expressed P. falciparum proteins and compared their features to those of PfHRP-II and PfLDH biomarkers...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28886714/the-oxylipin-and-endocannabidome-responses-in-acute-phase-plasmodium-falciparum-malaria-in-children
#8
Izabella Surowiec, Sandra Gouveia-Figueira, Judy Orikiiriza, Elisabeth Lindquist, Mari Bonde, Jimmy Magambo, Charles Muhinda, Sven Bergström, Johan Normark, Johan Trygg
BACKGROUND: Oxylipins and endocannabinoids are low molecular weight bioactive lipids that are crucial for initiation and resolution of inflammation during microbial infections. Metabolic complications in malaria are recognized contributors to severe and fatal malaria, but the impact of malaria infection on the production of small lipid derived signalling molecules is unknown. Knowledge of immunoregulatory patterns of these molecules in malaria is of great value for better understanding of the disease and improvement of treatment regimes, since the action of these classes of molecules is directly connected to the inflammatory response of the organism...
September 8, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28853859/metal-affinity-enabled-capture-and-release-antibody-reagents-generate-a-multiplex-biomarker-enrichment-system-that-improves-detection-limits-of-rapid-diagnostic-tests
#9
Westley S Bauer, Christopher P Gulka, Lidalee Silva-Baucage, Nicholas M Adams, Frederick R Haselton, David W Wright
Multi-antigen rapid diagnostic tests (RDTs) are highly informative, simple, mobile, and inexpensive, making them valuable point-of-care (POC) diagnostic tools. However, these RDTs suffer from several technical limitations-the most significant being the failure to detect low levels of infection. To overcome this, we have developed a magnetic bead-based multiplex biomarker enrichment strategy that combines metal affinity and immunospecific capture to purify and enrich multiple target biomarkers. Modifying antibodies to contain histidine-rich peptides enables reversible loading onto immobilized metal affinity magnetic beads, generating a novel class of antibodies coined "Capture and Release" (CaR) antibody reagents...
September 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28841253/identification-of-highly-expressed-plasmodium-vivax-proteins-from-clinical-isolates-using-proteomics
#10
Apoorva Venkatesh, Anwesha Lahiri, Panga Jaipal Reddy, Jayanthi Shastri, Sheetal Bankar, Swati Patankar, Sanjeeva Srivastava
PURPOSE: Plasmodium vivax is the most geographically widespread species responsible for malaria in humans. Our study focused on identifying highly expressed parasite proteins using a shotgun proteomics approach. EXPERIMENTAL DESIGN: Parasites (P. vivax) were isolated from 7 patient samples using saponin lysis. Protein extracts from these parasites were processed and subjected to LC-MS/MS analysis. RESULTS: An overall proteome coverage of 586 P...
August 25, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28835253/plasmodium-falciparum-hrp2-elisa-for-analysis-of-dried-blood-spot-samples-in-rural-zambia
#11
Lauren E Gibson, Christine F Markwalter, Danielle W Kimmel, Lwiindi Mudenda, Saidon Mbambara, Philip E Thuma, David W Wright
BACKGROUND: Dried blood spots are commonly used for sample collection in clinical and non-clinical settings. This method is simple, and biomolecules in the samples remain stable for months at room temperature. In the field, blood samples for the study and diagnosis of malaria are often collected on dried blood spot cards, so development of a biomarker extraction and analysis method is needed. METHODS: A simple extraction procedure for the malarial biomarker Plasmodium falciparum histidine-rich protein 2 (HRP2) from dried blood spots was optimized to achieve maximum extraction efficiency...
August 23, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28813452/plasma-metabolomics-reveals-membrane-lipids-aspartate-asparagine-and-nucleotide-metabolism-pathway-differences-associated-with-chloroquine-resistance-in-plasmodium-vivax-malaria
#12
Karan Uppal, Jorge L Salinas, Wuelton M Monteiro, Fernando Val, Regina J Cordy, Ken Liu, Gisely C Melo, Andre M Siqueira, Belisa Magalhaes, Mary R Galinski, Marcus V G Lacerda, Dean P Jones
BACKGROUND: Chloroquine (CQ) is the main anti-schizontocidal drug used in the treatment of uncomplicated malaria caused by Plasmodium vivax. Chloroquine resistant P. vivax (PvCR) malaria in the Western Pacific region, Asia and in the Americas indicates a need for biomarkers of resistance to improve therapy and enhance understanding of the mechanisms associated with PvCR. In this study, we compared plasma metabolic profiles of P. vivax malaria patients with PvCR and chloroquine sensitive parasites before treatment to identify potential molecular markers of chloroquine resistance...
2017: PloS One
https://www.readbyqxmd.com/read/28807026/circulating-micrornas-in-malaria-infection-bench-to-bedside
#13
REVIEW
Supat Chamnanchanunt, Suthat Fucharoen, Tsukuru Umemura
Severe malaria has a poor prognosis with a morbidity rate of 80% in tropical areas. The early parasite detection is one of the effective means to prevent severe malaria of which specific treatment strategies are limited. Many clinical characteristics and laboratory testings have been used for the early diagnosis and prediction of severe disease. However, a few of these factors could be applied to clinical practice. MicroRNAs (miRNAs) were demonstrated as useful biomarkers in many diseases such as malignant diseases and cardiovascular diseases...
August 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28768839/serum-soluble-transferrin-receptor-concentrations-are-elevated-in-congolese-children-with-glucose-6-phosphate-dehydrogenase-variants-but-not-sickle-cell-variants-or-%C3%AE-thalassemia
#14
Mikaela K Barker, Amanda M Henderson, Karimah Naguib, Suzanne M Vercauteren, Angela M Devlin, Arianne Y Albert, Esto Bahizire, Pierrot L Tugirimana, Pierre Z Akilimali, Erick Boy, Tim J Green, Crystal D Karakochuk
Background: Anemia is common in Congolese children, and inherited blood disorders may be a contributing cause. The presence of sickle cell variants, X-linked glucose-6-phosphate dehydrogenase (G6PD) deficiency and α-thalassemia, has been previously reported. G6PD A- deficiency is characterized by the co-inheritance of G6PD 376 and 202 variants and is common in sub-Saharan Africa.Objective: We aimed to measure the associations between inherited blood disorders and hemoglobin, ferritin, and soluble transferrin receptor (sTfR) concentrations in Congolese children...
September 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28753985/new-biomarkers-and-diagnostic-tools-for-the-management-of-fever-in-low-and-middle-income-countries-an-overview-of-the-challenges
#15
REVIEW
Camille Escadafal, Christian Nsanzabana, Julie Archer, Violet Chihota, William Rodriguez, Sabine Dittrich
A lack of simple, inexpensive, and rapid diagnostic tests for febrile illnesses other than malaria leads to overtreatment with antibiotics for those who test negative for malaria, and contributes to the global rise in antimicrobial resistance. New tests for the detection of host biomarkers provide promising tools to differentiate bacterial from non-bacterial infections in febrile patients. However, most available biomarker tests are not currently used in resource-limited settings, and very few evaluations have been performed in low- and middle-income country populations with non-severe febrile illness...
July 21, 2017: Diagnostics
https://www.readbyqxmd.com/read/28718841/development-of-an-immunosensor-for-pfhrp-2-as-a-biomarker-for-malaria-detection
#16
Aver Hemben, Jon Ashley, Ibtisam E Tothill
Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent's suitability for the sensor's development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis...
July 18, 2017: Biosensors
https://www.readbyqxmd.com/read/28711027/high-throughput-and-label-free-parasitemia-quantification-and-stage-differentiation-for-malaria-infected-red-blood-cells
#17
Xiaonan Yang, Zhuofa Chen, Jun Miao, Liwang Cui, Weihua Guan
This work reports a high throughput and label-free microfluidic cell deformability sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). The sensor relies on differentiating the RBC deformability (a mechanical biomarker) that is highly correlated with the infection status. The cell deformability is measured by evaluating the transit time when each individual RBC squeezes through a microscale constriction (cross-section ~5µm×5µm)...
December 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28708072/iron-fortified-complementary-foods-containing-a-mixture-of-sodium-iron-edta-with-either-ferrous-fumarate-or-ferric-pyrophosphate-reduce-iron-deficiency-anemia-in-12-to-36-month-old-children-in-a-malaria-endemic-setting-a-secondary-analysis-of-a-cluster-randomized
#18
Dominik Glinz, Rita Wegmüller, Mamadou Ouattara, Victorine G Diakité, Grant J Aaron, Lorenz Hofer, Michael B Zimmermann, Lukas G Adiossan, Jürg Utzinger, Eliézer K N'Goran, Richard F Hurrell
Iron deficiency anemia (IDA) is a major public health problem in sub-Saharan Africa. The efficacy of iron fortification against IDA is uncertain in malaria-endemic settings. The objective of this study was to evaluate the efficacy of a complementary food (CF) fortified with sodium iron EDTA (NaFeEDTA) plus either ferrous fumarate (FeFum) or ferric pyrophosphate (FePP) to combat IDA in preschool-age children in a highly malaria endemic region. This is a secondary analysis of a nine-month cluster-randomized controlled trial conducted in south-central Côte d'Ivoire...
July 14, 2017: Nutrients
https://www.readbyqxmd.com/read/28656241/hydrogen-sulfide-protects-against-the-development-of-experimental-cerebral-malaria-in-a-c57bl-6-mouse-model
#19
Ping Jiang, Zhishen Xu, Baiquan Xiao, Zhong Han, Jiehong Huang, Jianbang Xu, Zhaorong Lun, Wenliang Zhou
Hydrogen sulfide (H2S) has anti‑inflammatory and neuroprotective properties, particularly during pathological processes. Experimental cerebral malaria (ECM), which is caused by vascular leakage into the brain, is characterized by inflammation, neurological deficits and cerebral hemorrhage. The present study investigated the correlation between ECM genesis and the levels of H2S. The results indicated that the levels of H2S derived from the brain decreased over time following ECM infection, and that the low H2S bioavailability was partially caused by decreased expression of the H2S generating enzyme, cystathionine‑β‑synthase...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28652885/rapid-concentration-and-elution-of-malarial-antigen-histidine-rich-protein-ii-using-solid-phase-zn-ii-resin-in-a-simple-flow-through-pipette-tip-format
#20
Westley S Bauer, Kelly A Richardson, Nicholas M Adams, Keersten M Ricks, David J Gasperino, Simon J Ghionea, Mathew Rosen, Kevin P Nichols, Bernhard H Weigl, Frederick R Haselton, David W Wright
Rapid diagnostic tests (RDTs) designed to function at the point of care are becoming more prevalent in malaria diagnostics because of their low cost and simplicity. While many of these tests function effectively with high parasite density samples, their poor sensitivity can often lead to misdiagnosis when parasitemia falls below 100 parasites/μl. In this study, a flow-through pipette-based column was explored as a cost-effective means to capture and elute more Plasmodium falciparum histidine-rich protein II (HRPII) antigen, concentrating the biomarker available in large-volume lysed whole blood samples into volumes compatible with Plasmodium falciparum-specific RDTs...
May 2017: Biomicrofluidics
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