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Gene expression profile of human plaque

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https://www.readbyqxmd.com/read/27818938/modulation-of-ambient-temperature-promotes-inflammation-and-initiates-atherosclerosis-in-wild-type-c57bl-6-mice
#1
Daniel A Giles, Bhama Ramkhelawon, Elizabeth M Donelan, Traci E Stankiewicz, Susan B Hutchison, Rajib Mukherjee, Monica Cappelletti, Rebekah Karns, Christopher L Karp, Kathryn J Moore, Senad Divanovic
OBJECTIVES: Obesity and obesity-associated inflammation is central to a variety of end-organ sequelae including atherosclerosis, a leading cause of death worldwide. Although mouse models have provided important insights into the immunopathogenesis of various diseases, modeling atherosclerosis in mice has proven difficult. Specifically, wild-type (WT) mice are resistant to developing atherosclerosis, while commonly used genetically modified mouse models of atherosclerosis are poor mimics of human disease...
November 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27727291/early-transcriptomic-response-to-ldl-and-oxldl-in-human-vascular-smooth-muscle-cells
#2
Salvador Damián-Zamacona, Paola Toledo-Ibelles, Mabel Z Ibarra-Abundis, Laura Uribe-Figueroa, Enrique Hernández-Lemus, Karla Paola Macedo-Alcibia, Blanca Delgado-Coello, Jaime Mas-Oliva, Juan Pablo Reyes-Grajeda
BACKGROUND: Although nowadays it is well known that the human transcriptome can importantly vary according to external or environmental condition, the reflection of this concept when studying oxidative stress and its direct relationship with gene expression profiling during the process of atherogenesis has not been thoroughly achieved. OBJECTIVE: The ability to analyze genome-wide gene expression through transcriptomics has shown that the genome responds dynamically to diverse stimuli...
2016: PloS One
https://www.readbyqxmd.com/read/27707922/syncytial-mutations-do-not-impair-the-specificity-of-entry-and-spread-of-a-glycoprotein-d-receptor-retargeted-herpes-simplex-virus
#3
Yu Okubo, Hiroaki Uchida, Aika Wakata, Takuma Suzuki, Tomoko Shibata, Hitomi Ikeda, Miki Yamaguchi, Justus B Cohen, Joseph C Glorioso, Mitsuo Tagaya, Hirofumi Hamada, Hideaki Tahara
: Membrane fusion, which is the key process for both initial cell entry and subsequent lateral spread of herpes simplex virus (HSV), requires the four envelope glycoproteins gB, gD, gH, and gL. Syncytial mutations, predominantly mapped to the gB and gK genes, confer hyperfusogenicity on HSV and cause multinucleated giant cells, termed syncytia. Here, we asked whether interaction of gD with a cognate entry receptor remains indispensable for initiating membrane fusion of syncytial strains...
October 5, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27513102/tissue-engineered-human-psoriatic-skin-supplemented-with-cytokines-as-an-in%C3%A2-vitro-model-to-study-plaque-psoriasis
#4
Claudia Pouliot-Bérubé, Karine Zaniolo, Sylvain L Guérin, Roxane Pouliot
AIM: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. MATERIALS & METHODS: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. RESULTS: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU)...
September 2016: Regenerative Medicine
https://www.readbyqxmd.com/read/27400380/metagenome-and-metatranscriptome-analyses-using-protein-family-profiles
#5
Cuncong Zhong, Anna Edlund, Youngik Yang, Jeffrey S McLean, Shibu Yooseph
Analyses of metagenome data (MG) and metatranscriptome data (MT) are often challenged by a paucity of complete reference genome sequences and the uneven/low sequencing depth of the constituent organisms in the microbial community, which respectively limit the power of reference-based alignment and de novo sequence assembly. These limitations make accurate protein family classification and abundance estimation challenging, which in turn hamper downstream analyses such as abundance profiling of metabolic pathways, identification of differentially encoded/expressed genes, and de novo reconstruction of complete gene and protein sequences from the protein family of interest...
July 2016: PLoS Computational Biology
https://www.readbyqxmd.com/read/27350674/improving-lipoprotein-profiles-by-liver-directed-gene-transfer-of-low-density-lipoprotein-receptor-gene-in-hypercholesterolaemia-mice
#6
Hailong Ou, Qinghai Zhang, Jia Zeng
The defect of low density lipoprotein receptor disturbs cholesterol metabolism and causes familial hypercholesterolaemia (FH). In this study, we directly delivered exogenous Ldlr gene into the liver of FH model mice (Ldlr(-/-)) by lentiviral gene transfer system. The results showed that the Ldlr gene controlled by hepatocyte-specific human thyroxine-binding globulin (TBG) promoter successfully and exclusively expressed in livers.We found that, although, the content of high density lipoprotein in serum was not significantly affected by the Ldlr gene expression, the serum low density lipoprotein level was reduced by 46%, associated with a 30% and 28% decrease in triglyceride and total cholesterol, respectively, compared to uninjected Ldlr(-/-) mice...
June 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27180200/amendment-of-the-cytokine-profile-in-macrophages-subsequent-to-their-interaction-with-smooth-muscle-cells-differential-modulation-by-fractalkine-and-resistin
#7
Monica Madalina Tucureanu, Elena Butoi, Ana-Maria Gan, Daniela Stan, Cristina Ana Constantinescu, Manuela Calin, Maya Simionescu, Ileana Manduteanu
In atherosclerotic plaques, macrophages (MAC) and smooth muscle cells (SMC) frequently reside in close proximity and resistin (Rs) and fractalkine (Fk) are present at increased levels, resistin being associated with CD68 macrophages and fractalkine predominantly associated with intimal SMC; however, their role in this location is not clear, yet. The objective of this study was to determine whether the cross-talk between MAC-SMC induces changes in MAC cytokine phenotype and if Fk and Rs have a role in the process...
July 2016: Cytokine
https://www.readbyqxmd.com/read/27124409/porphyromonas-gingivalis-differentially-modulates-cell-death-profile-in-ox-ldl-and-tnf-%C3%AE-pre-treated-endothelial-cells
#8
Isaac Maximiliano Bugueno, Yacine Khelif, Narendra Seelam, David-Nicolas Morand, Henri Tenenbaum, Jean-Luc Davideau, Olivier Huck
OBJECTIVE: Clinical studies demonstrated a potential link between atherosclerosis and periodontitis. Porphyromonas gingivalis (Pg), one of the main periodontal pathogen, has been associated to atheromatous plaque worsening. However, synergism between infection and other endothelial stressors such as oxidized-LDL or TNF-α especially on endothelial cell (EC) death has not been investigated. This study aims to assess the role of Pg on EC death in an inflammatory context and to determine potential molecular pathways involved...
2016: PloS One
https://www.readbyqxmd.com/read/27058954/amyloid-beta-mediated-hypomethylation-of-heme-oxygenase-1-correlates-with-cognitive-impairment-in-alzheimer-s-disease
#9
Hye Youn Sung, Byung-Ok Choi, Jee Hyang Jeong, Kyoung Ae Kong, Jinha Hwang, Jung-Hyuck Ahn
To identify epigenetically regulated genes involved in the pathogenesis of Alzheimer's disease (AD) we analyzed global mRNA expression and methylation profiles in amyloid precursor protein (APP)-Swedish mutant-expressing AD model cells, H4-sw and selected heme oxygenase-1 (HMOX1), which is associated with pathological features of AD such as neurofibrillary tangles and senile plaques. We examined the epigenetic regulatory mechanism of HMOX1 and its application as a diagnostic and prognostic biomarker for AD...
2016: PloS One
https://www.readbyqxmd.com/read/26936520/regulation-of-global-gene-expression-and-cell-proliferation-by-app
#10
Yili Wu, Si Zhang, Qin Xu, Haiyan Zou, Weihui Zhou, Fang Cai, Tingyu Li, Weihong Song
Down syndrome (DS), caused by trisomy of chromosome 21, is one of the most common genetic disorders. Patients with DS display growth retardation and inevitably develop characteristic Alzheimer's disease (AD) neuropathology, including neurofibrillary tangles and neuritic plaques. The expression of amyloid precursor protein (APP) is increased in both DS and AD patients. To reveal the function of APP and elucidate the pathogenic role of increased APP expression in DS and AD, we performed gene expression profiling using microarray method in human cells overexpressing APP...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26842798/integration-of-flow-studies-for-robust-selection-of-mechanoresponsive-genes
#11
Nataly Maimari, Ryan M Pedrigi, Alessandra Russo, Krysia Broda, Rob Krams
Blood flow is an essential contributor to plaque growth, composition and initiation. It is sensed by endothelial cells, which react to blood flow by expressing > 1000 genes. The sheer number of genes implies that one needs genomic techniques to unravel their response in disease. Individual genomic studies have been performed but lack sufficient power to identify subtle changes in gene expression. In this study, we investigated whether a systematic meta-analysis of available microarray studies can improve their consistency...
March 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/26663183/micrornas-in-cerebrovascular-disease
#12
REVIEW
Ondřej Volný, Linda Kašičková, Dominika Coufalová, Petra Cimflová, Jan Novák
Cardiovascular diseases are major causes of morbidity and mortality in developed countries. Cerebrovascular diseases, especially stroke, represent major burden of disability and economy impact. Major advances in primary and secondary prevention and therapy are needed in order to tackle this public health problem. Our better understanding of pathophysiology is essential in order to develop novel diagnostic and therapeutic tools and strategies. microRNAs are a family of important post-transcriptional regulators of gene expression and their involvement in the pathophysiology of cerebrovascular diseases has already been reported...
2015: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/26619277/polymicrobial-oral-infection-with-four-periodontal-bacteria-orchestrates-a-distinct-inflammatory-response-and-atherosclerosis-in-apoe-null-mice
#13
Sasanka S Chukkapalli, Irina M Velsko, Mercedes F Rivera-Kweh, Donghang Zheng, Alexandra R Lucas, Lakshmyya Kesavalu
Periodontal disease (PD) develops from a synergy of complex subgingival oral microbiome, and is linked to systemic inflammatory atherosclerotic vascular disease (ASVD). To investigate how a polybacterial microbiome infection influences atherosclerotic plaque progression, we infected the oral cavity of ApoE null mice with a polybacterial consortium of 4 well-characterized periodontal pathogens, Porphyromonas gingivalis, Treponema denticola, Tannerealla forsythia and Fusobacterium nucleatum, that have been identified in human atherosclerotic plaque by DNA screening...
2015: PloS One
https://www.readbyqxmd.com/read/26268531/iatrogenic-cjd-due-to-pituitary-derived-growth-hormone-with-genetically-determined-incubation-times-of-up-to-40-years
#14
Peter Rudge, Zane Jaunmuktane, Peter Adlard, Nina Bjurstrom, Diana Caine, Jessica Lowe, Penny Norsworthy, Holger Hummerich, Ron Druyeh, Jonathan D F Wadsworth, Sebastian Brandner, Harpreet Hyare, Simon Mead, John Collinge
Patients with iatrogenic Creutzfeldt-Jakob disease due to administration of cadaver-sourced growth hormone during childhood are still being seen in the UK 30 years after cessation of this treatment. Of the 77 patients who have developed iatrogenic Creutzfeldt-Jakob disease, 56 have been genotyped. There has been a marked change in genotype profile at polymorphic codon 129 of the prion protein gene (PRNP) from predominantly valine homozygous to a mixed picture of methionine homozygous and methionine-valine heterozygous over time...
November 2015: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/26088578/dna-methylation-dynamics-in-human-carotid-plaques-after-cerebrovascular-events
#15
COMPARATIVE STUDY
Silvio Zaina, Isabel Gonçalves, F Javier Carmona, Antonio Gomez, Holger Heyn, Inês G Mollet, Sebastian Moran, Nuray Varol, Manel Esteller
OBJECTIVE: To understand whether cerebrovascular events, a major complication of atherosclerosis, are associated with any specific DNA methylation changes in the carotid plaque. APPROACH AND RESULTS: We profiled the DNA methylomes of human symptomatic carotid plaques obtained from patients who had cerebrovascular events (n=19) and asymptomatic counterparts (n=19) with a high-density microarray (≈485 000 CpG sites, Illumina), and crossed DNA methylation data with RNAseq-based expression data from an independent symptomatic carotid plaque set (n=8)...
August 2015: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/26071899/rna-sequencing-reveals-transcriptional-up-regulation-of-trem2-in-response-to-bexarotene-treatment
#16
Iliya Lefterov, Jonathan Schug, Anais Mounier, Kyong Nyon Nam, Nicholas F Fitz, Radosveta Koldamova
We have recently demonstrated that short term bexarotene treatment of APP/PS1 mice significantly improves their cognitive performance. While there were no changes in plaque load, or insoluble Aβ levels in brain, biochemical analysis strongly suggested improved clearance of soluble Aβ, including Aβ oligomers. To get further insight into molecular mechanisms underlying this therapeutic effect, we explored genome-wide differential gene expression in brain of bexarotene and control treated APP/PS1 mice. We performed high throughput massively parallel sequencing on mRNA libraries generated from cortices of bexarotene or vehicle treated APP/PS1 mice and compared the expression profiles for differential gene expression...
October 2015: Neurobiology of Disease
https://www.readbyqxmd.com/read/25990243/inflammatory-environment-and-oxidized-ldl-convert-circulating-human-proangiogenic-cells-into-functional-antigen-presenting-cells
#17
Maria Cristina Vinci, Luca Piacentini, Mattia Chiesa, Federica Saporiti, Gualtiero I Colombo, Maurizio Pesce
The function of human circulating PACs has been described extensively. However, little focus has been placed on understanding how these cells differ in their functions in the presence of microenvironments mimicking vascular inflammation. We hypothesized that exposure to proinflammatory cytokines or the oxLDL, an autoantigen abundant in advanced atherosclerotic plaques, converts PACs into immune-modulating/proinflammatory cells. Hence, we examined the effect of oxLDL and inflammatory stimuli on their phenotype by use of a functional genomics model based on secretome and whole genome transcriptome profiling...
September 2015: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/25948119/identification-by-microarray-technology-of-key-genes-involved-in-the-progression-of-carotid-atherosclerotic-plaque
#18
COMPARATIVE STUDY
Jian Wang, Bo Wei, Shuwei Cao, Fengke Xu, Wei Chen, Haifeng Lin, Chenghua Du, Zhigang Sun
A comparative analysis of gene expression profiles between early and advanced carotid atherosclerotic plaque was performed to identify key genes and pathways involved in the progression of carotid atherosclerotic plaque. Gene expression data set GSE28829 was downloaded from Gene Expression Omnibus, including 13 early and 16 advanced atherosclerotic plaque samples from human carotid. Differentially expressed genes (DEGs) were identified using the package limma of R. Principal component analysis was carried out for the DEGs with package rgl of R...
2014: Genes & Genetic Systems
https://www.readbyqxmd.com/read/25889164/effects-of-statin-on-circulating-micrornaome-and-predicted-function-regulatory-network-in-patients-with-unstable-angina
#19
Jingjin Li, Hong Chen, Jingyi Ren, Junxian Song, Feng Zhang, Jing Zhang, Chongyou Lee, Sufang Li, Qiang Geng, Chengfu Cao, Ning Xu
BACKGROUND: Statin therapy plays a pivotal role in stabilizing the plaque for unstable angina (UA) patients although its mechanism(s) remains largely unexplored. Here we aim to identify microRNAs (miRNAs) mediating the protective effect of statins in UA patients. METHODS: MiRNAs Array was carried out to compare the circulating whole blood miRNA profile of UA patients treated with (n = 10) and without statin (n = 10) and plasma miRNA profile UA patients treated with (n = 5) and without statin (n = 5)...
2015: BMC Medical Genomics
https://www.readbyqxmd.com/read/25825525/modulation-of-macrophage-gene-expression-via-liver-x-receptor-%C3%AE-serine-198-phosphorylation
#20
Chaowei Wu, Maryem A Hussein, Elina Shrestha, Sarah Leone, Mohammed S Aiyegbo, W Marcus Lambert, Benoit Pourcet, Timothy Cardozo, Jan-Ake Gustafson, Edward A Fisher, Ines Pineda-Torra, Michael J Garabedian
In mouse models of atherosclerosis, normalization of hyperlipidemia promotes macrophage emigration and regression of atherosclerotic plaques in part by liver X receptor (LXR)-mediated induction of the chemokine receptor CCR7. Here we report that LXRα serine 198 (S198) phosphorylation modulates CCR7 expression. Low levels of S198 phosphorylation are observed in plaque macrophages in the regression environment where high levels of CCR7 expression are observed. Consistent with these findings, CCR7 gene expression in human and mouse macrophages cell lines is induced when LXRα at S198 is nonphosphorylated...
June 1, 2015: Molecular and Cellular Biology
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