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Toxocara canis

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https://www.readbyqxmd.com/read/27901644/clinical-profile-treatment-and-visual-outcome-of-ocular-toxocara-in-a-tertiary-eye-care-centre
#1
Ekta S Sahu, Bikramjit Pal, Tarun Sharma, Jyotirmay Biswas
PURPOSE: To determine the clinical features, treatment, and visual outcome in ocular toxocariasis. METHODS: A total of 16 cases diagnosed as ocular toxocariasis clinically and/or serologically were analyzed retrospectively. RESULTS: The mean age of the patients was 22.6 years. Cases were categorized into three clinical types with peripheral granuloma (43.7%) as the most common presentation. Of the patients, 10 (62.5%) had positive serum ELISA for T...
November 30, 2016: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/27893948/seroprevalence-and-risk-factors-for-toxocara-canis-infection-in-serbia-during-2015
#2
Simona Gabrielli, Suzana Tasić-Otašević, Aleksandra Ignjatović, Maurizio Fraulo, Marija Trenkić-Božinović, Stefan Momčilović, Gabriella Cancrini
We report data on the Toxocara seroprevalence evidenced in 2015 from samples of 40 children and 298 adults of the population living in different areas of Serbia, and on possible association of certain variables with infection. Detection of specific antibodies was performed using an enzyme-linked immunosorbent assay; all ambiguous results and part of the positive and negative sera were further analyzed by confirmatory Western blot test. An overall 23.5% seroprevalence was noticed, which was confirmed in 13.0% of the examined population with no significant difference regarding the age (children = 10...
November 28, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27874323/flotation-of-toxocara-canis-eggs-in-commercial-bleach-and-effects-of-bleach-treatment-times-on-larval-development-in-these-eggs
#3
Alexa C Rosypal, Alice Houk-Miles, Anne M Zajac, David S Lindsay
Toxocara canis is a common intestinal nematode of young dogs. Puppies contaminate the environment with large numbers of eggs that can embryonate and become infective in less than a month. Embryonated eggs are infectious for humans and other paratenic hosts. Most T. canis infections in humans are asymptomatic, however, migration of T. canis larvae in the eye and in the central nervous system can result in vision loss, blindness, and even death. The eggs of T. canis are highly resistant to harsh environmental conditions and routinely used chemical disinfectants...
November 22, 2016: Journal of Parasitology
https://www.readbyqxmd.com/read/27857820/detection-of-specific-antibody-reactivity-to-toxocara-larval-excretory-secretory-antigens-in-asthmatic-patients-5-15-years
#4
Mahdi Mosayebi, Latif Moini, Reza Hajihossein, Mojtaba Didehdar, Zahra Eslamirad
BACKGROUND & PURPOSE: Humans act as an intermediate host for Toxocara canis and Toxocara cati. Toxocara may be an important risk factor for asthma in humans. The aim of the present study was to evaluate immunoglobulin G (IgG) anti-Toxocara canis antibody, using enzyme-linked immunosorbent assay (ELISA) in asthmatic patients (aged 5-15 years), referring to a clinic of pulmonary diseases in Arak, Iran. MATERIALS & METHODS: In this bi-group cross sectional study, serum samples were collected from 110 children with confirmed asthma and 70 children without asthma within one year...
2016: Open Microbiology Journal
https://www.readbyqxmd.com/read/27845205/comparative-transcriptomic-analyses-of-male-and-female-adult-toxocara-canis
#5
Rong-Qiong Zhou, Guang-Xu Ma, Pasi K Korhonen, Yong-Li Luo, Hong-Hong Zhu, Yong-Fang Luo, Robin B Gasser, Qing-You Xia
Toxocariasis is an important, neglected zoonosis caused mainly by Toxocara canis. Although our knowledge of helminth molecular biology is improving through completed draft genome projects, there is limited detailed information on the molecular biology of Toxocara species. Here, transcriptomic sequencing of male and female adult T. canis and comparative analyses were conducted. For each sex, two-thirds (66-67%) of quality-filtered reads mapped to the gene set of T. canis, and at least five reads mapped to each of 16,196 (87...
November 11, 2016: Gene
https://www.readbyqxmd.com/read/27800525/staphylococcus-aureus-endocarditis-as-a-complication-of-toxocariasis-associated-endomyocarditis-with-fibrosis-a-case-report
#6
Esther Kuenzli, Niklaus Labhardt, Gianmarco Balestra, Maja Weisser, Michael J Zellweger, Johannes Blum
Complications associated with Toxocara canis infection are rare. We present a case of a patient with Staphylococcus aureus endocarditis as a complication of an endomyocardial fibrosis caused by T canis. The epidemiological, pathological, and clinical features of this rare complication are described here.
September 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/27794395/arginine-kinase-in-toxocara-canis-exon-intron-organization-functional-analysis-of-site-directed-mutants-and-evaluation-of-putative-enzyme-inhibitors
#7
Susiji Wickramasinghe, Lalani Yatawara, Mitsuru Nagataki, Takeshi Agatsuma
OBJECTIVES: To determine exon/intron organization of the Toxocara canis (T. canis) AK (TCAK) and to test green and black tea and several other chemicals against the activity of recombinant TCAK in the guanidino-specific region by site-directed mutants. METHODS: Amplification of genomic DNA fragments containing introns was carried out by PCRs. The open-reading frame (1200 bp) of TCAK (wild type) was cloned into the BamH1/SalI site of pMAL-c2X. The maltose-binding protein-TCAK fusion protein was expressed in Escherichia coli TB1 cells...
October 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27778108/seroprevalence-of-five-parasitic-pathogens-in-pregnant-women-in-ten-caribbean-countries
#8
Fengguang Guo, Martin S Forde, Stephen R Werre, Rosina C Krecek, Guan Zhu
To date, published epidemiological studies of parasitic infections in humans in the Caribbean region are very limited. Here, we report the seroprevalence of five parasitic pathogens, including Ascaris lumbricoides, Entamoeba histolytica, Giardia lamblia, Schistosoma mansoni, and Toxocara canis in 435 serum samples collected between 2008 and 2011 from pregnant women in ten Caribbean islands. We tested the serum samples for IgG antibodies against the five parasites by enzyme-linked immunosorbent assay (ELISA)...
October 24, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27760094/-ocular-toxocariasis-in-a-boy-with-idiopathic-nephrotic-syndrome-a-case-report
#9
Agnieszka Antonowicz, Piotr Skrzypczyk, Beata Kępa, Małgorzata Pańczyk-Tomaszewska
: Toxocariasis is a common zoonosis caused by infection with Toxocara canis or cati larvae. Ocular toxocariasis is one of the forms of infestation found in 1/1,000 - 1/10,000 children. Children with idiopathic nephrotic syndrome (INS) are at high risk of infections, also parasitic infestations, which can, in turn, cause relapses of the disease. A CASE REPORT: We present a case of a 6-year-old boy with steroiddependent nephrotic syndrome. The disease started at age of 2, the boy had 9 relapses of INS, and was treated with oral prednisone, levamisole, and cyclophosphamide...
October 19, 2016: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/27746165/kinetic-and-avidity-of-igy-anti-toxocara-antibodies-in-experimentally-infected-chickens
#10
Ricardo da Silva Raposo, Vamilton Alvares Santarém, Yslla Fernanda Fitz Balo Merigueti, Guita Rubinsky-Elefant, Letícia Maria de Lima Cerazo, Ludimilla Pereira, Bianca Pelegi Zampieri, Aristeu Vieira da Silva, Cecília Braga Laposy
Toxocariasis is a geohelminth zoonosis with worldwide distribution, mainly transmitted through the ingestion of embryonated eggs of nematodes of the Toxocara genus. The disease can also be transmitted to humans as a result of eating raw or undercooked meat of paratenic hosts, such as chickens. Here, we standardized an enzyme-linked immunosorbent assay (ELISA) for evaluating experimentally the kinetic and avidity index (AI) of IgY in broiler chickens infected with different doses of Toxocara canis eggs (G1:100; G2: 1000; and G3: 5000; n = 12 per group)...
October 13, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27733798/gastrointestinal-helminths-of-coyotes-canis-latrans-from-southeast-nebraska-and-shenandoah-area-of-iowa
#11
Whitni K Redman, Jay E Bryant, Gul Ahmad
AIM: This survey was carried out on the carcasses of 29 coyotes from Southeastern Nebraska and Shenandoah area of Iowa to document the helminths present in the intestinal track of these carnivorous animals. MATERIALS AND METHODS: A total of 29 adult coyote carcasses were generously donated in the autumn and winter (November-February) of 2014-2015 by trappers, fur buyers and hunters of Southeast Nebraska and Shenandoah area of Iowa. The intestine of individual animals were examined for the recovery of helminth parasites as per the established procedures...
September 2016: Veterinary World
https://www.readbyqxmd.com/read/27723790/serological-evidence-of-exposure-to-globally-relevant-zoonotic-parasites-in-the-estonian-population
#12
Brian Lassen, Marilin Janson, Arvo Viltrop, Kädi Neare, Pirje Hütt, Irina Golovljova, Lea Tummeleht, Pikka Jokelainen
We investigated Estonian population and its selected subgroups for serological evidence of exposure to Ascaris lumbricoides, Echinococcus spp., Taenia solium, Toxocara canis, Toxoplasma gondii, and Trichinella spiralis. Serum samples from 999 adults representing general population, 248 children aged 14-18, 158 veterinarians, 375 animal caretakers, and 144 hunters were tested for specific immunoglobulin G antibodies against the selected parasites using commercial enzyme immunoassays (ELISA). Sera yielding positive or twice grey zone Echinococcus spp, T...
2016: PloS One
https://www.readbyqxmd.com/read/27716389/recurrent-patent-infections-with-toxocara-canis-in-household-dogs-older-than-six-months-a-prospective-study
#13
Rolf Nijsse, Lapo Mughini-Gras, Jaap A Wagenaar, Harm W Ploeger
BACKGROUND: To reduce environmental contamination with Toxocara canis eggs, the current general advice is to deworm all dogs older than six months on average four times a year. However, only a small proportion of non-juvenile household dogs actually shed T. canis eggs, and some dogs shed eggs more frequently than others. The identification of these frequent shedders and the associated risk factors is an important cornerstone for constructing evidence-based deworming regimens. The purpose of this study is to identify risk factors associated with recurrence of periods of shedding Toxocara eggs in a cohort of household dogs older than six months...
October 4, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27698276/human-toxocariasis-prevalence-and-factors-associated-with-biosafety-in-research-laboratories
#14
Gabriela Torres Mattos, Paula Costa Dos Santos, Paula de Lima Telmo, Maria Elisabeth Aires Berne, Carlos James Scaini
Human toxocariasis is a neglected parasitic disease worldwide. Researchers studying this disease use infectious strains of Toxocara for experiments. Health workers are at risk in the course of their daily routine and must adhere to biosafety standards while carrying out the activities. Researchers on biosafety concerning working with these parasites are insufficient. The aim of this study was to determine the rate of seroprevalence of Toxocara species among health-care research laboratory workers (professors, technicians, and students), and to investigate the risk factors of Toxocara infection associated with laboratory practices...
October 3, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27688120/characterisation-of-a-new-highly-effective-method-for-detecting-nematode-eggs-ascaris-spp-toxocara-spp-trichuris-spp-in-sewage-sludge-containing-flocculants
#15
Jolanta Zdybel, Jacek Karamon, Mirosław Różycki, Ewa Bilska-Zając, Teresa Kłapeć, Tomasz Cencek
Because traditional methods used for sewage sludge parasitological examinations have low sensitivity, a new, highly effective method (own method - OM) was devised. The principle of this method is to eliminate the flocculent effect on the structure of sewage sludge by mechanically damaging floccules in the presence of surfactants and to increase the effectiveness of egg isolation processes in large volumes of liquids. The objective of this study was to estimate the effectiveness of the OM in detecting nematode eggs in sewage sludge samples containing flocculants...
September 28, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27680167/frequency-of-anti-toxocara-spp-antibodies-in-individuals-attended-by-the-centro-de-salud-familiar-and-environmental-contamination-with-toxocara-canis-eggs-in-dog-feces-in-the-coastal-niebla-town-chile
#16
Catalina Vargas, Patricio Torres, María Isabel Jercic, Marta Lobos, Alan Oyarce, Juan Carlos Miranda, Salvador Ayala
The frequency of anti-Toxocara spp. antibodies in individuals attended by the Centro de Salud Familiar in the coastal Niebla town, Chile, was related to the host and to environmental factors. IgG anti- Toxocara antibodies were detected with a commercial ELISA kit (SCIMEDX Corporation, USA). Samples with undetermined absorbance values were subjected to an additional ELISA standardized by the Instituto de Salud Pública, Chilean Health Ministry, a commercial ELISA (NOVATEC, Germany), and a commercial Western blot kit (LDBio Diagnostics, France)...
2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27638150/proteomic-analysis-of-toxocara-canis-excretory-and-secretory-tes-proteins
#17
Rita Leal Sperotto, Frederico Schmitt Kremer, Maria Elisabeth Aires Berne, Luciana F Costa de Avila, Luciano da Silva Pinto, Karina Mariante Monteiro, Karin Silva Caumo, Henrique Bunselmeyer Ferreira, Natália Berne, Sibele Borsuk
Toxocariasis is a neglected disease, and its main etiological agent is the nematode Toxocara canis. Serological diagnosis is performed by an enzyme-linked immunosorbent assay using T. canis excretory and secretory (TES) antigens produced by in vitro cultivation of larvae. Identification of TES proteins can be useful for the development of new diagnostic strategies since few TES components have been described so far. Herein, we report the results obtained by proteomic analysis of TES proteins using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach...
September 13, 2016: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/27622437/production-and-evaluation-of-the-recombinant-antigen-tes-30-of-toxocara-canis-for-the-immunodiagnosis-of-toxocariasis
#18
Ana M Olave, Jairo A Mesa, Jorge H Botero, Edwin B Patiño, Gisela M García, Juan F Alzate
INTRODUCTION: Toxocara canis is a pathogenic nematode of canines which can be accidentally transmitted to humans. Although serology is the most important diagnostic tool for this zoonosis, diagnostic kits use crude excretion/secretion antigens, most of them being glycoproteins which are not species-specific and may cross-react with antibodies generated against other parasites.  OBJECTIVES: To produce the rTES-30 recombinant antigen of Toxocara canis and evaluate it in the immunodiagnosis of toxocariasis...
2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27605834/in-vitro-production-of-toxocara-canis-excretory-secretory-tes-antigen
#19
Divyamol Thomas, N Jeyathilakan, S Abdul Basith, T M A Senthilkumar
Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27605812/domestic-dog-as-a-human-health-hazard-in-north-of-iran
#20
Shahabeddin Sarvi, Ahmad Daryani, Mahdi Sharif, Mohammad Taghi Rahimi, Davood Azami, Zahra Marhaba, Ehsan Ahmadpour, Azadeh Mizani
An epidemiological study was conducted to determine the prevalence of helminthic parasites of domestic dogs using stool samples in north of Iran in 2013. Stool samples (n = 100) were collected from domestic dogs of different ages and genders. Different techniques including wet smear, formalin-ether sedimentation method and flotation technique in saturated zinc chloride solution were performed on collected stools to detect eggs and larval stage of helminthes parasites. The overall prevalence of helminthic parasites of examined stools of dogs was 57 %...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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