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https://www.readbyqxmd.com/read/28719891/serum-interleukin-10-levels-correlate-with-cerebrospinal-fluid-amyloid-beta-deposition-in-alzheimer-disease-patients
#1
Lucio D'Anna, Samir Abu-Rumeileh, Martina Fabris, Cinzia Pistis, Antonio Baldi, Nova Sanvilli, Francesco Curcio, Gian Luigi Gigli, Sebastiano D'Anna, Mariarosaria Valente
BACKGROUND AND OBJECTIVE: In Alzheimer disease (AD) inflammation becomes evident throughout the course of the disease. However, the association between inflammation, cognitive impairment, and cerebrospinal biomarkers (Aβ42, t-tau, p-tau181, and Aβ42/p-tau181 ratio) is poorly understood. METHODS: A large panel of inflammatory cytokines (interleukin [IL]-1β, IL-1ra, IL-2, IL-4, IL-6, IL-10, IL-17, interferon-γ, tumor necrosis factor-α, and vascular endothelial growth factor) was analyzed using a multiplex immunoassay in 27 patients with a diagnosis of AD dementia and in 18 control subjects...
July 19, 2017: Neuro-degenerative Diseases
https://www.readbyqxmd.com/read/28719742/integrated-smartphone-app-chip-system-for-on-site-ppb-level-colorimetric-quantitation-of-aflatoxins
#2
Xiaochun Li, Fan Yang, Jessica X H Wong, Hua-Zhong Yu
We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app...
July 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28719278/evaluation-of-the-field-performance-of-immunocard-stat-%C3%A2-rapid-diagnostic-test-for-rotavirus-in-dadaab-refugee-camp-and-at-the-kenya-somalia-border
#3
Maurice Ope, Raymond Nyoka, Ahmed Unshur, Fredrick O Oyier, Shafe A Mowlid, Brian Owino, Steve B Ochieng, Charles I Okello, Joel M Montgomery, Burton Wagacha, Aleksandar Galev, Abdikadir Abdow, Mathew D Esona, Jacqueline Tate, David Fitter, Susan T Cookson, Balajee Arunmozhi, Nina Marano
AbstractRotavirus commonly causes diarrhea in children, leading to hospitalization and even death. Rapid diagnostic tests are feasible alternatives for determining rotavirus outbreaks in refugee camps that have inadequate laboratory capacity. We evaluated the field performance of ImmunoCard STAT!(®) Rotavirus (ICS-RV) in Dadaab Refugee Camp and at the Kenya-Somalia border. From May to December 2014, we prospectively enrolled children aged < 5 years hospitalized with acute diarrhea, defined as ≥ 3 episodes of loose stool in 24 hours for < 7 days...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28719014/bioavailability-and-tolerability-of-nebulised-dexamethasone-sodium-phosphate-in-adult-horses
#4
A D Haspel, S Giguère, K A Hart, L J Berghaus, J L Davis
BACKGROUND: Nebulisation of the injectable dexamethasone sodium phosphate (DSP) would offer an inexpensive way of delivering a potent corticosteroid directly to the lungs of horses with asthma. However, this approach would be advantageous only if systemic absorption is minimal and if the preservatives present in the formulation do not induce airway inflammation. OBJECTIVE: To investigate the bioavailability of nebulised DSP and to determine whether it induces airway inflammation or hypothalamic-pituitary-adrenal (HPA) axis suppression in healthy adult horses...
July 18, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28718488/infectious-disease-morbidity-and-growth-among-young-hiv-exposed-uninfected-children-in-jamaica
#5
Russell B Pierre, Toni-Anne Fulford, Kaye Lewis, Paulette Palmer, Christine Walters, Celia D C Christie
Objective: There is a growing body of data that demonstrates increased infectious disease outcomes for HIV-exposed uninfected (HIV-EU) infants as compared to their HIV-unexposed (HU) counterparts. We hypothesized that these HIV-EU infants are at greater risk for infectious morbidity and mortality when compared to the general childhood population. We therefore aimed to characterize infections and growth outcomes among HIV-EU infants in Jamaica during their first two years of life. By identifying these outcomes, specific interventions could be implemented to mitigate this risk of morbidity and mortality...
December 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28718270/salmonella-infection-amongst-food-workers-in-lahore
#6
Sajjad Ullah, Saira Bashir, Akif Qasim
This descriptive observational study was conducted at Pathology lab Jinnah hospital, Lahore, January 2013 to October 2015. We include all the food workers in different restaurants, slaughters, inn, recreation centre and bakeries of Lahore for the purpose to identify the salmonella infection, we used Typhoid IgGI/IgM Rapid test device methods (Serum/Plasma), is a lateral flow chromatographic immunoassay technique were calculated. We analysed 1025 individuals, 62 (6%) were found positive for the said diseases out of which 14 (1...
April 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28716979/blood-biomarkers-for-the-early-diagnosis-of-stroke-the-stroke-chip-study
#7
Alejandro Bustamante, Elena López-Cancio, Sara Pich, Anna Penalba, Dolors Giralt, Teresa García-Berrocoso, Carles Ferrer-Costa, Teresa Gasull, María Hernández-Pérez, Mónica Millan, Marta Rubiera, Pedro Cardona, Luis Cano, Helena Quesada, Mikel Terceño, Yolanda Silva, Mar Castellanos, Moisés Garces, Silvia Reverté, Xavier Ustrell, Rafael Marés, Joan Josep Baiges, Joaquín Serena, Francisco Rubio, Eduardo Salas, Antoni Dávalos, Joan Montaner
BACKGROUND AND PURPOSE: Stroke diagnosis could be challenging in the acute phase. We aimed to develop a blood-based diagnostic tool to differentiate between real strokes and stroke mimics and between ischemic and hemorrhagic strokes in the hyperacute phase. METHODS: The Stroke-Chip was a prospective, observational, multicenter study, conducted at 6 Stroke Centers in Catalonia. Consecutive patients with suspected stroke were enrolled within the first 6 hours after symptom onset, and blood samples were drawn immediately after admission...
July 17, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28716758/oral-delivery-of-vancomycin-by-tetraether-lipid-liposomes
#8
Philipp Uhl, Silvia Pantze, Philip Storck, Johannes Parmentier, Dominik Witzigmann, Götz Hofhaus, Jörg Huwyler, Walter Mier, Gert Fricker
Despite the outstanding progress in modern medicine, the oral delivery of peptide drugs is limited until today due to their instability in the gastrointestinal tract and low mucosa penetration. To overcome these hurdles, liposomes containing the specific tetraether lipid GCTE (glycerylcaldityltetraether lipid) were examined. For this purpose, the glycopeptide antibiotic vancomycin was used as model substance and liposomes were prepared by DAC (dual assymetric centrifugation). These liposomes showed a size and polydispersity index comparable to standard liposomes...
July 14, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28716658/stool-antigen-immunodetection-for-diagnosis-of-giardia-duodenalis-infection-in-human-subjects-with-hiv-and-cancer
#9
Maryam Nooshadokht, Behjat Kalantari-Khandani, Iraj Sharifi, Hossein Kamyabi, Namal P M Liyanage, Laurel A Lagenaur, Martin F Kagnoff, Steven M Singer, Zahra Babaei, Shahram Solaymani-Mohammadi
Human infection with the protozoan parasite Giardia duodenalis is one the most common parasitic diseases worldwide. Higher incidence rates of giardiasis have been reported from human subjects with multiple debilitating chronic conditions, including hypogammaglobulinemia and common variable immunodeficiency (CVID). In the current study, stool specimens were collected from 199 individuals diagnosed with HIV or cancer and immunocompetent subjects. The sensitivity of microscopy-based detection on fresh stool preparations, trichrome staining and stool antigen immunodetection for the diagnosis of G...
July 14, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28716657/evaluation-of-sofia-fluorescent-immunoassay-analyzer-for-pneumococcal-urinary-antigen-detection-in-hospitalized-patients-with-community-acquired-pneumonia
#10
Diego Vicente, Maddi López-Olaizola, Idoia de la Caba, Gustavo Cilla
The Sofia Streptococcus pneumoniae FIA® test was prospectively evaluated in non-concentrated urine samples of 106 hospitalized patients with community-acquired pneumonia. The test detected pneumococcal urinary antigen in 24/31 (77.4%) confirmed pneumococcal community-acquired pneumonia episodes. The specificity of the test was 86.7% (92% after urine heating).
July 14, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28716138/prevalence-of-enteric-infections-among-hospitalized-patients-in-two-referral-hospitals-in-ghana
#11
R Akuffo, G Armah, M Clemens, K C Kronmann, A H Jones, P Agbenohevi, K Sagoe, N Puplampu, N Talla Nzussouo, W Ampofo, K Koram, C Duplessis, E Dueger
BACKGROUND: Diarrhea is an important cause of morbidity and mortality worldwide. In Africa and Ghana in particular, it is estimated to contribute directly to 19 and 25% of pediatric mortality among children under 5 years, respectively. METHODS: Surveillance for hospitalized acute diarrheal illness was initiated in November 2010 through October 2012 in a referral hospital in southern Ghana, and a teaching hospital in northern Ghana. Consenting hospitalized patients who met a standardized case definition for acute diarrheal illness provided demographic and epidemiologic data...
July 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28715494/development-and-validation-of-novel-biomarker-assays-for-osteoarthritis
#12
Khadija Ourradi, Yunhe Xu, Dominique de Seny, John Kirwan, Ashley Blom, Mohammed Sharif
BACKGROUND: Osteoarthritis (OA) is the most common chronic joint disease usually diagnosed at relatively advanced stages when there is irreparable damage to the joint(s). Recently, we have identified two novel biomarkers C3f and V65 which appear to be OA-specific and therefore potential markers of early disease. We report the development of immunoassays for quantitative measure of these two novel biomarkers. METHOD: Monoclonal and polyclonal antibodies were generated by immunising mouse and rabbits respectively with peptide-carrier conjugates of C3f and V65...
2017: PloS One
https://www.readbyqxmd.com/read/28715465/multiplexing-detection-of-igg-against-plasmodium-falciparum-pregnancy-specific-antigens
#13
Ana Maria Fonseca, Llorenç Quinto, Alfons Jiménez, Raquel González, Azucena Bardají, Sonia Maculuve, Carlota Dobaño, Maria Rupérez, Anifa Vala, John J Aponte, Esperanza Sevene, Eusebio Macete, Clara Menéndez, Alfredo Mayor
BACKGROUND: Pregnant women exposed to Plasmodium falciparum generate antibodies against VAR2CSA, the parasite protein that mediates adhesion of infected erythrocytes to the placenta. There is a need of high-throughput tools to determine the fine specificity of these antibodies that can be used to identify immune correlates of protection and exposure. Here we aimed at developing a multiplex-immunoassay to detect antibodies against VAR2CSA antigens. METHODS AND FINDINGS: We constructed two multiplex-bead arrays, one composed of 3 VAR2CSA recombinant-domains (DBL3X, DBL5Ɛ and DBL6Ɛ) and another composed of 46 new peptides covering VAR2CSA conserved and semi-conserved regions...
2017: PloS One
https://www.readbyqxmd.com/read/28714736/does-music-therapy-improve-anxiety-and-depression-in-alzheimer-s-patients
#14
José Enrique de la Rubia Ortí, María Pilar García-Pardo, Carmen Cabañés Iranzo, José Joaquin Cerón Madrigal, Sandra Sancho Castillo, Mariano Julián Rochina, Vicente Javier Prado Gascó
OBJECTIVE: To evaluate the effectiveness of the implementation of a short protocol of music therapy as a tool to reduce stress and improve the emotional state in patients with mild Alzheimer's disease. METHODS: A sample of 25 patients with mild Alzheimer's received therapy based on the application of a music therapy session lasting 60 min. Before and after the therapy, patient saliva was collected to quantify the level of salivary cortisol using the Enzyme-Linked ImmunoSorbent Assay (ELISA) immunoassay technique and a questionnaire was completed to measure anxiety and depression (Hospital Anxiety and Depression Scale)...
July 17, 2017: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/28714420/evaluation-of-temporal-changes-in-urine-based-metabolomic-and-kidney-injury-markers-to-detect-compound-induced-acute-kidney-tubular-toxicity-in-beagle-dogs
#15
M P Wagoner, Y Yang, J E McDuffie, M Klapczynski, W Buck, L Cheatham, D Eisinger, F Sace, K M Lynch, M Sonee, J-Y Ma, Y Chen, K Marshall, M Damour, L Stephen, Y P Dragan, J Fikes, S Snook, L B Kinter
Urinary protein biomarkers and metabolomic markers have been leveraged to detect acute drug induced kidney injury (DIKI) in rats; however, the utility of these indicators to enable early detection of DIKI in canine models has not been well documented. Therefore, we evaluated temporal changes in biomarkers and metabolites in urine from male and female beagle dogs. Gentamicin-induced kidney lesions in male dogs were characterized by moderate to severe tubular epithelial cell degeneration/necrosis, epithelial cell regeneration and dilation; and a unique urine-based metabolomic fingerprint...
July 13, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28712952/a-robust-high-throughput-fungal-biosensor-assay-for-the-detection-of-estrogen-activity
#16
Christoph Zutz, Karen Wagener, Desislava Yankova, Stefanie Eder, Erich Möstl, Marc Drillich, Kathrin Rychli, Martin Wagner, Joseph Strauss
Estrogenic active compounds are present in a variety of sources and may alter biological functions in vertebrates. Therefore, it is crucial to develop innovative analytical systems that allow us to screen a broad spectrum of matrices and deliver fast and reliable results. We present the adaptation and validation of a fungal biosensor for the detection of estrogen activity in cow derived samples and tested the clinical applicability for pregnancy diagnosis in 140 mares and 120 cows. As biosensor we used a previously engineered genetically modified strain of the filamentous fungus Aspergillus nidulans, which contains the human estrogen receptor alpha and a reporter construct, in which β-galactosidase gene expression is controlled by an estrogen-responsive-element...
July 13, 2017: Steroids
https://www.readbyqxmd.com/read/28712550/towards-an-automatic-lab-on-valve-ion-mobility-spectrometric-system-for-detection-of-cocaine-abuse
#17
David J Cocovi-Solberg, Francesc A Esteve-Turrillas, Sergio Armenta, Miguel de la Guardia, Manuel Miró
A lab-on-valve miniaturized system integrating on-line disposable micro-solid phase extraction has been interfaced with ion mobility spectrometry for the accurate and sensitive determination of cocaine and ecgonine methyl ester in oral fluids. The method is based on the automatic loading of 500μL of oral fluid along with the retention of target analytes and matrix clean-up by mixed-mode cationic/reversed-phase solid phase beads, followed by elution with 100μL of 2-propanol containing (3% v/v) ammonia, which are online injected into the IMS...
June 30, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28712183/association-of-abo-and-rh-blood-group-types-to-hepatitis-b-hepatitis-c-hiv-and-syphilis-infection-a-five-year-experience-in-healthy-blood-donors-in-a-tertiary-care-hospital
#18
Zufishan Batool, Saadia Haroon Durrani, Shazia Tariq
BACKGROUND: Aim of the study: The aim of the study was to find out the frequency of Hepatitis B Hepatitis C, Syphilis, HIV and malaria in apparently healthy blood donors and to find out any association between ABO and Rh blood groups. METHODS: It was a descriptive study carried out at Rehman Medical Institute laboratory. All blood donors who volunteered for blood donation from Jan 2008 to Dec 2014 were reviewed for blood groups and screening tests. Those who were eligible were then screened for Hepatitis B, Hepatitis C, HIV, syphilis and malaria on Architect 8200i through chemiluminescent immunoassay whereas malaria was screened by a thin film...
January 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28711239/short-communication-correlation-between-within-herd-antibody-prevalence-and-bulk-tank-milk-antibody-levels-to-mycobacterium-avium-ssp-paratuberculosis-using-2-commercial-immunoassays
#19
M N Pesqueira, E Yus, C Factor, I Mato, M L Sanjuán, C Eiras, I Arnaiz, F J Diéguez
The objective of this study was to determine the correlation between the results obtained with the ELISA technique for antibodies to Mycobacterium avium ssp. paratuberculosis in serum and bulk tank milk at the herd level. For this purpose, 203 samples of bulk tank milk were analyzed with 2 commercial ELISA from dairy herds with a prevalence of seropositive animals that was also determined. In regard to the reference test (results in blood serum), the sensitivity of the bulk tank milk test to detect high-positive herds (≥10% seroprevalence) ranged from 85...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28710906/upward-drift-in-cerebrospinal-fluid-amyloid-%C3%AE-42-assay-values-for-more-than-10%C3%A2-years
#20
Suzanne E Schindler, Courtney L Sutphen, Charlotte Teunissen, Lena M McCue, John C Morris, David M Holtzman, Sandra D Mulder, Philip Scheltens, Chengjie Xiong, Anne M Fagan
INTRODUCTION: The best-established cerebrospinal fluid (CSF) biomarkers for Alzheimer's disease are levels of amyloid-β 42 (Aβ42), total tau (tau), and phosphorylated tau 181 (ptau). We examined whether a widely used commercial immunoassay for CSF Aβ42, tau, and ptau provided stable measurements for more than ∼10 years. METHODS: INNOTEST assay values for CSF Aβ42, tau, and ptau from Washington University in St. Louis and VU Medical Center, Amsterdam, were evaluated...
July 12, 2017: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
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