keyword
MENU ▼
Read by QxMD icon Read
search

Cell volume

keyword
https://www.readbyqxmd.com/read/28918472/a-reliable-protocol-for-colorimetric-determination-of-iron-oxide-nanoparticle-uptake-by-cells
#1
Daiana K Deda, Roberta M Cardoso, Mayara K Uchiyama, Christiane Pavani, Sergio H Toma, Mauricio S Baptista, Koiti Araki
Size, shape, and surface properties of superparamagnetic iron oxide nanoparticles (SPIONs) can influence their interaction with biological systems, particularly the incorporation by tumor cells and consequently the biological activity and efficiency in biomedical applications. Several strategies have been used to evaluate cellular uptake of SPIONs. While qualitative methods are generally based on microscopy techniques, quantitative assays are carried out by techniques such as inductively coupled plasma-mass spectrometry and flow cytometry...
September 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28918285/controlling-cell-volume-for-efficient-phb-production-by-halomonas
#2
Xiao-Ran Jiang, Zhi-Hao Yao, Guo-Qiang Chen
Bacterial morphology is decided by cytoskeleton protein MreB and cell division protein FtsZ encoded by essential genes mreB and ftsZ, respectively. Inactivating mreB and ftsZ lead to increasing cell sizes and cell lengths, respectively, yet seriously reduce cell growth ability. Here we develop a temperature-responsible plasmid expression system for compensated expression of relevant gene(s) in mreB or ftsZ disrupted recombinants H. campaniensis LS21, allowing mreB or ftsZ disrupted recombinants to grow normally at 30°C in a bioreactor for 12h so that a certain cell density can be reached, followed by 36h cell size expansions or cell shape elongations at elevated 37°C at which the mreB and ftsZ encoded plasmid pTKmf failed to replicate in the recombinants and thus lost themselves...
September 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28918258/olfactory-function-in-an-excitotoxic-model-for-secondary-neuronal-degeneration-role-of-dopaminergic-interneurons
#3
Concepció Marin, Sara Laxe, Cristobal Langdon, Joan Berenguer, Eduardo Lehrer, Franklin Mariño-Sánchez, Isam Alobid, Montserrat Bernabeu, Joaquim Mullol
Secondary neuronal degeneration (SND) occurring in Traumatic brain injury (TBI) consists in downstream destructive events affecting cells that were not or only marginally affected by the initial wound, further increasing the effects of the primary injury. Glutamate excitotoxicity is hypothesized to play an important role in SND. TBI is a common cause of olfactory dysfunction that may be spontaneous and partially recovered. The role of the glutamate excitotoxicity in the TBI-induced olfactory dysfunction is still unknown...
September 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28918255/microglia-mediated-baff-baffr-ligation-promotes-neuronal-survival-in-brain-ischemia-injury
#4
Kai Li, Wei Yu, Rangjuan Cao, Zhihua Zhu, Guoqing Zhao
The innate immune responses of brain to vascular occlusion are primarily orchestrated by activated microglia. However, the roles of microglia in inflammatory responses to brain ischemic injuries are controversial. Here, we report a new mechanism by which microglia confer protective effects on ischemic neuronal cells. We found that under ischemic condition, the B-cell activating factor (BAFF) was vastly upregulated in microglia and this upregulation could at least be attributed to JAK-STAT signaling pathway activated by IFN-γ and IL-10, which were spatio-temporally enriched in I/R injured brain as well...
September 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28918078/assessment-of-environmental-impacts-and-operational-costs-of-the-implementation-of-an-innovative-source-separated-urine-treatment
#5
Elorri Igos, Mathilde Besson, Tomás Navarrete Gutiérrez, Ana Barbara Bisinella de Faria, Enrico Benetto, Ligia Barna, Aras Ahmadi, Mathieu Spérandio
Innovative treatment technologies and management methods are necessary to valorise the constituents of wastewater, in particular nutrients from urine (highly concentrated and can have significant impacts related to artificial fertilizer production). The FP7 project, ValuefromUrine, proposed a new two-step process (called VFU) based on struvite precipitation and microbial electrolysis cell (MEC) to recover ammonia, which is further transformed into ammonium sulphate. The environmental and economic impacts of its prospective implementation in the Netherlands were evaluated based on life cycle assessment (LCA) methodology and operational costs...
September 8, 2017: Water Research
https://www.readbyqxmd.com/read/28917860/carbon-materials-derived-from-chitosan-cellulose-cryogel-supported-zeolite-imidazole-frameworks-for-potential-supercapacitor-application
#6
Zehui Li, Lan Yang, Hongbin Cao, Yu Chang, Kexin Tang, Zhiqin Cao, Junjun Chang, Youpeng Cao, Wenbo Wang, Meng Gao, Chenming Liu, Dagang Liu, He Zhao, Yi Zhang, Mingjie Li
In order to promote sustainable development, green and renewable clean energy technologies continue to be developed to meet the growing demand for energy, such as supercapacitor, fuel cells and lithium-ion battery. It is urgent to develop appropriate nanomaterials for these energy technologies to reduce the volume of the device, improve the efficiency of energy conversion and enlarge the energy storage capacity. Here, chitosan/cellulose carbon cryogel (CCS/CCL) were designed and synthesized. Through the introduction of zeolite imidazole frameworks (ZIFs) into the chitosan/cellulose cryogels, the obtained materials showed a microstructure of ZIF-7 (a kind of ZIFs) coated chitosan/cellulose fibers (CS/CL)...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917705/long-term-retention-of-ecm-hydrogel-after-implantation-into-a-sub-acute-stroke-cavity-reduces-lesion-volume
#7
Harmanvir Ghuman, Madeline Gerwig, Francesca J Nicholls, Jessie Liu, Julia Donnelly, Stephen F Badylak, Michel Modo
Salvaging or functional replacement of damaged tissue caused by stroke in the brain remains a major therapeutic challenge. In situ gelation and retention of a hydrogel bioscaffold composed of 8 mg/mL extracellular matrix (ECM) can induce a robust invasion of cells within 24 hours and potentially promote a structural remodeling to replace lost tissue. Herein, we demonstrate a long-term retention of ECM hydrogel within the lesion cavity. A decrease of approximately 32% of ECM volume is observed over 12 weeks...
September 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28917077/pancreatic-%C3%AE-cells-and-type-2-diabetes-development
#8
Miranda-Perez Maria Elizabeth, Alarcon-Aguilar, Francisco J, Ortega-Camarillo Clara, Escobar-Villanueva, Maria Del Carmen
Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia. In particular, type 2 diabetes (T2D) represents one of the main causes of death in the world, and those suffering from it have a lower quality of life. Although there are multiple hypotheses about the pathophysiological mechanisms that lead to the development of T2D, the effects of this pathology on pancreatic β-cells are often ignored. We now know that in addition to genetic defects, β-cell organellar dysfunction participates in the earliest stages of the disease; other factors also contribute to this dysfunction, such as excessive production of reactive oxygen species and a decrease in cellular volume and mass...
2017: Current Diabetes Reviews
https://www.readbyqxmd.com/read/28916996/successive-release-of-tissue-inhibitors-of-metalloproteinase-1-through-graphene-oxide-based-delivery-system-can-promote-skin-regeneration
#9
Cheng Zhong, Dike Shi, Yixiong Zheng, Peter J Nelson, Qi Bao
The purpose of this study was to testify the hypothesis that graphene oxide (GO) could act as an appropriate vehicle for the release of tissue inhibitors of metalloproteinase-1 (TIMP-1) protein in the context of skin repair. GO characteristics were observed by scanning electron microscopy, atomic force microscopy, and thermal gravimetric analysis. After TIMP-1 absorbing GO, the release profiles of various concentrations of TIMP-1 from GO were compared. GO biocompatibility with fibroblast viability was assessed by measuring cell cycle and apoptosis...
September 15, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28916401/cart-cell-free-and-concentrated-ascites-reinfusion-therapy-against-malignancy-related-ascites
#10
REVIEW
Tetsuya Ito, Norio Hanafusa
A standard strategy against ascites, a common symptom observed in cirrhotic and cancer patients, includes restriction of sodium intake and use of a diuretic. Paracentesis is a widely applied method against refractory ascites that do not react to such treatment. However, emerging fatigue and hemodynamic instability are possibly attributable to a loss of protein included in ascites. Cell-free and Concentrated Ascites Reinfusion Therapy (CART) is also applied against refractory ascites. CART comprises three processes...
August 30, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28916388/differential-effects-of-fxr-or-tgr5-activation-in-cholangiocarcinoma-progression
#11
O Erice, I Labiano, A Arbelaiz, A Santos-Laso, P Munoz-Garrido, R Jimenez-Agüero, P Olaizola, A Caro-Maldonado, N Martín-Martín, A Carracedo, E Lozano, J J Marin, C J O'Rourke, J B Andersen, J Llop, V Gómez-Vallejo, D Padro, A Martin, M Marzioni, L Adorini, M Trauner, L Bujanda, M J Perugorria, J M Banales
BACKGROUND AND AIMS: Cholangiocarcinoma (CCA) is an aggressive tumor type affecting cholangiocytes. CCAs frequently arise under certain cholestatic liver conditions. Intrahepatic accumulation of bile acids may facilitate cocarcinogenic effects by triggering an inflammatory response and cholangiocyte proliferation. Here, the role of bile acid receptors FXR and TGR5 in CCA progression was evaluated. METHODS: FXR and TGR5 expression was determined in human CCA tissues and cell lines...
September 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28916346/radiofrequency-ablation-of-t1a-renal-cell-carcinomas-within-renal-transplant-allografts-oncologic-outcomes-and-graft%C3%A2-viability
#12
Derek W Cool, John R Kachura
PURPOSE: To evaluate oncologic outcomes and graft viability after percutaneous RF ablation of renal cell carcinoma (RCC) developing within renal transplant allografts. MATERIALS AND METHODS: A single-institution, retrospective study reviewed all patients treated with RF ablation for RCC between February 2004 and May 2016. Ten patients were identified (age 49.6 y ± 12.6; 9 men, 1 woman) with 12 biopsy-confirmed RCC tumors within the allograft (all T1a, mean diameter 2...
September 13, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/28916142/effect-of-tranexamic-acid-on-gross-hematuria-a-pilot-randomized-clinical-trial-study
#13
Payman Moharamzadeh, Seyedhossein Ojaghihaghighi, Mohsen Amjadi, Farzad Rahmani, Arezoo Farjamnia
OBJECTIVE: Local forms of the tranexamic acid have been effective in treating many haemorrhagic cases. So that the aim of the current study is to assess the effectiveness of local tranexamic acid in controlling painless hematuria in patients referred to the emergency department. METHODS: This is a randomized, double-blind clinical trial study, which was conducted on 50 patients with complaints of painless lower urinary tract bleeding during June 2014 and August 2015...
September 9, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28915721/dramatic-response-of-ctnnb1-and-vegfr-2-mutant-temporal-bone-squamous-cell-carcinoma-to-bevacizumab-in-combination-with-pemetrexed
#14
Lai Wei, Lizhi Wang, Ziye Liu, Meiyi Wang, Weili Lu, Dewei Zhao, Bin Yang, Xuejun Kong, Yan Ding, Zhiqiang Wang
High recurrence rates and poor survival rates for late stage/advanced temporal bone squamous cell carcinoma with the standard treatments continues to be a significant challenge to otolaryngologists. Targeted therapy for temporal bone squamous cell carcinoma after relapse has not been reported. Here we present a 58-year-old man who was diagnosed with recurrent temporal bone squamous cell carcinoma and treated with a regimen developed using whole exome sequencing. Somatic mutations in genes encoding catenin beta 1 and vascular endothelial growth factor receptor 2 were identified in the patient's tumor sample compared to the normal tissue...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915639/heptamethine-carbocyanine-dz-1-dye-for-near-infrared-fluorescence-imaging-of-hepatocellular-carcinoma
#15
Jiaze An, Ningning Zhao, Caiqin Zhang, Yong Zhao, Dengxu Tan, Ya Zhao, Bing Bai, Hai Zhang, Boyang Jason Wu, Changhong Shi
Near-infrared fluorescence (NIRF) dyes have recently emerged as promising tools for non-invasive imaging of different types of cancers. Here, we explored the potential utility of a NIRF DZ-1 dye, with dual imaging and tumour targeting functions, in hepatocellular carcinoma (HCC). We showed the preferential uptake of DZ-1 by HCC cells in vitro and in derived subcutaneous/orthotopic tumour xenografts, accompanied by a minimal effect on normal cells. DZ-1 simplified tumour growth profiling as well, since we were able to correlate NIRF signals with tumour volume and/or tumour-emitting luminescence in mice...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915583/n-acetylcysteine-breaks-resistance-to-trastuzumab-caused-by-muc4-overexpression-in-human-her2-positive-bc-bearing-nude-mice-monitored-by-89-zr-trastuzumab-and-18-f-fdg-pet-imaging
#16
Zéna Wimana, Geraldine Gebhart, Thomas Guiot, Bruno Vanderlinden, Denis Larsimont, Gilles Doumont, Gaetan Van Simaeys, Serge Goldman, Patrick Flamen, Ghanem Ghanem
Trastuzumab remains an important drug in the management of human epidermal growth factor receptor 2 (HER2) overexpressing breast cancer (BC). Several studies reported resistance mechanisms to trastuzumab, including impaired HER2-accessibility caused by mucin 4 (MUC4). Previously, we demonstrated an increase of Zirconium-89-radiolabeled-trastuzumab ((89)Zr-Trastuzumab) accumulation when MUC4-overexpressing BC-cells were challenged with the mucolytic drug N-Acetylcysteine (NAC). Hereby, using the same approach we investigated whether tumor exposure to NAC would also enhance trastuzumab-efficacy...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915507/ambient-fine-particulate-matters-induce-cell-death-and-inflammatory-response-by-influencing-mitochondria-function-in-human-corneal-epithelial-cells
#17
Eun-Jung Park, Jae-Byoung Chae, Jungmook Lyu, Cheolho Yoon, Sanghwa Kim, Changjoo Yeom, Younghun Kim, Jaerak Chang
Ambient fine particulate matter (AFP) is a main risk factor for the cornea as ultraviolet light. However, the mechanism of corneal damage following exposure to AFP has been poorly understood. In this study, we first confirmed that AFP can penetrate the cornea of mice, considering that two-dimensional cell culture systems are limited in reflecting the situation in vivo. Then, we investigated the toxic mechanism using human corneal epithelial (HCET) cells. At 24h after exposure, AFP located within the autophagosome-like vacuoles, and cell proliferation was clearly inhibited in all the tested concentration...
September 12, 2017: Environmental Research
https://www.readbyqxmd.com/read/28915322/hormonal-regulation-of-adipogenesis
#18
Mi-Jeong Lee
Adipose tissue includes multiple anatomical depots that serve as an energy reserve that can expand or contract to maintain metabolic homeostasis. During normal growth and in response to overnutrition, adipose tissue expands by increasing the volume of preexisting adipocytes (hypertrophy) and/or by generating new adipocytes (hyperplasia) via recruitment and differentiation of adipose progenitors. This so-called healthy expansion through hyperplasia is thought to be beneficial in that it protects against obesity associated metabolic disorders by allowing for the "safe" storage of excess energy...
September 12, 2017: Comprehensive Physiology
https://www.readbyqxmd.com/read/28915319/an-improved-model-for-prostate-diffusion-incorporating-the-results-of-monte-carlo-simulations-of-diffusion-in-the-cellular-compartment
#19
Nima Gilani, Paul Malcolm, Glyn Johnson
The purpose of this work was to refine a previously published model of prostate diffusion by incorporating improved estimates of cellular diffusivity obtained by Monte Carlo simulation. Stromal and epithelial cell size and intracellular volume fraction in different grades of cancer were determined from histological images. Diffusion in different mixtures of cells, corresponding to different tumor grades, was simulated and cellular apparent diffusion coefficient and kurtosis values determined. These values were incorporated into the previously published model of prostate diffusion and model predictions compared with values found in the literature...
September 15, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28915312/dce-and-dw-mri-as-early-imaging-biomarkers-of-treatment-response-in-a-preclinical-model-of-triple-negative-breast-cancer
#20
Stephanie L Barnes, Anna G Sorace, Jennifer G Whisenant, J Oliver McIntyre, Hakmook Kang, Thomas E Yankeelov
This work evaluates quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion-weighted MRI (DW-MRI) parameters as early biomarkers of response in a preclinical model of triple negative breast cancer (TNBC). The standard Tofts' model of DCE-MRI returns estimates of the volume transfer constant (K(trans) ) and the extravascular extracellular volume fraction (ve ). DW-MRI returns estimates of the apparent diffusion coefficient (ADC). Mice (n = 38) were injected subcutaneously with MDA-MB-231...
September 15, 2017: NMR in Biomedicine
keyword
keyword
63805
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"