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autophagy ischemia

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https://www.readbyqxmd.com/read/29787987/modulation-of-autophagy-as-new-approach-in-mesenchymal-stem-cell-based-therapy
#1
REVIEW
Jelena Jakovljevic, C Randall Harrell, Crissy Fellabaum, Aleksandar Arsenijevic, Nemanja Jovicic, Vladislav Volarevic
Due to their trophic and immunoregulatory characteristics mesenchymal stem cells (MSCs) have tremendous potential for use in a variety of clinical applications. Challenges in MSCs' clinical applications include low survival of transplanted cells and low grafting efficiency requiring use of a high number of MSCs to achieve therapeutic benefits. Accordingly, new approaches are urgently needed in order to overcome these limitations. Recent evidence indicates that modulation of autophagy in MSCs prior to their transplantation enhances survival and viability of engrafted MSCs and promotes their pro-angiogenic and immunomodulatory characteristics...
May 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29787772/ginkgolide-k-promotes-astrocyte-proliferation-and-migration-after-oxygen-glucose-deprivation-via-inducing-protective-autophagy-through-the-ampk-mtor-ulk1-signaling-pathway
#2
Ying Zhang, Ju-Mei Miao
Ischemic stroke is the leading cause of death around the world. Ginkgolide K (GK) has been used to treat ischemic stroke due to its neuroprotective potential. However, the molecular mechanism underlying the neuroprotective effect of GK in ischemic stroke is still almost blank. In this study, astrocytes were divided into four groups: control group, oxygen-glucose deprivation (OGD) group, OGD + GK group and OGD + GK + Compound C (CC) group. The viability and proliferation of astrocytes were examined by Cell Counting Kit-8 assay and 5-ethynyl-20-deoxyuridine (EdU) assay, respectively...
May 19, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29777940/curcumin-attenuates-cerebral-ischemia-injury-in-sprague-dawley-rats-and-pc12-cells-by-suppressing-overactivated-autophagy
#3
Yan Zhang, Ming Fang, Yameng Sun, Ting Zhang, Nan Shi, Jinfeng Li, Lei Jin, Kangyong Liu, Jianliang Fu
The present study assessed whether the protective effects of curcumin against cerebral ischemia injury were due to the suppression of overactivated autophagy. Curcumin is a well-known natural polyphenolic compound that effectively counteracts oxidation, inflammation, and various types of cancer. Several studies have demonstrated the protective effects of curcumin against ischemia-reperfusion injury in tissues from the lungs, cardiomyocytes, and liver. The present study employed brain injury models induced by middle cerebral artery occlusion (MCAO) in rats and PC12 oxygen-glucose-deprived (OGD) cells...
May 7, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29775658/hydroxysafflor-yellow-a-protects-brain-microvascular-endothelial-cells-against-oxygen-glucose-deprivation-reoxygenation-injury-involvement-of-inhibiting-autophagy-via-class-i-pi3k-akt-mtor-signaling-pathway
#4
Guang Yang, Ning Wang, Sai Wang Seto, Dennis Chang, Huazheng Liang
The present study aimed to test whether Hydroxysafflor yellow A (HSYA) protects the brain microvascular endothelial cells (BMECs) injury induced by oxygen glucose deprivation/reoxygenation (OGD/R) via the PI3K/Akt/mTOR autophagy signaling pathway. Primary rat BMECs were cultured and identified by the expression of factor VIII-related antigen before being exposed to OGD/R to imitate ischemia/reperfusion (I/R) damage in vitro. The protective effect of HSYA was evaluated by assessing (1) cellular morphologic and ultrastructural changes; (2) cell viability and cytotoxicity; (3) transendothelial electrical resistance (TEER) of monolayer BMECs; (4) cell apoptosis; (5) fluorescence intensity of LC3B; (6) LC3 mRNA expression; (7) protein expressions of LC3, Beclin-1, Zonula occludens-1 (ZO-1), phospho-Akt (p-Akt), Akt, phospho-mTOR (p-mTOR) and mTOR...
May 15, 2018: Brain Research Bulletin
https://www.readbyqxmd.com/read/29774638/loss-of-sirtuin-1-and-mitofusin-2-contributes-to-enhanced-ischemia-reperfusion-injury-in-aged-livers
#5
Sung Kook Chun, Sooyeon Lee, Joseph Flores-Toro, Rebecca Y U, Ming-Jim Yang, Kristina L Go, Thomas G Biel, Catherine E Miney, Schiley Pierre Louis, Brian K Law, Mary E Law, Elizabeth M Thomas, Kevin E Behrns, Christiaan Leeuwenburgh, Jae-Sung Kim
Ischemia/reperfusion (I/R) injury is a causative factor contributing to morbidity and mortality during liver resection and transplantation. Livers from elderly patients have a poorer recovery from these surgeries, indicating reduced reparative capacity with aging. Mechanisms underlying this age-mediated hypersensitivity to I/R injury remain poorly understood. Here, we investigated how sirtuin 1 (SIRT1) and mitofusin 2 (MFN2) are affected by I/R in aged livers. Young (3 months) and old (23-26 months) male C57/BL6 mice were subjected to hepatic I/R in vivo...
May 17, 2018: Aging Cell
https://www.readbyqxmd.com/read/29771603/negative-feedback-loop-of-autophagy-and-endoplasmic-reticulum-stress-in-rapamycin-protection-against-renal-ischemia-reperfusion-injury-during-initial-reperfusion-phase
#6
Xinyuan Li, Gongmin Zhu, Xin Gou, Weiyang He, Hubin Yin, Xiaoyu Yang, Jie Li
Rapamycin, an immunosuppressant, is widely used in patients with kidney transplant. However, the therapeutic effects of rapamycin remain controversial. Additionally, previous studies have revealed deleterious effects of rapamycin predominantly when administered for ≥24 h. Few studies, however, have focused on the short-term effects of rapamycin administered only during the initial reperfusion phase. As such, we designed this study to explore the potential effects and mechanisms of rapamycin under a specific therapeutic regimen in which rapamycin is mixed in the perfusate during the initial reperfusion phase (within 24 h)...
May 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29761825/zinc-mitigates-renal-ischemia-reperfusion-injury-in-rats-by-modulating-oxidative-stress-endoplasmic-reticulum-stress-and-autophagy
#7
Najet Hadj Abdallah, Anna Baulies, Ahlem Bouhlel, Mohamed Bejaoui, Mohamed A Zaouali, Safa Ben Mimouna, Imed Messaoudi, José C Fernandez-Checa, Carmen García Ruiz, Hassen Ben Abdennebi
Oxidative stress is a major factor involved in the pathogenesis of renal ischemia/reperfusion (I/R). Exogenous zinc (Zn) was suggested as a potent antioxidant; however, the mechanism by which it strengthens the organ resistance against the effects of reactive oxygen species (ROS) is not yet investigated. The present study aims to determine whether acute zinc chloride (ZnCl2 ) administration could attenuate endoplasmic reticulum (ER) stress, autophagy, and inflammation after renal I/R. Rats were subjected to either sham operation (Sham group, n = 6), or 1 hr of bilateral ischemia followed by 2 hr of reperfusion (I/R groups, n = 6), or they received ZnCl2 orally 24 hr and 30 min before ischemia (ZnCl2 group, n = 6)...
May 15, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29751053/l-cysteine-suppresses-hypoxia-ischemia-injury-in-neonatal-mice-by-reducing-glial-activation-promoting-autophagic-flux-and-mediating-synaptic-modification-via-h-2-s-formation
#8
Danqing Xin, Xili Chu, Xuemei Bai, Weiwei Ma, Hongtao Yuan, Jie Qiu, Changxing Liu, Tong Li, Xin Zhou, Wenqiang Chen, Dexiang Liu, Zhen Wang
We previously reported that L-Cysteine, an H2 S donor, significantly alleviated brain injury after hypoxia-ischemic (HI) injury in neonatal mice. However, the mechanisms underlying this neuroprotective effect of L-Cysteine against HI insult remain unknown. In the present study, we tested the hypothesis that the protective effects of L-Cysteine are associated with glial responses and autophagy, and L-Cysteine attenuates synaptic injury as well as behavioral deficits resulting from HI. Consistent with our previous findings, we found that treatment with L-Cysteine after HI reduced early brain injury, improved behavioral deficits and synaptic damage, effects which were associated with an up-regulation of synaptophysin and postsynaptic density protein 95 expression in the lesioned cortex...
May 8, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29750875/ulinastatin-alleviates-neurological-deficiencies-evoked-by-transient-cerebral-ischemia-via-improving-autophagy-nrf-2-are-and-apoptosis-signals-in-hippocampus
#9
Xiao-Ming Jiang, Jing-Hai Hu, Lu-Lu Wang, Chi Ma, Xu Wang, Xiao-Liang Liu
Ulinastatin [or called as urinary trypsin inhibitor (UTI)] plays a role in regulating neurological deficits evoked by transient cerebral ischemia. However, the underlying mechanisms still need to be determined. The present study was to examine the effects of UTI on autophagy, Nrf2-ARE and apoptosis signal pathway in the hippocampus in the process of neurological functions after cerebral ischemia using a rat model of cardiac arrest (CA). CA was induced by asphyxia followed by cardiopulmonary resuscitation (CPR) in rats...
May 10, 2018: Physiological Research
https://www.readbyqxmd.com/read/29748423/role-of-myeloid-lineage-cell-autophagy-in-ischemic-brain-injury
#10
Masakazu Kotoda, Hajime Furukawa, Takeshi Miyamoto, Masaaki Korai, Fumiaki Shikata, Atsushi Kuwabara, Xiaoxing Xiong, Caleb Rutledge, Rona G Giffard, Tomoki Hashimoto
BACKGROUND AND PURPOSE: Inflammatory cells play a significant role in secondary injury after ischemic stroke. Recent studies have suggested that a lack of autophagy in myeloid cells causes augmented proinflammatory cytokine release and prolonged inflammation after tissue injury. In this study, we investigated the roles of myeloid cell autophagy in ischemic brain injury. METHODS: Focal cerebral ischemia was induced via transient middle cerebral artery occlusion in mice with autophagy-deficient myeloid lineage cells (Atg5flox/flox LysMCre+) and in their littermate controls (Atg5flox/flox)...
May 10, 2018: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/29734780/cpkc%C3%AE-modulated-sequential-reactivation-of-mtor-inhibited-autophagic-flux-in-neurons-exposed-to-oxygen-glucose-deprivation-reperfusion
#11
Rongrong Hua, Song Han, Nan Zhang, Qingqing Dai, Ting Liu, Junfa Li
We have reported that conventional protein kinase Cγ (cPKCγ)-modulated neuron-specific autophagy improved the neurological outcome of mice following ischemic stroke through the Akt-mechanistic target of rapamycin (mTOR) pathway. However, its detailed molecular mechanism remains unclear. In this study, primary cortical neurons from postnatal one-day-old C57BL/6J cPKCγ wild-type ( cPKCγ +/+ ) and knockout ( cPKCγ −/− ) mice suffering oxygen glucose deprivation/reperfusion (OGD/R) were used to simulate ischemia/reperfusion injury in vitro...
May 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29734176/mitofusin-2-exerts-a-protective-role-in-ischemia-reperfusion-injury-through-increasing-autophagy
#12
Cheng Peng, Wei Rao, Lei Zhang, Fan Gao, Hao Hui, Kai Wang, Shuhui Dai, Yuefan Yang, Peng Luo, Yihui Ma, Wenke Ma, Xinguang Yu, Zhou Fei
BACKGROUND/AIMS: Autophagy is essential for maintaining cellular homeostasis and the survival of terminally differentiated cells as neurons. In this study, we aim to investigate whether mitofusin 2, a mitochondrial fusion protein, mediates autophagy in cerebral ischemia/reperfusion (I/R) injury. METHODS: Primary cultured neurons were treated with oxygen-glucose deprivation/reperfusion to mimic cerebral I/R injury in vitro. Autophagosomes were visualized upon TEM...
May 3, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29724989/role-of-hypoxia-inducible-factors-1%C3%AE-hif1%C3%AE-in-sh-sy5y-cell-autophagy-induced-by-oxygen-glucose-deprivation
#13
Guohui Niu, Dengna Zhu, Xiaoli Zhang, Jun Wang, Yunxia Zhao, Xin Wang
BACKGROUND HIF-1α plays an important role in hypoxia-ischemia brain damage. Accumulating evidences demonstrates that HIF-1α can contribute to cell autophagy. Oxygen-glucose deprivation (OGD) is a commonly used ischemic model in vitro. Our study was performed to investigate the influences of HIF-1α on autophagy in SH-SY5Y cells under OGD treatment. MATERIAL AND METHODS An OGD model was constructed in SH-SY5Y cells. PI method and MTT assay were used to test cell death and viability, respectively. Western blot assay was used to estimate the protein levels of HIF-1α and LC3...
May 4, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29722336/mitochondrial-autophagy-and-nlrp3-inflammasome-in-pulmonary-tissues-from-severe-combined-immunodeficient-mice-after-cardiac-arrest-and-cardiopulmonary-resuscitation
#14
Jing-Jun Lyu, Jawahar L Mehta, Yi Li, Lu Ye, Sheng-Nan Sun, Hong-Shuang Sun, Jia-Chang Li, Dong-Mei Zhang, Jie Wei
Background: The incidence of cancer, diabetes, and autoimmune diseases has been increasing. Furthermore, there are more and more patients with solid organ transplants. The survival rate of these immunocompromised individuals is extremely low when they are severely hit-on. In this study, we established cardiac arrest cardiopulmonary resuscitation (CPR) model in severe combined immunodeficient (SCID) mice, analyzed the expression and activation of mitochondrial autophagy and NLRP3 inflammasome/caspase-1, and explored mitochondrial repair and inflammatory injury in immunodeficiency individual during systemic ischemia-reperfusion injury...
May 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29706629/targeting-the-mir-665-3p-atg4b-autophagy-axis-relieves-inflammation-and-apoptosis-in-intestinal-ischemia-reperfusion
#15
Zhenlu Li, Guangzhi Wang, Dongcheng Feng, Guo Zu, Yang Li, Xue Shi, Yan Zhao, Huirong Jing, Shili Ning, Weidong Le, Jihong Yao, Xiaofeng Tian
Autophagy is an essential cytoprotective response against pathologic stresses that selectively degrades damaged cellular components. Impaired autophagy contributes to organ injury in multiple diseases, including ischemia/reperfusion (I/R), but the exact mechanism by which impaired autophagy is regulated remains unclear. Several researchers have demonstrated that microRNAs (miRNAs) negatively regulate autophagy by targeting autophagy-related genes (ATGs). Therefore, the effect of ATG-related miRNAs on I/R remains a promising research avenue...
April 30, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29704549/the-role-of-autophagy-in-acute-brain-injury-a-state-of-flux
#16
REVIEW
Michael S Wolf, Hülya Bayır, Patrick M Kochanek, Robert S B Clark
It is established that increased autophagy is readily detectable after various types of acute brain injury, including trauma, focal and global cerebral ischemia. What remains controversial, however, is whether this heightened detection of autophagy in brain represents a homeostatic or pathologic process, or an epiphenomenon. The ultimate role of autophagy after acute brain injury likely depends upon: (Galluzzi et al., 2016) the degree of brain injury and the overall autophagic burden; (Smith et al., 2011) the capacity of individual cell types to ramp up autophagic flux; (Mizushima et al...
April 25, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29704511/ngal-attenuates-renal-ischemia-reperfusion-injury-through-autophagy-activation-and-apoptosis-inhibition-in-rats
#17
Ya-Li Zhang, Shu-Kai Qiao, Rong-Ying Wang, Xiao-Nan Guo
Ischemia/reperfusion (I/R) injury is a main cause of acute kidney injury (AKI), and currently lacks effective therapies. This study is to investigate the level of Neutrophil gelatinase-associated lipocalin (NGAL) and autophagy status during renal I/R injury, so as to determine whether the exogenous NGAL protein could exert a protective effect for I/R injury and explore the potential mechanisms. Forty male Wistar rats were randomly divided into the following four groups: Sham, I/R, pre-treated with NGAL before I/R (I/R + pre-N), treated with NGAL after I/R (I/R + post-N)...
April 25, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29704468/renal-ischemia-reperfusion-induced-mitophagy-protects-against-renal-dysfunction-via-drp1-dependent-pathway
#18
Nan Li, Hengjin Wang, Chunming Jiang, Miao Zhang
Autophagy is upregulated under stress conditions to degrade superfluous proteins and recycle damaged organelles including damaged mitochondria. However, the occurrence of mitochondrial autophagy and its contribution remain to be elucidated during renal ischemia/reperfusion injury (IRI). In this study, mitophagosomes and engulfed mitochondria were frequently observed by electron microscopy after renal IRI vs. control. Meanwhile, the increase of lipidated microtubule associated protein light chain 3 (LC3-II) and decrease of mitochondrial proteins were detected by western blot, suggesting the presence of mitophagy...
April 25, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29701696/estrogen-and-or-estrogen-receptor-%C3%AE-inhibits-bnip3-induced-apoptosis-and-autophagy-in-h9c2-cardiomyoblast-cells
#19
Bih-Cheng Chen, Yi-Jiun Weng, Marthandam Asokan Shibu, Chien-Kuo Han, Yueh-Sheng Chen, Chia-Yao Shen, Yueh-Min Lin, Vijaya Padma Viswanadha, Hsin-Yueh Liang, Chih-Yang Huang
The process of autophagy in heart cells maintains homeostasis during cellular stress such as hypoxia by removing aggregated proteins and damaged organelles and thereby protects the heart during the times of starvation and ischemia. However, autophagy can lead to substantial cell death under certain circumstances. BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), a hypoxia-induced marker, has been shown to induce both autophagy and apoptosis. A BNIP3-docked organelle, e.g., mitochondria, also determines whether autophagy or apoptosis will take place...
April 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29701054/-statistics-and-analysis-on-acupuncture-and-moxibustion-projects-of-the-national-natural-science-foundation-of-china-of-traditional-chinese-medicine-universities-and-colleges-in-recent-10-years-taking-the-general-program-and-national-science-fund-for-young
#20
Qingling Li, Qiang Ma, Dan Li, Nana Liu, Jiahui Yang, Chun Sun, Cheng Cheng, Xuezhao Jia, Jing Wang, Yonglei Zeng
To analyze statistically the situation of the National Natural Science Foundation of China (NSFC) from 2007 to 2016 in the field of acupuncture and moxibustion for supporting the national Universities colleges of traditional Chinese medicine on the General Program (GP) and the National Science Fund for Young Scholars (NSFYS). In view of five aspects, named fund, supporting units, key words, method, disorder and signal path, the differences were compared between GP and NSFYS, the following characteristics were summarized...
March 12, 2018: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
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