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https://www.readbyqxmd.com/read/29471534/cypermethrin-promotes-lung-cancer-metastasis-via-modulation-of-macrophage-polarization-by-targeting-microrna-155-bcl6
#1
Fang Huang, Zhifeng Chen, Hanwen Chen, Wei Lu, Shujun Xie, Qing H Meng, Yihua Wu, Dajing Xia
Cypermethrin (CYM), a type II pyrethroid, is widely used as an insecticide for agriculture and household. Cumulative evidence indicates that acute and chronic exposure to CYM might cause a number of health problems, such as cancer and neuronal system diseases. However, the molecular mechanism underlying this pathology is not known. The main objective of our study was to define the effects of CYM on macrophages and the implication of such effects in cancer metastasis and the potential mechanism involved. The effects of CYM on the macrophages were evaluated by detecting the expression of M1 and M2 macrophage polarization markers through ELISA, quantitative RT-PCR and Western blot assay...
February 19, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29471425/foxl2c134w-induced-cyp19-expression-via-cooperation-with-smad3-in-hgrc1-cells
#2
Martina Belli, Nahoko Iwata, Tomoko Nakamura, Akira Iwase, Dwayne Stupack, Shunichi Shimasaki
Germline knockout studies in female mice demonstrated an essential role for FOXL2 in early follicle development, while an inducible granulosa cell (GC) specific deletion of Foxl2 in adults has shown ovary-to-testis somatic sex reprogramming. In women, over 120 different germline mutations in the FOXL2 gene have been shown to cause blepharophimosis/ptosis/epicantus inversus syndrome associated with or without primary ovarian insufficiency. By contrast, a single somatic mutation (FOXL2C134W) accounts for almost all adult-type GC tumors (aGCTs)...
February 19, 2018: Endocrinology
https://www.readbyqxmd.com/read/29471289/single-nucleotide-polymorphism-facilitated-down-regulation-of-the-cohesin-stromal-antigen-1-implications-for-colorectal-cancer-racial-disparities
#3
Somenath Datta, Richard M Sherva, Mart De La Cruz, Michelle T Long, Priya Roy, Vadim Backman, Sanjib Chowdhury, Hemant K Roy
The biological underpinnings for racial disparities in colorectal cancer (CRC) incidence remain to be elucidated. We have previously reported that the cohesin SA-1 down-regulation is an early event in colon carcinogenesis which is dramatically accentuated in African-Americans. In order to investigate the mechanism, we evaluated single nucleotide polymorphisms (SNPs) for association with SA-1-related outcomes followed by gene editing of candidate SNP. We observed that rs34149860 SNP was significantly associated with a lower colonic mucosal SA-1 expression and evaluation of public databases showed striking racial discordance...
February 19, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29470985/cytoplasmic-p53-contributes-to-the-removal-of-uracils-misincorporated-by-hiv-1-reverse-transcriptase
#4
Yossi Saragani, Amnon Hizi, Galia Rahav, Sara Zauch, Mary Bakhanashvili
HIV-1 reverse transcriptase (RT) in the cytoplasm of HIV-infected cells efficiently inserts the non-canonical dUTP into the proviral DNA, and extends the dU-terminated DNA. The misincorporation of dUTP leads to mutagenesis, and uracils can down-regulate viral gene expression. However, uracilation might also protect HIV DNA from auto-integration in the cytoplasm. Tumor suppressor p53 protein, exhibiting inherent 3'→5' exonuclease activity, provides a potential host-derived repair mechanism during HIV reverse transcription for the misincorporation of various wrong nucleotides, leading to both base-base mismatches and incorporated non-canonical ribonucleotides...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29470839/embryonic-neurogenesis-in-echinoderms
#5
REVIEW
Veronica F Hinman, Robert D Burke
The phylogenetic position of echinoderms is well suited to revealing shared features of deuterostomes that distinguish them from other bilaterians. Although echinoderm neurobiology remains understudied, genomic resources, molecular methods, and systems approaches have enabled progress in understanding mechanisms of embryonic neurogenesis. Even though the morphology of echinoderm larvae is diverse, larval nervous systems, which arise during gastrulation, have numerous similarities in their organization. Diverse neural subtypes and specialized sensory neurons have been identified and details of neuroanatomy using neuron-specific labels provide hypotheses for neural function...
February 22, 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29470824/turning-up-the-heat-an-evaluation-of-the-evidence-for-heating-to-promote-exercise-recovery-muscle-rehabilitation-and-adaptation
#6
REVIEW
Hamish McGorm, Llion A Roberts, Jeff S Coombes, Jonathan M Peake
Historically, heat has been used in various clinical and sports rehabilitation settings to treat soft tissue injuries. More recently, interest has emerged in using heat to pre-condition muscle against injury. The aim of this narrative review was to collate information on different types of heat therapy, explain the physiological rationale for heat therapy, and to summarise and evaluate the effects of heat therapy before, during and after muscle injury, immobilisation and strength training. Studies on skeletal muscle cells demonstrate that heat attenuates cellular damage and protein degradation (following in vitro challenges/insults to the cells)...
February 22, 2018: Sports Medicine
https://www.readbyqxmd.com/read/29470700/incidental-diagnosis-of-parathyroid-lesions-by-preoperative-use-of-next-generation-molecular-testing
#7
Wu Bo, Karen E Schoedel, Sally E Carty, Lisa A Radkay, N Paul Ohori, Yuri E Nikiforov, Marina N Nikiforova, Linwah Yip
BACKGROUND: Parathyroid glands can mimic thyroid follicular lesions on fine-needle aspiration (FNA) cytology and can lead to unnecessary or incorrect surgery. Newer molecular panel tests using next-generation sequencing (NGS) include analysis of cell type-specific gene expression profiles such as parathyroid. The study aim is to determine the frequency and clinical implications of parathyroid tissue identification by molecular testing in cytologically indeterminate "thyroid" lesions...
February 22, 2018: World Journal of Surgery
https://www.readbyqxmd.com/read/29470680/changes-in-skeletal-muscle-and-body-weight-on-sleeping-beauty-transposon-mediated-transgenic-mice-overexpressing-pig-migf-1
#8
Bo Gao, Wei Wang, Han Wu, Cai Chen, Dan Shen, Saisai Wang, Wei Chen, Li Zhang, Shuheng Chan, Chengyi Song
Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo and to investigate the molecular mechanisms, the transgenic mouse model was generated which can also provide experimental data for making transgenic pigs with pig endogenous IGF1 gene. We constructed a skeletal muscle-specific expression vector using 5'- and 3'-regulatory regions of porcine skeletal α-actin gene...
February 22, 2018: Biochemical Genetics
https://www.readbyqxmd.com/read/29470493/effective-polyploidy-causes-phenotypic-delay-and-influences-bacterial-evolvability
#9
Lei Sun, Helen K Alexander, Balazs Bogos, Daniel J Kiviet, Martin Ackermann, Sebastian Bonhoeffer
Whether mutations in bacteria exhibit a noticeable delay before expressing their corresponding mutant phenotype was discussed intensively in the 1940s to 1950s, but the discussion eventually waned for lack of supportive evidence and perceived incompatibility with observed mutant distributions in fluctuation tests. Phenotypic delay in bacteria is widely assumed to be negligible, despite the lack of direct evidence. Here, we revisited the question using recombineering to introduce antibiotic resistance mutations into E...
February 22, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29469991/gene-expression-on-dna-biochips-patterned-with-strand-displacement-lithography
#10
Günther Pardatscher, Matthaeus Schwarz-Schilling, Shirley S Daube, Roy H Bar-Ziv, Friedrich Christian Simmel
Lithographic patterning of DNA molecules enables spatial organization of cell-free genetic circuits under well-controlled experimental conditions. Here, we present a biocompatible, DNA-based resist termed "Bephore" based on commercially available components, which can be patterned by both photo- and electron beam lithography. The patterning mechanism is based on cleavage of a chemically modified DNA hairpin by ultraviolet light or electrons, and a subsequent strand displacement reaction. All steps are performed in aqueous solution and do not require chemical development of the resist, which makes the lithographic process robust and biocompatible...
February 22, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29469614/tgf-%C3%AE-1-induced-deposition-of-provisional-extracellular-matrix-by-tracheal-basal-cells-promotes-epithelial-to-mesenchymal-transition-in-a-jnk1-dependent-manner
#11
Jos L van der Velden, Darcy E Wagner, Karolyn G Lahue, Sarah T Abdalla, Ying W Lam, Daniel J Weiss, Yvonne M W Janssen-Heininger
Epithelial cells have been suggested as potential drivers of lung fibrosis, although the epithelial-dependent pathways that promote fibrogenesis remain unknown. Extracellular matrix is increasingly recognized as an environment that can drive cellular responses in various pulmonary diseases. In this study, we demonstrate that transforming growth factor- beta (TGF-β1)-stimulated mouse tracheal basal (MTB) cells produce provisional matrix proteins in vitro, which initiate mesenchymal changes in subsequently freshly plated MTB cells via Rho kinase- and c-Jun-N-terminal kinase (JNK1)-dependent processes...
February 22, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29469612/the-atypical-chemokine-receptor-ackr2-drives-pulmonary-fibrosis-by-tuning-influx-of-ccr2-and-ccr5-ifn%C3%AE-producing-%C3%AE-%C3%AE-t-cells-in-mice
#12
Remo Castro Russo, Benedetta Savino, Massimiliano Mirolo, Chiara Buracchi, Giovanni Germano, Achille Anselmo, Luca Zammataro, Fabio Pasqualini, Alberto Mantovani, Massimo Locati, Mauro M Teixeira
RATIONALE: Chemokines coordinate lung inflammation and fibrosis by acting on chemokine receptors expressed on leukocytes and other cell types. Atypical chemokine receptors (ACKRs) bind, internalize and degrade chemokines, tuning homeostasis and immune responses. ACKR2 recognizes and decreases levels of inflammatory CC chemokines. The role of ACKR2 in fibrogenesis is unknown. OBJECTIVE: Investigate the role of ACKR2 in the context of pulmonary fibrosis. METHODS: The effects of ACKR2 expression and deficiency during inflammation and fibrosis were analyzed using a bleomycin-model of fibrosis, ACKR2-deficient mice, bone marrow chimeras and antibody-mediated leukocyte depletion...
February 22, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29468963/nf-%C3%AE%C2%BAb-ikk%C3%AE-pathway-as-a-target-for-drug-development-realities-challenges-and-perspectives
#13
Rosana H C N Freitas, Carlos A M Fraga
BACKGROUND: Nuclear factor κB (NF-κB) comprises a family of proteins that act as transcription factors promoting the expression of many genes. Activation of NF-κB biochemical cascades is associated with the regulation of innate and adaptive immune responses and inflammation, among other physiological responses. However, genetic abnormalities and continuous stimulation of the NF-κB-IKKβ pathway are directly related to many types of inflammatory and autoimmune diseases, as well as to the genesis and survival of tumor cells...
February 19, 2018: Current Drug Targets
https://www.readbyqxmd.com/read/29468299/smad4-and-epithelial-mesenchymal-transition-proteins-in-colorectal-carcinoma-an-immunohistochemical-study
#14
M Ioannou, E Kouvaras, R Papamichali, M Samara, I Chiotoglou, G Koukoulis
Epithelial-mesenchymal transition (EMT) plays an important role in cancer metastasis. During EMT, tumor cells acquire the capacity to migrate and invade the stroma. Activation of the transforming growth factor-b (TGF-b) signaling pathway is of major importance for the initiation of EMT. Smad4, an essential protein of this pathway, is known to complex with multiple transcription factors (e.g. Snail-1, Slug, Twist-1), in various types of cancer, promoting the repression or activation of target genes. The role of Smad4 in colorectal cancer (CRC) is not straightforward so far...
February 21, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29467930/identification-of-the-potential-crucial-genes-in-invasive-ductal-carcinoma-using-bioinformatics-analysis
#15
Chunguang Li, Liangtao Luo, Sheng Wei, Xiongbiao Wang
Invasive ductal carcinoma (IDC) is a common histological type of breast cancer. The aim of this study was to identify the potential crucial genes associated with IDC and to provide valid biological information for further investigations. The gene expression profiles of GSE10780 which contained 42 histologically normal breast tissues and 143 IDC tissues were downloaded from the GEO database. Functional and pathway enrichment analysis of differentially expressed genes (DEGs) were performed and protein-protein interaction (PPI) network was analyzed using Cytoscape...
January 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29467901/ubiquitin-specific-protease-15-promotes-tumor-cell-invasion-and-proliferation-in-glioblastoma
#16
Ke Xu, Hua Pei, Zhenhao Zhang, Huamin Wang, Liang Li, Qianfeng Xia
Glioblastoma multiforme (GBM) is one of the most aggressive types of brain tumor worldwide. Despite the advances made in treatment and research, the median survival time for GBM patients remains <1.5 years, providing impetus for the identification of potential novel therapeutic target genes to improve GBM treatment. The deubiquitinating enzyme ubiquitin specific peptidase 15 (USP15) has emerged as a pro-oncogenic factor; however, its function in GBM has yet to be fully elucidated. The present study sought to determine whether or not USP15 is implicated in GBM cell invasion and proliferation...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29467875/potential-prognostic-biomarkers-identified-by-dna-methylation-profiling-analysis-for-patients-with-lung-adenocarcinoma
#17
Liankui Han, Gang Xu, Chuan Xu, Bo Liu, Di Liu
Lung adenocarcinoma is frequently occurring type of lung cancer with high metastatic risk. We performed a DNA methylation profiling analysis to identify possible prognostic markers involved in lung adenocarcinoma. DNA methylation profiling data (GSE66386) were downloaded from the Gene Expression Omnibus (GEO) database. Differentially methylated genes were identified using a limma package. GO enrichment analysis was performed to identify vital functions related to differential gene methylation, and pathway analysis was performed to assess the associations between different proteins with regard to regulation of cell function and metabolism...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29467773/characterization-of-novel-plant-symbiosis-mutants-using-a-new-multiple-gene-expression-reporter-sinorhizobium-meliloti-strain
#18
Claus Lang, Lucinda S Smith, Sharon R Long
The formation of nitrogen fixing root nodules by Medicago truncatula and Sinorhizobium meliloti requires communication between both organisms and coordinated differentiation of plant and bacterial cells. After an initial signal exchange, the bacteria invade the tissue of the growing nodule via plant-derived tubular structures, called infection threads. The bacteria are released from the infection threads into invasion-competent plant cells, where they differentiate into nitrogen-fixing bacteroids. Both organisms undergo dramatic transcriptional, metabolic and morphological changes during nodule development...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29467657/effects-of-sodium-and-amino-acid-substrate-availability-upon-the-expression-and-stability-of-the-snat2-slc38a2-amino-acid-transporter
#19
Thorsten M Hoffmann, Emma Cwiklinski, Dinesh S Shah, Clare Stretton, Russell Hyde, Peter M Taylor, Harinder S Hundal
The SNAT2 (SLC38A2) System A amino acid transporter mediates Na+ -coupled cellular uptake of small neutral α-amino acids (AAs) and is extensively regulated in response to humoral and nutritional cues. Understanding the basis of such regulation is important given that AA uptake via SNAT2 has been linked to activation of mTORC1; a major controller of many important cellular processes including, for example, mRNA translation, lipid synthesis, and autophagy and whose dysregulation has been implicated in the development of cancer and conditions such as obesity and type 2 diabetes...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29467617/quantitative-proteomics-of-synaptosomal-fractions-in-a-rat-overexpressing-human-disc1-gene-indicates-profound-synaptic-dysregulation-in-the-dorsal-striatum
#20
Fernando J Sialana, An-Li Wang, Benedetta Fazari, Martina Kristofova, Roman Smidak, Svenja V Trossbach, Carsten Korth, Joseph P Huston, Maria A de Souza Silva, Gert Lubec
Disrupted-in-schizophrenia 1 (DISC1) is a key protein involved in behavioral processes and various mental disorders, including schizophrenia and major depression. A transgenic rat overexpressing non-mutant human DISC1, modeling aberrant proteostasis of the DISC1 protein, displays behavioral, biochemical and anatomical deficits consistent with aspects of mental disorders, including changes in the dorsal striatum, an anatomical region critical in the development of behavioral disorders. Herein, dorsal striatum of 10 transgenic DISC1 (tgDISC1) and 10 wild type (WT) littermate control rats was used for synaptosomal preparations and for performing liquid chromatography-tandem mass spectrometry (LC-MS)-based quantitative proteomics, using isobaric labeling (TMT10plex)...
2018: Frontiers in Molecular Neuroscience
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