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Seul Gi Lee, Jinhye Kim, Hyunjoon Park, Wilhelm Holzapfel, Kwang-Won Lee
No abstract text is available yet for this article.
December 5, 2017: Food and Chemical Toxicology
María Alejandra Vorphal, Carola Bruna, Traudy Wandersleben, Jorge Dagnino-Leone, Francisco Lobos-González, Elena Uribe, José Martínez-Oyanedel, Marta Bunster
BACKGROUD: Ferredoxin NADP(H) oxidoreductases (EC (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd...
December 8, 2017: Biological Research
Ángel Ferrero-Serrano, Zhao Su, Sarah M Assmann
We studied physiological mechanisms of photoavoidance and photoprotection of a dwarf rice mutant with erect leaves, d1, in which the RGA1 gene, which encodes the Gα subunit of the heterotrimeric G protein, is non-functional. d1 leaves exhibit lower leaf temperature and higher Photochemical Reflectance Index (PRI) relative to wild-type (WT), indicative of increased photoavoidance and more efficient light harvesting. RNA-seq analysis of flag leaves revealed that mRNA levels of genes encoding HSPs, enzymes associated with chlorophyll breakdown, and ROS scavengers were down-regulated in d1...
December 7, 2017: Plant, Cell & Environment
Maxwell T Robinson, David E Cliffel, G Kane Jennings
The photosynthetic protein, Photosystem I (PSI), has been used as a photoactive species within a host of biohybrid photoelectrochemical systems. PSI multilayer films at electrode surfaces provide greatly improved solar energy conversion relative to homologous monolayer films. While the photocatalytic effect of PSI multilayers has been theorized as an electrolyte-mediated mechanism, no comprehensive, first-principles modeling study has been presented. In this work, we develop and optimize an electrochemical reaction-diffusion model to replicate the significant electrochemical, physicochemical, and transport processes that underpin photocurrent development of a PSI multilayer film...
December 6, 2017: Journal of Physical Chemistry. B
Karan Kapoor, Derek J Cashman, Luke Nientimp, Barry D Bruce, Jerome Yves Baudry
The stromal domain (PsaC, D, and E) of photosystem I (PSI) in Cyanobacteria accepts electrons from PsaA and PsaB of photosystem I (PSI). These electrons then reduce transiently bound ferredoxin (Fd) or flavodoxin. Experimental structures exist for all of these protein partners individually, yet to date, no experimental structure of the PSI/Fd complexes is presently available. Molecular models of Fd docked into the stromal domain of the cyanobacterial PSI site are constructed here starting from X-ray and NMR structures of PSI and Fd...
December 6, 2017: Journal of Physical Chemistry. B
Gaurav Das, Shyamtanu Chattoraj, Somen Nandi, Prasenjit Mondal, Abhijit Saha, Kankan Bhattacharyya, Surajit Ghosh
The fluorescence dynamics of Photosystem I (PSI) in bulk water and inside a confined environment like a liposome have been investigated using time resolved confocal microscopy. In bulk water, PSI exhibits a major emission peak at ∼680 nm, while in the liposome it exhibits a markedly blue shifted emission maximum at ∼485 nm. This is indicative of conformational changes due to entrapment and emergence of a stressed conformation of PSI inside the liposome. The observed time constants for the fluorescence lifetime of PSI inside the liposome are significantly high as opposed to PSI in bulk water...
December 6, 2017: Physical Chemistry Chemical Physics: PCCP
Muralikrishna Narra, Raghu Ellendula, Srinivas Kota, Bharathkumar Kalva, Yashodhara Velivela, Sadanandam Abbagani
Here, we report the optimized conditions for biolistic particle delivery-mediated genetic transformation of bitter melon using petiole segments. In this study, DNA-coated gold particles of 0.6 µm were used for optimizing the parameters of transformation and eventually regeneration of bitter melon putative transgenics. Initially, biolistic parameters namely helium pressure and macrocarrier to target tissue distance, were optimized using binary vector pBI121 carrying both β-glucuronidase gene (GUS) and neomycin phosphotransferase II gene (npt II) as a reporter and as a selectable marker gene, respectively...
January 2018: 3 Biotech
Zahra Zamanian, Zahra Sedaghat, Masoud Hemehrezaee, Farahnaz Khajehnasiri
Background: Thermal component of the atmospheric environment is an important issue which is related to human's health. Thermal environment includes both heat exchange conditions (stress) and the physiological response (strain). The aim of this study was to measure the association of heat indices (PSI, HSI, Humidex) especially subjective one (STI) with some physiological parameters (Blood pressure, pulse rate and skin temperature). Methods: This is a cross-sectional study conducted on 387 male farmers on Boukan, West Azerbaijan, Iran in 2016...
2017: Journal of Environmental Health Science & Engineering
Yongli Wang, Qin Wan, Zhaoming Huang, Li Huang, Feng Kong
The Multi-dimensional Scale of Perceived Social Support (MSPSS) is one of the most extensively used instruments to assess social support. The purpose of this research was to test the reliability, factorial validity, concurrent validity and measurement invariance across gender groups of the MSPSS in Chinese parents of children with cerebral palsy. A total of 487 participants aged 21-55 years were recruited to complete the Chinese MSPSS and Parenting Stress Index-Short Form (PSI-SF). Composite reliability was calculated as the internal consistency of the Chinese MSPSS and a (multi-group) confirmatory factor analysis (CFA) was conducted to test the factorial validity and measurement invariance across gender...
2017: Frontiers in Psychology
Guoxiang Wang, Yi Ping Zhang, Zhongwen Gao, Lisa B E Shields, Fang Li, Tianci Chu, Huayi Lv, Thomas Moriarty, Xiao-Ming Xu, Xiaoyu Yang, Christopher B Shields, Jun Cai
Abusive head trauma (AHT) is the leading cause of death from trauma in infants and young children. An AHT animal model was developed on 12-day-old mice subjected to 90° head extension-flexion sagittal shaking repeated 30, 60, 80, and 100 times. The mortality and time until return of consciousness were dependent on the number of repeats and severity of the injury. Under 60 times of repeated head shakings, the pups demonstrated apnea and/or bradycardia immediately after injury. Acute oxygen desaturation was observed by pulse oximetry during respiratory and cardiac suppression...
November 30, 2017: Disease Models & Mechanisms
Xiaohuan Mu, Qinwu Chen, Fanjun Chen, Lixing Yuan, Guohua Mi
Nitrogen is a major limiting factor for crop productivity. The relationship between photosynthesis and nitrogen nutrition has been widely studied. However, the molecular response of leaf photosynthesis to low nitrogen supply in crops is less clear. In this study, RNA sequencing technology (RNA-Seq) was used to investigate the gene expressions related to photosynthesis in maize in response to low nitrogen supply. It was found that low nitrogen supply down-regulated the expression of genes involved in photosystem I (PSI) and photosystem II (PSII)...
December 5, 2017: International Journal of Molecular Sciences
Yoshinobu Kato, Kazuhiko Sugimoto, Toshiharu Shikanai
The chloroplast NADH dehydrogenase-like (NDH) complex is structurally similar to respiratory complex I and mediates photosystem I (PSI) cyclic electron flow. In Arabidopsis, chloroplast NDH is composed of at least 29 subunits and associates with two copies of PSI to form the NDH-PSI supercomplex. Here, we found that CHLORORESPIRATORY REDUCTION 3 (CRR3) is an assembly factor required for the accumulation of subcomplex B (SubB) of chloroplast NDH. In sucrose density gradient centrifugation, CRR3 was detected in three protein complexes...
December 4, 2017: Plant Physiology
Abbas Abbasnia, Mahmood Alimohammadi, Amir Hossein Mahvi, Ramin Nabizadeh, Mahmood Yousefi, Ali Akbar Mohammadi, Hassan Pasalari, Majid Mirzabeigi
The aims of this study were to assess and analysis of drinking water quality of Chabahar villages in Sistan and Baluchistan province by water quality index (WQI) and to investigate the water stability in subjected area. The results illustrated that the average values of LSI, RSI, PSI, LS, and AI was 0.5 (±0.34), 6.76 (±0.6), 6.50 (±0.99), 2.71 (±1.59), and 12.63 (±0.34), respectively. The calculation of WQI for groundwater samples indicated that 25% of the samples could be considered as excellent water, 50% of the samples were classified as good water category and 25% of the samples showed poor water category...
February 2018: Data in Brief
Malin Assarsson, Albert Duvetorp, Olaf Dienus, Jan Söderman, Oliver Seifert
Changes in the skin microbiome have been shown to promote cutaneous inflammation. The skin microbiome of patients with chronic plaque type psoriasis was analysed before and after treatment with narrowband ultraviolet B (UVB). Swab samples of the microbiome were taken from lesional and non-lesional skin of 26 patients. Microbiotas were characterized by sequencing 16S rRNA bacterial genes on the Illumina MiSeq platform. Lesional skin microbiome diversity correlated with psoriasis severity (measured with the Psoriasis Area and Severity Index; PASI)...
December 4, 2017: Acta Dermato-venereologica
Maksymilian Zienkiewicz, Tomasz Krupnik, Anna Drożak, Wioleta Wasilewska, Anna Golke, Elżbieta Romanowska
We have successfully produced single-cell colonies of C. merolae mutants, lacking the PsbQ' subunit in its PSII complex by application of DTA-aided mutant selection. We have investigated the physiological changes in PSII function and structure and proposed a tentative explanation of the function of PsbQ' subunit in the PSII complex. We have improved the selectivity of the Cyanidioschyzon merolae nuclear transformation method by the introduction of diphtheria toxin genes into the transformation vector as an auxiliary selectable marker...
December 1, 2017: Plant Molecular Biology
Christina Jerosch-Herold, Rachel Chester, Lee Shepstone, Joshua I Vincent, Joy C MacDermid
PURPOSE: The shoulder pain and disability index (SPADI) has been extensively evaluated for its psychometric properties using classical test theory (CTT). The purpose of this study was to evaluate its structural validity using Rasch model analysis. METHODS: Responses to the SPADI from 1030 patients referred for physiotherapy with shoulder pain and enrolled in a prospective cohort study were available for Rasch model analysis. Overall fit, individual person and item fit, response format, dependence, unidimensionality, targeting, reliability and differential item functioning (DIF) were examined...
November 29, 2017: Quality of Life Research
Tara N Furstenau, Reed A Cartwright
Inbreeding in hermaphroditic plants can occur through two different mechanisms: biparental inbreeding, when a plant mates with a related individual, or self-fertilization, when a plant mates with itself. To avoid inbreeding, many hermaphroditic plants have evolved self-incompatibility (SI) systems which prevent or limit self-fertilization. One particular SI system-homomorphic SI-can also reduce biparental inbreeding. Homomorphic SI is found in many angiosperm species, and it is often assumed that the additional benefit of reduced biparental inbreeding may be a factor in the success of this SI system...
2017: PeerJ
Patrycja Haniewicz, Mateusz Abram, Lukas Nosek, Joanna Kirkpatrick, Eithar El-Mohsnawy, Julian D Janna Olmos, Roman Kouril, Joanna M Kargul
The monomeric PSI-LHCI supercomplex from an extremophilic red alga Cyanidioschyzon merolae represents an intermediate evolutionary link between the cyanobacterial PSI reaction centre and its green algal/higher plant counterpart. We show that the C. merolae PSI-LHCI supercomplex is characterized by robustness in various extreme conditions. By combination of biochemical, spectroscopic, mass spectrometry and electron microscopy/single particle analyses we dissected three molecular mechanisms underlying inherent robustness of C...
November 29, 2017: Plant Physiology
T M Pinto, C Samorinha, I Tendais, S Silva, B Figueiredo
STUDY QUESTION: Do mode of conception [ART versus Natural (NC)] and depression have an interactive effect on antenatal paternal adjustment and paternal attitudes? SUMMARY ANSWER: Depression increased the negative effect of ART on antenatal paternal adjustment and paternal attitudes, specifically on antenatal marital relationship satisfaction. WHAT IS KNOWN ALREADY: Research on antenatal paternal adjustment and paternal attitudes after ART is scarce and has produced inconsistent results...
November 24, 2017: Human Reproduction
Xiaoqiong Qin, Tianliang Yang, Mengshi Yang, Lu Zhang, Mingsheng Liao
Since the Persistent Scatterer Synthetic Aperture Radar (SAR) Interferometry (PSI) technology allows the detection of ground subsidence with millimeter accuracy, it is becoming one of the most powerful and economical means for health diagnosis of major transportation infrastructures. However, structures of different types may suffer from various levels of localized subsidence due to the different structural characteristics and subsidence mechanisms. Moreover, in the complex urban scenery, some segments of these infrastructures may be sheltered by surrounding buildings in SAR images, obscuring the desirable signals...
November 29, 2017: Sensors
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