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https://www.readbyqxmd.com/read/28530393/structural-factors-that-alter-the-redox-potential-of-quinones-in-cyanobacterial-and-plant-photosystem-i
#1
Keisuke Kawashima, Hiroshi Ishikita
Using the cyanobacterial and plant photosystem I (PSI) crystal structures and by considering the protonation states of all titratable residues, redox potentials (Em) of the two phylloquinones--A1A and A1B--were calculated. The calculated Em values were Em(A1A) = -773 mV and Em(A1B) = -818 mV for the plant PSI structure and Em(A1A) = -612 mV and Em(A1B) = -719 mV for the cyanobacterial PSI structure. Our analysis of the PSI crystal structures suggested that the side-chain orientations of Lys-B542 and Gln-B678 in the cyanobacterial crystal structure differ from these side-chain orientations in the plant crystal structure...
May 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28530191/green-synthesis-of-silver-nanoparticles-using-bovine-skin-gelatin-and-its-antibacterial-effect-on-clinical-bacterial-isolates
#2
Hosam I Salaheldin, Meshal H K Almalki, Gamal E H Osman
Nanoparticles are being increasingly used in day-to-day life. Therefore, concerns have been raised regarding their interactions with the surrounding environment. This study focused on a simple green method for synthesizing silver nanoparticles (Ag-NPs) in an autoclave at 15 psi (103 kPa) and 121°C. An aqueous solution of AgNO3 as a precursor of Ag-NPs and gelatin (type B) reducing and/or stabilizing (capping) agent were used. The effect of various AgNO3 concentrations of certain gelatin concentration and various gelatin concentrations at constant AgNO3 concentration, and autoclaving time, was studied...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28528550/isotachophoretic-fluorescence-in-situ-hybridization-of-intact-bacterial-cells
#3
Sui Ching Phung, Joan Marc Cabot, Mirek Macka, Shane M Powell, Rosanne M Guijt, Michael C Breadmore
A counter-pressure assisted capillary isotachophoresis method in combination with a sieving matrix and ionic spacer was used to perform in-line fluorescence in situ hybridization (FISH) of bacterial cells. A high concentration of sieving matrix (1.8% w/v HEC) was introduced at one end of the capillary and the bacterial cells were suspended in the spacer electrolyte for injection. Using a 2 min injection with 18 psi counter-pressure, 50% of the cells injected into the capillary were hybridized with the fluorescently labelled oligonucleotide and the excess unhybridized probe was separated from the hybridized cells-probe complexes in a two stage ITP method...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28526211/the-orbit-first-a-novel-surgical-treatment-protocol-for-secondary-orbitozygomatic-reconstruction
#4
Ruud Schreurs, Leander Dubois, Alfred G Becking, Thomas J J Maal
A novel surgical treatment sequence for secondary orbitozygomatic complex (OZC) reconstruction is described. Orbital reconstruction is performed before OZC repositioning. A surgical plan is made: the affected OZC is virtually osteotomized and aligned with a mirrored model of the unaffected OZC. A patient-specific implant (PSI) is designed for orbital reconstruction. Screw holes from the primary reconstruction are used for fixation. Primary screw hole positions at the repositioned OZC are embedded in the design, to guide OZC repositioning...
April 20, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28526061/photosynthesis-at-the-far-red-region-of-the-spectrum-in-acaryochloris-marina
#5
REVIEW
Syed Lal Badshah, Yahia Mabkhot, Salim S Al-Showiman
Acaryochloris marina is an oxygenic cyanobacterium that utilizes far-red light for photosynthesis. It has an expanded genome, which helps in its adaptability to the environment, where it can survive on low energy photons. Its major light absorbing pigment is chlorophyll d and it has α-carotene as a major carotenoid. Light harvesting antenna includes the external phycobilin binding proteins, which are hexameric rods made of phycocyanin and allophycocyanins, while the small integral membrane bound chlorophyll binding proteins are also present...
May 19, 2017: Biological Research
https://www.readbyqxmd.com/read/28525732/predictors-of-recovery-from-depression-and-anxiety-in-women-a-longitudinal-study-from-childbirth-to-6-years
#6
Radhika Shankar, Rinette Badker, Ursula Brain, Tim F Oberlander, Shaila Misri
OBJECTIVE: This study prospectively examined maternal biopsychosocial predictors of recovery from comorbid depression and anxiety from 25 weeks' gestation to 6 years postbirth. Specifically, the study investigated the influence of 1) maternal factors and 2) the child's behaviours and physical health on the course of the mother's depressed mood and anxiety. METHODS: Eighty-six women diagnosed with antenatal depression/anxiety were recruited through the Reproductive Mental Health Program and family practices in Vancouver...
May 2017: Canadian Journal of Psychiatry. Revue Canadienne de Psychiatrie
https://www.readbyqxmd.com/read/28524813/a-multifunctional-nanocomplex-for-enhanced-cell-uptake-endosomal-escape-and-improved-cancer-therapeutic-effect
#7
Patrick V Almeida, Mohammad-Ali Shahbazi, Alexandra Correia, Ermei Mäkilä, Marianna Kemell, Jarno Salonen, Jouni Hirvonen, Hélder A Santos
AIM: To evaluate the chemotherapeutic potential of a novel multifunctional nanocomposite encapsulating both porous silicon (PSi) and gold (Au) nanoparticles in a polymeric nanocomplex. MATERIALS & METHODS: The nanocomposite was physicochemically characterized and evaluated in vitro for biocompatibility, cellular internalization, endosomolytic properties, cytoplasmatic drug delivery and chemotherapeutic efficacy. RESULTS: The nanocomposites were successfully produced and exhibited adequate physicochemical properties and superior in vitro cyto- and hemocompatibilities...
May 19, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28522455/psa3-a-protein-on-the-stromal-face-of-the-thylakoid-membrane-promotes-photosystem-i-accumulation-in-cooperation-with-the-assembly-factor-pyg7
#8
Jie Shen, Rosalind Williams-Carrier, Alice Barkan
Photosystem I (PSI) is a large protein-pigment complex located in the thylakoid membrane in cyanobacteria, plants, and algae. Although the structure and components of PSI are well characterized, mechanisms that orchestrate its assembly are poorly understood. In this study, we discovered a novel nucleus-encoded protein, Photosystem I Assembly 3 (PSA3), that is required for PSI accumulation. PSA3 is conserved among green photosynthetic eukaryotes but is lacking in cyanobacteria. Mutations in the psa3 gene cause the specific loss of PSI in Arabidopsis and maize...
May 18, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28515738/chloroplast-atp-synthase-modulation-of-the-thylakoid-proton-motive-force-implications-for-photosystem-i-and-photosystem-ii-photoprotection
#9
Atsuko Kanazawa, Elisabeth Ostendorf, Kaori Kohzuma, Donghee Hoh, Deserah D Strand, Mio Sato-Cruz, Linda Savage, Jeffrey A Cruz, Nicholas Fisher, John E Froehlich, David M Kramer
In wild type plants, decreasing CO2 lowers the activity of the chloroplast ATP synthase, slowing proton efflux from the thylakoid lumen resulting in buildup of thylakoid proton motive force (pmf). The resulting acidification of the lumen regulates both light harvesting, via the qE mechanism, and photosynthetic electron transfer through the cytochrome b6f complex. Here, we show that the cfq mutant of Arabidopsis, harboring single point mutation in its γ-subunit of the chloroplast ATP synthase, increases the specific activity of the ATP synthase and disables its down-regulation under low CO2...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28512715/management-of-rainwater-harvesting-and-its-impact-on-the-health-of-people-in-the-middle-east-case-study-from-yatta-town-palestine
#10
Ilke Celik, Lina M A Tamimi, Issam A Al-Khatib, Defne S Apul
Water-related diseases are a primary problem in Palestine where many residents revert to harvested rainwater as their primary water source due to water shortages within the area. From an environmental engineering perspective, it is already well known that certain situations (e.g., cross contamination) reduce drinking water quality and ultimately cause diseases in a population. In this study, we investigated the social practices and situations that may lead to lower disease occurrence. Towards this goal, we surveyed 382 residents in Yatta to collect data on the water-related diseases that they experienced and the specific situations that might affect the disease occurrences such as the residents' practices (i) for maintaining a high quality of cistern water, (ii) for maintaining the environment around the cistern, and (iii) for managing the wastewater...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28512312/two-step-phase-shifting-differential-recording-digital-holographic-microscopy
#11
Jun-He Han, Ruo-Ping Li, Jun-Hui Liu, Fu-Sheng Hai, Ming-Ju Huang
We present two-step phase-shifting differential-recording digital holographic microscopy (TPD-DH in microscopy) for phase imaging of microscopic transparent elements. Two CCDs are employed to record two interferograms at two different defocusing distances. The interferograms on the two CCD cameras are shifted for a phase retarder 0 and π via an all-optics phase shifting unit. A novel algorithm is proposed to reconstruct both amplitude and phase distributions of the object wave from the recorded interferograms...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28511946/national-incidence-of-patient-safety-indicators-in-the-total-hip-arthroplasty-population
#12
Joseph E Tanenbaum, Derrick M Knapik, Glenn D Wera, Steven J Fitzgerald
BACKGROUND: The Centers for Medicare & Medicaid Services use the incidence of patient safety indicators (PSIs) to determine health care value and hospital reimbursement. The national incidence of PSI has not been quantified in the total hip arthroplasty (THA) population, and it is unknown if patient insurance status is associated with PSI incidence after THA. METHODS: All patients in the Nationwide Inpatient Sample (NIS) who underwent THA in 2013 were identified using the International Classification of Diseases, Ninth Revision, Clinical Modification codes...
April 12, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28507783/periprosthetic-shoulder-infection-an-overview
#13
REVIEW
Nicolas Bonnevialle, Florence Dauzères, Julien Toulemonde, Fanny Elia, Jean-Michel Laffosse, Pierre Mansat
Periprosthetic shoulder infection (PSI) is rare but potentially devastating. The rate of PSI is increased in cases of revision procedures, reverse shoulder implants and co-morbidities. One specific type of PSI is the occurrence of low-grade infections caused by non-suppurative bacteria such as Propionibacterium acnes or Staphylococcus epidemermidis.Success of treatment depends on micro-organism identification, appropriate surgical procedures and antibiotic administration efficiency. Post-operative early PSI can be treated with simple debridement, while chronic PSI requires a one- or two-stage revision procedure...
April 2017: EFORT Open Rev
https://www.readbyqxmd.com/read/28500496/patient-specific-instrumentation-improves-alignment-of-lateral-unicompartmental-knee-replacements-by-novice-surgeons
#14
Chin Ting Justin Ng, Simon Newman, Simon Harris, Susannah Clarke, Justin Cobb
PURPOSE: Patient-specific instrumentation (PSI) has the potential to offer numerous benefits-not least of all, improved resection accuracy; but its potential has not been realised in clinical studies. An explanation may be the focus of such studies on the total knee replacement (TKR-a common procedure, with which surgeons are generally very familiar. Consequently, we sought to investigate the potential role of PSI in guiding novice surgeons to perform the more technically demanding and less familiar lateral unicondylar knee replacement (LUKR)...
May 13, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28498174/adult-women-s-experiences-of-urinary-incontinence-a-systematic-review-of-qualitative-evidence
#15
Adilson Mendes, Luiza Hoga, Bruna Gonçalves, Pâmela Silva, Priscilla Pereira
BACKGROUND: Women are affected dramatically by urinary incontinence (UI). This disease is currently considered as epidemic. OBJECTIVE: The objective of this review is to identify, through the best available evidence, how women experience UI worldwide. INCLUSION CRITERIA TYPES OF PARTICIPANTS: The current review included studies of adult women who had experienced UI. PHENOMENA OF INTEREST: Women with UI from various social and cultural settings were included in this review...
May 2017: JBI Database of Systematic Reviews and Implementation Reports
https://www.readbyqxmd.com/read/28497476/optimization-of-a-novel-large-field-of-view-distortion-phantom-for-mr-only-treatment-planning
#16
Ryan G Price, Robert A Knight, Ken-Pin Hwang, Ersin Bayram, Siamak P Nejad-Davarani, Carri K Glide-Hurst
PURPOSE: MR-only treatment planning requires images of high geometric fidelity, particularly for large fields of view (FOV). However, the availability of large FOV distortion phantoms with analysis software is currently limited. This work sought to optimize a modular distortion phantom to accommodate multiple bore configurations and implement distortion characterization in a widely implementable solution. METHOD AND MATERIALS: To determine candidate materials, 1...
May 12, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28492483/pcvmzm-using-the-probabilistic-classification-vector-machines-model-combined-with-a-zernike-moments-descriptor-to-predict-protein-protein-interactions-from-protein-sequences
#17
Yanbin Wang, Zhuhong You, Xiao Li, Xing Chen, Tonghai Jiang, Jingting Zhang
Protein-protein interactions (PPIs) are essential for most living organisms' process. Thus, detecting PPIs is extremely important to understand the molecular mechanisms of biological systems. Although many PPIs data have been generated by high-throughput technologies for a variety of organisms, the whole interatom is still far from complete. In addition, the high-throughput technologies for detecting PPIs has some unavoidable defects, including time consumption, high cost, and high error rate. In recent years, with the development of machine learning, computational methods have been broadly used to predict PPIs, and can achieve good prediction rate...
May 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28492349/a-novel-compressive-stress-based-osteoarthritis-like-chondrocyte-system
#18
In-Chi Young, Sung-Ting Chuang, Amit Gefen, Wei-Ting Kuo, Chun-Ting Yang, Chia-Hsien Hsu, Feng-Huei Lin
Mechanical stress damage and insufficient self-repair can contribute to osteoarthritis (OA) in the affected joint. As the effects of stress on chondrocyte metabolism can regulate cartilage homeostasis, the specific stress-response condition is therefore a key to the generation of an OA disease model. We aimed to produce a specific stress- and cell-based OA model after evaluating the metabolic responses of chondrocytes in response to a series of static and cyclic compression stressors. A static load exceeding 40 psi initiated extracellular matrix (ECM) degradation through a decrease in the sulphated-glycosaminoglycan (GAG) content, upregulation of catabolic matrix metalloproteinase (MMP)-13 encoding gene expression, and downregulation of the ECM-related aggrecan and type II collagen encoding genes within 24 h...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28487478/flavodiiron-proteins-promote-fast-and-transient-o2-photoreduction-in-chlamydomonas
#19
Frederic Chaux, Adrien Burlacot, Malika Mekhalfi, Pascaline Auroy, Stéphanie Blangy, Pierre Richaud, Gilles Peltier
During oxygenic photosynthesis, the reducing power generated by light energy conversion is mainly used to reduce carbon dioxide. In bacteria and archae, flavodiiron (Flv) proteins catalyze O2 or NO reduction, thus protecting cells against oxidative or nitrosative stress. These proteins are found in cyanobacteria, mosses and microalgae, but have been lost in angiosperms. Here, we used chlorophyll fluorescence and oxygen exchange measurement using [18O]-labeled O2 and a Membrane Inlet Mass Spectrometer (MIMS) to characterize Chlamydomonas reinhardtii flvB insertion mutants devoid of both FlvB and FlvA proteins...
May 9, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28486575/burst-suppression-ratio-underestimates-absolute-duration-of-electroencephalogram-suppression-compared-with-visual-analysis-of-intraoperative-electroencephalogram
#20
W G Muhlhofer, R Zak, T Kamal, B Rizvi, L P Sands, M Yuan, X Zhang, J M Leung
Background: Machine-generated indices based on quantitative electroencephalography (EEG), such as the patient state index (PSI™) and burst-suppression ratio (BSR), are increasingly being used to monitor intraoperative depth of anaesthesia in the endeavour to improve postoperative neurological outcomes, such as postoperative delirium (POD). However, the accuracy of the BSR compared with direct visualization of the EEG trace with regard to the prediction of POD has not been evaluated previously...
May 1, 2017: British Journal of Anaesthesia
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