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https://www.readbyqxmd.com/read/28745078/hair-cortisol-in-relation-to-acute-and-post-traumatic-stress-symptoms-in-children-and-adolescents
#1
Joana Straub, Lena Marie Klaubert, Susann Schmiedgen, Clemens Kirschbaum, Lutz Goldbeck
BACKGROUND: We report on the preliminary results of two independent studies that (1) compare the hair cortisol concentrations (HCC) of healthy controls with patients displaying post-traumatic stress symptoms (PTSS, study 1+2), (2) investigate whether pre-trauma HCC are predictive for the development of acute stress symptoms (ASS) and PTSS (study 1) and (3) determine whether HCC correlate with PTSS in a clinical sample of children (study 2). METHODS: In study 1, the clinical symptoms of 35 minors were examined one (T1) and seven weeks (T2) after surgery following an accident...
July 26, 2017: Anxiety, Stress, and Coping
https://www.readbyqxmd.com/read/28743979/expression-of-rmrp-rna-is-regulated-in-chondrocyte-hypertrophy-and-determines-chondrogenic-differentiation
#2
Mandy M F Steinbusch, Marjolein M J Caron, Don A M Surtel, Franziska Friedrich, Ekkehart Lausch, Ger J M Pruijn, Wouter Verhesen, Blanche L M Schroen, Lodewijk W van Rhijn, Bernhard Zabel, Tim J M Welting
Mutations in the RMRP-gene, encoding the lncRNA component of the RNase MRP complex, are the origin of cartilage-hair hypoplasia. Cartilage-hair hypoplasia is associated with severe dwarfism caused by impaired skeletal development. However, it is not clear why mutations in RMRP RNA lead to skeletal dysplasia. Since chondrogenic differentiation of the growth plate is required for development of long bones, we hypothesized that RMRP RNA plays a pivotal role in chondrogenic differentiation. Expression of Rmrp RNA and RNase MRP protein subunits was detected in the murine growth plate and during the course of chondrogenic differentiation of ATDC5 cultures, where Rmrp RNA expression was found to be correlated with chondrocyte hypertrophy...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743298/r-spondin2-a-novel-target-of-nobox-identification-of-variants-in-a-cohort-of-women-with-primary-ovarian-insufficiency
#3
Justine Bouilly, Isabelle Beau, Sara Barraud, Valérie Bernard, Brigitte Delemer, Jacques Young, Nadine Binart
BACKGROUND: R-spondin2 (Rspo2) is a secreted agonist of the canonical Wnt/β-catenin signaling pathway. Rspo2 plays a key role in development of limbs, lungs and hair follicles, and more recently during ovarian follicle development. Rspo2 heterozygous deficient female mice become infertile around 4 months of age mimicking primary ovarian insufficiency (POI). The study aimed to investigate the regulation of RSPO2 and its potential involvement in pathophysiology of POI. METHODS: We cloned the RSPO2 promoter and performed transcriptional assays to determine if RSPO2 can be regulated by NOBOX, an ovarian transcription factor...
July 25, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28743240/whole-brain-radiotherapy-versus-stereotactic-radiosurgery-for-4-10-brain-metastases-a-phase-iii-randomised-multicentre-trial
#4
Jaap D Zindler, Anna M E Bruynzeel, Daniëlle B P Eekers, Coen W Hurkmans, Ans Swinnen, Philippe Lambin
BACKGROUND: Maintenance of quality of life is the primary goal during treatment of brain metastases (BM). This is a protocol of an ongoing phase III randomised multicentre study. This study aims to determine the exact additional palliative value of stereotactic radiosurgery (SRS) over whole brain radiotherapy (WBRT) in patients with 4-10 BM. METHODS: The study will include patients with 4-10 BM from solid primary tumours diagnosed on a high-resolution contrast-enhanced MRI scan with a maximum lesional diameter of 2...
July 25, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28739526/chronic-mild-stress-exacerbates-severity-of-experimental-autoimmune-encephalomyelitis-in-association-with-altered-non-coding-rna-and-metabolic-biomarkers
#5
Brietta Gerrard, Vaibhav Singh, Olena Babenko, Isabelle Gauthier, V Wee Yong, Igor Kovalchuk, Artur Luczak, Gerlinde A S Metz
The causal factors determining the onset and severity of multiple sclerosis (MS) are not well understood. Here, we investigated the influence of chronic stress on clinical symptoms, metabolic and epigenetic manifestations of experimental autoimmune encephalomyelitis (EAE), a common animal model of MS. Lewis rats were immunized for monophasic EAE with MBP69-88 and were exposed to chronic stress for 37 days starting 7 days prior to immunization. The exposure to stress accelerated and exacerbated the clinical symptoms of EAE...
July 21, 2017: Neuroscience
https://www.readbyqxmd.com/read/28738902/a-comparative-effectiveness-study-of-two-culturally-competent-models-of-diabetes-self-management-programming-for-latinos-from-low-income-households
#6
Janet Page-Reeves, Lidia Regino, Cristina Murray-Krezan, Molly Bleecker, Erik Erhardt, Mark Burge, Elaine Bearer, Shiraz Mishra
BACKGROUND: Diabetes risk is extremely high for Latinos from low-income households. Health guidelines recommend that individuals learn strategies to self-manage their diabetes, but getting people to adopt required lifestyle changes is challenging and many people are not able to prevent their pre-diabetes from escalating or effectively control their diabetes. Systematic reviews show that culturally competent self-management programs can significantly improve diabetes outcomes and different models for culturally competent programming have been developed...
July 24, 2017: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/28738799/prevalence-of-head-lice-infestation-and-pediculicidal-effect-of-permethrine-shampoo-in-primary-school-girls-in-a-low-income-area-in-southeast-of-iran
#7
Moussa Soleimani-Ahmadi, Seyed Aghil Jaberhashemi, Mehdi Zare, Alireza Sanei-Dehkordi
BACKGROUND: Head lice infestation is a common public health problem that is most prevalent in primary school children throughout the world, especially in developing countries including different parts of Iran. This study aimed to determine the prevalence and risk factors associated with head lice infestation and pediculicidal effect of 1% permethrin shampoo in primary schools girls of Bashagard County, one of the low socioeconomic areas in southeast of Iran. METHODS: In this interventional study six villages with similar demographical situations were selected and randomly assigned into intervention and control areas...
July 24, 2017: BMC Dermatology
https://www.readbyqxmd.com/read/28733235/determination-of-fumonisin-b1-levels-in-body-fluids-and-hair-from-piglets-fed-fumonisin-b1-contaminated-diets
#8
Pollyana C M C Souto, Alessandra V Jager, Fernando G Tonin, Tânia Petta, Mayra C Di Gregório, Anne-Marie Cossalter, Philippe Pinton, Isabelle P Oswald, George E Rottinghaus, Carlos A F Oliveira
The levels of fumonisin B1 (FB1) residues in plasma, urine, feces and hair from 24 piglets fed FB1-contaminated diets containing 3.1, 6.1 or 9.0 μg FB1.g(-1) for 28 days were determined using liquid chromatography coupled to mass spectrometry (LC-MS/MS). The levels of FB1 in plasma, urine, feces and pooled hair (n = 3) samples varied from 0.15-1.08 μg.L(-1), 16.09-75.01 μg.L(-1), 1.87-13.89 μg.g(-1) and 2.08-8.09 ng.g(-1), respectively. Significant correlations (r = 0.808-0.885; P < 0.001; N = 18) were found between FB1 intake and plasma FB1 on days 7, 14, 21 and 28...
July 18, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28731299/human-biomonitoring-of-lead-exposure
#9
Katrin Klotz, Thomas Göen
After a chronic exposure, lead accumulates in the human body, especially in bones and teeth. Critical effects of lead affect the nervous system, reproduction, fertility as well as genotoxicity and carcinogenicity [1]. Analyses of lead concentrations in human biological material are performed using inductively coupled plasma mass spectrometry and atomic absorption spectrometry, but also electrochemical methods and X-ray fluorescence spectroscopy. The predominant sample matrices include blood and bone, as well as urine, hair, nail, and saliva...
April 10, 2017: Metal Ions in Life Sciences
https://www.readbyqxmd.com/read/28730424/possible-immunomodulating-effect-of-retinol-on-cytokines-secretion-in-patients-with-recurrent-furunculosis
#10
Danuta Nowicka, Ewelina Grywalska, Anna Hymos, Michał Mielnik, Jacek Roliński
Recurrent furunculosis is an infection of hair follicles which results in formation of abscesses. Previous studies showed that the pathogenesis of the disease may include an immune-mediated component as the proliferative response of peripheral blood lymphocytes to staphylococcal antigen is depressed. The aim of our study was to evaluate cytokines concentration in the plasma of patients with recurrent furunculosis and to determine whether retinol affects the secretion of those cytokines in patients with recurrent furunculosis and healthy subjects...
July 20, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28725654/stem-cells-from-human-hair-follicles-first-mechanical-isolation-for-immediate-autologous-clinical-use-in-androgenetic-alopecia-and-hair-loss
#11
Pietro Gentile, Maria G Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
BACKGROUND: Hair follicles are known to contain a well-characterized niche for adult stem cells: the bulge, which contains epithelial and melanocytic stem cells. Stem cells in the hair bulge, a clearly demarcated structure within the lower permanent portion of hair follicles, can generate the interfollicular epidermis, hair follicle structures, and sebaceous glands. The bulge epithelial stem cells can also reconstitute in an artificial in vivo system to a new hair follicle. METHODS: In this study, we have developed a new method to isolate human adult stem cells by mechanical centrifugation of punch biopsy from human hair follicles without culture condition...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/28725240/appraisal-of-testicular-volumes-volumes-matching-ultrasound-values-referenced-to-stages-of-genital-development
#12
Juan F Sotos, Naomi J Tokar
BACKGROUND: Testicular volumes obtained with orchidometers or external linear measurements in the scrotum (centimeter ruler or calipers) grossly over-estimate ultrasound volumes, have much variability and may not be accurate or reproducible. The reference of the values obtained by orchidometers or US, to age or Tanner stages is not useful to determine the normal values for stages of puberty, because overlapping of ages and values. Pubertal development is determined by two events, genital and pubic hair development, that should be analyzed independently because one could be out of step with the other...
2017: International Journal of Pediatric Endocrinology
https://www.readbyqxmd.com/read/28725177/characterization-of-lgr5-progenitor-cell-transcriptomes-after-neomycin-injury-in-the-neonatal-mouse-cochlea
#13
Shasha Zhang, Yuan Zhang, Pengfei Yu, Yao Hu, Han Zhou, Lingna Guo, Xiaochen Xu, Xiaocheng Zhu, Muhammad Waqas, Jieyu Qi, Xiaoli Zhang, Yan Liu, Fangyi Chen, Mingliang Tang, Xiaoyun Qian, Haibo Shi, Xia Gao, Renjie Chai
Lgr5+ supporting cells (SCs) are enriched hair cell (HC) progenitors in the cochlea. Both in vitro and in vivo studies have shown that HC injury can spontaneously activate Lgr5+ progenitors to regenerate HCs in the neonatal mouse cochlea. Promoting HC regeneration requires the understanding of the mechanism of HC regeneration, and this requires knowledge of the key genes involved in HC injury-induced self-repair responses that promote the proliferation and differentiation of Lgr5+ progenitors. Here, as expected, we found that neomycin-treated Lgr5+ progenitors (NLPs) had significantly greater HC regeneration ability, and greater but not significant proliferation ability compared to untreated Lgr5+ progenitors (ULPs) in response to neomycin exposure...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28719105/screening-for-hazardous-drinking-in-nursing-home-residents-evaluating-the-validity-of-the-current-cut-offs-of-the-alcohol-use-disorder-identification-test-consumption-questions-audit-c-by-using-ethyl-glucuronide-in-hair-hetg
#14
Monika Dreher-Weber, Anton-Rupert Laireiter, Anton Kühberger, Isabella Kunz, Michel Yegles, Tina Binz, Hans-Jürgen Rumpf, Rainer Hoffmann, Verena Schmidt, Siegfried Lang, Friedrich M Wurst
BACKGROUND: Because of physiological changes, elderly people are much more exposed to the adverse effects of alcohol. Therefore, hazardous drinking is defined at lower levels as compared to younger adults. This work aims to evaluate the validity of the current cut off levels of the Alcohol Use Disorder Identification Test - Consumption questions (AUDIT-C) to detect hazardous drinking in the elderly by using ethyl glucuronide in hair (HEtG). METHODS: In a border region between Austria and Germany, 344 nursing home residents were included from 33 of the 107 nursing homes...
July 18, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28716190/risk-factors-for-underweight-and-overweight-in-cats-in-metropolitan-sydney-australia
#15
Kendy T Teng, Paul D McGreevy, Jenny-Ann L M L Toribio, David Raubenheimer, Kim Kendall, Navneet K Dhand
Obesity is regarded as one of the most significant welfare issues in companion animals. Some risk factors affecting body condition in cats have been determined, but many remain controversial. The current study aimed to investigate the risk factors for overweight and underweight in cats in metropolitan Sydney. Electronic patient records for 11 years (2005-2015) were acquired from a feline primary practice in metropolitan Sydney. The 9-point body condition score (BCS) evaluation in each visit was classified into three groups: underweight (1-3), ideal-weight (4-6) and overweight (7-9)...
September 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28711791/labeled-azospirillum-brasilense-wild-type-and-excretion-ammonium-strains-in-association-with-barley-roots
#16
Adrian Richard Schenberger Santos, Rafael Mazer Etto, Rafaela Wiegand Furmam, Denis Leandro de Freitas, Karina Freire d'Eça Nogueira Santos, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Ricardo Antônio Ayub, Maria Berenice Reynaud Steffens, Carolina Weigert Galvão
Soil bacteria colonization in plants is a complex process, which involves interaction between many bacterial characters and plant responses. In this work, we labeled Azospirillum brasilense FP2 (wild type) and HM053 (excretion-ammonium) strains by insertion of the reporter gene gusA-kanamycin into the dinitrogenase reductase coding gene, nifH, and evaluated bacteria colonization in barley (Hordeum vulgare). In addition, we determined inoculation effect based on growth promotion parameters. We report an uncommon endophytic behavior of A...
July 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28711174/secondhand-smoke-exposure-and-preclinical-markers-of-cardiovascular-risk-in-toddlers
#17
Judith A Groner, Hong Huang, Mandar S Joshi, Nicholas Eastman, Lisa Nicholson, John Anthony Bauer
OBJECTIVE: To investigate relationships between secondhand smoke exposure in young children and several preclinical markers of cardiovascular risk that have been established as relevant to adult populations. STUDY DESIGN: There were 139 children, 2-5 years of age, enrolled in a cross-sectional study. Secondhand smoke exposure was objectively determined by hair nicotine level; a comprehensive panel of clinical markers (morning blood pressure, fasting glucose and insulin, lipid profiles, inflammation) and research markers (markers of oxidation, endothelial stress, and endothelial repair) of cardiovascular risk status were assessed...
July 12, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28709434/integrating-multi-omics-analyses-of-nonomuraea-dietziae-to-reveal-the-role-of-soybean-oil-in-4-oh-meleu-4-csa-overproduction
#18
Huanhuan Liu, Di Huang, Lina Jin, Cheng Wang, Shaoxiong Liang, Jianping Wen
BACKGROUND: Nonomuraea dietziae is a promising microorganism to mediate the region-specific monooxygenation reaction of cyclosporine A (CsA). The main product [(4'-OH)MeLeu](4)-CsA possesses high anti-HIV/HCV and hair growth-stimulating activities while avoiding the immunosuppressive effect of CsA. However, the low conversion efficiency restricts the clinical application. In this study, the production of [(4'-OH)MeLeu](4)-CsA was greatly improved by 55.6% from 182.8 to 284.4 mg/L when supplementing soybean oil into the production medium, which represented the highest production of [(4'-OH)MeLeu](4)-CsA so far...
July 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28709047/persistence-of-immersed-blood-and-hair-dna-a-preliminary-study-based-on-casework
#19
Christophe Frippiat, Agathe Gastaldi, Séverine Van Grunderbeeck
In some cases, evidence is collected from rivers, canals, lakes or sink pipes. To determine the utility of analyzing these samples and for cases in which DNA was recovered from submerged bulletproof vest parts, we evaluated the time necessary to degrade the blood and, subsequently, DNA on bulletproof vests. In a second experiment, also based on cases, blood was diluted in water from a kitchen sink pipe and incubated at room temperature for different times. Subsequently, DNA quality was assessed. In a parallel experiment, hair roots were incubated in spring water for different time periods...
July 5, 2017: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/28704045/local-environment-of-terbium-iii-ions-in-layered-nanocrystalline-zirconium-iv-phosphonate-phosphate-ion-exchange-materials
#20
Maxwell W Terban, Chenyang Shi, Rita Silbernagel, Abraham Clearfield, Simon J L Billinge
The structures of Zr(IV) phosphonate-phosphate based, unconventional metal organic framework materials have been determined using atomic pair distribution function analysis of high energy, X-ray total scattering diffraction data. They are found to form as nanocrystalline layers of Zr phosphate, similar to the bulk, but with a high degree of interlayer disorder and intermediate intralayer order extending around 5 nm. These materials are of interest for their high selectivity for 3+ lanthanide ions. To investigate the mechanism of the selectivity, we utilize difference pair distribution function analysis to extract the local structural environment of Tb(3+) ions loaded into the framework...
July 13, 2017: Inorganic Chemistry
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