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https://www.readbyqxmd.com/read/29218459/discordance-between-mass-spectrometry-and-immunometric-igf-1-assay-in-pituitary-disease-a-prospective-study
#1
Vivien Bonert, John Carmichael, Zengru Wu, James Mirocha, Daniel A Perez, Nigel J Clarke, Richard E Reitz, Michael J McPhaul, Adam Mamelak
PURPOSE: Measuring IGF-1, a biomarker for GH activity, is critical to evaluating disordered hypothalamic-pituitary GH axis. Inconsistent IGF-1 measurements among different immunoassays are well documented. We switched from Immulite 2000 immunoassay to narrow-mass-extraction, high-resolution liquid chromatography mass-spectrometry (LC-MS) compliant with recent consensus recommendations on assay standardization. Comparability of these two assays in patients with pituitary disease in a clinical practice setting is not known...
December 7, 2017: Pituitary
https://www.readbyqxmd.com/read/29217402/inflammatory-profile-analysis-reveals-differences-in-cytokine-expression-between-smokers-moist-snuff-users-and-dual-users-compared-to-non-tobacco-consumers
#2
Judi Azevedo Sgambato, Bobbette A Jones, John W Caraway, G L Prasad
OBJECTIVE AND DESIGN: The aim of this study is to investigate the inflammatory alterations due to the use of smokeless tobacco and dual use of smokeless tobacco and cigarettes, relative to smoking. SUBJECTS: Plasma and saliva samples were collected from healthy smokers (SMK-100 subjects), moist snuff users (MSC-89 subjects), the dual users (DUSMK-49 subjects), and non-tobacco consumers (NTC-99 subjects) from two cross-sectional studies. METHODS: Luminex Human InflammationMAP® 1...
December 4, 2017: Cytokine
https://www.readbyqxmd.com/read/29215864/platinum-nanocatalyst-amplification-redefining-the-gold-standard-for-lateral-flow-immunoassays-with-ultra-broad-dynamic-range
#3
Colleen N Loynachan, Michael R Thomas, Eleanor R Gray, Daniel A Richards, Jeongyun Kim, Benjamin S Miller, Jennifer Clare Brookes, Shweta Agarwal, Vijay Chudasama, Rachel A McKendry, Molly M Stevens
Paper-based lateral flow immunoassays (LFIAs) are one of the most widely used point-of-care (PoC) devices, however, their application in early disease diagnostics is often limited due to insufficient sensitivity for the requisite sample sizes and the short timeframes of PoC testing. To address this, we developed a serum-stable, nanoparticle catalyst-labelled LFIA with a sensitivity surpassing that of both current commercial and published sensitivities for paper-based detection of p24, one of the earliest and most conserved biomarkers of HIV...
December 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/29215565/a-miniaturized-impedimetric-immunosensor-for-the-competitive-detection-of-adrenocorticotropic-hormone
#4
Nan Li, Egor M Larin, Kagan Kerman
Adrenocorticotropic hormone (ACTH) plays an essential role in regulating corticosteroid hormone production, which has important functions in a myriad of critical physiological functions. In this proof-of-concept study, a miniaturized immunosensor was developed for the highly sensitive detection of ACTH using electrochemical impedance spectroscopy (EIS) in connection with disposable screen-printed gold electrodes (SPGEs). A film of 3,3'-dithiobis[sulfosuccinimidylpropionate] (DTSSP) was prepared to immobilize anti-ACTH antibodies covalently on the nanostructured SPGE surface...
December 7, 2017: Sensors
https://www.readbyqxmd.com/read/29215518/angiogenic-factor-profiles-in-pregnant-women-with-a-history-of-early-onset-severe-preeclampsia-receiving-low-molecular-weight-heparin-prophylaxis
#5
Edouard Lecarpentier, Jean Christophe Gris, Eva Cochery-Nouvellon, Erick Mercier, Cyril Touboul, Ravi Thadhani, S Ananth Karumanchi, Bassam Haddad
OBJECTIVE: To evaluate whether daily low-molecular-weight (LMW) heparin prophylaxis during pregnancy alters profile of circulating angiogenic factors that have been linked with the pathogenesis of preeclampsia and fetal growth restriction. METHODS: This is a planned ancillary study of the Heparin-Preeclampsia trial, a randomized trial in pregnant women with a history of severe early-onset preeclampsia (less than 34 weeks of gestation). In the parent study, all women were treated with aspirin and then randomized to receive LMW heparin or aspirin alone...
December 4, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29215325/dosikit-a-new-portable-immunoassay-for-fast-external-irradiation-biodosimetry
#6
Julie Bensimon Etzol, Marco Valente, Sandrine Altmeyer, Caroline Bettencourt, Samuel Bouvet, Guillaume Cosler, François Desangles, Michel Drouet, Fabrice Entine, Francis Hérodin, Flora Jourquin, Yannick Lecompte, Patrick Martigne, Xavier Michel, Jérôme Pateux, Nicolas Ugolin, Sylvie Chevillard
DosiKit is a new field-radiation biodosimetry immunoassay for rapid triage of individuals exposed to external total-body radiation. Here, we report on the validation of this immunoassay in human blood cell extracts 0.5 h after in vitro exposure to 137Cs gamma rays, using γ-H2AX analysis. First, calibration curves were established for five donors at doses ranging from 0 to 10 Gy and dose rates ranging from ∼0.8 to ∼3 Gy/min. The calibration curves, together with a γ-H2AX peptide scale, enabled the definition of inter-experimental correction factors...
December 7, 2017: Radiation Research
https://www.readbyqxmd.com/read/29214751/multi-center-performance-evaluations-of-tacrolimus-and-cyclosporine-electrochemiluminescence-immunoassays-in-the-asia-pacific-region
#7
Xuzhen Qin, Jianzhong Rui, Yong Xia, Hong Mu, Sang Hoon Song, Raja Elina Raja Aziddin, Gabrielle Miles, Yuli Sun, Sail Chun
BACKGROUND: The immunosuppressant drugs (ISDs), tacrolimus and cyclosporine, are vital for solid organ transplant patients to prevent rejection. However, toxicity is a concern, and absorption is highly variable across patients; therefore, ISD levels need to be precisely monitored. In the Asia-Pacific (APAC) region, tacrolimus and cyclosporine concentrations are typically measured using immunoassays. The objective of this study was to assess the analytical performance of Roche Elecsystacrolimus and cyclosporinee electrochemiluminescence immunoassays (ECLIAs)...
March 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29214218/skiving-stacked-sheets-of-paper-into-test-paper-for-rapid-and-multiplexed-assay
#8
Mingzhu Yang, Wei Zhang, Junchuan Yang, Binfeng Hu, Fengjing Cao, Wenshu Zheng, Yiping Chen, Xingyu Jiang
This paper shows that stacked sheets of paper preincubated with different biological reagents and skiving them into uniform test paper sheets allow mass manufacturing of multiplexed immunoassay devices and simultaneous detection of multiplex targets that can be read out by a barcode scanner. The thickness of one sheet of paper can form the width of a module for the barcode; when stacked, these sheets of paper can form a series of barcodes representing the targets, depending on the color contrast provided by a colored precipitate of an immunoassay...
December 2017: Science Advances
https://www.readbyqxmd.com/read/29214121/towards-one-step-quantitation-of-prostate-specific-antigen-psa-in-microfluidic-devices-feasibility-of-optical-detection-with-nanoparticle-labels
#9
Ana I Barbosa, Jan H Wichers, Aart van Amerongen, Nuno M Reis
Rapid and quantitative prostate-specific antigen (PSA) biomarker detection would be beneficial to cancer diagnostics, improving early detection and therefore increasing chances of survival. Nanoparticle-based detection is routinely used in one-step nitrocellulose-based lateral flow (LF) immunoassays; however, it is well established within the scientific diagnostic community that LF technology lacks sensitivity for measuring biomarkers, such as prostate-specific antigen (PSA). A trend in point-of-care (POC) protein biomarker quantitation is the miniaturization of immunoassays in microfluidic devices...
2017: BioNanoScience
https://www.readbyqxmd.com/read/29213336/adiponectin-resistin-interplay-in-serum-and-in-adipose-tissue-of-obese-and-normal-weight-individuals
#10
Marta Izabela Jonas, Alina Kurylowicz, Zbigniew Bartoszewicz, Wojciech Lisik, Maurycy Jonas, Justyna Domienik-Karlowicz, Monika Puzianowska-Kuznicka
Background: The interplay between adiponectin and resistin, the two adipokines of opposite effects, may determine the metabolic profile of obese individuals and development of obesity-related complications. The current study was conducted to assess how adiponectin/resistin interplay in sera and adipose tissues may influence the metabolic profile of obese and normal-weight subjects. Methods: Concentrations of adiponectin and resistin were measured on protein level by immunoassay in visceral and subcutaneous adipose tissues from 50 obese (body mass index > 40 kg/m2) and 28 normal-weight (body mass index 20-24...
2017: Diabetology & Metabolic Syndrome
https://www.readbyqxmd.com/read/29212942/factors-leading-to-the-loss-of-natural-elite-control-of-hiv-1-infection
#11
María Pernas, Laura Tarancón-Diez, Esther Rodríguez-Gallego, Josep Gómez, Julia G Prado, Concepción Casado, Beatriz Dominguez-Molina, Isabel Olivares, Maite Coiras, Agathe León, Carmen Rodriguez, Jose Miguel Benito, Norma Rallón, Montserrat Plana, Onofre Martinez-Madrid, Marta Dapena, Jose Antonio Iribarren, Jorge Del Romero, Felipe García, José Alcamí, M Ángeles Muñoz-Fernández, Francisco Vidal, Manuel Leal, Cecilio Lopez-Galindez, Ezequiel Ruiz-Mateos
HIV-1 elite controllers (EC) maintain undetectable viral load (VL) in the absence of antiretroviral treatment. However, these subjects have heterogeneous clinical outcomes including a proportion loosing HIV-1 control over time. In this work we compared, in a longitudinal design, transient EC, analyzed before and after the loss of virological control, versus persistent EC. The aim was to identify factors leading to the loss of natural virological control of HIV-1-infection with a longitudinal retrospective study design...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29212594/-false-positive-troponin-i-due-to-heterophile-antibodies
#12
Henrik Nørlund, Ann Bovin
The troponin I (TnI) level is essential in the diagnosis of acute myocardial infarction, but may be elevated by other cardial and non-cardial causes. We report a 32-year-old pregnant woman admitted to hospital because of chest pain and dyspnoea. TnI levels were significantly elevated, although there was no suspicion of acute coronary syndrome or myocarditis, and supplementary diagnostics excluded pulmonary embolism. The TnI level was found to be falsely elevated due to immunoassay interference. We stress the need to be aware of the possibility of false positive TnI, especially during pregnancy...
December 4, 2017: Ugeskrift for Laeger
https://www.readbyqxmd.com/read/29212463/could-a-remarkable-decrease-in-leptin-and-insulin-levels-from-colostrum-to-mature-milk-contribute-to-early-growth-catch-up-of-sga-infants
#13
Marina Nunes, Clécio Homrich da Silva, Vera Lucia Bosa, Juliana Rombaldi Bernardi, Isabel Cristina Ribas Werlang, Marcelo Zubaran Goldani
BACKGROUND: Breast milk is known to contain many bioactive hormones and peptides, which can influence infant growth and development. In this context, the purpose of this study was to evaluate the influence of different clinical pregnancy conditions on hormone concentrations in colostrum and mature breast milk. METHODS: An observational study was performed with mother-newborn pairs divided into five groups according to maternal clinical background: diabetes (12), hypertension (5), smoking (19), intrauterine growth restriction of unknown causes with small-for-gestational-age newborns at delivery (12), and controls (21)...
December 6, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29211962/highly-sensitive-and-rapid-detection-of-salmonella-typhimurium-using-a-colorimetric-paper-based-analytical-device-coupled-with-immunomagnetic-separation
#14
Monpichar Srisa-Art, Katherine E Boehle, Brian J Geiss, Charles S Henry
Salmonella causes over a million foodborne illnesses per year in the United States resulting in more hospitalizations and deaths than any other foodborne bacteria pathogen. To help prevent outbreaks a rapid, portable, sensitive, and reliable method for onsite detection of bacteria that can be used in different sample matrices would be beneficial. Herein, we present a colorimetric paper-based analytical device (PAD) combined with immunomagnetic separation (IMS) for detecting Salmonella typhimurium. IMS anti-Salmonella coated magnetic beads was applied to capture and separate bacteria from the sample matrix and preconcentrated it into small volumes before testing on paper...
December 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29211830/antibiotic-utilization-and-the-role-of-suspected-and-diagnosed-mosquito-borne-illness-among-adults-and-children-with-acute-febrile-illness-in-pune-india
#15
Matthew Robinson, Dileep Kadam, Anju Kagal, Sandhya Khadse, Aarti Kinikar, Chhaya Valvi, Anita Basavaraj, Renu Bharadwaj, Ivan Marbaniang, Savita Kanade, Priyanka Raichur, Jonathan Sachs, Eili Klein, Sara Cosgrove, Amita Gupta, Vidya Mave
Background: Antibiotic resistance mechanisms originating in low- and middle- income countries are among the most common worldwide. Reducing unnecessary antibiotic use in India, the world's largest antibiotic consumer is crucial to control antimicrobial resistance globally. Limited data describing factors influencing Indian clinicians to start or stop antibiotics is available. Methods: Febrile adults and children admitted to a public tertiary care hospital in Pune, India were enrolled...
December 4, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29210975/multi-center-evaluation-of-a-new-electrochemiluminescence-immunoassay-eclia-for-everolimus-concentrations-in-whole-blood
#16
Alain G Verstraete, Raül Rigo-Bonnin, Pierre Wallemacq, Michael Vogeser, Andre Schuetzenmeister, Christian Schmiedel, Maria Shipkova
BACKGROUND: The precise monitoring of everolimus, an immunosuppressant drug, is vital for transplant recipients due to its narrow therapeutic range. This study evaluated the analytical performance of a new electrochemiluminescence immunoassay (ECLIA) for everolimus concentrations in whole blood. METHODS: Accuracy, imprecision, and sensitivity studies for the Roche Elecsys everolimus ECLIA were performed at five European laboratories. The ECLIA was compared with liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods, as well as the Quantitative Microsphere System (QMS) everolimus assay...
November 28, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/29210527/seroprevalence-of-helicobacter-pylori-in-hispanics-living-in-puerto-rico-a-population-based-study
#17
María González-Pons, Marievelisse Soto-Salgado, Javier Sevilla, Juan M Márquez-Lespier, Douglas Morgan, Cynthia M Pérez, Marcia Cruz-Correa
BACKGROUND: Helicobacter pylori is an important etiologic factor for peptic ulcers and gastric cancer, one of the top ten leading causes of cancer death in Puerto Rico. However, the prevalence of H. pylori infections in this population was previously unknown. The aim of this study was to examine the seroprevalence of H. pylori and its associated risk factors in Puerto Rico. MATERIALS AND METHODS: A cross-sectional study was designed using an existing population-based biorepository...
December 6, 2017: Helicobacter
https://www.readbyqxmd.com/read/29210105/systematic-review-and-meta-analysis-of-diagnostic-accuracy-of-serum-refractometry-and-brix-refractometry-for-the-diagnosis-of-inadequate-transfer-of-passive-immunity-in-calves
#18
REVIEW
S Buczinski, E Gicquel, G Fecteau, Y Takwoingi, M Chigerwe, J M Vandeweerd
BACKGROUND: Transfer of passive immunity in calves can be assessed by direct measurement of immunoglobulin G (IgG) by methods such as radial immunodiffusion (RID) or turbidimetric immunoassay (TIA). IgG can also be measured indirectly by methods such as serum refractometry (REF) or Brix refractometry (BRIX). OBJECTIVES: To determine the accuracy of REF and BRIX for assessment of inadequate transfer of passive immunity (ITPI) in calves. DESIGN: Systematic review and meta-analysis of diagnostic accuracy studies...
December 5, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29209767/neuropathology-of-iatrogenic-creutzfeldt-jakob-disease-and-immunoassay-of-french-cadaver-sourced-growth-hormone-batches-suggest-possible-transmission-of-tauopathy-and-long-incubation-periods-for-the-transmission-of-abeta-pathology
#19
Charles Duyckaerts, Véronique Sazdovitch, Kunie Ando, Danielle Seilhean, Nicolas Privat, Zehra Yilmaz, Laurène Peckeu, Elodie Amar, Emmanuel Comoy, Aleksandra Maceski, Sylvain Lehmann, Jean-Pierre Brion, Jean-Philippe Brandel, Stéphane Haïk
Abeta deposits and tau pathology were investigated in 24 French patients that died from iatrogenic Creutzfeldt-Jakob disease after exposure to cadaver-derived human growth hormone (c-hGH) in the 1980s. Abeta deposits were found only in one case that had experienced one of the longest incubation periods. Three cases had also intracellular tau accumulation. The analysis of 24 batches of c-hGH, produced between 1974 and 1988, demonstrated for the first time the presence of Abeta and tau contaminants in c-hGH (in 17 and 6 batches, respectively)...
November 22, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/29209484/correlation-between-saliva-and-serum-concentrations-of-estradiol-in-women-undergoing-ovarian-hyperstimulation-with-gonadotropins-for-ivf-icsi
#20
C Dielen, T Fiers, S Somers, E Deschepper, J Gerris
Aim of the study: To examine saliva- and serum concentrations correlation of estradiol (E2) in women undergoing ovarian hyperstimulation for IVF/ICSI. Saliva measurements could simplify stimulation follow up. A 'home' test for E2 could be useful. Methods: Prospective interventional academic monocentric study at the Centre for Reproductive Medicine of the University Hospital of Ghent, Belgium. Between November 2014 and August 2015 thirty-one patients were included after random selection (inclusion criteria: < 41 years of age, any rank of IVF/ICSI cycle, serum anti- Müllerian hormone concentration ≥ 1 μg/L, treatment completely at the University Hospital...
June 2017: Facts, Views & Vision in ObGyn
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