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Maria Parapouli, Athanasios Foukis, Panagiota-Yiolanda Stergiou, Maria Koukouritaki, Panagiotis Magklaras, Olga A Gkini, Emmanuel M Papamichael, Amalia-Sofia Afendra, Efstathios Hatziloukas
Background: Microbial lipases catalyze a broad spectrum of reactions and are enzymes of considerable biotechnological interest. The focus of this study was the isolation of new lipase genes, intending to discover novel lipases whose products bear interesting biochemical and structural features and may have a potential to act as valuable biocatalysts in industrial applications. Results: A novel lipase gene ( lipSm ), from a new environmental Stenotrophomonas maltophilia strain, Psi-1, originating from a sludge sample from Psittaleia (Greece), was cloned and sequenced...
December 2018: Journal of Biological Research
Davide Cucchi, Alessandra Menon, Riccardo Compagnoni, Paolo Ferrua, Chiara Fossati, Pietro Randelli
PURPOSE: Patient-specific instrumentation (PSI) for total knee arthroplasty (TKA) may improve component sizing. Little has been reported about accuracy of the default plan created by the manufacturer, especially for CT-based PSI. The goal of this study was to evaluate the reliability of this plan and the impact of the surgeon's changes on the final accuracy of the guide sizes. METHODS: Forty-five patients eligible for primary TKA were prospectively enrolled. The planned implant sizes were prospectively recorded from the initial manufacturer's proposal and from the final plan adjusted in light of the surgeon's evaluation; these two sizes where then compared to the actually implanted sizes...
February 16, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
M H Kuizenga, P J Colin, K M E M Reyntjens, D J Touw, H Nalbat, F H Knotnerus, H E M Vereecke, M M R F Struys
BACKGROUND: Neural inertia is defined as the tendency of the central nervous system to resist transitions between arousal states. This phenomenon has been observed in mice and Drosophila anaesthetized with volatile anaesthetics: the effect-site concentration required to induce anaesthesia in 50% of the population (C50 ) was significantly higher than the effect-site concentration for 50% of the population to recover from anaesthesia. We evaluated this phenomenon in humans using propofol or sevoflurane (both with or without remifentanil) as anaesthetic agents...
March 2018: British Journal of Anaesthesia
Marlena H Shin, Peter E Rivard, Michael Shwartz, Ann Borzecki, Enzo Yaksic, Kelly Stolzmann, Lisa Zubkoff, Amy K Rosen
BACKGROUND: Given that patient safety measures are increasingly used for public reporting and pay-for performance, it is important for stakeholders to understand how to use these measures for improvement. The Agency for Healthcare Research and Quality (AHRQ) Patient Safety Indicators (PSIs) are one particularly visible set of measures that are now used primarily for public reporting and pay-for-performance among both private sector and Veterans Health Administration (VA) hospitals. This trend generates a strong need for stakeholders to understand how to interpret and use the PSIs for quality improvement (QI)...
February 14, 2018: BMC Health Services Research
Young Sung Kim, Dongik Chung, Seok Kyeong Oh, Young Ju Won, Il Ok Lee
BACKGROUND: EEG monitoring is useful for determining an adequate level of anesthesia. However it is sometimes interfered by various reasons. We describe a case in which we successfully confirmed the adequate depth of anesthesia by monitoring the patient state index (PSI), which was computed from the SedLine monitor data in Root (Masimo) during general anesthesia. Our case showed unusual elevations in entropy, but not in PSI. CASE PRESENTATION: A 34-year-old woman was scheduled for emergency surgery for a left tibial open fracture and a right femoral closed fracture, which were sustained during a traffic accident...
February 14, 2018: BMC Anesthesiology
Ane Uranga, Jose M Quintana, Urko Aguirre, Amaia Artaraz, Rosa Diez, Silvia Pascual, Aitor Ballaz, Pedro P España
BACKGROUND: Community-acquired pneumonia (CAP) is a major public health problem with high short- and long-term mortality. The main aim of this study was to develop and validate a specific prognostic index for one-year mortality in patients admitted for CAP. METHODS: This was an observational, prospective study of adults aged ≥18 years admitted to Galdakao-Usansolo Hospital (Bizkaia, Spain) from January 2001 to July 2009 with a diagnosis of CAP surviving the first 15 days...
2018: PloS One
Fumiyoshi Myouga, Kaori Takahashi, Ryouichi Tanaka, Noriko Nagata, Anett Z Kiss, Christiane Funk, Yuko Nomura, Hirofumi Nakagami, Stefan Jansson, Kazuo Shinozaki
The cellular functions of two Arabidopsis one-helix proteins, OHP1 and OHP2 [also named light-harvesting-like Lil2 and Lil6, respectively, because they have sequence similarity to light-harvesting chlorophyll a/b-binding (LHC) proteins] remain unclear. Tagged null mutants of OHP1 and OHP2 (ohp1 and ohp2) showed stunted growth with pale-green leaves on agar plates, and these mutants were unable to grow on soil. Leaf chlorophyll fluorescence and the composition of thylakoid membrane proteins revealed that ohp1 deletion significantly affected photosystem II (PSII) core protein function and led to reduced levels of PSI core proteins; however, it did not affect LHC accumulation...
February 1, 2018: Plant Physiology
Zhi-Yan Du, Ben Franklin Lucker, Krzysztof Zienkiewicz, Tarryn E Miller, Agnieszka Zienkiewicz, Barbara B Sears, David M Kramer, Christoph Benning
Photosynthesis occurs in the thylakoid membrane, where the predominant lipid is monogalactosyldiacylglycerol (MGDG). As environmental conditions change, photosynthetic membranes have to adjust. In this study, we used a loss-of-function Chlamydomonas reinhardtii mutant deficient in the MGDG-specific lipase PGD1 (PLASTID GALACTOGLYCEROLIPID DEGRADATION1) to investigate the link between MGDG turnover, chloroplast ultrastructure, and the production of reactive oxygen species (ROS) in response to different adverse environmental conditions...
February 5, 2018: Plant Cell
Norbert E Juettner, Stefan Schmelz, Jan P Bogen, Dominic Happel, Wolf-Dieter Fessner, Felicitas Pfeifer, Hans-Lothar Fuchsbauer, Andrea Scrima
Transglutaminase from Streptomyces mobaraensis (MTG) has become a powerful tool to covalently and highly specifically link functional amines to glutamine donor sites of therapeutic proteins. However, details regarding the mechanism of substrate recognition and interaction of the enzyme with proteinaceous substrates still remain mostly elusive. We have determined the crystal structure of the Streptomyces papain inhibitory protein (SPIp ), a substrate of MTG, to study the influence of various substrate amino acids on positioning glutamine to the active site of MTG...
February 12, 2018: Protein Science: a Publication of the Protein Society
Ji-Hyeon Oh
With the development of computer-aided design/computer-aided manufacturing (CAD/CAM) technology, it has been possible to reconstruct the cranio-maxillofacial defect with more accurate preoperative planning, precise patient-specific implants (PSIs), and shorter operation times. The manufacturing processes include subtractive manufacturing and additive manufacturing and should be selected in consideration of the material type, available technology, post-processing, accuracy, lead time, properties, and surface quality...
December 2018: Maxillofacial Plastic and Reconstructive Surgery
María Eugenia Frey, Sebastián D'Ippolito, Alfonso Pepe, Gustavo Raúl Daleo, María Gabriela Guevara
The plant-specific insert of Solanum tuberosum aspartic proteases (StAP-PSI) has high structural similarity with NK-lysin and granulysin, two saposin-like proteins (SAPLIPs) with antimicrobial activity. Recombinant StAP-PSI and some SAPLIPs show antimicrobial activity against pathogens that affect human and plants. In this work, we transformed Arabidopsis thaliana plants with StAP-PSI encoding sequence with its corresponding signal peptide under the control of the cauliflower mosaic virus (CaMV) 35S promoter...
February 8, 2018: Phytochemistry
Melinda Magyar, Gábor Sipka, László Kovács, Bettina Ughy, Qingjun Zhu, Guangye Han, Vladimír Špunda, Petar H Lambrev, Jian-Ren Shen, Győző Garab
Photosystem II (PSII) catalyses the photoinduced oxygen evolution and, by producing reducing equivalents drives, in concert with PSI, the conversion of carbon dioxide to sugars. Our knowledge about the architecture of the reaction centre (RC) complex and the mechanisms of charge separation and stabilisation is well advanced. However, our understanding of the processes associated with the functioning of RC is incomplete: the photochemical activity of PSII is routinely monitored by chlorophyll-a fluorescence induction but the presently available data are not free of controversy...
February 9, 2018: Scientific Reports
Yoshifumi Ueno, Ginga Shimakawa, Chikahiro Miyake, Seiji Akimoto
To maximize the efficiency of photosynthesis, photosynthetic organisms must properly balance their light-harvesting ability and CO2 utilization. However, the molecular mechanisms of light harvesting under various CO2 conditions remain unclear. To reveal these mechanisms, we performed a new analysis on cells of the green alga Chlamydomonas reinhardtii under different CO2 conditions. The analysis combines three kinds of fluorometries: pulse-amplitude modulated fluorescence, steady-state fluorescence with absolute intensity, and time-resolved fluorescence...
February 9, 2018: Journal of Physical Chemistry Letters
Sung In Jeong, Seong-Cheol Park, Sun-Jeong Park, Eun-Ji Kim, Hun Heo, Jong-Seok Park, Hui-Jeong Gwon, Youn-Mook Lim, Mi-Kyeong Jang
Introduction: Although numerous studies have been conducted with the aim of developing drug-delivery systems, chemically synthesized gene carriers have shown limited applications in the biomedical fields due to several problems, such as low-grafting yields, undesirable reactions, difficulties in controlling the reactions, and high-cost production owing to multi-step manufacturing processes. Materials and methods: We developed a 1-step synthesis process to produce 2-aminoethyl methacrylate-grafted water-soluble chitosan (AEMA-g-WSC) as a gene carrier, using gamma irradiation for simultaneous synthesis and sterilization, but no catalysts or photoinitiators...
2018: International Journal of Nanomedicine
Tirupathi Malavath, Ido Caspy, Sigal Y Netzer-El, Daniel Klaiman, Nathan Nelson
The ability of photosynthetic organisms to use the sun's light as a sole source of energy sustains life on our planet. Photosystems I (PSI) and II (PSII) are large, multi-subunit, pigment-protein complexes that enable photosynthesis, but this intriguing process remains to be explained fully. Currently, crystal structures of these complexes are available for thermophilic prokaryotic cyanobacteria. The mega-Dalton trimeric PSI complex from thermophilic cyanobacterium, Thermosynechococcus elongatus, was solved at 2...
February 4, 2018: Biochimica et Biophysica Acta
Rodolfo Abreu, Alberto Leal, Fernando Lopes da Silva, Patrícia Figueiredo
OBJECTIVE: We hypothesize that the hypersynchronization associated with epileptic activity is best described by EEG synchronization measures, and propose to use these as predictors of epilepsy-related BOLD fluctuations. METHODS: We computed the phase synchronization index (PSI) and global field synchronization (GFS), within two frequency bands, a broadband (1-45 Hz) and a narrower band focused on the presence of epileptic activity (3-10 Hz). The associated epileptic networks were compared with those obtained using conventional unitary regressors and two power-weighted metrics (total power and root mean square frequency), on nine simultaneous EEG-fMRI datasets from four epilepsy patients, exhibiting inter-ictal epileptiform discharges (IEDs)...
January 11, 2018: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
Ravi Pamu, Venkatanarayana Prasad Sandireddy, Ramki Kalyanaraman, Bamin Khomami, Dibyendu Mukherjee
Plasmonic metal nanostructures have been known to tune optoelectronic properties of fluorophores. To this end, we report the first-ever experimental observation of plasmon-induced photocurrent enhancements from Photosystem I (PSI) attached to Fischer patterns of silver nano-pyramids (Ag-NP) whose resonance peaks are tuned to match the PSI absorption peaks at ~450 and ~680 nm. The plasmon enhanced photocurrents indicate enhancement factors of ~6.5 and ~5.8 as compared to PSI assembly on planar Ag substrates for nominal excitation wavelengths of 660 and 470 nm respectively...
February 6, 2018: Journal of Physical Chemistry Letters
Na Sui, Yu Wang, Shanshan Liu, Zhen Yang, Fang Wang, Shubo Wan
Peanut (Arachis hypogaea L.) is one of the five major oilseed crops cultivated worldwide. Salt stress is a common adverse condition for the growth of this crop in many countries and regions. In this study, physiological parameters and transcriptome profiles of peanut seedlings exposed to salt stress (250 mM NaCl for 4 days, S4) and recovery for 3 days (when transferred to standard conditions for 3 days, R3) were analyzed to detect genes associated with salt stress and recovery in peanut. We observed that the quantum yield of PSII electron transport (ΦPSII) and the maximal photochemical efficiency of PSII (Fv/Fm) decreased in S4 compared with the control, and increased in R3 compared with those in S4...
2018: Frontiers in Plant Science
Junyi Wang, Xinjing Gao, Dong Wang, Zhiyong Wang, Zhibo Li, Dong Liu, Lei Xu
OBJECTIVE: To evaluate the diagnostic value of contrast-enhanced ultrasound in acute kidney injury (AKI) caused by sepsis. METHODS: The sepsis patients admitted to intensive care unit of Tianjin Third Central Hospital from January 2015 to June 2017 were enrolled. All of the patients were completed the 6-hour Bundle treatment and the bilateral renal contrast-enhanced ultrasound within 24 hours, and the peak signal intensity (PSI), peak intensity time (PIT), wash internal rate (WIR) and renal function parameters were measured at the same time...
February 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
T Vaillant, J Steelandt, A-L Cordonnier, S Haghighat, P Anract, P Paubel, C Duhamel
OBJECTIVES: Single use patient-specific instruments (PSI) for total knee prosthesis are introduced as a new alternative to conventional ancillaries and computer assisted surgery by improving implants positioning. An exhaustive review was carried out to identify their specific characteristics, with their advantages and disadvantages. METHODS: Medical devices suppliers were consulted in 2015 for reviewing their PSI. Their technical, clinical and economic data were compared...
February 2, 2018: Annales Pharmaceutiques Françaises
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