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https://www.readbyqxmd.com/read/28212389/application-of-calibrated-forceps-for-assessing-mechanical-nociception-with-high-time-resolution-in-mice
#1
Hideki Kashiwadani, Yuichi Kanmura, Tomoyuki Kuwaki
In order to investigate the basic physiological mechanisms of pain and the anti-nociceptive effects of analgesics, development of pain assays in mice is critical due to the advances of genetic manipulation techniques. The von Frey hairs/Semmes-Weinstein monofilaments test (von Frey test) has long been applied to examine mechanical nociception in mice. Though the von Frey test is a well-established and standardized method, it is inappropriate to assess a rapid change in the nociceptive threshold because voluntary resting/sleeping states are necessary to examine the response...
2017: PloS One
https://www.readbyqxmd.com/read/28210756/high-precision-zinc-stable-isotope-measurement-of-certified-biological-reference-materials-using-the-double-spike-technique-and-multiple-collector-icp-ms
#2
Rebekah E T Moore, Fiona Larner, Barry J Coles, Mark Rehkämper
Biological reference materials with well-characterised stable isotope compositions are lacking in the field of 'isotope biochemistry', which seeks to understand bodily processes that rely on essential metals by determining metal stable isotope ratios. Here, we present Zn stable isotope data for six biological reference materials with certified trace metal concentrations: fish muscle, bovine muscle, pig kidney, human hair, human blood serum and human urine. Replicate analyses of multiple aliquots of each material achieved reproducibilities (2sd) of 0...
February 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28209736/pejvakin-a-candidate-stereociliary-rootlet-protein-regulates-hair-cell-function-in-a-cell-autonomous-manner
#3
Marcin Kazmierczak, Piotr Kazmierczak, Anthony W Peng, Suzan L Harris, Prahar Shah, Jean-Luc Puel, Marc Lenoir, Santos J Franco, Martin Schwander
Mutations in the Pejvakin (PJVK) gene are thought to cause auditory neuropathy and hearing loss of cochlear origin by affecting noise-induced peroxisome proliferation in auditory hair cells and neurons. Here we demonstrate that loss of pejvakin in hair cells, but not in neurons, causes profound hearing loss and outer hair cell degeneration in mice. Pejvakin binds to and co-localizes with the rootlet component TRIOBP at the base of stereocilia in injectoporated hair cells, a pattern that is disrupted by deafness-associated PJVK mutations...
February 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28205242/one-way-or-another-iron-uptake-in-plants
#4
Huei-Hsuan Tsai, Wolfgang Schmidt
Iron (Fe) and phosphorus (P), the latter taken up by plants as phosphate (Pi), are two essential nutrients that determine species distribution and often limit crop yield as a result of their low availability in most soils. Pi-deficient plants improve the interception of Pi by increasing the density of root hairs, thereby expanding the volume of soil to be explored. The increase in root hair frequency results mainly from attenuated primary root growth, a process that was shown to be dependent on the availability of external Fe...
February 16, 2017: New Phytologist
https://www.readbyqxmd.com/read/28203534/comparison-of-methanol-and-isopropanol-as-wash-solvents-for-determination-of-hair-cortisol-concentration-in-grizzly-bears-and-polar-bears
#5
Thomas Kroshko, Luciene Kapronczai, Marc R L Cattet, Bryan J Macbeth, Gordon B Stenhouse, Martyn E Obbard, David M Janz
Methodological differences among laboratories are recognized as significant sources of variation in quantification of hair cortisol concentration (HCC). An important step in processing hair, particularly when collected from wildlife, is the choice of solvent used to remove or "wash" external hair shaft cortisol prior to quantification of HCC. The present study systematically compared methanol and isopropanol as wash solvents for their efficiency at removing external cortisol without extracting internal hair shaft cortisol in samples collected from free-ranging grizzly bears and polar bears...
2017: MethodsX
https://www.readbyqxmd.com/read/28202575/models-of-utricular-bouton-afferents-role-of-afferent-hair-cell-connectivity-in-determining-spike-train-regularity
#6
William R Holmes, Janice A Huwe, Barbara Williams, Michael H Rowe, Ellengene H Peterson
Vestibular bouton afferent terminals in turtle utricle can be categorized into four types depending on their location and terminal arbor structure: lateral extrastriolar (LES), striolar, juxtastriolar, and medial extrastriolar (MES). The terminal arbors of these afferents differ in surface area, total length, collecting area, number of boutons, number of bouton contacts per hair cell and axon diameter (Huwe et al. 2015). To understand how differences in terminal morphology and the resulting hair cell inputs might affect afferent response properties, we modeled representative afferents from each region, using reconstructed bouton afferents (Huwe et al...
February 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28199947/environmental-biomonitoring-of-essential-and-toxic-elements-in-human-scalp-hair-using-accelerated-microwave-assisted-sample-digestion-and-inductively-coupled-plasma-optical-emission-spectroscopy
#7
Hope Kumakli, A'ja V Duncan, Kiara McDaniel, Tsdale F Mehari, Jamira Stephenson, Lareisha Maple, Zaria Crawford, Calvin L Macemore, Carol M Babyak, Sayo O Fakayode
Human scalp hair samples were collected and used to assess exposure to toxic elements and essential elements in the state of North Carolina, USA using accelerated microwave assisted acid digestion and inductively coupled plasma optical emission spectroscopy (ICP-OES). The figures-of-merit of the ICP-OES were appropriate for elemental analysis in scalp hair with detection limits as low as 0.0001 mg/L for Cd, good linearity (R(2) > 0.9978), and percent recoveries that ranged from 96 to 106% for laboratory-fortified-blanks and 88-112% for sample spike recovery study...
February 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28199938/assessment-of-multiple-exposure-to-chemical-elements-and-health-risks-among-residents-near-huodehong-lead-zinc-mining-area-in-yunnan-southwest-china
#8
Yirun Wang, Ruming Wang, Liyun Fan, Tiantian Chen, Yahong Bai, Qianru Yu, Ying Liu
The contents of chemical elements (Cd, Cr, Cu, Pb and Zn) in 11 kinds of crop/vegetables and soils around the Huodehong lead-zinc mining area in Yunnan, Southwest China were determined by using inductive coupled plasma emission spectrometry (ICP-MS). Results showed that element contents in soils decreased in the order of Zn > Pb > Cr > Cu > Cd. The high geo-accumulation indexes (Igeo) showed that cultivated soils near mine were practically polluted by Cd, Pb and Zn. The contents of Cd, Cr and Pb in crop/vegetables samples were significantly higher than the maximum permissible standard set by China...
January 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28195092/efficacy-of-in-house-fluorescent-stain-for-fungus
#9
K R L Surya Kirani, V Satya Chandrika
CONTEXT: Mycotic infections are gaining importance in the present day medicine, and definite demonstration of fungus is essential for diagnosis. Small numbers of organisms in the smear can be identified by fluorescence microscopy. Calcofluor white (CFW) fluorescent stain is a textile brightener mixed with Evans blue. It is expensive and not easily available. AIMS: (1) To assess the efficacy of in-house CFW fluorescent stain for fungus in relation to conventional CFW stain, histopathology, and culture...
January 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28193226/hair-growth-promoting-effect-of-geranium-sibiricum-extract-in-human-dermal-papilla-cells-and-c57bl-6-mice
#10
William A Boisvert, Miri Yu, Youngbin Choi, Gi Hee Jeong, Yi-Lin Zhang, Sunghun Cho, Changsun Choi, Sanghyun Lee, Bog-Hieu Lee
BACKGROUND: Geranium sibiricum L. has been used as a medicinal plant to treat diarrhea, bacterial infection, and cancer in Bulgaria, Peru, and Korea. However, its hair growth-promoting effect was not investigated so far. This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models. METHODS: Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). Hair-growth promoting effect was measured in animal model...
February 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28192143/lrrc8a-dependent-volume-regulated-anion-channel-activity-is-dispensable-for-t-cell-development-and-function
#11
Craig D Platt, Janet Chou, Patrick Houlihan, Yousef R Badran, Lalit Kumar, Wayne Bainter, P Luigi Poliani, Carlos J Perez, Sharon Y R Dent, David E Clapham, Fernando Benavides, Raif S Geha
BACKGROUND: Leucine-rich repeat containing 8A (LRRC8A) is a ubiquitously expressed transmembrane protein with 17 LRRs at its C-terminal end, and is an essential component of the volume regulated anion channel (VRAC) which controls cellular volume. A heterozygous mutation in LRRC8A that truncates the two terminal LRRs was reported in a patient with agammaglobulinemia and absent B cells, and was demonstrated to exert a dominant negative effect on T and B cell development in mice. Lrrc8a(-/-) mice have severely defective T cell development and function...
February 9, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28176951/in-vitro-and-in-vivo-evaluation-of-biologically-synthesized-silver-nanoparticles-for-topical-applications-effect-of-surface-coating-and-loading-into-hydrogels
#12
Aml I Mekkawy, Mohamed A El-Mokhtar, Nivien A Nafady, Naeima Yousef, Mostafa A Hamad, Sohair M El-Shanawany, Ehsan H Ibrahim, Mahmoud Elsabahy
In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and β-CD-coated and uncoated AgNPs, respectively...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28170296/muse-cells-derived-from-dermal-tissues-can-differentiate-into-melanocytes
#13
Ting Tian, Ru-Zhi Zhang, Yu-Hua Yang, Qi Liu, Di Li, Xiao-Ru Pan
The objective of the authors has been to obtain multilineage-differentiating stress-enduring cells (Muse cells) from primary cultures of dermal fibroblasts, identify their pluripotency, and detect their ability to differentiate into melanocytes. The distribution of SSEA-3-positive cells in human scalp skin was assessed by immunohistochemistry, and the distribution of Oct4, Sox2, Nanog, and SSEA-3-positive cells was determined by immunofluorescence staining. The expression levels of Sox2, Oct4, hKlf4, and Nanog mRNAs and proteins in Muse cells were determined by reverse transcription polymerase chain reaction (RT-PCR) analyses and Western blots, respectively...
February 7, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28156639/determining-priority-symptoms-of-women-with-recurrent-ovarian-cancers-a-gynecologic-oncology-group-study
#14
Lari B Wenzel, Sandra Ward, Susan M Sereika, Robert P Edwards, Michael Spring, Catherine M Bender, Richard T Penson, Teresa Hagan
: 101 Background: The purpose of this study is to recommend a core set of priority symptoms to be assessed in research and clinical settings for women with recurrent ovarian cancers. METHODS: We used baseline data of 497 women with recurrent ovarian, fallopian, or primary peritoneal cancer participating in a symptom management randomized clinical trial (GOG-0259) to identify a core index of patient-reported priority symptoms. We used the Symptom Representation Questionnaire to assess priority rankings of 28 symptoms based on four criteria: (1) symptom prevalence, (2) patient-reported symptom severity, (3) percentage of women identifying each symptom as one of top three symptoms "I would like to get better control over", and (4) the association between symptom severity and functional wellbeing as measured by the Functional Assessment of Cancer Therapy - Ovarian...
October 9, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28155290/the-diagnosis-of-bilateral-primary-renal-paragangliomas-in-a-cat
#15
Ryan B Friedlein, Alain J Carter, Robert D Last, Sarah Clift
A 9-year-old sterilised female domestic short-hair cat was referred with a history of vomiting and anorexia of 3 months' duration. Biochemistry, full-blood counts, thoracic radiographs, feline pancreatic-specific lipase, abdominal ultrasonography and feline immunodeficiency virus/feline leukaemia virus (FIV/FeLV) SNAP tests had been performed. Mild hypochloraemia and moderate hypokalaemia were evident on initial presentation. Abdominal ultrasonography initially revealed unilateral renal nodules on the left side...
January 24, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28155261/dietary-protein-content-and-tissue-type-control-13-c-discrimination-in-mammals-an-analytical-approach
#16
Erik A Hobbie
RATIONALE: Carbon isotope measurements on collagen and other tissues are used in modern and paleodiet studies, and relating these patterns to the dietary protein fraction (fdp ) and δ(13) C values of dietary protein and energy (lipids and carbohydrates) remains important for determining diets. METHODS: Carbon fluxes from dietary protein to tissues in feeding studies of mice, rats, and pigs were estimated to test how tissue δ(13) C values related to the δ(13) C values of dietary protein, dietary energy, fdp , or loge fdp ...
February 3, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28155080/serum-and-hair-nickel-levels-and-breast-cancer-systematic-review-and-meta-analysis
#17
Min Yu, Juan Zhang
Several studies have investigated the relationship between serum and hair nickel (Ni) concentration and breast cancer, but the results were inconsistent. Thus, we conducted a systematic review and meta-analysis to evaluate the association between serum and hair nickel levels and breast cancer. Nine studies determining the serum nickel levels and six studies evaluating the hair nickel levels were identified in a systematic search of PubMed, China Knowledge Resource Integrated Database, Wanfang, and China Biomedical Literature Service System databases...
February 2, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28135674/stress-related-and-basic-determinants-of-hair-cortisol-in-humans-a-meta-analysis
#18
REVIEW
Tobias Stalder, Susann Steudte-Schmiedgen, Nina Alexander, Tim Klucken, Annika Vater, Susann Wichmann, Clemens Kirschbaum, Robert Miller
The analysis of hair cortisol concentrations (HCC) is a relatively new strategy to measure long-term cumulative cortisol levels, which is increasingly used in psychoneuroendocrinological research. Here, we conduct a first comprehensive meta-analysis of HCC research based on aggregated data from a total of 124 (sub)samples (66 independent studies; total N=10,289). We seek to answer two central questions: (i) Which covariates and basic features of HCC need to be considered in future research? (ii) What are the main determinants of HCC in terms of chronic stress exposure and mental health? Concerning basic characteristics, our findings identify several covariates to be considered (age, sex, hair washing frequency, hair treatment, oral contraceptive use), confirm a decline of HCC from the first to the second proximal 3cm hair segment, and show positive associations between HCC and short-term salivary cortisol measures...
January 10, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28130742/assessment-of-mercury-intake-from-fish-meals-based-on-intervention-research-in-the-polish-subpopulation
#19
Renata Kuras, Beata Janasik, Magdalena Stanislawska, Lucyna Kozlowska, Wojciech Wasowicz
The paper's objective was to estimate weekly Hg intake from fish meals based on intervention research. Total Hg (THg) concentrations in blood and hair samples collected from men (n = 67) from an intervention study as well as muscular tissues of fresh and after heat-treating fish were determined using the thermal decomposition amalgamation atomic absorption spectrometry method (TDA-AAS) using direct mercury analyzer (DMA-80). The mean of the estimated weekly intake (EWI) was estimated at 0.62 μg/kg bw/week in the range 0...
January 27, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28126377/defects-in-lymphocyte-telomere-homeostasis-contribute-to-cellular-immune-phenotype-in-cartilage-hair-hypoplasia
#20
Geraldine Aubert, Kevin A Strauss, Peter M Lansdorp, Nicholas L Rider
BACKGROUND: Mutations in the lncRNA RNase component of the Mitochondrial RNA Processing endoribonuclease (RMRP) give rise to the autosomal recessive condition cartilage-hair hypoplasia (CHH). The CHH disease phenotype has some overlap with dyskeratosis congenita, a well known "telomere disorder". RMRP binds the telomerase reverse transcriptase protein TERT in some cell lines, raising the possibility that RMRP may play a role in telomere biology. OBJECTIVE: We sought to determine if a telomere phenotype is present in immune cells from individuals with CHH and explore mechanisms underlying these observations...
January 23, 2017: Journal of Allergy and Clinical Immunology
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