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Tat peptide

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https://www.readbyqxmd.com/read/29442899/highly-efficient-glioma-targeting-of-tat-peptide-tta1-aptamer-polyephylene-glycol-modified-gelatin-siloxane-nanoparticles
#1
Xiao-Ning Lin, Xinli Tian, Wang Li, Jin Sun, Feng Wei, Wei Feng, Zhi-Chun Huang, Xin-Hua Tian
Gliomas are the most common type of intracranial malignant tumor; however, current treatment approaches are often ineffective due to limited penetration of genes or drugs through the blood-brain barrier (BBB). Here we describe the synthesis of gelatin-siloxane nanoparticles (GS NPs) as candidate gene carriers through a two-step sol-gel process. To increase the efficiency of glioma targeting, human immunodeficiency virus-derived Tat, tumor-targeting aptamer (TTA)1, and polyethylene glycol (PEG) were conjugated to the GS NPs to generate Tat-TTA1-PEG-GS NPs...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29439726/a-novel-cav-derived-cell-penetrating-peptide-efficiently-delivers-exogenous-molecules-through-caveolae-mediated-endocytosis
#2
Gaowei Hu, Wenlv Zheng, Ao Li, Yaru Mu, Mingyu Shi, Tuofan Li, Haitao Zou, Hongxia Shao, Aijian Qin, Jianqiang Ye
Cell-penetrating peptide (CPP) is a promising cargo for delivering bioactive molecules. In this study, the N terminus of VP1 from chicken anemia virus, designated as CVP1, was found to carry enriched arginine residues with α-helix. By confocal imaging, flow cytometry and MTT assay, we identified CVP1 as a novel, safe and efficient CPP. CVP1-FITC peptide could entry different types of cells tested with dose dependence, but without cytotoxic effects. Compared with TAT-FITC peptide, the CVP1-FITC peptide showed much higher cell-penetrating activity...
February 13, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29416029/neuroglobin-boosts-axon-regeneration-during-ischemic-reperfusion-via-p38-binding-and-activation-depending-on-oxygen-signal
#3
Xin Xin Xiong, Feng Pan, Ruo Qiao Chen, Dian Xing Hu, Xin Yao Qiu, Chun Yang Li, Xiao Qiang Xie, Bo Tian, Xiao Qian Chen
Cerebral ischemia causes severe cell death or injury including axon breakdown or retraction in the brain. Axon regeneration is crucial for the functional recovery of injured neurons or brains after ischemia/reperfusion (I/R); however, this process has been proved extremely difficult in adult brains and there is still no effective therapy for it. Here we reported that neuroglobin (Ngb), a novel oxygen-binding or sensor protein existing predominantly in neurons or brains, functions as a driving factor for axon regeneration during I/R...
February 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29406549/enzyme-sensitive-cytotoxic-peptide-dendrimer-conjugates-enhance-cell-apoptosis-and-deep-tumor-penetration
#4
Fu-Hua Liu, Chun-Yuan Hou, Di Zhang, Wen-Jing Zhao, Yong Cong, Zhong-Yu Duan, Zeng-Ying Qiao, Hao Wang
Peptide nanodrugs have been developed as promising antitumor chemotherapeutics because they partially overcome the drawbacks of free peptide drugs, but insufficient tumor penetration and interference of peptide function limit their further application. In this work, we have developed multifunctional peptide conjugated dendrimers for improving tumor penetration, cancer cell-specific peptide delivery and anticancer ability. The cytotoxic peptide KLAK, cell-penetrating peptide TAT and matrix metalloproteinase 2 (MMP2)-sensitive peptide-poly(ethylene glycol) (PEG) were conjugated onto dendrimers by one-pot synthesis to gain PKT-S-PEG...
February 6, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29397453/synergistic-effect-of-the-pro-apoptosis-peptide-kla-tat-and-the-cationic-anticancer-peptide-hprp-a1
#5
Cuihua Hu, Xiaolong Chen, Yibing Huang, Yuxin Chen
In this study, a peptide-peptide co-administration therapy between hybrid peptide kla-TAT and cationic anticancer peptide HPRP-A1 was designed to increase the anticancer activity of the combination peptides through synergistic effect. kla is a pro-apoptotic peptide which could induce rapid cancer cell apoptosis by disruption the mitochondrial membrane when internalized the cells. To enhance more kla peptides pass through cell membrane, a double improvement strategy was designed by chemically conjugation with cell penetration peptide TAT as well as co-administration with cationic membrane active peptide HPRP-A1, and the double anticancer mechanism of the kla-TAT peptide and HPRP-A1 including membrane disruption and apoptosis induction was verified through in vitro experiments...
February 3, 2018: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/29378828/import-of-tat-conjugated-propionyl-coa-carboxylase-using-models-of-propionic-acidemia
#6
Renata Collard, Tomas Majtan, Insun Park, Jan P Kraus
Propionic acidemia is caused by a deficiency of the enzyme propionyl-CoA carboxylase (PCC) located in the mitochondrial matrix. Cell-penetrating peptides, including TAT, offer a potential to deliver a cargo into the mitochondrion. Here, we investigated the delivery of an α6β6 PCC enzyme into mitochondria using HIV transactivator of transcription (TAT) peptide at several levels: into isolated mitochondria, patient fibroblast cells and a mouse model. Western blotting of mitochondria as well as enzyme activity confirmed the import of a fusion TAT-PCC into mitochondria as well as into patient cells and the mitochondrial localization was confirmed additionally by confocal imaging...
January 29, 2018: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29378210/facilitated-ampar-endocytosis-causally-contributes-to-the-maternal-sleep-deprivation-induced-impairments-of-synaptic-plasticity-and-cognition-in-the-offspring-rats
#7
Yanzhi Yu, Zhilin Huang, Chunfang Dai, Yehong Du, Huili Han, Yu Tian Wang, Zhifang Dong
Maternal sleep deprivation (MSD) has been suggested to be associated with increased frequency of neurodevelopmental disorders in offspring in both humans and animal models. However, the underlying cellular and molecular mechanism is still unclear. We have recently reported that MSD at different stages of pregnancy impairs the emotional and cognitive functions, and suppresses hippocampal CA1 long-term potentiation (LTP) in the offspring rats. Here, we report that the MSD induced LTP impairment at the CA1 hippocampus of the offspring rats is associated with increased long-term depression (LTD) and reduced expression of postsynaptic GluA2-containing α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid receptors (AMPARs)...
January 26, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29371939/elucidating-respective-functions-of-two-domains-bir-and-c-helix-of-human-iap-survivin-for-precise-targeted-regulating-mitotic-cycle-apoptosis-and-autophagy-of-cancer-cells
#8
Fabiao Hu, Daxia Pan, Wenyun Zheng, Ting Yan, Xiujuan He, Fuzheng Ren, Yiming Lu, Xingyuan Ma
Survivin was the smallest member of the IAP family, which was over expressed in many different cancers, and considered to be a promising hot target for cancer therapy, and our previous study demonstrated that multiple dominant negative mutants from full-length survivin could have many complex effects on cancer cells, such as cell cycle, apoptosis, and autophagy. But it was not yet known what role the two main domains played in those functions, which would be very important for the design of targeted anticancer drugs and for the interpretation of their molecular mechanisms...
December 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/29358647/unanticipated-functional-diversity-among-the-tata-type-components-of-the-tat-protein-translocase
#9
Ekaterina Eimer, Wei-Chun Kao, Julia Fröbel, Anne-Sophie Blümmel, Carola Hunte, Matthias Müller
Twin-arginine translocation (Tat) systems transport folded proteins that harbor a conserved arginine pair in their signal peptides. They assemble from hexahelical TatC-type and single-spanning TatA-type proteins. Many Tat systems comprise two functionally diverse, TatA-type proteins, denominated TatA and TatB. Some bacteria in addition express TatE, which thus far has been characterized as a functional surrogate of TatA. For the Tat system of Escherichia coli we demonstrate here that different from TatA but rather like TatB, TatE contacts a Tat signal peptide independently of the proton-motive force and restricts the premature processing of a Tat signal peptide...
January 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29349578/in-situ-tissue-labeling-of-cerebral-amyloid-using-hiv-related-tat-peptide
#10
E Maderna, L Colombo, A Cagnotto, G Di Fede, A Indaco, F Tagliavini, M Salmona, G Giaccone
Delivering peptide-based drugs to the brain is a major challenge because of the existence of the blood-brain barrier (BBB). To overcome this problem, cell-penetrating peptides derived from proteins that are able to cross biological membranes have been used as cell-permeable and brain-penetrant compounds. An example is the transactivator of transcription protein transduction domain (Tat) of the human immunodeficiency virus. The basic domain of Tat is formed of arginine and lysine amino acid residues. Tat has been used as brain-penetrant carrier also in therapies for Alzheimer disease (AD), the most common form of dementia characterized by extracellular cerebral deposits of amyloid made up of Aβ peptide...
January 19, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29337043/membrane-permeable-rab27a-is-a-regulator-of-the-acrosome-reaction-role-of-geranylgeranylation-and-guanine-nucleotides
#11
Matías A Bustos, Ornella Lucchesi, María C Ruete, Claudia N Tomes
The acrosome reaction is the regulated exocytosis of mammalian sperm's single secretory granule, essential for fertilization. It relies on small GTPases, the cAMP binding protein Epac, and the SNARE complex, among other components. Here, we describe a novel tool to investigate Rab27-related signaling pathways: a hybrid recombinant protein consisting of human Rab27A fused to TAT, a cell penetrating peptide. With this tool, we aimed to unravel the connection between Rab3, Rab27 and Rap1 in sperm exocytosis and to deepen our understanding about how isoprenylation and guanine nucleotides influence the behaviour of Rab27 in exocytosis...
January 11, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29335541/modulation-of-uvb-induced-carcinogenesis-by-activation-of-alternative-dna-repair-pathways
#12
Yan Sha, Vladimir Vartanian, Nichole Owen, Stephanie J Mengden Koon, Marcus J Calkins, Courtney S Thompson, Zahra Mirafzali, Sara Mir, Lisa E Goldsmith, Huaping He, Chun Luo, Scott M Brown, Paul W Doetsch, Andy Kaempf, Jeong Y Lim, Amanda K McCullough, R Stephen Lloyd
The molecular basis for ultraviolet (UV) light-induced nonmelanoma and melanoma skin cancers centers on cumulative genomic instability caused by inefficient DNA repair of dipyrimidine photoproducts. Inefficient DNA repair and subsequent translesion replication past these DNA lesions generate distinct molecular signatures of tandem CC to TT and C to T transitions at dipyrimidine sites. Since previous efforts to develop experimental strategies to enhance the repair capacity of basal keratinocytes have been limited, we have engineered the N-terminally truncated form (Δ228) UV endonuclease (UVDE) from Schizosaccharomyces pombe to include a TAT cell-penetrating peptide sequence with or without a nuclear localization signal (NLS): UVDE-TAT and UVDE-NLS-TAT...
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29335353/kchip3-n-terminal-31-50-fragment-mediates-its-association-with-trpv1-and-alleviates-inflammatory-hyperalgesia-in-rats
#13
Na-Xi Tian, Yu Xu, Jin-Yu Yang, Lu Li, Xiao-Hong Sun, Yun Wang, Ying Zhang
Potassium voltage-gated channel interacting protein 3 (KChIP3), also termed downstream regulatory element antagonist modulator (DREAM) and calsenilin, is a multifunctional protein belonging to the neuronal calcium sensor (NCS) family. Recent studies revealed the expression of KChIP3 in dorsal root ganglion (DRG) neurons, suggesting the potential role of KChIP3 in peripheral sensory processing. Herein, we show that KChIP3 colocalizes with transient receptor potential ion channel V1 (TRPV1), a critical molecule involved in peripheral sensitization during inflammatory pain...
February 14, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29332035/an-aspartyl-cathepsin-targeted-pet-agent-application-in-an-alzheimer-s-disease-mouse-model
#14
Jonatan A Snir, Mojmir Suchy, Geron A Bindseil, Michael Kovacs, Blaine A Chronik, Robert H E Hudson, Stephen H Pasternak, Robert Bartha
BACKGROUND: Early detection of Alzheimer's disease (AD) pathology is a serious challenge for both diagnosis and clinical trials. The aspartyl protease, Cathepsin D (CatD), is overexpressed in AD and could be a biomarker of disease. We have previously designed a unique contrast agent (CA) for dual-optical and magnetic resonance imaging of the activity of the CatD class of enzymes. OBJECTIVE: To compare the uptake and retention of a novel, more sensitive, and clinically-translatable 68Ga PET tracer targeting CatD activity in 5XFAD mice and non-Tg littermates...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29319606/disruption-of-nnos-nos1ap-protein-protein-interactions-suppresses-neuropathic-pain-in-mice
#15
Wan-Hung Lee, Li-Li Li, Aarti Chawla, Andy Hudmon, Yvonne Y Lai, Michael J Courtney, Andrea G Hohmann
Elevated N-methyl-D-aspartate receptor (NMDAR) activity is linked to central sensitization and chronic pain. However, NMDAR antagonists display limited therapeutic potential due to their adverse side effects. Novel approaches targeting the NR2B-PSD95-nNOS complex to disrupt signaling pathways downstream of NMDARs show efficacy in preclinical pain models. Here, we evaluated the involvement of interactions between neuronal nitric oxide synthase (nNOS) and the nitric oxide synthase 1 adaptor protein (NOS1AP) in pronociceptive signaling and neuropathic pain...
January 9, 2018: Pain
https://www.readbyqxmd.com/read/29317708/connexin-43-hemichannel-as-a-novel-mediator-of-sterile-and-infectious-inflammatory-diseases
#16
Wei Li, Guoqiang Bao, Weiqiang Chen, Xiaoling Qiang, Shu Zhu, Shuaiwei Wang, Mingzhu He, Gaifeng Ma, Mahendar Ochani, Yousef Al-Abed, Huan Yang, Kevin J Tracey, Ping Wang, John D'Angelo, Haichao Wang
Cytoplasmic membrane-bound connexin 43 (Cx43) proteins oligomerize into hexameric channels (hemichannels) that can sometimes dock with hemichannels on adjacent cells to form gap junctional (GJ) channels. However, the possible role of Cx43 hemichannels in sterile and infectious inflammatory diseases has not been adequately defined due to the lack of selective interventions. Here we report that a proinflammatory mediator, the serum amyloid A (SAA), resembled bacterial endotoxin by stimulating macrophages to up-regulate Cx43 expression and double-stranded RNA-activated protein kinase R (PKR) phosphorylation in a TLR4-dependent fashion...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315333/cell-penetrating-peptide-driven-cre-recombination-in-porcine-primary-cells-and-generation-of-marker-free-pigs
#17
Qianqian Kang, Zhaolin Sun, Zhiyuan Zou, Ming Wang, Qiuyan Li, Xiaoxiang Hu, Ning Li
Cell-penetrating peptides (CPPs) have been increasingly used to deliver various molecules, both in vitro and in vivo. However, there are no reports of CPPs being used in porcine fetal fibroblasts (PFFs). The increased use of transgenic pigs for basic research and biomedical applications depends on the availability of technologies for efficient genetic-modification of PFFs. Here, we report that three CPPs (CPP5, TAT, and R9) can efficiently deliver active Cre recombinase protein into PFFs via an energy-dependent endocytosis pathway...
2018: PloS One
https://www.readbyqxmd.com/read/29314706/superior-hiv-1-tar-binders-with-conformationally-constrained-r52-arginine-mimics-in-tat-48-57-peptide
#18
Govind S Bhosle, Shalmali Kharche, Santosh Kumar, Durba Sengupta, Souvik Maiti, Moneesha Fernandes
We report a hundred-fold increase in binding affinity of the Tat(48-57) peptide to HIV-1 TAR RNA by replacing R52, an essential and critical residue for Tat's specific binding, by (2S,4S)-4-guanidinoproline. The resulting αTat1M peptide is a far superior binder than γTat1M, a peptide containing another conformationally constrained arginine mimic, (2S,4S)-4-amino-N-(3-guanidinopropyl)-proline, or even the control Tat (CtrlTat) itself. Our observations are supported by CD, ITC, gel electrophoresis and UV spectroscopy studies...
January 4, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29303375/structure-optimization-to-improve-the-delivery-efficiency-and-cell-selectivity-of-a-tumor-targeting-cell-penetrating-peptide
#19
Xue-Wei Cao, Xu-Zhong Yang, Xuan Du, Long-Yun Fu, Tao-Zhu Zhang, Han-Wen Shan, Jian Zhao, Fu-Jun Wang
Cell-penetrating peptide (CPP) is used for the delivery of biomacromolecules across the cell membrane and is limited in cancer therapy due to the lack of cell selectivity. Epidermal growth factor receptor (EGFR) has been widely used in clinical targeted therapy for tumors. Here we reported a novel tumor targeting cell-penetrating peptide (TCPP), EHB (ELBD-C6H) with 20-fold and 3000-fold greater transmembrane ability and tumor cell selectivity than our previously reported S3-HBD and classic CPP TAT, respectively...
January 5, 2018: Journal of Drug Targeting
https://www.readbyqxmd.com/read/29299217/a-mini-review-of-tat-myod-fused-proteins-state-of-the-art-and-problems-to-solve
#20
Marco Patruno, Luca Melotti, Chiara Gomiero, Roberta Sacchetto, Ohad Topel, Tiziana Martinello
The transcriptional activator TAT is a small peptide essential for viral replication and possesses the property of entering the cells from the extracellular milieu, acting as a membrane shuttle. In order to safely differentiate cells an innovative methodology, based on the fusion of transcription factors and the TAT sequence, is discussed in this short review. In several studies, it has been demonstrated that TAT protein can be observed in the cell nucleus after few hours from the inoculation although its way of action is not fully understood...
December 5, 2017: European Journal of Translational Myology
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