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https://www.readbyqxmd.com/read/29679803/development-of-the-plant-derived-peptide-lunasin-as-an-anticancer-agent
#1
REVIEW
Saleha B Vuyyuri, Chris Shidal, Keith R Davis
The health benefits of soy consumption have long been recognized. An important potential benefit is the linkage of soy consumption with reduced cancer risk. One emerging factor that may confer the anticancer effects of soy is the peptide lunasin. Lunasin has both chemopreventive and therapeutic activities against a variety of carcinogens and cancer types. A novel feature of lunasin is that it contains multiple functional domains that can modulate gene expression through effects on histone acetylation and integrin signaling...
April 18, 2018: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/29679718/identification-of-differential-phosphorylation-and-sub-cellular-localization-of-the-metastasis-suppressor-ndrg1
#2
Kyung Chan Park, Sharleen V Menezes, Danuta S Kalinowski, Sumit Sahni, Patric J Jansson, Zaklina Kovacevic, Des R Richardson
The metastasis suppressor, N-myc downstream regulated gene-1 (NDRG1), exhibits pleiotropic activity, inhibiting metastasis of various tumor-types, while being correlated with metastasis in others. Notably, NDRG1 phosphorylation and cleavage are associated with its function, although it is unclear if these modifications occur universally, or selectively, in different cancer cell-types and if it contributes to its pleiotropy. Considering the suggested DNA repair role of nuclear NDRG1, the effects of the above post-translational modifications on its nuclear localization was examined...
April 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29679667/systemic-delivery-of-bace1-sirna-through-neuron-targeted-nanocomplexes-for-treatment-of-alzheimer-s-disease
#3
Pengzhen Wang, Xiaoyao Zheng, Qian Guo, Peng Yang, Xiaoying Pang, Kang Qian, Wei Lu, Qizhi Zhang, Xinguo Jiang
β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is a key enzyme to cleave the amyloid precursor protein to develop Alzheimer's disease (AD). Reducing BACE1 expression in central neuron through RNA interference technology shows great promise to overcome AD. However, to obtain an efficient and neurons-specific delivery of siRNA against BACE1 through systemic administration remains challenging. Here, we design and prepare siRNA nano-carriers based on PEGylated poly(2-(N,N-dimethylamino) ethyl methacrylate) (PEG-PDMAEMA) modified with both the CGN peptide for blood-brain barrier (BBB) penetration and the Tet1 peptide for neuron-specific binding...
April 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29679610/mutated-mitf-e87r-in-melanoma-enhances-tumor-progression-via-s100a4
#4
Alice Nordlinger, Shani Dror, Abdel Elkahloun, Justine Del Rio, Elisa Stubbs, Tami Golan, Hagar Malcov, Todd D Pricket, Julia C Cronin, Shivang Parikh, Sapir Labes, Laetitia Thomas, Gal Yankovitz, Yuval Tabach, Carmit Levy, Yardena Samuels, Mehdi Khaled
Melanoma, a melanocyte origin neoplasm, is the most lethal type of skin cancer and incidence is increasing. Several familial and somatic mutations have been identified in the gene encoding the melanocyte lineage master regulator, microphthalmia-associated transcription factor (MITF); however, the neoplastic mechanisms of these mutant MITF variants are mostly unknown. Here, by performing unbiased analysis of the transcriptomes in cells expressing mutant MITF, we identified calcium-binding protein S100A4, as a downstream target of MITF-E87R...
April 18, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29679278/overexpression-of-modified-human-tr%C3%AE-1-suppresses-the-growth-of-hepatocarcinoma-sk-hep1-cells-in-vitro-and-in-xenograft-models
#5
Xiaoxiang Peng, Yuntao Zhou, Yanli Sun, Wei Song, Xiangying Meng, Chunling Zhao, Ronglan Zhao
Association studies suggest that TRβ1 functions as a tumor suppressor. Thyroid hormone receptors (TRs) mediate transcriptional responses through a highly conserved DNA-binding domain (DBD). We previously constructed an artificially modified human TRβ1 (m-TRβ1) via the introduction of a 108-bp exon sequence into the corresponding position of the wild-type human TRβ1 (TRβ1) DBD. Studies confirmed that m-TRβ1 was functional and could inhibit the proliferation of breast cancer MDA-MB-468 cells in vitro. To understand the role of m-TRβ1 in liver tumor development, we adopted a gain-of-function approach by stably expressing TRβ (m-TRβ1 and TRβ1) genes in a human hepatocarcinoma cell line, SK-hep1 (without endogenous TRβ), and then evaluated the effects of the expressed TRβ on cancer cell proliferation, migration, and tumor growth in cell-based studies and xenograft models...
April 20, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29679250/the-marine-dinoflagellate-alexandrium-andersoni-induces-cell-death-in-lung-and-colorectal-tumor-cell-lines
#6
Clementina Sansone, Genoveffa Nuzzo, Christian Galasso, Raffaella Casotti, Angelo Fontana, Giovanna Romano, Adrianna Ianora
Dinoflagellates are one of the most important components in marine phytoplankton, second only to diatoms as primary producers. Dinoflagellates have also been reported to produce bioactive secondary metabolites such as polyethers and macrolides with potential applications as pharmaceuticals. Here, we tested the effect of the organic extract and its related enriched extracts from solid-phase extraction (SPE) of a strain of the dinoflagellate Alexandrium andersoni. We found that the SPE extracts induced high cytotoxicity towards two cancer cell lines (A549 lung cancer and HT29 colorectal cancer) without affecting normal cell viability...
April 20, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29678949/hu-antigen-r-regulates-antiviral-innate-immune-responses-through-the-stabilization-of-mrna-for-polo-like-kinase-2
#7
Takuya Sueyoshi, Takumi Kawasaki, Yuichi Kitai, Daisuke Ori, Shizuo Akira, Taro Kawai
Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), RIG-I, and melanoma differentiation-associated gene 5 (MDA5) play a critical role in inducing antiviral innate immune responses by activating IFN regulatory factor 3 (IRF3) and NF-κB, which regulates the transcription of type I IFN and inflammatory cytokines. Antiviral innate immune responses are also regulated by posttranscriptional and translational mechanisms. In this study, we identified an RNA-binding protein HuR as a regulator for RLR signaling...
April 20, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29678919/investigating-the-central-metabolism-of-clostridium-thermosuccinogenes
#8
Jeroen Girwar Koendjbiharie, Kilian Wiersma, Richard van Kranenburg
Clostridium thermosuccinogenes is a thermophilic anaerobic bacterium able to convert various carbohydrates to succinate and acetate as main fermentation products. Genomes of the four publicly available strains have been sequenced and the genome of the type-strain has been closed. The annotated genomes were used to reconstruct the central metabolism, and enzyme assays were used to validate annotations and to determine co-factor specificity. Genes for the pathways to all fermentation products were identified, as well as for the Embden-Meyerhof-Parnas pathway, and the pentose phosphate pathway...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29678569/pbx1-promotes-the-cell-proliferation-via-jak2-stat3-signaling-in-clear-cell-renal-carcinoma
#9
Xin Wei, Lili Yu, Yi Li
PBX1 was abnormally overexpressed and its intracellular localization was found to be frequently amplified in many types of cancer, including renal clear cell carcinoma. PBX1 displays oncogenic activity in several different types of cells, but little is known about how signaling pathways are altered, and the function of PBX1 in renal clear cell carcinoma has not been well investigated. In this study, we demonstrate that the expression of PBX1 was significantly upregulated in 30 pairs of human tissues compared to adjacent normal tissues and the overall survival rate of PBX1-high group was significantly worse than that of PBX1-low group...
April 17, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29678231/incorporation-of-membrane-anchored-flagellin-into-salmonella-gallinarum-bacterial-ghosts-induces-early-immune-responses-and-protection-against-fowl-typhoid-in-young-layer-chickens
#10
Irshad Ahmed Hajam, Je Hyoung Kim, John Hwa Lee
The present study aimed to investigate whether the incorporation of flagellin, a TLR5 agonist, in the bacterial ghosts (BGs) of Salmonella Gallinarum can enhance protective immune responses against fowl typhoid, a septicemic disease of poultry, in chickens. BGs are empty cell envelopes derived from Gram-negative bacteria through the bacteriophage phiX174 gene E mediated lysis. In this study, the S. Gallinarum ghosts carrying flagellin were genetically constructed utilizing a lysis plasmid pJHL184-flagellin, designed for the coexpression of the flagellin and the lysis protein E...
May 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29677358/lysophosphatidic-acid-induces-ecm-production-via-activation-of-the-mechanosensitive-yap-taz-transcriptional-pathway-in-trabecular-meshwork-cells
#11
Leona T Y Ho, Nikolai Skiba, Christoph Ullmer, Ponugoti Vasantha Rao
Purpose: Lysophosphatidic acid (LPA), a bioactive lipid, has been shown to increase resistance to aqueous humor outflow (AH) through the trabecular meshwork (TM). The molecular basis for this response of the TM to LPA, however, is not completely understood. In this study, we explored the possible involvement of mechanosensitive Yes-associated protein (YAP) and its paralog, transcriptional coactivator with PDZ-binding domain (TAZ), transcriptional activation in extracellular matrix (ECM) production by LPA-induced contractile activity in human TM cells (HTM)...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677136/cytoplasmic-translocation-of-nucleolar-protein-nop53-promotes-viral-replication-by-suppressing-host-defense
#12
Wen Meng, Shi-Chong Han, Cui-Cui Li, Hui-Jun Dong, Jian-Yu Chang, Hwa-Chain Robert Wang, Xiao-Jia Wang
NOP53 is a tumor suppressor protein located in the nucleolus and is translocated to the cytoplasm during infection by vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1), as shown in our previous study. Cytoplasmic NOP53 interacts with the retinoic acid-inducible gene I (RIG-I) to remove its K63-linked ubiquitination, leading to attenuation of type I interferon IFN-β. In the present study, we found no obvious translocation of NOP53 in infection by a mutant virus lacking ICP4 (HSV-1/d120, replication inadequate)...
April 20, 2018: Viruses
https://www.readbyqxmd.com/read/29676849/hoxc10-promotes-proliferation-and-invasion-and-induces-immunosuppressive-gene-expression-in-glioma
#13
Shu Li, Wenhao Zhang, Chao Wu, Hongliang Gao, Jun Yu, Xiaoqiang Wang, Bin Li, Jun Zhong, Wenchaun Zhang, Ping Zhou, Juanhong Shi, Lifeng Wang, Yunxing Gao, Shiting Li, Bangbao Tao
The prognosis for patients with malignant glioma is very poor and thus the identification of new potential therapeutic targets is critically important. In this work, we report a previously unknown role for the homeobox transcription factor HOXC10 in regulating immunosuppressive gene expression in glioma cell lines, as well as their proliferative and invasive capacities. Although HOXC10 expression is dysregulated in several types of tumors, its potential function in glioma was not known. We found that HOXC10 expression was up-regulated in glioma compared to normal tissue, and that HOXC10 expression positively associated with high grading of glioma...
April 20, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29676620/interleukin-13-stimulation-reveals-the-cellular-and-functional-plasticity-of-the-airway-epithelium
#14
Max A Seibold
About 50% of patients with asthma exhibit chronic airway inflammation driven by the type 2 cytokines interleukin (IL)-4, IL-5, and IL-13. These patients with type 2-high asthma experience more allergic symptoms, greater airway hyperresponsiveness, and more severe mucus obstruction than patients with type 2-low asthma. Mouse models of asthma have shown that much of the airway dysfunction in these models can be generated by IL-13 stimulation of the airway epithelium alone. Both in vivo mouse model studies and in vitro studies of human mucociliary airway epithelial cultures have shown that IL-13 induces cellular remodeling of the airway epithelium through proliferation-independent transdifferentiation processes...
April 2018: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29676227/chemically-modified-mrna-modified-rna-application-for-treatment-of-achilles-tendon-defects
#15
Elmar Herbst, Florian Imhoff, Peter Föhr, Stefan Milz, Christian Plank, Carsten Rudolph, Günther Hasenpusch, Johannes Geiger, Manish Aneja, Karoline Groth, Stephan Vogt, Andreas Imhoff, Andreas Schmitt
Different regenerative medicine approaches for tendon healing exist. Recently, especially gene therapy gained popularity. However, potential mutagenic and immunologic effects might prevent from translation to clinical research. Chemically modified mRNA (cmRNA) might bypass these limitations of gene therapy. Therefore, the purpose of this study was to evaluate the early healing properties of Achilles tendon defects in rats treated with basic fibroblast growth factor (bFGF) cmRNA. Forty male Lewis rats were used for the study and randomly assigned to the two study groups: 1) treatment with cmRNA coding for bFGF, 2) non-coding cmRNA control...
April 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29675560/patterns-of-differential-gene-expression-in-a-cellular-model-of-human-islet-development-and-relationship-to-type-2-diabetes-predisposition
#16
Marta Perez-Alcantara, Christian Honoré, Agata Wesolowska-Andersen, Anna L Gloyn, Mark I McCarthy, Mattias Hansson, Nicola L Beer, Martijn van de Bunt
AIMS/HYPOTHESIS: Most type 2 diabetes-associated genetic variants identified via genome-wide association studies (GWASs) appear to act via the pancreatic islet. Observed defects in insulin secretion could result from an impact of these variants on islet development and/or the function of mature islets. Most functional studies have focused on the latter, given limitations regarding access to human fetal islet tissue. Capitalising upon advances in in vitro differentiation, we characterised the transcriptomes of human induced pluripotent stem cell (iPSC) lines differentiated along the pancreatic endocrine lineage, and explored the contribution of altered islet development to the pathogenesis of type 2 diabetes...
April 19, 2018: Diabetologia
https://www.readbyqxmd.com/read/29675490/evaluation-of-changes-in-the-expression-of-wnt-%C3%AE-catenin-target-genes-in-mouse-reproductive-tissues-during-estrous-cycle-an-experimental-study
#17
Saeed Zavareh, Zahara Gholizadeh, Taghi Lashkarbolouki
Background: The Wingless-type (Wnt)/β-catenin signaling pathway controls cell homeostasis. Reproductive tissues are dynamic in response to steroidal hormone changes. Steroidal hormones are known to control the Wnt/β-catenin pathway, but their role in reproductive tissues remains unknown. Objective: The present study aims to investigate the expression patterns of Wnt/β-catenin target genes in mouse reproductive tissues during the normal estrous cycle. Materials and Methods: In this experimental study, 16 adult NMRI mice were grouped as proestrus, estrus, metestrus, and diestrus according to vaginal smear and histological evaluation of uterine and ovarian tissues...
February 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29675407/pancreatic-aquaporin-7-a-novel-target-for-anti-diabetic-drugs
#18
REVIEW
Leire Méndez-Giménez, Silvia Ezquerro, Inês V da Silva, Graça Soveral, Gema Frühbeck, Amaia Rodríguez
Aquaporins comprise a family of 13 members of water channels (AQP0-12) that facilitate a rapid transport of water across cell membranes. In some cases, these pores are also permeated by small solutes, particularly glycerol, urea or nitric oxide, among other solutes. Several aquaporins have been identified in the pancreas, an exocrine and endocrine organ that plays an essential role in the onset of insulin resistance and type 2 diabetes. The exocrine pancreas, which accounts for 90% of the total pancreas, secretes daily large volumes of a near-isotonic fluid containing digestive enzymes into the duodenum...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29675399/sialidase-deficiency-in-porphyromonas-gingivalis-increases-il-12-secretion-in-stimulated-macrophages-through-regulation-of-cr3-incrna-gas5-and-mir-21
#19
Xue Yang, Yaping Pan, Xiaoyu Xu, Tong Tong, Shiwen Yu, Yue Zhao, Li Lin, Jingbo Liu, Dongmei Zhang, Chen Li
Porphyromonas gingivalis ( P. gingivalis ) is a major periodontal pathogen that can induce an immune response leading to a destructive inflammatory process. During the inflammatory process, interleukin-12 (IL-12) is secreted, correlating with bacterial clearance by macrophages. Bacterial sialidase has recently been shown to influence the synthesis and modification of the macromolecules on its surface, and is associated with the interaction between bacteria and host cells. We have previously constructed a P...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29675003/role-of-micrornas-and-exosomes-in-helicobacter-pylori-and-epstein-barr-virus-associated-gastric-cancers
#20
REVIEW
Iva Polakovicova, Sofia Jerez, Ignacio A Wichmann, Alejandra Sandoval-Bórquez, Nicolás Carrasco-Véliz, Alejandro H Corvalán
Emerging evidence suggests that chronic inflammation caused by pathogen infection is connected to the development of various types of cancer. It is estimated that up to 20% of all cancer deaths is linked to infections and inflammation. In gastric cancer, such triggers can be infection of the gastric epithelium by either Helicobacter pylori ( H. pylori ), a bacterium present in half of the world population; or by Epstein-Barr virus (EBV), a double-stranded DNA virus which has recently been associated with gastric cancer...
2018: Frontiers in Microbiology
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