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https://www.readbyqxmd.com/read/29679420/a-dual-label-time-resolved-fluorescence-immunoassay-for-the-simultaneous-determination-of-carcino-embryonic-antigen-and-squamous-cell-carcinoma-antigen
#1
Qian Mao, Xiaohong Liu, Jingwen Ye, Huankun Liang, Binxian Li, Xinyi Sun, Lin Sun, Yuman Sun, Xiaomei Mu, Laiqing Li
Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcino-embryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in the serum were captured by anti-CEA and anti-SCC antibodies. After combining other anti-CEA- and anti-SCC-labeled antibodies with europium (III) (Eu3+ ) and samarium (III) (Sm3+ ) chelates, CEA and SCC were detected with time-resolved fluorometry (TRF)...
April 20, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29679392/comparison-of-electrochemiluminescence-immunoassay-and-latex-agglutination-turbidimetric-immunoassay-for-evaluation-of-everolimus-blood-concentrations-in-renal-transplant-patients
#2
Y Akamine, S Sato, H Kagaya, T Ohkubo, S Satoh, M Miura
WHAT IS KNOWN AND OBJECTIVE: For analysis of blood concentrations of everolimus, many hospital laboratories use either latex agglutination turbidimetric immunoassay (LTIA) or electrochemiluminescence immunoassay (ECLIA). However, no studies have compared both immunoassay methods under the same conditions. Accordingly, in this study, we compared everolimus blood concentrations obtained by LTIA and ECLIA in renal transplant patients. METHODS: Blood samples (n = 230) from 60 renal transplant patients (19 female and 41 male) were evaluated using both immunoassays...
April 20, 2018: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/29679387/the-pharmacokinetics-and-pharmacodynamics-of-carfentanil-after-recreational-exposure-a-case-report
#3
Uvaraj Uddayasankar, Colin Lee, Curtis Oleschuk, Gregg Eschun, Robert E Ariano
Carfentanil and related fentanyl analogues have been linked to a number of overdose deaths from drug users in several cities across North America. Diagnosis of carfentanil exposure requires a very high index of clinical suspicion, especially because available laboratory narcotic screens do not detect this agent. We describe a 34-year-old male admitted with depressed level of consciousness and in respiratory failure after recreational exposure to a white powder later inferred to contain carfentanil. Urine and whole blood samples were obtained for conventional preliminary drug screen immunoassays for unknown exposures; in addition to utilizing high pressure liquid chromatography tandem mass spectrometry assay for quantification of carfentanil and its metabolite...
April 20, 2018: Pharmacotherapy
https://www.readbyqxmd.com/read/29678842/biotin-at-high-concentration-interferes-with-the-loci-digoxin-assay-but-the-petinia-phenytoin-assay-is-not-affected
#4
Joe J Rodriguez, Fernando Acosta, Lavern Bourgeois, Amitava Dasgupta
Many automated immunoassays incorporate biotinylated antibodies and streptavidin-coated magnetic beads in the assay design. Biotin at elevated concentrations may interfere with these immunoassays. We evaluated potential interference of biotin on serum digoxin (LOCI assay utilizing biotinylated antibody) and phenytoin (PETINIA assay; no biotinylated antibody) measurements using the Vista 1500 analyzer. Aliquots of drug-free serum pool were supplemented with various biotin concentrations (range: 1 ng/mL to 250 ng/mL) followed by measuring apparent digoxin and phenytoin levels using appropriate immunoassays...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29678795/a-novel-platform-for-in-vivo-detection-of-cytokine-release-within-discrete-brain-regions
#5
Kaixin Zhang, Michael V Baratta, Guozhen Liu, Matthew G Frank, Nathan R Leslie, Linda R Watkins, Steven F Maier, Mark R Hutchinson, Ewa M Goldys
Mounting evidence indicates that cytokines secreted by innate immune cells in the brain play a central role in regulating neural circuits that subserve mood, cognition, and sickness responses. A major impediment to the study of neuroimmune signaling in healthy and disease states is the absence of tools for in vivo detection of cytokine release in the brain. Here we describe the design and application of a cytokine detection device capable of serial monitoring of local cytokine release in discrete brain regions...
April 17, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29678393/positive-correlations-between-free-vitamin-d-and-bone-variables-in-a-group-of-young-lebanese-women
#6
Abir Alwan, Maroun Rizkallah, Ghassan Maalouf, Joseph Matta, Fabienne Frenn, Angélique Barakat, Falah Bachour, Amer Sebaaly, Abdel-Jalil Berro, Nathalie Al Rassy, Mirza Howayek, Hassane Zouhal, Rawad El Hage
Optimizing bone mass in adulthood is of great importance to prevent the occurrence of osteoporosis in later age. Vitamin D is an essential component of bone health. Low-serum vitamin D is associated with low bone mineral density (BMD), which is an important predictor of fracture risk. However, most cells, apart from renal tubular cells, are exposed to free rather than to total 25-hydroxyvitamin D. Whether free vitamin D would be a better marker than total vitamin D is still under debate. The aim of the present study was to explore the relationships between serum total vitamin D, vitamin D-binding protein (BP), free vitamin D, and bone parameters in a group of young Lebanese women...
March 26, 2018: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/29677049/urinary-podocyte-associated-molecules-and-albuminuria-in-hypertension
#7
Javier Perez-Hernandez, Maria D Olivares, Elena Solaz, Fernando Martinez, Sergio Martínez-Hervas, Gernot Pichler, Felipe J Chaves, Josep Redon, Raquel Cortes
OBJECTIVE: Hypertension-induced podocyte damage and the relationship with UAE is analyzed in diabetic and nondiabetic participants. PATIENTS AND METHODS: Sixty-four hypertensive patients, 30 diabetics, with glomerular filtration rate (eGFR) greater than 60 ml/min per 1.73 m were included. Urinary albumin excretion was measured in morning urine using a nephelometric immunoassay and expressed as albumin/creatinine ratio. Urinary pellets were obtained from fresh urine and mRNA was assessed by real-time quantitative PCR...
April 19, 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29677048/criteria-for-diagnosing-primary-aldosteronism-on-the-basis-of-liquid-chromatography-tandem-mass-spectrometry-determinations-of-plasma-aldosterone-concentration
#8
Stephanie Baron, Laurence Amar, Anne-Laure Faucon, Anne Blanchard, Laurence Baffalie, Catherine Faucard, Simon Travers, Jean-Yves Pagny, Michel Azizi, Pascal Houillier
BACKGROUND: Primary aldosteronism is affecting about 10% of hypertensive patients. Primary aldosteronism should be diagnosed by screening tests based on plasma aldosterone concentration (PAC) and aldosterone-to-renin ratio (ARR), followed by confirmatory test. The cutoff values for PAC and ARR depend on PAC and plasma renin measurement methods. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), the new gold standard method for aldosterone determination, is now widespread but shows lower values than immunoassays...
April 19, 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29675893/characteristics-of-opioid-maintained-clients-smoking-fentanyl-patches-the-importance-of-confirmatory-drug-analysis-illustrated-by-a-case-series-and-mini-review
#9
Andreas Kimergård, Jacklyn Dunne, Anette Bøgen, Peter Hindersson, Torben Breindahl
The increase in opioid prescribing in many European countries over the last decade has raised concerns about associated diversion, overdose and mortality. Fentanyl is one of these synthetic opioids that is typically prescribed as a transdermal patch for pain that requires continuous pain relief and has been the focus of investigations due to reports of overdose and death. We report a case series of 14 drug addiction treatment entrants who entered treatment in one service located in the region of Southern Denmark from August 2015 to December 2015 for smoking fentanyl patches...
April 19, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29675793/no-influence-of-smoking-status-on-the-performance-of-urine-markers-for-the-detection-of-bladder-cancer
#10
Susanne Deininger, J Hennenlotter, S Rausch, K Docktor, E Neumann, I A da Costa, J Bedke, A Stenzl, T Todenhöfer
PURPOSE: The performance of urinary markers for detecting bladder cancer (BC) is influenced by various factors. The aim of the present study was to evaluate the influence of smoking habits on the performance of four commonly used urine markers. METHODS: Urine samples of 723 patients with suspected BC were analysed using urine cytology, fluorescence in situ hybridization (FISH), immunocytology (uCyt+ test), and quantitative nuclear matrix protein 22 (NMP22) immunoassay...
April 19, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29675559/truncated-aptamers-for-total-and-glycated-hemoglobin-and-their-integration-into-a-graphene-oxide-based-fluorometric-method-for-high-throughput-screening-for-diabetes
#11
Abrar Yousef Almusharraf, Shimaa Eissa, Mohammed Zourob
The authors describe the identification of an effective binding region of aptamers against glycated (HbA1c) and total haemoglobin (tHb) by using a fluorometric assay. Truncation of the originally selected aptamers from 60 to 46 and 34 nucleotides for HbA1c and tHb, respectively, enhances the affinity for their targets. Moreover, shortening the aptamer sequences leads to a better conformational change after target binding which enabled the integration of the aptamers in a graphene oxide (GO)-based fluorometric assay...
April 19, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29674713/enzyme-catalyzed-ag-growth-on-au-nanoparticle-assembled-structure-for-highly-sensitive-colorimetric-immunoassay
#12
Xuan-Hung Pham, Eunil Hahm, Tae Han Kim, Hyung-Mo Kim, Sang Hun Lee, Yoon-Sik Lee, Dae Hong Jeong, Bong-Hyun Jun
We have developed a sensitive colorimetric immunoassay with broad dynamic range using enzyme-catalyzed Ag growth on gold nanoparticle (NP)-assembled silica (SiO2 @Au@Ag). To reduce Ag+ ion content and promote Ag growth on the assembled Au NPs, alkaline phosphatase (AP)-based enzymatic amplification was incorporated, which considerably increased the colorimetric read-out. As a model study, sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantify target IgG. The immune complexes capture the Ab-IgG-AP-labeled detection Ab and trigger the enzyme-catalyzed reaction to convert 2-phospho-L-ascorbic acid to ascorbic acid in the presence of the target IgG...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29674647/oxygen-doped-carbon-nanotubes-for-near-infrared-fluorescent-labels-and-imaging-probes
#13
Yoko Iizumi, Masako Yudasaka, Jaeho Kim, Hajime Sakakita, Tsukasa Takeuchi, Toshiya Okazaki
Chemical modification of carbon nanotube surface can controllably modulate their optical properties. Here we report a simple and effective synthesis method of oxygen-doped single-walled carbon nanotubes (o-SWCNTs), in which a thin film of SWCNTs is just irradiated under the UV light for a few minutes in air. By using this method, the epoxide-type oxygen-adducts (ep-SWCNTs) were produced in addition to the ether-type oxygen-adducts (eth-SWCNTs). The Treated (6, 5) ep-SWCNTs show a red-shifted luminescence at ~1280 nm, which corresponds to the most transparent regions for bio-materials...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29674208/increased-serum-levels-of-sflt-1-plgf-ratio-in-preeclamptic-women-with-onset-at-32-weeks-compared-with-%C3%A2-32-weeks
#14
Hirotada Suzuki, Chikako Hirashima, Shiho Nagayama, Kayo Takahashi, Tatsuo Yamamoto, Shigeki Matsubara, Akihide Ohkuchi
OBJECTIVE: Our first aim was to construct gestational-age-specific reference ranges of serum levels of soluble fms-like tyrosine kinase 1 (sFlt-1), placental growth factor (PlGF), and the sFlt-1/PlGF ratio at 19-38 weeks of gestation. Our second aim was to compare the serum levels of sFlt-1, PlGF, and the sFlt-1/PlGF ratio in 81 women with PE which occurred at <32, 32-33, 34-35, and ≥36 weeks. METHOD: Serum levels of sFlt-1 and PlGF were measured by automated immunoassays (Elecsys sFlt-1 and Elecsys PlGF)...
March 19, 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29674187/lower-maternal-serum-tumor-necrosis-factor-related-apoptosis-inducing-ligand-trail-levels-in-early-preeclampsia-a-retrospective-study
#15
Danila Morano, Alessandro Rolfo, Veronica Tisato, Antonio Farina, Erika Rimondi, Gennaro Scutiero, Pantaleo Greco, Gloria Bonaccorsi, Tullia Todros
OBJECTIVE: To determine whether maternal serum concentrations of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a cytokine with anti-inflammatory activity, also involved in cardiovascular morbidity, differ between women with early preeclampsia (<34 weeks) and those with uncomplicated pregnancies. STUDY DESIGN: This nested case control study included 40 women carrying a single fetus with an uncomplicated pregnancy and 20 women with early preeclampsia (<34 weeks)...
February 7, 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29673814/assessment-of-hair-cortisol-as-a-potential-biomarker-for-possible-adrenal-suppression-due-to-inhaled-corticosteroid-use-in-children-with-asthma-a-retrospective-observational-study
#16
Laura Smy, Kaitlyn Shaw, Ursula Amstutz, Michelle Staub, Shahnaz Chaudhry, Anne Smith, Bruce Carleton, Gideon Koren
BACKGROUND: Inhaled corticosteroids (ICS) are the recommended long-term control therapy for asthma in children. However, concern exists regarding potential adrenal suppression with chronic ICS use. Our pilot study reported that hair cortisol in children was 50% lower during ICS therapy than prior to therapy, suggestive of adrenal suppression. OBJECTIVE: To evaluate hair cortisol concentration (HCC) as a potential biomarker for possible adrenal suppression from ICS use in children with asthma...
April 16, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29672552/induction-of-salivary-antibody-levels-in-dutch-adolescents-after-immunization-with-monovalent-meningococcal-serogroup-c-or-quadrivalent-meningococcal-serogroup-a-c-w-and-y-conjugate-vaccine
#17
Mariëtte B van Ravenhorst, Gerco den Hartog, Fiona R M van der Klis, Debbie M van Rooijen, Elisabeth A M Sanders, Guy A M Berbers
BACKGROUND: Meningococcal infection starts with colonisation of the upper respiratory tract. Mucosal immunity is important for protection against acquisition and subsequent meningococcal carriage. In this study, we assessed salivary antibody levels against meningococcal serogroup A (MenA), W (MenW) and Y (MenY) after vaccination with a quadrivalent MenACWY conjugated vaccine. We also compared salivary meningococcal serogroup C (MenC) antibody levels after monovalent MenC and quadrivalent MenACWY conjugated vaccination...
2018: PloS One
https://www.readbyqxmd.com/read/29672339/the-effects-of-episodic-alcohol-exposure-on-bmp2-signaling-during-tibia-fracture-healing
#18
Anthony Bratton, Joshua Eisenberg, Aleksandra Vuchkovska, Philip Roper, John J Callaci
OBJECTIVES: To explore how alcohol affects the BMP-2 signaling pathway, which is known to play a critical role in bone and cartilage formation during fracture healing. METHODS: A rat model was used to demonstrate the detrimental effects of alcohol exposure on tibia fracture healing. Specific components of the BMP-2 pathway were analyzed in fracture callus at days 3, 7, 14, 21 post fracture via western immunoassays and ELISA. RESULTS: Alcohol exposure prior to tibia fracture demonstrated attenuation of downstream BMP-2 signaling...
April 18, 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29671898/serum-levetiracetam-concentrations-and-adverse-events-after-multiple-dose-extended-release-levetiracetam-administration-to-healthy-cats
#19
Heidi Barnes Heller, Martin Granick, Mathew Van Hesteren, Dawn M Boothe
BACKGROUND: Multiple dose administration of antiepileptic drugs to cats presents a challenge for owners. Extended release levetiracetam (XRL) has once daily recommended dosing interval, but multiple dose administration of XRL has not been evaluated in cats. OBJECTIVE: Evaluate serum levetiracetam concentrations and adverse clinical effects after 11 days of once daily XRL administration to healthy cats. ANIMALS: Nine healthy privately owned cats, body weight ≥ 5 kg METHODS: Extended release levetiracetam (500 mg/cat) was administered PO q24h for 10 days...
April 19, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29670608/galactomannan-a-surrogate-marker-for-outcome-in-invasive-aspergillosis-finally-coming-of-age
#20
REVIEW
Toine Mercier, Ellen Guldentops, Katrien Lagrou, Johan Maertens
Detection of galactomannan has become widely available for diagnosing invasive aspergillosis. The test characteristics, using the Platelia™ enzyme-immunoassay, have been well described. This assay could potentially also be useful for the early evaluation of the efficacy of antifungal therapy and for predicting the outcome in terms of response and survival. In this systematic review, we assessed the available evidence for the use of serum galactomannan at baseline as a prognostic marker, and the predictive value of serum galactomannan kinetics after initiation of antifungal therapy...
2018: Frontiers in Microbiology
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