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https://www.readbyqxmd.com/read/27862958/nicotine-induced-protein-expression-profiling-reveals-mutually-altered-proteins-across-four-human-cell-lines
#1
Joao A Paulo, Steven P Gygi
Mass spectrometry-based proteomic strategies can profile the expression level of proteins in response to external stimuli. Nicotine affects diverse cellular pathways, however, the nicotine-induced alterations on the global proteome across human cell lines have not been fully elucidated. We measured perturbations in protein levels resulting from nicotine treatment in four cell lines - HEK, HeLa, PaSC, and SH-SY5Y- in a single experiment using tandem mass tags (TMT10-plex) and high-resolution mass spectrometry...
November 12, 2016: Proteomics
https://www.readbyqxmd.com/read/27711123/language-agnostic-reproducible-data-analysis-using-literate-programming
#2
Boris Vassilev, Riku Louhimo, Elina Ikonen, Sampsa Hautaniemi
A modern biomedical research project can easily contain hundreds of analysis steps and lack of reproducibility of the analyses has been recognized as a severe issue. While thorough documentation enables reproducibility, the number of analysis programs used can be so large that in reality reproducibility cannot be easily achieved. Literate programming is an approach to present computer programs to human readers. The code is rearranged to follow the logic of the program, and to explain that logic in a natural language...
2016: PloS One
https://www.readbyqxmd.com/read/27542271/targeting-a-novel-cancer-driving-protein-laptm4b-35-by-a-small-molecule-ets-to-inhibit-cancer-growth-and-metastasis
#3
Maojin Li, Rouli Zhou, Yi Shan, Li Li, Lin Wang, Gang Liu
: Our previous studies demonstrated that LAPTM4B-35 is overexpressed in a variety of solid cancers including hepatocellular carcinoma (HCC), and is an independent factor for prognosis. LAPTM4B-35 overexpression causes carcinogenesis and enhances cancer growth, metastasis and multidrug resistance, and thus may be a candidate for therapeutic targeting. The present study shows ethylglyoxal bisthiosemicarbazon (ETS) has effective anticancer activity through LAPTM4B-35 targeting. Bel-7402 and HepG2 cell lines from human HCC were used as cell models in which LAPTM4B-35 is highly expressed, and a human fetal liver cell line was used as a control...
August 17, 2016: Oncotarget
https://www.readbyqxmd.com/read/27486880/overexpressed-laptm4b-35-is-a-risk-factor-for-cancer-recurrence-and-poor-prognosis-in-non-small-cell-lung-cancer
#4
Fanming Kong, Fangfang Gao, Jun Chen, Yiyu Sun, Ying Zhang, Honggen Liu, Xiaojiang Li, PeiYing Yang, Rongxiu Zheng, Geli Liu, Yingjie Jia
BACKGROUND: The expression levels and clinical significances of Lysosomal-associated protein transmembrane-4β-35 (LAPTM4B-35) protein are unknown in the non-small-cell lung cancer (NSCLC). This study aimed to explore the expression and prognostic value of LAPTM4B-35 in NSCLC patients. METHODS: The clinicopathological and survival data of 107 NSCLC patients who received radical surgery from 2007 and 2011 were reviewed. The LAPTM4B-35 expression of the paired tumors and adjacent normal specimens were detected, and the association between LAPTM4B-35 and clinical variables was explored...
July 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27391361/relationship-between-laptm4b-gene-polymorphism-and-prognosis-of-patients-following-tumor-resection-for-colorectal-and-esophageal-cancers
#5
Xiaojing Cheng, Xiuyun Tian, Xiaojiang Wu, Xiaofang Xing, Hong Du, Chunlian Zhou, Qingyun Zhang, Chunyi Hao, Xianzi Wen, Jiafu Ji
BACKGROUND: Lysosome-associated transmembrane-4 beta (LAPTM4B) is an oncogene that participates tumorgenesis in a variety of human solid tumors, and it has two alleles named as LAPTM4B*1 and *2. The present study aimed to identify the association of LAPTM4B genotype with clinicopathological features and prognosis in colorectal and esophageal cancer patients. METHOD: Genotypes of LAPTM4B were determined by PCR in 167 colon cancer cases (72 patients in a discovery cohort and 95 patients in a testing cohort), 160 rectal cancer cases and 164 esophageal cancer cases...
2016: PloS One
https://www.readbyqxmd.com/read/27212036/laptm4b-an-oncogene-in-various-solid-tumors-and-its-functions
#6
Y Meng, L Wang, D Chen, Y Chang, M Zhang, J-J Xu, R Zhou, Q-Y Zhang
The oncogene Lysosome-associated protein transmembrane-4β (LAPTM4B) gene was identified, and the polymorphism region in the 5'-UTR of this gene was certified to be associated with tumor susceptibility. LAPTM4B-35 protein was found to be highly expressed in various solid tumors and could be a poor prognosis marker. The functions of LAPTM4B in solid tumors were also explored. It is suggested that LAPTM4B could promote the proliferation of tumor cells, boost invasion and metastasis, resist apoptosis, initiate autophagy and assist drug resistance...
May 23, 2016: Oncogene
https://www.readbyqxmd.com/read/26984064/far-red-near-infrared-fluorescence-light-up-probes-for-specific-in-vitro-and-in-vivo-imaging-of-a-tumour-related-protein
#7
Chao Chen, Yongquan Hua, Yawen Hu, Yuan Fang, Shenglu Ji, Zhimou Yang, Caiwen Ou, Deling Kong, Dan Ding
As lysosomal protein transmembrane 4 beta (LAPTM4B) is an important biomarker for many solid tumours, development of small-molecule fluorescence light-up probes for detection and imaging of LAPTM4B proteins is particularly valuable. In this work, we reported the design and synthesis of a far-red/near-infrared (FR/NIR) fluorescence light-up probe DBT-2EEGIHGHHIISVG, which could specifically visualize LAPTM4B proteins in cancer cells and tumour-bearing live mice. DBT-2EEGIHGHHIISVG was synthesized by the conjugation of two LAPTM4B-binding peptide ligands (EEGIHGHHIISVG) with one environment-sensitive fluorogen, 4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole (DBT)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26526574/predictive-significance-of-combined-laptm4b-and-vegf-expression-in-patients-with-cervical-cancer
#8
Fanling Meng, Shu Tan, Tianbo Liu, Hongtao Song, Ge Lou
Lysosome-associated protein transmembrane 4ß-35 (LAPTM4B-35) is overexpressed in several solid malignancies. This study determines the expression level of LAPTM4B-35 in the cervical cancer during tumor development and progression. The present study investigated the clinicopathological significance of the coexpression of LAPTM4B-35 and VEGF in patients with cervical cancer. Immunohistochemistry was used to evaluate the expression of LAPTM4B-35 and VEGF in 62 cervical intraepithelial neoplasia (CIN) and 226 cervical carcinoma in comparison with 45 normal cervical specimens...
April 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/26383850/laptm4b-down-regulation-inhibits-the-proliferation-invasion-and-angiogenesis-of-hela-cells-in-vitro
#9
Fanling Meng, Xiuwei Chen, Hongtao Song, Ge Lou
BACKGROUND/AIMS: LAPTM4B (lysosome-associated protein transmembrane 4 beta) is a novel oncogene with important functions in aggressive human carcinomas, including cervical cancer. However, the specific functions and internal molecular mechanisms associated with this gene in the context of cervical cancer remain unclear. METHODS: In this study, we explored the effects and mechanisms of LAPTM4B on tumor growth, metastasis and angiogenesis in vitro by depletion of LAPTM4B in Hela cell...
2015: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/26343532/laptm4b-is-associated-with-poor-prognosis-in-nsclc-and-promotes-the-nrf2-mediated-stress-response-pathway-in-lung-cancer-cells
#10
Yuho Maki, Junya Fujimoto, Wenhua Lang, Li Xu, Carmen Behrens, Ignacio I Wistuba, Humam Kadara
We recently demonstrated that lysosomal protein transmembrane 4 beta (LAPTM4B) is elevated in non-small cell lung cancers (NSCLCs) and in the surrounding premalignant airway field of cancerization. In the present study, we sought to begin to understand the relevance of LAPTM4B expression and signaling to NSCLC pathogenesis. In situ hybridization analysis of LAPTM4B transcript in tissue microarrays comprised of 368 NSCLCs demonstrated that LAPTM4B expression was significantly increased in smoker compared to non-smoker lung adenocarcinoma tumors (P < 0...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26280656/laptm4b-facilitates-late-endosomal-ceramide-export-to-control-cell-death-pathways
#11
Tomas Blom, Shiqian Li, Andrea Dichlberger, Nils Bäck, Young Ah Kim, Ursula Loizides-Mangold, Howard Riezman, Robert Bittman, Elina Ikonen
Lysosome-associated protein transmembrane-4b (LAPTM4B) associates with poor prognosis in several cancers, but its physiological function is not well understood. Here we use novel ceramide probes to provide evidence that LAPTM4B interacts with ceramide and facilitates its removal from late endosomal organelles (LEs). This lowers LE ceramide in parallel with and independent of acid ceramidase-dependent catabolism. In LAPTM4B-silenced cells, LE sphingolipid accumulation is accompanied by lysosomal membrane destabilization...
October 2015: Nature Chemical Biology
https://www.readbyqxmd.com/read/26247403/lentivirus-mediated-rna-interference-targeting-laptm4b-inhibits-human-ovarian-cancer-cell-invasion-in-vitro
#12
Fanling Meng, Xiuwei Chen, Hongtao Song, Ge Lou, Songbin Fu
LAPTM4B (lysosome-associated protein transmembrane 4 beta) play an important role in several human carcinomas. We examines the effects of RNA interference mediated downregulation of human lysosomal-associated protein transmembrane 4 beta expression on the biological behavior of the human serous adenocarcinoma cell line NIH:OVCAR3. This study investigated the expression level of lysosomal-associated protein transmembrane 4 beta in several ovarian cancer cell lines. RNA interference mediated by recombinant lentiviral vectors expressing an artificial lysosomal-associated protein transmembrane 4 beta miRNA was used to induce long-lasting downregulation of lysosomal-associated protein transmembrane 4 beta gene expression in NIH:OVCAR3 cells...
January 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/26126825/lysosomal-associated-transmembrane-protein-4b-laptm4b-decreases-transforming-growth-factor-%C3%AE-1-tgf-%C3%AE-1-production-in-human-regulatory-t-cells
#13
Caroline Huygens, Stéphanie Liénart, Olivier Dedobbeleer, Julie Stockis, Emilie Gauthy, Pierre G Coulie, Sophie Lucas
Production of active TGF-β1 is one mechanism by which human regulatory T cells (Tregs) suppress immune responses. This production is regulated by glycoprotein A repetitions predominant (GARP), a transmembrane protein present on stimulated Tregs but not on other T lymphocytes (Th and CTLs). GARP forms disulfide bonds with proTGF-β1, favors its cleavage into latent inactive TGF-β1, induces the secretion and surface presentation of GARP·latent TGF-β1 complexes, and is required for activation of the cytokine in Tregs...
August 14, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26108779/-impact-of-lysosome-associated-protein-transmembrane-4-beta-on-proliferation-and-invasion-of-colorectal-cancer
#14
Yang Luo, Zhengqian Bian, Guangyao Ye, Minhao Yu, Zhengshi Wang, Shaolan Qin, Yifei Mu, Ming Zhong
OBJECTIVE: To determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC). METHODS: Thirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues...
June 2015: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/26063982/lysosome-and-cytoskeleton-pathways-are-robustly-enriched-in-the-blood-of-septic-patients-a-meta-analysis-of-transcriptomic-data
#15
REVIEW
Jie Ma, Chuanxi Chen, Andreas S Barth, Chris Cheadle, Xiangdong Guan, Li Gao
BACKGROUND: Sepsis is a leading cause of mortality in intensive care units worldwide. A better understanding of the blood systems response to sepsis should expedite the identification of biomarkers for early diagnosis and therapeutic interventions. METHODS: We analyzed microarray studies whose data is available from the GEO repository and which were performed on the whole blood of septic patients and normal controls. RESULTS: We identified 6 cohorts consisting of 450 individuals (sepsis = 323, control = 127) providing genome-wide messenger RNA (mRNA) expression data...
2015: Mediators of Inflammation
https://www.readbyqxmd.com/read/25998567/laptm4b-recruits-the-lat1-4f2hc-leu-transporter-to-lysosomes-and-promotes-mtorc1-activation
#16
Ruth Milkereit, Avinash Persaud, Liviu Vanoaica, Adriano Guetg, Francois Verrey, Daniela Rotin
Mammalian target of rapamycin 1 (mTORC1), a master regulator of cellular growth, is activated downstream of growth factors, energy signalling and intracellular essential amino acids (EAAs) such as Leu. mTORC1 activation occurs at the lysosomal membrane, and involves V-ATPase stimulation by intra-lysosomal EAA (inside-out activation), leading to activation of the Ragulator, RagA/B-GTP and mTORC1 via Rheb-GTP. How Leu enters the lysosomes is unknown. Here we identified the lysosomal protein LAPTM4b as a binding partner for the Leu transporter, LAT1-4F2hc (SLC7A5-SLAC3A2)...
2015: Nature Communications
https://www.readbyqxmd.com/read/25932367/the-relationship-between-laptm4b-polymorphisms-and-cancer-risk-in-chinese-han-population-a-meta-analysis
#17
Ling-Zi Xia, Zhi-Hua Yin, Yang-Wu Ren, Li Shen, Wei Wu, Xue-Lian Li, Peng Guan, Bao-Sen Zhou
LAPTM4B is a newly cloned gene that shows an active role in many solid tumors progression in substantial researches, mainly through the autophage function. Accumulated studies have been conducted to determine the association of LAPTM4B polymorphism with cancer risk. While the results are inconsistent, we conducted the meta-analysis to determine the strength of the relationship. Results showed that allele*2 carriers exhibited a significantly increased risk of cancer development with comparison to allele*1 homozygote (for *1/2, OR = 1...
2015: SpringerPlus
https://www.readbyqxmd.com/read/25910706/lysosome-associated-protein-transmembrane-4%C3%AE-35-is-a-novel-independent-prognostic-factor-in-small-cell-lung-cancer
#18
COMPARATIVE STUDY
Man Qiao, Guohua Hu
The lysosome-associated protein transmembrane-4β-35 (LAPTM4B-35) protein has been indicated to be involved in solid tumors, while its role in small cell lung cancer (SCLC) remains unknown. The aim of this study is to investigate the LAPTM4B-35 protein expression and its clinical and prognostic role in SCLC patients. A total of 88 SCLC patients who underwent radical surgery between 2002 and 2010 were enrolled in the study. The level of messenger RNA (mRNA) and protein was detected from the fresh paired tumor specimens and adjacent normal tissues...
September 2015: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/25881887/differential-expression-of-lysosome-associated-protein-transmembrane-4-beta-laptm4b-in-granulosa-cells-of-ovarian-follicles-and-in-other-bovine-tissues
#19
Kalidou Ndiaye, Paul D Carrière, Jean Sirois, David W Silversides, Jacques G Lussier
BACKGROUND: LAPTM4B is a member of the lysosome-associated transmembrane protein superfamily that is differentially expressed in normal human tissues and upregulated in various types of carcinomas. These proteins are thought to be involved in the regulation of cell proliferation and survival. The objective of this study was to investigate the expression of bovine LAPTM4B during ovarian follicular development and in various bovine tissues. METHODS AND RESULTS: Northern blot analysis revealed a 1...
2015: Journal of Ovarian Research
https://www.readbyqxmd.com/read/25849595/laptm4b-35-a-cancer-related-gene-is-associated-with-poor-prognosis-in-tnm-stages-i-iii-gastric-cancer-patients
#20
Xiaojing Cheng, Zhixue Zheng, Zhaode Bu, Xiaojiang Wu, Lianhai Zhang, Xiaofang Xing, Xiaohong Wang, Ying Hu, Hong Du, Lin Li, Shen Li, Rouli Zhou, Xian-Zi Wen, Jia-Fu Ji
BACKGROUND: Lysosome-associated transmembrane protein 4β-35 (LAPTM4B-35), a member of the mammalian 4-tetratransmembrane spanning protein superfamily, has been reported to be overexpressed in several cancers. However the expression of LAPTM4B-35 and its role in the progression of gastric cancer (GC) remains unknown. The aim of this study was to investigate LAPTM4B-35 expression in GC, its potential relevance to clinicopathologic parameters and role of LAPTM4B-35 during gastric carcinogenesis...
2015: PloS One
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