keyword
https://read.qxmd.com/read/38542236/follicular-fluid-derived-extracellular-vesicles-influence-on-in-vitro-maturation-of-equine-oocyte-impact-on-cumulus-cell-viability-expansion-and-transcriptome
#1
JOURNAL ARTICLE
Julia Gabryś, Artur Gurgul, Tomasz Szmatoła, Barbara Kij-Mitka, Aneta Andronowska, Elżbieta Karnas, Mirosław Kucharski, Joanna Wojciechowska-Puchałka, Joanna Kochan, Monika Bugno-Poniewierska
Cumulus cell (CC) expansion is pivotal for oocyte maturation, during which CCs release factors that initiate paracrine signaling within the follicular fluid (FF). The FF is abundant in extracellular vesicles (EVs) that facilitate intercellular communication. Although bovine and murine EVs can control cumulus expansion, these effects have not been observed in equines. This study aimed to assess the impact of FF-derived EVs (ffEVs) on equine CC expansion, viability, and transcriptome. Cumulus-oocyte complexes (COCs) that underwent in vitro maturation (IVM) in the presence (200 µg protein/mL) or absence (control) of ffEVs were assessed for cumulus expansion and viability...
March 13, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38540777/molecular-mechanism-of-oocyte-activation-in-mammals-past-present-and-future-directions
#2
REVIEW
Hibiki Sugita, Shunsuke Takarabe, Atsuko Kageyama, Yui Kawata, Junya Ito
During mammalian fertilization, repetitive intracellular Ca2+ increases known as Ca2+ oscillations occur. These oscillations are considered crucial for successful fertilization and subsequent embryonic development. Numerous researchers have endeavored to elucidate the factors responsible for inducing Ca2+ oscillations across various mammalian species. Notably, sperm-specific phospholipase C zeta (PLCζ) emerged as a prominent candidate capable of initiating Ca2+ oscillations, particularly in mammals. Genetic mutation of PLCζ in humans results in the absence of Ca2+ oscillations in mouse oocytes...
March 17, 2024: Biomolecules
https://read.qxmd.com/read/38539996/massively-parallel-crispr-cas9-knockout-screening-in-sheep-granulosa-cells-for-fsh-response-genes
#3
JOURNAL ARTICLE
Zaixia Liu, Lingli Dai, Tianhao Sun, Yongbin Liu, Yanchun Bao, Mingjuan Gu, Shaoyin Fu, Xiaolong He, Caixia Shi, Yu Wang, Lili Guo, Le Zhou, Fengying Ma, Risu Na, Wenguang Zhang
Follicle-stimulating hormone (FSH) regulates ovarian follicle development through specific gene expression programs. Granulosa cells (GCs) are somatic cells surrounding the oocytes, secreting gonadotropins to regulate ovulation and promote follicular development. By analyzing the effects of different doses of FSH on the proliferation of GCs, we found that adding 10 ng/mL of FSH, as the optimal concentration, could promote the growth of GCs. Furthermore, we have successfully constructed the first CRISPR-Cas9 knockout library targeting the genes on chromosomes 2 and 3 and the X chromosomes of the sheep massively parallel coding gene, as well as an ovarian GCs knockout cell library...
March 14, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38539792/advancements-in-genetic-biomarkers-and-exogenous-antioxidant-supplementation-for-safeguarding-mammalian-cells-against-heat-induced-oxidative-stress-and-apoptosis
#4
REVIEW
Muhammad Zahoor Khan, Adnan Khan, Wenting Chen, Wenqiong Chai, Changfa Wang
Heat stress represents a pervasive global concern with far-reaching implications for the reproductive efficiency of both animal and human populations. An extensive body of published research on heat stress effects utilizes controlled experimental environments to expose cells and tissues to heat stress and its disruptive influence on the physiological aspects of reproductive phenotypic traits, encompassing parameters such as sperm quality, sperm motility, viability, and overall competence. Beyond these immediate effects, heat stress has been linked to embryo losses, compromised oocyte development, and even infertility across diverse species...
February 20, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38536780/thc-and-sperm-impact-on-fertilization-capability-pre-implantation-in-vitro-development-and-epigenetic-modifications
#5
JOURNAL ARTICLE
Alexander G Kuzma-Hunt, Reem Sabry, Ola S Davis, Vivien B Truong, Jibran Y Khokhar, Laura A Favetta
Global cannabis use has risen 23% since 2010, with 209 million reported users, most of whom are males of reproductive age. Delta-9-tetrahydrocannabinol (THC), the main psychoactive phytocannabinoid in cannabis, disrupts pro-homeostatic functions of the endocannabinoid system (ECS) within the male reproductive system. The ECS is highly involved in regulating morpho-functional and intrinsic sperm features that are required for fertilization and pre-implantation embryo development. Previous work by our group demonstrated that THC altered sperm capacitation and the transcriptome, including several fertility-associated microRNAs (miRs)...
2024: PloS One
https://read.qxmd.com/read/38536595/identification-novel-mutations-and-phenotypic-spectrum-expanding-in-patl2-in-infertile-women-with-ivf-icsi-failure
#6
JOURNAL ARTICLE
Zhiqi Ye, Da Li, Xiangli Niu, Aimin Yang, Zhiqi Pan, Ran Yu, Hao Gu, Rong Shi, Ling Wu, Yanfang Xiang, Guimin Hao, Yanping Kuang, Biaobang Chen, Lei Wang, Qing Sang, Lin Li, Juanzi Shi, Qiaoli Li
AIM: Abnormalities in oocyte maturation, fertilization, and early embryonic development are major causes of primary infertility in women who are undergoing IVF/ICSI attempts. Although many genetic factors responsible for these abnormal phenotypes have been identified, there are more additional pathogenic genes and variants yet to be discovered. Previous studies confirmed that bi-allelic PATL2 deficiency is an important factor for female infertility. In this study, 935 infertile patients with IVF/ICSI failure were selected for whole-exome sequencing, and 18 probands carrying PATL2 variants with a recessive inheritance pattern were identified...
March 27, 2024: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/38532230/enhancing-oocyte-quality-in-aging-mice-insights-from-mesenchymal-stem-cell-therapy-and-foxo3a-signaling-pathway-activation
#7
JOURNAL ARTICLE
Lingjuan Wang, Yang Liu, Yinhua Song, Qiaojuan Mei, Hongbei Mou, Jiachen Wu, Xinyu Tang, Jihui Ai, Kezhen Li, Houxiu Xiao, Xiaotao Han, Liqun Lv, Huaibiao Li, Ling Zhang, Wenpei Xiang
Ovarian aging reduced the quality of oocytes, resulting in age-related female infertility. It is reported that mesenchymal stem cells (MSCs) therapy can improve age-related ovarian function decline and the success rate of in vitro maturation (IVM) in assisted reproductive therapy. In order to investigate the effectiveness and mechanisms of MSCs to enhance oocyte quality of cumulus oocyte complexes (COCs) in advanced age, this study focus on the respective functional improvement of oocytes and granulosa cells (GCs) from aging mice and further to explore and verify the possible mechanisms...
March 26, 2024: Reproductive Sciences
https://read.qxmd.com/read/38529407/direct-male-development-in-chromosomally-zz-zebrafish
#8
JOURNAL ARTICLE
Catherine A Wilson, Peter Batzel, John H Postlethwait
The genetics of sex determination varies across taxa, sometimes even within a species. Major domesticated strains of zebrafish ( Danio rerio ), including AB and TU, lack a strong genetic sex determining locus, but strains more recently derived from nature, like Nadia (NA), possess a ZZ male/ZW female chromosomal sex-determination system. AB fish pass through a juvenile ovary stage, forming oocytes that survive in fish that become females but die in fish that become males. To understand mechanisms of gonad development in NA zebrafish, we studied histology and single cell transcriptomics in developing ZZ and ZW fish...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38528099/impact-of-media-supplements-fgf2-lif-and-igf1-on-the-genome-activity-of-porcine-embryos-produced-in-vitro
#9
JOURNAL ARTICLE
Alexandra Rosenbaum Bartkova, Lucie Nemcova, Frantisek Strejcek, Ahmed Gad, Veronika Kinterova, Martin Morovic, Michal Benc, Radek Prochazka, Jozef Laurincik
In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium...
March 25, 2024: Scientific Reports
https://read.qxmd.com/read/38527601/embryo-long-term-storage-does-not-affect-art-outcome-analysis-of-58001-vitrified-blastocysts-over-an-11-year-period
#10
JOURNAL ARTICLE
Ana Cobo, Aila Coello, María J DE Los Santos, Jose Remohi, Jose Bellver
BACKGROUND: Recently the potential detrimental effect that the duration of storage time may have on vitrified samples has raised some concern, especially when some studies found an association between cryo-storage length and decreased clinical results. OBJECTIVE: This study aimed to evaluate the effects of the storage time length of day-5 vitrified blastocysts in two study groups: freeze-all cycles and non elective frozen embryo transfers (FET). STUDY DESIGN: This was a retrospective study including 58001 vitrified/warmed day-5 blastocysts from two different populations according to the reason for FET...
March 23, 2024: American Journal of Obstetrics and Gynecology
https://read.qxmd.com/read/38525518/comparative-analysis-of-conventional-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-in-patients-with-polycystic-ovarian-syndrome-tubal-factor-infertility-and-unexplained-infertility-whose-partners-exhibit-normal-semen-parameters-a-retrospective
#11
JOURNAL ARTICLE
Sareh Ashourzadeh, Somayyeh Safari, Robabe Hosseinisadat, Raheleh Kafaeinezhad, Saeed Shokri, Sanaz Alaee
OBJECTIVE: This study compared the outcomes of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in patients with polycystic ovarian syndrome (PCOS), tubal factor (TF) infertility, and unexplained infertility whose partners had normal semen parameters. METHODS: This retrospective study included 360 couples diagnosed with infertility involving PCOS (n=157), unexplained infertility (n=140), and TF infertility (n=63). Sibling oocytes were randomly assigned to undergo ICSI or conventional IVF insemination...
March 25, 2024: Clinical and Experimental Reproductive Medicine
https://read.qxmd.com/read/38524206/comparing-reproductive-outcomes-between-conventional-in%C3%A2-vitro-fertilization-and-nonindicated-intracytoplasmic-sperm-injection-in-autologous-embryo-transfer-cycles-a-society-for-assisted-reproductive-technology-clinic-outcome-reporting-system-study
#12
JOURNAL ARTICLE
Julian A Gingold, Haotian Wu, Harry Lieman, Manvinder Singh, Sangita Jindal
OBJECTIVE: To compare clinical outcomes between nonindicated intracytoplasmic sperm injection (ICSI) and conventional insemination. DESIGN: Autologous cycles performed from 2014-2017 were identified, excluding frozen oocyte cycles. Outcomes were compared between conventional insemination (in vitro fertilization [IVF]) and nonindiated ICSI and analyzed separately for fresh, frozen-thawed preimplantation genetic testing (PGT) and frozen-thawed non-PGT cycles. SETTING: US-based fertility clinics reporting to the Society for Assisted Reproductive Technology...
March 2024: F&S reports
https://read.qxmd.com/read/38520618/obstetric-and-maternal-outcomes-of-ivf-and-oocyte-donation-pregnancies-among-women-ages-40-45-a-large-cohort-study
#13
JOURNAL ARTICLE
Roni Rahav-Koren, Hila Shalev-Ram, Einat Haikin-Herzberger, Mattan Levi, Amir Wiser, Netanella Miller
PURPOSE: To analyze the perinatal and maternal outcomes of women ranging in age from 40 to 45 years who gave birth after in vitro fertilization or oocyte donation, compared to spontaneous conception. METHODS: This retrospective cohort study used electronic data from a national healthcare service from 2000 through 2019. Three groups were compared: spontaneous pregnancy (SC), in vitro fertilization (IVF) utilizing autologous oocytes, and pregnancies resulting from oocyte donation (OD)...
March 23, 2024: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/38511208/the-impact-of-ovarian-stimulation-on-the-human-endometrial-microenvironment
#14
JOURNAL ARTICLE
Anat Chemerinski, May Shen, Nuriban Valero-Pacheco, Qingshi Zhao, Trystn Murphy, Lea George, Alex Lemenze, Lauren Sherman, Debra Heller, Xiaowei Chen, Tracy Wu, Peter G McGovern, Sara S Morelli, Ripla Arora, Aimee M Beaulieu, Nataki C Douglas
STUDY QUESTION: How does ovarian stimulation (OS), which is used to mature multiple oocytes for ART procedures, impact the principal cellular compartments and transcriptome of the human endometrium in the periovulatory and mid-secretory phases? SUMMARY ANSWER: During the mid-secretory window of implantation, OS alters the abundance of endometrial immune cells, whereas during the periovulatory period, OS substantially changes the endometrial transcriptome and impacts both endometrial glandular and immune cells...
March 20, 2024: Human Reproduction
https://read.qxmd.com/read/38510925/neurotensin-induces-sustainable-activation-of-the-erbb-erk1-2-pathway-which-is-required-for-developmental-competence-of-oocytes-in-mice
#15
JOURNAL ARTICLE
Asako Okamoto, Tomoya Nakanishi, Shingo Tonai, Masayuki Shimada, Yasuhisa Yamashita
PURPOSE: LH induces the expression of EGF-like factors and their shedding enzyme (ADAM17) in granulosa cells (GCs), which is essential for ovulation via activation of the ErbB-ERK1/2 pathway in cumulus cells (CCs). Neurotensin (NTS) is reported as a novel regulator of ovulation, whereas the NTS-induced maturation mechanism in oocytes remains unclear. In this study, we focused on the role of NTS in the expression of EGF-like factors and ErbBs, and ADAM17 activity, during oocyte maturation and ovulation in mice...
2024: Reproductive Medicine and Biology
https://read.qxmd.com/read/38508627/latent-class-analysis-of-chinese-healthcare-providers-attitudes-towards-oocyte-cryopreservation-a-cross-sectional-study
#16
JOURNAL ARTICLE
Jingjing Lu, Xuezi Tian, Zhaochen Wang
OBJECTIVES: The present study was designed to examine the attitudes towards oocyte cryopreservation among healthcare providers working in hospitals across specialties and potential influencing factors. DESIGN: A cross-sectional study. SETTING: The questionnaire was distributed among Chinese healthcare providers via the Credamo platform. PARTICIPANTS: There were 877 respondents recruited from 8 April to 8 May 2022, among whom 160 were identified as unqualified because of inconsistency between the IP and work addresses...
March 19, 2024: BMJ Open
https://read.qxmd.com/read/38507112/xpr1-a-regulator-of-cellular-phosphate-homeostasis-rather-than-a-pi-exporter
#17
JOURNAL ARTICLE
David Burns, Rolando Berlinguer-Palmini, Andreas Werner
Phosphate (Pi) is an essential nutrient, and its plasma levels are under tight hormonal control. Uphill transport of Pi into cells is mediated by the two Na-dependent Pi transporter families SLC34 and SLC20. The molecular identity of a potential Pi export pathway is controversial, though XPR1 has recently been suggested by Giovannini and coworkers to mediate Pi export. We expressed XPR1 in Xenopus oocytes to determine its functional characteristics. Xenopus isoforms of proteins were used to avoid species incompatibility...
March 20, 2024: Pflügers Archiv: European Journal of Physiology
https://read.qxmd.com/read/38494512/comparison-of-pregnancy-outcomes-in-women-with-normal-ovarian-response-to-the-gonadotropin-releasing-hormone-agonist-protocol-using-different-trigger-methods-a-single-center-retrospective-cohort-study-based-on-propensity-score-matching
#18
JOURNAL ARTICLE
Danyang Guo, Conghui Pang, Kehua Wang
PURPOSE: To investigate whether gonadotropin-releasing hormone agonist (GnRH-a) combined with human chorionic gonadotropin (HCG) can improve pregnancy outcomes in patients with normal ovarian response (NOR). METHODS: In this retrospective cohort study, data of 404 NOR patients undergoing fresh embryo transfer (ET) from 2018 to 2022 were studied. Patients were divided into HCG group and HCG plus GnRH-a group according to trigger methods. After confounding factors were controlled by propensity score matching, 67 cases were included in HCG group and HCG plus GnRH-a group, respectively, and pregnancy outcomes were assessed...
March 18, 2024: Archives of Gynecology and Obstetrics
https://read.qxmd.com/read/38491313/appropriate-glycemic-management-protects-the-germline-but-not-the-uterine-environment-in-hyperglycemia
#19
JOURNAL ARTICLE
Allan Zhao, Hong Jiang, Arturo Reyes Palomares, Alice Larsson, Wenteng He, Jacob Grünler, Xiaowei Zheng, Kenny A Rodriguez Wallberg, Sergiu-Bogdan Catrina, Qiaolin Deng
Emerging evidence indicates that parental diseases can impact the health of subsequent generations through epigenetic inheritance. Recently, it was shown that maternal diabetes alters the metaphase II oocyte transcriptome, causing metabolic dysfunction in offspring. However, type 1 diabetes (T1D) mouse models frequently utilized in previous studies may be subject to several confounding factors due to severe hyperglycemia. This limits clinical translatability given improvements in glycemic control for T1D subjects...
March 15, 2024: EMBO Reports
https://read.qxmd.com/read/38490113/role-and-action-mechanisms-of-mir-149-and-mir-31-in-regulating-function-of-pig-cumulus-cells-and-oocytes
#20
JOURNAL ARTICLE
Jia-Shun Wu, Shuai Gong, Min Zhang, Rui-Jie Ma, Hui-Li Wang, Ming-Jiu Luo, Nan He, Jing-He Tan
Understanding the mechanisms for oocyte maturation and optimizing the protocols for in vitro maturation (IVM) are greatly important for improving developmental potential of IVM oocytes. The miRNAs expressed in cumulus cells (CCs) play important roles in oocyte maturation and may be used as markers for selection of competent oocytes/embryos. Although a recent study from our group identified several new CCs-expressed miRNAs that regulate cumulus expansion (CE) and CC apoptosis (CCA) in mouse oocytes, validation of these findings and further investigation of mechanisms of action in other model species was essential before wider applications...
March 11, 2024: Theriogenology
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