Nienke Besbrugge, Jelle Van Leene, Dominique Eeckhout, Bernard Cannoot, Shubhada R Kulkarni, Nancy De Winne, Geert Persiau, Eveline Van De Slijke, Michiel Bontinck, Stijn Aesaert, Francis Impens, Kris Gevaert, Daniel van Damme, Mieke Van Lijsebettens, Dirk Inze, Klaas Vandepoele, Hilde Nelissen, Geert De Jaeger
The ability to tag proteins has boosted the emergence of generic molecular methods for protein functional analysis. Fluorescent protein tags are used to visualize protein localization and affinity tags enable the mapping of molecular interactions by, for example, tandem affinity purification (TAP) or chromatin immunoprecipitation (ChIP). To apply these widely used molecular techniques on a single transgenic plant line, we developed a multifunctional TAP tag, named GSyellow, which combines the streptavidin-binding peptide (SBP) tag with citrine yellow fluorescent protein (cYFP)...
April 20, 2018: Plant Physiology