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https://www.readbyqxmd.com/read/28636736/the-protective-effect-of-intratympanic-dexamethasone-on-streptomycin-ototoxicity-in-rats
#1
Aylin Gül, Engin Şengül, Beyhan Yılmaz, Fazıl Emre Özkurt, Mehmet Akdağ, Ayşenur Keleş, İsmail Topçu
The purpose of this experimental study was to investigate the protective role of intratympanically administered dexamethasone on the inner ears of rats that were exposed to streptomycin ototoxicity. Twenty-four adult Wistar albino rats were separated into 4 groups: Group 1 (only streptomycin), Group 2 (only intratympanic dexamethasone), Group 3 (streptomycin and intratympanic dexamethasone), and Group 4 (streptomycin and intratympanic saline). All rats were evaluated with distortion product otoacoustic emissions (DPOAE) tests before the start of treatment and on the day it ended...
June 2017: Ear, Nose, & Throat Journal
https://www.readbyqxmd.com/read/28630424/characterization-of-slow-cycling-corneal-limbal-epithelial-cells-identifies-putative-stem-cell-markers
#2
R Sartaj, C Zhang, P Wan, Z Pasha, V Guaiquil, A Liu, J Liu, Y Luo, E Fuchs, M I Rosenblatt
In order to identify reliable markers of corneal epithelial stem cells, we employed an inducible transgenic "pulse-chase" murine model (K5Tta × TRE-H2BGFP) to localize, purify, and characterize slow cycling cells in the cornea. The retention of GFP labeling in slowly dividing cells allowed for localization of these cells to the corneal limbus and their subsequent purification by FACS. Transcriptome analysis from slow cycling cells identified differentially expressed genes when comparing to GFP(-) faster-dividing cells...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28628851/spinal-5-ht4-and-5-ht6-receptors-contribute-to-the-maintenance-of-neuropathic-pain-in-rats
#3
Jorge Baruch Pineda-Farias, Paulino Barragán-Iglesias, Alann Valdivieso-Sánchez, Juan Rodríguez-Silverio, Francisco Javier Flores-Murrieta, Vinicio Granados-Soto, Héctor Isaac Rocha-González
BACKGROUND: Nerve injury promotes release of 5-HT at the spinal cord. Once released, 5-HT may produce antinociceptive or pronociceptive effects depending of the nature of 5-HT receptors. The purpose of this study was to investigate the participation of spinal 5-HT4 and 5-HT6 receptors in the maintenance of neuropathic pain in rats. METHODS: Tactile allodynia was measured using von Frey hairs in male Wistar rats subjected to L5-L6 spinal nerve injury. Selective 5-HT4 (GR-113808, 0...
April 4, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28624929/latex-peptidases-of-calotropis-procera-for-dehairing-of-leather-as-an-alternative-to-environmentally-toxic-sodium-sulfide-treatment
#4
Laura M I Lopéz, Carolina A Viana, María E Errasti, María L Garro, José E Martegani, Germán A Mazzilli, Cléverson D T Freitas, Ídila M S Araújo, Rafaela O da Silva, Márcio V Ramos
Dehairing of crude leather is a critical stage performed at the beginning of its processing to obtain industrially useful pieces. Tanneries traditionally apply a chemical process based on sodium sulfide. Since this chemical reactive is environmentally toxic and inefficiently recycled, innovative protocols for reducing or eliminating its use in leather depilation are welcomed. Therefore, latex peptidases from Calotropis procera (CpLP) and Cryptostegia grandiflora (CgLP) were assayed for this purpose. Enzyme activity on substrates representative of skin such as hide powder azure (UHPA), elastin (UE), azocollagen (UAZOCOL), keratin (UK), and epidermis (UEP) was determined, while depilation activity was assayed on cow hide...
June 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28624120/histologic-features-of-chronic-cutaneous-lupus-erythematosus-of-the-scalp-using-horizontal-sectioning-emphasis-on-follicular-findings
#5
Hye Jin Chung, Lynne J Goldberg
BACKGROUND: Chronic cutaneous lupus erythematosus (CCLE) often affects the scalp resulting in scarring alopecia. While histopathologic findings of CCLE have been well described, there is little written on the morphologic changes to the hair follicles in this condition. OBJECTIVE: We aim to determine the histopathologic findings of hair follicles in CCLE of the scalp. METHODS: We conducted a retrospective study of 33 transversely sectioned skin biopsy specimens of CCLE of the scalp at the Skin Pathology Laboratory at Boston University between April 2005 and March 2015...
June 14, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28618834/psychophysical-and-modeling-approaches-towards-determining-the-cochlear-phase-response-based-on-interaural-time-differences
#6
Hisaaki Tabuchi, Bernhard Laback
The cochlear phase response is often estimated by measuring masking of a tonal target by harmonic complexes with various phase curvatures. Maskers yielding most modulated internal envelope representations after passing the cochlear filter are thought to produce minimum masking, with fast-acting cochlear compression as the main contributor to that effect. Thus, in hearing-impaired (HI) listeners, reduced cochlear compression hampers estimation of the phase response using the masking method. This study proposes an alternative approach, based on the effect of the envelope modulation strength on the sensitivity to interaural time differences (ITDs)...
June 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28616244/macrophages-and-fibroblasts-during-inflammation-and-tissue-repair-in-models-of-organ-regeneration
#7
REVIEW
Anthony L Mescher
This review provides a concise summary of the changing phenotypes of macrophages and fibroblastic cells during the local inflammatory response, the onset of tissue repair, and the resolution of inflammation which follow injury to an organ. Both cell populations respond directly to damage and present coordinated sequences of activation states which determine the reparative outcome, ranging from true regeneration of the organ to fibrosis and variable functional deficits. Recent work with mammalian models of organ regeneration, including regeneration of full-thickness skin, hair follicles, ear punch tissues, and digit tips, is summarized and the roles of local immune cells in these systems are discussed...
April 2017: Regeneration
https://www.readbyqxmd.com/read/28612063/bioelements-in-hair-of-children-with-selected-neurological-disorders
#8
Jan Józefczuk, Wiktoria Kasprzycka, Rafał Czarnecki, Alfreda Graczyk, Paweł Józefczuk, Magda Krzysztof, Urszula Lampart, Ewa Mrozowska-Ząbek, Weronika Surdy, Róża Kwiatkowska-Graczyk
We have analyzed concentrations of magnesium (Mg), calcium (Ca), copper (Cu), zinc (Zn) and iron (Fe) in hair of a group of 82 children with mental retardation, in which 9 patients suffered from epilepsy, 18 from the Down's syndrome and 55 from cerebral palsy. Girls comprised little over 50% of the patients. In the group of boys with epilepsy, we found Mg, Ca, Cu and Fe deficiency, and normal level of Zn. In the group of girls with epilepsy, apart from low Fe concentration, a high level of Ca, Mg, Zn, and Cu was noted...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28611353/determination-of-arsenic-and-lead-in-single-hair-strands-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry
#9
Ruxin Luo, Xiaohong Su, Weicong Xu, Sujing Zhang, Xianyi Zhuo, Dong Ma
The purpose of this study was to develop matrix-matched hair standards and a LA-ICP-MS technique for determination of the As and Pb in a single human hair using single spot scan mode. These results could subsequently be used to infer when the element entered the body. This study was conducted in two parts. First, a method was developed and validated for the elemental analysis of hair by LA-ICP-MS. A calibration strategy in LA-ICP-MS was developed using prepared matrix-matched laboratory hair standards doped with analytes of interest at a defined concentration...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28601996/risk-assessment-for-human-health-in-a-seleniferous-area-shuang-an-china
#10
Zewei Cui, Jie Huang, Qin Peng, Dasong Yu, Songshan Wang, Dongli Liang
Shuang'an in Ziyang is a typical high-selenium (Se) area in China, where human selenium (Se) poisoning was reported 30 years ago. To assess the risk of Se poisoning in the area, the Se content in agricultural soil, plant, and water in Naore, Shuang Hekou, and Lin Benhe villages of Ziyang was systematically investigated. The probable daily intake (PDI) was calculated on the basis of Se contents in food, water, and dietary habits to evaluate the risk of selenosis. Se content in hair from local habitants (32 men and 34 women) was determined to further verify the risk...
June 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28598237/homocysteine-as-a-diagnostic-and-etiopathogenic-factor-in-children-with-autism-spectrum-disorder
#11
Jan Józefczuk, Wiktoria Kasprzycka, Rafał Czarnecki, Alfreda Graczyk, Paweł Józefczuk, Krzysztof Magda, Urszula Lampart
Substantial characteristics of autism are cognitive and psychophysical disorders. Etiopathogenetic factors are thought to be responsible for development of autism in children with genetic predisposition as well as have their effect on the severity of the disorders. The main problem of early identification of patients affected by autism spectrum disorder is that there are no clear diagnostic criteria. The aim of our study was assessment of hair magnesium and serum homocysteine concentrations in children with autism...
June 9, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28598071/-early-systemic-administration-of-il-10-inhibits-neuropathic-pain-in-adult-rats-with-tibial-nerve-injury
#12
Ya-Ping Feng, You-Quan Ding, Hong-Yi Ren, Xuan-Yang Li, Yang Qin, Jian-Guo Qi
OBJECTIVES: To determine the effect of early systemic administration of IL-10 on peripheral neuropathic pain induced by tibial nerve permanent transection [modified spared nerve injury (mSNI)]in adult rats. METHODS: Male adult Sprague-Dawley (SD) rats (ten-week old, 250-300 g) with mSNI were randomly divided into mSNI, sham-operated, IL-10 intervention (intraperitoneal injection), PBS intervention (intraperitoneal injection) groups, each containing six rats. Intraperitoneally injections (IL-10 or PBS) were given immediately after surgeries for a single regime with a dosage of 500 uL (0...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28597017/folliculotropic-mycosis-fungoides-with-skewed-t-cell-receptor-cdr3-motif-suggestive-of-lipid-antigen-selection
#13
Panagiota Mantaka, Agnieszka Malecka, Gunhild Trøen, Per Helsing, Petter Gjersvik, Klaus Beiske, Jan Delabie
Folliculotropic mycosis fungoides (FMF), a variant of mycosis fungoides (MF) with distinct clinical features, is characterized by infiltration of malignant T cells in hair follicles. This raises the hypothesis that antigens in the hair follicle may contribute to the pathogenesis of FMF. T-cell receptor β gene (TRB) sequences as well as dendritic cell subsets in patients with FMF (n = 21) and control patients with MF (n = 20) were studied to explore this hypothesis. A recurrent usage of the TRB junctional genes TRBJ2-1 and TRBJ2-7 was found in patients with FMF compared with those with MF...
June 9, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/28595997/hpv8-field-cancerization-in-a-transgenic-mouse-model-is-due-to-lrig1-keratinocyte-stem-cell-expansion
#14
Simone Lanfredini, Carlotta Olivero, Cinzia Borgogna, Federica Calati, Kathryn Powell, Kelli-Jo Davies, Marco De Andrea, Sarah Harries, Hiu Kwan Carolyn Tang, Herbert Pfister, Marisa Gariglio, Girish K Patel
β-HPV cause near ubiquitous latent skin infection within long-lived hair follicle keratinocyte stem cells (HF KSC). In patients with epidermodysplasia verruciformis (EV), β-HPV viral replication is associated with skin keratosis and cutaneous squamous cell carcinoma (SCC). To determine the role of HF KSC in β-HPV induced skin carcinogenesis, we utilized a transgenic mouse model in which the keratin 14 promoter drives expression of the entire HPV8 early region (HPV8tg). HPV8tg mice developed thicker skin in comparison to wild type littermates consistent with a hyperproliferative epidermis...
June 5, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28595479/psychobiology-of-cumulative-trauma-hair-cortisol-as-a-risk-marker-for-stress-exposure-in-women
#15
Matthew C Morris, James L Abelson, Alyssa S Mielock, Uma Rao
Childhood trauma (CT) is associated with long-lasting alterations of the hypothalamic-pituitary-adrenal (HPA) axis and elevated risk for stress exposure in adulthood. Although HPA alterations are present in the early aftermath of trauma, it remains unclear how initial HPA activity is associated with subsequent stress exposure and whether CT exposure influences the strength and direction of this association. The present study examined prospective associations between hair cortisol content (HCC) and stress exposure from baseline to 3-month follow-up in young adult women with recent (i...
June 8, 2017: Stress: the International Journal on the Biology of Stress
https://www.readbyqxmd.com/read/28593683/aging-in-hair-follicle-stem-cells-and-niche-microenvironment
#16
REVIEW
Jiang Ji, Bryan Siu-Yin Ho, Ge Qian, Xiao-Ming Xie, Paul Lorenz Bigliardi, Mei Bigliardi-Qi
Hair graying and hair loss are prominent and common characteristics of the elderly population. In some individuals these processes can significantly impact their quality of life, leading to depression, anxiety and other serious mental health problems. Accordingly, there has been much interest in understanding the complex physiological changes within the hair follicle in the aging individual. It is now known that hair follicles represent a prototypical stem cell niche, where both micro- and macroenvironmental influences are integrated alongside stem cell-stem cell and stem cell-stem niche interactions to determine hair growth or hair follicle senescence...
June 8, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28590835/a-pilot-study-on-the-evaluation-of-physicians-laser-delivery-performance-using-a-laser-beam-detection-kit
#17
Wooseok Koh, Hyoung-Woo Lim, Minwoo Cho, Dong-Hun Lee, Youdan Kim, Jin Ho Chung, Sungwan Kim
BACKGROUND AND OBJECTIVE: Many laser devices have been developed over the past decades for various skin conditions. However, variations in the technical skill of physicians for laser skin treatment delivery have not yet been evaluated. This study evaluates the differences in omission and overlap percentages during simulated laser hair removal treatments among physicians at two clinics. MATERIALS AND METHODS: A laser beam detection kit was developed to record and collect laser irradiation from a diode laser device...
June 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28586498/increased-hair-cortisol-concentrations-in-patients-with-progressive-keratoconus
#18
Janine Lenk, Eberhard Spoerl, Tobias Stalder, Susann Schmiedgen, Robert Herber, Lutz E Pillunat, Frederik Raiskup
PURPOSE: To investigate hair cortisol concentration (HCC), a biochemical correlate of chronic psychological stress, and its relationship to the progression of keratoconus. METHODS: Eighty-six participants were included in this prospective observational study (32 patients with progressive keratoconus, 32 patients with stable keratoconus, and 22 healthy controls). Hair cortisol concentrations were determined from 3-cm hair strands collected near the scalp from patients and controls as an index of cumulative cortisol secretion during the 3-month period prior to hair sampling...
June 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28583602/effects-of-environmental-temperature-and-season-on-hair-coat-characteristics-physiologic-and-reproductive-parameters-in-shetland%C3%A2-pony-stallions
#19
Kathrin Schmidt, Katharina Deichsel, Rodrigo Arruda de Oliveira, Jörg Aurich, Natascha Ille, Christine Aurich
We hypothesized that housing of stallions in a thermoneutral temperature zone during autumn and winter does not only influence metabolism and hair shedding but also improves the characteristics of raw and processed semen. Fertile Shetland pony stallions were followed from October to June. This time coincided with the seasons autumn, winter and spring. Ponies were kept in outside paddocks (group CON, n = 8) or in indoor stables (group ST, n = 8) from October to March when ST stallions returned to outdoor paddocks, but ponies remained in the same groups...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28580887/titanium-nanoparticle-size-influences-trace-concentration-levels-in-skin-appendages
#20
Deborah R Tasat, Mariela G Domingo, Marcos E Bruno, María B Guglielmotti, Daniel G Olmedo
As a result of biotribocorrosion, the surface of a titanium (Ti) biomedical device can be a potential source of systemic contamination with Ti nanoparticles (NPs). Although NPs can be chemically similar, differences in particle size may lead to different biological responses. The aim of this experimental study was to determine Ti trace levels in skin appendages and plasma and explore the influence of NP size on trace levels using a murine model. Results showed the presence of Ti traces in the nails, hair, and plasma...
January 1, 2017: Toxicologic Pathology
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