keyword
MENU ▼
Read by QxMD icon Read
search

immunoassay

keyword
https://www.readbyqxmd.com/read/29454301/heteroassembled-gold-nanoparticles-with-sandwich-immunoassay-lspr-chip-format-for-rapid-and-sensitive-detection-of-hepatitis-b-virus-surface-antigen-hbsag
#1
Jinwoon Kim, Seo Yeong Oh, Shruti Shukla, Seok Bok Hong, Nam Su Heo, Vivek K Bajpai, Hyang Sook Chun, Cheon-Ho Jo, Bong Gill Choi, Yun Suk Huh, Young-Kyu Han
This study aimed to develop a more sensitive method for the detection of hepatitis B surface antigen (HBsAg) using heteroassembled gold nanoparticles (AuNPs). A single layered localized surface plasmon resonance (LSPR) chip format was developed with antigen-antibody reaction-based detection symmetry using AuNPs, which detected HBsAg at 10 pg/mL. To further improve the detection limit, a modified detection format was fabricated by fixing a secondary antibody (to form a heteroassembled sandwich format) to the AuNP monolayer, which enhanced the detection sensitivity by about 100 times...
February 7, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29453818/the-association-between-thyroid-function-and-incidence-of-metabolic-syndrome-in-euthyroid-subjects-tianjin-chronic-low-grade-systemic-inflammation-and-health-cohort-study
#2
Yeqing Gu, Yanyan Wang, Qing Zhang, Li Liu, Ge Meng, Zhanxin Yao, Hongmei Wu, Yang Xia, Xue Bao, Hongbin Shi, Honglei Wang, Shaomei Sun, Xing Wang, Ming Zhou, Qiyu Jia, Kun Song, Kaijun Niu
OBJECTIVE: Thyroid hormones (THs) are primarily responsible for the regulation of energy homeostasis and metabolism. However, few prospective studies have assessed the association between THs and metabolic syndrome (MetS) in a general population. We therefore designed a cohort study to examine whether serum THs levels within the reference range are predictive factors for developing MetS in adults. DESIGN: Prospective cohort study. PARTICIPANTS: A prospective study (n=6,119) was performed in Tianjin, China...
February 17, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29453377/optimized-peptide-based-inhibitors-targeting-the-dihydrofolate-reductase-pathway-in-cancer
#3
Amrinder Singh, Neha Deshpande, Nilkamal Pramanik, Siddharth Jhunjhunwala, Annapoorni Rangarajan, Hanudatta S Atreya
We report the first peptide based hDHFR inhibitors designed on the basis of structural analysis of dihydrofolate reductase (DHFR). A set of peptides were rationally designed and synthesized using solid phase peptide synthesis and characterized using nuclear magnetic resonance and enzyme immunoassays. The best candidate among them, a tetrapeptide, was chosen based on molecular mechanics calculations and evaluated in human lung adenocarcinoma cell line A549. It showed a significant reduction of cell proliferation and an IC 50 of 82 µM was obtained...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453202/management-of-endocrine-disease-hyperandrogenic-states-in-women-pitfalls-in-laboratory-diagnosis
#4
Michel Pugeat, Ingrid Plotton, Aude Brac de la Perrière, Gerald Raverot, Henri Déchaud, Véronique Raverot
Measuring total testosterone level is the first-line approach in assessing androgen excess in women. The main pitfalls in measuring testosterone relate to its low concentration and to the structural similarity between circulating androgens and testosterone, requiring accurate techniques with high specificity and sensitivity. These goals can be achieved by immunoassay using a specific anti-testosterone monoclonal antibody, ideally after an extraction step. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) will be commonly used for measuring testosterone, providing optimal accuracy with a low limit of detection...
February 16, 2018: European Journal of Endocrinology
https://www.readbyqxmd.com/read/29452970/effect-of-tear-supplements-on-signs-symptoms-and-inflammatory-markers-in-dry-eye
#5
Eilidh Martin, Katherine M Oliver, E Ian Pearce, Alan Tomlinson, Peter Simmons, Suzanne Hagan
PURPOSE: Three tear supplements were compared for their effects on the signs, symptoms and inflammatory status of subjects with dry eye disease. Assessments were made before and after both 2 and 4 weeks of treatment. METHODS: In this masked, randomized, 3-way crossover trial, eighteen dry eye subjects were recruited. At each visit, symptoms, tear evaporation rate, stability and osmolarity were measured and tear samples were analyzed for 7 inflammatory markers, using multiplex immunoassays...
February 13, 2018: Cytokine
https://www.readbyqxmd.com/read/29452596/relationship-between-polyunsaturated-fatty-acid-composition-in-serum-phospholipids-systemic-low-grade-inflammation-and-glycemic-control-in-patients-with-type-2-diabetes-and-atherosclerotic-cardiovascular-disease
#6
Malgorzata Poreba, Pawel Rostoff, Aleksander Siniarski, Magdalena Mostowik, Renata Golebiowska-Wiatrak, Jadwiga Nessler, Anetta Undas, Grzegorz Gajos
BACKGROUND: There are inconsistent data about the role of serum phospholipid fatty acid composition in patients with type 2 diabetes (T2DM) and atherosclerotic cardiovascular disease (ASCVD). The aim of the study was to investigate the relationship between serum phospholipid fatty acid composition, systemic low-grade inflammation, and glycemic control in high-risk T2DM patients. METHODS: Seventy-four patients (26% women, mean age 65.6 ± 6.8 years) with T2DM (median diabetes duration 10 years) and documented ASCVD (74 with coronary artery disease, 26 with peripheral arterial disease) were enrolled in the study...
February 16, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29452421/the-short-cosyntropintest-revisited-new-normal-reference-range-using-lcmsms
#7
Grethe Å Ueland, Paal Methlie, Marianne Øksnes, Hrafnkell B Thordarson, Jørn Sagen, Ralf Kellmann, Gunnar Mellgren, Maria Ræder, Per Dahlqvist, Sandra R Dahl, Per M Thorsby, Kristian Løvås, Eystein S Husebye
Background: The cosyntropin test is used to diagnose adrenal insufficiency (AI) and non-classical congenital adrenal hyperplasia (NCCAH). Current cutoffs for cortisol and 17-hydroxyprogesterone (17-OHP) are derived from non-standardized immunoassays. Liquid chromatography tandem mass spectrometry (LCMSMS) offers direct measurement of steroids, prompting the need to re-establish normal ranges. Aim: Define cutoff values for cortisol and 17-OHP in serum by LCMSMS 30 and 60 minutes after intravenous administration of 250 µg tetracosactide acetate (Synachten©) to healthy volunteers, and compare LCMSMS with routine immunoassays...
February 14, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29451618/the-association-of-vitamin-d-levels-and-the-frailty-phenotype-among-non-geriatric-dialysis-patients-a-cross-sectional-study
#8
Demet Tekdos Demircioglu
OBJECTIVES: The aim of this study was to investigate the frequency of frailty and the association of vitamin D levels and the frailty phenotype among non-geriatric dialysis patients. METHOD: Seventy-four stable, chronic hemodialysis patients from the hemodialysis unit of the hospital were enrolled in the study. The patients' medical histories and laboratory findings were obtained from the medical records of the dialysis unit. Serum parathyroid hormone and 25-hydroxy vitamin D levels were determined using chemiluminometric immunoassays...
2018: Clinics
https://www.readbyqxmd.com/read/29451408/single-nucleotide-polymorphisms-in-key-one-carbon-metabolism-genes-and-their-association-with-blood-folate-and-homocysteine-levels-in-a-chinese-population-in-yunnan
#9
Juan Ni, Yaoxian Liu, Tao Zhou, Xiayu Wu, Xu Wang
OBJECTIVE: One-carbon metabolism (OCM) is essential for DNA synthesis and methylation. Single nucleotide polymorphisms (SNPs) within OCM genes may affect folic acid (FA) metabolism, disrupt homocysteine (Hcy) homeostasis, and increase the risk of disease. This study investigated the relationship between SNPs in key OCM genes and their association with blood FA and Hcy levels in a healthy population in Yunnan, China. METHODS: Six SNPs within five key OCM genes (MTHFR C677T, MTHFR A1298C, MS A2756G, MTRR A66G, CBS T833C, and SHMT C1420T) were genotyped in 300 healthy volunteers (148 males and 152 females) using polymerase chain reaction/restriction fragment length polymorphism...
February 16, 2018: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/29450585/effect-of-isolated-vitamin-d-supplementation-on-bone-turnover-markers-in-younger-postmenopausal-women-a-randomized-double-blind-placebo-controlled-trial
#10
J Nahas-Neto, L M Cangussu, C L Orsatti, F N Bueloni-Dias, P F Poloni, E B Schmitt, E A P Nahas
Vitamin D (VD) plays an important role in bone mineralization. The present study investigates the effect of VD supplementation alone on bone turnover markers in younger postmenopausal women. It has been shown that VD supplementation in postmenopausal women with hypovitaminosis D is associated with a reduction in bone turnover markers. PURPOSE: The purpose of this study is to evaluate the effect of VD supplementation alone on bone turnover markers in younger postmenopausal women. METHODS: In this double-blind, placebo-controlled trial, 160 women were randomized into the VD group (supplementation with 1000 IU of vitamin D3/day, orally; n = 80) or placebo group (n = 80)...
February 15, 2018: Osteoporosis International
https://www.readbyqxmd.com/read/29449777/a-homogeneous-time-resolved-fluorescence-immunoassay-method-for-the-measurement-of-compound-w
#11
Biao Huang, Huixin Yu, Jiandong Bao, Manda Zhang, William L Green, Sing-Yung Wu
Objective: Using compound W (a 3,3'-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29449597/substructure-activity-relationship-studies-on-antibody-recognition-for-phenylurea-compounds-using-competitive-immunoassay-and-computational-chemistry
#12
Fuyuan Zhang, Bing Liu, Guozhen Liu, Yan Zhang, Junping Wang, Shuo Wang
Based on the structural features of fluometuron, an immunizing hapten was synthesized and conjugated to bovine serum albumin as an immunogen to prepare a polyclonal antibody. However, the resultant antibody indicated cross-reactivity with 6 structurally similar phenylurea herbicides, with binding activities (expressed by IC 50 values) ranging from 1.67 µg/L to 42.71 µg/L. All 6 phenylurea herbicides contain a common moiety and three different substitutes. To understand how these three different chemical groups affect the antibody-phenylurea recognition activity, quantum chemistry, using density function theory (DFT) at the B3LYP/6-311++ G(d,p) level of theory, was employed to optimize all phenylurea structures, followed by determination of the 3D conformations of these molecules, pharmacophore analysis, and molecular electrostatic potential (ESP) analysis...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29449526/raised-serum-levels-of-syndecan-1-cd138-in-a-case-of-acute-idiopathic-systemic-capillary-leak-syndrome-scls-clarkson-s-disease
#13
Ole Wilhelm Bøe, Kjell Sveen, Magne Børset, Kirk M Druey
BACKGROUND Systemic capillary leak syndrome (SCLS) (Clarkson's disease) is a rare disorder of unknown etiology, characterized by transient episodes of hypotension, and the microvascular leak of fluids into the peripheral tissues, resulting in edema. Between 80-90% of patients with SCLS have a concomitant monoclonal gammopathy. Although translational in vitro studies have implicated vascular endothelial barrier dysfunction in the etiology of SCLS, the etiology and disease associations in clinical cases remain unknown...
February 16, 2018: American Journal of Case Reports
https://www.readbyqxmd.com/read/29448519/peroxidase-like-activity-of-gold-nanoparticles-and-their-gold-staining-enhanced-elisa-application
#14
Doudou Lou, Yanyan Tian, Yu Zhang, Junjie Yin, Ting Yang, Chuan He, Ming Ma, Wei Yu, Ning Gu
We found that the peroxidase-like activity of gold nanoparticles (GNPs) followed the Michaelis-Menten kinetic model and was dependent on environmental pH and temperature, which was very similar to natural Horseradish Peroxidase (HRP). However, unlike HRP, which needs a lower H2O2 concentration with a very narrow range to reach a maximum reaction rate and avoid enzyme poisoning, GNPs have very high activity, even at an H2O2 concentration two orders of magnitude higher than HRP. It was demonstrated that H2O2 treatment could enhance the peroxidase-like activity of GNPs, resulting thus in the activity increase in a circular catalytic reaction by the reduplicative use of GNPs...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448045/serum-architect-pivka-ii-reference-interval-in-healthy-chinese-adults-sub-analysis-from-a-prospective-multicenter-study
#15
Cunling Yan, Jian Hu, Jia Yang, Zhaoyun Chen, Huijun Li, Lianhua Wei, Wei Zhang, Hao Xing, Guoyao Sang, Xiaoqin Wang, Ruilin Han, Ping Liu, Zhihui Li, Zhiyan Li, Ying Huang, Li Jiang, Shunjun Li, Shuyang Dai, Nianyue Wang, Yongfeng Yang, Li Ma, Andrew Soh, Agim Beshiri, Feng Shen, Tian Yang, Zhuping Fan, Yijie Zheng, Wei Chen
BACKGROUND: Protein induced by vitamin K absence or antagonist-II (PIVKA-II) has been widely used as a biomarker for liver cancer diagnosis in Japan for decades. However, the reference intervals for serum ARCHITECT PIVKA-II have not been established in the Chinese population. Thus, this study aimed to measure serum PIVKA-II levels in healthy Chinese subjects. METHODS: This is a sub-analysis from the prospective, cross-sectional and multicenter study (ClinicalTrials...
February 12, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29447912/immunogold-agglutination-assay-for-direct-detection-of-hpv-16-e6-and-l1-proteins-from-clinical-specimens
#16
Parvapan Bhattarakosol, Kamolwan Plaignam, Amornpun Sereemaspun
HPV-16 infection is the most common cause of cervical cancer. As HPV-16 transforms the cell, E6 oncoprotein is over-expressed. Therefore, molecular detection of HPV-16 E6 mRNA is now being used for diagnosis and prediction of cancer development. Besides detecting E6 mRNA, a rapid lateral flow detecting the E6 protein using enzyme immunoassay is also now on market with a sensitivity of 53.5% for cervical intraepithelial neoplasia (CIN)-3 or more severe (CIN-3 + ). Here, an immunogold-agglutination assay was developed to detect not only HPV-16 E6 protein but also L1, a major capsid protein found in the productive stage of the virus...
February 12, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29447367/endometrial-natural-killer-nk-cells-reveal-a-tissue-specific-receptor-repertoire
#17
D Feyaerts, T Kuret, B van Cranenbroek, S van der Zeeuw-Hingrez, O W H van der Heijden, A van der Meer, I Joosten, R G van der Molen
STUDY QUESTION: Is the natural killer (NK) cell receptor repertoire of endometrial NK (eNK) cells tissue-specific? SUMMARY ANSWER: The NK cell receptor (NKR) expression profile in pre-pregnancy endometrium appears to have a unique tissue-specific phenotype, different from that found in NK cells in peripheral blood, suggesting that these cells are finely tuned towards the reception of an allogeneic fetus. WHAT IS KNOWN ALREADY: NK cells are important for successful pregnancy...
February 13, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29446920/homogeneous-noncompetitive-luminescent-immunodetection-of-small-molecules-by-ternary-protein-fragment-complementation
#18
Yuki Ohmuro-Matsuyama, Hiroshi Ueda
The homogeneous immunological detection of small molecules at high sensitivity is still a daunting task. Here, we tried sensitive noncompetitive detection of small peptides based on the open-sandwich immunoassay principle, which was combined with a bioluminescent protein-fragment complementation assay (PCA) in vitro. Since the detection of antigen-induced approximation of the two antibody variable region fragments V H and V L by the standard Nanoluc-based PCA utilizing larger (LgBiT) and shorter (SmBiT) fragments was not successful, we decided to further split LgBiT into two, yielding smaller N-terminal derivative (LnBiT) and two C-terminal, 11 residue peptides (LcBiT and SmBiT) corresponding to consecutive beta strands, to which V H and V L were each fused and expressed in Escherichia coli cells...
February 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29446612/total-capture-convection-limited-nanofluidic-immunoassays-exhibiting-nanoconfinement-effects
#19
Casey J Galvin, Kentaro Shirai, Ali Rahmani, Kakuta Masaya, Amy Q Shen
Understanding nanoconfinement phenomena is necessary to develop nanofluidic technology platforms. One example of nanoconfinement phenomena are shifts in reaction equilibria toward reaction products in nanoconfined systems, which have been predicted theoretically and observed experimentally in DNA hybridization. Here we demonstrate a convection limited nanofluidic immunoassay that achieves total capture of a target analyte, and an apparent shift in the antibody-antigen reaction equilibrium due to nanoconfinement...
February 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29446525/laboratory-testing-for-factor-viii-and-ix-inhibitors-in-haemophilia-a-review
#20
REVIEW
C H Miller
Inhibitors are antibodies directed against haemophilia treatment products which interfere with their function. Factor VIII (FVIII) inhibitors in haemophilia A and factor IX (FIX) inhibitors in haemophilia B are significant clinically when they require a change in a patient's treatment regimen. Their persistence may increase morbidity and mortality. Multiple laboratory tests are now available for detecting and understanding inhibitors in haemophilia. Inhibitors are traditionally measured by their interference in clotting or chromogenic factor assays...
February 15, 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
keyword
keyword
63886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"