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https://www.readbyqxmd.com/read/28220049/epigenetics-maternal-trim28-for-male-embryos
#1
Eytan Zlotorynski
No abstract text is available yet for this article.
February 21, 2017: Nature Reviews. Molecular Cell Biology
https://www.readbyqxmd.com/read/28159803/tripartite-motif-containing-28-bridges-endothelial-inflammation-and-angiogenic-activity-by-retaining-expression-of-tnfr-1-and-2-and-vegfr2-in-endothelial-cells
#2
Yinfang Wang, Jinping Li, Yitong Huang, Xiuqin Dai, Youbin Liu, Zongjun Liu, Ying Wang, Nanping Wang, Peng Zhang
Angiogenesis and inflammation are regarded as important factors in the pathogenesis of chronic inflammation, cancer, and wound healing. Recent studies have supported prior evidence that common signaling pathways are involved in angiogenesis and inflammatory responses; however, key factors controlling both processes remain unclear. Although tripartite motif-containing (TRIM)-28 is known to have an immunosuppressive role in immune cells, its expression level and role in endothelial cells (ECs) are still unclear...
February 3, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28134929/the-evolutionary-capacitor-hsp90-buffers-the-regulatory-effects-of-mammalian-endogenous-retroviruses
#3
Barbara Hummel, Erik C Hansen, Aneliya Yoveva, Fernando Aprile-Garcia, Rebecca Hussong, Ritwick Sawarkar
Understanding how genotypes are linked to phenotypes is important in biomedical and evolutionary studies. The chaperone heat-shock protein 90 (HSP90) buffers genetic variation by stabilizing proteins with variant sequences, thereby uncoupling phenotypes from genotypes. Here we report an unexpected role of HSP90 in buffering cis-regulatory variation affecting gene expression. By using the tripartite-motif-containing 28 (TRIM28; also known as KAP1)-mediated epigenetic pathway, HSP90 represses the regulatory influence of endogenous retroviruses (ERVs) on neighboring genes that are critical for mouse development...
January 30, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28115710/differential-control-of-retrovirus-silencing-in-embryonic-cells-by-proteasomal-regulation-of-the-zfp809-retroviral-repressor
#4
Cheng Wang, Stephen P Goff
Replication of the murine leukemia viruses is strongly suppressed in mouse embryonic stem (ES) cells. Proviral DNAs are formed normally but are then silenced by a large complex bound to DNA by the ES cell-specific zinc-finger protein ZFP809. We show here that ZFP809 expression is not regulated by transcription but rather by protein turnover: ZFP809 protein is stable in embryonic cells but highly unstable in differentiated cells. The protein is heavily modified by the accumulation of polyubiquitin chains in differentiated cells and stabilized by the proteasome inhibitor MG132...
February 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28115466/loss-of-maternal-trim28-causes-male-predominant-early-embryonic-lethality
#5
Abhishek Sampath Kumar, Michelle K Y Seah, Ka Yi Ling, Yaju Wang, Joel H L Tan, Sandra Nitsch, Shu Ly Lim, Chanchao Lorthongpanich, Heike Wollmann, Diana H P Low, Ernesto Guccione, Daniel M Messerschmidt
Global DNA demethylation is a hallmark of embryonic epigenetic reprogramming. However, embryos engage noncanonical DNA methylation maintenance mechanisms to ensure inheritance of exceptional epigenetic germline features to the soma. Besides the paradigmatic genomic imprints, these exceptions remain ill-defined, and the mechanisms ensuring demethylation resistance in the light of global reprogramming remain poorly understood. Here we show that the Y-linked gene Rbmy1a1 is highly methylated in mature sperm and resists DNA demethylation post-fertilization...
January 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/28068325/trim28-interacts-with-ezh2-and-swi-snf-to-activate-genes-that-promote-mammosphere-formation
#6
J Li, Y Xi, W Li, R L McCarthy, S A Stratton, W Zou, W Li, S Y Dent, A K Jain, M C Barton
Histone methyl transferase EZH2 (Enhancer of Zeste Homolog 2) is generally associated with H3K27 methylation and gene silencing, as a member of the polycomb repressor 2 (PRC2) complex. Immunoprecipitation and mass spectrometry of the EZH2-protein interactome in estrogen receptor positive, breast cancer-derived MCF7 cells revealed EZH2 interactions with subunits of chromatin remodeler SWI/SNF complex and TRIM28, which formed a complex with EZH2 distinct from PRC2. Unexpectedly, transcriptome profiling showed that EZH2 primarily activates, rather than represses, transcription in MCF7 cells and with TRIM28 co-regulates a set of genes associated with stem cell maintenance and poor survival of breast cancer patients...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28052240/trim28-controls-a-gene-regulatory-network-based-on-endogenous-retroviruses-in-human-neural-progenitor-cells
#7
Per Ludvik Brattås, Marie E Jönsson, Liana Fasching, Jenny Nelander Wahlestedt, Mansoureh Shahsavani, Ronny Falk, Anna Falk, Patric Jern, Malin Parmar, Johan Jakobsson
Endogenous retroviruses (ERVs), which make up 8% of the human genome, have been proposed to participate in the control of gene regulatory networks. In this study, we find a region- and developmental stage-specific expression pattern of ERVs in the developing human brain, which is linked to a transcriptional network based on ERVs. We demonstrate that almost 10,000, primarily primate-specific, ERVs act as docking platforms for the co-repressor protein TRIM28 in human neural progenitor cells, which results in the establishment of local heterochromatin...
January 3, 2017: Cell Reports
https://www.readbyqxmd.com/read/27976729/interactomic-analysis-of-rest-nrsf-and-implications-of-its-functional-links-with-the-transcription-suppressor-trim28-during-neuronal-differentiation
#8
Namgyu Lee, Sung Jin Park, Ghazal Haddad, Dae-Kyum Kim, Seon-Min Park, Sang Ki Park, Kwan Yong Choi
RE-1 silencing transcription factor (REST) is a transcriptional repressor that regulates gene expression by binding to repressor element 1. However, despite its critical function in physiology, little is known about its interaction proteins. Here we identified 204 REST-interacting proteins using affinity purification and mass spectrometry. The interactome included proteins associated with mRNA processing/splicing, chromatin organization, and transcription. The interactions of these REST-interacting proteins, which included TRIM28, were confirmed by co-immunoprecipitation and immunocytochemistry, respectively...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905892/mir-491-regulates-glioma-cells-proliferation-by-targeting-trim28-in-vitro
#9
Zengxin Qi, Shengyong Cai, Jiajun Cai, Lingchao Chen, Yu Yao, Liang Chen, Ying Mao
BACKGROUND: MicroRNAs are significantly involved in tumorigenesis and progression of glioma. However, the critical part they play in glioma have not been fully elaborated. miR-491 and Tripartite motif containing 28 (TRIM28) are reported to aberrantly express in glioblastoma multiforme (GBM). Here, we detected miR-491 and TRIM28 expression and function in glioma cells. METHODS: We analyzed miR-491 expressions in 20 primary human GBM tissues and 6 control brain tissues by qRT-PCR assays and searched for The Cancer Genome Atlas (TCGA) database...
December 1, 2016: BMC Neurology
https://www.readbyqxmd.com/read/27845900/trim28-multi-domain-protein-regulates-cancer-stem-cell-population-in-breast-tumor-development
#10
Patrycja Czerwińska, Parantu K Shah, Katarzyna Tomczak, Marta Klimczak, Sylwia Mazurek, Barbara Sozańska, Przemysław Biecek, Konstanty Korski, Violetta Filas, Andrzej Mackiewicz, Jannik N Andersen, Maciej Wiznerowicz
The expression of Tripartite motif-containing protein 28 (TRIM28)/Krüppel-associated box (KRAB)-associated protein 1 (KAP1), is elevated in at least 14 tumor types, including solid and hematopoietic tumors. High level of TRIM28 is associated with triple-negative subtype of breast cancer (TNBC), which shows higher aggressiveness and lower survival rates. Interestingly, TRIM28 is essential for maintaining the pluripotent phenotype in embryonic stem cells. Following on that finding, we evaluated the role of TRIM28 protein in the regulation of breast cancer stem cells (CSC) populations and tumorigenesis in vitro and in vivo...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/27795446/transcriptional-silencing-of-moloney-murine-leukemia-virus-in-human-embryonic-carcinoma-cells
#11
Gary Z Wang, Stephen P Goff
: Embryonic carcinoma (EC) cells are malignant counterparts of embryonic stem (ES) cells and serve as useful models for investigating cellular differentiation and human embryogenesis. Though the susceptibility of murine EC cells to retroviral infection has been extensively analyzed, few studies of retrovirus infection of human EC cells have been performed. We tested the susceptibility of human EC cells to transduction by retroviral vectors derived from three different retroviral genera...
January 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27779468/trim28-regulates-the-nuclear-accumulation-and-toxicity-of-both-alpha-synuclein-and-tau
#12
Maxime Wc Rousseaux, Maria de Haro, Cristian A Lasagna-Reeves, Antonia De Maio, Jeehye Park, Paymaan Jafar-Nejad, Ismael Al-Ramahi, Ajay Sharma, Lauren See, Nan Lu, Luis Vilanova-Velez, Tiemo J Klisch, Thomas F Westbrook, Juan C Troncoso, Juan Botas, Huda Y Zoghbi
Several neurodegenerative diseases are driven by the toxic gain-of-function of specific proteins within the brain. Elevated levels of alpha-synuclein (α-Syn) appear to drive neurotoxicity in Parkinson's disease (PD); neuronal accumulation of tau is a hallmark of Alzheimer's disease (AD); and their increased levels cause neurodegeneration in humans and model organisms. Despite the clinical differences between AD and PD, several lines of evidence suggest that α-Syn and tau overlap pathologically. The connections between α-Syn and tau led us to ask whether these proteins might be regulated through a shared pathway...
October 25, 2016: ELife
https://www.readbyqxmd.com/read/27601472/o-glcnacase-is-an-rna-polymerase-ii-elongation-factor-coupled-to-pausing-factors-spt5-and-tif1%C3%AE
#13
Melissa Resto, Bong-Hyun Kim, Alfonso G Fernandez, Brian J Abraham, Keji Zhao, Brian A Lewis
We describe here the identification and functional characterization of the enzyme O-GlcNAcase (OGA) as an RNA polymerase II elongation factor. Using in vitro transcription elongation assays, we show that OGA activity is required for elongation in a crude nuclear extract system, whereas in a purified system devoid of OGA the addition of rOGA inhibited elongation. Furthermore, OGA is physically associated with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1β, and a purified OGA-SPT5-TIF1β complex has elongation properties...
October 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27432546/rna-polymerase-ii-promoter-proximal-pausing-in-mammalian-long-non-coding-genes
#14
Heeyoun Bunch, Brian P Lawney, Adam Burkholder, Duanduan Ma, Xiaofeng Zheng, Shmulik Motola, David C Fargo, Stuart S Levine, Yaoyu E Wang, Guang Hu
Mammalian genomes encode a large number of non-coding RNAs (ncRNAs) that greatly exceed mRNA genes. While the physiological and pathological roles of ncRNAs have been increasingly understood, the mechanisms of regulation of ncRNA expression are less clear. Here, our genomic study has shown that a significant number of long non-coding RNAs (lncRNAs, >1000 nucleotides) harbor RNA polymerase II (Pol II) engaged with the transcriptional start site. A pausing and transcriptional elongation factor for protein-coding genes, tripartite motif-containing 28 (TRIM28) regulates the transcription of a subset of lncRNAs in mammalian cells...
August 2016: Genomics
https://www.readbyqxmd.com/read/27420979/molecular-cloning-and-expression-vector-construction-of-bovine-trim28
#15
X Ma, Z C Zhai, M L Zhang, B H Song, Y R Zhu, S B Yang, X Q Dong, L Y Su, C F Wang, H X Ma, W M Luan
The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group...
June 24, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27412325/tripartite-motif-containing-28-trim28-promotes-breast-cancer-metastasis-by-stabilizing-twist1-protein
#16
Chunli Wei, Jingliang Cheng, Boxv Zhou, Li Zhu, Md Asaduzzaman Khan, Tao He, Sufang Zhou, Jian He, Xiaoling Lu, Hanchun Chen, Dianzheng Zhang, Yongxiang Zhao, Junjiang Fu
TRIM28 regulates its target genes at both transcriptional and posttranscriptional levels. Here we report that a TRIM28-TWIST1-EMT axis exists in breast cancer cells and TRIM28 promotes breast cancer metastasis by stabilizing TWIST1 and subsequently enhancing EMT. We find that TRIM28 is highly expressed in both cancer cell lines and advanced breast cancer tissues, and the levels of TRIM28 and TWIST1 are positively correlated with the aggressiveness of breast carcinomas. Overexpression and depletion of TRIM28 up- and down-regulates the protein, but not the mRNA levels of TWIST1, respectively, suggesting that TRIM28 upregulates TWIST1 post-transcriptionally...
July 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27364555/metabolic-stress-induced-phosphorylation-of-kap1-ser473-blocks-mitochondrial-fusion-in-breast-cancer-cells
#17
Chun-Ting Cheng, Ching-Ying Kuo, Ching Ouyang, Chien-Feng Li, Yiyin Chung, David C Chan, Hsing-Jien Kung, David K Ann
Mitochondrial dynamics during nutrient starvation of cancer cells likely exert profound effects on their capability for metastatic progression. Here, we report that KAP1 (TRIM28), a transcriptional coadaptor protein implicated in metastatic progression in breast cancer, is a pivotal regulator of mitochondrial fusion in glucose-starved cancer cells. Diverse metabolic stresses induced Ser473 phosphorylation of KAP1 (pS473-KAP1) in a ROS- and p38-dependent manner. Results from live-cell imaging and molecular studies revealed that during the first 6 to 8 hours of glucose starvation, mitochondria initially underwent extensive fusion, but then subsequently fragmented in a pS473-KAP1-dependent manner...
September 1, 2016: Cancer Research
https://www.readbyqxmd.com/read/27350605/trim28-is-an-epigenetic-barrier-to-induced-pluripotent-stem-cell-reprogramming
#18
Denise Catherine Miles, Nienke Alexandra de Vries, Santiago Gisler, Cor Lieftink, Waseem Akhtar, Ewa Gogola, Inka Pawlitzky, Danielle Hulsman, Ellen Tanger, Martijn Koppens, Roderick Leonardus Beijersbergen, Maarten van Lohuizen
Since the discovery of induced pluripotent stem cells there has been intense interest in understanding the mechanisms that allow a somatic cell to be reprogrammed back to a pluripotent state. Several groups have studied the alterations in gene expression that occur as somatic cells modify their genome to that of an embryonic stem cell. Underpinning many of the gene expression changes are modifications to the epigenetic profile of the associated chromatin. We have used a large-scale shRNA screen to identify epigenetic modifiers that act as barriers to reprogramming...
June 28, 2016: Stem Cells
https://www.readbyqxmd.com/read/27128603/7skiing-on-chromatin-move-globally-act-locally
#19
Iván D'Orso
RNA polymerase II (Pol II) pausing at promoter-proximal regions is a highly regulated step in the transcription cycle. Pause release is facilitated by the P-TEFb kinase, which phosphorylates Pol II and negative elongation factors. Recent studies suggest that P-TEFb (as part of the inhibitory 7SK snRNP) is recruited to promoter-proximal regions through interaction with KAP1/TRIM28/TIF1β to facilitate 'on-site' kinase activation and transcription elongation. Here, I discuss features of this model and future challenges to further hone our understanding of transcriptional regulation including Pol II pausing and pause release...
June 2, 2016: RNA Biology
https://www.readbyqxmd.com/read/27029610/atrx-binds-to-atypical-chromatin-domains-at-the-3-exons-of-zinc-finger-genes-to-preserve-h3k9me3-enrichment
#20
David Valle-García, Zulekha A Qadeer, Domhnall S McHugh, Flávia G Ghiraldini, Asif H Chowdhury, Dan Hasson, Michael A Dyer, Félix Recillas-Targa, Emily Bernstein
ATRX is a SWI/SNF chromatin remodeler proposed to govern genomic stability through the regulation of repetitive sequences, such as rDNA, retrotransposons, and pericentromeric and telomeric repeats. However, few direct ATRX target genes have been identified and high-throughput genomic approaches are currently lacking for ATRX. Here we present a comprehensive ChIP-sequencing study of ATRX in multiple human cell lines, in which we identify the 3' exons of zinc finger genes (ZNFs) as a new class of ATRX targets...
June 2, 2016: Epigenetics: Official Journal of the DNA Methylation Society
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