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Methylene blue

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https://www.readbyqxmd.com/read/28816073/overactive-bladder-treatment-application-of-methylene-blue-to-improve-the-injection-technique-of-onabotulinum-toxin-a
#1
Michał Szczypior, Wojciech Połom, Marcin Markuszewski, Krzesimir Ciura, Magdalena Buszewska-Forajta, Julia Jacyna, Michał Markuszewski, Marcin Matuszewski
OBJECTIVE: The aim of this study was to test the addition of methylene blue (MB) to onabotulinum toxin A (BTX-A) solution in overactive bladder (OAB) treatment, as a means of facilitating observation of the injection site and assessing the distribution of the drug under the bladder mucosa during injection. Pharmacological interactions between BTX-A and MB were also evaluated. MATERIALS AND METHODS: The study was conducted between December 2014 and April 2016 on 30 patients: six males and 24 females (median age 57...
August 17, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/28815483/photocatalytic-properties-of-co3o4-coated-tio2-powders-prepared-by-plasma-enhanced-atomic-layer-deposition
#2
Xi-Rui Zhao, Yan-Qiang Cao, Jun Chen, Lin Zhu, Xu Qian, Ai-Dong Li, Di Wu
Co3O4-coated commercial TiO2 powders (P25) p-n junction photocatalysts were prepared by plasma-enhanced atomic layer deposition (PEALD) technique. The structure, morphology, bandgap, and photocatalytic properties under ultraviolet light were investigated systematically. Although the deposition of Co3O4 does not change the anatase structure and crystallite size of P25 powders, the ultraviolet photocatalytic activity has been improved evidently. For the Co3O4-coated P25 powders, the trace Co ions exist as Co3O4 nanoparticles attached to TiO2 powder surface instead of the occupation of Ti(4+) position in TiO2 lattice...
August 16, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28813714/effects-of-riboflavin-and-ultraviolet-illumination-on-the-biomechanical-properties-of-conjunctiva
#3
Harrison Man Hin Chan, Bonnie Nga Kwan Choy, Jimmy Shiu Ming Lai
PURPOSE: Our experiment evaluated the biomechanical effects of riboflavin and ultraviolet A illumination, in terms of covalent bonding, permeability, and indentation rupture force, on excised cattle conjunctiva. MATERIALS AND METHODS: Cattle conjunctiva was extracted and divided into the treatment group, which was soaked in riboflavin, followed by ultraviolet light illumination, and the control group. Samples were subsequently analysed on a Fourier transform infrared spectrum test to identify the presence of amide bonds, a permeability test to assess the diffusion of methylene blue across conjunctiva, and an indentation rupture force test...
August 17, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28813710/is-endoscopic-vasectomy-just-a-dream-an-ex-vivo-study-on-feasibility-and-reliability-of-endoluminal-occlusion-of-porcine-vas-deferens
#4
Daniel Schlager, Moritz Maas, Simon Hein, Fabian Adams, Martin Schoenthaler, Bjoern Spittau, Ulrich Wetterauer, Arkadiusz Miernik
INTRODUCTION AND AIM: Surgical vasectomy remains the gold standard for fertility control in men. Endoluminal occlusion of the seminal ducts, thus avoiding an external incision, may become an appealing alternative to this approach. As our group has shown, nontraumatic endoscopic inspection of the seminal ducts is feasible in the human cadaver. We investigated the feasibility and reliability of occlusion using several commercially available medical sealing agents in the porcine vas deferens (VD)...
August 17, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28813104/poly-methylene-blue-modified-electrode-for-indirect-electrochemical-sensing-of-oh-radicals-and-radical-scavengers
#5
Walter A Braun, Bruna C Horn, Lucélia Hoehne, Simone Stülp, Marcelo B DA Rosa, Maurício Hilgemann
A new modified electrode for indirect sensing of OH· and radical scavengers was described. The electrochemical polymerization of methylene blue in aqueous solutions and the properties of the resulting films on a glassy carbon electrode were carried out using cyclic voltammetry. A surface coverage of 1.11 × 109mol cm2 was obtained, revealing a complete surface coverage of the polymeric film on the electrode surface. OH· was able to destroy the poly(methylene blue) film by exposure to a Fenton solution. The loss of the electrochemical signal of the residual polymeric film attached to the electrode surface was related to the extent of its dissolution...
August 14, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28811108/methylene-blue-guided-debridement-as-an-intraoperative-adjunct-for-the-surgical-treatment-of-periprosthetic-joint-infection
#6
Jeremy D Shaw, Steve Miller, Anna Plourde, Daniel L Shaw, Rosanna Wustrack, Erik N Hansen
BACKGROUND: Current methods to identify infected tissue in periprosthetic joint infection (PJI) are inadequate. The purpose of this study was (1) to assess methylene blue-guided surgical debridement as a novel technique in PJI using quantitative microbiology and (2) to evaluate clinical success based on eradication of infection and infection-free survival. METHODS: Sixteen total knee arthroplasty patients meeting Musculoskeletal Infection Society criteria for PJI undergoing the first stage of 2-stage exchange arthroplasty were included in this prospective study...
July 21, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28810234/electrochemical-aptamer-based-nanosensor-fabricated-on-single-au-nanowire-electrodes-for-adenosine-triphosphate-assay
#7
Dongmei Wang, Xiaoqing Xiao, Shen Xu, Yong Liu, Yongxin Li
In this work, single Au nanowire electrodes (AuNWEs) were fabricated by laser-assisted pulling/hydrofluoric acid (HF) etching process, which then were characterized by transmission electron microscopy (TEM), electrochemical method and finite-element simulation. The as-prepared single AuNWEs were used to construct electrochemical aptamer-based nanosensors (E-AB nanosensors) based on the formation of Au-S bond that duplex DNA tagged with methylene blue (MB) was modified on the surface of electrode. In the presence of adenosine triphosphate (ATP), the MB-labeled aptamer dissociated from the duplex DNA due to the strong specific affinity between aptamer and target, which lead to the reduction of MB electrochemical signals...
August 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28809411/a-monomeric-bowl-like-pyrogallol-4-arene-ti12-coordination-complex
#8
Kongzhao Su, Mingyan Wu, Yanxi Tan, Wenjing Wang, Daqiang Yuan, Maochun Hong
Herein, an unprecedented monomeric bowl-like coordination complex, Ti12PgC3 (PgC3 = C-propylpyrogallol[4]arene), has been successfully synthesized. To the best of our knowledge, Ti12PgC3 not only presents the first pyrogallol[4]arene-based titanium coordination complex, but also the highest nuclearity titanium coordination complex in the metal-calixarene system. In addition, this titanium coordination complex can effectively degrade the methylene blue (MB) dye under sunlight.
August 15, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28806655/magnetic-reduced-graphene-oxide-loaded-hydrogels-highly-versatile-and-efficient-adsorbents-for-dyes-and-selective-cr-vi-ions-removal
#9
Fatima Halouane, Yavuz Oz, Dalila Meziane, Alexandre Barras, Jean Juraszek, Santosh K Singh, Sreekumar Kurungot, Pankaj K Shaw, Rana Sanyal, Rabah Boukherroub, Amitav Sanyal, Sabine Szunerits
The formation of composites of reduced graphene oxide (rGO) and magnetic nanoparticles (MP) has flourished in recent years as they combine the advantages of both nanomaterials. Most of these composite materials are prepared by in situ formation of MP onto rGO or by the post-adsorption onto rGO. We report here on a simple and highly controlled method for the fabrication of different magnetic 3D rGO-loaded hydrogels. Cellulose bound magnetic nanoparticles (MP@cellulose) were synthesized by chemical co-precipitation and loaded together with rGO into poly(ethylene glycol) dimethacrylate based hydrogels during their fabrication using photo-polymerization...
July 28, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28805161/eco-friendly-and-biocompatible-crosslinked-carboxymethylcellulose-hydrogels-as-adsorbents-for-the-removal-of-organic-dye-pollutants-for-environmental-applications
#10
Nádia S V Capanema, Alexandra A P Mansur, Herman S Mansur, Anderson C de Jesus, Sandhra M Carvalho, Poliane Chagas, Luiz C de Oliveira
In this study new eco-friendly hydrogel adsorbents were synthesized based on carboxymethylcellulose (CMC, degree of substitution DS = 0.7) chemically crosslinked with citric acid (CA) using a green process in aqueous solution and applied for the adsorption of methylene blue (MB). Spectroscopic analyses demonstrated the mechanism of crosslinking through the reaction of hydroxyl functional groups from CMC with CA. These CMC hydrogels showed very distinct morphological features dependent on the extension of crosslinking and their nanomechanical properties were drastically increased by approximately 300% after crosslinking with 20% CA (e...
August 13, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28805061/a-triple-helix-molecular-switch-electrochemical-ratiometric-biosensor-for-ultrasensitive-detection-of-nucleic-acids
#11
Erhu Xiong, Zhenzhen Li, Xiaohua Zhang, Jiawan Zhou, Xiaoxia Yan, Yunqing Liu, Jinhua Chen
Biomolecular receptors such as nucleic acids that switch between two or more conformations upon binding to a specific target can be used to build specific and sensitive biosensors. In this work, based on the electrochemical dual-signaling ratiometric strategy and triple-helix molecular switch, we developed a selective, reusable, and simple electrochemical DNA (E-DNA) biosensor for target DNA (T-DNA) detection. A hairpin DNA capture probe labeled with methylene blue (MB-DNA) self-assembles on the surface of gold electrode (GE) through Au-S bond, and then a single-strand DNA modified with two ferrocenes (Fc-DNA) on each end to enhance the oxidation signal hybridizes with the MB-DNA to form a triple-helix conformation...
August 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28803519/a-case-report-of-benzocaine-induced-methemoglobinemia
#12
Chaundra R Sewell, Michael P Rivey
Methemoglobinemia is a serious medical condition characterized by the disrupted binding of oxygen to iron on hemoglobin, with a consequent impaired oxygen delivery to body tissues. Various drugs including the local anesthetics such as benzocaine can cause acquired methemoglobinemia. The reported case describes methemoglobinemia that occurred in association with the use of topical benzocaine spray and lozenges in a previously healthy 51-year-old female who had undergone colon surgery to remove a bleeding polyp...
January 1, 2017: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/28803003/pathogenic-features-and-characteristics-of-food-borne-pathogens-biofilm-biomass-viability-and-matrix
#13
REVIEW
Shiqi Lin, Ling Yang, Chen Gu, Bing Li, Dingqiang Chen, Lin Li, Zhenbo Xu
Biofilm is a ubiquitous growth pattern of bacterial species survival but is notorious for its threat on public health and food contamination. Extensive studies of the biofilm structure, formation, quantification, quorum sensing system and underlying control strategies have been reported during the past decades. Insightful elucidation of the pathogenic features and characteristic of bacterial biofilm can facilitate in devising appropriate control strategies for biofilm eradication. Therefore, this review mainly summarized the pathogenic features of biofilms from food borne microorganisms, including the biomass (which could be quantified using crystal violet and fluorogenic dye Syto9 assays), viability (which could be determined by tetrazolium salts, fluorescein diacetate, resazurin staining and alamar blue assays) and matrix (which are commonly detected by dimethyl methylene blue and wheat germ agglutinin assays)...
August 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28802354/a-novel-management-of-streptococcal-pharyngotonsillar-infections-by-laser-activated-silver-nanoparticles-and-methylene-blue-conjugate-in%C3%A2-vitro-study
#14
Ahmed Kassab, Ola Dabous, Mona Morsy
INTRODUCTION: Species of Streptococcus are classified based on their hemolytic properties into alpha and beta types. And, since antimicrobial drug resistance is an increasingly problematic issue, the efforts to develop modalities that would overcome this obstacle and avoid antibiotic side effects is an ongoing challenge. METHODS AND MATERIALS: 20 patients from both sexes were selected. The isolated organisms were identified according to standard laboratory methods...
September 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28801677/polyacrylamide-phytic-acid-polydopamine-conducting-porous-hydrogel-for-efficient-removal-of-water-soluble-dyes
#15
Zhen Zhao, Leijiao Li, Girma Selale Geleta, Lina Ma, Zhenxin Wang
Removal of toxic dyes from wastewater has become a hot topic in both academic and industrial fields since there is growing concern about the threat of sewage to human health. Herein, we demonstrate that the three-dimensional porous polyacrylamide-phytic acid-polydopamine (termed as PAAM/PA/PDA) hydrogel can be served as reusable adsorbent with high efficiency for either anionic or cationic dyes. Using methyl blue (MB), methylene blue (YMB), methyl violet (MV) and neutral red (NR) as model dyes, we investigate the effect of pH, temperature, dye concentration, and PAAM/PA/PDA hydrogel mass on the adsorption...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800557/predicting-and-experimental-evaluating-bio-electrochemical-synthesis-a-case-study-with-clostridium-kluyveri
#16
Christin Koch, Anne Kuchenbuch, Frauke Kracke, Paul V Bernhardt, Jens Krömer, Falk Harnisch
Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET. Among others, Clostridia are believed to represent electroactive microbes being highly promising for microbial electrosynthesis...
July 25, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28800432/linear-and-circular-dichroism-characterization-of-thionine-binding-mode-with-dna-polynucleotides
#17
Eimer Mary Tuite, Bengt Nordén
The binding mode of thionine (3,7-diamino-5-phenothiazinium) with alternating and non-alternating DNA polynucleotides at low binding ratios was conclusively determined using linear and circular dichroism spectroscopies. The binding to [poly(dG-dC)]2 and poly(dG)·poly(dC) was purely intercalative and was insensitive to ionic strength. Intercalative binding to [poly(dA-dT)]2 is observed at low ionic strength, but a shift of some dye to an non-intercalative mode is observed as the background salt concentration increases...
August 1, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28800215/c-fibers-wse2-nanoplates-core-shell-composite-highly-efficient-solar-driven-photocatalyst
#18
Hong Li, Zhijian Peng, Jingwen Qian, Meng Wang, Chengbiao Wang, Xiuli Fu
Recently, WSe2 as a typical transition metal dichalcogenide compound has attracted extensive attention due to its potential applications in electronic and optoelectronic devices. However, WSe2 alone cannot be directly used as photocatalyst, due to its inferior performance possibly caused by the strong recombination of photogenerated electron-hole pairs. Here a novel C fibers@WSe2 nanoplates core-shell composite (NPCSC) was successfully synthesized via facile, one-step thermal evaporation, in which numerous WSe2 thin nanoplates were in-situ, densely and even vertically grown on the surface of the C fibers...
August 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28799332/attaching-the-nora-efflux-pump-inhibitor-inf55-to-methylene-blue-enhances-antimicrobial-photodynamic-inactivation-of-methicillin-resistant-staphylococcus-aureus-in-vitro-and-in-vivo
#19
Ardeshir Rineh, Naveen K Dolla, Anthony R Ball, Maria Magana, John B Bremner, Michael R Hamblin, George P Tegos, Michael J Kelso
Antimicrobial photodynamic inactivation (aPDI) uses photosensitizers (PSs) and harmless visible light to generate reactive oxygen species (ROS) and kill microbes. Multidrug efflux systems can moderate the phototoxic effects of PSs by expelling the compounds from cells. We hypothesized that increasing intracellular concentrations of PSs by inhibiting efflux with a covalently attached efflux pump inhibitor (EPI) would enhance bacterial cell phototoxicity and reduce exposure of neighbouring host cells to damaging ROS...
August 11, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28799328/a-tunable-signal-off-and-signal-on-electrochemical-cisplatin-sensor
#20
Yao Wu, Rebecca Y Lai
We report the first electrochemical cisplatin sensor fabricated with a thiolated and methylene blue (MB)-modified oligo-adenine(A)-guanine(G) DNA probe. Depending on the probe coverage, the sensor can behave as a "signal-off" or "signal-on" sensor. For the high-coverage sensor, formation of intrastrand Pt(II)-AG adducts rigidifies the oligo-AG probe, resulting in a concentration-dependent decrease in the MB signal. For the low-coverage sensor, the increase in probe-to-probe spacing enables binding of cisplatin via the intrastrand GNG motif (N = A), generating a bend in the probe which results in an increase in the MB current...
August 11, 2017: Analytical Chemistry
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