Chase J Morgan, Eray Enustun, Emily G Armbruster, Erica A Birkholz, Amy Prichard, Taylor Forman, Ann Aindow, Wichanan Wannasrichan, Sela Peters, Koe Inlow, Isabelle L Shepherd, Alma Razavilar, Vorrapon Chaikeeratisak, Benjamin A Adler, Brady F Cress, Jennifer A Doudna, Kit Pogliano, Elizabeth Villa, Kevin D Corbett, Joe Pogliano
UNLABELLED: Targeting proteins to specific subcellular destinations is essential in prokaryotes, eukaryotes, and the viruses that infect them. Chimalliviridae phages encapsulate their genomes in a nucleus-like replication compartment composed of the protein chimallin (ChmA) that excludes ribosomes and decouples transcription from translation. These phages selectively partition proteins between the phage nucleus and the bacterial cytoplasm. Currently, the genes and signals that govern selective protein import into the phage nucleus are unknown...
March 21, 2024: bioRxiv