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https://www.readbyqxmd.com/read/28205062/gallium-ferredoxin-as-a-tool-to-study-the-effects-of-ferredoxin-binding-to-photosystem-i-without-ferredoxin-reduction
#1
Clara Mignée, Risa Mutoh, Anja Krieger-Liszkay, Genji Kurisu, Pierre Sétif
Reduction of ferredoxin by photosystem I (PSI) involves the [4Fe-4S] clusters FA and FB harbored by PsaC, with FB being the direct electron transfer partner of ferredoxin (Fd). Binding of the redox-inactive gallium ferredoxin to PSI was investigated by flash-absorption spectroscopy, studying both the P700(+) decay and the reduction of the native iron Fd in the presence of FdGa. FdGa binding resulted in a faster recombination between P700(+) and (FA, FB)(-), a slower electron escape from (FA, FB)(-) to exogenous acceptors, and a decreased amount of intracomplex FdFe reduction, in accordance with competitive binding between FdFe and FdGa...
February 15, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28199904/cyclic-electron-flow-may-provide-some-protection-against-psii-photoinhibition-in-rice-oryza-sativa-l-leaves-under-heat-stress
#2
Jemaa Essemine, Yi Xiao, Mingnan Qu, Hualing Mi, Xin-Guang Zhu
Previously we have shown that a quick down-regulation in PSI activity compares to that of PSII following short-term heat stress for two rice groups including C4023 and Q4149, studied herein. These accessions were identified to have different natural capacities in driving cyclic electron flow (CEF) around PSI; i.e., low CEF (lcef) and high CEF (hcef) for C4023 and Q4149, respectively. The aim of this study was to investigate whether these two lines have different mechanisms of protecting photosystem II from photodamage under heat stress...
January 30, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28195970/reduction-of-physiological-strain-under-a-hot-and-humid-environment-by-a-hybrid-cooling-vest
#3
Albert P C Chan, Y Yang, Francis K W Wong, Michael C H Yam, Del P Wong, W F Song
Cooling treatment is regarded as one of good practices to provide safe training conditions to athletic trainers in the hot environment. The present study aimed to investigate whether wearing a commercial lightweight and portable hybrid cooling vest that combines air ventilation fans with frozen gel packs was an effective means to reduce participants' body heat strain. In this within-subject repeated measures study, 10 male volunteers participated in two heat-stress trials (one with the cooling vest - COOL condition, and another without - CON condition, in a randomized order) inside a climatic chamber with a controlled ambient temperature 33 °C and relative humidity (RH) 75% on an experimental day...
February 8, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28195025/the-depth-of-fields-managing-focus-in-the-epistemic-subcultures-of-mind-and-brain-science
#4
David Peterson
The 'psy' sciences emerged from the tangled roots of philosophy, physiology, biology and medicine, and these origins have produced heterogeneous fields. Scientists in these areas work in a complex, overlapping ecology of fields that results in the constant co-presence of dissonant theories, methods and research objects. This raises questions regarding how conceptual clarity is maintained. Using the optical metaphor 'depth of field', I show how researchers in all fields marginalize potential threats to routine scientific work by framing them as either too broad and imprecise or too narrow and technical...
February 2017: Social Studies of Science
https://www.readbyqxmd.com/read/28191457/the-copper-transport-associated-protein-ctr4-can-form-prion-like-epigenetic-determinants-in-schizosaccharomyces-pombe
#5
Theodora Sideri, Yoko Yashiroda, David A Ellis, María Rodríguez-López, Minoru Yoshida, Mick F Tuite, Jürg Bähler
Prions are protein-based infectious entities associated with fatal brain diseases in animals, but also modify a range of host-cell phenotypes in the budding yeast, Saccharomyces cerevisiae. Many questions remain about the evolution and biology of prions. Although several functionally distinct prion-forming proteins exist in S. cerevisiae, [HET-s] of Podospora anserina is the only other known fungal prion. Here we investigated prion-like, protein-based epigenetic transmission in the fission yeast Schizosaccharomyces pombe...
January 2017: Microbial Cell
https://www.readbyqxmd.com/read/28188782/lipid-and-carotenoid-cooperation-driven-adaptation-to-light-and-temperature-stress-in-synechocystis-sp-pcc6803
#6
Tomas Zakar, Eva Herman, Sindhujaa Vajravel, Laszlo Kovacs, Jana Knoppová, Josef Komenda, Ildiko Domonkos, Mihaly Kis, Zoltan Gombos, Hajnalka Laczko-Dobos
Polyunsaturated lipids are important components of photosynthetic membranes. Xanthophylls are the main photoprotective agents, can assist in protection against light stress, and are crucial in the recovery from photoinhibition. We generated the xanthophyll- and polyunsaturated lipid-deficient ROAD mutant of Synechocystis sp. PCC6803 (Synechocystis) in order to study the little-known cooperative effects of lipids and carotenoids (Cars). Electron microscopic investigations confirmed that in the absence of xanthophylls the S-layer of the cellular envelope is missing...
February 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28188705/design-construction-and-optimization-of-a-novel-modular-and-scalable-incubation-chamber-for-continuous-viral-inactivation
#7
Raquel Orozco, Linus Amarikwa, Stephanie Parker, Scott Godfrey, Lindsay Hernandez, Chinenye Wachuku, Ben Mai, Brian Song, Shashidhar Hoskatti, Jinkeng Asong, Marcus Fiadeiro, Parviz Shamlou, Cameron Bardliving, Jon Coffman
We designed, built or 3D printed, and screened tubular reactors that minimize axial dispersion to serve as incubation chambers for continuous virus inactivation of biological products. Empirical residence time distribution data were used to derive each tubular design's volume equivalent to a theoretical plate (VETP) values at a various process flow rates. One design, the Jig In a Box (JIB), yielded the lowest VETP, indicating optimal radial mixing and minimal axial dispersion. A minimum residence time (MRT) approach was employed, where the MRT is the minimum time the product spends in the tubular reactor...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28185762/biochemical-control-and-toxicity-for-favorable-and-intermediate-risk-patients-using-real-time-intraoperative-inverse-optimization-prostate-seed-implant-less-is-more
#8
G Shukla, A Sarkar, A Hanlon, E Crockett, H C Chen, J Martelli-Raben, A Glick, B Benge, M Lobis, S Terranova, T Desperito, D Cozzolino, E Kemmerer, F Mourtada, A Raben
PURPOSE: To report the biochemical control rate and clinical outcomes with real-time inverse planning (inverse optimization prostate seed implant [IO-PSI]) for favorable-risk (FR) and intermediate-risk (IR) prostate adenocarcinoma in a community practice setting. This analysis is an extended followup of our initial report, with favorable early biochemical control rate (biochemical nonevidence of disease) of 97% at 4 years. METHODS AND MATERIALS: Three hundred fifty-seven evaluable patients with FR and IR prostate cancer underwent real-time IO-PSI (iodine-125/145 Gy or palladium-103/120 Gy) between 2001 and 2013...
February 6, 2017: Brachytherapy
https://www.readbyqxmd.com/read/28185041/variety-in-excitation-energy-transfer-processes-from-phycobilisomes-to-photosystems-i-and-ii
#9
Yoshifumi Ueno, Shimpei Aikawa, Kyosuke Niwa, Tomoko Abe, Akio Murakami, Akihiko Kondo, Seiji Akimoto
The light-harvesting antennas of oxygenic photosynthetic organisms capture light energy and transfer it to the reaction centers of their photosystems. The light-harvesting antennas of cyanobacteria and red algae, called phycobilisomes (PBSs), supply light energy to both photosystem I (PSI) and photosystem II (PSII). However, the excitation energy transfer processes from PBS to PSI and PSII are not understood in detail. In the present study, the energy transfer processes from PBS to PSs in various cyanobacteria and red algae were examined in vivo by selectively exciting their PSs or PBSs, and measuring the resulting picosecond to nanosecond time-resolved fluorescences...
February 9, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28184910/stromal-loop-of-lhca6-is-responsible-for-the-linker-function-required-for-the-ndh-psi-supercomplex-formation
#10
Takuto Otani, Hiroshi Yamamoto, Toshiharu Shikanai
No abstract text is available yet for this article.
February 10, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28176211/association-between-parenting-stress-and-functional-impairment-among-children-diagnosed-with-neurodevelopmental-disorders
#11
Yasser S Almogbel, Rohit Goyal, Sujit S Sansgiry
The objective of this study was to examine the association between parenting stress and functional impairment among children with Neurodevelopmental Disorder (NDD). A sample of 150 parents of children diagnosed with NDD were recruited from schools that offer special education services. Parents completed a self-administered survey containing the parenting stress index-short form (PSI-SF) scale and the Columbia Impairment Scale. The multiple logistic regression conducted to compare those with clinically significant PSI-SF scores indicated that the risk of parents with clinically significant scores of parenting stress increased 5...
February 8, 2017: Community Mental Health Journal
https://www.readbyqxmd.com/read/28168816/lactate-levels-and-pneumonia-severity-index-are-good-predictors-of-in-hospital-mortality-in-pneumonia
#12
Bulut Demirel
INTRODUCTION: Pneumonia is an inflammatory disease caused by micro-organisms. The pneumonia severity index (PSI) and CURB_65 scores are widely used to predict the mortality risk. We wanted to calculate the value of lactic acid level in predicting the mortality risk. MATERIAL AND METHODS: This prospective cross-sectional study performed in a third level education and research hospital. The pneumonia diagnosis was determined by symptoms, physical examination findings, and radiological findings...
February 7, 2017: Clinical Respiratory Journal
https://www.readbyqxmd.com/read/28168382/molecular-design-expression-and-evaluation-of-pasylated-human-recombinant-erythropoietin-with-enhanced-functional-properties
#13
Mohammad Hossein Hedayati, Dariush Norouzian, Mahdi Aminian, Shahram Teimourian, Reza Ahangari Cohan, Soroush Sardari, M Reza Khorramizadeh
Erythropoietin (EPO) is the principal hormone which, has somewhat short half-life involved in the differentiation and regulation of circulating red blood cells. The present study was carried out to evaluate the capability of a polyethylene glycol mimetic technology as a biological alternative to improve pharmaceutical properties of human recombinant EPO. In silico models of EPO fused to 200 amino acids of proline, alanine, and serine (PAS) were initially generated and assessed by molecular dynamic (MD) simulation...
February 6, 2017: Protein Journal
https://www.readbyqxmd.com/read/28167040/induction-of-cyclic-electron-flow-around-photosystem-i-during-heat-stress-in-grape-leaves
#14
Yongjiang Sun, Qingwei Geng, Yuanpeng Du, Xinghong Yang, Heng Zhai
Photosystem II (PSII) in plants is susceptible to high temperatures. The cyclic electron flow (CEF) around PSI is thought to protect both PSII and PSI from photodamage. However, the underlying physiological mechanisms of the photosynthetic electron transport process and the role of CEF in grape at high temperatures remain unclear. To investigate this issue, we examined the responses of PSII energy distribution, the P700 redox state and CEF to high temperatures in grape leaves. After exposing 'Cabernet Sauvignon' leaves to various temperatures (25, 30, 35, 40 and 45°C) in the light (600μmol photons m(-2)s(-1)) for 4h, the maximum quantum yield of PSII (Fv/Fm) significantly decreased at high temperatures (40 and 45°C), while the maximum photo-oxidizable P700 (Pm) was not affected...
March 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28167024/a-novel-ethylene-responsive-factor-citerf13-plays-a-role-in-photosynthesis-regulation
#15
Xiu-Lan Xie, Xiao-Jian Xia, Sheng Kuang, Xi-Li Zhang, Xue-Ren Yin, Jing-Quan Yu, Kun-Song Chen
Ethylene responsive factors (ERFs) act as critical downstream components of the ethylene signalling pathway in regulating plant development and stress responses. However little is known about its role in regulation of photosynthesis. Here, we identified an ethylene-inducible ERF gene in citrus, CitERF13. Transient over-expression of CitERF13 in N. tabacum leaves, resulted in a significant decrease in net photosynthetic rate. Closer examination of photosynthetic activity of PSII and PSI indicated that CitERF13 overexpression led to declines of Fv/Fm, Y(II) and Y(I)...
March 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28166055/a-comprehensive-and-efficient-daily-quality-assurance-for-pbs-proton-therapy
#16
O Actis, D Meer, S König, D C Weber, A Mayor
There are several general recommendations for quality assurance (QA) measures, which have to be performed at proton therapy centres. However, almost each centre uses a different therapy system. In particular, there is no standard procedure for centres employing pencil beam scanning and each centre applies a specific QA program. Gantry 2 is an operating therapy system which was developed at PSI and relies on the most advanced technological innovations. We developed a comprehensive daily QA program in order to verify the main beam characteristics to assure the functionality of the therapy delivery system and the patient safety system...
March 7, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28163184/short-term-starvation-and-realimentation-helps-stave-off-edwardsiella-tarda-infection-in-red-sea-bream-pagrus-major
#17
Sipra Mohapatra, Tapas Chakraborty, Mohammad Ali Noman Reza, Sonoko Shimizu, Takahiro Matsubara, Kohei Ohta
Dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. Thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in Edwardsiella tarda infected red sea bream, we conducted a 20day experiment using 4 treatment groups, namely, pre-fed placebo (PFP); pre-starved placebo (PSP); pre-fed infected (PFI); and pre-starved infected (PSI), wherein a 5h E. tarda infection was done on the 11th day...
February 2, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28161893/identification-and-characterization-of-a-stable-intermediate-in-photosystem-i-assembly-in-tobacco
#18
Gal Wittenberg, Sari Järvi, Marta Hojka, Szilvia Z Tóth, Etienne H Meyer, Eva-Mari Aro, Mark Aurel Schöttler, Ralph Bock
PSI is the most efficient bioenergetic nanomachine in nature and one of the largest membrane protein complexes known. It is composed of 18 protein subunits that bind more than 200 co-factors and prosthetic groups. While the structure and function of PSI have been studied in great detail, very little is known about the PSI assembly process. In this work, we have characterized a PSI assembly intermediate in tobacco plants, which we named PSI*. We found PSI* to contain only a specific subset of the core subunits of PSI...
February 5, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28158282/the-effects-of-run-of-river-hydroelectric-power-schemes-on-invertebrate-community-composition-in-temperate-streams-and-rivers
#19
Gary S Bilotta, Niall G Burnside, Matthew D Turley, Jeremy C Gray, Harriet G Orr
Run-of-river (ROR) hydroelectric power (HEP) schemes are often presumed to be less ecologically damaging than large-scale storage HEP schemes. However, there is currently limited scientific evidence on their ecological impact. The aim of this article is to investigate the effects of ROR HEP schemes on communities of invertebrates in temperate streams and rivers, using a multi-site Before-After, Control-Impact (BACI) study design. The study makes use of routine environmental surveillance data collected as part of long-term national and international monitoring programmes at 22 systematically-selected ROR HEP schemes and 22 systematically-selected paired control sites...
2017: PloS One
https://www.readbyqxmd.com/read/28158177/correction-the-psi-prion-exists-as-a-dynamic-cloud-of-variants
#20
(no author information available yet)
[This corrects the article DOI: 10.1371/journal.pgen.1003257.].
February 2017: PLoS Genetics
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