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https://www.readbyqxmd.com/read/27904014/male-infertility-related-molecules-involved-in-sperm-oocyte-fusion
#1
Lisha Mou, Ni Xie
Male infertility has become a very serious problem in the human reproduction system, but the molecular mechanism of infertility remains largely unknown. Fertilization is the phenomenon in which a sperm and oocyte find each other, interact, and fuse. Sperm-oocyte fusion-related factors on the sperm side play crucial roles in male infertility. For example, IZUMO1 is well-known as a sperm protein essential for fusion of a sperm and oocyte, but its dysfunction or mutation can result in male infertility. Recent studies showed a novel sperm protein named Bactericidal/permeability-increasing protein (BPI), which takes part in the sperm-oocyte fusion process...
December 1, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27898841/rapid-communication-nerve-growth-factor-influences-cleavage-rate-and-embryo-development-in-sheep
#2
M Crispo, P C Dos Santos-Neto, M Vilariño, A P Mulet, A de León, L Barbeito, A Menchaca
Recent information about Nerve growth factor (NGF), a protein traditionally associated to the nervous system that regulates survival and maturation of developing neurons, suggests that it may exert action also on different levels in the reproductive system. The aim of this study was to evaluate the effect of NGF added during in vitro oocyte maturation, fertilization or in vitro embryo development in sheep. Nerve growth factor was supplemented to the culture medium at 0, 100, or 1,000 ng/mL, during either in vitro maturation (Exp...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27896535/generation-of-gene-edited-birds-in-one-generation-using-sperm-transfection-assisted-gene-editing-stage
#3
Caitlin A Cooper, Arjun Challagulla, Kristie A Jenkins, Terry G Wise, Terri E O'Neil, Kirsten R Morris, Mark L Tizard, Timothy J Doran
Generating transgenic and gene edited mammals involves in vitro manipulation of oocytes or single cell embryos. Due to the comparative inaccessibility of avian oocytes and single cell embryos, novel protocols have been developed to produce transgenic and gene edited birds. While these protocols are relatively efficient, they involve two generation intervals before reaching complete somatic and germline expressing transgenic or gene edited birds. Most of this work has been done with chickens, and many protocols require in vitro culturing of primordial germ cells (PGCs)...
November 28, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27895531/follicular-fluid-biomarkers-for-human-in-vitro-fertilization-outcome-proof-of-principle
#4
Fang Chen, Carl Spiessens, Thomas D'Hooghe, Karen Peeraer, Sebastien Carpentier
BACKGROUND: Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported. METHODS: This study screened FF for peptide biomarkers that predict the outcome of in vitro fertilization (IVF)...
2016: Proteome Science
https://www.readbyqxmd.com/read/27894151/value-of-the-ultrasound-in-the-study-of-ovarian-reserve-for-prediction-of-oocyte-recovery
#5
Meire Spressão, Antonio Hélio Oliani, Denise Cristina Mós Vaz Oliani
Purpose To identify which methods used in the assessment of the ovarian reserve are exclusive or complementary to identify the best response to follicle development. Methods Retrospective cohort study, involving patients undergoing assisted reproduction treatment at the Instituto de Medicina Reprodutiva e Fetal, from April 2009 to July 2014. Age, biochemical tests, and ultrasound were assessed. The data were analyzed to predict the follicular development and the relation between them, using, for statistical analysis, Statistical Package for Social Sciences software...
October 2016: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/27881734/nlrp2-controls-age-associated-maternal-fertility
#6
Anna A Kuchmiy, Jinke D'Hont, Tino Hochepied, Mohamed Lamkanfi
Nucleotide-binding domain and leucine-rich repeat (NLR) proteins are well-known for their key roles in the immune system. Ectopically expressed NLRP2 in immortalized cell lines assembles an inflammasome and inhibits activation of the proinflammatory transcription factor NF-κB, but the physiological roles of NLRP2 are unknown. Here, we show that Nlrp2-deficient mice were born with expected Mendelian ratios and that Nlrp2 was dispensable for innate and adaptive immunity. The observation that Nlrp2 was exclusively expressed in oocytes led us to explore the role of Nlrp2 in parthenogenetic activation of oocytes...
November 23, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27879344/failure-to-launch-aberrant-cumulus-gene-expression-during-oocyte-in-vitro-maturation
#7
Hannah Mary Brown, Kylie Dunning, Melanie Sutton-McDowall, Rob B Gilchrist, Jeremy G Thompson, Darryl L Russell
In vitro maturation (IVM) offers significant benefits for human infertility treatment and animal breeding, but this potential is yet to be fully realised due to reduced oocyte developmental competence in comparison with in vivo matured oocytes. Cumulus cells occupy an essential position in determining oocyte developmental competence. Here we have examined the areas of deficient gene expression, as determined within microarrays primarily from cumulus cells of mouse COCs, but also other species, between in vivo matured and in vitro matured oocytes...
November 22, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27878881/beneficial-effect-of-extracellular-adenosine-5-triphosphate-treatment-on-the-indochinese-leopard-panthera-pardus-delacouri-sperm-quality-after-cryopreservation
#8
P Thuwanut, W Tipkantha, B Siriaroonrat, P Comizzoli, K Chatdarong
The Indochinese leopard (Panthera pardus delacouri) population, included in CITES Appendix I, has been declining for decades. Proper gamete preservation condition is critical for breeding programme management using artificial insemination or in vitro fertilization (IVF). The present study aimed at investigating the impact of post-thawing treatment of leopard semen with extracellular adenosine 5'-triphosphate (ATPe) on sperm quality (including morphological traits and ability to fertilize an oocyte). Semen from six adult male leopards was collected by electroejaculation (one ejaculation per cat)...
November 22, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27871901/the-immunomodulation-of-a-maternal-translationally-controlled-tumor-protein-tctp-in-zhikong-scallop-chlamys-farreri
#9
Zhihao Jia, Mengqiang Wang, Feng Yue, Xiudan Wang, Lingling Wang, Linsheng Song
Translationally controlled tumor protein (TCTP) is initially described as a highly conserved protein implicated in cell growth, and it is subsequently confirmed to play important roles in mediating the innate immune response, especially the inflammatory. In the present study, the full-length cDNA sequence of a TCTP from Zhikong scallop Chlamys farreri (designed as CfTCTP) was cloned by rapid amplification of cDNA ends (RACE) technique based on the expression sequence tag (EST) analysis. It was of 1230 bp with an open reading frame (ORF) of 543 bp encoding a polypeptide of 180 amino acids...
November 18, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27864653/the-developmental-competence-of-oocytes-parthenogenetically-activated-by-an-electric-pulse-and-anisomycin-treatment
#10
Yu-Chen Zhang, Long Jin, Hai-Ying Zhu, Qing Guo, Xiao-Chen Li, Guang-Lei Zhang, Xiao-Xu Xing, Mei-Fu Xuan, Qi-Rong Luo, Zhao-Bo Luo, Jun-Xia Wang, Cheng-Du Cui, Wen-Xue Li, Zheng-Yun Cui, Xi-Jun Yin, Jin-Dan Kang
OBJECTIVE: The aim of this study was to investigate the developmental competence of oocytes parthenogenetically activated by an electric pulse (EP) and treated with anisomycin and to determine whether this method is applicable to somatic cell nuclear transfer (SCNT). RESULTS: Embryos derived from porcine oocytes parthenogenetically activated by an EP and treatment with 0.01 µg/mL anisomycin had a significantly improved in vitro developmental capacity. Furthermore, 66...
November 18, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27862569/fibroblast-growth-factor-10-markedly-improves-in-vitro-maturation-of-porcine-cumulus-oocyte-complexes
#11
Yeo-Jin Son, Seung-Eun Lee, Hyuk Hyun, Min-Young Shin, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Growth factors synthesized by ovarian somatic cells affect cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10), for example, is a known regulator of mammalian cumulus-oocyte complex maturation. In this study, we investigated the effects of 0, 5, 10, 50, and 100 ng/mL FGF10 (5F, 10F, 50F, and 100F, respectively) on in vitro cumulus cell expansion, oocyte maturation, and embryo development. The percentage of fully expanded cumulus cells at the oocyte's metaphase-II (MII) stage was significantly higher in the 10F-treated group than in the control...
November 11, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27862395/the-nuclear-and-developmental-competence-of-cumulus-oocyte-complexes-is-enhanced-by-three-dimensional-coculture-with-conspecific-denuded-oocytes-during-in-vitro-maturation-in-the-domestic-cat-model
#12
M G Morselli, G C Luvoni, P Comizzoli
The objective of the study was to assess the efficacy of coculture with conspecific cumulus-denuded oocytes (CDOs) during in vitro maturation in a three-dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus-oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of in vitro maturation, before assessing the meiotic progression. In Experiment II, the in vitro fertilization of COCs and CDOs was carried out with chilled epididymal spermatozoa and the presumptive zygotes were cultured in vitro separately for 7 days in 3D microcapsules before assesment of embryonic development...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27856326/expression-pattern-analysis-of-irf4-and-its-related-genes-revealed-the-functional-differentiation-of-irf4-paralogues-in-teleost
#13
Kete Ai, Kai Luo, Youshen Li, Wei Hu, Weihua Gao, Liu Fang, Guangming Tian, Guoliang Ruan, Qiaoqing Xu
In mammals, interferon regulatory factor 4 (IRF4) plays an important role in the process of development and differentiation of B cells, T cells and dendritic cells. It can regulate immune pathway through IRF5, MyD88, IL21, PGC1α, and NOD2. In the present study, we investigated the expression pattern of IRF4 paralogues and these related genes for the first time in teleosts. The results showed that these genes were all expressed predominantly in known immune tissues while IRF5 was also relatively highly expressed in muscle...
November 14, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27852688/the-role-of-intracytoplasmic-sperm-injection-in-non-male-factor-infertility-in-advanced-maternal-age
#14
Samer Tannus, Weon-Young Son, Ashley Gilman, Grace Younes, Tal Shavit, Michael-Haim Dahan
STUDY QUESTION: Does ICSI improve reproductive outcomes compared with conventional IVF when used for non-male factor infertility in women aged 40 years and over? SUMMARY ANSWER: There is no advantage of ICSI over conventional IVF in women aged 40 years and over when used for non-male factor infertility. WHAT IS KNOWN ALREADY: The use of ICSI has increased dramatically in recent years and is being applied for indications other than male factor infertility...
November 16, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27851888/regulation-of-heat-inducible-hspa1a-gene-expression-during-maternal-to-embryo-transition-and-in-response-to-heat-in-in-vitro-produced-bovine-embryos
#15
Jean-Marc Lelièvre, Nathalie Peynot, Sylvie Ruffini, Ludivine Laffont, Daniel Le Bourhis, Pierre-Marie Girard, Véronique Duranthon
In in vitro-produced (IVP) bovine embryos, a burst in transcriptional activation of the embryonic genome (EGA) occurs at the 8-16-cell stage. To examine transcriptional regulation prior to EGA, notably in response to heat stress, we asked (1) whether the spontaneous expression of a luciferase transgene that is driven by the minimal mouse heat-shock protein 1b (hspa1b) gene promoter paralleled that of HSPA1A during EGA in IVP bovine embryo and (2) whether expression of the endogenous heat-inducible iHSPA group member HSPA1A gene and the hspa1b/luciferase transgene were induced by heat stress (HS) prior to EGA...
November 17, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27834314/the-effect-of-collagen-hydrogel-on-3d-culture-of-ovarian-follicles
#16
Sunyoung Joo, Seh-Hoon Oh, Sivanandane Sittadjody, Emmanual C Opara, John D Jackson, Sang Jin Lee, James J Yoo, Anthony Atala
The in vivo function and phenotype of ovarian follicle cells are determined by many factors. When these cells are removed from the in vivo microenvironment and grown in a 2D in vitro environment, the function of the follicular cells is difficult to preserve. A collagen hydrogel was used to examine the hormone and oocyte maturation of ovary follicles in a 3D culture system. Ovarian follicles from rats were isolated and cultured in various concentration of type I collagen hydrogels ranging from 1% to 7% (weight/volume)...
November 11, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27833987/pregnancy-resulting-from-imsi-after-testicular-biopsy-in-a-patient-with-obstructive-azoospermia
#17
Constantin Cristian Văduva, Carmen Constantinescu, Mihaela Mirela Radu, Alice Roxana Văduva, Andrei Pănuş, Mihaela Ţenovici, Damian DiŢescu, Dinu Florin Albu
In this article, we report a case of pregnancy obtained in an infertile couple diagnosed with severe male factor infertility. The couple attended for fertility examination reporting a history of 10 years of infertility. The cause of infertility was obstructive azoospermia. The treatment consists of in vitro fertilization (IVF). The ovarian stimulation of female patient was done with antagonist protocol and after ovarian puncture was obtained nine oocytes. The urologist performed testicular sperm extraction (TESE)...
2016: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/27824942/identifying-maternal-constraints-on-fetal-growth-and-subsequent-perinatal-outcomes-using-a-multiple-embryo-implantation-model
#18
Nigel Pereira, Tyler Cozzubbo, Stephanie Cheung, Zev Rosenwaks, Gianpiero D Palermo, Queenie V Neri
INTRODUCTION: Although the majority of singleton births after in vitro fertilization (IVF) are uncomplicated, studies have suggested that IVF pregnancies may be independently associated with low birth weight (LBW), preterm birth (PTB), and perinatal mortality. These outcomes complicate multiple gestations as expected, but have also been reported in singletons. A multiple embryo implantation model allows for assessment of the early in utero environment, and therefore, assessment of any maternal constraints on developing fetuses...
2016: PloS One
https://www.readbyqxmd.com/read/27821591/rest-is-required-for-nuclear-reprogramming-by-inhibiting-tgf%C3%AE-signaling-pathway
#19
Qing-Ran Kong, Bing-Teng Xie, Heng Zhang, Jing-Yu Li, Tian-Qing Huang, Ren-Yue Wei, Zhong-Hua Liu
Differentiated cells can be reprogrammed by transcription factors, and these factors that are responsible for successful reprogramming need to be further identified. Here, we show that the neuronal repressor REST is rich in porcine oocytes and requires for nuclear transfer (NT)-mediated reprogramming through inhibiting TGFβ signaling pathway. REST was dramatically degraded after oocyte activation, but the residual REST was incorporated into the transferred donor nuclei during reprogramming in NT embryos. Inhibition of REST function in oocytes compromised the development of NT embryos but not that of IVF and PA embryos...
November 7, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27821561/the-ovarian-renin-angiotensin-system-ovras-a-major-factor-in-ovarian-function-and-disease
#20
Angela Palumbo, Julio Ávila, Frederick Naftolin
This contribution summarizes the pivotal role of the ovarian renin-angiotensin system (OVRAS) in ovarian physiology and disease, with particular emphasis on human clinical implications and established translational applications. The presence of a complete OVRAS in all studied species has been known for decades. The OVRAS has major effects on follicle development/atresia and ovulation and steroid hormone secretion, that is, it is necessary for normal reproduction. It is well established that OVRAS activity is regulated by gonadotropins and depends on activation of proteases in the area of growing follicles...
December 2016: Reproductive Sciences
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