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Atomic force microscopy

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https://www.readbyqxmd.com/read/28088669/micro-mechanical-properties-of-the-tendon-to-bone-attachment
#1
Alix C Deymier, Yiran An, John J Boyle, Andrea G Schwartz, Victor Birman, Guy M Genin, Stavros Thomopoulos, Asa H Barber
: The tendon-to-bone attachment (enthesis) is a complex hierarchical tissue that connects stiff bone to compliant tendon. The attachment site at the micrometer scale exhibits gradients in mineral content and collagen orientation, which likely act to minimize stress concentrations. The physiological micromechanics of the attachment thus define resultant performance, but difficulties in sample preparation and mechanical testing at this scale have restricted understanding of structure-mechanical function...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28088568/nanostructured-tin-oxide-films-physical-synthesis-characterization-and-gas-sensing-properties
#2
S M Ingole, S T Navale, Y H Navale, D K Bandgar, F J Stadler, R S Mane, N S Ramgir, S K Gupta, D K Aswal, V B Patil
Nanostructured tin oxide (SnO2) films are synthesized using physical method i.e. thermal evaporation and are further characterized with X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, and atomic force microscopy measurement techniques for confirming its structure and morphology. The chemiresistive properties of SnO2 films are studied towards different oxidizing and reducing gases where these films have demonstrated considerable selectivity towards oxidizing nitrogen dioxide (NO2) gas with a maximum response of 403% to 100ppm @200°C, and fast response and recovery times of 4s and 210s, respectively, than other test gases...
January 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28088122/a-comparative-study-on-the-effects-of-ultrathin-luminescent-graphene-oxide-quantum-dot-goqd-and-graphene-oxide-go-nanosheets-on-the-interfacial-interactions-and-mechanical-properties-of-an-epoxy-composite
#3
B Karimi, B Ramezanzadeh
The reinforcement effect of graphene oxide nanosheets on the mechanical properties of an epoxy coating has been extensively studied. However, the effect of graphene oxide quantum dot (GOQD) as a new unique carbon based nanomaterial (with lateral dimension of 5-6nm and thickness of one carbon atom) on the mechanical properties of epoxy coating has not been reported and compared with GO yet. So this study aims at fabrication of a high-performance polymer composite with unique mechanical properties using GOQD nanosheets...
January 5, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28086938/alpha-enolase-eno1-controls-alpha-v-beta-3-integrin-expression-and-regulates-pancreatic-cancer-adhesion-invasion-and-metastasis
#4
Moitza Principe, Simone Borgoni, Mariafrancesca Cascione, Michelle Samuel Chattaragada, Sammy Ferri-Borgogno, Michela Capello, Sara Bulfamante, Jennifer Chapelle, Francesca Di Modugno, Paola Defilippi, Paola Nisticò, Paola Cappello, Chiara Riganti, Stefano Leporatti, Francesco Novelli
BACKGROUND: We have previously shown that in pancreatic ductal adenocarcinoma (PDA) cells, the glycolytic enzyme alpha-enolase (ENO1) also acts as a plasminogen receptor and promotes invasion and metastasis formation. Moreover, ENO1 silencing in PDA cells induces oxidative stress, senescence and profoundly modifies PDA cell metabolism. Although anti-ENO1 antibody inhibits PDA cell migration and invasion, little is known about the role of ENO1 in regulating cell-cell and cell-matrix contacts...
January 13, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28081363/distance-dependent-measurements-of-the-conductance-of-porphyrin-nanorods-studied-with-conductive-probe-atomic-force-microscopy
#5
Xianglin Zhai, Denzel Alexander, Pedro Derosa, Jayne C Garno
Protocols for nanopatterning porphyrins on Au(111) were developed based on immersion particle lithography. Porphyrins with and without a central metal ion, 5,10,15,20-tetraphenyl-21H,23H-porphyrin (TPP) and 5,10,15,20-tetraphenyl-21H,23H-porphyrin cobalt(II) (CoTPP) were selected for study, which spontaneously formed nanorod geometries depending on concentration parameters. The elongated shapes of the nanorods offers an opportunity for successive distance-dependent conductive probe-atomic force microscopy (CP-AFM) measurements along the length of the nanorods...
January 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28080032/heteroprotein-complex-formation-of-bovine-lactoferrin-and-pea-protein-isolate-a-multiscale-structural-analysis
#6
Eda Adal, Amin Sadeghpour, Simon D A Connell, Michael Rappolt, Esra Ibanoglu, Anwesha Sarkar
Associative electrostatic interactions between two oppositely charged globular proteins, lactoferrin (LF) and pea protein isolate (PPI), the latter being a mixture of vicilin, legumin and convicilin, was studied with a specific PPI/ LF molar ratio at room temperature. Structural aspects of the electrostatic complexes probed at different length scales were investigated as a function of pH by means of different complementary techniques, namely with dynamic light scattering, small angle X-ray scattering (SAXS), turbidity measurements and atomic force microscopy (AFM)...
January 12, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28080029/one-step-fabrication-of-3d-nano-hierarchical-ni-nanomace-array-to-sinter-with-ag-nps-and-the-interfacial-analysis
#7
Wei Zhou, Zhen Zheng, Chunqing Wang, Zhongtao Wang, Rong An
Three-dimensional (3D) nano-hierarchical Ni nanomace (Ni NM) array was fabricated on copper substrate by only one step with electroplating method, the unique structure was covered with Au film (Ni/Au NM) without changing its morphology and in the following step, it was sintered with silver nanoparticle (Ag NP) paste. The structure of Ni NM array and its surface morphology were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). The sintered interface was investigated by SEM, Transmission Electron Microscopy (TEM) and Energy-Dispersive X-Ray Spectroscopy (EDS) to analyze the sintering mechanism...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28080023/microbial-nanoscopy-breakthroughs-challenges-and-opportunities
#8
Yves F Dufrêne
Studying the structure, properties, and interactions of microbial cells is key to understanding the functions of the microbiome. Recent advances in nanotechnology have offered new tools to probe microbes at the single-molecule and single-cell levels. In this issue of ACS Nano, Kumar et al. present an atomic force microscopy method that is capable of imaging the nanoscale organization of bacterial proteins in native, curved membranes. This study represents an important step forward in the development of nanoscopy techniques for analyzing biological systems with large curvature and vertical dimensions, such as membrane vesicles and bacterial cells...
January 12, 2017: ACS Nano
https://www.readbyqxmd.com/read/28078888/low-temperature-wet-conformal-nickel-silicide-deposition-for-transistor-technology-through-an-organometallic-approach
#9
Tsung-Han Lin, Tigran Margossian, Michele De Marchi, Maxime Thammasack, Dmitry Y Zemlyanov, Sudhir Kumar, Jakub Jagielski, Li-Qing Zheng, Chih-Jen Shih, Renato Zenobi, Giovanni De Micheli, David Baudouin, Pierre-Emmanuel Gaillardon, Christophe Copéret
The race for performant integrated circuits is nowadays facing a downscale limitation. To overpass this nanoscale limit, modern transistors with complex geometries have flourished allowing higher performance and energy efficiency. Accompany with this breakthrough, challenges toward high-performance devices have emerged on each key preparation step, such as the inhomogeneous coverage issue and thermal-induced short circuit issue of metal silicide formation. In this respect, we developed a two-step organometallic approach for nickel silicide formation under near-ambient temperature...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28078590/quantifying-cd95-cl-cd95l-implications-in-cell-mechanics-and-membrane-tension-by-atomic-force-microscopy-based-force-measurements
#10
Anaïs Sadoun, Pierre-Henri Puech
Atomic force microscopy (AFM) is an invaluable tool to investigate the structure of biological material surfaces by imaging them at nanometer scale in physiological conditions. It can also be used to measure the forces and mechanics from single molecule interaction to cell-cell adhesion. Here, we present a methodology that allows to quantify cell elastic properties (using the Young modulus) and cell membrane tension modulated by CD95/cl-CD95L interactions by coupling nanoindentation and membrane tube pulling using suitably decorated AFM levers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28078419/nanostructured-nio-based-reagentless-biosensor-for-total-cholesterol-and-low-density-lipoprotein-detection
#11
Gurpreet Kaur, Monika Tomar, Vinay Gupta
Nanostructured nickel oxide (NiO) thin film has been explored as a matrix to develop a reagentless biosensor for free and total cholesterol as well as low density lipoprotein (LDL) detection. The redox property of the matrix has been exploited to enhance the electron transfer between the enzyme and the electrode as well as to eliminate the toxic mediator in solution. X-ray diffraction, scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy were carried out to characterize the NiO thin film...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28075590/nanometer-sized-water-bridge-and-pull-off-force-in-afm-at-different-relative-humidities-reproducibility-measurement-and-model-based-on-surface-tension-change
#12
Miroslav Bartošík, Lukáš Kormoš, Lukáš Flajšman, Radek Kalousek, Jindřich Mach, Zuzana Lišková, David Nezval, Vojtěch Švarc, Tomáš Šamořil, Tomáš Šikola
This article deals with the analysis of the relationship between the pull-off force measured by atomic force microscopy and the dimensions of water bridge condensed between a hydrophilic silicon oxide tip and a silicon oxide surface under ambient conditions. Our experiments have shown that the pull-off force increases linearly with the radius of the tip and nonmonotonically with the relative humidity (RH). The latter dependence generally consists of an initial constant part changing to a convex-concave-like increase of the pull-off force and finally followed by a concave-like decrease of this force...
January 11, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28075577/reversible-unfolding-and-folding-of-the-metalloprotein-ferredoxin-revealed-by-single-molecule-atomic-force-microscopy
#13
Hai Lei, Yabin Guo, Xiaodong Hu, Chunguang Hu, Xiaotang Hu, Hongbin Li
Plant type [2Fe-2S] ferredoxins function primarily as electron transfer proteins in photosynthesis. Studying the unfolding-folding of ferredoxins in vitro is challenging, since the unfolding of ferredoxin is often irreversible due to the loss or disintegration of the iron-sulfur cluster. Additionally, the in vivo folding of holo-ferredoxin requires ferredoxin biogenesis proteins. Here we employed atomic force microscopy-based single molecule force microscopy and protein engineering techniques to directly study the mechanical unfolding and refolding of a plant type [2Fe-2S] ferredoxin from cyanobacteria Anabaena...
January 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28075549/solvent-morphology-property-relationship-of-ptb7-pc71bm-polymer-solar-cells
#14
Shuai Guo, Weijia Wang, Eva M Herzig, Anna Naumann, Gregory Tainter, Jan Perlich, Peter Muller-Buschbaum
The influence of three different solvents and a solvent additive on the morphology and photovoltaic performance of bulk heterojunction films made of the copolymer based on thieno[3,4-b]thiophene-alt-benzodithiophene unit PTB7-F40 blended with [6,6]-phenyl-C71-butyric acid methyl ester (PC71BM) is investigated. Optical microscopy and atomic force microscopy (AFM) are combined with X-ray reflectivity (XRR) and grazing incidence small and wide angle X-ray scattering (GISAXS and GIWAXS) enabling the characterization of the morphology of the whole photoactive film...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28073120/nano-pelargonidin-protects-hyperglycemic-induced-l6-cells-against-mitochondrial-dysfunction
#15
Asmita Samadder, Debojyoti Tarafdar, Suresh K Abraham, Kumaresh Ghosh, Anisur Rahman Khuda-Bukhsh
Nano-encapsulation of several natural products has become an important tool in enhancing the bioavailability of some modern drugs against many diseases. Pelargonidin is an anthocyanidin found in many fruits and vegetables. Pelargonidin is loaded with poly-lactide-co-glycolic-acid, a non-toxic biodegradable polymer, to produce nano-pelargonidin. Size, morphology, zeta potential, and planar uniformity of formulated nano-pelargonidin were determined by atomic force microscopy and dynamic light scattering. The time required for cellular entry, folds of nano-pelargonidin, and drug encapsulation efficiency of poly-lactide-co-glycolic-acid were also ascertained...
January 10, 2017: Planta Medica
https://www.readbyqxmd.com/read/28071732/homoepitaxial-growth-of-metal-halide-crystals-investigated-by-reflection-high-energy-electron-diffraction
#16
Pei Chen, Padmanaban S Kuttipillai, Lili Wang, Richard R Lunt
We report the homoepitaxial growth of a metal halide on single crystals investigated with in situ reflection high-energy electron diffraction (RHEED) and ex situ atomic force microscopy (AFM). Epitaxial growth of NaCl on NaCl (001) is explored as a function of temperature and growth rate which provides the first detailed report of RHEED oscillations for metal halide growth. Layer-by-layer growth is observed at room temperature accompanied by clear RHEED oscillations while the growth mode transitions to an island (3D) mode at low temperature...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069495/surface-modification-of-copolymerized-films-from-three-armed-biodegradable-macromers-an-analytical-platform-for-modified-tissue-engineering-scaffolds
#17
Benno M Müller, Rudi Loth, Peter-Georg Hoffmeister, Friederike Zühl, Liv Kalbitzer, Michael C Hacker, Michaela Schulz-Siegmund
: The concept of macromers allows for a broad adjustment of biomaterial properties by macromer chemistry or copolymerization. Copolymerization strategies can also be used to introduce reactive sites for subsequent surface modification. Control over surface features enables adjustment of cellular reactions with regard to site and object of implantation. We designed macromer-derived polymer films which function as non-implantable analytical substrates for the investigation of surface properties of equally composed scaffolds for bone tissue engineering...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28069413/characterization-of-planar-biomimetic-lipid-films-composed-of-phosphatidylethanolamines-and-phosphatidylglycerols-from-escherichia-coli
#18
Dorota Konarzewska, Joanna Juhaniewicz, Alişan Güzeloğlu, Sławomir Sęk
We have characterized planar lipid films composed of phosphatidylethanolamines (PE) and phosphatidylglycerols (PG) from E. coli bacteria. The nature of the interactions and miscibility of PE and PG components within mixed lipid films was evaluated based on surface pressure measurements and Brewster angle microscopy imaging at the air-water interface. We have found that PE and PG components show tendency to form separated domains at surface pressures relevant for biological membranes. Further, we have directly compared mechanisms of formation of supported lipid bilayers either on mica or Au(111) by spreading of small unilamellar vesicles...
January 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28069377/development-of-an-aptamer-ampicillin-conjugate-for-treating-biofilms
#19
Cheng Lijuan, Yan Xing, Wu Minxi, Li Wenkai, Deng Le
Biofilm formation involves the development of extracellular matrix and initially depends on adherence and tropism by flagellar movement. With the widespread development of antibiotic resistance and tolerance of biofilms, there is a growing need for novel anti-infective strategies. No currently approved medications specifically target biofilms. Aptamers are single-stranded nucleic acid molecules that may bind to their targets with high affinity and affect the target functions. We developed a bifunctional conjugate by linking an aptamer targeting bacterial flagella with ampicillin...
January 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28068880/low-intensity-vibrations-normalize-adipogenesis-induced-morphological-and-molecular-changes-of-adult-mesenchymal-stem-cells
#20
Oznur Baskan, Gulistan Mese, Engin Ozcivici
Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily to high-frequency low-intensity mechanical vibrations to understand molecular, morphological and ultrastructural adaptations to mechanical signals during adipogenesis. D1-ORL-UVA mouse bone marrow mesenchymal stem cells were cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration signals (15 min/day, 90 Hz, 0.1 g) were applied to one group of adipogenic cells, while the other adipogenic group served as a sham control...
January 1, 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
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